Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97663.t1 | XP_024636648.1 | 95.9 | 148 | 6 | 0 | 1 | 148 | 148 | 295 | 9.30E-84 | 319.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97663.t1 | Q9SGD1 | 82.7 | 139 | 23 | 1 | 1 | 138 | 197 | 335 | 4.2e-69 | 261.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97663.t1 | A0A396I4F5 | 95.9 | 148 | 6 | 0 | 1 | 148 | 148 | 295 | 6.7e-84 | 319.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene97663.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97663.t1 | MTR_1g093095 | 75.573 | 131 | 27 | 2 | 1 | 126 | 132 | 262 | 3.75e-71 | 216 |
| MS.gene97663.t1 | MTR_1g112270 | 40.789 | 76 | 43 | 2 | 41 | 115 | 133 | 207 | 7.66e-14 | 67.8 |
| MS.gene97663.t1 | MTR_1g112270 | 40.789 | 76 | 43 | 2 | 41 | 115 | 122 | 196 | 9.91e-14 | 67.4 |
| MS.gene97663.t1 | MTR_1g094115 | 39.474 | 76 | 44 | 2 | 41 | 115 | 125 | 199 | 5.01e-13 | 65.5 |
| MS.gene97663.t1 | MTR_4g059870 | 39.560 | 91 | 51 | 3 | 26 | 115 | 104 | 191 | 8.69e-13 | 64.7 |
| MS.gene97663.t1 | MTR_2g093960 | 33.621 | 116 | 71 | 3 | 14 | 125 | 86 | 199 | 1.35e-12 | 64.3 |
| MS.gene97663.t1 | MTR_8g017210 | 39.474 | 76 | 44 | 2 | 41 | 115 | 140 | 214 | 1.28e-11 | 61.6 |
| MS.gene97663.t1 | MTR_1g094115 | 39.474 | 76 | 44 | 2 | 41 | 115 | 108 | 182 | 1.42e-11 | 61.2 |
| MS.gene97663.t1 | MTR_2g090745 | 42.667 | 75 | 40 | 3 | 42 | 115 | 87 | 159 | 1.60e-11 | 61.2 |
| MS.gene97663.t1 | MTR_1g016010 | 35.870 | 92 | 55 | 3 | 25 | 115 | 106 | 194 | 5.21e-11 | 59.7 |
| MS.gene97663.t1 | MTR_2g099990 | 39.474 | 76 | 44 | 2 | 41 | 115 | 55 | 129 | 7.39e-11 | 59.3 |
| MS.gene97663.t1 | MTR_2g099990 | 39.474 | 76 | 44 | 2 | 41 | 115 | 124 | 198 | 7.96e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97663.t1 | AT1G08290 | 82.734 | 139 | 23 | 1 | 1 | 138 | 197 | 335 | 1.49e-82 | 246 |
| MS.gene97663.t1 | AT3G57670 | 76.761 | 142 | 30 | 2 | 1 | 142 | 234 | 372 | 4.19e-77 | 234 |
| MS.gene97663.t1 | AT1G51220 | 84.874 | 119 | 18 | 0 | 1 | 119 | 195 | 313 | 1.06e-76 | 231 |
| MS.gene97663.t1 | AT3G20880 | 73.611 | 144 | 34 | 1 | 1 | 144 | 273 | 412 | 2.30e-76 | 233 |
| MS.gene97663.t1 | AT1G34790 | 70.345 | 145 | 40 | 1 | 1 | 145 | 162 | 303 | 2.52e-72 | 219 |
| MS.gene97663.t1 | AT1G13290 | 80.992 | 121 | 21 | 2 | 1 | 119 | 118 | 238 | 3.68e-71 | 216 |
| MS.gene97663.t1 | AT1G34370 | 38.411 | 151 | 76 | 7 | 1 | 142 | 51 | 193 | 7.03e-21 | 86.3 |
| MS.gene97663.t1 | AT1G34370 | 38.411 | 151 | 76 | 7 | 1 | 142 | 261 | 403 | 2.24e-20 | 86.7 |
| MS.gene97663.t1 | AT1G34370 | 38.411 | 151 | 76 | 7 | 1 | 142 | 261 | 403 | 2.24e-20 | 86.7 |
