AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99367

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99367.t1 MTR_5g088060 86.117 461 47 2 12 459 5 461 0.0 777
MS.gene99367.t1 MTR_5g488160 86.117 461 47 2 12 459 5 461 0.0 777
MS.gene99367.t1 MTR_5g088010 88.933 253 19 3 212 459 1 249 1.58e-150 428
MS.gene99367.t1 MTR_5g088005 94.410 161 9 0 1 161 1 161 3.64e-106 312
MS.gene99367.t1 MTR_5g088200 65.984 244 19 4 1 180 1 244 8.59e-92 298
MS.gene99367.t1 MTR_5g027570 66.383 235 64 7 183 406 1 231 2.93e-86 264
MS.gene99367.t1 MTR_5g027550 48.973 292 71 8 183 459 1 229 9.85e-64 206
MS.gene99367.t1 MTR_5g027770 56.329 158 36 5 6 130 153 310 7.42e-38 140
MS.gene99367.t1 MTR_5g027770 45.517 145 27 5 1 125 1 113 1.03e-18 87.0
MS.gene99367.t1 MTR_5g027770 56.098 82 22 3 196 271 98 171 1.25e-14 75.1
MS.gene99367.t1 MTR_5g027780 55.660 106 25 2 20 117 36 127 1.84e-25 103
MS.gene99367.t1 MTR_8g063600 44.167 120 64 3 224 341 62 180 2.14e-22 97.4
MS.gene99367.t1 MTR_5g069710 58.228 79 29 2 268 345 134 209 2.38e-22 97.4
MS.gene99367.t1 MTR_8g063870 51.064 94 45 1 248 341 74 166 4.26e-22 95.9
MS.gene99367.t1 MTR_3g462790 56.627 83 32 2 268 349 149 228 5.29e-22 96.7
MS.gene99367.t1 MTR_1g111830 46.364 110 43 3 268 376 126 220 2.54e-21 93.2
MS.gene99367.t1 MTR_7g067080 46.087 115 48 3 268 380 142 244 2.84e-21 94.4
MS.gene99367.t1 MTR_5g081860 56.790 81 33 2 255 334 113 192 3.12e-21 93.6
MS.gene99367.t1 MTR_4g111975 53.165 79 32 3 264 340 135 210 5.71e-20 90.5
MS.gene99367.t1 MTR_1g048660 55.556 72 29 1 268 339 14 82 1.11e-18 81.3
MS.gene99367.t1 MTR_3g104370 48.571 70 36 0 268 337 93 162 2.51e-18 84.3
MS.gene99367.t1 MTR_5g075760 41.379 116 65 2 223 338 64 176 5.78e-18 82.8
MS.gene99367.t1 MTR_2g100930 53.846 65 27 1 268 332 111 172 9.51e-18 84.7
MS.gene99367.t1 MTR_4g100630 39.831 118 54 5 226 332 47 158 7.80e-17 81.6
MS.gene99367.t1 MTR_6g092540 52.308 65 28 1 268 332 99 160 5.10e-16 78.6
MS.gene99367.t1 MTR_8g101650 65.217 46 16 0 268 313 107 152 5.16e-16 78.6
MS.gene99367.t1 MTR_5g037080 67.391 46 15 0 268 313 104 149 6.76e-16 79.0
MS.gene99367.t1 MTR_4g107230 68.889 45 14 0 268 312 86 130 7.51e-16 78.2
MS.gene99367.t1 MTR_5g075790 38.889 108 56 2 240 338 78 184 1.72e-15 75.9
MS.gene99367.t1 MTR_4g015130 56.667 60 23 1 276 335 3 59 1.82e-15 72.0
MS.gene99367.t1 MTR_2g090305 41.026 78 43 1 268 345 30 104 8.01e-15 73.2
MS.gene99367.t1 MTR_0036s0260 39.744 78 44 1 268 345 30 104 9.67e-15 73.2
MS.gene99367.t1 MTR_1g083180 38.889 126 56 7 234 341 13 135 1.67e-14 72.0
MS.gene99367.t1 MTR_1g033620 55.932 59 22 2 282 339 21 76 8.78e-14 71.2
MS.gene99367.t1 MTR_3g070110 46.667 90 40 3 267 348 96 185 1.37e-13 70.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99367.t1 AT5G58900 41.176 136 64 3 268 398 143 267 4.79e-23 99.0
MS.gene99367.t1 AT5G08520 56.790 81 31 2 264 343 115 192 1.39e-22 98.2
MS.gene99367.t1 AT5G04760 60.526 76 27 1 259 334 92 164 1.55e-22 95.9
MS.gene99367.t1 AT5G01200 61.429 70 23 2 268 336 148 214 3.62e-21 93.2
MS.gene99367.t1 AT2G38090 57.692 78 28 3 261 336 135 209 1.60e-20 92.0
MS.gene99367.t1 AT1G49010 53.659 82 33 3 264 343 131 209 4.14e-20 91.3
MS.gene99367.t1 AT5G61620 43.651 126 51 5 260 377 101 214 5.19e-19 88.2
MS.gene99367.t1 AT3G11280 48.936 94 39 3 268 357 129 217 2.26e-18 85.1
MS.gene99367.t1 AT3G11280 48.936 94 39 3 268 357 129 217 2.26e-18 85.1
MS.gene99367.t1 AT5G23650 52.632 76 33 1 268 343 122 194 2.65e-18 86.3
MS.gene99367.t1 AT3G10580 40.000 130 60 5 255 374 77 198 4.20e-18 84.3
