AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99021

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99021.t1 MTR_5g038280 94.895 333 8 1 1 333 1 324 0.0 643
MS.gene99021.t1 MTR_8g469430 53.030 330 94 8 10 333 12 286 2.93e-97 289
MS.gene99021.t1 MTR_4g107650 47.293 351 151 10 1 333 1 335 5.17e-79 244
MS.gene99021.t1 MTR_6g007647 47.208 197 90 3 87 280 33 218 1.30e-42 148
MS.gene99021.t1 MTR_6g007647 47.208 197 90 3 87 280 67 252 1.79e-42 149
MS.gene99021.t1 MTR_6g011610 43.388 242 95 10 39 261 41 259 1.23e-39 141
MS.gene99021.t1 MTR_6g011610 47.980 198 76 7 74 261 60 240 1.71e-39 140
MS.gene99021.t1 MTR_8g089895 64.815 108 37 1 77 183 43 150 6.38e-39 140
MS.gene99021.t1 MTR_3g086790 60.526 114 41 1 70 183 47 156 6.52e-38 137
MS.gene99021.t1 MTR_3g080100 66.327 98 33 0 87 184 20 117 2.14e-37 133
MS.gene99021.t1 MTR_3g080100 66.327 98 33 0 87 184 59 156 3.51e-37 134
MS.gene99021.t1 MTR_8g468210 66.667 96 32 0 87 182 77 172 8.31e-37 134
MS.gene99021.t1 MTR_5g039000 60.169 118 46 1 66 182 55 172 1.48e-36 133
MS.gene99021.t1 MTR_7g010020 58.559 111 44 1 74 182 65 175 4.33e-32 121
MS.gene99021.t1 MTR_1g061660 53.571 112 41 2 82 193 54 154 8.98e-27 105
MS.gene99021.t1 MTR_7g103340 55.670 97 36 1 87 183 63 152 1.20e-26 105
MS.gene99021.t1 MTR_3g092150 54.945 91 41 0 89 179 40 130 1.04e-25 103
MS.gene99021.t1 MTR_8g026960 47.573 103 53 1 91 193 38 139 1.62e-24 100
MS.gene99021.t1 MTR_5g019680 60.563 71 28 0 87 157 53 123 1.03e-19 86.3
MS.gene99021.t1 MTR_4g100550 46.281 121 52 4 67 185 103 212 3.23e-19 86.7
MS.gene99021.t1 MTR_5g019650 60.563 71 28 0 87 157 53 123 3.94e-19 85.1
MS.gene99021.t1 MTR_5g013010 40.645 155 71 5 37 185 89 228 1.27e-18 85.1
MS.gene99021.t1 MTR_5g014890 45.794 107 49 2 75 181 113 210 1.45e-17 81.6
MS.gene99021.t1 MTR_4g126900 46.154 91 42 1 90 180 91 174 1.91e-17 80.5
MS.gene99021.t1 MTR_8g006705 48.515 101 44 2 87 186 146 239 3.14e-17 81.3
MS.gene99021.t1 MTR_2g061030 44.340 106 52 1 75 180 188 286 4.13e-17 81.3
MS.gene99021.t1 MTR_7g093430 47.872 94 42 1 87 180 160 246 5.31e-17 80.5
MS.gene99021.t1 MTR_1g054285 46.809 94 43 1 87 180 154 240 3.31e-16 78.2
MS.gene99021.t1 MTR_4g097600 43.925 107 52 2 74 180 115 213 1.06e-15 76.3
MS.gene99021.t1 MTR_1g017090 46.316 95 44 1 87 181 130 217 1.75e-15 75.9
MS.gene99021.t1 MTR_4g084750 37.879 132 61 3 87 217 65 176 2.31e-15 73.9