| MS.gene97663.t1 | AT5G22890 | 39.344 | 122 | 67 | 4 | 1 | 115 | 234 | 355 | 1.41e-18 | 80.9 |
| MS.gene97663.t1 | AT5G66730 | 40.000 | 85 | 46 | 3 | 32 | 115 | 104 | 184 | 1.37e-14 | 70.1 |
| MS.gene97663.t1 | AT2G02080 | 40.789 | 76 | 43 | 2 | 41 | 115 | 132 | 206 | 4.19e-14 | 68.6 |
| MS.gene97663.t1 | AT2G02080 | 40.789 | 76 | 43 | 2 | 41 | 115 | 132 | 206 | 4.19e-14 | 68.6 |
| MS.gene97663.t1 | AT2G02080 | 40.789 | 76 | 43 | 2 | 41 | 115 | 132 | 206 | 4.19e-14 | 68.6 |
| MS.gene97663.t1 | AT2G02080 | 40.789 | 76 | 43 | 2 | 41 | 115 | 132 | 206 | 4.19e-14 | 68.6 |
| MS.gene97663.t1 | AT5G03150 | 39.535 | 86 | 47 | 3 | 31 | 115 | 125 | 206 | 7.75e-13 | 65.1 |
| MS.gene97663.t1 | AT1G14580 | 42.105 | 76 | 42 | 2 | 41 | 115 | 145 | 219 | 5.98e-12 | 62.4 |
| MS.gene97663.t1 | AT1G14580 | 42.105 | 76 | 42 | 2 | 41 | 115 | 131 | 205 | 6.30e-12 | 62.4 |
| MS.gene97663.t1 | AT1G14580 | 42.105 | 76 | 42 | 2 | 41 | 115 | 131 | 205 | 6.30e-12 | 62.4 |
| MS.gene97663.t1 | AT1G55110 | 37.634 | 93 | 52 | 4 | 25 | 115 | 128 | 216 | 6.32e-12 | 62.4 |
| MS.gene97663.t1 | AT1G55110 | 37.634 | 93 | 52 | 4 | 25 | 115 | 128 | 216 | 6.32e-12 | 62.4 |
| MS.gene97663.t1 | AT1G55110 | 37.634 | 93 | 52 | 4 | 25 | 115 | 128 | 216 | 6.32e-12 | 62.4 |
| MS.gene97663.t1 | AT2G02080 | 40.789 | 76 | 43 | 2 | 41 | 115 | 55 | 129 | 7.51e-12 | 62.0 |
| MS.gene97663.t1 | AT2G02080 | 40.789 | 76 | 43 | 2 | 41 | 115 | 55 | 129 | 7.51e-12 | 62.0 |
| MS.gene97663.t1 | AT2G02070 | 40.789 | 76 | 43 | 2 | 41 | 115 | 130 | 204 | 9.55e-12 | 62.0 |
| MS.gene97663.t1 | AT2G02070 | 40.789 | 76 | 43 | 2 | 41 | 115 | 130 | 204 | 9.55e-12 | 62.0 |
| MS.gene97663.t1 | AT3G45260 | 40.230 | 87 | 48 | 3 | 30 | 115 | 109 | 192 | 1.19e-11 | 61.6 |
| MS.gene97663.t1 | AT3G45260 | 40.230 | 87 | 48 | 3 | 30 | 115 | 109 | 192 | 1.19e-11 | 61.6 |
| MS.gene97663.t1 | AT5G44160 | 37.363 | 91 | 53 | 3 | 26 | 115 | 63 | 150 | 1.58e-11 | 61.2 |
| MS.gene97663.t1 | AT5G44160 | 37.363 | 91 | 53 | 3 | 26 | 115 | 102 | 189 | 1.83e-11 | 61.2 |
| MS.gene97663.t1 | AT5G60470 | 40.230 | 87 | 48 | 3 | 30 | 115 | 105 | 188 | 1.91e-11 | 60.8 |
| MS.gene97663.t1 | AT5G60470 | 40.230 | 87 | 48 | 3 | 30 | 115 | 114 | 197 | 2.20e-11 | 60.8 |
| MS.gene97663.t1 | AT5G60470 | 40.230 | 87 | 48 | 3 | 30 | 115 | 110 | 193 | 2.44e-11 | 60.8 |
| MS.gene97663.t1 | AT1G03840 | 37.363 | 91 | 53 | 3 | 26 | 115 | 106 | 193 | 2.54e-11 | 60.8 |
| MS.gene97663.t1 | AT1G03840 | 37.363 | 91 | 53 | 3 | 26 | 115 | 104 | 191 | 2.57e-11 | 60.5 |