MS.gene99367.t1 AT3G10580 40.000 130 60 5 255 374 77 198 5.66e-18 84.3
MS.gene99367.t1 AT5G05790 55.000 80 28 3 264 338 128 204 1.40e-17 83.2
MS.gene99367.t1 AT5G05790 55.000 80 28 3 264 338 128 204 1.40e-17 83.2
MS.gene99367.t1 AT5G47390 46.591 88 41 3 268 353 98 181 2.23e-17 84.0
MS.gene99367.t1 AT1G70000 67.391 46 15 0 268 313 100 145 1.82e-16 79.7
MS.gene99367.t1 AT1G70000 67.391 46 15 0 268 313 100 145 1.82e-16 79.7
MS.gene99367.t1 AT5G56840 55.172 58 26 0 268 325 93 150 2.16e-16 79.0
MS.gene99367.t1 AT4G09450 53.623 69 29 1 268 336 93 158 3.26e-16 77.4
MS.gene99367.t1 AT3G16350 65.217 46 16 0 268 313 139 184 9.68e-16 79.3
MS.gene99367.t1 AT1G19000 44.681 94 44 3 224 312 58 148 1.06e-15 77.8
MS.gene99367.t1 AT1G19000 44.681 94 44 3 224 312 58 148 1.06e-15 77.8
MS.gene99367.t1 AT1G74840 68.889 45 14 0 268 312 98 142 1.67e-15 76.3
MS.gene99367.t1 AT1G74840 68.889 45 14 0 268 312 98 142 2.32e-15 76.3
MS.gene99367.t1 AT3G10590 50.000 68 30 2 268 335 113 176 1.39e-12 67.0

Find 106 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCCATATCTTTCACATTT+TGG 0.099316 5.1:-69397772 None:intergenic
TCACCTTGTTCTTGGATATT+TGG 0.151843 5.1:-69396463 None:intergenic
TCAACATGATTCATAGGTTT+TGG 0.219569 5.1:-69395772 None:intergenic
CCTGCTGCTGCCGGGGATAC+AGG 0.250754 5.1:-69396337 None:intergenic
CTGTCCAATGCCCTTTCCTT+GGG 0.257949 5.1:-69397399 None:intergenic
GATGTTCCTGTGTTGGTTAT+TGG 0.258752 5.1:+69395712 MS.gene99367:CDS
CTTGGGGCATGACTTTCTTC+TGG 0.281577 5.1:-69397382 None:intergenic
TTCTCAAGTTTGTTCATATC+AGG 0.295923 5.1:-69398157 None:intergenic
TCACATGGTCACCTTGTTCT+TGG 0.300281 5.1:-69396471 None:intergenic
TCTGTCCAATGCCCTTTCCT+TGG 0.314383 5.1:-69397400 None:intergenic
CTGAACATGTTTGGCATCAT+TGG 0.316454 5.1:-69396386 None:intergenic
TGGATATTTGGCCTTCCATT+TGG 0.317326 5.1:-69396451 None:intergenic
ACATTCTCAACATGATTCAT+AGG 0.330941 5.1:-69395778 None:intergenic
CCACCAAAATGTGAAAGATA+TGG 0.352224 5.1:+69397769 MS.gene99367:CDS
TGAGAAGATGCGCAACTTGT+TGG 0.359768 5.1:+69398174 MS.gene99367:CDS
CACCGGAGGCTGAACATGTT+TGG 0.362724 5.1:-69396395 None:intergenic
TTGGATAAAGCAACACCAAC+TGG 0.371101 5.1:-69398120 None:intergenic
AAACAGAAATCAAACGGTTC+TGG 0.380690 5.1:+69396181 MS.gene99367:intron
TTTCTGTTGCGCTTGGGGCC+TGG 0.382290 5.1:-69396250 None:intergenic
TGCTATGCTCAGAGGGATCT+TGG 0.382368 5.1:-69397225 None:intergenic
AACCTTGTCCTGCTGCTGCC+GGG 0.402092 5.1:-69396345 None:intergenic
TTTGTGTTTCTGTTGCGCTT+GGG 0.412088 5.1:-69396256 None:intergenic
AAATATTTCAAAAGAATTTG+TGG 0.425853 5.1:+69397697 MS.gene99367:CDS
TTAGTGCTCCTAAGTCTTCA+AGG 0.427365 5.1:+69395659 MS.gene99367:CDS
ATTTGTGTTTCTGTTGCGCT+TGG 0.430827 5.1:-69396257 None:intergenic
TCCTTTAGTCACTGCTCCTA+TGG 0.430956 5.1:+69398027 MS.gene99367:CDS
GGATTCTCGCCATTAGGATA+AGG 0.435944 5.1:-69396310 None:intergenic
TATGCTGTTAATTATCCAAA+TGG 0.436533 5.1:+69396436 MS.gene99367:CDS
GGCTTAGGATTCTCGCCATT+AGG 0.439906 5.1:-69396316 None:intergenic
TGTGAAAGATATGGAGAAGA+AGG 0.441203 5.1:+69397778 MS.gene99367:CDS
GAACAACAACAGGAGAAAAC+TGG 0.441684 5.1:+69397250 MS.gene99367:CDS
AATCGGTTCTACAATTCCTT+CGG 0.444800 5.1:+69397982 MS.gene99367:CDS
GGTGATGGCATCTTATTCTT+CGG 0.445274 5.1:-69396214 None:intergenic
ATTGTTCTTGTTGTTTAAGA+TGG 0.446908 5.1:-69398083 None:intergenic
AAACCTTGTCCTGCTGCTGC+CGG 0.447814 5.1:-69396346 None:intergenic
ACCACCCGACTCAATGATAT+CGG 0.449690 5.1:-69397275 None:intergenic
ATGTTTGCCCTGGCAGGAAA+AGG 0.462283 5.1:-69396517 None:intergenic
GCGACGATGCCTTATCCTAA+TGG 0.462440 5.1:+69396301 MS.gene99367:CDS
TTGGTGTTGCTTTATCCAAT+CGG 0.462526 5.1:+69398124 MS.gene99367:CDS
