AlfalfaGEDB | MS.gene98418

M. sativa cultivar XinJiangDaYe / MS.gene98418

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98418.t1	KEH26852.1	51.9	81	39	0	10	90	64	144	6.60E-14	86.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98418.t1	A0A072UB72	51.9	81	39	0	10	90	64	144	4.8e-14	86.7

TFs/TRs:

Gene ID	Type	Classification
MS.gene98418.t1	TF	B3

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98418.t1	MTR_6g477950	51.852	81	39	0	10	90	64	144	6.10e-22	87.4
MS.gene98418.t1	MTR_7g117585	36.667	90	57	0	1	90	56	145	4.53e-17	71.2
MS.gene98418.t1	MTR_2g062330	41.667	84	48	1	7	90	56	138	1.04e-16	70.5
MS.gene98418.t1	MTR_5g071410	40.000	90	54	0	1	90	179	268	1.08e-16	72.8
MS.gene98418.t1	MTR_8g069780	38.750	80	49	0	11	90	76	155	2.56e-16	69.7
MS.gene98418.t1	MTR_2g012700	36.471	85	54	0	7	91	36	120	7.83e-14	62.4
MS.gene98418.t1	MTR_3g117130	42.029	69	40	0	9	77	197	265	1.08e-13	64.7
MS.gene98418.t1	MTR_6g034285	66.667	36	12	0	38	73	365	400	1.62e-11	58.9
MS.gene98418.t1	MTR_6g018740	44.828	58	32	0	11	68	52	109	2.65e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 22 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTGCAGGAGGACGACAAAT+TGG	0.369744	7.4:-79015809	MS.gene98418:CDS
CTAATTGAGGTAAGACTTCT+CGG	0.383272	7.4:+79015959	None:intergenic
TCATGCTAATGACCTTGATT+AGG	0.396314	7.4:-79015740	None:intergenic
AACAAAGCTGAGAAATCTTT+AGG	0.397658	7.4:-79015865	MS.gene98418:CDS
TGCGCTTGGTAGTAATGATT+TGG	0.410935	7.4:+79015887	None:intergenic
CGAGAAGTCTTACCTCAATT+AGG	0.419615	7.4:-79015958	MS.gene98418:CDS
GTTATCTTGTTGCCTAATTG+AGG	0.443207	7.4:+79015946	None:intergenic
TGTGAATCTTTGCCTAATCA+AGG	0.458326	7.4:+79015728	None:intergenic
GCTGAGAAATCTTTAGGAGC+AGG	0.472784	7.4:-79015859	MS.gene98418:CDS
GCCATCTGATGTATCAAGTA+AGG	0.478299	7.4:+79015924	None:intergenic
TTTCTCAGCTTTGTTGCGCT+TGG	0.512478	7.4:+79015873	None:intergenic
TGTAAATGAGAAAGAGCTGC+AGG	0.520669	7.4:-79015824	MS.gene98418:CDS
GTAAGACTTCTCGGGATACA+CGG	0.550609	7.4:+79015968	None:intergenic
CACTAATAAACAGCAAGTAA+TGG	0.558500	7.4:-79016130	MS.gene98418:intron
CTAACACTGTAGGAGGATCA+AGG	0.570132	7.4:+79015776	None:intergenic
ACGTCAACTGCTAACACTGT+AGG	0.578213	7.4:+79015766	None:intergenic
TCAACTGCTAACACTGTAGG+AGG	0.585589	7.4:+79015769	None:intergenic
AGAAATCTTTAGGAGCAGGT+TGG	0.589032	7.4:-79015855	MS.gene98418:CDS
TAATTGAGGTAAGACTTCTC+GGG	0.590285	7.4:+79015960	None:intergenic
ACCTTACTTGATACATCAGA+TGG	0.593255	7.4:-79015925	MS.gene98418:CDS
AAATGAGAAAGAGCTGCAGG+AGG	0.711877	7.4:-79015821	MS.gene98418:CDS
ACTTCTCGGGATACACGGCG+TGG	0.721677	7.4:+79015973	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATAATGATTATATTTCTT+TGG	-	chr7.4:79015851-79015870	MS.gene98418:CDS	10.0%
