AlfalfaGEDB | MS.gene98674

M. sativa cultivar XinJiangDaYe / MS.gene98674

KEGG: -
GO: -
PFAM: PF04770.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98674.t1	KEH23232.1	89.4	85	9	0	31	115	54	138	2.00E-37	165.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98674.t1	Q9SB61	47.8	90	40	2	8	97	5	87	7.6e-14	78.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98674.t1	A0A072U0M5	89.4	85	9	0	31	115	54	138	1.4e-37	165.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene98674.t1	TF	zf-HD

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98674.t1	MTR_7g070643	82.653	98	14	1	22	119	48	142	2.10e-55	171
MS.gene98674.t1	MTR_5g065440	88.660	97	10	1	1	97	1	96	5.04e-45	147
MS.gene98674.t1	MTR_6g080300	83.607	61	10	0	37	97	32	92	3.35e-32	109
MS.gene98674.t1	MTR_2g007810	84.211	57	9	0	63	119	13	69	1.54e-29	102
MS.gene98674.t1	MTR_3g100650	62.791	86	23	2	20	97	34	118	1.08e-24	95.5
MS.gene98674.t1	MTR_5g032600	70.732	41	12	0	57	97	76	116	7.11e-14	66.6
MS.gene98674.t1	MTR_3g039810	65.854	41	14	0	57	97	18	58	2.45e-12	58.9
MS.gene98674.t1	MTR_1g077020	60.976	41	16	0	57	97	7	47	1.43e-11	59.3
MS.gene98674.t1	MTR_4g134580	62.500	40	15	0	58	97	112	151	1.56e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene98674.t1	AT4G24660	48.889	90	39	2	8	97	5	87	6.60e-17	73.9
MS.gene98674.t1	AT4G24660	48.889	90	39	2	8	97	5	87	9.08e-17	73.6
MS.gene98674.t1	AT3G50890	75.610	41	10	0	57	97	58	98	4.96e-16	72.0
MS.gene98674.t1	AT1G74660	70.455	44	13	0	54	97	34	77	2.59e-15	67.0
MS.gene98674.t1	AT2G18350	70.732	41	12	0	57	97	80	120	2.64e-15	70.1
MS.gene98674.t1	AT1G74660	70.455	44	13	0	54	97	34	77	2.75e-15	67.0
MS.gene98674.t1	AT3G28917	57.143	63	26	1	54	115	28	90	2.10e-14	64.7
MS.gene98674.t1	AT5G65410	62.500	48	18	0	50	97	66	113	5.55e-13	64.3
MS.gene98674.t1	AT1G75240	69.048	42	13	0	56	97	73	114	7.00e-13	63.9
MS.gene98674.t1	AT2G02540	57.500	40	17	0	57	96	85	124	2.56e-12	62.4
MS.gene98674.t1	AT2G02540	57.500	40	17	0	57	96	85	124	2.56e-12	62.4
MS.gene98674.t1	AT1G14440	56.098	41	18	0	57	97	88	128	7.76e-12	61.2
MS.gene98674.t1	AT1G14440	56.098	41	18	0	57	97	88	128	7.76e-12	61.2