CCTGTATCCCCGGCAGCAGC+AGG 0.464792 5.1:+69396337 MS.gene99367:CDS
GGCTTTCGATAGCGCGGTGA+TGG 0.464836 5.1:-69396229 None:intergenic
GCTCATTTAAATCAATTTGA+AGG 0.475975 5.1:-69397192 None:intergenic
TCAAATTGATTTAAATGAGC+CGG 0.477484 5.1:+69397195 MS.gene99367:CDS
TTGTGTTTCTGTTGCGCTTG+GGG 0.482706 5.1:-69396255 None:intergenic
GCATAGCATGGAACAACAAC+AGG 0.485938 5.1:+69397240 MS.gene99367:CDS
GGTCTCTTCCTTGAAGACTT+AGG 0.489770 5.1:-69395667 None:intergenic
TCACCGCGCTATCGAAAGCC+AGG 0.491564 5.1:+69396232 MS.gene99367:CDS
GCTGCCGGGGATACAGGCTT+AGG 0.492601 5.1:-69396331 None:intergenic
AAAGTCATGCCCCAAGGAAA+GGG 0.493395 5.1:+69397389 MS.gene99367:CDS
ATAGGCATGTTTGCCCTGGC+AGG 0.493753 5.1:-69396523 None:intergenic
GAATCCTAAGCCTGTATCCC+CGG 0.504373 5.1:+69396327 MS.gene99367:CDS
TGCCAAACATGTTCAGCCTC+CGG 0.513848 5.1:+69396393 MS.gene99367:CDS
TAATTAACAGCATAAGCATT+CGG 0.516489 5.1:-69396427 None:intergenic
ATGTTGTGCTTGCTGCCAAT+CGG 0.523993 5.1:+69397965 MS.gene99367:CDS
ATGCCCCAAGGAAAGGGCAT+TGG 0.524782 5.1:+69397395 MS.gene99367:CDS
GCATGGCTAGCAATTTGAGT+TGG 0.525594 5.1:-69397731 None:intergenic
AGAAAGAGCTGGCCAAGACT+TGG 0.525727 5.1:-69395737 None:intergenic
GAAAGTCATGCCCCAAGGAA+AGG 0.527463 5.1:+69397388 MS.gene99367:CDS
GTGATTAAACAGAAATCAAA+CGG 0.527832 5.1:+69396175 MS.gene99367:intron
GTCTCATGTTGTTGTTCCAT+AGG 0.529730 5.1:-69398043 None:intergenic
TGGTTATTGGAACCAAGTCT+TGG 0.530265 5.1:+69395725 MS.gene99367:CDS
TTGCATAGGCATGTTTGCCC+TGG 0.530471 5.1:-69396527 None:intergenic
GGCACCGATATCATTGAGTC+GGG 0.531762 5.1:+69397271 MS.gene99367:CDS
CAAGGTGACCATGTGAATCA+TGG 0.533701 5.1:+69396478 MS.gene99367:CDS
TGTTCCATGCTATGCTCAGA+GGG 0.536332 5.1:-69397232 None:intergenic
ATTAGAGTTTAGAATTGCAA+TGG 0.542743 5.1:-69397299 None:intergenic
GTCTGTTGCATTCCTTGAGG+AGG 0.550846 5.1:-69397914 None:intergenic
TTTAAGATGGCAGTGTCTTG+AGG 0.553601 5.1:-69398070 None:intergenic
GGATAAGGCATCGTCGCCAC+AGG 0.553867 5.1:-69396295 None:intergenic
ATTTAAATCAATTTGAAGGA+GGG 0.554059 5.1:-69397188 None:intergenic
TCCATAGGAGCAGTGACTAA+AGG 0.557140 5.1:-69398028 None:intergenic
TTCGGATCTCCTTGCACCGG+AGG 0.558249 5.1:-69396409 None:intergenic
TGTCCAATGCCCTTTCCTTG+GGG 0.558952 5.1:-69397398 None:intergenic
CATGTTCAGCCTCCGGTGCA+AGG 0.562364 5.1:+69396400 MS.gene99367:CDS
TTGGCATCATTGGCATGAGC+AGG 0.563369 5.1:-69396376 None:intergenic
ACCTTGTCCTGCTGCTGCCG+GGG 0.566255 5.1:-69396344 None:intergenic
TTGTTCCATGCTATGCTCAG+AGG 0.567759 5.1:-69397233 None:intergenic
GAGGGATCTTGGACTGAAGC+CGG 0.570243 5.1:-69397214 None:intergenic
CATTTAAATCAATTTGAAGG+AGG 0.570848 5.1:-69397189 None:intergenic
TCCCCGGCAGCAGCAGGACA+AGG 0.572616 5.1:+69396343 MS.gene99367:CDS
TCAGGAAGTGTAGAACCGAT+TGG 0.577352 5.1:-69398139 None:intergenic
GCATCATTGGCATGAGCAGG+TGG 0.581815 5.1:-69396373 None:intergenic
TGGCACCGATATCATTGAGT+CGG 0.583638 5.1:+69397270 MS.gene99367:CDS
ACACTTTGTAGCTCAACTGA+AGG 0.589388 5.1:-69397887 None:intergenic
AAGTGTTGATGTTCCTGTGT+TGG 0.595689 5.1:+69395705 MS.gene99367:CDS
AGATCCCTCTGAGCATAGCA+TGG 0.596307 5.1:+69397228 MS.gene99367:CDS
TGGATAAAGCAACACCAACT+GGG 0.597123 5.1:-69398119 None:intergenic
GTTTGAGCATGAGAAAGAGC+TGG 0.597278 5.1:-69395748 None:intergenic
CTGTTAATTATCCAAATGGA+AGG 0.601168 5.1:+69396440 MS.gene99367:CDS
GAAGGAATTGTAGAACCGAT+TGG 0.605792 5.1:-69397980 None:intergenic
ATTAAACAAGAATGACACAT+TGG 0.608999 5.1:+69397832 MS.gene99367:CDS
ACCGATATCATTGAGTCGGG+TGG 0.615037 5.1:+69397274 MS.gene99367:CDS
GCATTCGGATCTCCTTGCAC+CGG 0.618195 5.1:-69396412 None:intergenic
