Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99292.t1 | XP_013467291.1 | 88.3 | 247 | 29 | 0 | 1 | 247 | 1 | 247 | 1.70E-114 | 422.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99292.t1 | Q9M1K1 | 41.0 | 251 | 133 | 5 | 1 | 240 | 1 | 247 | 2.4e-40 | 167.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99292.t1 | A0A072VIS7 | 88.3 | 247 | 29 | 0 | 1 | 247 | 1 | 247 | 1.2e-114 | 422.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene99292.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99292.t1 | MTR_1g048720 | 88.259 | 247 | 29 | 0 | 1 | 247 | 1 | 247 | 3.39e-161 | 446 |
| MS.gene99292.t1 | MTR_1g048720 | 87.854 | 247 | 29 | 1 | 1 | 247 | 1 | 246 | 1.13e-158 | 440 |
| MS.gene99292.t1 | MTR_1g048720 | 88.584 | 219 | 25 | 0 | 1 | 219 | 1 | 219 | 2.56e-144 | 402 |
| MS.gene99292.t1 | MTR_1g048720 | 88.128 | 219 | 25 | 1 | 1 | 219 | 1 | 218 | 7.65e-142 | 396 |
| MS.gene99292.t1 | MTR_1g048710 | 84.836 | 244 | 28 | 2 | 1 | 244 | 1 | 235 | 1.79e-141 | 396 |
| MS.gene99292.t1 | MTR_1g048750 | 88.532 | 218 | 17 | 1 | 1 | 218 | 1 | 210 | 5.31e-139 | 391 |
| MS.gene99292.t1 | MTR_7g090410 | 58.964 | 251 | 84 | 7 | 1 | 241 | 1 | 242 | 4.34e-67 | 208 |
| MS.gene99292.t1 | MTR_0007s0330 | 32.075 | 159 | 96 | 6 | 65 | 220 | 6 | 155 | 7.54e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99292.t1 | AT3G56970 | 41.434 | 251 | 132 | 6 | 1 | 240 | 1 | 247 | 9.68e-55 | 176 |
| MS.gene99292.t1 | AT3G56980 | 39.927 | 273 | 114 | 6 | 1 | 242 | 1 | 254 | 2.71e-46 | 155 |
| MS.gene99292.t1 | AT2G41240 | 38.057 | 247 | 139 | 4 | 1 | 242 | 1 | 238 | 1.95e-44 | 150 |
| MS.gene99292.t1 | AT2G41240 | 37.652 | 247 | 139 | 5 | 1 | 242 | 1 | 237 | 5.97e-42 | 143 |
| MS.gene99292.t1 | AT5G04150 | 46.012 | 163 | 83 | 2 | 60 | 219 | 78 | 238 | 4.19e-36 | 128 |
| MS.gene99292.t1 | AT5G04150 | 44.262 | 183 | 94 | 4 | 60 | 237 | 78 | 257 | 5.86e-36 | 128 |
Find 37 sgRNAs with CRISPR-Local
Find 51 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCACATGTTTCTGTATTTC+TGG | 0.048296 | 1.3:+30988087 | None:intergenic |
| TGATGCTGGTTATGGCTTAT+TGG | 0.196697 | 1.3:+30988508 | None:intergenic |
| CGAGAAGTTGTTATCAATAA+TGG | 0.272808 | 1.3:-30987642 | MS.gene99292:CDS |
| TTCTGAGATCTTGATGTGTT+TGG | 0.297904 | 1.3:-30987875 | MS.gene99292:CDS |
| GTTTCTTCATCTCAAACCTT+TGG | 0.309294 | 1.3:-30987826 | MS.gene99292:CDS |
| ATGGATTCTGATGCTGGTTA+TGG | 0.357369 | 1.3:+30988500 | None:intergenic |
| AGTACTCTTGAAATTGTTGC+TGG | 0.363324 | 1.3:+30988120 | None:intergenic |
| TCAATAGTGTCTTTGTAGAA+TGG | 0.399191 | 1.3:+30988481 | None:intergenic |