MS.gene99021.t1 MTR_5g013010 41.667 108 50 3 37 139 89 188 6.51e-14 70.1
MS.gene99021.t1 MTR_3g103590 48.611 72 37 0 87 158 145 216 4.67e-13 69.3
MS.gene99021.t1 MTR_2g038625 41.489 94 48 1 87 180 82 168 2.66e-12 65.5
MS.gene99021.t1 MTR_2g038580 41.489 94 48 1 87 180 82 168 2.66e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99021.t1 AT3G01470 47.396 192 74 7 87 273 68 237 3.90e-42 147
MS.gene99021.t1 AT4G40060 54.615 130 53 2 76 202 45 171 5.34e-41 145
MS.gene99021.t1 AT2G22430 68.041 97 31 0 87 183 62 158 5.31e-39 140
MS.gene99021.t1 AT5G65310 68.041 97 31 0 87 183 54 150 1.98e-38 138
MS.gene99021.t1 AT5G65310 68.041 97 31 0 87 183 72 168 2.54e-38 139
MS.gene99021.t1 AT1G69780 64.545 110 38 1 74 182 71 180 2.74e-38 138
MS.gene99021.t1 AT3G01220 41.071 224 113 6 50 261 46 262 3.42e-37 135
MS.gene99021.t1 AT5G15150 60.000 115 44 1 66 180 96 208 2.09e-36 134
MS.gene99021.t1 AT1G26960 59.091 110 44 1 74 182 57 166 1.27e-33 124
MS.gene99021.t1 AT1G27045 46.939 147 60 3 37 183 36 164 1.05e-32 121
MS.gene99021.t1 AT1G27045 60.825 97 38 0 87 183 25 121 9.87e-32 117
MS.gene99021.t1 AT1G27045 60.825 97 38 0 87 183 25 121 9.87e-32 117
MS.gene99021.t1 AT1G27045 60.825 97 38 0 87 183 17 113 1.45e-31 117
MS.gene99021.t1 AT1G27045 46.259 147 60 4 37 183 36 163 1.93e-30 116
MS.gene99021.t1 AT2G46680 49.541 109 55 0 83 191 28 136 1.33e-28 111
MS.gene99021.t1 AT3G61890 51.429 105 50 1 90 194 33 136 1.67e-27 108
MS.gene99021.t1 AT2G46680 53.763 93 43 0 83 175 28 120 8.24e-27 106
MS.gene99021.t1 AT5G03790 53.608 97 38 1 87 183 77 166 1.79e-25 102
MS.gene99021.t1 AT5G03790 54.839 93 35 1 87 179 77 162 2.25e-24 98.6
MS.gene99021.t1 AT2G36610 47.222 108 42 2 84 183 68 168 8.17e-24 96.7
MS.gene99021.t1 AT4G36740 48.182 110 50 1 87 196 56 158 1.36e-22 94.4
MS.gene99021.t1 AT4G36740 48.182 110 50 1 87 196 55 157 1.49e-22 94.4
MS.gene99021.t1 AT2G18550 45.378 119 58 1 87 205 61 172 2.06e-22 94.0
MS.gene99021.t1 AT5G53980 46.392 97 52 0 87 183 11 107 6.85e-21 88.2
MS.gene99021.t1 AT5G66700 58.333 72 30 0 87 158 71 142 4.19e-20 87.8
MS.gene99021.t1 AT5G06710 48.421 95 42 1 87 181 190 277 3.57e-17 81.6
MS.gene99021.t1 AT4G17460 45.217 115 49 4 75 186 125 228 1.07e-16 79.3
MS.gene99021.t1 AT2G01430 39.706 136 64 4 50 180 103 225 1.68e-16 79.0
MS.gene99021.t1 AT2G44910 47.000 100 45 2 87 185 163 255 4.21e-16 78.2
