AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene97979

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97979.t1 MTR_8g070075 83.377 764 78 2 1 721 1 758 0.0 1309
MS.gene97979.t1 MTR_8g070070 68.917 785 165 11 4 725 1 769 0.0 1036
MS.gene97979.t1 MTR_8g071030 59.497 795 203 11 4 725 1 749 0.0 891
MS.gene97979.t1 MTR_1g035420 56.250 592 210 9 148 724 101 658 0.0 652
MS.gene97979.t1 MTR_1g035400 52.996 534 230 6 186 716 53 568 0.0 545
MS.gene97979.t1 MTR_6g044690 47.635 592 233 15 160 713 12 564 7.32e-167 492
MS.gene97979.t1 MTR_0376s0030 52.174 437 184 7 299 724 155 577 3.81e-152 454
MS.gene97979.t1 MTR_6g043960 46.169 522 230 12 203 713 1 482 3.58e-140 421
MS.gene97979.t1 MTR_2g054460 42.441 549 197 15 196 713 4 464 3.97e-122 373
MS.gene97979.t1 MTR_7g088370 42.005 419 208 12 305 713 287 680 1.52e-93 306
MS.gene97979.t1 MTR_5g013420 41.063 414 221 8 302 711 664 1058 1.41e-92 311
MS.gene97979.t1 MTR_5g013420 41.063 414 221 8 302 711 664 1058 1.81e-92 311
MS.gene97979.t1 MTR_4g010830 42.373 413 216 8 302 711 556 949 8.36e-92 308
MS.gene97979.t1 MTR_3g011440 40.426 423 223 12 305 712 225 633 3.09e-89 293
MS.gene97979.t1 MTR_8g042750 31.025 722 367 23 45 713 15 658 8.44e-86 285
MS.gene97979.t1 MTR_6g061270 38.967 426 232 13 306 712 248 664 3.36e-80 270
MS.gene97979.t1 MTR_7g084090 38.372 430 230 12 306 712 310 727 5.41e-80 271
MS.gene97979.t1 MTR_4g010550 38.499 413 202 11 302 711 70 433 9.01e-77 254
MS.gene97979.t1 MTR_6g061200 38.186 419 216 15 306 712 248 635 1.06e-74 254
MS.gene97979.t1 MTR_7g084090 41.748 309 161 10 306 602 310 611 2.90e-62 221
MS.gene97979.t1 MTR_7g052480 36.154 260 153 7 463 712 270 526 5.98e-43 164
MS.gene97979.t1 MTR_7g445530 31.807 415 194 17 317 711 167 512 2.56e-41 158
MS.gene97979.t1 MTR_7g445710 31.807 415 194 17 317 711 167 512 2.77e-41 159
MS.gene97979.t1 MTR_1g048950 32.075 424 236 12 305 711 119 507 6.09e-41 158
MS.gene97979.t1 MTR_7g450730 29.808 416 182 14 307 711 158 474 1.94e-39 153
MS.gene97979.t1 MTR_8g078505 27.915 283 157 9 460 711 249 515 8.48e-22 100
MS.gene97979.t1 MTR_7g445600 34.000 150 62 2 562 711 243 355 2.24e-21 97.1
MS.gene97979.t1 MTR_1g069570 26.710 307 153 12 463 713 430 720 1.90e-20 97.1
MS.gene97979.t1 MTR_6g044000 71.698 53 15 0 661 713 117 169 2.76e-20 89.4
MS.gene97979.t1 MTR_8g078490 31.217 189 110 4 528 711 1056 1229 4.81e-20 96.3
MS.gene97979.t1 MTR_4g132610 30.270 185 115 4 529 711 851 1023 5.36e-19 92.4
MS.gene97979.t1 MTR_5g018850 30.851 188 112 4 528 711 1310 1483 6.39e-19 92.4
MS.gene97979.t1 MTR_1g080340 25.407 307 158 11 463 713 269 560 2.40e-18 89.7
MS.gene97979.t1 MTR_1g069570 26.996 263 121 11 463 670 430 676 5.22e-17 85.9
MS.gene97979.t1 MTR_7g098390 25.080 311 159 12 459 713 384 676 9.71e-16 81.6
MS.gene97979.t1 MTR_5g098680 36.486 148 80 5 297 433 271 415 1.28e-15 81.3
MS.gene97979.t1 MTR_8g102580 36.486 148 80 5 297 433 265 409 4.25e-15 79.7
MS.gene97979.t1 MTR_3g114910 38.393 112 62 2 322 433 221 325 8.73e-14 74.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene97979.t1 AT4G13460 53.393 560 206 10 183 724 127 649 0.0 583
MS.gene97979.t1 AT4G13460 53.393 560 206 10 183 724 127 649 0.0 583
MS.gene97979.t1 AT2G33290 47.393 633 290 13 106 724 47 650 0.0 571
MS.gene97979.t1 AT2G33290 47.393 633 290 13 106 724 47 650 0.0 571
MS.gene97979.t1 AT2G33290 47.393 633 290 13 106 724 47 650 0.0 571
MS.gene97979.t1 AT2G33290 47.393 633 290 13 106 724 47 650 0.0 571
MS.gene97979.t1 AT5G04940 38.876 427 218 11 305 713 239 640 5.12e-84 280
MS.gene97979.t1 AT5G04940 38.876 427 218 11 305 713 239 640 5.12e-84 280
MS.gene97979.t1 AT2G22740 39.089 417 231 8 304 711 357 759 1.82e-82 278
MS.gene97979.t1 AT2G22740 39.089 417 231 8 304 711 357 759 1.82e-82 278
MS.gene97979.t1 AT2G35160 40.291 412 202 10 304 711 356 727 4.04e-82 277
MS.gene97979.t1 AT2G35160 40.291 412 202 10 304 711 392 763 1.10e-81 276
MS.gene97979.t1 AT2G35160 40.291 412 202 10 304 711 392 763 1.10e-81 276
MS.gene97979.t1 AT2G35160 40.291 412 202 10 304 711 392 763 1.10e-81 276
MS.gene97979.t1 AT2G35160 40.291 412 202 10 304 711 392 763 1.10e-81 276
MS.gene97979.t1 AT1G73100 36.977 430 230 12 302 713 233 639 1.16e-79 268
MS.gene97979.t1 AT5G13960 37.939 427 235 14 306 712 44 460 2.03e-77 257
MS.gene97979.t1 AT5G13960 37.705 427 236 14 306 712 178 594 5.40e-77 260
MS.gene97979.t1 AT1G17770 32.618 466 258 18 283 713 217 661 6.05e-61 218
MS.gene97979.t1 AT2G24740 35.154 421 227 16 305 713 338 724 2.49e-56 206
MS.gene97979.t1 AT2G23740 26.829 369 196 15 412 723 1012 1363 8.98e-24 108
MS.gene97979.t1 AT2G23740 26.829 369 196 15 412 723 1019 1370 9.39e-24 108
MS.gene97979.t1 AT2G23740 26.829 369 196 15 412 723 1019 1370 9.39e-24 108
MS.gene97979.t1 AT2G23740 26.829 369 196 15 412 723 1019 1370 9.39e-24 108
MS.gene97979.t1 AT2G05900 38.202 178 102 6 318 495 26 195 4.09e-21 95.5
MS.gene97979.t1 AT5G47150 42.188 128 66 3 306 433 205 324 2.41e-19 90.5
MS.gene97979.t1 AT2G23740 26.518 313 159 14 412 670 1019 1314 8.42e-18 89.0
MS.gene97979.t1 AT2G23740 26.518 313 159 14 412 670 1019 1314 8.42e-18 89.0
MS.gene97979.t1 AT5G47160 43.411 129 64 4 303 430 288 408 5.30e-17 84.3
MS.gene97979.t1 AT5G47160 43.411 129 64 4 303 430 288 408 5.30e-17 84.3
