Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97833.t1 | AES72521.1 | 49.8 | 215 | 66 | 3 | 12 | 223 | 26 | 201 | 3.80E-44 | 188.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97833.t1 | Q9FGD1 | 55.8 | 43 | 19 | 0 | 181 | 223 | 164 | 206 | 7.1e-07 | 55.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97833.t1 | G7J7G0 | 49.8 | 215 | 66 | 3 | 12 | 223 | 26 | 201 | 2.7e-44 | 188.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene97833.t1 | TF | RWP-RK |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97833.t1 | MTR_3g091700 | 56.221 | 217 | 53 | 4 | 12 | 225 | 26 | 203 | 1.29e-73 | 224 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 62 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTGCAATTTGATGATATA+AGG | 0.196215 | 5.4:+40491442 | MS.gene97833:CDS |
| TCAGTGTAATGCCCCATTTC+AGG | 0.244696 | 5.4:-40491138 | None:intergenic |
| TTCTTAGCAGCCAAGTTTAT+AGG | 0.272498 | 5.4:-40491480 | None:intergenic |
| AACTGCTTTCATCAAGGTTT+GGG | 0.318271 | 5.4:-40491080 | None:intergenic |
| TCACCATTTGATGATTTCAT+TGG | 0.329299 | 5.4:+40490973 | MS.gene97833:CDS |
| AAACTGCTTTCATCAAGGTT+TGG | 0.356625 | 5.4:-40491081 | None:intergenic |
| CACCATTTGATGATTTCATT+GGG | 0.365731 | 5.4:+40490974 | MS.gene97833:CDS |
| GCTTCCGATAAAACAGGAAA+TGG | 0.376610 | 5.4:+40491195 | MS.gene97833:CDS |
| CCTTGATGAAAGCAGTTTAG+AGG | 0.385174 | 5.4:+40491086 | MS.gene97833:CDS |
| CCCTGCATCTATTTCTCAAA+AGG | 0.394587 | 5.4:-40491521 | None:intergenic |
| GTTGAGGCTTCCGATAAAAC+AGG | 0.414081 | 5.4:+40491189 | MS.gene97833:CDS |
| ATGATTTCATTGGGCTTGAT+CGG | 0.415600 | 5.4:+40490983 | MS.gene97833:CDS |
| TTCAGGTAAAACAACATTGA+AGG | 0.447782 | 5.4:-40491121 | None:intergenic |
| TCATGTAAAGTTGTTTACTT+TGG | 0.451356 | 5.4:-40491023 | None:intergenic |
| TGATTTCATTGGGCTTGATC+GGG | 0.452084 | 5.4:+40490984 | MS.gene97833:CDS |
| CTGCTACTTGGCAATGGTAA+TGG | 0.469995 | 5.4:-40491381 | None:intergenic |
| CCTCTAAACTGCTTTCATCA+AGG | 0.476137 | 5.4:-40491086 | None:intergenic |
| ATTGATGAGAAAGTTGCAAT+TGG | 0.492508 | 5.4:+40490591 | None:intergenic |
| TTGTGAAGAAAGAGGTTGAA+GGG | 0.494240 | 5.4:+40491343 | MS.gene97833:CDS |
| GTACTAGTACTAGTATCAAA+AGG | 0.498409 | 5.4:+40491406 | MS.gene97833:CDS |
| GTACTAGTACTGCTGCTACT+TGG | 0.498432 | 5.4:-40491393 | None:intergenic |
| AGCCCAATGAAATCATCAAA+TGG | 0.508117 | 5.4:-40490976 | None:intergenic |
| GCTTCGGCGAACACTGAAAG+TGG | 0.511245 | 5.4:+40491309 | MS.gene97833:CDS |
| TTTGTGAAGAAAGAGGTTGA+AGG | 0.514546 | 5.4:+40491342 | MS.gene97833:CDS |
