Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98572.t1 | KEH40705.1 | 92.5 | 93 | 7 | 0 | 1 | 93 | 1 | 93 | 9.00E-41 | 176 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98572.t1 | Q9FNN6 | 60.9 | 87 | 33 | 1 | 1 | 87 | 1 | 86 | 1.9e-23 | 109.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98572.t1 | A0A072VRE4 | 92.5 | 93 | 7 | 0 | 1 | 93 | 1 | 93 | 6.5e-41 | 176.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene98572.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98572.t1 | MTR_1g033600 | 95.506 | 89 | 4 | 0 | 1 | 89 | 1 | 89 | 3.13e-58 | 176 |
| MS.gene98572.t1 | MTR_8g063600 | 59.615 | 104 | 40 | 1 | 3 | 104 | 1 | 104 | 7.03e-36 | 123 |
| MS.gene98572.t1 | MTR_8g063870 | 62.500 | 88 | 31 | 1 | 3 | 90 | 1 | 86 | 6.03e-30 | 107 |
| MS.gene98572.t1 | MTR_1g022290 | 51.807 | 83 | 40 | 0 | 6 | 88 | 1 | 83 | 3.22e-26 | 93.6 |
| MS.gene98572.t1 | MTR_3g111880 | 55.072 | 69 | 31 | 0 | 9 | 77 | 4 | 72 | 4.23e-22 | 82.8 |
| MS.gene98572.t1 | MTR_5g020170 | 48.052 | 77 | 40 | 0 | 7 | 83 | 14 | 90 | 4.41e-21 | 80.5 |
| MS.gene98572.t1 | MTR_3g116720 | 50.000 | 68 | 34 | 0 | 10 | 77 | 14 | 81 | 5.41e-21 | 79.7 |
| MS.gene98572.t1 | MTR_7g089210 | 56.250 | 64 | 28 | 0 | 15 | 78 | 15 | 78 | 1.45e-20 | 79.0 |
| MS.gene98572.t1 | MTR_6g004250 | 50.000 | 74 | 37 | 0 | 3 | 76 | 1 | 74 | 3.95e-20 | 77.8 |
| MS.gene98572.t1 | MTR_4g111975 | 56.061 | 66 | 26 | 1 | 12 | 74 | 8 | 73 | 5.86e-20 | 82.4 |
| MS.gene98572.t1 | MTR_1g111830 | 53.623 | 69 | 32 | 0 | 10 | 78 | 22 | 90 | 5.73e-19 | 78.6 |
| MS.gene98572.t1 | MTR_5g081860 | 52.174 | 69 | 33 | 0 | 10 | 78 | 25 | 93 | 1.25e-18 | 78.2 |
| MS.gene98572.t1 | MTR_3g462790 | 41.333 | 75 | 44 | 0 | 4 | 78 | 35 | 109 | 6.26e-18 | 77.0 |
| MS.gene98572.t1 | MTR_7g067080 | 45.833 | 72 | 39 | 0 | 7 | 78 | 30 | 101 | 1.04e-17 | 76.3 |
| MS.gene98572.t1 | MTR_5g069710 | 44.286 | 70 | 39 | 0 | 9 | 78 | 26 | 95 | 1.30e-17 | 75.9 |
| MS.gene98572.t1 | MTR_3g111920 | 36.709 | 79 | 50 | 0 | 12 | 90 | 3 | 81 | 2.02e-17 | 70.9 |
| MS.gene98572.t1 | MTR_8g077360 | 39.726 | 73 | 43 | 1 | 9 | 81 | 2 | 73 | 8.36e-17 | 68.9 |
| MS.gene98572.t1 | MTR_3g013440 | 44.000 | 75 | 41 | 1 | 3 | 76 | 1 | 75 | 2.09e-16 | 68.6 |
| MS.gene98572.t1 | MTR_8g077380 | 40.000 | 70 | 41 | 1 | 9 | 78 | 2 | 70 | 2.85e-16 | 67.8 |
| MS.gene98572.t1 | MTR_8g077390 | 40.000 | 70 | 41 | 1 | 9 | 78 | 2 | 70 | 2.85e-16 | 67.8 |
| MS.gene98572.t1 | MTR_8g077420 | 42.857 | 63 | 35 | 1 | 10 | 72 | 3 | 64 | 4.56e-14 | 62.0 |
| MS.gene98572.t1 | MTR_5g020540 | 46.032 | 63 | 33 | 1 | 10 | 72 | 2 | 63 | 6.90e-14 | 61.2 |