Find 28 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATCATATTAGGTCTTT+TGG | 0.190747 | 4.4:-49059523 | MS.gene97663:CDS |
| CCATGCTACTGTTGTGTTCA+AGG | 0.271424 | 4.4:-49059763 | MS.gene97663:CDS |
| GAGGAGAAGGGATGTGTTAC+TGG | 0.312071 | 4.4:-49059454 | MS.gene97663:CDS |
| GATCTCTCAAAGATCATATT+AGG | 0.336377 | 4.4:-49059531 | MS.gene97663:CDS |
| TCTTTCAAAGGCTTAGCTCT+TGG | 0.410923 | 4.4:+49059718 | None:intergenic |
| CATTTGCTGTCAAAGGTGAT+TGG | 0.415877 | 4.4:-49059621 | MS.gene97663:CDS |
| TGTGTGCCTCTTAGTGAGTC+TGG | 0.423624 | 4.4:+49059808 | None:intergenic |
| AAAAGAATTGTGGCAAGTTA+TGG | 0.431308 | 4.4:-49059588 | MS.gene97663:CDS |
| CCATTTATGTGTAGAAAATG+TGG | 0.488125 | 4.4:-49059649 | MS.gene97663:CDS |
| GGTAGCCTTAGCATTGCAGC+TGG | 0.491825 | 4.4:+49059784 | None:intergenic |
| TCAAGCTTTCGAAGAGGAGA+AGG | 0.518310 | 4.4:-49059467 | MS.gene97663:CDS |
| AAGTTATGGTATTGCACTTG+TGG | 0.518903 | 4.4:-49059574 | MS.gene97663:CDS |
| TTTCCATTAGATGCATATGT+GGG | 0.526101 | 4.4:-49059857 | None:intergenic |
| TTGCTGTCAAAGGTGATTGG+AGG | 0.526560 | 4.4:-49059618 | MS.gene97663:CDS |
| CACAACCAGCTGCAATGCTA+AGG | 0.533499 | 4.4:-49059789 | MS.gene97663:CDS |
| AGAGTTCTAAAGTCTTTCAA+AGG | 0.540762 | 4.4:+49059706 | None:intergenic |
| TCCATTAGATGCATATGTGG+GGG | 0.571799 | 4.4:-49059855 | None:intergenic |
| AAAGGACCAGACTCACTAAG+AGG | 0.575770 | 4.4:-49059814 | MS.gene97663:CDS |
| TTCCATTAGATGCATATGTG+GGG | 0.605042 | 4.4:-49059856 | None:intergenic |
| CATTTATGTGTAGAAAATGT+GGG | 0.610048 | 4.4:-49059648 | MS.gene97663:CDS |
| ACTCACTACAAAAGAAAACA+TGG | 0.615968 | 4.4:-49059679 | MS.gene97663:CDS |
| CAAGCTTTCGAAGAGGAGAA+GGG | 0.617403 | 4.4:-49059466 | MS.gene97663:CDS |
| AAAGCTTGATCATCAATCGA+AGG | 0.619722 | 4.4:+49059481 | None:intergenic |
| AGGACTCATGAAAAGAATTG+TGG | 0.623632 | 4.4:-49059598 | MS.gene97663:CDS |
| GGGAAAACATTTGCTGTCAA+AGG | 0.623807 | 4.4:-49059628 | MS.gene97663:CDS |
| CATGGATCTGAATTTAGGAA+AGG | 0.631532 | 4.4:-49059832 | MS.gene97663:CDS |
| TGATGATCAAGCTTTCGAAG+AGG | 0.631618 | 4.4:-49059473 | MS.gene97663:CDS |
| CCTTGAACACAACAGTAGCA+TGG | 0.671135 | 4.4:+49059763 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CATTTATGTGTAGAAAATGT+GGG | - | chr4.4:49059622-49059641 | MS.gene97663:CDS | 25.0% |
| !! | AAAGATCATATTAGGTCTTT+TGG | - | chr4.4:49059747-49059766 | MS.gene97663:CDS | 25.0% |
| AAAAGAATTGTGGCAAGTTA+TGG | - | chr4.4:49059682-49059701 | MS.gene97663:CDS | 30.0% | |
| ACTCACTACAAAAGAAAACA+TGG | - | chr4.4:49059591-49059610 | MS.gene97663:CDS | 30.0% | |
| CCATTTATGTGTAGAAAATG+TGG | - | chr4.4:49059621-49059640 | MS.gene97663:CDS | 30.0% | |