MS.gene99021.t1 AT3G60390 47.000 100 45 2 87 185 162 254 6.42e-16 77.8
MS.gene99021.t1 AT5G47370 38.931 131 65 3 57 186 107 223 7.38e-16 77.0
MS.gene99021.t1 AT2G22800 45.263 95 45 1 87 181 113 200 9.53e-16 76.6
MS.gene99021.t1 AT4G37790 44.681 94 45 1 87 180 126 212 1.77e-15 75.9
MS.gene99021.t1 AT4G16780 46.000 100 46 2 87 185 129 221 2.55e-15 75.5
MS.gene99021.t1 AT5G06710 58.491 53 22 0 87 139 190 242 1.47e-12 67.4
MS.gene99021.t1 AT1G70920 43.299 97 48 1 84 180 66 155 1.53e-12 66.2
MS.gene99021.t1 AT1G70920 43.299 97 48 1 84 180 36 125 2.19e-12 65.1
MS.gene99021.t1 AT2G01430 41.053 95 45 3 50 139 103 191 3.38e-12 65.5

Find 51 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGAAAATTCTTGTAATTT+TGG 0.015177 5.4:-27560896 MS.gene99021:CDS
TCAATTACAAATTCAGAAAA+TGG 0.161025 5.4:-27561187 MS.gene99021:CDS
CTGAAGCCCTTGAATCTCTT+TGG 0.177337 5.4:-27562550 MS.gene99021:CDS
TGAACCTTGCCCTTCTGTTT+TGG 0.204900 5.4:+27561843 None:intergenic
TTCTCTTTCTCTTTATCTCT+TGG 0.249115 5.4:+27561280 None:intergenic
GCAAGTTGAACTTTCCTATC+AGG 0.270065 5.4:+27561908 None:intergenic
TAGCAAACATCTTCAACTTT+TGG 0.273698 5.4:+27560929 None:intergenic
TGAAGCCCTTGAATCTCTTT+GGG 0.300479 5.4:-27562549 MS.gene99021:CDS
TGAGGTGAATTGATTGCTTC+TGG 0.314138 5.4:+27561238 None:intergenic
CAAAGTGTCACTCCCTAATA+TGG 0.317058 5.4:-27561161 MS.gene99021:CDS
AACCAAGGCAAGTTGCTATA+TGG 0.341312 5.4:-27561868 MS.gene99021:CDS
GTGGAAATTTAAATTCAATA+AGG 0.341810 5.4:+27560839 None:intergenic
TCTCTTTCTCTTTATCTCTT+GGG 0.342274 5.4:+27561281 None:intergenic
CAAAAGGTTTGATCTTCAAC+AGG 0.362492 5.4:+27560869 None:intergenic
TTTCTCAAGTTGTTGAAAGA+AGG 0.381022 5.4:+27562054 None:intergenic
GATAGCCCACATTGCAATGA+TGG 0.423802 5.4:-27561082 MS.gene99021:CDS
ATTGGATCTTTGTTGTTATG+AGG 0.430991 5.4:+27561220 None:intergenic
GTAATTGAGATTATATCCAT+TGG 0.466626 5.4:+27561202 None:intergenic
TTCTCAAGTTGTTGAAAGAA+GGG 0.467149 5.4:+27562055 None:intergenic
CATATTAGGGAGTGACACTT+TGG 0.480614 5.4:+27561162 None:intergenic
GTTCAACTTGCAAAAGAGCT+TGG 0.481261 5.4:-27561896 MS.gene99021:CDS
AACCATATAGCAACTTGCCT+TGG 0.481745 5.4:+27561866 None:intergenic
TGTGAGAAATCTGAATGATC+AGG 0.485138 5.4:+27561007 None:intergenic
CAATTACAAATTCAGAAAAT+GGG 0.485544 5.4:-27561186 MS.gene99021:CDS
GAATGACAAGTTAAAAGAAG+AGG 0.495747 5.4:-27561735 MS.gene99021:intron
TATAAGGCTGTTCTTATGGC+AGG 0.496797 5.4:-27562638 None:intergenic
