AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97699

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97699.t1 MTR_5g029840 95.935 246 10 0 37 282 89 334 9.48e-171 475
MS.gene97699.t1 MTR_4g478180 33.333 276 135 9 37 282 90 346 8.11e-26 104
MS.gene97699.t1 MTR_3g074520 48.980 98 40 2 37 124 89 186 1.77e-21 92.8
MS.gene97699.t1 MTR_5g007370 33.465 254 118 12 37 282 89 299 8.17e-21 90.1
MS.gene97699.t1 MTR_2g089620 62.687 67 25 0 37 103 89 155 4.64e-19 85.9
MS.gene97699.t1 MTR_6g009430 45.455 110 49 3 37 146 89 187 4.77e-18 83.2
MS.gene97699.t1 MTR_4g073420 62.069 58 22 0 37 94 89 146 7.28e-17 79.0
MS.gene97699.t1 MTR_2g011660 80.488 41 8 0 37 77 90 130 1.61e-16 79.0
MS.gene97699.t1 MTR_5g078140 85.000 40 6 0 37 76 89 128 1.92e-16 78.2
MS.gene97699.t1 MTR_5g007310 27.799 259 130 11 46 282 6 229 1.16e-15 74.7
MS.gene97699.t1 MTR_3g065440 84.615 39 6 0 37 75 89 127 1.92e-15 75.5
MS.gene97699.t1 MTR_4g102380 80.000 40 8 0 37 76 89 128 2.06e-15 74.7
MS.gene97699.t1 MTR_4g082290 68.889 45 14 0 37 81 89 133 2.18e-15 75.9
MS.gene97699.t1 MTR_4g091490 31.064 235 116 6 37 235 90 314 2.41e-15 75.5
MS.gene97699.t1 MTR_4g082230 70.455 44 13 0 37 80 89 132 2.44e-15 75.9
MS.gene97699.t1 MTR_4g105130 72.093 43 12 0 37 79 89 131 3.80e-15 75.1
MS.gene97699.t1 MTR_6g012690 72.093 43 12 0 37 79 89 131 6.16e-15 74.3
MS.gene97699.t1 MTR_7g011170 59.649 57 19 1 37 89 89 145 6.90e-15 73.9
MS.gene97699.t1 MTR_3g077110 72.500 40 11 0 37 76 89 128 1.05e-14 73.6
MS.gene97699.t1 MTR_1g112760 60.417 48 19 0 37 84 90 137 1.26e-14 72.0
MS.gene97699.t1 MTR_5g042030 71.429 42 12 0 37 78 84 125 1.98e-14 72.4
MS.gene97699.t1 MTR_3g461490 41.905 105 52 6 37 138 89 187 2.59e-14 72.4
MS.gene97699.t1 MTR_5g079670 50.000 64 32 0 37 100 88 151 1.04e-13 68.9
MS.gene97699.t1 MTR_8g095390 72.500 40 11 0 37 76 89 128 2.20e-13 69.3
MS.gene97699.t1 MTR_8g031360 67.500 40 13 0 37 76 91 130 2.55e-13 68.9
MS.gene97699.t1 MTR_3g083540 72.500 40 11 0 37 76 118 157 2.92e-13 68.9
MS.gene97699.t1 MTR_3g097450 67.442 43 14 0 37 79 89 131 2.95e-13 68.9
MS.gene97699.t1 MTR_3g045430 41.000 100 49 4 37 130 90 185 5.49e-13 68.2
MS.gene97699.t1 MTR_7g076740 65.000 40 14 0 37 76 90 129 5.87e-13 68.2
MS.gene97699.t1 MTR_7g110830 61.702 47 18 0 37 83 89 135 7.90e-13 68.2
MS.gene97699.t1 MTR_1g085040 65.116 43 15 0 37 79 89 131 1.44e-12 67.4
MS.gene97699.t1 MTR_1g085640 65.116 43 15 0 37 79 89 131 1.62e-12 67.4
MS.gene97699.t1 MTR_2g064160 65.789 38 13 0 37 74 88 125 1.75e-12 67.0
MS.gene97699.t1 MTR_4g485530 71.053 38 11 0 37 74 89 126 2.93e-12 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97699.t1 AT5G16770 38.023 263 120 11 37 282 89 325 7.85e-32 120
MS.gene97699.t1 AT5G16770 38.023 263 120 11 37 282 89 325 7.85e-32 120
MS.gene97699.t1 AT5G16770 38.023 263 120 11 37 282 89 325 9.96e-32 120
MS.gene97699.t1 AT5G16770 38.023 263 120 11 37 282 89 325 9.96e-32 120
MS.gene97699.t1 AT4G17785 36.364 275 131 14 37 282 90 349 3.30e-30 116
MS.gene97699.t1 AT4G17785 36.000 275 132 14 37 282 90 349 1.08e-29 115
MS.gene97699.t1 AT3G02940 33.462 260 121 8 37 282 89 310 6.30e-29 112
MS.gene97699.t1 AT3G02940 33.462 260 121 8 37 282 89 310 6.66e-29 112
MS.gene97699.t1 AT3G02940 33.462 260 121 8 37 282 89 310 6.66e-29 112