MS.gene97979.t1 AT5G47160 43.411 129 64 4 303 430 288 408 5.30e-17 84.3
MS.gene97979.t1 AT3G03750 30.918 207 115 6 508 712 143 323 1.08e-16 82.8
MS.gene97979.t1 AT1G57820 40.678 118 62 3 322 433 311 426 1.84e-16 84.0
MS.gene97979.t1 AT1G57820 40.678 118 62 3 322 433 311 426 1.84e-16 84.0
MS.gene97979.t1 AT1G57820 40.000 115 64 3 322 433 311 423 1.79e-15 80.9
MS.gene97979.t1 AT1G66040 36.232 138 77 5 304 433 277 411 1.05e-14 78.2
MS.gene97979.t1 AT1G66050 36.232 138 77 5 304 433 277 411 1.14e-14 78.2
MS.gene97979.t1 AT5G39550 38.136 118 65 3 322 433 296 411 2.57e-14 77.0
MS.gene97979.t1 AT1G04050 24.919 309 154 10 463 713 409 697 5.10e-14 76.3
MS.gene97979.t1 AT1G04050 24.919 309 154 10 463 713 409 697 5.10e-14 76.3
MS.gene97979.t1 AT1G04050 24.595 309 155 10 463 713 305 593 6.02e-14 75.9
MS.gene97979.t1 AT4G08590 32.895 152 87 5 285 433 238 377 2.95e-12 70.1
MS.gene97979.t1 AT4G08590 32.237 152 89 5 285 433 238 378 1.61e-11 67.4
MS.gene97979.t1 AT4G08590 32.237 152 89 5 285 433 238 378 1.78e-11 67.4
MS.gene97979.t1 AT4G08590 32.237 152 89 5 285 433 238 378 2.42e-11 66.6

Find 205 sgRNAs with CRISPR-Local

Find 239 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTACTAGTGTGATTGTTTC+AGG 0.175344 8.4:+33037348 MS.gene97979:CDS
GCTAGGAAGATTAAGAAAAT+TGG 0.178663 8.4:+33037714 MS.gene97979:CDS
CATCAAAATGATTAGGTTTA+GGG 0.226810 8.4:-33036410 None:intergenic
ACTAAATCATAACCTTAATA+TGG 0.228635 8.4:+33036683 MS.gene97979:CDS
AGCTTGCCCGCCCACTCGTC+TGG 0.233131 8.4:-33038524 None:intergenic
TCATCAAAATGATTAGGTTT+AGG 0.248789 8.4:-33036411 None:intergenic
ACTCGTTAATATATCCAAAA+AGG 0.268007 8.4:+33038231 MS.gene97979:CDS
TGTTTCTGAAGTTGCGATTA+TGG 0.269601 8.4:+33037025 MS.gene97979:CDS
TGATTCTTGTTTATTTGAAA+TGG 0.272590 8.4:-33036748 None:intergenic
CAACAACCTCATTTCCCTTC+TGG 0.282693 8.4:+33036459 MS.gene97979:CDS
AAGCAAACCCTGAAGATTAT+AGG 0.291078 8.4:-33037976 None:intergenic
AATTATTGAGGAGAAGAAAT+TGG 0.298748 8.4:+33036932 MS.gene97979:CDS
TGAGAAGGGGAGGTAATAGA+AGG 0.299425 8.4:-33036297 None:intergenic
AGAGTTTCCCTATAATCTTC+AGG 0.299770 8.4:+33037968 MS.gene97979:CDS
GTTAATGATAAAGGATCTAT+TGG 0.313384 8.4:-33036264 None:intergenic
AGTAGCTCTGGTTAAGAAAA+AGG 0.316965 8.4:+33036779 MS.gene97979:CDS
ATGTCATTAAATAGCCTAAT+AGG 0.327262 8.4:-33037798 None:intergenic
AAGCGGTGCTCCGGTGGAAA+TGG 0.334829 8.4:-33036373 None:intergenic
GGATTGAGGGTTGACATGTT+AGG 0.336861 8.4:-33036339 None:intergenic
AGCAACACGATTCCGACAAT+TGG 0.337163 8.4:-33038051 None:intergenic
AGTGTAGAGGTTAGATTGAT+TGG 0.337421 8.4:-33036529 None:intergenic
ATACATTTGGACTCGAACTA+TGG 0.339033 8.4:-33038391 None:intergenic
TTGGGAGGACAAGAGCAAAA+AGG 0.339571 8.4:-33038032 None:intergenic
CACAAACTGGTTGGGGAGTT+AGG 0.342051 8.4:+33038114 MS.gene97979:CDS
GGTGGAAATGGGAGTGAGTT+GGG 0.348906 8.4:-33036361 None:intergenic
AAGAAATCATCAAAATGATT+AGG 0.351239 8.4:-33036417 None:intergenic
CAATGCTTCTGCAGCTTCAA+TGG 0.352411 8.4:+33036632 MS.gene97979:CDS
ATCATATTCGTGGTGACATA+TGG 0.355996 8.4:+33037135 MS.gene97979:CDS
AGGCGGTTATGAGGATGATA+TGG 0.357241 8.4:+33037368 MS.gene97979:CDS
TGTATAGGATTATTGATTGC+TGG 0.360049 8.4:+33037598 MS.gene97979:CDS
AGCATAGGTTGGAAGTGTTT+AGG 0.365235 8.4:+33038087 MS.gene97979:CDS
GTTTCAAGGGTTAATGATAA+AGG 0.365643 8.4:-33036273 None:intergenic
ACCTGATATAGATTATTCTA+TGG 0.365689 8.4:+33038331 MS.gene97979:CDS
GCATTACGGGATCGAGATTA+GGG 0.365857 8.4:+33037509 MS.gene97979:CDS
CTAGGAAGATTAAGAAAATT+GGG 0.368395 8.4:+33037715 MS.gene97979:CDS
TCCTGTATACTCGCAGATAA+AGG 0.370071 8.4:-33038162 None:intergenic
AGCAAACCCTGAAGATTATA+GGG 0.370075 8.4:-33037975 None:intergenic
AAACTCTCCTCCATTCTTCC+TGG 0.373456 8.4:-33037952 None:intergenic
TCCATAGAATAATCTATATC+AGG 0.373576 8.4:-33038332 None:intergenic
TGGGAGTGAGTTGGGGATTG+AGG 0.377704 8.4:-33036353 None:intergenic
ATTAGGTTTAGGGACGAGTA+TGG 0.379859 8.4:-33036400 None:intergenic
GATTGAGGGTTGACATGTTA+GGG 0.381292 8.4:-33036338 None:intergenic
TATAGATAGATGGGCAGAAT+GGG 0.382516 8.4:+33038256 MS.gene97979:CDS
TAATCTATATCAGGTATTAA+TGG 0.385147 8.4:-33038323 None:intergenic
TAGGTCTGCACAAACTGGTT+GGG 0.389899 8.4:+33038106 MS.gene97979:CDS
TTTATCGGAAGTAGCATTAG+AGG 0.389943 8.4:-33036574 None:intergenic
TGCATTACGGGATCGAGATT+AGG 0.391240 8.4:+33037508 MS.gene97979:CDS
TCGGAATCGTGTTGCTCAAA+AGG 0.393717 8.4:+33038058 MS.gene97979:CDS
TGAAGCTGCAGAAGCATTGT+TGG 0.393720 8.4:-33036628 None:intergenic
AGAGGGAGAATGTTCCTATT+AGG 0.395044 8.4:+33037784 MS.gene97979:CDS
AATTCTTCTTTCTTCCTCCT+TGG 0.396457 8.4:-33037425 None:intergenic
ACAAACTGAACAAATACATT+TGG 0.398498 8.4:-33038404 None:intergenic
CTAAATCATAACCTTAATAT+GGG 0.399163 8.4:+33036684 MS.gene97979:CDS
AGGTTGTTGTTGGGAACCTT+GGG 0.401571 8.4:-33036445 None:intergenic
TAAAGGCTCCAGCTTGAATA+AGG 0.402182 8.4:-33038145 None:intergenic
GCTCTTGTCCTCCCAATTGT+CGG 0.405346 8.4:+33038039 MS.gene97979:CDS
GATTCTTGTTTATTTGAAAT+GGG 0.406495 8.4:-33036747 None:intergenic
TAAAGGATCTATTGGATTCA+TGG 0.409301 8.4:-33036256 None:intergenic
TATAAGTTATTGAGAGTTGA+AGG 0.409965 8.4:+33037657 MS.gene97979:CDS
GGTAAGGTTTATGTTTATGA+TGG 0.410667 8.4:+33037573 MS.gene97979:CDS
CTGATCACAAATTTGAATTG+AGG 0.414878 8.4:-33037448 None:intergenic
AGAGATTGGTTGAGGAGAAG+AGG 0.415274 8.4:+33036982 MS.gene97979:CDS
