AlfalfaGEDB | MS.gene98098

M. sativa cultivar XinJiangDaYe / MS.gene98098

KEGG: -
GO: -
PFAM: PF03195.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98098.t1	PNX82010.1	80.9	157	30	0	1	157	1	157	2.60E-68	268.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98098.t1	P59467	68.5	92	29	0	4	95	4	95	1.5e-32	140.6

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98098.t1	A0A2K3LTZ7	80.9	157	30	0	1	157	1	157	1.9e-68	268.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene98098.t1	TF	LOB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98098.t1	MTR_5g093010	76.433	157	25	1	1	157	1	145	6.15e-86	248
MS.gene98098.t1	MTR_7g028905	95.690	116	5	0	1	116	1	116	2.40e-82	238
MS.gene98098.t1	MTR_2g068760	50.476	105	52	0	6	110	14	118	1.16e-38	129
MS.gene98098.t1	MTR_5g083230	51.376	109	53	0	6	114	7	115	6.91e-38	127
MS.gene98098.t1	MTR_5g083960	46.970	132	70	0	6	137	40	171	1.12e-37	127
MS.gene98098.t1	MTR_4g083680	50.943	106	52	0	6	111	16	121	8.64e-37	125
MS.gene98098.t1	MTR_3g031660	48.148	108	56	0	6	113	8	115	2.88e-36	123
MS.gene98098.t1	MTR_3g094690	52.830	106	50	0	6	111	48	153	3.06e-36	125
MS.gene98098.t1	MTR_4g105170	51.402	107	52	0	6	112	12	118	1.25e-35	122
MS.gene98098.t1	MTR_3g071590	53.000	100	47	0	6	105	9	108	1.42e-35	121
MS.gene98098.t1	MTR_7g033800	46.774	124	57	1	6	120	12	135	5.39e-35	120
MS.gene98098.t1	MTR_3g077240	50.926	108	50	1	6	110	11	118	1.25e-34	120
MS.gene98098.t1	MTR_4g060950	46.429	112	59	1	1	111	20	131	2.79e-34	119
MS.gene98098.t1	MTR_3g094690	52.830	106	49	1	6	111	48	152	4.15e-34	119
MS.gene98098.t1	MTR_3g452660	49.550	111	56	0	6	116	8	118	5.17e-34	121
MS.gene98098.t1	MTR_3g073690	47.573	103	54	0	6	108	10	112	8.27e-34	118
MS.gene98098.t1	MTR_2g093310	44.144	111	62	0	1	111	29	139	1.15e-33	117
MS.gene98098.t1	MTR_6g005070	43.077	130	74	0	6	135	10	139	7.36e-33	114
MS.gene98098.t1	MTR_5g075020	45.865	133	68	1	6	138	44	172	1.84e-32	115
MS.gene98098.t1	MTR_5g017950	48.544	103	53	0	6	108	9	111	2.32e-32	117
MS.gene98098.t1	MTR_5g075020	45.865	133	68	1	6	138	48	176	2.35e-32	115
MS.gene98098.t1	MTR_1g095850	40.845	142	76	1	6	147	73	206	2.64e-32	115
MS.gene98098.t1	MTR_8g040900	50.467	107	51	1	6	112	8	112	4.51e-32	114
MS.gene98098.t1	MTR_6g005080	48.000	100	52	0	6	105	11	110	9.78e-31	108
MS.gene98098.t1	MTR_3g071420	46.392	97	52	0	6	102	15	111	5.39e-30	107
MS.gene98098.t1	MTR_5g083010	44.554	101	56	0	6	106	12	112	9.62e-30	107
MS.gene98098.t1	MTR_7g096530	46.154	104	55	1	6	108	15	118	9.78e-30	108
MS.gene98098.t1	MTR_6g011250	36.111	144	91	1	4	146	8	151	1.77e-28	103
MS.gene98098.t1	MTR_1g070220	48.000	100	51	1	6	104	16	115	4.31e-28	103
MS.gene98098.t1	MTR_6g027710	43.396	106	59	1	6	110	41	146	7.01e-28	103
MS.gene98098.t1	MTR_8g079660	40.952	105	62	0	2	106	5	109	1.14e-26	101
MS.gene98098.t1	MTR_7g096610	39.007	141	83	2	6	143	9	149	5.03e-26	98.6
MS.gene98098.t1	MTR_6g011230	41.414	99	58	0	4	102	10	108	6.89e-26	97.8
MS.gene98098.t1	MTR_8g036085	41.509	106	61	1	6	110	24	129	1.26e-25	97.8
MS.gene98098.t1	MTR_1g070205	44.118	102	56	1	6	106	13	114	2.24e-25	97.4
MS.gene98098.t1	MTR_7g074990	44.554	101	55	1	6	105	21	121	2.95e-25	97.1
MS.gene98098.t1	MTR_8g079580	43.810	105	58	1	2	105	5	109	2.19e-24	94.7
MS.gene98098.t1	MTR_6g011200	37.273	110	68	1	4	112	8	117	3.02e-24	94.7
MS.gene98098.t1	MTR_5g080470	41.176	102	59	1	6	106	8	109	2.18e-23	91.3
MS.gene98098.t1	MTR_8g079620	37.143	105	66	0	2	106	5	109	4.45e-23	91.3
MS.gene98098.t1	MTR_6g027700	37.405	131	69	3	6	123	8	138	4.71e-23	90.5
MS.gene98098.t1	MTR_8g036105	41.176	102	59	1	6	106	19	120	5.58e-23	90.5
MS.gene98098.t1	MTR_4g099230	37.037	108	67	1	6	112	3	110	1.23e-22	88.2