TCAATAAGGCCAGAAACAAA+AGG 0.504416 5.4:+27560853 None:intergenic
AAAACAAGCTTGAACCTGAT+AGG 0.509669 5.4:-27561922 MS.gene99021:CDS
AAGAGCTTGGTTTGCAACCA+AGG 0.513381 5.4:-27561883 MS.gene99021:CDS
ACCATCAACAAGGAAAGAAA+AGG 0.521051 5.4:-27561994 MS.gene99021:CDS
TGTGAACCCAAAGAGATTCA+AGG 0.525903 5.4:+27562543 None:intergenic
AGATTTCTCACAAGATGAAG+AGG 0.546898 5.4:-27560996 MS.gene99021:CDS
TGTTCCCATCATTGCAATGT+GGG 0.548653 5.4:+27561077 None:intergenic
TCATAACAACAAAGATCCAA+TGG 0.563990 5.4:-27561218 MS.gene99021:CDS
TCAAGGGCTTCAGAAGTACA+AGG 0.569376 5.4:+27562560 None:intergenic
GAATTCAGGTTCAAATTCAG+TGG 0.573234 5.4:-27562125 MS.gene99021:intron
GTGAACCCAAAGAGATTCAA+GGG 0.581702 5.4:+27562544 None:intergenic
CTATATGGTTCCAAAACAGA+AGG 0.583686 5.4:-27561853 MS.gene99021:CDS
ATAGCCCACATTGCAATGAT+GGG 0.583931 5.4:-27561081 MS.gene99021:CDS
CTTGAGAAAGAAGAGAATTG+TGG 0.599550 5.4:-27562040 MS.gene99021:CDS
AAAGCATGTGAAGAATCTGT+AGG 0.619583 5.4:+27561034 None:intergenic
ATCAACAAGGAAAGAAAAGG+AGG 0.621166 5.4:-27561991 MS.gene99021:CDS
ATCACCTTGAAAAGAAGCAG+AGG 0.635119 5.4:+27562520 None:intergenic
TTGTTCCCATCATTGCAATG+TGG 0.645404 5.4:+27561076 None:intergenic
AAGGCTGTTCTTATGGCAGG+TGG 0.647915 5.4:-27562635 None:intergenic
GGTTCCAAAACAGAAGGGCA+AGG 0.651221 5.4:-27561847 MS.gene99021:CDS
TATATGGTTCCAAAACAGAA+GGG 0.680215 5.4:-27561852 MS.gene99021:CDS
AAAAGGTTTGATCTTCAACA+GGG 0.685076 5.4:+27560870 None:intergenic
GAAGCATGTTACCATCAACA+AGG 0.696274 5.4:-27562004 MS.gene99021:CDS
CAAGGGCTTCAGAAGTACAA+GGG 0.720149 5.4:+27562561 None:intergenic
TGGTGGTGGAAGCAACAGAG+TGG 0.722441 5.4:-27562089 MS.gene99021:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTATTTTGTTACAAT+AGG - chr5.4:27562147-27562166 MS.gene99021:intron 10.0%
!!! TAAAAAAGACTATTTTTTTA+TGG + chr5.4:27561243-27561262 None:intergenic 10.0%
!! TGAAAATTTACATTAGAATT+TGG + chr5.4:27561881-27561900 None:intergenic 15.0%
!!! ATGAAAATTCTTGTAATTTT+GGG - chr5.4:27562581-27562600 MS.gene99021:CDS 15.0%
!!! TGAAAAAGACTAATTTTATA+TGG + chr5.4:27561278-27561297 None:intergenic 15.0%
!! AATGAGTTAATGATTGAATA+TGG - chr5.4:27562107-27562126 MS.gene99021:CDS 20.0%
!! ATGAGTTAATGATTGAATAT+GGG - chr5.4:27562108-27562127 MS.gene99021:CDS 20.0%
!! CAAACTTTATTAAAGTAAAG+AGG + chr5.4:27562073-27562092 None:intergenic 20.0%
!! CAATTACAAATTCAGAAAAT+GGG - chr5.4:27562290-27562309 MS.gene99021:intron 20.0%
