Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99481.t1 | XP_003623323.1 | 89.3 | 159 | 11 | 1 | 1 | 159 | 1 | 153 | 6.70E-72 | 280 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99481.t1 | Q7XAQ6 | 77.4 | 62 | 14 | 0 | 37 | 98 | 39 | 100 | 9.3e-22 | 104.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99481.t1 | G7KSC0 | 89.3 | 159 | 11 | 1 | 1 | 159 | 1 | 153 | 4.8e-72 | 280.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene99481.t1 | TF | bHLH |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99481.t1 | MTR_7g069590 | 89.308 | 159 | 11 | 1 | 1 | 159 | 1 | 153 | 7.65e-89 | 256 |
| MS.gene99481.t1 | MTR_3g101810 | 61.765 | 68 | 26 | 0 | 24 | 91 | 163 | 230 | 1.45e-24 | 95.9 |
| MS.gene99481.t1 | MTR_8g093500 | 62.121 | 66 | 25 | 0 | 28 | 93 | 110 | 175 | 5.43e-23 | 92.0 |
| MS.gene99481.t1 | MTR_5g079500 | 66.038 | 53 | 18 | 0 | 39 | 91 | 225 | 277 | 4.76e-18 | 79.3 |
| MS.gene99481.t1 | MTR_5g017210 | 62.857 | 70 | 26 | 0 | 28 | 97 | 133 | 202 | 6.29e-18 | 78.2 |
| MS.gene99481.t1 | MTR_1g018090 | 48.101 | 79 | 35 | 2 | 15 | 93 | 263 | 335 | 9.48e-18 | 79.0 |
| MS.gene99481.t1 | MTR_5g005110 | 47.500 | 80 | 34 | 1 | 34 | 105 | 226 | 305 | 2.70e-17 | 77.4 |
| MS.gene99481.t1 | MTR_1g106460 | 42.105 | 95 | 44 | 2 | 26 | 112 | 235 | 326 | 4.34e-17 | 77.0 |
| MS.gene99481.t1 | MTR_1g106470 | 42.424 | 99 | 48 | 2 | 15 | 105 | 180 | 277 | 7.29e-17 | 75.9 |
| MS.gene99481.t1 | MTR_8g015450 | 56.757 | 74 | 32 | 0 | 29 | 102 | 325 | 398 | 4.97e-16 | 74.3 |
| MS.gene99481.t1 | MTR_2g100490 | 50.000 | 78 | 39 | 0 | 28 | 105 | 286 | 363 | 1.15e-15 | 73.2 |
| MS.gene99481.t1 | MTR_3g103030 | 50.769 | 65 | 28 | 1 | 29 | 93 | 230 | 290 | 1.45e-15 | 72.4 |
| MS.gene99481.t1 | MTR_4g131160 | 40.000 | 85 | 45 | 2 | 7 | 91 | 180 | 258 | 2.85e-13 | 66.6 |
| MS.gene99481.t1 | MTR_4g131160 | 40.000 | 85 | 45 | 2 | 7 | 91 | 177 | 255 | 3.03e-13 | 66.2 |
| MS.gene99481.t1 | MTR_1g106480 | 45.902 | 61 | 28 | 1 | 50 | 105 | 87 | 147 | 3.12e-12 | 61.2 |
| MS.gene99481.t1 | MTR_3g449770 | 34.677 | 124 | 63 | 5 | 25 | 139 | 302 | 416 | 4.34e-12 | 63.2 |
| MS.gene99481.t1 | MTR_4g033295 | 38.043 | 92 | 52 | 2 | 5 | 91 | 87 | 178 | 6.31e-12 | 60.8 |
| MS.gene99481.t1 | MTR_4g033295 | 38.043 | 92 | 52 | 2 | 5 | 91 | 87 | 178 | 1.01e-11 | 60.5 |
| MS.gene99481.t1 | MTR_1g060700 | 41.935 | 62 | 36 | 0 | 39 | 100 | 354 | 415 | 1.65e-11 | 61.6 |
| MS.gene99481.t1 | MTR_1g080890 | 49.057 | 53 | 27 | 0 | 39 | 91 | 200 | 252 | 2.18e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99481.t1 | AT5G01305 | 65.854 | 82 | 27 | 1 | 22 | 103 | 28 | 108 | 1.78e-34 | 119 |
| MS.gene99481.t1 | AT3G21330 | 47.727 | 88 | 46 | 0 | 28 | 115 | 265 | 352 | 5.37e-22 | 90.9 |