CAAAGTGTGATTCCTCCTCA+AGG 0.618896 5.1:+69397902 MS.gene99367:CDS
ATCGTCGCCACAGGAAAGTG+AGG 0.619216 5.1:-69396286 None:intergenic
CAGAAGAAAGTCATGCCCCA+AGG 0.636413 5.1:+69397383 MS.gene99367:CDS
GTGACCATGTGAATCATGGT+CGG 0.644274 5.1:+69396482 MS.gene99367:CDS
AAGATGGCAGTGTCTTGAGG+AGG 0.644314 5.1:-69398067 None:intergenic
AGGCCAAATATCCAAGAACA+AGG 0.648452 5.1:+69396460 MS.gene99367:CDS
TGACAACTTGCAAACCCAGT+TGG 0.648867 5.1:+69398105 MS.gene99367:CDS
GGGCCTGGCTTTCGATAGCG+CGG 0.653594 5.1:-69396235 None:intergenic
AAGGAAAGGGCATTGGACAG+AGG 0.658250 5.1:+69397402 MS.gene99367:CDS
TTGGTTCCAATAACCAACAC+AGG 0.668427 5.1:-69395718 None:intergenic
TGTGTCTGTTGCATTCCTTG+AGG 0.673315 5.1:-69397917 None:intergenic
TGACACGCCTCACTTTCCTG+TGG 0.696402 5.1:+69396279 MS.gene99367:CDS
GATGCCGACCATGATTCACA+TGG 0.766610 5.1:-69396486 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAATAACTTTCAAACAT+AGG - chr5.1:69397466-69397485 None:intergenic 15.0%
!! AAATATTTCAAAAGAATTTG+TGG + chr5.1:69397697-69397716 MS.gene99367:CDS 15.0%
!!! AATTCTTATAGTTGTATTTT+TGG + chr5.1:69397127-69397146 MS.gene99367:intron 15.0%
!! ATTTAAATCAATTTGAAGGA+GGG - chr5.1:69397191-69397210 None:intergenic 20.0%
!! TAATTCAAATTGGATGAAAT+TGG + chr5.1:69397099-69397118 MS.gene99367:intron 20.0%
!! TGAAAGTTAAGAAAATTACT+TGG - chr5.1:69396938-69396957 None:intergenic 20.0%
!! TGTTAAACTAAATGAATAAG+TGG - chr5.1:69396606-69396625 None:intergenic 20.0%
!!! ATATATAGGTTGTTTTTGAA+GGG + chr5.1:69397644-69397663 MS.gene99367:intron 20.0%
!!! ATTTTCTTAACTTTCATGAT+TGG + chr5.1:69396942-69396961 MS.gene99367:intron 20.0%
!!! TATATATAGGTTGTTTTTGA+AGG + chr5.1:69397643-69397662 MS.gene99367:intron 20.0%
! ATTAAACAAGAATGACACAT+TGG + chr5.1:69397832-69397851 MS.gene99367:CDS 25.0%
! ATTATGATATCTTCTCGTTA+TGG + chr5.1:69396887-69396906 MS.gene99367:intron 25.0%
! CACATTCATTCTCTATATAT+AGG + chr5.1:69397630-69397649 MS.gene99367:intron 25.0%
! CATGTGGATATAATTCAAAT+TGG + chr5.1:69397089-69397108 MS.gene99367:intron 25.0%
! CATTTAAATCAATTTGAAGG+AGG - chr5.1:69397192-69397211 None:intergenic 25.0%
! GCTCATTTAAATCAATTTGA+AGG - chr5.1:69397195-69397214 None:intergenic 25.0%
! GTGATTAAACAGAAATCAAA+CGG + chr5.1:69396175-69396194 MS.gene99367:intron 25.0%
! TAAACAACGAGTGAAAAATA+TGG + chr5.1:69396020-69396039 MS.gene99367:intron 25.0%
! TAATTAACAGCATAAGCATT+CGG - chr5.1:69396430-69396449 None:intergenic 25.0%
! TATGCTGTTAATTATCCAAA+TGG + chr5.1:69396436-69396455 MS.gene99367:CDS 25.0%
! TCTACAATATTCTTAACTCT+GGG + chr5.1:69396107-69396126 MS.gene99367:intron 25.0%
! TTCAAAAAATATGATGCATG+TGG + chr5.1:69397073-69397092 MS.gene99367:intron 25.0%
! TTCTACAATATTCTTAACTC+TGG + chr5.1:69396106-69396125 MS.gene99367:intron 25.0%
! TTCTTATGCTTGCAATAATA+TGG - chr5.1:69395957-69395976 None:intergenic 25.0%
!! ATTAGAGTTTAGAATTGCAA+TGG - chr5.1:69397302-69397321 None:intergenic 25.0%
!! TCAAATTGATTTAAATGAGC+CGG + chr5.1:69397195-69397214 MS.gene99367:CDS 25.0%
!! TTAGTGTAATGTGATTTTGA+TGG + chr5.1:69395876-69395895 MS.gene99367:intron 25.0%
!!! AGGCATAATTGATTTGATTA+AGG + chr5.1:69396981-69397000 MS.gene99367:intron 25.0%
!!! ATTGTTCTTGTTGTTTAAGA+TGG - chr5.1:69398086-69398105 None:intergenic 25.0%
!!! CATGCATCATATTTTTTGAA+TGG - chr5.1:69397073-69397092 None:intergenic 25.0%
!!! TATATAGGTTGTTTTTGAAG+GGG + chr5.1:69397645-69397664 MS.gene99367:intron 25.0%
!!! TATTTTTGGTATAGAAAGCA+TGG + chr5.1:69397141-69397160 MS.gene99367:intron 25.0%
!!! TTGGTGGATAAAGTATTTTT+GGG - chr5.1:69397756-69397775 None:intergenic 25.0%
!!! TTTGGTGGATAAAGTATTTT+TGG - chr5.1:69397757-69397776 None:intergenic 25.0%