MS.gene98098.t1	MTR_8g017090	39.604	101	61	0	6	106	13	113	6.99e-22	89.4
MS.gene98098.t1	MTR_4g107450	42.000	100	58	0	6	105	11	110	1.12e-21	89.0
MS.gene98098.t1	MTR_5g085390	36.283	113	70	2	4	114	8	120	7.38e-21	85.5
MS.gene98098.t1	MTR_7g075230	39.423	104	60	2	6	106	13	116	7.39e-21	84.7
MS.gene98098.t1	MTR_2g100020	34.653	101	66	0	6	106	20	120	4.17e-19	81.6
MS.gene98098.t1	MTR_4g088035	33.721	86	57	0	6	91	7	92	7.53e-14	67.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98098.t1	AT3G26660	66.990	103	34	0	4	106	4	106	1.40e-48	153
MS.gene98098.t1	AT3G26620	66.990	103	34	0	4	106	4	106	1.78e-48	152
MS.gene98098.t1	AT2G30130	52.778	108	51	0	6	113	9	116	8.15e-39	130
MS.gene98098.t1	AT1G31320	53.097	113	52	1	6	117	14	126	2.42e-36	124
MS.gene98098.t1	AT2G30340	50.943	106	52	0	6	111	53	158	3.22e-35	123
MS.gene98098.t1	AT2G30340	50.943	106	52	0	6	111	54	159	3.48e-35	123
MS.gene98098.t1	AT1G07900	43.902	123	64	1	6	128	34	151	5.05e-35	120
MS.gene98098.t1	AT5G63090	46.957	115	61	0	6	120	12	126	1.07e-34	120
MS.gene98098.t1	AT5G63090	46.957	115	61	0	6	120	12	126	1.07e-34	120
MS.gene98098.t1	AT5G63090	46.957	115	61	0	6	120	12	126	1.07e-34	120
MS.gene98098.t1	AT5G63090	46.957	115	61	0	6	120	12	126	1.07e-34	120
MS.gene98098.t1	AT2G28500	45.946	111	60	0	1	111	51	161	2.45e-34	120
MS.gene98098.t1	AT3G27650	50.000	100	50	0	6	105	40	139	1.50e-33	116
MS.gene98098.t1	AT5G66870	48.515	101	52	0	6	106	8	108	8.61e-33	118
MS.gene98098.t1	AT2G40470	52.885	104	49	0	6	109	21	124	1.21e-32	115
MS.gene98098.t1	AT2G40470	52.885	104	49	0	6	109	46	149	1.97e-32	115
MS.gene98098.t1	AT1G16530	50.485	103	50	1	6	107	15	117	4.27e-32	112
MS.gene98098.t1	AT1G65620	48.077	104	54	0	6	109	10	113	5.59e-32	113
MS.gene98098.t1	AT1G65620	48.077	104	54	0	6	109	10	113	5.59e-32	113
MS.gene98098.t1	AT1G65620	48.077	104	54	0	6	109	10	113	5.59e-32	113
MS.gene98098.t1	AT1G65620	48.077	104	54	0	6	109	10	113	5.59e-32	113
MS.gene98098.t1	AT1G65620	48.077	104	54	0	6	109	10	113	5.59e-32	113
MS.gene98098.t1	AT2G23660	48.515	101	52	0	6	106	6	106	5.71e-32	116
MS.gene98098.t1	AT2G23660	48.515	101	52	0	6	106	6	106	5.71e-32	116
MS.gene98098.t1	AT2G23660	48.515	101	52	0	6	106	6	106	5.71e-32	116
MS.gene98098.t1	AT3G11090	44.660	103	57	0	6	108	12	114	2.50e-31	110
MS.gene98098.t1	AT4G00210	37.500	144	80	3	6	139	12	155	2.03e-27	102
MS.gene98098.t1	AT2G42430	44.762	105	57	1	6	109	16	120	6.26e-27	101
MS.gene98098.t1	AT3G50510	34.641	153	84	2	2	154	9	145	6.40e-27	100
MS.gene98098.t1	AT3G50510	34.641	153	84	2	2	154	9	145	6.40e-27	100
MS.gene98098.t1	AT3G03760	44.554	101	55	1	6	105	52	152	7.47e-27	102
MS.gene98098.t1	AT2G42440	45.000	100	54	1	6	104	8	107	1.51e-25	98.2
MS.gene98098.t1	AT3G58190	44.118	102	56	1	6	106	12	113	3.26e-25	96.7
MS.gene98098.t1	AT5G06080	43.119	109	61	1	6	113	8	116	4.69e-25	95.1
MS.gene98098.t1	AT3G13850	35.714	154	91	2	6	151	37	190	4.97e-25	97.1
MS.gene98098.t1	AT4G00220	42.308	104	59	1	8	110	20	123	4.43e-24	94.0
MS.gene98098.t1	AT2G45420	42.308	104	59	1	8	110	40	143	1.51e-23	93.2
MS.gene98098.t1	AT2G45410	42.000	100	57	1	8	106	19	118	2.25e-23	91.3
MS.gene98098.t1	AT5G35900	37.624	101	63	0	6	106	6	106	6.15e-22	87.8
MS.gene98098.t1	AT3G47870	37.500	104	65	0	6	109	37	140	9.14e-22	89.7
MS.gene98098.t1	AT2G31310	34.314	102	66	1	6	106	8	109	4.03e-20	82.4
MS.gene98098.t1	AT1G06280	37.931	87	54	0	6	92	25	111	4.89e-18	77.4
MS.gene98098.t1	AT1G72980	36.000	100	64	0	6	105	14	113	7.92e-18	77.0
MS.gene98098.t1	AT2G19820	34.043	94	61	1	6	98	13	106	1.67e-15	68.9
MS.gene98098.t1	AT4G22700	33.043	115	71	3	1	110	1	114	2.00e-13	65.1
MS.gene98098.t1	AT5G15060	43.243	74	38	2	6	76	18	90	9.56e-13	62.0
MS.gene98098.t1	AT1G36000	30.682	88	61	0	4	91	8	95	2.44e-12	60.5