| ATGGCTTATTGGCTCTTCAA+AGG | 0.435523 | 1.3:+30988519 | None:intergenic |
| TCTTCCTGTTTCAGATCAAA+TGG | 0.439923 | 1.3:-30988293 | MS.gene99292:intron |
| AGTACCATTTGATCTGAAAC+AGG | 0.450182 | 1.3:+30988289 | None:intergenic |
| TCTTCATCTCAAACCTTTGG+AGG | 0.450222 | 1.3:-30987823 | MS.gene99292:CDS |
| TGTAGAATGGATTCTGATGC+TGG | 0.456368 | 1.3:+30988494 | None:intergenic |
| GTTGTTATCAATAATGGAAA+AGG | 0.460271 | 1.3:-30987636 | MS.gene99292:CDS |
| ATCTCAAACCTTTGGAGGGA+GGG | 0.471487 | 1.3:-30987818 | MS.gene99292:CDS |
| AAGAAACATTTAGCAACAAA+AGG | 0.474589 | 1.3:+30987842 | None:intergenic |
| CACATCAAGATCTCAGAAAG+TGG | 0.484201 | 1.3:+30987880 | None:intergenic |
| TGGCTTATTGGCTCTTCAAA+GGG | 0.488560 | 1.3:+30988520 | None:intergenic |
| ACAAATTATTCATCTTCTTG+CGG | 0.523820 | 1.3:+30988335 | None:intergenic |
| CTTCATCTCAAACCTTTGGA+GGG | 0.524238 | 1.3:-30987822 | MS.gene99292:CDS |
| ATAATAACTTCATTTACTTG+TGG | 0.527287 | 1.3:+30988021 | None:intergenic |
| AAGAAACATAGTTACAACTC+TGG | 0.547575 | 1.3:-30987979 | MS.gene99292:CDS |
| CATCTCAAACCTTTGGAGGG+AGG | 0.551061 | 1.3:-30987819 | MS.gene99292:CDS |
| CATGCCAAGGTCACTGCTTG+TGG | 0.551876 | 1.3:+30988396 | None:intergenic |
| CATCCAATTGTTGAAAACAT+AGG | 0.558093 | 1.3:+30988544 | None:intergenic |
| CAATCCACAAGCAGTGACCT+TGG | 0.569748 | 1.3:-30988400 | MS.gene99292:CDS |
| GTAGAAGACCCTCCCTCCAA+AGG | 0.574235 | 1.3:+30987810 | None:intergenic |
| GTCACTGCTTGTGGATTGTG+AGG | 0.591686 | 1.3:+30988405 | None:intergenic |
| CCAATTGTTGAAAACATAGG+AGG | 0.594118 | 1.3:+30988547 | None:intergenic |
| TTGATGTGTTTGGAAAATGA+TGG | 0.597620 | 1.3:-30987865 | MS.gene99292:CDS |
| CACAAGCAGTGACCTTGGCA+TGG | 0.603384 | 1.3:-30988395 | MS.gene99292:CDS |
| TTATCAATAATGGAAAAGGA+AGG | 0.604075 | 1.3:-30987632 | MS.gene99292:CDS |
| GAAATACAGAAACATGTGGA+AGG | 0.627895 | 1.3:-30988084 | MS.gene99292:CDS |
| CTGAAACAGGAAGAAGTGAA+CGG | 0.644676 | 1.3:+30988302 | None:intergenic |
| ACCAGAAATACAGAAACATG+TGG | 0.649518 | 1.3:-30988088 | MS.gene99292:CDS |
| CAAGCTTCTTGACCATGCCA+AGG | 0.656633 | 1.3:+30988383 | None:intergenic |
| TCACTGCTTGTGGATTGTGA+GGG | 0.698792 | 1.3:+30988406 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTGTAAATAGTAAATTC+AGG | - | chr1.3:30988434-30988453 | MS.gene99292:CDS | 20.0% |
| !! | ATAATAACTTCATTTACTTG+TGG | + | chr1.3:30988157-30988176 | None:intergenic | 20.0% |
| !!! | TTTCTTCTTTTTAGATTCTT+TGG | + | chr1.3:30988182-30988201 | None:intergenic | 20.0% |
| !!! | TTTTGATTTTCTTAGAACTA+TGG | - | chr1.3:30987981-30988000 | MS.gene99292:CDS | 20.0% |