GGGAGTGAGTTGGGGATTGA+GGG 0.418786 8.4:-33036352 None:intergenic
TTATGAGGATGATATGGAAT+TGG 0.420516 8.4:+33037374 MS.gene97979:CDS
AAAATGATAAAGGGATTTAT+CGG 0.421885 8.4:-33036589 None:intergenic
ATGTTTATGATGGATTGTAT+AGG 0.423249 8.4:+33037583 MS.gene97979:CDS
GCCTTTATCTGCGAGTATAC+AGG 0.424211 8.4:+33038161 MS.gene97979:CDS
TGTGGAGGCAGATAAGAGAT+TGG 0.428163 8.4:+33036968 MS.gene97979:CDS
GGGTTGACATGTTAGGGATT+TGG 0.431533 8.4:-33036332 None:intergenic
GGTGAGAAGAAGTTTGAGAA+GGG 0.432588 8.4:-33036311 None:intergenic
TCATATTCGTGGTGACATAT+GGG 0.435054 8.4:+33037136 MS.gene97979:CDS
CGGAATCGTGTTGCTCAAAA+GGG 0.437967 8.4:+33038059 MS.gene97979:CDS
GTGTTTAGGTCTGCACAAAC+TGG 0.452666 8.4:+33038101 MS.gene97979:CDS
AAGGGAAATGAGGTTGTTGT+TGG 0.453858 8.4:-33036455 None:intergenic
TCAACAGAAAGACAATTCAT+AGG 0.456778 8.4:-33037750 None:intergenic
TGCGATTATGGAGGCGGAGA+TGG 0.457729 8.4:+33037037 MS.gene97979:CDS
GGCAGATAAGAGATTGGTTG+AGG 0.458910 8.4:+33036974 MS.gene97979:CDS
GATGATATGGAATTGGATAA+TGG 0.459588 8.4:+33037381 MS.gene97979:CDS
CATCAGAGCGGGAAAGCTAC+TGG 0.462434 8.4:+33037888 MS.gene97979:CDS
GAGTTTCCCTATAATCTTCA+GGG 0.462595 8.4:+33037969 MS.gene97979:CDS
AGGTAATAGAAGGGGTTTCA+AGG 0.463004 8.4:-33036287 None:intergenic
CTTCTTTCTTCCTCCTTGGC+CGG 0.463064 8.4:-33037421 None:intergenic
AATCAATCTCATTTCCCTCC+TGG 0.464661 8.4:+33036495 MS.gene97979:CDS
ATTGATTGCTGGTTTGAAGA+AGG 0.466360 8.4:+33037609 MS.gene97979:CDS
ATCATGTTCGGCAGTAGCTC+TGG 0.471165 8.4:+33036767 MS.gene97979:CDS
ACAATCACACTAGTAGCAAT+TGG 0.471909 8.4:-33037342 None:intergenic
TCCTTGGACCTTATTCAAGC+TGG 0.472407 8.4:+33038137 MS.gene97979:CDS
AGTTGAAGGGCAAACTAAGA+TGG 0.474128 8.4:+33037671 MS.gene97979:CDS
GCTTGCCCGCCCACTCGTCT+GGG 0.475920 8.4:-33038523 None:intergenic
TTAGAGCGTAGTATGCATTA+CGG 0.480635 8.4:+33037495 MS.gene97979:CDS
TAAGGTTATGATTTAGTCCA+AGG 0.481584 8.4:-33036677 None:intergenic
TCAGTCAATATTTCTGACCT+TGG 0.482765 8.4:+33036660 MS.gene97979:CDS
GCAACACGATTCCGACAATT+GGG 0.484911 8.4:-33038050 None:intergenic
CTACTTAGTTACATACATAC+TGG 0.485115 8.4:-33038548 None:intergenic
TAGAGCGTAGTATGCATTAC+GGG 0.486228 8.4:+33037496 MS.gene97979:CDS
GAAGATTAAGAAAATTGGGT+TGG 0.487319 8.4:+33037719 MS.gene97979:CDS
TCAGGTATTAATGGATATGA+TGG 0.489556 8.4:-33038314 None:intergenic
AGCGGTGCTCCGGTGGAAAT+GGG 0.492091 8.4:-33036372 None:intergenic
AGGGAAATGAGGTTGTTGTT+GGG 0.492139 8.4:-33036454 None:intergenic
AGACGTTATAATCATATTCG+TGG 0.492546 8.4:+33037125 MS.gene97979:CDS
GGATGAGAAAAGAATTATTG+AGG 0.492648 8.4:+33036920 MS.gene97979:CDS
TTGATTGCTGGTTTGAAGAA+GGG 0.494752 8.4:+33037610 MS.gene97979:CDS
CTTCAGGGTTTGCTTGTGAA+AGG 0.494789 8.4:+33037984 MS.gene97979:CDS
TGATTGGTGTGTTCATATCC+AGG 0.498306 8.4:-33036513 None:intergenic
TATGGTTTGAAGCGGTGCTC+CGG 0.502918 8.4:-33036382 None:intergenic
TTGTTGTTGGGAACCTTGGG+TGG 0.503224 8.4:-33036442 None:intergenic
TGTGTGGAGGCTTGTACTGA+TGG 0.507598 8.4:+33037918 MS.gene97979:CDS
AGGTCTGCACAAACTGGTTG+GGG 0.507788 8.4:+33038107 MS.gene97979:CDS
GTGAGAAGAAGTTTGAGAAG+GGG 0.507936 8.4:-33036310 None:intergenic
AAATAAACAAGAATCATGTT+CGG 0.510967 8.4:+33036755 MS.gene97979:CDS
TAGATAGATGGGCAGAATGG+GGG 0.511424 8.4:+33038258 MS.gene97979:CDS
GCGATTATGGAGGCGGAGAT+GGG 0.511508 8.4:+33037038 MS.gene97979:CDS
ACATCAGATTGTTATGATCA+TGG 0.511816 8.4:-33038430 None:intergenic
AGAGAACTCAGCATAGACTA+TGG 0.515257 8.4:+33038497 MS.gene97979:CDS
GAGGTTGTTGTTGGGAACCT+TGG 0.515342 8.4:-33036446 None:intergenic
AGAAGAAGTTTGAGAAGGGG+AGG 0.515562 8.4:-33036307 None:intergenic
TGGTGAGAAGAAGTTTGAGA+AGG 0.516679 8.4:-33036312 None:intergenic
TTATAGATAGATGGGCAGAA+TGG 0.518603 8.4:+33038255 MS.gene97979:CDS
ATGGAGGCGGAGATGGGAGC+TGG 0.518912 8.4:+33037044 MS.gene97979:CDS
GATGATTTCTTCACCACCCA+AGG 0.524018 8.4:+33036429 MS.gene97979:CDS
CGGTGGAAATGGGAGTGAGT+TGG 0.525640 8.4:-33036362 None:intergenic
GGCATCCCAGACGAGTGGGC+GGG 0.530990 8.4:+33038518 MS.gene97979:CDS
AGATAGATGGGCAGAATGGG+GGG 0.535500 8.4:+33038259 MS.gene97979:CDS
GAGAAGGGGAGGTAATAGAA+GGG 0.535917 8.4:-33036296 None:intergenic
TTCGTGGGATGAAGTATGAA+GGG 0.537787 8.4:+33037535 MS.gene97979:CDS
TGGTTTGAAGAAGGGATGAA+TGG 0.538003 8.4:+33037618 MS.gene97979:CDS
TGAAGTTGCGATTATGGAGG+CGG 0.538130 8.4:+33037031 MS.gene97979:CDS
GGTGATGTAATAATCTATAC+CGG 0.538669 8.4:+33037402 MS.gene97979:CDS
AATTTGTGATCAGAAATTAG+AGG 0.546612 8.4:+33037458 MS.gene97979:CDS
AAAGGGGATCAAGCATAGGT+TGG 0.547811 8.4:+33038076 MS.gene97979:CDS
ATAAGTTATTGAGAGTTGAA+GGG 0.555115 8.4:+33037658 MS.gene97979:CDS
GATTGTTTCAGGCGGTTATG+AGG 0.556692 8.4:+33037359 MS.gene97979:CDS
GGTTTGTATTTCATCAGAGC+GGG 0.557067 8.4:+33037877 MS.gene97979:CDS
CAAATATGAGTATGAAGGAT+CGG 0.557193 8.4:+33036826 MS.gene97979:CDS
ATCGAGATTAGGGTTATTCG+TGG 0.558682 8.4:+33037519 MS.gene97979:CDS
AGCTACTGGCTGTGAATGTG+TGG 0.559389 8.4:+33037902 MS.gene97979:CDS
CCGTCTCATGCTATATGCAA+TGG 0.560658 8.4:+33038457 MS.gene97979:CDS
AGAAGGGGAGGTAATAGAAG+GGG 0.561081 8.4:-33036295 None:intergenic
TGTTGATATTTCACACAAGA+GGG 0.562610 8.4:+33037767 MS.gene97979:CDS
TTAGGTCTGCACAAACTGGT+TGG 0.564740 8.4:+33038105 MS.gene97979:CDS
TGGTGATGAGAACATGGAAG+AGG 0.565897 8.4:+33037064 MS.gene97979:CDS
GGAAGATACTCATAATGAAG+AGG 0.568013 8.4:-33037837 None:intergenic