MS.gene97699.t1 AT5G10280 51.546 97 35 4 37 124 89 182 6.38e-21 91.3
MS.gene97699.t1 AT1G34670 48.315 89 42 2 37 123 89 175 1.11e-19 88.2
MS.gene97699.t1 AT5G65230 47.059 119 47 2 11 124 74 181 6.83e-19 85.1
MS.gene97699.t1 AT4G21440 68.421 57 15 1 37 93 89 142 1.34e-18 84.7
MS.gene97699.t1 AT5G54230 68.421 57 15 1 37 93 89 142 2.05e-17 81.3
MS.gene97699.t1 AT4G05100 85.366 41 6 0 37 77 90 130 3.66e-17 80.5
MS.gene97699.t1 AT1G66230 85.000 40 6 0 37 76 89 128 1.45e-16 78.2
MS.gene97699.t1 AT4G28110 75.610 41 10 0 37 77 5 45 1.49e-16 76.6
MS.gene97699.t1 AT5G16600 85.000 40 6 0 37 76 89 128 2.18e-16 78.2
MS.gene97699.t1 AT5G15310 72.093 43 12 0 37 79 10 52 2.71e-16 77.0
MS.gene97699.t1 AT4G12350 58.462 65 24 1 37 101 89 150 5.83e-16 76.6
MS.gene97699.t1 AT5G15310 72.093 43 12 0 37 79 89 131 8.84e-16 75.5
MS.gene97699.t1 AT4G22680 82.500 40 7 0 37 76 89 128 1.00e-15 75.5
MS.gene97699.t1 AT5G15310 72.093 43 12 0 37 79 89 131 1.54e-15 75.9
MS.gene97699.t1 AT5G15310 72.093 43 12 0 37 79 89 131 1.59e-15 75.9
MS.gene97699.t1 AT3G13540 77.500 40 9 0 37 76 100 139 1.91e-15 74.7
MS.gene97699.t1 AT5G14340 65.217 46 16 0 37 82 11 56 2.22e-15 73.2
MS.gene97699.t1 AT4G28110 75.610 41 10 0 37 77 89 129 2.99e-15 74.3
MS.gene97699.t1 AT3G01140 55.385 65 24 1 37 101 89 148 7.54e-15 73.9
MS.gene97699.t1 AT3G01140 55.385 65 24 1 37 101 132 191 9.17e-15 73.9
MS.gene97699.t1 AT5G61420 72.500 40 11 0 37 76 10 49 1.39e-14 72.8
MS.gene97699.t1 AT5G62320 74.359 39 10 0 37 75 97 135 1.47e-14 72.0
MS.gene97699.t1 AT5G56110 67.347 49 13 1 37 85 89 134 1.48e-14 72.8
MS.gene97699.t1 AT5G14340 65.217 46 16 0 37 82 101 146 1.53e-14 72.4
MS.gene97699.t1 AT4G34990 72.500 40 11 0 37 76 89 128 2.72e-14 71.6
MS.gene97699.t1 AT1G57560 64.706 51 18 0 37 87 89 139 2.89e-14 71.6
MS.gene97699.t1 AT1G57560 59.016 61 16 1 37 88 89 149 3.93e-14 71.6
MS.gene97699.t1 AT3G61250 65.909 44 15 0 37 80 89 132 4.36e-14 71.2
MS.gene97699.t1 AT5G61420 72.500 40 11 0 37 76 89 128 6.48e-14 71.6
MS.gene97699.t1 AT4G38620 72.500 40 11 0 37 76 89 128 6.64e-14 70.9
MS.gene97699.t1 AT4G09460 50.649 77 25 2 10 75 53 127 1.05e-13 69.3
MS.gene97699.t1 AT2G16720 76.923 39 9 0 37 75 89 127 1.14e-13 69.7
MS.gene97699.t1 AT1G22640 71.795 39 11 0 37 75 17 55 1.88e-13 67.8
MS.gene97699.t1 AT1G18570 76.923 39 9 0 37 75 90 128 2.05e-13 70.1
MS.gene97699.t1 AT1G22640 71.795 39 11 0 37 75 32 70 3.57e-13 67.4
MS.gene97699.t1 AT1G35515 64.000 50 14 1 37 82 89 138 3.92e-13 67.4
MS.gene97699.t1 AT5G26660 70.000 40 12 0 37 76 58 97 3.99e-13 68.9
MS.gene97699.t1 AT5G07700 70.000 40 12 0 37 76 89 128 4.50e-13 68.9
MS.gene97699.t1 AT5G07700 70.000 40 12 0 37 76 89 128 4.50e-13 68.9
MS.gene97699.t1 AT5G26660 70.000 40 12 0 37 76 89 128 6.48e-13 68.6
MS.gene97699.t1 AT5G07690 72.500 40 11 0 37 76 89 128 7.08e-13 68.2
MS.gene97699.t1 AT1G09540 72.500 40 11 0 37 76 89 128 7.36e-13 68.6
MS.gene97699.t1 AT1G22640 71.795 39 11 0 37 75 89 127 9.83e-13 67.0
MS.gene97699.t1 AT3G12720 68.421 38 12 0 37 74 99 136 3.33e-12 65.9
MS.gene97699.t1 AT5G60890 67.500 40 13 0 37 76 89 128 1.61e-11 63.9
MS.gene97699.t1 AT4G01680 67.442 43 14 0 37 79 89 131 2.19e-11 62.8
MS.gene97699.t1 AT4G01680 67.442 43 14 0 37 79 101 143 2.66e-11 63.5