Find 24 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGACGGTTGTTGCGAGTTTA+TGG	0.243395	7.1:-31663743	MS.gene98674:CDS
CGTGATTCTTCTGACATTCT+TGG	0.260175	7.1:+31663791	None:intergenic
GCTTGGAGAATTCAGAATTC+TGG	0.289840	7.1:-31663679	MS.gene98674:CDS
TTCGGTGGCCACGCTGTTGA+CGG	0.414354	7.1:-31663760	MS.gene98674:CDS
AAGCTGTTATTTGCGCTGCT+TGG	0.453807	7.1:-31663696	MS.gene98674:CDS
TTTATGGCTGCCGGTGAAGA+AGG	0.464102	7.1:-31663727	MS.gene98674:CDS
CGAATCAGATTCGCTTATGT+TGG	0.484764	7.1:-31663600	MS.gene98674:CDS
AATGGAATTTGATGAACAAA+CGG	0.488408	7.1:-31663968	MS.gene98674:CDS
AAGAATCACGCTGTTAGCTT+CGG	0.499674	7.1:-31663778	MS.gene98674:CDS
TTCAAGTGTTCCTTCTTCAC+CGG	0.506613	7.1:+31663717	None:intergenic
AAACTCACCATCGTAGTTGA+CGG	0.531063	7.1:-31663859	MS.gene98674:CDS
AACGGAACAAGAAGAGGAGA+TGG	0.536127	7.1:-31663950	MS.gene98674:CDS
GAGTCATAACTCGTTGGCAC+TGG	0.537593	7.1:+31663907	None:intergenic
AATCACGCTGTTAGCTTCGG+TGG	0.563601	7.1:-31663775	MS.gene98674:CDS
ATCGTAGTTGACGGAAGGAA+AGG	0.583477	7.1:-31663850	MS.gene98674:CDS
TGAACAAACGGAACAAGAAG+AGG	0.590353	7.1:-31663956	MS.gene98674:CDS
CCAACGAGTTATGACTCACT+TGG	0.611073	7.1:-31663901	MS.gene98674:CDS
TGTTGCGAGTTTATGGCTGC+CGG	0.619539	7.1:-31663736	MS.gene98674:CDS
TTTCCTTCCGTCAACTACGA+TGG	0.621872	7.1:+31663852	None:intergenic
TCACCATCGTAGTTGACGGA+AGG	0.639038	7.1:-31663855	MS.gene98674:CDS
CCAAGTGAGTCATAACTCGT+TGG	0.644449	7.1:+31663901	None:intergenic
CGCAACAACCGTCAACAGCG+TGG	0.700015	7.1:+31663752	None:intergenic
GGAAGGAAAGGTAACGACAA+CGG	0.717401	7.1:-31663838	MS.gene98674:CDS
AGGAAAGGTAACGACAACGG+CGG	0.798323	7.1:-31663835	MS.gene98674:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AACAACACAAAAACAGGAAG+GGG	+	chr7.1:31663928-31663947	None:intergenic	35.0%
	ACAACAACACAAAAACAGGA+AGG	+	chr7.1:31663930-31663949	None:intergenic	35.0%
	CAACAACACAAAAACAGGAA+GGG	+	chr7.1:31663929-31663948	None:intergenic	35.0%
!!!	TTCCTGTTTTTGTGTTGTTG+TGG	-	chr7.1:31663929-31663948	MS.gene98674:CDS	35.0%
	AAACTCACCATCGTAGTTGA+CGG	-	chr7.1:31663705-31663724	MS.gene98674:CDS	40.0%
	ACAACACAAAAACAGGAAGG+GGG	+	chr7.1:31663927-31663946	None:intergenic	40.0%
	AGGAAGGGGGAATAAGAATT+CGG	+	chr7.1:31663914-31663933	None:intergenic	40.0%
	CACCACAACAACACAAAAAC+AGG	+	chr7.1:31663934-31663953	None:intergenic	40.0%
	CGAATCAGATTCGCTTATGT+TGG	-	chr7.1:31663964-31663983	MS.gene98674:CDS	40.0%
	CGTGATTCTTCTGACATTCT+TGG	+	chr7.1:31663776-31663795	None:intergenic	40.0%
	GCTTGGAGAATTCAGAATTC+TGG	-	chr7.1:31663885-31663904	MS.gene98674:CDS	40.0%
	TGAACAAACGGAACAAGAAG+AGG	-	chr7.1:31663608-31663627	MS.gene98674:CDS	40.0%
	TTCAAGTGTTCCTTCTTCAC+CGG	+	chr7.1:31663850-31663869	None:intergenic	40.0%
!	AAGAATCACGCTGTTAGCTT+CGG	-	chr7.1:31663786-31663805	MS.gene98674:CDS	40.0%
!!!	CTGTTTTTGTGTTGTTGTGG+TGG	-	chr7.1:31663932-31663951	MS.gene98674:CDS	40.0%
	AACGGAACAAGAAGAGGAGA+TGG	-	chr7.1:31663614-31663633	MS.gene98674:CDS	45.0%
	ATCGTAGTTGACGGAAGGAA+AGG	-	chr7.1:31663714-31663733	MS.gene98674:CDS	45.0%
	CCAACGAGTTATGACTCACT+TGG	-	chr7.1:31663663-31663682	MS.gene98674:CDS	45.0%
	CCAAGTGAGTCATAACTCGT+TGG	+	chr7.1:31663666-31663685	None:intergenic	45.0%
	GGAAGGAAAGGTAACGACAA+CGG	-	chr7.1:31663726-31663745	MS.gene98674:CDS	45.0%
	TGACGGTTGTTGCGAGTTTA+TGG	-	chr7.1:31663821-31663840	MS.gene98674:CDS	45.0%
	TTTCCTTCCGTCAACTACGA+TGG	+	chr7.1:31663715-31663734	None:intergenic	45.0%
!	AAGCTGTTATTTGCGCTGCT+TGG	-	chr7.1:31663868-31663887	MS.gene98674:CDS	45.0%
!!!	GGCACTGGTGTTTGTGTTTT+TGG	+	chr7.1:31663645-31663664	None:intergenic	45.0%
	AGGAAAGGTAACGACAACGG+CGG	-	chr7.1:31663729-31663748	MS.gene98674:CDS	50.0%
	GAGTCATAACTCGTTGGCAC+TGG	+	chr7.1:31663660-31663679	None:intergenic	50.0%
	TCACCATCGTAGTTGACGGA+AGG	-	chr7.1:31663709-31663728	MS.gene98674:CDS	50.0%
	TGTTGCGAGTTTATGGCTGC+CGG	-	chr7.1:31663828-31663847	MS.gene98674:CDS	50.0%
	TTTATGGCTGCCGGTGAAGA+AGG	-	chr7.1:31663837-31663856	MS.gene98674:CDS	50.0%
!	AATCACGCTGTTAGCTTCGG+TGG	-	chr7.1:31663789-31663808	MS.gene98674:CDS	50.0%
!!!	CAACGGCGGTTTTGTTTCGA+CGG	-	chr7.1:31663743-31663762	MS.gene98674:CDS	50.0%
!!!	GCGGTTTTGTTTCGACGGTG+AGG	-	chr7.1:31663748-31663767	MS.gene98674:CDS	55.0%
	CGCAACAACCGTCAACAGCG+TGG	+	chr7.1:31663815-31663834	None:intergenic	60.0%
	TTCGGTGGCCACGCTGTTGA+CGG	-	chr7.1:31663804-31663823	MS.gene98674:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	31663597	31663989	31663597	ID=MS.gene98674
chr7.1	mRNA	31663597	31663989	31663597	ID=MS.gene98674.t1;Parent=MS.gene98674
chr7.1	exon	31663597	31663989	31663597	ID=MS.gene98674.t1.exon1;Parent=MS.gene98674.t1
chr7.1	CDS	31663597	31663989	31663597	ID=cds.MS.gene98674.t1;Parent=MS.gene98674.t1