| MS.gene98572.t1 | MTR_1g086180 | 45.614 | 57 | 30 | 1 | 12 | 67 | 11 | 67 | 3.19e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98572.t1 | AT5G08520 | 58.947 | 95 | 38 | 1 | 1 | 95 | 1 | 94 | 1.30e-33 | 118 |
| MS.gene98572.t1 | AT4G39250 | 53.247 | 77 | 36 | 0 | 12 | 88 | 14 | 90 | 6.16e-25 | 90.5 |
| MS.gene98572.t1 | AT1G75250 | 50.000 | 74 | 37 | 0 | 10 | 83 | 10 | 83 | 1.68e-22 | 84.3 |
| MS.gene98572.t1 | AT1G75250 | 50.000 | 74 | 37 | 0 | 10 | 83 | 10 | 83 | 3.68e-22 | 84.3 |
| MS.gene98572.t1 | AT2G21650 | 51.429 | 70 | 34 | 0 | 12 | 81 | 14 | 83 | 7.66e-20 | 77.4 |
| MS.gene98572.t1 | AT1G19510 | 45.946 | 74 | 40 | 0 | 15 | 88 | 15 | 88 | 1.29e-19 | 77.0 |
| MS.gene98572.t1 | AT2G18328 | 51.562 | 64 | 31 | 0 | 15 | 78 | 14 | 77 | 6.15e-19 | 74.7 |
| MS.gene98572.t1 | AT2G38090 | 45.833 | 72 | 39 | 0 | 7 | 78 | 24 | 95 | 7.56e-18 | 76.6 |
| MS.gene98572.t1 | AT5G05790 | 50.000 | 68 | 34 | 0 | 9 | 76 | 28 | 95 | 9.70e-18 | 75.9 |
| MS.gene98572.t1 | AT5G05790 | 50.000 | 68 | 34 | 0 | 9 | 76 | 28 | 95 | 9.70e-18 | 75.9 |
| MS.gene98572.t1 | AT5G23650 | 50.000 | 70 | 35 | 0 | 5 | 74 | 6 | 75 | 1.60e-17 | 76.3 |
| MS.gene98572.t1 | AT5G58900 | 47.059 | 68 | 36 | 0 | 10 | 77 | 32 | 99 | 1.78e-17 | 75.5 |
| MS.gene98572.t1 | AT3G11280 | 47.761 | 67 | 35 | 0 | 12 | 78 | 33 | 99 | 1.70e-16 | 72.4 |
| MS.gene98572.t1 | AT3G11280 | 47.761 | 67 | 35 | 0 | 12 | 78 | 33 | 99 | 1.70e-16 | 72.4 |
| MS.gene98572.t1 | AT1G49010 | 46.377 | 69 | 34 | 1 | 10 | 75 | 6 | 74 | 1.99e-15 | 70.1 |
| MS.gene98572.t1 | AT5G04760 | 45.455 | 66 | 35 | 1 | 9 | 74 | 3 | 67 | 1.80e-14 | 66.2 |
| MS.gene98572.t1 | AT5G01200 | 43.939 | 66 | 36 | 1 | 10 | 74 | 28 | 93 | 3.37e-12 | 60.8 |
Find 0 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ACCATCGTATAATTCTTCTT+CGG | - | 11125:329-348 | MS.gene98572:CDS | 30.0% | |
| GAAGAATTATACGATGGTAA+AGG | + | 11125:327-346 | MS.gene98572:intergenic | 30.0% | |
| ATCGTATAATTCTTCTTCGG+AGG | - | 11125:332-351 | MS.gene98572:CDS | 35.0% | |
| TCCGAAGAAGAATTATACGA+TGG | + | 11125:333-352 | MS.gene98572:intergenic | 35.0% | |
| TCGTATAATTCTTCTTCGGA+GGG | - | 11125:333-352 | MS.gene98572:CDS | 35.0% | |
| ! | TAAGGCATTTGAAAATGCCT+TGG | - | 11125:167-186 | MS.gene98572:CDS | 35.0% |
| GACTTATCCTGAGGATGATT+CGG | - | 11125:191-210 | MS.gene98572:CDS | 40.0% | |
| GATATTAGCCAGATTGAAGC+TGG | - | 11125:294-313 | MS.gene98572:CDS | 40.0% | |
| TAAGCAGCATTATGAGCTCT+TGG | - | 11125:266-285 | MS.gene98572:CDS | 40.0% | |
| ACAAGTCATGCTAGCGATGA+AGG | - | 11125:360-379 | MS.gene98572:CDS | 45.0% | |