GGGTGCGTCTAGCTCTGGTA+AGG 0.568302 8.4:+33037557 MS.gene97979:CDS
GTGGAAATGGGAGTGAGTTG+GGG 0.568955 8.4:-33036360 None:intergenic
ATTCGTGGGATGAAGTATGA+AGG 0.571493 8.4:+33037534 MS.gene97979:CDS
GAAGTAGCATTAGAGGGTTG+TGG 0.573204 8.4:-33036567 None:intergenic
TGTTGTAGTACTTAGTGTAG+AGG 0.573712 8.4:-33036542 None:intergenic
CTGTTGATATTTCACACAAG+AGG 0.573952 8.4:+33037766 MS.gene97979:CDS
CATTGCATATAGCATGAGAC+GGG 0.574005 8.4:-33038456 None:intergenic
ATAGATAGATGGGCAGAATG+GGG 0.576328 8.4:+33038257 MS.gene97979:CDS
GGTTTGAAGCGGTGCTCCGG+TGG 0.577375 8.4:-33036379 None:intergenic
GTGTTGTTCTCAAAGATGCT+AGG 0.577721 8.4:+33037697 MS.gene97979:CDS
ATTTGTGATCAGAAATTAGA+GGG 0.579266 8.4:+33037459 MS.gene97979:CDS
GGAAGAGGCAGCAACATGGA+TGG 0.581307 8.4:+33037079 MS.gene97979:CDS
TTGCATATGCAGATACAAGC+TGG 0.583868 8.4:+33037279 MS.gene97979:intron
ATGGAAGGCGAAATGTTGAA+TGG 0.584628 8.4:+33037098 MS.gene97979:CDS
CAACTCACTCCCATTTCCAC+CGG 0.584867 8.4:+33036363 MS.gene97979:CDS
GTAATAATCTATACCGGCCA+AGG 0.586629 8.4:+33037408 MS.gene97979:CDS
CCATTGCATATAGCATGAGA+CGG 0.588288 8.4:-33038457 None:intergenic
TGCTGAGTTCTCTCAACGGA+GGG 0.589565 8.4:-33038487 None:intergenic
TTATCGGAAGTAGCATTAGA+GGG 0.591714 8.4:-33036573 None:intergenic
GTATTCAAATATGAGTATGA+AGG 0.592101 8.4:+33036821 MS.gene97979:CDS
TGGCATCCCAGACGAGTGGG+CGG 0.595250 8.4:+33038517 MS.gene97979:CDS
GCAACGTGATGAGGAGAACA+AGG 0.596580 8.4:+33036859 MS.gene97979:CDS
GAGGCAGCAACATGGATGGA+AGG 0.597333 8.4:+33037083 MS.gene97979:CDS
CTCTGATGAAATACAAACCA+CGG 0.598765 8.4:-33037873 None:intergenic
TCGTGGTGACATATGGGGCG+AGG 0.599386 8.4:+33037142 MS.gene97979:CDS
TGGTTGGGGAGTTAGGTCCT+TGG 0.600362 8.4:+33038121 MS.gene97979:CDS
TGAGGAGAAGAGGTTGCATG+AGG 0.600527 8.4:+33036992 MS.gene97979:CDS
GGTAATAGAAGGGGTTTCAA+GGG 0.602104 8.4:-33036286 None:intergenic
TTGTTGTGTTCATATCCAGA+AGG 0.602561 8.4:-33036474 None:intergenic
GGGAGCTGGTGATGAGAACA+TGG 0.604987 8.4:+33037058 MS.gene97979:CDS
GTTGAAGGGCAAACTAAGAT+GGG 0.605405 8.4:+33037672 MS.gene97979:CDS
CCTAAAAGTAGGTCTCTCAA+TGG 0.605488 8.4:+33037315 MS.gene97979:CDS
GCATACTACGCTCTAAAGCA+AGG 0.606571 8.4:-33037488 None:intergenic
GGGACGAGTATGGTTTGAAG+CGG 0.607757 8.4:-33036390 None:intergenic
TAAATCATAACCTTAATATG+GGG 0.609176 8.4:+33036685 MS.gene97979:CDS
TCATATCCAGAAGGGAAATG+AGG 0.611409 8.4:-33036465 None:intergenic
TGTTGTGTTCATATCCAGAA+GGG 0.611765 8.4:-33036473 None:intergenic
ACGTGTGTTAGCAAGTATTG+AGG 0.613466 8.4:+33036899 MS.gene97979:CDS
AGGCAAGTCAGATGCTTGTG+AGG 0.614350 8.4:+33036799 MS.gene97979:CDS
TCGTGGGATGAAGTATGAAG+GGG 0.618537 8.4:+33037536 MS.gene97979:CDS
CATATTCGTGGTGACATATG+GGG 0.619803 8.4:+33037137 MS.gene97979:CDS
TCCAGCTTGAATAAGGTCCA+AGG 0.620502 8.4:-33038138 None:intergenic
TTCTGAAGTTGCGATTATGG+AGG 0.624607 8.4:+33037028 MS.gene97979:CDS
ACATGGAAGAGGCAGCAACA+TGG 0.634502 8.4:+33037075 MS.gene97979:CDS
TCGAGATTAGGGTTATTCGT+GGG 0.635248 8.4:+33037520 MS.gene97979:CDS
CTCAAAAGGGGATCAAGCAT+AGG 0.636242 8.4:+33038072 MS.gene97979:CDS
TGTGATCAGAAATTAGAGGG+TGG 0.640509 8.4:+33037462 MS.gene97979:CDS
TACTGGCTGTGAATGTGTGG+AGG 0.644458 8.4:+33037905 MS.gene97979:CDS
AAATCATAACCTTAATATGG+GGG 0.649898 8.4:+33036686 MS.gene97979:CDS
CTATGGCATCCCAGACGAGT+GGG 0.653271 8.4:+33038514 MS.gene97979:CDS
ACTATGGCATCCCAGACGAG+TGG 0.656197 8.4:+33038513 MS.gene97979:CDS
GGAATCGTGTTGCTCAAAAG+GGG 0.665750 8.4:+33038060 MS.gene97979:CDS
TGGTGTGTTCATATCCAGGA+GGG 0.666587 8.4:-33036509 None:intergenic
ATGCTGAGTTCTCTCAACGG+AGG 0.671604 8.4:-33038488 None:intergenic
TGGTTTGTATTTCATCAGAG+CGG 0.676509 8.4:+33037876 MS.gene97979:CDS
ACACGATTCCGACAATTGGG+AGG 0.682616 8.4:-33038047 None:intergenic
ACTAGTGTGATTGTTTCAGG+CGG 0.687424 8.4:+33037351 MS.gene97979:CDS
CGTGGGATGAAGTATGAAGG+GGG 0.704890 8.4:+33037537 MS.gene97979:CDS
ATAATCTATACCGGCCAAGG+AGG 0.705547 8.4:+33037411 MS.gene97979:CDS
TTGGTGTGTTCATATCCAGG+AGG 0.720395 8.4:-33036510 None:intergenic
ATTGCATATAGCATGAGACG+GGG 0.721884 8.4:-33038455 None:intergenic
TTGCATATAGCATGAGACGG+GGG 0.722787 8.4:-33038454 None:intergenic
TCTATGCTGAGTTCTCTCAA+CGG 0.725900 8.4:-33038491 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGGTTTATAGATAGAT+GGG + chr8.4:33038247-33038266 MS.gene97979:CDS 20.0%
!! AAAATGATAAAGGGATTTAT+CGG - chr8.4:33036592-33036611 None:intergenic 20.0%
!! AAATAAACAAGAATCATGTT+CGG + chr8.4:33036755-33036774 MS.gene97979:CDS 20.0%
!! AAGAAATCATCAAAATGATT+AGG - chr8.4:33036420-33036439 None:intergenic 20.0%
!! ACTAAATCATAACCTTAATA+TGG + chr8.4:33036683-33036702 MS.gene97979:CDS 20.0%
!! CTAAATCATAACCTTAATAT+GGG + chr8.4:33036684-33036703 MS.gene97979:CDS 20.0%
!! TAAATCATAACCTTAATATG+GGG + chr8.4:33036685-33036704 MS.gene97979:CDS 20.0%
!! TAATCTATATCAGGTATTAA+TGG - chr8.4:33038326-33038345 None:intergenic 20.0%
!!! GATTCTTGTTTATTTGAAAT+GGG - chr8.4:33036750-33036769 None:intergenic 20.0%
!!! GATTTTGAGAAAATGATAAA+GGG - chr8.4:33036601-33036620 None:intergenic 20.0%
!!! TGATTCTTGTTTATTTGAAA+TGG - chr8.4:33036751-33036770 None:intergenic 20.0%
! AAATCATAACCTTAATATGG+GGG + chr8.4:33036686-33036705 MS.gene97979:CDS 25.0%
! AATTATTGAGGAGAAGAAAT+TGG + chr8.4:33036932-33036951 MS.gene97979:CDS 25.0%