!! CATATAGTTGAAATATAACA+TGG - chr5.4:27561311-27561330 MS.gene99021:intron 20.0%
!! TCAATTACAAATTCAGAAAA+TGG - chr5.4:27562289-27562308 MS.gene99021:intron 20.0%
!!! AAATTACAAGAATTTTCATG+TGG + chr5.4:27562580-27562599 None:intergenic 20.0%
!!! AATTACAAGAATTTTCATGT+GGG + chr5.4:27562579-27562598 None:intergenic 20.0%
!!! CATGAAAATTCTTGTAATTT+TGG - chr5.4:27562580-27562599 MS.gene99021:CDS 20.0%
!!! TACATTTTAGTACCATATTA+GGG + chr5.4:27562330-27562349 None:intergenic 20.0%
!!! TTACATTTTAGTACCATATT+AGG + chr5.4:27562331-27562350 None:intergenic 20.0%
! AAACAGCTTGAAAAAGATTA+TGG - chr5.4:27561661-27561680 MS.gene99021:intron 25.0%
! CAATTCAAATATACACATGA+TGG + chr5.4:27561052-27561071 None:intergenic 25.0%
! CATGTGTATATTTGAATTGA+CGG - chr5.4:27561053-27561072 MS.gene99021:CDS 25.0%
! GTAATTGAGATTATATCCAT+TGG + chr5.4:27562277-27562296 None:intergenic 25.0%
! TCAATCATTAACTCATTAGT+GGG + chr5.4:27562104-27562123 None:intergenic 25.0%
! TGAGTTAATGATTGAATATG+GGG - chr5.4:27562109-27562128 MS.gene99021:CDS 25.0%
! TTCAATCATTAACTCATTAG+TGG + chr5.4:27562105-27562124 None:intergenic 25.0%
! TTTCTTAAGATCATGAAAGT+GGG - chr5.4:27561194-27561213 MS.gene99021:CDS 25.0%
!! ATTTCTTGAAAAGAGTTTTG+AGG - chr5.4:27561528-27561547 MS.gene99021:intron 25.0%
AAAAGAAGTGAGAAGAATTG+AGG + chr5.4:27560990-27561009 None:intergenic 30.0%
AAAAGGTTTGATCTTCAACA+GGG + chr5.4:27562609-27562628 None:intergenic 30.0%
CAACTGAAAGTTTGTATTGA+CGG + chr5.4:27561114-27561133 None:intergenic 30.0%
GAATGACAAGTTAAAAGAAG+AGG - chr5.4:27561741-27561760 MS.gene99021:CDS 30.0%
GTTTCTTAAGATCATGAAAG+TGG - chr5.4:27561193-27561212 MS.gene99021:CDS 30.0%
TATATGGTTCCAAAACAGAA+GGG - chr5.4:27561624-27561643 MS.gene99021:intron 30.0%
TCATAACAACAAAGATCCAA+TGG - chr5.4:27562258-27562277 MS.gene99021:intron 30.0%
TCTCTTTCTCTTTATCTCTT+GGG + chr5.4:27562198-27562217 None:intergenic 30.0%
TTCTCTTTCTCTTTATCTCT+TGG + chr5.4:27562199-27562218 None:intergenic 30.0%
! TAGCAAACATCTTCAACTTT+TGG + chr5.4:27562550-27562569 None:intergenic 30.0%
! TTCTCAAGTTGTTGAAAGAA+GGG + chr5.4:27561424-27561443 None:intergenic 30.0%
! TTTCTCAAGTTGTTGAAAGA+AGG + chr5.4:27561425-27561444 None:intergenic 30.0%
!! AAGATCAAACCTTTTGTTTC+TGG - chr5.4:27562614-27562633 MS.gene99021:CDS 30.0%
!! ATTGGATCTTTGTTGTTATG+AGG + chr5.4:27562259-27562278 None:intergenic 30.0%