MS.gene97699.t1 AT4G01680 67.442 43 14 0 37 79 89 131 2.85e-11 63.5
MS.gene97699.t1 AT1G74430 57.143 42 18 0 37 78 20 61 6.57e-11 60.8
MS.gene97699.t1 AT1G74080 65.116 43 15 0 37 79 89 131 8.31e-11 62.0

Find 59 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTTGGCTTGTGAGTTTC+AGG 0.124801 5.1:-20455062 None:intergenic
CAAGTTACCAAATGAATTAT+TGG 0.200841 5.1:-20455234 None:intergenic
AATTATCCTTATAACCCTTT+AGG 0.212430 5.1:+20455260 MS.gene97699:CDS
TCACCACCACTCAAACTTAA+TGG 0.223472 5.1:-20455353 None:intergenic
AACCCTAGTTTATACTCTTA+TGG 0.226899 5.1:+20455389 MS.gene97699:CDS
CAATAATTCATTTGGTAACT+TGG 0.256156 5.1:+20455235 MS.gene97699:CDS
TGATTGAAAATTCCAGTTCT+AGG 0.257401 5.1:-20455545 None:intergenic
ATTATCCTTATAACCCTTTA+GGG 0.266138 5.1:+20455261 MS.gene97699:CDS
AGATCTTGATTATAGCAAAA+TGG 0.266341 5.1:+20455463 MS.gene97699:CDS
ATAACGAGATAAAGAATTAT+TGG 0.272931 5.1:+20454997 MS.gene97699:CDS
CAAATGCAATCTTTGATGAT+TGG 0.288485 5.1:+20455618 MS.gene97699:CDS
AGAAAATCTACTTCCTGCTT+TGG 0.327301 5.1:+20455511 MS.gene97699:CDS
ATGAATACCAATAATTCATT+TGG 0.331994 5.1:+20455227 MS.gene97699:CDS
AAATGCAATCTTTGATGATT+GGG 0.348856 5.1:+20455619 MS.gene97699:CDS
AGGTGATTGTAATCAGTTCT+TGG 0.348925 5.1:-20455077 None:intergenic
AAAATTGCAGCTCATCTTCC+AGG 0.357488 5.1:+20454966 MS.gene97699:CDS
ATATTCCCTAAAGGGTTATA+AGG 0.369429 5.1:-20455266 None:intergenic
GTTGCTTCCTCTCCTAGAAC+TGG 0.376485 5.1:+20455533 MS.gene97699:CDS
TTACGCTTCAACACCTTCCT+AGG 0.377814 5.1:-20453463 None:intergenic
TTACTTGGCATGTCAAATAT+AGG 0.382706 5.1:+20455116 MS.gene97699:CDS
AATCAAGTGGTGGCTCCTAC+TGG 0.391590 5.1:+20455660 MS.gene97699:CDS
TAGGGTTAATATTCCCTAAA+GGG 0.396001 5.1:-20455274 None:intergenic
GATCTTGATTATAGCAAAAT+GGG 0.401633 5.1:+20455464 MS.gene97699:CDS
AATAATTCATTTGGTAACTT+GGG 0.423937 5.1:+20455236 MS.gene97699:CDS
CTGCCAGAGCACACCATTGA+AGG 0.428738 5.1:-20453510 None:intergenic
ATGAGTCTTTCAAACTTACT+TGG 0.438102 5.1:+20455101 MS.gene97699:CDS
CTAGGGTTAATATTCCCTAA+AGG 0.452184 5.1:-20455275 None:intergenic
AGGAGTATTTATACAATCCT+AGG 0.463093 5.1:+20453446 MS.gene97699:CDS
TCATCAAAGATTGCATTTGA+AGG 0.464583 5.1:-20455614 None:intergenic
GGAAGGTGTTGAAGCGTAAA+GGG 0.472623 5.1:+20453467 MS.gene97699:CDS
CTCCTTCTCACAACAATACA+AGG 0.474894 5.1:-20453428 None:intergenic
TGTCCATAAGAGTATAAACT+AGG 0.478465 5.1:-20455392 None:intergenic
ATCTCGTTATCTGTTCGTCC+TGG 0.479112 5.1:-20454984 None:intergenic
TCAAAGCTGGATATTTCTAA+AGG 0.481301 5.1:+20455425 MS.gene97699:CDS
GGAGAGGAAGCAACCAAAGC+AGG 0.484843 5.1:-20455524 None:intergenic
AGGAAGGTGTTGAAGCGTAA+AGG 0.499427 5.1:+20453466 MS.gene97699:CDS
ATTATTGGAACACAAACATA+AGG 0.499952 5.1:+20455012 MS.gene97699:CDS
AAATTGAATGGATTAAAGCT+AGG 0.500163 5.1:-20455293 None:intergenic
AATTGAATGGATTAAAGCTA+GGG 0.513125 5.1:-20455292 None:intergenic
ACAAAGTGAGTCTTCAAAGC+TGG 0.515723 5.1:+20455412 MS.gene97699:CDS
GTAAGTTTGAAAGACTCATA+AGG 0.528357 5.1:-20455097 None:intergenic
ATAATTCATTTGGTAACTTG+GGG 0.539650 5.1:+20455237 MS.gene97699:CDS
AAGCTGGATATTTCTAAAGG+TGG 0.543522 5.1:+20455428 MS.gene97699:CDS
ATTTGAAGGTGAGTGATCAA+TGG 0.559476 5.1:-20455600 None:intergenic
TTACAGACGACACCCTTCAA+TGG 0.567242 5.1:+20453497 MS.gene97699:CDS