| ! | AAGAAACATTTAGCAACAAA+AGG | + | chr1.3:30988336-30988355 | None:intergenic | 25.0% |
| ! | ACAAATTATTCATCTTCTTG+CGG | + | chr1.3:30987843-30987862 | None:intergenic | 25.0% |
| ! | CAACTTCTCGTTTAAAAAAT+CGG | + | chr1.3:30988523-30988542 | None:intergenic | 25.0% |
| !! | ATTTAACTAAAAGCTCCTTT+TGG | - | chr1.3:30988461-30988480 | MS.gene99292:CDS | 25.0% |
| !! | GTTGTTATCAATAATGGAAA+AGG | - | chr1.3:30988539-30988558 | MS.gene99292:CDS | 25.0% |
| !! | TTATCAATAATGGAAAAGGA+AGG | - | chr1.3:30988543-30988562 | MS.gene99292:CDS | 25.0% |
| !! | TTTAACTAAAAGCTCCTTTT+GGG | - | chr1.3:30988462-30988481 | MS.gene99292:CDS | 25.0% |
| AAGAAACATAGTTACAACTC+TGG | - | chr1.3:30988196-30988215 | MS.gene99292:intron | 30.0% | |
| CATCCAATTGTTGAAAACAT+AGG | + | chr1.3:30987634-30987653 | None:intergenic | 30.0% | |
| CGAGAAGTTGTTATCAATAA+TGG | - | chr1.3:30988533-30988552 | MS.gene99292:CDS | 30.0% | |
| TCAATAGTGTCTTTGTAGAA+TGG | + | chr1.3:30987697-30987716 | None:intergenic | 30.0% | |
| TTGATGTGTTTGGAAAATGA+TGG | - | chr1.3:30988310-30988329 | MS.gene99292:CDS | 30.0% | |
| ! | CTATGTTTTCAACAATTGGA+TGG | - | chr1.3:30987632-30987651 | MS.gene99292:CDS | 30.0% |
| ! | CTTCTACAATTTGCATTTTC+AGG | - | chr1.3:30988381-30988400 | MS.gene99292:CDS | 30.0% |
| ! | GTTTTGTAGTTTCAACAAGT+AGG | - | chr1.3:30988218-30988237 | MS.gene99292:intron | 30.0% |
| !! | TCATTTTGATGATCAAGTAG+AGG | - | chr1.3:30987726-30987745 | MS.gene99292:intron | 30.0% |
| ACCAGAAATACAGAAACATG+TGG | - | chr1.3:30988087-30988106 | MS.gene99292:CDS | 35.0% | |
| AGTACTCTTGAAATTGTTGC+TGG | + | chr1.3:30988058-30988077 | None:intergenic | 35.0% | |
| CCAATTGTTGAAAACATAGG+AGG | + | chr1.3:30987631-30987650 | None:intergenic | 35.0% | |
| GAAATACAGAAACATGTGGA+AGG | - | chr1.3:30988091-30988110 | MS.gene99292:CDS | 35.0% | |
| GTTTCTTCATCTCAAACCTT+TGG | - | chr1.3:30988349-30988368 | MS.gene99292:CDS | 35.0% | |
| TCTTCCTGTTTCAGATCAAA+TGG | - | chr1.3:30987882-30987901 | MS.gene99292:CDS | 35.0% | |
| ! | AGTACCATTTGATCTGAAAC+AGG | + | chr1.3:30987889-30987908 | None:intergenic | 35.0% |
| ! | CCTCCTATGTTTTCAACAAT+TGG | - | chr1.3:30987628-30987647 | MS.gene99292:CDS | 35.0% |
| ! | TCCACATGTTTCTGTATTTC+TGG | + | chr1.3:30988091-30988110 | None:intergenic | 35.0% |
| !! | TTCTGAGATCTTGATGTGTT+TGG | - | chr1.3:30988300-30988319 | MS.gene99292:CDS | 35.0% |
| CACATCAAGATCTCAGAAAG+TGG | + | chr1.3:30988298-30988317 | None:intergenic | 40.0% | |
| CTGAAACAGGAAGAAGTGAA+CGG | + | chr1.3:30987876-30987895 | None:intergenic | 40.0% | |