AATTGCATGAAGTGCATATA+TGG - chr5.1:69396581-69396600 None:intergenic 30.0%
ACATTCTCAACATGATTCAT+AGG - chr5.1:69395781-69395800 None:intergenic 30.0%
ACTCAATGAAGTTCATTCAA+AGG - chr5.1:69396646-69396665 None:intergenic 30.0%
AGAAGGAGAAAAAGAGAAAA+AGG + chr5.1:69397795-69397814 MS.gene99367:CDS 30.0%
CTGTTAATTATCCAAATGGA+AGG + chr5.1:69396440-69396459 MS.gene99367:CDS 30.0%
TTACTTTGATACCATTGAAG+TGG + chr5.1:69396818-69396837 MS.gene99367:intron 30.0%
TTTAATCACAGATCCATACA+CGG - chr5.1:69396164-69396183 None:intergenic 30.0%
! GAAATTTTACTGTGTTGCAT+AGG - chr5.1:69396544-69396563 None:intergenic 30.0%
! TCAACATGATTCATAGGTTT+TGG - chr5.1:69395775-69395794 None:intergenic 30.0%
! TCTCCATATCTTTCACATTT+TGG - chr5.1:69397775-69397794 None:intergenic 30.0%
! TTCTCAAGTTTGTTCATATC+AGG - chr5.1:69398160-69398179 None:intergenic 30.0%
!! ACAACTTTTATTTCCATCGA+AGG - chr5.1:69396085-69396104 None:intergenic 30.0%
!! CAACTTTTATTTCCATCGAA+GGG - chr5.1:69396084-69396103 None:intergenic 30.0%
!! TGTTGAGAATGTTTTTGTTC+AGG + chr5.1:69395789-69395808 MS.gene99367:CDS 30.0%
!!! ATTGTTTTTTTTCCCTTCGA+TGG + chr5.1:69396069-69396088 MS.gene99367:intron 30.0%
!!! CATCATTTTTTGAGTAAACC+GGG + chr5.1:69397004-69397023 MS.gene99367:intron 30.0%
!!! GCATCATTTTTTGAGTAAAC+CGG + chr5.1:69397003-69397022 MS.gene99367:intron 30.0%
AAACAGAAATCAAACGGTTC+TGG + chr5.1:69396181-69396200 MS.gene99367:intron 35.0%
CCACCAAAATGTGAAAGATA+TGG + chr5.1:69397769-69397788 MS.gene99367:CDS 35.0%
GGGATGAAAAAACATGGAAA+GGG + chr5.1:69397665-69397684 MS.gene99367:CDS 35.0%
TCACCTTGTTCTTGGATATT+TGG - chr5.1:69396466-69396485 None:intergenic 35.0%
TGTGAAAGATATGGAGAAGA+AGG + chr5.1:69397778-69397797 MS.gene99367:CDS 35.0%
TTGAAGGGGATGAAAAAACA+TGG + chr5.1:69397659-69397678 MS.gene99367:CDS 35.0%
! AATCGGTTCTACAATTCCTT+CGG + chr5.1:69397982-69398001 MS.gene99367:CDS 35.0%
! ACACTTTTCTCTTCAACACT+AGG - chr5.1:69395691-69395710 None:intergenic 35.0%
! CCATATCTTTCACATTTTGG+TGG - chr5.1:69397772-69397791 None:intergenic 35.0%
! TCTCGTTATGGTTCTGTAAT+TGG + chr5.1:69396899-69396918 MS.gene99367:intron 35.0%
! TTGGCTAGTTCATCTGATTA+AGG + chr5.1:69396961-69396980 MS.gene99367:intron 35.0%
!! CTGTAATTGGTTTGAGTAAC+TGG + chr5.1:69396912-69396931 MS.gene99367:intron 35.0%
!! TTGGTGTTGCTTTATCCAAT+CGG + chr5.1:69398124-69398143 MS.gene99367:CDS 35.0%
!!! GGATAAAGTATTTTTGGGCA+TGG - chr5.1:69397751-69397770 None:intergenic 35.0%
ACACTTTGTAGCTCAACTGA+AGG - chr5.1:69397890-69397909 None:intergenic 40.0%
AGATCCATACACGGAAAATC+AGG - chr5.1:69396155-69396174 None:intergenic 40.0%
AGGCCAAATATCCAAGAACA+AGG + chr5.1:69396460-69396479 MS.gene99367:CDS 40.0%
CTGAACATGTTTGGCATCAT+TGG - chr5.1:69396389-69396408 None:intergenic 40.0%
GAACAACAACAGGAGAAAAC+TGG + chr5.1:69397250-69397269 MS.gene99367:CDS 40.0%
GAAGGAATTGTAGAACCGAT+TGG - chr5.1:69397983-69398002 None:intergenic 40.0%
GCAACTAAAAGCCACTTCAA+TGG - chr5.1:69396832-69396851 None:intergenic 40.0%
GCGAGTCAACAAATCTGTTT+AGG + chr5.1:69396042-69396061 MS.gene99367:intron 40.0%
GGGGATGAAAAAACATGGAA+AGG + chr5.1:69397664-69397683 MS.gene99367:CDS 40.0%
TGCATGAAGTGCATATATGG+AGG - chr5.1:69396578-69396597 None:intergenic 40.0%
TGGATAAAGCAACACCAACT+GGG - chr5.1:69398122-69398141 None:intergenic 40.0%
TGGTTATTGGAACCAAGTCT+TGG + chr5.1:69395725-69395744 MS.gene99367:CDS 40.0%
TTAGTGCTCCTAAGTCTTCA+AGG + chr5.1:69395659-69395678 MS.gene99367:CDS 40.0%
TTGGATAAAGCAACACCAAC+TGG - chr5.1:69398123-69398142 None:intergenic 40.0%
TTGGTTCCAATAACCAACAC+AGG - chr5.1:69395721-69395740 None:intergenic 40.0%