GTCCATAAGAGTATAAACTA+GGG 0.567531 5.1:-20455391 None:intergenic
TGCCAGAGCACACCATTGAA+GGG 0.568315 5.1:-20453509 None:intergenic
CACCACTTGATTCGTCGTCA+AGG 0.586781 5.1:-20455649 None:intergenic
TACCTTGTATTGTTGTGAGA+AGG 0.588993 5.1:+20453426 MS.gene97699:CDS
AATGAGCCATTAAGTTTGAG+TGG 0.592575 5.1:+20455347 MS.gene97699:CDS
CTCTAAAAGCTCTTTCCAGT+AGG 0.609003 5.1:-20455675 None:intergenic
TATCTTGTTGTAAACCAAGA+GGG 0.613559 5.1:-20455157 None:intergenic
GAAAATTCCAGTTCTAGGAG+AGG 0.625567 5.1:-20455540 None:intergenic
GTATTTATACAATCCTAGGA+AGG 0.646478 5.1:+20453450 MS.gene97699:CDS
TTCCTTGACGACGAATCAAG+TGG 0.658330 5.1:+20455647 MS.gene97699:CDS
ATATCTTGTTGTAAACCAAG+AGG 0.662547 5.1:-20455158 None:intergenic
AGGTGTTGAAGCGTAAAGGG+AGG 0.695412 5.1:+20453470 MS.gene97699:CDS
CTTGACGACGAATCAAGTGG+TGG 0.701335 5.1:+20455650 MS.gene97699:CDS
GAGCCATTAAGTTTGAGTGG+TGG 0.713030 5.1:+20455350 MS.gene97699:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAATTAGCAAAAATGA+TGG + chr5.1:20454278-20454297 MS.gene97699:intron 15.0%
!! ATTGATTATATCAACAAAAA+TGG + chr5.1:20454498-20454517 MS.gene97699:intron 15.0%
!!! ATTTTTTTTATTTGTCTTGT+AGG + chr5.1:20454937-20454956 MS.gene97699:intron 15.0%
!!! CAATTTTTTCTAGTATAAAA+GGG - chr5.1:20454760-20454779 None:intergenic 15.0%
!!! TCAATTTTTTCTAGTATAAA+AGG - chr5.1:20454761-20454780 None:intergenic 15.0%
!!! TGTCGTTTAATTAAAAATAA+AGG - chr5.1:20454090-20454109 None:intergenic 15.0%
!! AAAATCTTCAAATAATGTTG+AGG + chr5.1:20453943-20453962 MS.gene97699:intron 20.0%
!! AAATTTAGTATAACTGGATT+AGG - chr5.1:20454122-20454141 None:intergenic 20.0%
!! ACTATGAAATTTAGTATAAC+TGG - chr5.1:20454128-20454147 None:intergenic 20.0%
!! ATAACGAGATAAAGAATTAT+TGG + chr5.1:20454997-20455016 MS.gene97699:CDS 20.0%
!! ATGAATACCAATAATTCATT+TGG + chr5.1:20455227-20455246 MS.gene97699:CDS 20.0%
!! GAAATATTGACAAAAATCAA+AGG - chr5.1:20454869-20454888 None:intergenic 20.0%
!!! AATAATTCATTTGGTAACTT+GGG + chr5.1:20455236-20455255 MS.gene97699:CDS 20.0%
!!! AATATTATGTGGGATTTTAT+AGG - chr5.1:20454333-20454352 None:intergenic 20.0%
!!! ATATTTAGCACATTTTATGA+TGG - chr5.1:20453668-20453687 None:intergenic 20.0%
! AAGACAAAACTATAGGTTAT+AGG - chr5.1:20454374-20454393 None:intergenic 25.0%
! AATGTTGAGGAAAACATTAT+TGG + chr5.1:20453956-20453975 MS.gene97699:intron 25.0%
! AATTATCCTTATAACCCTTT+AGG + chr5.1:20455260-20455279 MS.gene97699:CDS 25.0%
! AGATCTTGATTATAGCAAAA+TGG + chr5.1:20455463-20455482 MS.gene97699:CDS 25.0%
! ATTATCCTTATAACCCTTTA+GGG + chr5.1:20455261-20455280 MS.gene97699:CDS 25.0%
! ATTATTGGAACACAAACATA+AGG + chr5.1:20455012-20455031 MS.gene97699:CDS 25.0%
! CAAGTTACCAAATGAATTAT+TGG - chr5.1:20455237-20455256 None:intergenic 25.0%
! CCATTCAAAAACAAATTGAA+TGG - chr5.1:20455308-20455327 None:intergenic 25.0%
! CGCAGAATTTAATTATGAAA+AGG - chr5.1:20453828-20453847 None:intergenic 25.0%
! GATCTTGATTATAGCAAAAT+GGG + chr5.1:20455464-20455483 MS.gene97699:CDS 25.0%
! TCACTGTGCAATATTATTAT+GGG + chr5.1:20454405-20454424 MS.gene97699:intron 25.0%
! TCCTGATCATAAAACAAAAA+AGG + chr5.1:20453697-20453716 MS.gene97699:intron 25.0%
! TTCACTGTGCAATATTATTA+TGG + chr5.1:20454404-20454423 MS.gene97699:intron 25.0%
!! AAATGCAATCTTTGATGATT+GGG + chr5.1:20455619-20455638 MS.gene97699:CDS 25.0%