! AATTTGTGATCAGAAATTAG+AGG + chr8.4:33037458-33037477 MS.gene97979:CDS 25.0%
! ACAAACTGAACAAATACATT+TGG - chr8.4:33038407-33038426 None:intergenic 25.0%
! ACCTGATATAGATTATTCTA+TGG + chr8.4:33038331-33038350 MS.gene97979:CDS 25.0%
! ACTCGTTAATATATCCAAAA+AGG + chr8.4:33038231-33038250 MS.gene97979:CDS 25.0%
! ATAAGTTATTGAGAGTTGAA+GGG + chr8.4:33037658-33037677 MS.gene97979:CDS 25.0%
! ATGTCATTAAATAGCCTAAT+AGG - chr8.4:33037801-33037820 None:intergenic 25.0%
! ATTTGTGATCAGAAATTAGA+GGG + chr8.4:33037459-33037478 MS.gene97979:CDS 25.0%
! CAAAAAGGTTTATAGATAGA+TGG + chr8.4:33038246-33038265 MS.gene97979:CDS 25.0%
! CATCAAAATGATTAGGTTTA+GGG - chr8.4:33036413-33036432 None:intergenic 25.0%
! CTAGGAAGATTAAGAAAATT+GGG + chr8.4:33037715-33037734 MS.gene97979:CDS 25.0%
! GTATTCAAATATGAGTATGA+AGG + chr8.4:33036821-33036840 MS.gene97979:CDS 25.0%
! GTTAATGATAAAGGATCTAT+TGG - chr8.4:33036267-33036286 None:intergenic 25.0%
! TATAAGTTATTGAGAGTTGA+AGG + chr8.4:33037657-33037676 MS.gene97979:CDS 25.0%
! TCATCAAAATGATTAGGTTT+AGG - chr8.4:33036414-33036433 None:intergenic 25.0%
! TCCATAGAATAATCTATATC+AGG - chr8.4:33038335-33038354 None:intergenic 25.0%
!! CATCTATCTATAAACCTTTT+TGG - chr8.4:33038248-33038267 None:intergenic 25.0%
!! TTGATTTTCTTCCTAAAAGT+AGG + chr8.4:33037304-33037323 MS.gene97979:CDS 25.0%
!!! ATACAGGAGTAATTTTAACA+AGG + chr8.4:33038177-33038196 MS.gene97979:CDS 25.0%
!!! ATGTTTATGATGGATTGTAT+AGG + chr8.4:33037583-33037602 MS.gene97979:CDS 25.0%
!!! CGATTTTGAGAAAATGATAA+AGG - chr8.4:33036602-33036621 None:intergenic 25.0%
!!! GCTCAAATTTTAACAATGAA+TGG + chr8.4:33038206-33038225 MS.gene97979:CDS 25.0%
!!! TACAGGAGTAATTTTAACAA+GGG + chr8.4:33038178-33038197 MS.gene97979:CDS 25.0%
ACATCAGATTGTTATGATCA+TGG - chr8.4:33038433-33038452 None:intergenic 30.0%
AGACGTTATAATCATATTCG+TGG + chr8.4:33037125-33037144 MS.gene97979:CDS 30.0%
CAAATATGAGTATGAAGGAT+CGG + chr8.4:33036826-33036845 MS.gene97979:CDS 30.0%
CTGATCACAAATTTGAATTG+AGG - chr8.4:33037451-33037470 None:intergenic 30.0%
GAAGATTAAGAAAATTGGGT+TGG + chr8.4:33037719-33037738 MS.gene97979:CDS 30.0%
GATGATATGGAATTGGATAA+TGG + chr8.4:33037381-33037400 MS.gene97979:CDS 30.0%
GCTAGGAAGATTAAGAAAAT+TGG + chr8.4:33037714-33037733 MS.gene97979:CDS 30.0%
GGATGAGAAAAGAATTATTG+AGG + chr8.4:33036920-33036939 MS.gene97979:CDS 30.0%
GGTAAGGTTTATGTTTATGA+TGG + chr8.4:33037573-33037592 MS.gene97979:CDS 30.0%
GGTGATGTAATAATCTATAC+CGG + chr8.4:33037402-33037421 MS.gene97979:CDS 30.0%
GTTTCAAGGGTTAATGATAA+AGG - chr8.4:33036276-33036295 None:intergenic 30.0%
TAAGGTTATGATTTAGTCCA+AGG - chr8.4:33036680-33036699 None:intergenic 30.0%
TCAACAGAAAGACAATTCAT+AGG - chr8.4:33037753-33037772 None:intergenic 30.0%
TGTTGATATTTCACACAAGA+GGG + chr8.4:33037767-33037786 MS.gene97979:CDS 30.0%
TTATGAGGATGATATGGAAT+TGG + chr8.4:33037374-33037393 MS.gene97979:CDS 30.0%
! AACCACGGAAAAATAGTTTT+TGG - chr8.4:33037861-33037880 None:intergenic 30.0%
! ATGGTGGAATTATGTGTAAT+TGG + chr8.4:33037255-33037274 MS.gene97979:intron 30.0%
! TCAGGTATTAATGGATATGA+TGG - chr8.4:33038317-33038336 None:intergenic 30.0%
! TGATGTTTTCTTGTATATGG+TGG + chr8.4:33037239-33037258 MS.gene97979:intron 30.0%
! TGTATAGGATTATTGATTGC+TGG + chr8.4:33037598-33037617 MS.gene97979:CDS 30.0%
!! AAGCCTTTGATTTTTGAATG+TGG + chr8.4:33038008-33038027 MS.gene97979:CDS 30.0%
!! TGGTGATGTTTTCTTGTATA+TGG + chr8.4:33037236-33037255 MS.gene97979:intron 30.0%
!! TTCCAAAAACTATTTTTCCG+TGG + chr8.4:33037856-33037875 MS.gene97979:CDS 30.0%
AAGCAAACCCTGAAGATTAT+AGG - chr8.4:33037979-33037998 None:intergenic 35.0%
AATTCTTCTTTCTTCCTCCT+TGG - chr8.4:33037428-33037447 None:intergenic 35.0%
ACAATCACACTAGTAGCAAT+TGG - chr8.4:33037345-33037364 None:intergenic 35.0%
AGAGTTTCCCTATAATCTTC+AGG + chr8.4:33037968-33037987 MS.gene97979:CDS 35.0%
AGCAAACCCTGAAGATTATA+GGG - chr8.4:33037978-33037997 None:intergenic 35.0%
AGTGTAGAGGTTAGATTGAT+TGG - chr8.4:33036532-33036551 None:intergenic 35.0%
ATACATTTGGACTCGAACTA+TGG - chr8.4:33038394-33038413 None:intergenic 35.0%
ATCATATTCGTGGTGACATA+TGG + chr8.4:33037135-33037154 MS.gene97979:CDS 35.0%
CTCTGATGAAATACAAACCA+CGG - chr8.4:33037876-33037895 None:intergenic 35.0%
CTGTTGATATTTCACACAAG+AGG + chr8.4:33037766-33037785 MS.gene97979:CDS 35.0%
GAGTTTCCCTATAATCTTCA+GGG + chr8.4:33037969-33037988 MS.gene97979:CDS 35.0%
GGAAGATACTCATAATGAAG+AGG - chr8.4:33037840-33037859 None:intergenic 35.0%
TATAGATAGATGGGCAGAAT+GGG + chr8.4:33038256-33038275 MS.gene97979:CDS 35.0%
TATCGCGATAAAAGGATAGT+TGG + chr8.4:33037192-33037211 MS.gene97979:intron 35.0%
TATGGTTGTATCGCGATAAA+AGG + chr8.4:33037184-33037203 MS.gene97979:intron 35.0%
TCATATTCGTGGTGACATAT+GGG + chr8.4:33037136-33037155 MS.gene97979:CDS 35.0%
TGTTGTAGTACTTAGTGTAG+AGG - chr8.4:33036545-33036564 None:intergenic 35.0%
TGTTGTGTTCATATCCAGAA+GGG - chr8.4:33036476-33036495 None:intergenic 35.0%
TTAGAGCGTAGTATGCATTA+CGG + chr8.4:33037495-33037514 MS.gene97979:CDS 35.0%
TTATAGATAGATGGGCAGAA+TGG + chr8.4:33038255-33038274 MS.gene97979:CDS 35.0%
TTATCGGAAGTAGCATTAGA+GGG - chr8.4:33036576-33036595 None:intergenic 35.0%
TTGTTGTGTTCATATCCAGA+AGG - chr8.4:33036477-33036496 None:intergenic 35.0%
TTTATCGGAAGTAGCATTAG+AGG - chr8.4:33036577-33036596 None:intergenic 35.0%