! ATTTGTGTTTCTGTTGCGCT+TGG - chr5.1:69396260-69396279 None:intergenic 40.0%
! GGTGATGGCATCTTATTCTT+CGG - chr5.1:69396217-69396236 None:intergenic 40.0%
! GTCTCATGTTGTTGTTCCAT+AGG - chr5.1:69398046-69398065 None:intergenic 40.0%
! TGGATATTTGGCCTTCCATT+TGG - chr5.1:69396454-69396473 None:intergenic 40.0%
! TTTAAGATGGCAGTGTCTTG+AGG - chr5.1:69398073-69398092 None:intergenic 40.0%
! TTTGCCTGATTTTCCGTGTA+TGG + chr5.1:69396148-69396167 MS.gene99367:intron 40.0%
! TTTGTGTTTCTGTTGCGCTT+GGG - chr5.1:69396259-69396278 None:intergenic 40.0%
!! AAGATTCTAGCTTAACCCTC+TGG + chr5.1:69396736-69396755 MS.gene99367:intron 40.0%
!! AAGTGTTGATGTTCCTGTGT+TGG + chr5.1:69395705-69395724 MS.gene99367:CDS 40.0%
!! AATACCCAAAAAAGCACCAG+AGG - chr5.1:69396755-69396774 None:intergenic 40.0%
!! ATACCCAAAAAAGCACCAGA+GGG - chr5.1:69396754-69396773 None:intergenic 40.0%
!! GATGTTCCTGTGTTGGTTAT+TGG + chr5.1:69395712-69395731 MS.gene99367:CDS 40.0%
!!! TAACCCTCTGGTGCTTTTTT+GGG + chr5.1:69396748-69396767 MS.gene99367:intron 40.0%
!!! TTAACCCTCTGGTGCTTTTT+TGG + chr5.1:69396747-69396766 MS.gene99367:intron 40.0%
AAAAACATGGAAAGGGACGC+TGG + chr5.1:69397672-69397691 MS.gene99367:CDS 45.0%
AAAGTCATGCCCCAAGGAAA+GGG + chr5.1:69397389-69397408 MS.gene99367:CDS 45.0%
ACCACCCGACTCAATGATAT+CGG - chr5.1:69397278-69397297 None:intergenic 45.0%
CAAAGTGTGATTCCTCCTCA+AGG + chr5.1:69397902-69397921 MS.gene99367:CDS 45.0%
CAAGGTGACCATGTGAATCA+TGG + chr5.1:69396478-69396497 MS.gene99367:CDS 45.0%
GCATAGCATGGAACAACAAC+AGG + chr5.1:69397240-69397259 MS.gene99367:CDS 45.0%
GCATGGCTAGCAATTTGAGT+TGG - chr5.1:69397734-69397753 None:intergenic 45.0%
GGATTCTCGCCATTAGGATA+AGG - chr5.1:69396313-69396332 None:intergenic 45.0%
GGTCTCTTCCTTGAAGACTT+AGG - chr5.1:69395670-69395689 None:intergenic 45.0%
GTGACCATGTGAATCATGGT+CGG + chr5.1:69396482-69396501 MS.gene99367:CDS 45.0%
GTTTGAGCATGAGAAAGAGC+TGG - chr5.1:69395751-69395770 None:intergenic 45.0%
TCACATGGTCACCTTGTTCT+TGG - chr5.1:69396474-69396493 None:intergenic 45.0%
TCCATAGGAGCAGTGACTAA+AGG - chr5.1:69398031-69398050 None:intergenic 45.0%
TCCTTTAGTCACTGCTCCTA+TGG + chr5.1:69398027-69398046 MS.gene99367:CDS 45.0%
TGACAACTTGCAAACCCAGT+TGG + chr5.1:69398105-69398124 MS.gene99367:CDS 45.0%
TGAGAAGATGCGCAACTTGT+TGG + chr5.1:69398174-69398193 MS.gene99367:CDS 45.0%
TGTGTCTGTTGCATTCCTTG+AGG - chr5.1:69397920-69397939 None:intergenic 45.0%
TGTTCCATGCTATGCTCAGA+GGG - chr5.1:69397235-69397254 None:intergenic 45.0%
TTGTTCCATGCTATGCTCAG+AGG - chr5.1:69397236-69397255 None:intergenic 45.0%
! ATCAAAGACCTTTTCCTGCC+AGG + chr5.1:69396509-69396528 MS.gene99367:CDS 45.0%
! ATGTTGTGCTTGCTGCCAAT+CGG + chr5.1:69397965-69397984 MS.gene99367:CDS 45.0%
! TCAAAGACCTTTTCCTGCCA+GGG + chr5.1:69396510-69396529 MS.gene99367:CDS 45.0%
! TCAGGAAGTGTAGAACCGAT+TGG - chr5.1:69398142-69398161 None:intergenic 45.0%
! TTGTGTTTCTGTTGCGCTTG+GGG - chr5.1:69396258-69396277 None:intergenic 45.0%
!! TCGTTTTGTTGCTCAACCGA+AGG - chr5.1:69398001-69398020 None:intergenic 45.0%
!! TGGCACCGATATCATTGAGT+CGG + chr5.1:69397270-69397289 MS.gene99367:CDS 45.0%
AAGATGGCAGTGTCTTGAGG+AGG - chr5.1:69398070-69398089 None:intergenic 50.0%
ACCGATATCATTGAGTCGGG+TGG + chr5.1:69397274-69397293 MS.gene99367:CDS 50.0%
AGAAAGAGCTGGCCAAGACT+TGG - chr5.1:69395740-69395759 None:intergenic 50.0%
AGATCCCTCTGAGCATAGCA+TGG + chr5.1:69397228-69397247 MS.gene99367:CDS 50.0%
ATGTTTGCCCTGGCAGGAAA+AGG - chr5.1:69396520-69396539 None:intergenic 50.0%
CAGAAGAAAGTCATGCCCCA+AGG + chr5.1:69397383-69397402 MS.gene99367:CDS 50.0%