!! TCAGTGGTTAATTTTGAGAA+TGG - chr5.4:27561367-27561386 MS.gene99021:intron 30.0%
!!! ATGTGTATGTGTGTTTTGAT+AGG + chr5.4:27562008-27562027 None:intergenic 30.0%
AAAACAAGCTTGAACCTGAT+AGG - chr5.4:27561554-27561573 MS.gene99021:intron 35.0%
AAAGCATGTGAAGAATCTGT+AGG + chr5.4:27562445-27562464 None:intergenic 35.0%
AATTGTCTGATCTCAATCCA+AGG - chr5.4:27561933-27561952 MS.gene99021:CDS 35.0%
ACCATCAACAAGGAAAGAAA+AGG - chr5.4:27561482-27561501 MS.gene99021:intron 35.0%
AGATTTCTCACAAGATGAAG+AGG - chr5.4:27562480-27562499 MS.gene99021:intron 35.0%
ATCAACAAGGAAAGAAAAGG+AGG - chr5.4:27561485-27561504 MS.gene99021:intron 35.0%
CAAAAGGTTTGATCTTCAAC+AGG + chr5.4:27562610-27562629 None:intergenic 35.0%
CGTCAATACAAACTTTCAGT+TGG - chr5.4:27561112-27561131 MS.gene99021:CDS 35.0%
CTATATGGTTCCAAAACAGA+AGG - chr5.4:27561623-27561642 MS.gene99021:intron 35.0%
CTTGAGAAAGAAGAGAATTG+TGG - chr5.4:27561436-27561455 MS.gene99021:intron 35.0%
GTCAACACATCAGTAAATGT+TGG - chr5.4:27561839-27561858 MS.gene99021:CDS 35.0%
GTTAGTAAATCGATGATCCT+TGG + chr5.4:27561953-27561972 None:intergenic 35.0%
TCAATAAGGCCAGAAACAAA+AGG + chr5.4:27562626-27562645 None:intergenic 35.0%
TGTGAGAAATCTGAATGATC+AGG + chr5.4:27562472-27562491 None:intergenic 35.0%
TTGACACACACAAACGAAAT+TGG - chr5.4:27561780-27561799 MS.gene99021:CDS 35.0%
! GAATTCAGGTTCAAATTCAG+TGG - chr5.4:27561351-27561370 MS.gene99021:intron 35.0%
! GTGTTTGATGACATGAATTC+AGG - chr5.4:27561337-27561356 MS.gene99021:intron 35.0%
! TCCTTTTCTTTCCTTGTTGA+TGG + chr5.4:27561486-27561505 None:intergenic 35.0%
! TCTTCAACTTTTGGTAAGCA+AGG + chr5.4:27562541-27562560 None:intergenic 35.0%
!! GTGGTTAATTTTGAGAATGG+TGG - chr5.4:27561370-27561389 MS.gene99021:intron 35.0%
!! GTTAATTTTGAGAATGGTGG+TGG - chr5.4:27561373-27561392 MS.gene99021:intron 35.0%
AACCAAGGCAAGTTGCTATA+TGG - chr5.4:27561608-27561627 MS.gene99021:intron 40.0%
AACCATATAGCAACTTGCCT+TGG + chr5.4:27561613-27561632 None:intergenic 40.0%
AGAAGTGAGAAGAATTGAGG+AGG + chr5.4:27560987-27561006 None:intergenic 40.0%
ATAGCCCACATTGCAATGAT+GGG - chr5.4:27562395-27562414 MS.gene99021:intron 40.0%
ATCACCTTGAAAAGAAGCAG+AGG + chr5.4:27560959-27560978 None:intergenic 40.0%
CATATTAGGGAGTGACACTT+TGG + chr5.4:27562317-27562336 None:intergenic 40.0%
GAAGCATGTTACCATCAACA+AGG - chr5.4:27561472-27561491 MS.gene99021:intron 40.0%
GTGAACCCAAAGAGATTCAA+GGG + chr5.4:27560935-27560954 None:intergenic 40.0%