! TCAGTCAATATTTCTGACCT+TGG + chr8.4:33036660-33036679 MS.gene97979:CDS 35.0%
! TGGTTTGTATTTCATCAGAG+CGG + chr8.4:33037876-33037895 MS.gene97979:CDS 35.0%
!! AGTAGCTCTGGTTAAGAAAA+AGG + chr8.4:33036779-33036798 MS.gene97979:CDS 35.0%
!! ATTGATTGCTGGTTTGAAGA+AGG + chr8.4:33037609-33037628 MS.gene97979:CDS 35.0%
!! TGTTTCTGAAGTTGCGATTA+TGG + chr8.4:33037025-33037044 MS.gene97979:CDS 35.0%
!! TTGATTGCTGGTTTGAAGAA+GGG + chr8.4:33037610-33037629 MS.gene97979:CDS 35.0%
AAGGGAAATGAGGTTGTTGT+TGG - chr8.4:33036458-33036477 None:intergenic 40.0%
AATCAATCTCATTTCCCTCC+TGG + chr8.4:33036495-33036514 MS.gene97979:CDS 40.0%
ACATCACCAACATAAACTCC+AGG - chr8.4:33037225-33037244 None:intergenic 40.0%
ACTAGTGTGATTGTTTCAGG+CGG + chr8.4:33037351-33037370 MS.gene97979:CDS 40.0%
AGAGAACTCAGCATAGACTA+TGG + chr8.4:33038497-33038516 MS.gene97979:CDS 40.0%
AGAGGGAGAATGTTCCTATT+AGG + chr8.4:33037784-33037803 MS.gene97979:CDS 40.0%
AGGTAATAGAAGGGGTTTCA+AGG - chr8.4:33036290-33036309 None:intergenic 40.0%
AGTTGAAGGGCAAACTAAGA+TGG + chr8.4:33037671-33037690 MS.gene97979:CDS 40.0%
ATAGATAGATGGGCAGAATG+GGG + chr8.4:33038257-33038276 MS.gene97979:CDS 40.0%
ATCGAGATTAGGGTTATTCG+TGG + chr8.4:33037519-33037538 MS.gene97979:CDS 40.0%
ATGGAAGGCGAAATGTTGAA+TGG + chr8.4:33037098-33037117 MS.gene97979:CDS 40.0%
ATTAGGTTTAGGGACGAGTA+TGG - chr8.4:33036403-33036422 None:intergenic 40.0%
ATTCGTGGGATGAAGTATGA+AGG + chr8.4:33037534-33037553 MS.gene97979:CDS 40.0%
ATTGCATATAGCATGAGACG+GGG - chr8.4:33038458-33038477 None:intergenic 40.0%
CAAAATCCACCCCCATATTA+AGG - chr8.4:33036698-33036717 None:intergenic 40.0%
CATATTCGTGGTGACATATG+GGG + chr8.4:33037137-33037156 MS.gene97979:CDS 40.0%
CATTGCATATAGCATGAGAC+GGG - chr8.4:33038459-33038478 None:intergenic 40.0%
CCATTGCATATAGCATGAGA+CGG - chr8.4:33038460-33038479 None:intergenic 40.0%
CCTAAAAGTAGGTCTCTCAA+TGG + chr8.4:33037315-33037334 MS.gene97979:CDS 40.0%
GATTGAGGGTTGACATGTTA+GGG - chr8.4:33036341-33036360 None:intergenic 40.0%
GCTACTAGTGTGATTGTTTC+AGG + chr8.4:33037348-33037367 MS.gene97979:CDS 40.0%
GGGCCACATTCAAAAATCAA+AGG - chr8.4:33038014-33038033 None:intergenic 40.0%
GGTAATAGAAGGGGTTTCAA+GGG - chr8.4:33036289-33036308 None:intergenic 40.0%
GGTGAGAAGAAGTTTGAGAA+GGG - chr8.4:33036314-33036333 None:intergenic 40.0%
GGTTTGTATTTCATCAGAGC+GGG + chr8.4:33037877-33037896 MS.gene97979:CDS 40.0%
GTAATAATCTATACCGGCCA+AGG + chr8.4:33037408-33037427 MS.gene97979:CDS 40.0%
GTGAGAAGAAGTTTGAGAAG+GGG - chr8.4:33036313-33036332 None:intergenic 40.0%
GTTGAAGGGCAAACTAAGAT+GGG + chr8.4:33037672-33037691 MS.gene97979:CDS 40.0%
TAAAGGCTCCAGCTTGAATA+AGG - chr8.4:33038148-33038167 None:intergenic 40.0%
TAATGAAGGACCGAGAGTTA+TGG + chr8.4:33037166-33037185 MS.gene97979:intron 40.0%
TAGAGCGTAGTATGCATTAC+GGG + chr8.4:33037496-33037515 MS.gene97979:CDS 40.0%
TCATAACCTTAATATGGGGG+TGG + chr8.4:33036689-33036708 MS.gene97979:CDS 40.0%
TCATATCCAGAAGGGAAATG+AGG - chr8.4:33036468-33036487 None:intergenic 40.0%
TCCTGTATACTCGCAGATAA+AGG - chr8.4:33038165-33038184 None:intergenic 40.0%
TCGAGATTAGGGTTATTCGT+GGG + chr8.4:33037520-33037539 MS.gene97979:CDS 40.0%
TCGATACCTGGAGTTTATGT+TGG + chr8.4:33037216-33037235 MS.gene97979:intron 40.0%
TCTATGCTGAGTTCTCTCAA+CGG - chr8.4:33038494-33038513 None:intergenic 40.0%
TGATTGGTGTGTTCATATCC+AGG - chr8.4:33036516-33036535 None:intergenic 40.0%
TGGTGAGAAGAAGTTTGAGA+AGG - chr8.4:33036315-33036334 None:intergenic 40.0%
TGGTTTGAAGAAGGGATGAA+TGG + chr8.4:33037618-33037637 MS.gene97979:CDS 40.0%
TGTGATCAGAAATTAGAGGG+TGG + chr8.4:33037462-33037481 MS.gene97979:CDS 40.0%
TTCGTGGGATGAAGTATGAA+GGG + chr8.4:33037535-33037554 MS.gene97979:CDS 40.0%
TTGCATATGCAGATACAAGC+TGG + chr8.4:33037279-33037298 MS.gene97979:intron 40.0%
! ACGTGTGTTAGCAAGTATTG+AGG + chr8.4:33036899-33036918 MS.gene97979:CDS 40.0%
! AGGGAAATGAGGTTGTTGTT+GGG - chr8.4:33036457-33036476 None:intergenic 40.0%
! ATTGGCTGACGAAGTTTTTG+TGG + chr8.4:33036950-33036969 MS.gene97979:CDS 40.0%
! GTGTTGTTCTCAAAGATGCT+AGG + chr8.4:33037697-33037716 MS.gene97979:CDS 40.0%
! TGCATTTTTGCAACGTGATG+AGG + chr8.4:33036850-33036869 MS.gene97979:CDS 40.0%
!! AGCATAGGTTGGAAGTGTTT+AGG + chr8.4:33038087-33038106 MS.gene97979:CDS 40.0%
!! CCATTGAGAGACCTACTTTT+AGG - chr8.4:33037318-33037337 None:intergenic 40.0%
!! TAATATGGGGGTGGATTTTG+TGG + chr8.4:33036698-33036717 MS.gene97979:CDS 40.0%
!! TTCTGAAGTTGCGATTATGG+AGG + chr8.4:33037028-33037047 MS.gene97979:CDS 40.0%
AAACTCTCCTCCATTCTTCC+TGG - chr8.4:33037955-33037974 None:intergenic 45.0%
AAAGGGGATCAAGCATAGGT+TGG + chr8.4:33038076-33038095 MS.gene97979:CDS 45.0%
AGAAGAAGTTTGAGAAGGGG+AGG - chr8.4:33036310-33036329 None:intergenic 45.0%
AGAAGGGGAGGTAATAGAAG+GGG - chr8.4:33036298-33036317 None:intergenic 45.0%
AGCAACACGATTCCGACAAT+TGG - chr8.4:33038054-33038073 None:intergenic 45.0%
AGGCGGTTATGAGGATGATA+TGG + chr8.4:33037368-33037387 MS.gene97979:CDS 45.0%
ATAATCTATACCGGCCAAGG+AGG + chr8.4:33037411-33037430 MS.gene97979:CDS 45.0%
ATATGGGGCGAGGTTAATGA+AGG + chr8.4:33037152-33037171 MS.gene97979:intron 45.0%
CAACAACCTCATTTCCCTTC+TGG + chr8.4:33036459-33036478 MS.gene97979:CDS 45.0%
CAATGCTTCTGCAGCTTCAA+TGG + chr8.4:33036632-33036651 MS.gene97979:CDS 45.0%
CCGTCTCATGCTATATGCAA+TGG + chr8.4:33038457-33038476 MS.gene97979:CDS 45.0%