!! AACTGGAATTTTCAATCAAA+TGG + chr5.1:20455550-20455569 MS.gene97699:CDS 25.0%
!! ATAATTCATTTGGTAACTTG+GGG + chr5.1:20455237-20455256 MS.gene97699:CDS 25.0%
!! ATTTTGCAACAATTGCATTT+TGG - chr5.1:20455195-20455214 None:intergenic 25.0%
!! CAATAATTCATTTGGTAACT+TGG + chr5.1:20455235-20455254 MS.gene97699:CDS 25.0%
!!! AAATTGAATGGATTAAAGCT+AGG - chr5.1:20455296-20455315 None:intergenic 25.0%
!!! AATTGAATGGATTAAAGCTA+GGG - chr5.1:20455295-20455314 None:intergenic 25.0%
!!! ACCTTTTTTGTTTTATGATC+AGG - chr5.1:20453701-20453720 None:intergenic 25.0%
!!! ATTTTGGCATTTTTCATCTA+AGG - chr5.1:20454180-20454199 None:intergenic 25.0%
!!! CCATTCAATTTGTTTTTGAA+TGG + chr5.1:20455305-20455324 MS.gene97699:CDS 25.0%
!!! TATAACCTATAGTTTTGTCT+TGG + chr5.1:20454373-20454392 MS.gene97699:intron 25.0%
!!! TTTTTTATTTGTCTTGTAGG+TGG + chr5.1:20454940-20454959 MS.gene97699:intron 25.0%
AAAATAGTCTCTAACTAGCT+AGG + chr5.1:20454195-20454214 MS.gene97699:intron 30.0%
AACCCTAGTTTATACTCTTA+TGG + chr5.1:20455389-20455408 MS.gene97699:CDS 30.0%
ATATTCCCTAAAGGGTTATA+AGG - chr5.1:20455269-20455288 None:intergenic 30.0%
ATCAAAGGTAAAGAAGAAAC+TGG - chr5.1:20454854-20454873 None:intergenic 30.0%
ATGAGATTGGTGTAAAGAAA+GGG + chr5.1:20454445-20454464 MS.gene97699:intron 30.0%
ATGAGTCTTTCAAACTTACT+TGG + chr5.1:20455101-20455120 MS.gene97699:CDS 30.0%
ATGTTCCAAGACAAAACTAT+AGG - chr5.1:20454381-20454400 None:intergenic 30.0%
CATAGGTTGCAATATTATGT+GGG - chr5.1:20454343-20454362 None:intergenic 30.0%
GTAAGTTTGAAAGACTCATA+AGG - chr5.1:20455100-20455119 None:intergenic 30.0%
GTATTTATACAATCCTAGGA+AGG + chr5.1:20453450-20453469 MS.gene97699:CDS 30.0%
TAAGGTGGACAAATTATTTG+AGG + chr5.1:20454586-20454605 MS.gene97699:intron 30.0%
TAGGGTTAATATTCCCTAAA+GGG - chr5.1:20455277-20455296 None:intergenic 30.0%
TATATCAACAAAAATGGCCA+TGG + chr5.1:20454504-20454523 MS.gene97699:intron 30.0%
TCATCAAAGATTGCATTTGA+AGG - chr5.1:20455617-20455636 None:intergenic 30.0%
TGATTGAAAATTCCAGTTCT+AGG - chr5.1:20455548-20455567 None:intergenic 30.0%
TGTGCAATATTATTATGGGT+AGG + chr5.1:20454409-20454428 MS.gene97699:intron 30.0%
TTACTTGGCATGTCAAATAT+AGG + chr5.1:20455116-20455135 MS.gene97699:CDS 30.0%
! ATATCTTGTTGTAAACCAAG+AGG - chr5.1:20455161-20455180 None:intergenic 30.0%
! CAAATGCAATCTTTGATGAT+TGG + chr5.1:20455618-20455637 MS.gene97699:CDS 30.0%
! GTCCATAAGAGTATAAACTA+GGG - chr5.1:20455394-20455413 None:intergenic 30.0%
! TAGCTAGTTAGAGACTATTT+TGG - chr5.1:20454196-20454215 None:intergenic 30.0%
! TATCTTGTTGTAAACCAAGA+GGG - chr5.1:20455160-20455179 None:intergenic 30.0%
! TCAAAGCTGGATATTTCTAA+AGG + chr5.1:20455425-20455444 MS.gene97699:CDS 30.0%
! TGTCCATAAGAGTATAAACT+AGG - chr5.1:20455395-20455414 None:intergenic 30.0%
!! AGGAGTATTTATACAATCCT+AGG + chr5.1:20453446-20453465 MS.gene97699:CDS 30.0%
AAAAATGGCCATGGAAATTG+GGG + chr5.1:20454513-20454532 MS.gene97699:intron 35.0%
AAGGAAAAAGCTTCTCAAGA+TGG + chr5.1:20455031-20455050 MS.gene97699:CDS 35.0%
AATGAGCCATTAAGTTTGAG+TGG + chr5.1:20455347-20455366 MS.gene97699:CDS 35.0%
AATTTCAGGAATCTTATGCC+AGG + chr5.1:20453568-20453587 MS.gene97699:intron 35.0%
AATTTCCCTCTCTTGATATC+AGG - chr5.1:20454612-20454631 None:intergenic 35.0%
ACAAAAATGGCCATGGAAAT+TGG + chr5.1:20454511-20454530 MS.gene97699:intron 35.0%