GTTCAACTTGCAAAAGAGCT+TGG - chr5.4:27561580-27561599 MS.gene99021:intron 40.0%
TGAAGCCCTTGAATCTCTTT+GGG - chr5.4:27560927-27560946 MS.gene99021:CDS 40.0%
TGAGGTGAATTGATTGCTTC+TGG + chr5.4:27562241-27562260 None:intergenic 40.0%
TGTGAACCCAAAGAGATTCA+AGG + chr5.4:27560936-27560955 None:intergenic 40.0%
TGTTCCCATCATTGCAATGT+GGG + chr5.4:27562402-27562421 None:intergenic 40.0%
TTGTTCCCATCATTGCAATG+TGG + chr5.4:27562403-27562422 None:intergenic 40.0%
! CAAAGTGTCACTCCCTAATA+TGG - chr5.4:27562315-27562334 MS.gene99021:intron 40.0%
! GCAAGTTGAACTTTCCTATC+AGG + chr5.4:27561571-27561590 None:intergenic 40.0%
!! CACATCACTTTTAGCTGAGT+TGG + chr5.4:27562365-27562384 None:intergenic 40.0%
CTGAAGCCCTTGAATCTCTT+TGG - chr5.4:27560926-27560945 MS.gene99021:CDS 45.0%
GATAGCCCACATTGCAATGA+TGG - chr5.4:27562394-27562413 MS.gene99021:intron 45.0%
TTTGATAGGTGACCCGTGTT+CGG + chr5.4:27561994-27562013 None:intergenic 45.0%
! CAAGGGCTTCAGAAGTACAA+GGG + chr5.4:27560918-27560937 None:intergenic 45.0%
! CACACCTCTGCTTCTTTTCA+AGG - chr5.4:27560952-27560971 MS.gene99021:CDS 45.0%
! TCAAGGGCTTCAGAAGTACA+AGG + chr5.4:27560919-27560938 None:intergenic 45.0%
! TGAACCTTGCCCTTCTGTTT+TGG + chr5.4:27561636-27561655 None:intergenic 45.0%
!! AAGAGCTTGGTTTGCAACCA+AGG - chr5.4:27561593-27561612 MS.gene99021:intron 45.0%
GGTTCCAAAACAGAAGGGCA+AGG - chr5.4:27561629-27561648 MS.gene99021:intron 50.0%
! ATGGCAGGTGGCAAGCTTTT+TGG - chr5.4:27560853-27560872 MS.gene99021:CDS 50.0%
CATTCCGAGAGTCCGAACAC+GGG - chr5.4:27561979-27561998 MS.gene99021:CDS 55.0%
GCATTCCGAGAGTCCGAACA+CGG - chr5.4:27561978-27561997 MS.gene99021:CDS 55.0%
! GCAGGTGGCAAGCTTTTTGG+TGG - chr5.4:27560856-27560875 MS.gene99021:CDS 55.0%
!! TGGTGGTGGAAGCAACAGAG+TGG - chr5.4:27561387-27561406 MS.gene99021:intron 55.0%
GTGACCCGTGTTCGGACTCT+CGG + chr5.4:27561986-27562005 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 27560853 27562645 27560853 ID=MS.gene99021
chr5.4 mRNA 27560853 27562645 27560853 ID=MS.gene99021.t1;Parent=MS.gene99021
chr5.4 exon 27562525 27562645 27562525 ID=MS.gene99021.t1.exon1;Parent=MS.gene99021.t1
chr5.4 CDS 27562525 27562645 27562525 ID=cds.MS.gene99021.t1;Parent=MS.gene99021.t1
chr5.4 exon 27561736 27562139 27561736 ID=MS.gene99021.t1.exon2;Parent=MS.gene99021.t1
chr5.4 CDS 27561736 27562139 27561736 ID=cds.MS.gene99021.t1;Parent=MS.gene99021.t1