CTGTCCAATGCCCTTTCCTT+GGG - chr5.1:69397402-69397421 None:intergenic 50.0%
CTTGGGGCATGACTTTCTTC+TGG - chr5.1:69397385-69397404 None:intergenic 50.0%
GAAAGTCATGCCCCAAGGAA+AGG + chr5.1:69397388-69397407 MS.gene99367:CDS 50.0%
GAATCCTAAGCCTGTATCCC+CGG + chr5.1:69396327-69396346 MS.gene99367:CDS 50.0%
GAGTAAACCGGGTGATGCTA+GGG + chr5.1:69397015-69397034 MS.gene99367:intron 50.0%
GATGCCGACCATGATTCACA+TGG - chr5.1:69396489-69396508 None:intergenic 50.0%
GCGACGATGCCTTATCCTAA+TGG + chr5.1:69396301-69396320 MS.gene99367:CDS 50.0%
GGCTTAGGATTCTCGCCATT+AGG - chr5.1:69396319-69396338 None:intergenic 50.0%
GTCTGTTGCATTCCTTGAGG+AGG - chr5.1:69397917-69397936 None:intergenic 50.0%
TCTGTCCAATGCCCTTTCCT+TGG - chr5.1:69397403-69397422 None:intergenic 50.0%
TCTTAGTCCCTAGCATCACC+CGG - chr5.1:69397025-69397044 None:intergenic 50.0%
TGAGTAAACCGGGTGATGCT+AGG + chr5.1:69397014-69397033 MS.gene99367:intron 50.0%
TGCCAAACATGTTCAGCCTC+CGG + chr5.1:69396393-69396412 MS.gene99367:CDS 50.0%
TGCTATGCTCAGAGGGATCT+TGG - chr5.1:69397228-69397247 None:intergenic 50.0%
TGTCCAATGCCCTTTCCTTG+GGG - chr5.1:69397401-69397420 None:intergenic 50.0%
TTGCATAGGCATGTTTGCCC+TGG - chr5.1:69396530-69396549 None:intergenic 50.0%
TTGGCATCATTGGCATGAGC+AGG - chr5.1:69396379-69396398 None:intergenic 50.0%
!! AAGGAAAGGGCATTGGACAG+AGG + chr5.1:69397402-69397421 MS.gene99367:CDS 50.0%
!! GGCACCGATATCATTGAGTC+GGG + chr5.1:69397271-69397290 MS.gene99367:CDS 50.0%
AAACCTTGTCCTGCTGCTGC+CGG - chr5.1:69396349-69396368 None:intergenic 55.0%
ATAGGCATGTTTGCCCTGGC+AGG - chr5.1:69396526-69396545 None:intergenic 55.0%
ATCGTCGCCACAGGAAAGTG+AGG - chr5.1:69396289-69396308 None:intergenic 55.0%
CACCGGAGGCTGAACATGTT+TGG - chr5.1:69396398-69396417 None:intergenic 55.0%
GAGGGATCTTGGACTGAAGC+CGG - chr5.1:69397217-69397236 None:intergenic 55.0%
GCATCATTGGCATGAGCAGG+TGG - chr5.1:69396376-69396395 None:intergenic 55.0%
GCATTCGGATCTCCTTGCAC+CGG - chr5.1:69396415-69396434 None:intergenic 55.0%
TGACACGCCTCACTTTCCTG+TGG + chr5.1:69396279-69396298 MS.gene99367:CDS 55.0%
! ATGCCCCAAGGAAAGGGCAT+TGG + chr5.1:69397395-69397414 MS.gene99367:CDS 55.0%
AACCTTGTCCTGCTGCTGCC+GGG - chr5.1:69396348-69396367 None:intergenic 60.0%
CATGTTCAGCCTCCGGTGCA+AGG + chr5.1:69396400-69396419 MS.gene99367:CDS 60.0%
GGCTTTCGATAGCGCGGTGA+TGG - chr5.1:69396232-69396251 None:intergenic 60.0%
TCACCGCGCTATCGAAAGCC+AGG + chr5.1:69396232-69396251 MS.gene99367:CDS 60.0%
TTCGGATCTCCTTGCACCGG+AGG - chr5.1:69396412-69396431 None:intergenic 60.0%
TTTCTGTTGCGCTTGGGGCC+TGG - chr5.1:69396253-69396272 None:intergenic 60.0%
!! GGATAAGGCATCGTCGCCAC+AGG - chr5.1:69396298-69396317 None:intergenic 60.0%
ACCTTGTCCTGCTGCTGCCG+GGG - chr5.1:69396347-69396366 None:intergenic 65.0%
GCTGCCGGGGATACAGGCTT+AGG - chr5.1:69396334-69396353 None:intergenic 65.0%
! GGGCCTGGCTTTCGATAGCG+CGG - chr5.1:69396238-69396257 None:intergenic 65.0%
CCTGCTGCTGCCGGGGATAC+AGG - chr5.1:69396340-69396359 None:intergenic 70.0%
CCTGTATCCCCGGCAGCAGC+AGG + chr5.1:69396337-69396356 MS.gene99367:CDS 70.0%
TCCCCGGCAGCAGCAGGACA+AGG + chr5.1:69396343-69396362 MS.gene99367:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 69395637 69398210 69395637 ID=MS.gene99367
chr5.1 mRNA 69395637 69398210 69395637 ID=MS.gene99367.t1;Parent=MS.gene99367
chr5.1 exon 69395637 69395810 69395637 ID=MS.gene99367.t1.exon1;Parent=MS.gene99367.t1
chr5.1 CDS 69395637 69395810 69395637 ID=cds.MS.gene99367.t1;Parent=MS.gene99367.t1
chr5.1 exon 69396187 69396553 69396187 ID=MS.gene99367.t1.exon2;Parent=MS.gene99367.t1