Find 31 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACCTTCAAATTGTTGATTT+TGG	0.080390	7.2:+71052268	None:intergenic
AGGATTCAAGATCCTGTTTA+TGG	0.232394	7.2:-71052384	MS.gene98098:CDS
GGCCTAGTCAAACTCCTAAT+TGG	0.295938	7.2:-71052155	MS.gene98098:CDS
GGATCATTGGGAGGGAAATA+TGG	0.321654	7.2:+71052890	None:intergenic
GCTTCTGTTCACCGAATCTA+TGG	0.327300	7.2:-71052857	MS.gene98098:CDS
AGCAAATCTTTGAGGATCAT+TGG	0.352548	7.2:+71052877	None:intergenic
AACTCCTAATTGGTTCAATT+AGG	0.365498	7.2:-71052145	None:intergenic
TTAACATCATGATTTATAGA+AGG	0.383363	7.2:-71052967	None:intergenic
AAGTCCTAATTGAACCAATT+AGG	0.385923	7.2:+71052141	None:intergenic
ATCCCAACACATCCATAAAC+AGG	0.432009	7.2:+71052372	None:intergenic
GCAAATCTTTGAGGATCATT+GGG	0.442273	7.2:+71052878	None:intergenic
CTCCAAAATCAACAATTTGA+AGG	0.458414	7.2:-71052270	MS.gene98098:CDS
ATCTATGGTGGTAGCAATGT+TGG	0.480363	7.2:-71052842	MS.gene98098:CDS
TGTATTTAGAAGCACAATGT+AGG	0.497154	7.2:-71052404	MS.gene98098:CDS
ATCCTGTTTATGGATGTGTT+GGG	0.518295	7.2:-71052374	MS.gene98098:CDS
TCAAGATACAGAAGTTGAGT+TGG	0.518830	7.2:-71052325	MS.gene98098:CDS
TATGGAGAGAAAATGCAATC+AGG	0.521384	7.2:+71052908	None:intergenic
ACAACATGGAGCAGATTCAA+TGG	0.528352	7.2:-71052176	MS.gene98098:CDS
CATTGCTACCACCATAGATT+CGG	0.528587	7.2:+71052846	None:intergenic
GATCCTGTTTATGGATGTGT+TGG	0.530554	7.2:-71052375	MS.gene98098:CDS
TGAACAGAAGCAAATCTTTG+AGG	0.534397	7.2:+71052869	None:intergenic
AATACATAGAATTTGCAGCT+TGG	0.550687	7.2:+71052421	None:intergenic
CTTGCAAGAGTCAAAGAAGA+AGG	0.562843	7.2:-71052937	MS.gene98098:CDS
ATCTTTGAGGATCATTGGGA+GGG	0.584186	7.2:+71052882	None:intergenic
AATCTTTGAGGATCATTGGG+AGG	0.604113	7.2:+71052881	None:intergenic
TCTGTTCACCGAATCTATGG+TGG	0.619615	7.2:-71052854	MS.gene98098:CDS
AACCAATTAGGAGTTTGACT+AGG	0.647531	7.2:+71052153	None:intergenic
TCCATGTTGTTACTATGTAG+TGG	0.647940	7.2:+71052189	None:intergenic
GCCACTACATAGTAACAACA+TGG	0.651155	7.2:-71052190	MS.gene98098:CDS
GCTTGGTGTCGTACATAGTG+AGG	0.654586	7.2:+71052438	None:intergenic
GGAGAGAAAATGCAATCAGG+AGG	0.655607	7.2:+71052911	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTTTAAAATAATCTTATTG+TGG	-	chr7.2:71052622-71052641	MS.gene98098:intron	10.0%
!!	AACATTATATAATTATAGCA+TGG	-	chr7.2:71052530-71052549	MS.gene98098:intron	15.0%
!!	TACCATGTAATATATTATTG+TGG	-	chr7.2:71052384-71052403	MS.gene98098:CDS	20.0%
!!	TCAATGTAAGAATCAATATT+TGG	-	chr7.2:71052427-71052446	MS.gene98098:CDS	20.0%
!!	TTCCACAATAATATATTACA+TGG	+	chr7.2:71052389-71052408	None:intergenic	20.0%
	AATACATAGAATTTGCAGCT+TGG	+	chr7.2:71052687-71052706	None:intergenic	30.0%
	CTCCAAAATCAACAATTTGA+AGG	-	chr7.2:71052835-71052854	MS.gene98098:CDS	30.0%