Gene Sequence

>MS.gene98674

ATGGAATTTGATGAACAAACGGAACAAGAAGAGGAGATGGAAATTCCAAAAACACAAACACCAGTGCCAACGAGTTATGACTCACTTGGTAACTCAGCTTCACGTTCAAAACTCACCATCGTAGTTGACGGAAGGAAAGGTAACGACAACGGCGGTTTTGTTTCGACGGTGAGGTACCAAGAATGTCAGAAGAATCACGCTGTTAGCTTCGGTGGCCACGCTGTTGACGGTTGTTGCGAGTTTATGGCTGCCGGTGAAGAAGGAACACTTGAAGCTGTTATTTGCGCTGCTTGGAGAATTCAGAATTCTGGAATCCGAATTCTTATTCCCCCTTCCTGTTTTTGTGTTGTTGTGGTGGTGTGTTGCACGAATCAGATTCGCTTATGTTGGTGA

Protein sequence

>MS.gene98674.t1

MEFDEQTEQEEEMEIPKTQTPVPTSYDSLGNSASRSKLTIVVDGRKGNDNGGFVSTVRYQECQKNHAVSFGGHAVDGCCEFMAAGEEGTLEAVICAAWRIQNSGIRILIPPSCFCVVVVVCCTNQIRLCW