CGCGATACAACCATAACTCT+CGG - chr8.4:33037179-33037198 None:intergenic 45.0%
CTCAAAAGGGGATCAAGCAT+AGG + chr8.4:33038072-33038091 MS.gene97979:CDS 45.0%
GAAGTAGCATTAGAGGGTTG+TGG - chr8.4:33036570-33036589 None:intergenic 45.0%
GAGAAGGGGAGGTAATAGAA+GGG - chr8.4:33036299-33036318 None:intergenic 45.0%
GATGATTTCTTCACCACCCA+AGG + chr8.4:33036429-33036448 MS.gene97979:CDS 45.0%
GATTGTTTCAGGCGGTTATG+AGG + chr8.4:33037359-33037378 MS.gene97979:CDS 45.0%
GCAACACGATTCCGACAATT+GGG - chr8.4:33038053-33038072 None:intergenic 45.0%
GCATTACGGGATCGAGATTA+GGG + chr8.4:33037509-33037528 MS.gene97979:CDS 45.0%
GCCTTTATCTGCGAGTATAC+AGG + chr8.4:33038161-33038180 MS.gene97979:CDS 45.0%
GGATTGAGGGTTGACATGTT+AGG - chr8.4:33036342-33036361 None:intergenic 45.0%
GGGTTGACATGTTAGGGATT+TGG - chr8.4:33036335-33036354 None:intergenic 45.0%
GTGTTTAGGTCTGCACAAAC+TGG + chr8.4:33038101-33038120 MS.gene97979:CDS 45.0%
TAGATAGATGGGCAGAATGG+GGG + chr8.4:33038258-33038277 MS.gene97979:CDS 45.0%
TAGGTCTGCACAAACTGGTT+GGG + chr8.4:33038106-33038125 MS.gene97979:CDS 45.0%
TCCAGCTTGAATAAGGTCCA+AGG - chr8.4:33038141-33038160 None:intergenic 45.0%
TCCTTGGACCTTATTCAAGC+TGG + chr8.4:33038137-33038156 MS.gene97979:CDS 45.0%
TCGTGGGATGAAGTATGAAG+GGG + chr8.4:33037536-33037555 MS.gene97979:CDS 45.0%
TGAAGTTGCGATTATGGAGG+CGG + chr8.4:33037031-33037050 MS.gene97979:CDS 45.0%
TGAGAAGGGGAGGTAATAGA+AGG - chr8.4:33036300-33036319 None:intergenic 45.0%
TGCATTACGGGATCGAGATT+AGG + chr8.4:33037508-33037527 MS.gene97979:CDS 45.0%
TGGGAGGACAAGAGCAAAAA+GGG - chr8.4:33038034-33038053 None:intergenic 45.0%
TGGTGTGTTCATATCCAGGA+GGG - chr8.4:33036512-33036531 None:intergenic 45.0%
TGTGGAGGCAGATAAGAGAT+TGG + chr8.4:33036968-33036987 MS.gene97979:CDS 45.0%
TTAGGTCTGCACAAACTGGT+TGG + chr8.4:33038105-33038124 MS.gene97979:CDS 45.0%
TTGCATATAGCATGAGACGG+GGG - chr8.4:33038457-33038476 None:intergenic 45.0%
TTGGGAGGACAAGAGCAAAA+AGG - chr8.4:33038035-33038054 None:intergenic 45.0%
TTGGTGTGTTCATATCCAGG+AGG - chr8.4:33036513-33036532 None:intergenic 45.0%
! AGAGATTGGTTGAGGAGAAG+AGG + chr8.4:33036982-33037001 MS.gene97979:CDS 45.0%
! AGGTTGTTGTTGGGAACCTT+GGG - chr8.4:33036448-33036467 None:intergenic 45.0%
! CGGAATCGTGTTGCTCAAAA+GGG + chr8.4:33038059-33038078 MS.gene97979:CDS 45.0%
! CTTCAGGGTTTGCTTGTGAA+AGG + chr8.4:33037984-33038003 MS.gene97979:CDS 45.0%
! GGAATCGTGTTGCTCAAAAG+GGG + chr8.4:33038060-33038079 MS.gene97979:CDS 45.0%
! GGCAGATAAGAGATTGGTTG+AGG + chr8.4:33036974-33036993 MS.gene97979:CDS 45.0%
! TCGGAATCGTGTTGCTCAAA+AGG + chr8.4:33038058-33038077 MS.gene97979:CDS 45.0%
! TGCTTTTGCTCCAGGAAGAA+TGG + chr8.4:33037942-33037961 MS.gene97979:CDS 45.0%
! TGGTGATGAGAACATGGAAG+AGG + chr8.4:33037064-33037083 MS.gene97979:CDS 45.0%
! TTTTGCTCCAGGAAGAATGG+AGG + chr8.4:33037945-33037964 MS.gene97979:CDS 45.0%
!! AGGATAGTTGGTTCGATACC+TGG + chr8.4:33037204-33037223 MS.gene97979:intron 45.0%
!! GCATACTACGCTCTAAAGCA+AGG - chr8.4:33037491-33037510 None:intergenic 45.0%
!! TGAAGCTGCAGAAGCATTGT+TGG - chr8.4:33036631-33036650 None:intergenic 45.0%
ACACGATTCCGACAATTGGG+AGG - chr8.4:33038050-33038069 None:intergenic 50.0%
ACATGGAAGAGGCAGCAACA+TGG + chr8.4:33037075-33037094 MS.gene97979:CDS 50.0%
AGATAGATGGGCAGAATGGG+GGG + chr8.4:33038259-33038278 MS.gene97979:CDS 50.0%
AGGCAAGTCAGATGCTTGTG+AGG + chr8.4:33036799-33036818 MS.gene97979:CDS 50.0%
AGGTCTGCACAAACTGGTTG+GGG + chr8.4:33038107-33038126 MS.gene97979:CDS 50.0%
ATCATGTTCGGCAGTAGCTC+TGG + chr8.4:33036767-33036786 MS.gene97979:CDS 50.0%
ATGCTGAGTTCTCTCAACGG+AGG - chr8.4:33038491-33038510 None:intergenic 50.0%
CAACTCACTCCCATTTCCAC+CGG + chr8.4:33036363-33036382 MS.gene97979:CDS 50.0%
CACAAACTGGTTGGGGAGTT+AGG + chr8.4:33038114-33038133 MS.gene97979:CDS 50.0%
CGTGGGATGAAGTATGAAGG+GGG + chr8.4:33037537-33037556 MS.gene97979:CDS 50.0%
CTTCTTTCTTCCTCCTTGGC+CGG - chr8.4:33037424-33037443 None:intergenic 50.0%
GCAACGTGATGAGGAGAACA+AGG + chr8.4:33036859-33036878 MS.gene97979:CDS 50.0%
GCTCTTGTCCTCCCAATTGT+CGG + chr8.4:33038039-33038058 MS.gene97979:CDS 50.0%
GGGACGAGTATGGTTTGAAG+CGG - chr8.4:33036393-33036412 None:intergenic 50.0%
GGTGGAAATGGGAGTGAGTT+GGG - chr8.4:33036364-33036383 None:intergenic 50.0%
GTGGAAATGGGAGTGAGTTG+GGG - chr8.4:33036363-33036382 None:intergenic 50.0%
TGAGGAGAAGAGGTTGCATG+AGG + chr8.4:33036992-33037011 MS.gene97979:CDS 50.0%
TGCTGAGTTCTCTCAACGGA+GGG - chr8.4:33038490-33038509 None:intergenic 50.0%
TGTGTGGAGGCTTGTACTGA+TGG + chr8.4:33037918-33037937 MS.gene97979:CDS 50.0%
! AGCTACTGGCTGTGAATGTG+TGG + chr8.4:33037902-33037921 MS.gene97979:CDS 50.0%
! GAGGTTGTTGTTGGGAACCT+TGG - chr8.4:33036449-33036468 None:intergenic 50.0%
! GGCTGACGAAGTTTTTGTGG+AGG + chr8.4:33036953-33036972 MS.gene97979:CDS 50.0%
! TACTGGCTGTGAATGTGTGG+AGG + chr8.4:33037905-33037924 MS.gene97979:CDS 50.0%
! TATGGTTTGAAGCGGTGCTC+CGG - chr8.4:33036385-33036404 None:intergenic 50.0%
! TTGTTGTTGGGAACCTTGGG+TGG - chr8.4:33036445-33036464 None:intergenic 50.0%
ACTATGGCATCCCAGACGAG+TGG + chr8.4:33038513-33038532 MS.gene97979:CDS 55.0%
CATCAGAGCGGGAAAGCTAC+TGG + chr8.4:33037888-33037907 MS.gene97979:CDS 55.0%
CGGTGGAAATGGGAGTGAGT+TGG - chr8.4:33036365-33036384 None:intergenic 55.0%