	AACAAAGCTGAGAAATCTTT+AGG	-	chr7.4:79016014-79016033	MS.gene98418:intron	30.0%
	CACTAATAAACAGCAAGTAA+TGG	-	chr7.4:79015749-79015768	MS.gene98418:CDS	30.0%
	ACCTTACTTGATACATCAGA+TGG	-	chr7.4:79015954-79015973	MS.gene98418:CDS	35.0%
	CTAATTGAGGTAAGACTTCT+CGG	+	chr7.4:79015923-79015942	None:intergenic	35.0%
	GTTATCTTGTTGCCTAATTG+AGG	+	chr7.4:79015936-79015955	None:intergenic	35.0%
	TAATTGAGGTAAGACTTCTC+GGG	+	chr7.4:79015922-79015941	None:intergenic	35.0%
	CGAGAAGTCTTACCTCAATT+AGG	-	chr7.4:79015921-79015940	MS.gene98418:CDS	40.0%
	GCCATCTGATGTATCAAGTA+AGG	+	chr7.4:79015958-79015977	None:intergenic	40.0%
	TGTAAATGAGAAAGAGCTGC+AGG	-	chr7.4:79016055-79016074	MS.gene98418:intron	40.0%
!	AGAAATCTTTAGGAGCAGGT+TGG	-	chr7.4:79016024-79016043	MS.gene98418:intron	40.0%
!	TGCGCTTGGTAGTAATGATT+TGG	+	chr7.4:79015995-79016014	None:intergenic	40.0%
	AAATGAGAAAGAGCTGCAGG+AGG	-	chr7.4:79016058-79016077	MS.gene98418:intron	45.0%
	ACGTCAACTGCTAACACTGT+AGG	+	chr7.4:79016116-79016135	None:intergenic	45.0%
	CTAACACTGTAGGAGGATCA+AGG	+	chr7.4:79016106-79016125	None:intergenic	45.0%
	GTAAGACTTCTCGGGATACA+CGG	+	chr7.4:79015914-79015933	None:intergenic	45.0%
	TCAACTGCTAACACTGTAGG+AGG	+	chr7.4:79016113-79016132	None:intergenic	45.0%
!	GCTGAGAAATCTTTAGGAGC+AGG	-	chr7.4:79016020-79016039	MS.gene98418:intron	45.0%
!!	TTTCTCAGCTTTGTTGCGCT+TGG	+	chr7.4:79016009-79016028	None:intergenic	45.0%
	GCTGCAGGAGGACGACAAAT+TGG	-	chr7.4:79016070-79016089	MS.gene98418:intron	55.0%
	ACTTCTCGGGATACACGGCG+TGG	+	chr7.4:79015909-79015928	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	79015741	79016160	79015741	ID=MS.gene98418
chr7.4	mRNA	79015741	79016160	79015741	ID=MS.gene98418.t1;Parent=MS.gene98418
chr7.4	exon	79016131	79016160	79016131	ID=MS.gene98418.t1.exon1;Parent=MS.gene98418.t1
chr7.4	CDS	79016131	79016160	79016131	ID=cds.MS.gene98418.t1;Parent=MS.gene98418.t1
chr7.4	exon	79015741	79016001	79015741	ID=MS.gene98418.t1.exon2;Parent=MS.gene98418.t1
chr7.4	CDS	79015741	79016001	79015741	ID=cds.MS.gene98418.t1;Parent=MS.gene98418.t1

Gene Sequence

>MS.gene98418

ATGGCTGACACTAATAAACAGCAAGTAATGGTTTGTTTACATCATTAACTAATTAAAAATATATATTCTCTTCTTGTTTTTTCTACAATTTATTTTTCACTTCAAGTTTCATATAATGATTATATTTCTTTGGAATTTTATATAATTTCTTTGTAATAGCACTTTCCACGCCGTGTATCCCGAGAAGTCTTACCTCAATTAGGCAACAAGATAACCTTACTTGATACATCAGATGGCTATGAATATGATTGCCAAATCATTACTACCAAGCGCAACAAAGCTGAGAAATCTTTAGGAGCAGGTTGGTATAAGTTTGTAAATGAGAAAGAGCTGCAGGAGGACGACAAATTGGTATTTGATTTCCTTGATCCTCCTACAGTGTTAGCAGTTGACGTAATTCATGCTAATGACCTTGATTAG

Protein sequence

>MS.gene98418.t1

MADTNKQQVMHFPRRVSREVLPQLGNKITLLDTSDGYEYDCQIITTKRNKAEKSLGAGWYKFVNEKELQEDDKLVFDFLDPPTVLAVDVIHANDLD