| GATCTCTCAAAGATCATATT+AGG | - | chr4.4:49059739-49059758 | MS.gene97663:CDS | 30.0% | |
| ! | CCACATTTTCTACACATAAA+TGG | + | chr4.4:49059624-49059643 | None:intergenic | 30.0% |
| ! | GTTTTCTTTTGTAGTGAGTT+TGG | + | chr4.4:49059590-49059609 | None:intergenic | 30.0% |
| !! | AGAGTTCTAAAGTCTTTCAA+AGG | + | chr4.4:49059567-49059586 | None:intergenic | 30.0% |
| !! | CATATTAGGTCTTTTGGAAA+AGG | - | chr4.4:49059753-49059772 | MS.gene97663:CDS | 30.0% |
| AAAGCTTGATCATCAATCGA+AGG | + | chr4.4:49059792-49059811 | None:intergenic | 35.0% | |
| AAGTTATGGTATTGCACTTG+TGG | - | chr4.4:49059696-49059715 | MS.gene97663:CDS | 35.0% | |
| AGGACTCATGAAAAGAATTG+TGG | - | chr4.4:49059672-49059691 | MS.gene97663:CDS | 35.0% | |
| CATGGATCTGAATTTAGGAA+AGG | - | chr4.4:49059438-49059457 | MS.gene97663:CDS | 35.0% | |
| TGATGATCAAGCTTTCGAAG+AGG | - | chr4.4:49059797-49059816 | MS.gene97663:CDS | 40.0% | |
| ! | TCTTTCAAAGGCTTAGCTCT+TGG | + | chr4.4:49059555-49059574 | None:intergenic | 40.0% |
| !! | CATTTGCTGTCAAAGGTGAT+TGG | - | chr4.4:49059649-49059668 | MS.gene97663:CDS | 40.0% |
| !! | GGGAAAACATTTGCTGTCAA+AGG | - | chr4.4:49059642-49059661 | MS.gene97663:CDS | 40.0% |
| AAAGGACCAGACTCACTAAG+AGG | - | chr4.4:49059456-49059475 | MS.gene97663:CDS | 45.0% | |
| CAAGCTTTCGAAGAGGAGAA+GGG | - | chr4.4:49059804-49059823 | MS.gene97663:CDS | 45.0% | |
| CCATGCTACTGTTGTGTTCA+AGG | - | chr4.4:49059507-49059526 | MS.gene97663:CDS | 45.0% | |
| CCTTGAACACAACAGTAGCA+TGG | + | chr4.4:49059510-49059529 | None:intergenic | 45.0% | |
| TCAAGCTTTCGAAGAGGAGA+AGG | - | chr4.4:49059803-49059822 | MS.gene97663:CDS | 45.0% | |
| TTGCTGTCAAAGGTGATTGG+AGG | - | chr4.4:49059652-49059671 | MS.gene97663:CDS | 45.0% | |
| CACAACCAGCTGCAATGCTA+AGG | - | chr4.4:49059481-49059500 | MS.gene97663:CDS | 50.0% | |
| GAGGAGAAGGGATGTGTTAC+TGG | - | chr4.4:49059816-49059835 | MS.gene97663:CDS | 50.0% | |
| TGTGTGCCTCTTAGTGAGTC+TGG | + | chr4.4:49059465-49059484 | None:intergenic | 50.0% | |
| ! | GGGGGCATGGATCTGAATTT+AGG | - | chr4.4:49059433-49059452 | MS.gene97663:CDS | 50.0% |
| ! | GGTAGCCTTAGCATTGCAGC+TGG | + | chr4.4:49059489-49059508 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 49059423 | 49059869 | 49059423 | ID=MS.gene97663 |
| chr4.4 | mRNA | 49059423 | 49059869 | 49059423 | ID=MS.gene97663.t1;Parent=MS.gene97663 |
| chr4.4 | exon | 49059423 | 49059869 | 49059423 | ID=MS.gene97663.t1.exon1;Parent=MS.gene97663.t1 |
| chr4.4 | CDS | 49059423 | 49059869 | 49059423 | ID=cds.MS.gene97663.t1;Parent=MS.gene97663.t1 |
| Gene Sequence |
| Protein sequence |