AGAAAATCTACTTCCTGCTT+TGG + chr5.1:20455511-20455530 MS.gene97699:CDS 35.0%
AGAAACTGGTCATCAAAAGT+TGG - chr5.1:20454840-20454859 None:intergenic 35.0%
AGGAAAAAGCTTCTCAAGAT+GGG + chr5.1:20455032-20455051 MS.gene97699:CDS 35.0%
AGGTGATTGTAATCAGTTCT+TGG - chr5.1:20455080-20455099 None:intergenic 35.0%
ATCAACCTTCATTCAGTACT+TGG + chr5.1:20454657-20454676 MS.gene97699:intron 35.0%
ATTTGAAGGTGAGTGATCAA+TGG - chr5.1:20455603-20455622 None:intergenic 35.0%
CAAAAATGGCCATGGAAATT+GGG + chr5.1:20454512-20454531 MS.gene97699:intron 35.0%
CTAGGGTTAATATTCCCTAA+AGG - chr5.1:20455278-20455297 None:intergenic 35.0%
GATGAGATTGGTGTAAAGAA+AGG + chr5.1:20454444-20454463 MS.gene97699:intron 35.0%
GCATAGGTTGCAATATTATG+TGG - chr5.1:20454344-20454363 None:intergenic 35.0%
GCTAGCATATATGGTTTGAA+AGG - chr5.1:20454039-20454058 None:intergenic 35.0%
GGTTATAGGAAACTTAGCAT+AGG - chr5.1:20454360-20454379 None:intergenic 35.0%
GTTGAGACGTTACAAATTTC+AGG + chr5.1:20453554-20453573 MS.gene97699:intron 35.0%
TACCTTGTATTGTTGTGAGA+AGG + chr5.1:20453426-20453445 MS.gene97699:CDS 35.0%
! AAGCTGGATATTTCTAAAGG+TGG + chr5.1:20455428-20455447 MS.gene97699:CDS 35.0%
! AGCACATTTTATGATGGTTC+TGG - chr5.1:20453662-20453681 None:intergenic 35.0%
! GCACATTTTATGATGGTTCT+GGG - chr5.1:20453661-20453680 None:intergenic 35.0%
!! ATTGGTCTTTGGGTAAAAGA+GGG + chr5.1:20453594-20453613 MS.gene97699:intron 35.0%
!! TGTTTCCAAGTACTGAATGA+AGG - chr5.1:20454665-20454684 None:intergenic 35.0%
AAAATTGCAGCTCATCTTCC+AGG + chr5.1:20454966-20454985 MS.gene97699:CDS 40.0%
ACAAAGTGAGTCTTCAAAGC+TGG + chr5.1:20455412-20455431 MS.gene97699:CDS 40.0%
ACACCAATCTCATCACAACA+TGG - chr5.1:20454438-20454457 None:intergenic 40.0%
ATGAACAAACGTACGTACGT+AGG - chr5.1:20453867-20453886 None:intergenic 40.0%
CTCCTTCTCACAACAATACA+AGG - chr5.1:20453431-20453450 None:intergenic 40.0%
GAAGTGTGTGCTAGCATATA+TGG - chr5.1:20454048-20454067 None:intergenic 40.0%
GTAGCAAAAACAGAACTGCA+TGG - chr5.1:20453892-20453911 None:intergenic 40.0%
TACGTACGTACGTTTGTTCA+TGG + chr5.1:20453866-20453885 MS.gene97699:intron 40.0%
TCACCACCACTCAAACTTAA+TGG - chr5.1:20455356-20455375 None:intergenic 40.0%
TCACCATGTTGTGATGAGAT+TGG + chr5.1:20454432-20454451 MS.gene97699:intron 40.0%
TCATCTTCTTCTTGTGTCCA+TGG - chr5.1:20454471-20454490 None:intergenic 40.0%
! AAAGTGTTCCCCAATTTCCA+TGG - chr5.1:20454524-20454543 None:intergenic 40.0%
! ACTTTTAGCAACAACCCTCT+TGG + chr5.1:20455143-20455162 MS.gene97699:CDS 40.0%
! CTCTAAAAGCTCTTTCCAGT+AGG - chr5.1:20455678-20455697 None:intergenic 40.0%
!! GAAAATTCCAGTTCTAGGAG+AGG - chr5.1:20455543-20455562 None:intergenic 40.0%
!! GATTGGTCTTTGGGTAAAAG+AGG + chr5.1:20453593-20453612 MS.gene97699:intron 40.0%
!!! ACAATTGCATTTTGGCTAGG+TGG - chr5.1:20455187-20455206 None:intergenic 40.0%
!!! CAATTGCATTTTGGCTAGGT+GGG - chr5.1:20455186-20455205 None:intergenic 40.0%
!!! GCAACAATTGCATTTTGGCT+AGG - chr5.1:20455190-20455209 None:intergenic 40.0%
ATAGGTGTGGGAAGAGTTGT+AGG + chr5.1:20454562-20454581 MS.gene97699:intron 45.0%
ATCTCGTTATCTGTTCGTCC+TGG - chr5.1:20454987-20455006 None:intergenic 45.0%
CACGTATGAGCTGACATTTG+AGG - chr5.1:20454241-20454260 None:intergenic 45.0%
GAATCTTATGCCAGGTGGAT+TGG + chr5.1:20453576-20453595 MS.gene97699:intron 45.0%