chr5.4 exon 27560853 27561329 27560853 ID=MS.gene99021.t1.exon3;Parent=MS.gene99021.t1
chr5.4 CDS 27560853 27561329 27560853 ID=cds.MS.gene99021.t1;Parent=MS.gene99021.t1
Gene Sequence

>MS.gene99021

ATGGCAGGTGGCAAGCTTTTTGGTGGTTCTAATATGTCACTTTTGCTTCAAAATGAAAGACTCCCTTGTACTTCTGAAGCCCTTGAATCTCTTTGGGTTCACACCTCTGCTTCTTTTCAAGGTGATCTTCACCTCCTCAATTCTTCTCACTTCTTTTGTTACATAATTGTCATCAGTTTTTGTAGTTGTTTCTTCACCATCATGTGTATATTTGAATTGACGGTGAGTTAGACAGAATCACTGATTCTGTCTAACTTACCGTCAATACAAACTTTCAGTTGGTCTTAAAATTTCACAATTTTAAGTTTTATGAATATCATAGAGCATAACACACACATATGTTTCTTAAGATCATGAAAGTGGGTTTCTTTTTTCATTGTGAAGAAACCATAAAAAAATAGTCTTTTTTATTTTTGAAGAAACCATATAAAATTAGTCTTTTTCATTATTTTTCTATGCATATAGTTGAAATATAACATGGTTTGTGTTTGATGACATGAATTCAGGTTCAAATTCAGTGGTTAATTTTGAGAATGGTGGTGGAAGCAACAGAGTGGTAACAGATAAACCCTTCTTTCAACAACTTGAGAAAGAAGAGAATTGTGGTGATGAAGATTATGAAGCATGTTACCATCAACAAGGAAAGAAAAGGAGGCTTTCAAGTGAACAAGTTCAATTTCTTGAAAAGAGTTTTGAGGTAGAAAACAAGCTTGAACCTGATAGGAAAGTTCAACTTGCAAAAGAGCTTGGTTTGCAACCAAGGCAAGTTGCTATATGGTTCCAAAACAGAAGGGCAAGGTTCAAAACTAAACAGCTTGAAAAAGATTATGGCACATTGAAAGCTAGCTTTGATAGTCTCAAAGATGATTATGATAATCTTCTTCAAGAGAATGACAAGTTAAAAGAAGAGGTAAAAAAAGTAAAAAGTTGACACACACAAACGAAATTGGACACTATGTTGTCATTGATCGCACGCATTTCACATAGTCAACACATCAGTAAATGTTGGATCGAGATCAGACAATCCAAATTCTAATGTAAATTTTCATCTTAAATCTAAAGTATCAAACTTTAAATTTGAATTGTCTGATCTCAATCCAAGGATCATCGATTTACTAACAGCGTGCATTCCGAGAGTCCGAACACGGGTCACCTATCAAAACACACATACACATAAAAACATACTAATTCAGCTTTTCTACTTTATGCTGCTATGACCTCTTTACTTTAATAAAGTTTGATATTTCTCCCACTAATGAGTTAATGATTGAATATGGGGTTAATTTTATTTTTTATTTTTTATTTTGTTACAATAGGTTAATTCTCTCAAGAACAAATTGATCCCAAGAGATAAAGAGAAAGAGAATTCAGATGAAAAATCATCACCAGAAGCAATCAATTCACCTCATAACAACAAAGATCCAATGGATATAATCTCAATTACAAATTCAGAAAATGGGTCCAAAGTGTCACTCCCTAATATGGTACTAAAATGTAAGCAAGAAGATGCCAACTCAGCTAAAAGTGATGTGCTTGATTCTGATAGCCCACATTGCAATGATGGGAACAATCTTTCTTCTTTCATAGAGCCTACAGATTCTTCACATGCTTTTGAACCTGATCATTCAGATTTCTCACAAGATGAAGAGGATAATGATAACTTGAGTCATAATCTTTTGACTCTTCCTTGCTTACCAAAAGTTGAAGATGTTTGCTATGATGACCCACATGAAAATTCTTGTAATTTTGGGTTCCCTGTTGAAGATCAAACCTTTTGTTTCTGGCCTTATTGA
Protein sequence

>MS.gene99021.t1

MAGGKLFGGSNMSLLLQNERLPCTSEALESLWVHTSASFQGSNSVVNFENGGGSNRVVTDKPFFQQLEKEENCGDEDYEACYHQQGKKRRLSSEQVQFLEKSFEVENKLEPDRKVQLAKELGLQPRQVAIWFQNRRARFKTKQLEKDYGTLKASFDSLKDDYDNLLQENDKLKEEVNSLKNKLIPRDKEKENSDEKSSPEAINSPHNNKDPMDIISITNSENGSKVSLPNMVLKCKQEDANSAKSDVLDSDSPHCNDGNNLSSFIEPTDSSHAFEPDHSDFSQDEEDNDNLSHNLLTLPCLPKVEDVCYDDPHENSCNFGFPVEDQTFCFWPY