!	TGTATTTAGAAGCACAATGT+AGG	-	chr7.2:71052701-71052720	MS.gene98098:intron	30.0%
!!!	CACCTTCAAATTGTTGATTT+TGG	+	chr7.2:71052840-71052859	None:intergenic	30.0%
	AACCAATTAGGAGTTTGACT+AGG	+	chr7.2:71052955-71052974	None:intergenic	35.0%
	AGGATTCAAGATCCTGTTTA+TGG	-	chr7.2:71052721-71052740	MS.gene98098:intron	35.0%
	ATCCTGTTTATGGATGTGTT+GGG	-	chr7.2:71052731-71052750	MS.gene98098:intron	35.0%
	CAATGTTGGAAAAATGCTTC+AGG	-	chr7.2:71052277-71052296	MS.gene98098:CDS	35.0%
	TATGGAGAGAAAATGCAATC+AGG	+	chr7.2:71052200-71052219	None:intergenic	35.0%
	TCAAGATACAGAAGTTGAGT+TGG	-	chr7.2:71052780-71052799	MS.gene98098:intron	35.0%
	TCCATGTTGTTACTATGTAG+TGG	+	chr7.2:71052919-71052938	None:intergenic	35.0%
	TGAACAGAAGCAAATCTTTG+AGG	+	chr7.2:71052239-71052258	None:intergenic	35.0%
!	AGCAAATCTTTGAGGATCAT+TGG	+	chr7.2:71052231-71052250	None:intergenic	35.0%
!	GCAAATCTTTGAGGATCATT+GGG	+	chr7.2:71052230-71052249	None:intergenic	35.0%
	ACAACATGGAGCAGATTCAA+TGG	-	chr7.2:71052929-71052948	MS.gene98098:CDS	40.0%
	ATCCCAACACATCCATAAAC+AGG	+	chr7.2:71052736-71052755	None:intergenic	40.0%
	ATCTATGGTGGTAGCAATGT+TGG	-	chr7.2:71052263-71052282	MS.gene98098:CDS	40.0%
	CATTGCTACCACCATAGATT+CGG	+	chr7.2:71052262-71052281	None:intergenic	40.0%
	CTTGCAAGAGTCAAAGAAGA+AGG	-	chr7.2:71052168-71052187	MS.gene98098:CDS	40.0%
	GATCCTGTTTATGGATGTGT+TGG	-	chr7.2:71052730-71052749	MS.gene98098:intron	40.0%
	GCCACTACATAGTAACAACA+TGG	-	chr7.2:71052915-71052934	MS.gene98098:CDS	40.0%
!	AATCTTTGAGGATCATTGGG+AGG	+	chr7.2:71052227-71052246	None:intergenic	40.0%
!	ATCTTTGAGGATCATTGGGA+GGG	+	chr7.2:71052226-71052245	None:intergenic	40.0%
	GCTTCTGTTCACCGAATCTA+TGG	-	chr7.2:71052248-71052267	MS.gene98098:CDS	45.0%
	GGAGAGAAAATGCAATCAGG+AGG	+	chr7.2:71052197-71052216	None:intergenic	45.0%
	GGATCATTGGGAGGGAAATA+TGG	+	chr7.2:71052218-71052237	None:intergenic	45.0%
	GGCCTAGTCAAACTCCTAAT+TGG	-	chr7.2:71052950-71052969	MS.gene98098:CDS	45.0%
	TCTGTTCACCGAATCTATGG+TGG	-	chr7.2:71052251-71052270	MS.gene98098:CDS	45.0%
!!	GCTTGGTGTCGTACATAGTG+AGG	+	chr7.2:71052670-71052689	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.2	gene	71052146	71052981	71052146	ID=MS.gene98098
chr7.2	mRNA	71052146	71052981	71052146	ID=MS.gene98098.t1;Parent=MS.gene98098
chr7.2	exon	71052829	71052981	71052829	ID=MS.gene98098.t1.exon1;Parent=MS.gene98098.t1
chr7.2	CDS	71052829	71052981	71052829	ID=cds.MS.gene98098.t1;Parent=MS.gene98098.t1
chr7.2	exon	71052146	71052466	71052146	ID=MS.gene98098.t1.exon2;Parent=MS.gene98098.t1
chr7.2	CDS	71052146	71052466	71052146	ID=cds.MS.gene98098.t1;Parent=MS.gene98098.t1