GAGCCATTAAGTTTGAGTGG+TGG + chr5.1:20455350-20455369 MS.gene97699:CDS 45.0%
GGAAGAGTTGTAGGTTAAGG+TGG + chr5.1:20454571-20454590 MS.gene97699:intron 45.0%
GTGGGAAGAGTTGTAGGTTA+AGG + chr5.1:20454568-20454587 MS.gene97699:intron 45.0%
TACGTACGTAGGATGTTGCA+GGG - chr5.1:20453856-20453875 None:intergenic 45.0%
TGAGGCCTGATATCAAGAGA+GGG + chr5.1:20454604-20454623 MS.gene97699:intron 45.0%
TTACAGACGACACCCTTCAA+TGG + chr5.1:20453497-20453516 MS.gene97699:CDS 45.0%
TTACGCTTCAACACCTTCCT+AGG - chr5.1:20453466-20453485 None:intergenic 45.0%
TTCAGGAATCTTATGCCAGG+TGG + chr5.1:20453571-20453590 MS.gene97699:intron 45.0%
TTCCTTGACGACGAATCAAG+TGG + chr5.1:20455647-20455666 MS.gene97699:CDS 45.0%
TTGAGGCCTGATATCAAGAG+AGG + chr5.1:20454603-20454622 MS.gene97699:intron 45.0%
TTGGTGTAAAGAAAGGGCCA+TGG + chr5.1:20454451-20454470 MS.gene97699:intron 45.0%
! AAAGAGGGTTGTGACTTGTG+AGG + chr5.1:20453609-20453628 MS.gene97699:intron 45.0%
!! AGGAAGGTGTTGAAGCGTAA+AGG + chr5.1:20453466-20453485 MS.gene97699:CDS 45.0%
!! CAAAGCGTGCTGGTTTGAAT+AGG + chr5.1:20454544-20454563 MS.gene97699:intron 45.0%
!! GGAAGGTGTTGAAGCGTAAA+GGG + chr5.1:20453467-20453486 MS.gene97699:CDS 45.0%
!! GTGCTGGTTTGAATAGGTGT+GGG + chr5.1:20454550-20454569 MS.gene97699:intron 45.0%
!! GTTCTTGGCTTGTGAGTTTC+AGG - chr5.1:20455065-20455084 None:intergenic 45.0%
AATCAAGTGGTGGCTCCTAC+TGG + chr5.1:20455660-20455679 MS.gene97699:CDS 50.0%
ACGAGTTCAATGCCCTCCAA+CGG - chr5.1:20453536-20453555 None:intergenic 50.0%
CTTGACGACGAATCAAGTGG+TGG + chr5.1:20455650-20455669 MS.gene97699:CDS 50.0%
GGAACACTTTCAAAGCGTGC+TGG + chr5.1:20454534-20454553 MS.gene97699:intron 50.0%
GTACGTACGTAGGATGTTGC+AGG - chr5.1:20453857-20453876 None:intergenic 50.0%
GTTGCTTCCTCTCCTAGAAC+TGG + chr5.1:20455533-20455552 MS.gene97699:CDS 50.0%
TACCCAAAGACCAATCCACC+TGG - chr5.1:20453589-20453608 None:intergenic 50.0%
TGCCAGAGCACACCATTGAA+GGG - chr5.1:20453512-20453531 None:intergenic 50.0%
TTGTGAGGTCGCAAGGGATT+GGG + chr5.1:20453624-20453643 MS.gene97699:intron 50.0%
! CACCACTTGATTCGTCGTCA+AGG - chr5.1:20455652-20455671 None:intergenic 50.0%
! TGTGACTTGTGAGGTCGCAA+GGG + chr5.1:20453618-20453637 MS.gene97699:intron 50.0%
! TTGTGACTTGTGAGGTCGCA+AGG + chr5.1:20453617-20453636 MS.gene97699:intron 50.0%
!! AGGTGTTGAAGCGTAAAGGG+AGG + chr5.1:20453470-20453489 MS.gene97699:CDS 50.0%
!! ATGCCAGGTGGATTGGTCTT+TGG + chr5.1:20453583-20453602 MS.gene97699:intron 50.0%
!! CGTGCTGGTTTGAATAGGTG+TGG + chr5.1:20454549-20454568 MS.gene97699:intron 50.0%
!! TGCCAGGTGGATTGGTCTTT+GGG + chr5.1:20453584-20453603 MS.gene97699:intron 50.0%
CACCCTTCAATGGTGTGCTC+TGG + chr5.1:20453507-20453526 MS.gene97699:intron 55.0%
CTGCCAGAGCACACCATTGA+AGG - chr5.1:20453513-20453532 None:intergenic 55.0%
CTTGTGAGGTCGCAAGGGAT+TGG + chr5.1:20453623-20453642 MS.gene97699:intron 55.0%
GGAGAGGAAGCAACCAAAGC+AGG - chr5.1:20455527-20455546 None:intergenic 55.0%
!! GATGGTTCTGGGCTAGTCCA+AGG - chr5.1:20453650-20453669 None:intergenic 55.0%
GGTCGCAAGGGATTGGGCCT+TGG + chr5.1:20453630-20453649 MS.gene97699:intron 65.0%
GTGCTCTGGCAGCCGTTGGA+GGG + chr5.1:20453521-20453540 MS.gene97699:intron 65.0%
TGGTGTGCTCTGGCAGCCGT+TGG + chr5.1:20453517-20453536 MS.gene97699:intron 65.0%