| CTTCCGATAAAACAGGAAAT+GGG | 0.515746 | 5.4:+40491196 | MS.gene97833:CDS |
| TCAGGTAAAACAACATTGAA+GGG | 0.518157 | 5.4:-40491120 | None:intergenic |
| TGCTTTCATCAAGGTTTGGG+AGG | 0.535946 | 5.4:-40491077 | None:intergenic |
| TGTGAAGAAAGAGGTTGAAG+GGG | 0.552274 | 5.4:+40491344 | MS.gene97833:CDS |
| CCTGCATCTATTTCTCAAAA+GGG | 0.564580 | 5.4:-40491520 | None:intergenic |
| CCTTATATCATCAAATTGCA+AGG | 0.565178 | 5.4:-40491442 | None:intergenic |
| GTACTAGTATCAAAAGGAAG+AGG | 0.566406 | 5.4:+40491412 | MS.gene97833:CDS |
| ACATCACTAGGTGGTCTCAC+AGG | 0.590689 | 5.4:+40491550 | MS.gene97833:CDS |
| GCTGCTAAGAAAATGAAAGT+TGG | 0.591961 | 5.4:+40491492 | MS.gene97833:CDS |
| AAAGGTAGTATTGCTGAGAG+TGG | 0.600909 | 5.4:+40491162 | MS.gene97833:CDS |
| CTTCGGCGAACACTGAAAGT+GGG | 0.613874 | 5.4:+40491310 | MS.gene97833:CDS |
| GCAGGGAGCTCAACATCACT+AGG | 0.620032 | 5.4:+40491538 | MS.gene97833:CDS |
| ACATTGAGTGCTGCGAAGAG+TGG | 0.631997 | 5.4:+40491276 | MS.gene97833:CDS |
| GTACTGCTGCTACTTGGCAA+TGG | 0.637810 | 5.4:-40491387 | None:intergenic |
| GCACTCAATGTCAATGACAA+TGG | 0.649619 | 5.4:-40491264 | None:intergenic |
| ATGGGGCATTACACTGACAA+AGG | 0.650100 | 5.4:+40491144 | MS.gene97833:CDS |
| GGGAGCTCAACATCACTAGG+TGG | 0.708524 | 5.4:+40491541 | MS.gene97833:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAATGAACATTAAATCA+TGG | - | chr5.4:40490667-40490686 | None:intergenic | 20.0% |
| !! | ATTCTGTACACATTAAAAAA+GGG | - | chr5.4:40490943-40490962 | None:intergenic | 20.0% |
| !! | TAATAGAATAGAATAGTCTT+CGG | + | chr5.4:40490831-40490850 | MS.gene97833:intron | 20.0% |
| !! | TTTATCTATAACTATGACTA+TGG | + | chr5.4:40490762-40490781 | MS.gene97833:intron | 20.0% |
| ! | GATTCTGTACACATTAAAAA+AGG | - | chr5.4:40490944-40490963 | None:intergenic | 25.0% |
| ! | TCATGTAAAGTTGTTTACTT+TGG | - | chr5.4:40491026-40491045 | None:intergenic | 25.0% |
| ! | TTCTGTACACATTAAAAAAG+GGG | - | chr5.4:40490942-40490961 | None:intergenic | 25.0% |
| !! | AGTTAGTTTGATGTAGATTA+AGG | - | chr5.4:40490717-40490736 | None:intergenic | 25.0% |
| !! | TTTTGATGTTCCTATAAACT+TGG | + | chr5.4:40491470-40491489 | MS.gene97833:CDS | 25.0% |
| !!! | AATGTTGTTTTACCTGAAAT+GGG | + | chr5.4:40491126-40491145 | MS.gene97833:CDS | 25.0% |
| CCTTATATCATCAAATTGCA+AGG | - | chr5.4:40491445-40491464 | None:intergenic | 30.0% | |
| GTACTAGTACTAGTATCAAA+AGG | + | chr5.4:40491406-40491425 | MS.gene97833:CDS | 30.0% | |
| TCAGGTAAAACAACATTGAA+GGG | - | chr5.4:40491123-40491142 | None:intergenic | 30.0% | |