| CTTCATCTCAAACCTTTGGA+GGG | - | chr1.3:30988353-30988372 | MS.gene99292:CDS | 40.0% | |
| TCTTCATCTCAAACCTTTGG+AGG | - | chr1.3:30988352-30988371 | MS.gene99292:CDS | 40.0% | |
| ! | ATGGATTCTGATGCTGGTTA+TGG | + | chr1.3:30987678-30987697 | None:intergenic | 40.0% |
| ! | TGATGCTGGTTATGGCTTAT+TGG | + | chr1.3:30987670-30987689 | None:intergenic | 40.0% |
| ! | TGTAGAATGGATTCTGATGC+TGG | + | chr1.3:30987684-30987703 | None:intergenic | 40.0% |
| !! | ATGGCTTATTGGCTCTTCAA+AGG | + | chr1.3:30987659-30987678 | None:intergenic | 40.0% |
| !! | TGGCTTATTGGCTCTTCAAA+GGG | + | chr1.3:30987658-30987677 | None:intergenic | 40.0% |
| ! | ATCTCAAACCTTTGGAGGGA+GGG | - | chr1.3:30988357-30988376 | MS.gene99292:CDS | 45.0% |
| ! | TCACTGCTTGTGGATTGTGA+GGG | + | chr1.3:30987772-30987791 | None:intergenic | 45.0% |
| CAAGCTTCTTGACCATGCCA+AGG | + | chr1.3:30987795-30987814 | None:intergenic | 50.0% | |
| CAATCCACAAGCAGTGACCT+TGG | - | chr1.3:30987775-30987794 | MS.gene99292:intron | 50.0% | |
| CCACCTGCAGAAAACCCAAA+AGG | + | chr1.3:30988479-30988498 | None:intergenic | 50.0% | |
| ! | CATCTCAAACCTTTGGAGGG+AGG | - | chr1.3:30988356-30988375 | MS.gene99292:CDS | 50.0% |
| ! | GTCACTGCTTGTGGATTGTG+AGG | + | chr1.3:30987773-30987792 | None:intergenic | 50.0% |
| !!! | CCTTTTGGGTTTTCTGCAGG+TGG | - | chr1.3:30988476-30988495 | MS.gene99292:CDS | 50.0% |
| !!! | GCTCCTTTTGGGTTTTCTGC+AGG | - | chr1.3:30988473-30988492 | MS.gene99292:CDS | 50.0% |
| CACAAGCAGTGACCTTGGCA+TGG | - | chr1.3:30987780-30987799 | MS.gene99292:intron | 55.0% | |
| CATGCCAAGGTCACTGCTTG+TGG | + | chr1.3:30987782-30987801 | None:intergenic | 55.0% | |
| GTAGAAGACCCTCCCTCCAA+AGG | + | chr1.3:30988368-30988387 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 30987613 | 30988584 | 30987613 | ID=MS.gene99292 |
| chr1.3 | mRNA | 30987613 | 30988584 | 30987613 | ID=MS.gene99292.t1;Parent=MS.gene99292 |
| chr1.3 | exon | 30988294 | 30988584 | 30988294 | ID=MS.gene99292.t1.exon1;Parent=MS.gene99292.t1 |
| chr1.3 | CDS | 30988294 | 30988584 | 30988294 | ID=cds.MS.gene99292.t1;Parent=MS.gene99292.t1 |
| chr1.3 | exon | 30987795 | 30988157 | 30987795 | ID=MS.gene99292.t1.exon2;Parent=MS.gene99292.t1 |
| chr1.3 | CDS | 30987795 | 30988157 | 30987795 | ID=cds.MS.gene99292.t1;Parent=MS.gene99292.t1 |
| chr1.3 | exon | 30987613 | 30987702 | 30987613 | ID=MS.gene99292.t1.exon3;Parent=MS.gene99292.t1 |
| chr1.3 | CDS | 30987613 | 30987702 | 30987613 | ID=cds.MS.gene99292.t1;Parent=MS.gene99292.t1 |
| Gene Sequence |
| Protein sequence |