| ATTTCTTCCGTAGTCTTCCC+CGG | + | 11125:246-265 | MS.gene98572:intergenic | 45.0% | |
| CAAGTCATGCTAGCGATGAA+GGG | - | 11125:361-380 | MS.gene98572:CDS | 45.0% | |
| ! | TGAGGATGATTCGGATCGAT+GGG | - | 11125:200-219 | MS.gene98572:CDS | 45.0% |
| !! | AAGTGGACAATAGCTCTGAG+TGG | - | 11125:130-149 | MS.gene98572:CDS | 45.0% |
| ATCCTCAGGATAAGTCGCCA+AGG | + | 11125:187-206 | MS.gene98572:intergenic | 50.0% | |
| ATGCTAGCGATGAAGGGAGT+TGG | - | 11125:367-386 | MS.gene98572:CDS | 50.0% | |
| CATCGATCCGAATCATCCTC+AGG | + | 11125:201-220 | MS.gene98572:intergenic | 50.0% | |
| GCACACAACCAGCTTCAATC+TGG | + | 11125:305-324 | MS.gene98572:intergenic | 50.0% | |
| GGATCGATGGGAGAAGATTG+CGG | - | 11125:212-231 | MS.gene98572:CDS | 50.0% | |
| TGCTAGCGATGAAGGGAGTT+GGG | - | 11125:368-387 | MS.gene98572:CDS | 50.0% | |
| ! | CTGAGGATGATTCGGATCGA+TGG | - | 11125:199-218 | MS.gene98572:CDS | 50.0% |
| !! | ACAATAGCTCTGAGTGGAGC+TGG | - | 11125:136-155 | MS.gene98572:CDS | 50.0% |
| !! | CAATAGCTCTGAGTGGAGCT+GGG | - | 11125:137-156 | MS.gene98572:CDS | 50.0% |
| AAGATTGCGGCCGATGTACC+GGG | - | 11125:225-244 | MS.gene98572:CDS | 55.0% | |
| AAGGGAGTTGGGGGGAAGAA+GGG | - | 11125:379-398 | MS.gene98572:CDS | 55.0% | |
| CGATGTACCGGGGAAGACTA+CGG | - | 11125:236-255 | MS.gene98572:CDS | 55.0% | |
| CGTAGTCTTCCCCGGTACAT+CGG | + | 11125:238-257 | MS.gene98572:intergenic | 55.0% | |
| CTAGCGATGAAGGGAGTTGG+GGG | - | 11125:370-389 | MS.gene98572:CDS | 55.0% | |
| GAAGATTGCGGCCGATGTAC+CGG | - | 11125:224-243 | MS.gene98572:CDS | 55.0% | |
| GCTAGCGATGAAGGGAGTTG+GGG | - | 11125:369-388 | MS.gene98572:CDS | 55.0% | |
| GGGAAGAAGGGTGGTCACAA+TGG | - | 11125:391-410 | MS.gene98572:CDS | 55.0% | |
| GTGGAGCTGGGAGCAAGATA+AGG | - | 11125:149-168 | MS.gene98572:CDS | 55.0% | |
| TAGCGATGAAGGGAGTTGGG+GGG | - | 11125:371-390 | MS.gene98572:CDS | 55.0% | |
| TGCCTTGGCGACTTATCCTG+AGG | - | 11125:182-201 | MS.gene98572:CDS | 55.0% | |
| AGATTGCGGCCGATGTACCG+GGG | - | 11125:226-245 | MS.gene98572:CDS | 60.0% | |
| GAAGGGAGTTGGGGGGAAGA+AGG | - | 11125:378-397 | MS.gene98572:CDS | 60.0% | |
| GGAGTTGGGGGGAAGAAGGG+TGG | - | 11125:382-401 | MS.gene98572:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11125 | gene | 117 | 434 | 117 | ID=MS.gene98572 |
| 11125 | mRNA | 117 | 434 | 117 | ID=MS.gene98572.t1;Parent=MS.gene98572 |
| 11125 | exon | 117 | 434 | 117 | ID=MS.gene98572.t1.exon1;Parent=MS.gene98572.t1 |
| 11125 | CDS | 117 | 434 | 117 | ID=cds.MS.gene98572.t1;Parent=MS.gene98572.t1 |
| Gene Sequence |
| Protein sequence |