chr5.1 CDS 69396187 69396553 69396187 ID=cds.MS.gene99367.t1;Parent=MS.gene99367.t1
chr5.1 exon 69397155 69397434 69397155 ID=MS.gene99367.t1.exon3;Parent=MS.gene99367.t1
chr5.1 CDS 69397155 69397434 69397155 ID=cds.MS.gene99367.t1;Parent=MS.gene99367.t1
chr5.1 exon 69397652 69398210 69397652 ID=MS.gene99367.t1.exon4;Parent=MS.gene99367.t1
chr5.1 CDS 69397652 69398210 69397652 ID=cds.MS.gene99367.t1;Parent=MS.gene99367.t1
Gene Sequence

>MS.gene99367

ATGGTGATTGCTGATAATGAGATTAGTGCTCCTAAGTCTTCAAGGAAGAGACCTAGTGTTGAAGAGAAAAGTGTTGATGTTCCTGTGTTGGTTATTGGAACCAAGTCTTGGCCAGCTCTTTCTCATGCTCAAACTCCAAAACCTATGAATCATGTTGAGAATGTTTTTGTTCAGGTTCGTTTGTTCTTTACTGTAATTTGATAGCATTGTCGTTTGAGAATGAAATGCAAAAGATCATGTTAGTGTAATGTGATTTTGATGGAGTTTTTTTGTTAAGATAAACATGTTTTTTATTGATCTATTTTTCATGATTTTAGCCATATTATTGCAAGCATAAGAATTGATTTTGATAGAGTAACAATTAAAATATGATGTGTCAGAATTAAACAACGAGTGAAAAATATGGCGAGTCAACAAATCTGTTTAGGACATATTGTTTTTTTTCCCTTCGATGGAAATAAAAGTTGTATTCTACAATATTCTTAACTCTGGGTTTGACTATTTTTTCGTGTTTGCCTGATTTTCCGTGTATGGATCTGTGATTAAACAGAAATCAAACGGTTCTGGAAACTTCAATCCGAAGAATAAGATGCCATCACCGCGCTATCGAAAGCCAGGCCCCAAGCGCAACAGAAACACAAATGACACGCCTCACTTTCCTGTGGCGACGATGCCTTATCCTAATGGCGAGAATCCTAAGCCTGTATCCCCGGCAGCAGCAGGACAAGGTTTTACTCCACCTGCTCATGCCAATGATGCCAAACATGTTCAGCCTCCGGTGCAAGGAGATCCGAATGCTTATGCTGTTAATTATCCAAATGGAAGGCCAAATATCCAAGAACAAGGTGACCATGTGAATCATGGTCGGCATCATCAAAGACCTTTTCCTGCCAGGGCAAACATGCCTATGCAACACAGTAAAATTTCTAATTAAAAATCCTCCATATATGCACTTCATGCAATTAACCACTTATTCATTTAGTTTAACATTTTTGCTGTTCTTTCACCTTTGAATGAACTTCATTGAGTTTATGTAGCGACTTTTTTGTTACTGTTACTGTTGTTGAAGATTTTAGTCTCGTTTTCTTGATTAAGTTTCAAGATTCTAGCTTAACCCTCTGGTGCTTTTTTGGGTATTGTTTTGTCTTTTATATGATTAATATCACTAATCTTCAATATTCTTACTTTGATACCATTGAAGTGGCTTTTAGTTGCAACATGATGTTTTACGATGAGTGTTTTTTATTGTCATTATGATATCTTCTCGTTATGGTTCTGTAATTGGTTTGAGTAACTGGCCAAGTAATTTTCTTAACTTTCATGATTGGCTAGTTCATCTGATTAAGGCATAATTGATTTGATTAAGGCATCATTTTTTGAGTAAACCGGGTGATGCTAGGGACTAAGAATTAAGAAGATTTAGCATATTTTTACCATTCAAAAAATATGATGCATGTGGATATAATTCAAATTGGATGAAATTGGTTTTGAATTCTTATAGTTGTATTTTTGGTATAGAAAGCATGGAAGAAAGAAAAAGTATACAATCATCCCTCCTTCAAATTGATTTAAATGAGCCGGCTTCAGTCCAAGATCCCTCTGAGCATAGCATGGAACAACAACAGGAGAAAACTGGCACCGATATCATTGAGTCGGGTGGTGCCATTGCAATTCTAAACTCTAATGATAATAACGTCACAACTCTAAACTCTGACGAAAATAACTTAATTCAAGCTGAGTCAGTACCAGAAGAAAGTCATGCCCCAAGGAAAGGGCATTGGACAGAGGATGAACACAAGTAAGTATGCAGTCTATGTTAATGTTAACCTATGTTTGAAAGTTATTTTTTTTATTTTATTGAGTTAAATATGTGTTTAGTTCTTATTAAATTACAGATTTTAACATCTATAAAATTATTCTGTTTTTTGACAATTGTTTTCATTTTATCTTTTATACAATTGTTTTCATATTATTTAGTTGAATTTGAATATTTCACATTCATTCTCTATATATAGGTTGTTTTTGAAGGGGATGAAAAAACATGGAAAGGGACGCTGGAAAAATATTTCAAAAGAATTTGTGGTGACAAAAACTCCAACTCAAATTGCTAGCCATGCCCAAAAATACTTTATCCACCAAAATGTGAAAGATATGGAGAAGAAGGAGAAAAAGAGAAAAAGGATTCACGACACAACATTAAACAAGAATGACACATTGGTCACTGTCGCTGTTGATCAAGAAGATGAGATTCCTTCAGTTGAGCTACAAAGTGTGATTCCTCCTCAAGGAATGCAACAGACACAAACACAACAGAATGAGATTTCTCGTATGTTGTGCTTGCTGCCAATCGGTTCTACAATTCCTTCGGTTGAGCAACAAAACGAGCTTACTCCTTTAGTCACTGCTCCTATGGAACAACAACATGAGACTCCTCCTCAAGACACTGCCATCTTAAACAACAAGAACAATGACAACTTGCAAACCCAGTTGGTGTTGCTTTATCCAATCGGTTCTACACTTCCTGATATGAACAAACTTGAGAAGATGCGCAACTTGTTGGCAAAAGAACTTTGA
Protein sequence

>MS.gene99367.t1

MVIADNEISAPKSSRKRPSVEEKSVDVPVLVIGTKSWPALSHAQTPKPMNHVENVFVQKSNGSGNFNPKNKMPSPRYRKPGPKRNRNTNDTPHFPVATMPYPNGENPKPVSPAAAGQGFTPPAHANDAKHVQPPVQGDPNAYAVNYPNGRPNIQEQGDHVNHGRHHQRPFPARANMPMQHKSMEERKSIQSSLLQIDLNEPASVQDPSEHSMEQQQEKTGTDIIESGGAIAILNSNDNNVTTLNSDENNLIQAESVPEESHAPRKGHWTEDEHKLFLKGMKKHGKGRWKNISKEFVVTKTPTQIASHAQKYFIHQNVKDMEKKEKKRKRIHDTTLNKNDTLVTVAVDQEDEIPSVELQSVIPPQGMQQTQTQQNEISRMLCLLPIGSTIPSVEQQNELTPLVTAPMEQQHETPPQDTAILNNKNNDNLQTQLVLLYPIGSTLPDMNKLEKMRNLLAKEL