| TCTGTACACATTAAAAAAGG+GGG | - | chr5.4:40490941-40490960 | None:intergenic | 30.0% | |
| TTCAGGTAAAACAACATTGA+AGG | - | chr5.4:40491124-40491143 | None:intergenic | 30.0% | |
| ! | AAACCCATTTCCTGTTTTAT+CGG | - | chr5.4:40491202-40491221 | None:intergenic | 30.0% |
| ! | CACCATTTGATGATTTCATT+GGG | + | chr5.4:40490974-40490993 | MS.gene97833:CDS | 30.0% |
| ! | CCTTGCAATTTGATGATATA+AGG | + | chr5.4:40491442-40491461 | MS.gene97833:CDS | 30.0% |
| ! | TCACCATTTGATGATTTCAT+TGG | + | chr5.4:40490973-40490992 | MS.gene97833:CDS | 30.0% |
| !!! | ATGTTGTTTTACCTGAAATG+GGG | + | chr5.4:40491127-40491146 | MS.gene97833:CDS | 30.0% |
| !!! | CAATGTTGTTTTACCTGAAA+TGG | + | chr5.4:40491125-40491144 | MS.gene97833:CDS | 30.0% |
| AAACTGCTTTCATCAAGGTT+TGG | - | chr5.4:40491084-40491103 | None:intergenic | 35.0% | |
| AACTGCTTTCATCAAGGTTT+GGG | - | chr5.4:40491083-40491102 | None:intergenic | 35.0% | |
| AGCCCAATGAAATCATCAAA+TGG | - | chr5.4:40490979-40490998 | None:intergenic | 35.0% | |
| ATGATTTCATTGGGCTTGAT+CGG | + | chr5.4:40490983-40491002 | MS.gene97833:CDS | 35.0% | |
| CACATTAAAAAAGGGGGAAA+CGG | - | chr5.4:40490935-40490954 | None:intergenic | 35.0% | |
| CTTCCGATAAAACAGGAAAT+GGG | + | chr5.4:40491196-40491215 | MS.gene97833:CDS | 35.0% | |
| GCTGCTAAGAAAATGAAAGT+TGG | + | chr5.4:40491492-40491511 | MS.gene97833:CDS | 35.0% | |
| GTACTAGTATCAAAAGGAAG+AGG | + | chr5.4:40491412-40491431 | MS.gene97833:CDS | 35.0% | |
| TTCTTAGCAGCCAAGTTTAT+AGG | - | chr5.4:40491483-40491502 | None:intergenic | 35.0% | |
| TTGTGAAGAAAGAGGTTGAA+GGG | + | chr5.4:40491343-40491362 | MS.gene97833:CDS | 35.0% | |
| TTTGTGAAGAAAGAGGTTGA+AGG | + | chr5.4:40491342-40491361 | MS.gene97833:CDS | 35.0% | |
| ! | CATTCTCATTACGACTTTTG+AGG | + | chr5.4:40490870-40490889 | MS.gene97833:intron | 35.0% |
| ! | CCTGCATCTATTTCTCAAAA+GGG | - | chr5.4:40491523-40491542 | None:intergenic | 35.0% |
| !! | CCTTTTGAGAAATAGATGCA+GGG | + | chr5.4:40491521-40491540 | MS.gene97833:CDS | 35.0% |
| !! | TGTGAGTTTTGTGAAGAAAG+AGG | + | chr5.4:40491221-40491240 | MS.gene97833:CDS | 35.0% |
| !!! | TACGACTTTTGAGGTTGATA+TGG | + | chr5.4:40490879-40490898 | MS.gene97833:intron | 35.0% |
| AAAGGTAGTATTGCTGAGAG+TGG | + | chr5.4:40491162-40491181 | MS.gene97833:CDS | 40.0% | |
| CCTCTAAACTGCTTTCATCA+AGG | - | chr5.4:40491089-40491108 | None:intergenic | 40.0% | |
| CCTTGATGAAAGCAGTTTAG+AGG | + | chr5.4:40491086-40491105 | MS.gene97833:CDS | 40.0% | |
| GCACTCAATGTCAATGACAA+TGG | - | chr5.4:40491267-40491286 | None:intergenic | 40.0% | |