CTATGGCATCCCAGACGAGT+GGG + chr8.4:33038514-33038533 MS.gene97979:CDS 55.0%
GAGGCAGCAACATGGATGGA+AGG + chr8.4:33037083-33037102 MS.gene97979:CDS 55.0%
GCGATTATGGAGGCGGAGAT+GGG + chr8.4:33037038-33037057 MS.gene97979:CDS 55.0%
GGAAGAGGCAGCAACATGGA+TGG + chr8.4:33037079-33037098 MS.gene97979:CDS 55.0%
TGCGATTATGGAGGCGGAGA+TGG + chr8.4:33037037-33037056 MS.gene97979:CDS 55.0%
TGGTTGGGGAGTTAGGTCCT+TGG + chr8.4:33038121-33038140 MS.gene97979:CDS 55.0%
! GGGAGCTGGTGATGAGAACA+TGG + chr8.4:33037058-33037077 MS.gene97979:CDS 55.0%
! GGGAGTGAGTTGGGGATTGA+GGG - chr8.4:33036355-33036374 None:intergenic 55.0%
! TGGGAGTGAGTTGGGGATTG+AGG - chr8.4:33036356-33036375 None:intergenic 55.0%
!!! CTGATGGCTGCTTTTGCTCC+AGG + chr8.4:33037934-33037953 MS.gene97979:CDS 55.0%
AAGCGGTGCTCCGGTGGAAA+TGG - chr8.4:33036376-33036395 None:intergenic 60.0%
AGCGGTGCTCCGGTGGAAAT+GGG - chr8.4:33036375-33036394 None:intergenic 60.0%
TCGTGGTGACATATGGGGCG+AGG + chr8.4:33037142-33037161 MS.gene97979:CDS 60.0%
!! GGGTGCGTCTAGCTCTGGTA+AGG + chr8.4:33037557-33037576 MS.gene97979:CDS 60.0%
ATGGAGGCGGAGATGGGAGC+TGG + chr8.4:33037044-33037063 MS.gene97979:CDS 65.0%
TGGCATCCCAGACGAGTGGG+CGG + chr8.4:33038517-33038536 MS.gene97979:CDS 65.0%
! GAAGGGGGTGCGTCTAGCTC+TGG + chr8.4:33037552-33037571 MS.gene97979:CDS 65.0%
! GGTTTGAAGCGGTGCTCCGG+TGG - chr8.4:33036382-33036401 None:intergenic 65.0%
AGCTTGCCCGCCCACTCGTC+TGG - chr8.4:33038527-33038546 None:intergenic 70.0%
GCTTGCCCGCCCACTCGTCT+GGG - chr8.4:33038526-33038545 None:intergenic 70.0%
GGCATCCCAGACGAGTGGGC+GGG + chr8.4:33038518-33038537 MS.gene97979:CDS 70.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 33036258 33038562 33036258 ID=MS.gene97979
chr8.4 mRNA 33036258 33038562 33036258 ID=MS.gene97979.t1;Parent=MS.gene97979
chr8.4 exon 33036258 33037163 33036258 ID=MS.gene97979.t1.exon1;Parent=MS.gene97979.t1
chr8.4 CDS 33036258 33037163 33036258 ID=cds.MS.gene97979.t1;Parent=MS.gene97979.t1
chr8.4 exon 33037291 33038562 33037291 ID=MS.gene97979.t1.exon2;Parent=MS.gene97979.t1
chr8.4 CDS 33037291 33038562 33037291 ID=cds.MS.gene97979.t1;Parent=MS.gene97979.t1
Gene Sequence

>MS.gene97979

ATGAATCCAATAGATCCTTTATCATTAACCCTTGAAACCCCTTCTATTACCTCCCCTTCTCAAACTTCTTCTCACCAAATCCCTAACATGTCAACCCTCAATCCCCAACTCACTCCCATTTCCACCGGAGCACCGCTTCAAACCATACTCGTCCCTAAACCTAATCATTTTGATGATTTCTTCACCACCCAAGGTTCCCAACAACAACCTCATTTCCCTTCTGGATATGAACACAACAATCAATCTCATTTCCCTCCTGGATATGAACACACCAATCAATCTAACCTCTACACTAAGTACTACAACATTCCACAACCCTCTAATGCTACTTCCGATAAATCCCTTTATCATTTTCTCAAAATCGATGATTCCAACAATGCTTCTGCAGCTTCAATGGAGAACTCAGTCAATATTTCTGACCTTGGACTAAATCATAACCTTAATATGGGGGTGGATTTTGTGGATGATAGAAATGATCGATCTATTGTGCCCATTTCAAATAAACAAGAATCATGTTCGGCAGTAGCTCTGGTTAAGAAAAAGGCAAGTCAGATGCTTGTGAGGTATTCAAATATGAGTATGAAGGATCGGATGCATTTTTGCAACGTGATGAGGAGAACAAGGATGACTTTTGATTCGCTACGTGTGTTAGCAAGTATTGAGGATGAGAAAAGAATTATTGAGGAGAAGAAATTGGCTGACGAAGTTTTTGTGGAGGCAGATAAGAGATTGGTTGAGGAGAAGAGGTTGCATGAGGATATGATATTTGTTTCTGAAGTTGCGATTATGGAGGCGGAGATGGGAGCTGGTGATGAGAACATGGAAGAGGCAGCAACATGGATGGAAGGCGAAATGTTGAATGGAAAAAGACGTTATAATCATATTCGTGGTGACATATGGGGCGAGGTTAATGAAGGACCGAGAGTTATGGTTGTATCGCGATAAAAGGATAGTTGGTTCGATACCTGGAGTTTATGTTGGTGATGTTTTCTTGTATATGGTGGAATTATGTGTAATTGGTTTGCATATGCAGATACAAGCTGGCATTGATTTTCTTCCTAAAAGTAGGTCTCTCAATGGCGAGCCAATTGCTACTAGTGTGATTGTTTCAGGCGGTTATGAGGATGATATGGAATTGGATAATGGTGATGTAATAATCTATACCGGCCAAGGAGGAAGAAAGAAGAATTCCTCAATTCAAATTTGTGATCAGAAATTAGAGGGTGGAAACCTTGCTTTAGAGCGTAGTATGCATTACGGGATCGAGATTAGGGTTATTCGTGGGATGAAGTATGAAGGGGGTGCGTCTAGCTCTGGTAAGGTTTATGTTTATGATGGATTGTATAGGATTATTGATTGCTGGTTTGAAGAAGGGATGAATGGTTTTAAAGTTTTTAAGTATAAGTTATTGAGAGTTGAAGGGCAAACTAAGATGGGTAGTGTTGTTCTCAAAGATGCTAGGAAGATTAAGAAAATTGGGTTGGATGTTGAGCCTATGAATTGTCTTTCTGTTGATATTTCACACAAGAGGGAGAATGTTCCTATTAGGCTATTTAATGACATAGATGACAATGAAGAACCTCTTCATTATGAGTATCTTCCAAAAACTATTTTTCCGTGGTTTGTATTTCATCAGAGCGGGAAAGCTACTGGCTGTGAATGTGTGGAGGCTTGTACTGATGGCTGCTTTTGCTCCAGGAAGAATGGAGGAGAGTTTCCCTATAATCTTCAGGGTTTGCTTGTGAAAGGTAAGCCTTTGATTTTTGAATGTGGCCCTTTTTGCTCTTGTCCTCCCAATTGTCGGAATCGTGTTGCTCAAAAGGGGATCAAGCATAGGTTGGAAGTGTTTAGGTCTGCACAAACTGGTTGGGGAGTTAGGTCCTTGGACCTTATTCAAGCTGGAGCCTTTATCTGCGAGTATACAGGAGTAATTTTAACAAGGGAACAAGCTCAAATTTTAACAATGAATGGCGACTCGTTAATATATCCAAAAAGGTTTATAGATAGATGGGCAGAATGGGGGGATTTGTCTCAGATATACAATAATTATGTTCGTCCATCATATCCATTAATACCTGATATAGATTATTCTATGGATATATCAACACTGCGAAATGTTGCTAGCTATTTGAGCCATAGTTCGAGTCCAAATGTATTTGTTCAGTTTGTTCTCCATGATCATAACAATCTGATGTTCCCCCGTCTCATGCTATATGCAATGGAGAACATCCCTCCGTTGAGAGAACTCAGCATAGACTATGGCATCCCAGACGAGTGGGCGGGCAAGCTACCAGTATGTATGTAA
Protein sequence

>MS.gene97979.t1

MNPIDPLSLTLETPSITSPSQTSSHQIPNMSTLNPQLTPISTGAPLQTILVPKPNHFDDFFTTQGSQQQPHFPSGYEHNNQSHFPPGYEHTNQSNLYTKYYNIPQPSNATSDKSLYHFLKIDDSNNASAASMENSVNISDLGLNHNLNMGVDFVDDRNDRSIVPISNKQESCSAVALVKKKASQMLVRYSNMSMKDRMHFCNVMRRTRMTFDSLRVLASIEDEKRIIEEKKLADEVFVEADKRLVEEKRLHEDMIFVSEVAIMEAEMGAGDENMEEAATWMEGEMLNGKRRYNHIRGDIWGEIQAGIDFLPKSRSLNGEPIATSVIVSGGYEDDMELDNGDVIIYTGQGGRKKNSSIQICDQKLEGGNLALERSMHYGIEIRVIRGMKYEGGASSSGKVYVYDGLYRIIDCWFEEGMNGFKVFKYKLLRVEGQTKMGSVVLKDARKIKKIGLDVEPMNCLSVDISHKRENVPIRLFNDIDDNEEPLHYEYLPKTIFPWFVFHQSGKATGCECVEACTDGCFCSRKNGGEFPYNLQGLLVKGKPLIFECGPFCSCPPNCRNRVAQKGIKHRLEVFRSAQTGWGVRSLDLIQAGAFICEYTGVILTREQAQILTMNGDSLIYPKRFIDRWAEWGDLSQIYNNYVRPSYPLIPDIDYSMDISTLRNVASYLSHSSSPNVFVQFVLHDHNNLMFPRLMLYAMENIPPLRELSIDYGIPDEWAGKLPVCM