Gene Sequence

>MS.gene98098

ATGATTTATAGAAGGTGTGCTGCTTGCAAGAGTCAAAGAAGAAGGTGTCCTCCTGATTGCATTTTCTCTCCATATTTCCCTCCCAATGATCCTCAAAGATTTGCTTCTGTTCACCGAATCTATGGTGGTAGCAATGTTGGAAAAATGCTTCAGGTACTCTTAATAATATAATAAATATATCAATTCAATATTTAGTTTTTTTTTTTGAAATAATCAATATTTAGTATCTAGTATGTATTACCATGTAATATATTATTGTGGAATTTACTTGTAATTTTGTGTCAATGTAAGAATCAATATTTGGTATCTAGATTTCATTAGTGTACTTTTAACTTATAAATTATCAATATCAATATTTAGTATCTAGTGTATGTATTAGTATGTAACATTATATAATTATAGCATGGACATAATATAAGAGTTAGAAAAAGTATACTTTTTACTTATAGTTTTTTTTTTTTAATGAAAATTTGTATATTTTAAAATAATCTTATTGTGGAATTTTAATGTTGTAGCAATTGCCTCACTATGTACGACACCAAGCTGCAAATTCTATGTATTTAGAAGCACAATGTAGGATTCAAGATCCTGTTTATGGATGTGTTGGGATTATCTCTAAATTATCTCAACAAATTCAAGATACAGAAGTTGAGTTGGCTAAAATCAAAACTCAGATTGCTTATCATAAACTCCAAAATCAACAATTTGAAGGTGAATCAAACTTCAATGTCTTGTCAACAATTGATGCTGAATCAAACTTAAGCTCTTTGCCACTACATAGTAACAACATGGAGCAGATTCAATGGCCTAGTCAAACTCCTAATTGGTTCAATTAG

Protein sequence

>MS.gene98098.t1

MIYRRCAACKSQRRRCPPDCIFSPYFPPNDPQRFASVHRIYGGSNVGKMLQQLPHYVRHQAANSMYLEAQCRIQDPVYGCVGIISKLSQQIQDTEVELAKIKTQIAYHKLQNQQFEGESNFNVLSTIDAESNLSSLPLHSNNMEQIQWPSQTPNWFN