TGTGCTCTGGCAGCCGTTGG+AGG + chr5.1:20453520-20453539 MS.gene97699:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 20453412 20455700 20453412 ID=MS.gene97699
chr5.1 mRNA 20453412 20455700 20453412 ID=MS.gene97699.t1;Parent=MS.gene97699
chr5.1 exon 20453412 20453518 20453412 ID=MS.gene97699.t1.exon1;Parent=MS.gene97699.t1
chr5.1 CDS 20453412 20453518 20453412 ID=cds.MS.gene97699.t1;Parent=MS.gene97699.t1
chr5.1 exon 20454959 20455700 20454959 ID=MS.gene97699.t1.exon2;Parent=MS.gene97699.t1
chr5.1 CDS 20454959 20455700 20454959 ID=cds.MS.gene97699.t1;Parent=MS.gene97699.t1
Gene Sequence

>MS.gene97699

ATGGAGAAATTGTGTACCTTGTATTGTTGTGAGAAGGAGTATTTATACAATCCTAGGAAGGTGTTGAAGCGTAAAGGGAGGAACGTTACAGACGACACCCTTCAATGGTGTGCTCTGGCAGCCGTTGGAGGGCATTGAACTCGTTGAGACGTTACAAATTTCAGGAATCTTATGCCAGGTGGATTGGTCTTTGGGTAAAAGAGGGTTGTGACTTGTGAGGTCGCAAGGGATTGGGCCTTGGACTAGCCCAGAACCATCATAAAATGTGCTAAATATTGTTGATGATCCTGATCATAAAACAAAAAAGGTCTTTGATGATATAATTTAGATCGTCACAAACTTTTATTTAAAATTTAACGATAAACTAATTGAACAACTAGCATTATTTCAGAGTTAGAAATTTATTTAGTTTTCCTTTTCATAATTAAATTCTGCGTCACACCCTGCAACATCCTACGTACGTACGTTTGTTCATGGCCATGCAGTTCTGTTTTTGCTACTCTCATAATCATGCATTTCTTGATTGTATCGAAAATCTTCAAATAATGTTGAGGAAAACATTATTGGTCAAAGTCACAATATATTAATTAACTATAATGATGAAATTCAAACTAGCACTTTAAGCCTTTCAAACCATATATGCTAGCACACACTTCAACAATTTCAATGAACTTACCTTTATTTTTAATTAAACGACACATACACTACCTAATCCAGTTATACTAAATTTCATAGTAATATTTTTGTAACAAAATAAAATTCTCTCCTTAGATGAAAAATGCCAAAATAGTCTCTAACTAGCTAGGTTGCTTCTTTGACACTTCTTCCTCAAATGTCAGCTCATACGTGTTATTTTCTTGAAGAAAAAAATAATTAGCAAAAATGATGGCATACTTTGATTTTACATATCACACTACACCTATAAAATCCCACATAATATTGCAACCTATGCTAAGTTTCCTATAACCTATAGTTTTGTCTTGGAACATTAATTCACTGTGCAATATTATTATGGGTAGGTCACCATGTTGTGATGAGATTGGTGTAAAGAAAGGGCCATGGACACAAGAAGAAGATGAGAAGCTGATTGATTATATCAACAAAAATGGCCATGGAAATTGGGGAACACTTTCAAAGCGTGCTGGTTTGAATAGGTGTGGGAAGAGTTGTAGGTTAAGGTGGACAAATTATTTGAGGCCTGATATCAAGAGAGGGAAATTCACTGATGAAGAAGAAAGAGTTATCATCAACCTTCATTCAGTACTTGGAAACAAGTATATTTCTTAACTTGTTTTTTTTTTTTTTTTTTTTTTATGTAGAAAATGATATTGAAATCACTCTAATTCCCTTTTATACTAGAAAAAATTGAAATAGCGACGAAAAAAATAAATTCGTCTCTAATTCTATCACTAAGTGTTAGAGACGCCAACTTTTGATGACCAGTTTCTTCTTTACCTTTGATTTTTGTCAATATTTCAACTATTTTTAGTAGCTTCATTTATTCAAGAGCTATAAATATAATGTGATTTTTTTTATTTGTCTTGTAGGTGGTCGAAAATTGCAGCTCATCTTCCAGGACGAACAGATAACGAGATAAAGAATTATTGGAACACAAACATAAGGAAAAAGCTTCTCAAGATGGGAATTGATCCTGAAACTCACAAGCCAAGAACTGATTACAATCACCTTATGAGTCTTTCAAACTTACTTGGCATGTCAAATATAGGCAATACTTTTAGCAACAACCCTCTTGGTTTACAACAAGATATTACCCACCTAGCCAAAATGCAATTGTTGCAAAATATGTTGCAAATTATGAATACCAATAATTCATTTGGTAACTTGGGGAATTATCCTTATAACCCTTTAGGGAATATTAACCCTAGCTTTAATCCATTCAATTTGTTTTTGAATGGAACAAACACAATTCAAACTAATGAGCCATTAAGTTTGAGTGGTGGTGAAGAATATGCTATGAACCCTAGTTTATACTCTTATGGACAAAGTGAGTCTTCAAAGCTGGATATTTCTAAAGGTGGATCAAGTTTACAAGATCTTGATTATAGCAAAATGGGAAGCATTTCATGTGAAAATCAAGAAGAAAATCTACTTCCTGCTTTGGTTGCTTCCTCTCCTAGAACTGGAATTTTCAATCAAATGGAAAATGATTGTAACAAAGCTCAAATGTCCATTGATCACTCACCTTCAAATGCAATCTTTGATGATTGGGAGAAGTTCCTTGACGACGAATCAAGTGGTGGCTCCTACTGGAAAGAGCTTTTAGAGTAA
Protein sequence

>MS.gene97699.t1

MEKLCTLYCCEKEYLYNPRKVLKRKGRNVTDDTLQWWSKIAAHLPGRTDNEIKNYWNTNIRKKLLKMGIDPETHKPRTDYNHLMSLSNLLGMSNIGNTFSNNPLGLQQDITHLAKMQLLQNMLQIMNTNNSFGNLGNYPYNPLGNINPSFNPFNLFLNGTNTIQTNEPLSLSGGEEYAMNPSLYSYGQSESSKLDISKGGSSLQDLDYSKMGSISCENQEENLLPALVASSPRTGIFNQMENDCNKAQMSIDHSPSNAIFDDWEKFLDDESSGGSYWKELLE