| GCTTCCGATAAAACAGGAAA+TGG | + | chr5.4:40491195-40491214 | MS.gene97833:CDS | 40.0% | |
| TGATTTCATTGGGCTTGATC+GGG | + | chr5.4:40490984-40491003 | MS.gene97833:CDS | 40.0% | |
| TGTGAAGAAAGAGGTTGAAG+GGG | + | chr5.4:40491344-40491363 | MS.gene97833:CDS | 40.0% | |
| ! | CCCTGCATCTATTTCTCAAA+AGG | - | chr5.4:40491524-40491543 | None:intergenic | 40.0% |
| !! | CCCTTTTGAGAAATAGATGC+AGG | + | chr5.4:40491520-40491539 | MS.gene97833:CDS | 40.0% |
| AGTCTTCGGAGAATGATGTG+TGG | + | chr5.4:40490845-40490864 | MS.gene97833:intron | 45.0% | |
| GTACTAGTACTGCTGCTACT+TGG | - | chr5.4:40491396-40491415 | None:intergenic | 45.0% | |
| GTTGAGGCTTCCGATAAAAC+AGG | + | chr5.4:40491189-40491208 | MS.gene97833:CDS | 45.0% | |
| TCAGTGTAATGCCCCATTTC+AGG | - | chr5.4:40491141-40491160 | None:intergenic | 45.0% | |
| TGCTTTCATCAAGGTTTGGG+AGG | - | chr5.4:40491080-40491099 | None:intergenic | 45.0% | |
| ! | ATGGGGCATTACACTGACAA+AGG | + | chr5.4:40491144-40491163 | MS.gene97833:CDS | 45.0% |
| ! | CTGCTACTTGGCAATGGTAA+TGG | - | chr5.4:40491384-40491403 | None:intergenic | 45.0% |
| !!! | GAGTGGTTTTGTCGAAGCTT+CGG | + | chr5.4:40491293-40491312 | MS.gene97833:CDS | 45.0% |
| !!! | TGCTGAGAGTGGTTTTGTTG+AGG | + | chr5.4:40491173-40491192 | MS.gene97833:CDS | 45.0% |
| ACATCACTAGGTGGTCTCAC+AGG | + | chr5.4:40491550-40491569 | MS.gene97833:CDS | 50.0% | |
| CTTCGGCGAACACTGAAAGT+GGG | + | chr5.4:40491310-40491329 | MS.gene97833:CDS | 50.0% | |
| ! | ACATTGAGTGCTGCGAAGAG+TGG | + | chr5.4:40491276-40491295 | MS.gene97833:CDS | 50.0% |
| ! | GTACTGCTGCTACTTGGCAA+TGG | - | chr5.4:40491390-40491409 | None:intergenic | 50.0% |
| GCAGGGAGCTCAACATCACT+AGG | + | chr5.4:40491538-40491557 | MS.gene97833:CDS | 55.0% | |
| GCTTCGGCGAACACTGAAAG+TGG | + | chr5.4:40491309-40491328 | MS.gene97833:CDS | 55.0% | |
| GGGAGCTCAACATCACTAGG+TGG | + | chr5.4:40491541-40491560 | MS.gene97833:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 40490595 | 40491599 | 40490595 | ID=MS.gene97833 |
| chr5.4 | mRNA | 40490595 | 40491599 | 40490595 | ID=MS.gene97833.t1;Parent=MS.gene97833 |
| chr5.4 | exon | 40490595 | 40490635 | 40490595 | ID=MS.gene97833.t1.exon1;Parent=MS.gene97833.t1 |
| chr5.4 | CDS | 40490595 | 40490635 | 40490595 | ID=cds.MS.gene97833.t1;Parent=MS.gene97833.t1 |
| chr5.4 | exon | 40490960 | 40491599 | 40490960 | ID=MS.gene97833.t1.exon2;Parent=MS.gene97833.t1 |
| chr5.4 | CDS | 40490960 | 40491599 | 40490960 | ID=cds.MS.gene97833.t1;Parent=MS.gene97833.t1 |
| Gene Sequence |
| Protein sequence |