| MS.gene99481.t1 | AT5G43175 | 42.553 | 94 | 46 | 1 | 26 | 111 | 126 | 219 | 2.87e-20 | 84.0 |
| MS.gene99481.t1 | AT4G00120 | 53.030 | 66 | 31 | 0 | 28 | 93 | 108 | 173 | 6.32e-20 | 82.4 |
| MS.gene99481.t1 | AT1G27740 | 43.878 | 98 | 48 | 3 | 2 | 93 | 132 | 228 | 6.41e-18 | 78.2 |
| MS.gene99481.t1 | AT4G33880 | 56.667 | 60 | 26 | 0 | 34 | 93 | 268 | 327 | 7.67e-18 | 79.3 |
| MS.gene99481.t1 | AT5G67060 | 63.235 | 68 | 25 | 0 | 24 | 91 | 114 | 181 | 8.56e-18 | 77.8 |
| MS.gene99481.t1 | AT5G67060 | 63.235 | 68 | 25 | 0 | 24 | 91 | 102 | 169 | 8.71e-18 | 77.4 |
| MS.gene99481.t1 | AT3G50330 | 63.235 | 68 | 25 | 0 | 24 | 91 | 111 | 178 | 1.53e-17 | 76.6 |
| MS.gene99481.t1 | AT5G09750 | 66.667 | 66 | 22 | 0 | 28 | 93 | 115 | 180 | 3.02e-17 | 75.9 |
| MS.gene99481.t1 | AT2G14760 | 55.000 | 60 | 27 | 0 | 34 | 93 | 239 | 298 | 1.67e-16 | 75.5 |
| MS.gene99481.t1 | AT1G66470 | 54.839 | 62 | 28 | 0 | 30 | 91 | 193 | 254 | 1.74e-16 | 75.1 |
| MS.gene99481.t1 | AT5G37800 | 60.784 | 51 | 20 | 0 | 41 | 91 | 210 | 260 | 2.56e-16 | 74.7 |
| MS.gene99481.t1 | AT4G30980 | 42.857 | 77 | 40 | 1 | 15 | 91 | 117 | 189 | 3.88e-14 | 68.6 |
| MS.gene99481.t1 | AT4G30980 | 42.857 | 77 | 40 | 1 | 15 | 91 | 165 | 237 | 5.01e-14 | 68.6 |
| MS.gene99481.t1 | AT2G24260 | 52.727 | 55 | 26 | 0 | 39 | 93 | 42 | 96 | 7.26e-14 | 67.0 |
| MS.gene99481.t1 | AT2G24260 | 54.717 | 53 | 24 | 0 | 39 | 91 | 145 | 197 | 2.21e-13 | 66.6 |
| MS.gene99481.t1 | AT2G14760 | 45.205 | 73 | 27 | 1 | 34 | 93 | 239 | 311 | 3.66e-13 | 66.2 |
| MS.gene99481.t1 | AT5G58010 | 41.772 | 79 | 42 | 1 | 13 | 91 | 84 | 158 | 2.16e-12 | 63.5 |
| MS.gene99481.t1 | AT4G28800 | 34.021 | 97 | 58 | 2 | 22 | 112 | 236 | 332 | 9.62e-11 | 59.3 |
Find 23 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAAAGAGAGAGGAGAAAA+AGG | 0.204891 | 7.1:+32530017 | MS.gene99481:CDS |
| ATTAACTTTGTTGATAATAT+TGG | 0.286179 | 7.1:+32530252 | MS.gene99481:CDS |
| TGTAACAACACTTATTCTTC+AGG | 0.295525 | 7.1:+32529988 | MS.gene99481:CDS |
| TCTCTAGCTGCAACACTTTG+TGG | 0.303892 | 7.1:-32530084 | None:intergenic |
| GATCCCTGGTGGAAGTAAAT+TGG | 0.340425 | 7.1:+32530152 | MS.gene99481:CDS |
| AAGTTTCACTGAACTTCCTT+TGG | 0.367586 | 7.1:-32530052 | None:intergenic |
| TCATTTGAAGAATAATGTTC+AGG | 0.411947 | 7.1:-32530294 | None:intergenic |
| AGTTTCTTAAGAAACAGATT+TGG | 0.417653 | 7.1:+32530214 | MS.gene99481:CDS |
| GTAACAACACTTATTCTTCA+GGG | 0.451744 | 7.1:+32529989 | MS.gene99481:CDS |
| TTTCATAGTGGCATCAAAGA+TGG | 0.476710 | 7.1:-32530412 | None:intergenic |
| GAAGACATAGAATCAGTGAT+AGG | 0.495589 | 7.1:+32530109 | MS.gene99481:CDS |
| ATTGGACACTGTTTCAATGT+TGG | 0.507524 | 7.1:+32530170 | MS.gene99481:CDS |
| GAATAAGTGTTGTTACAAAA+CGG | 0.528040 | 7.1:-32529982 | None:intergenic |
| AGGGTCATGTTTAAAGAGAG+AGG | 0.558108 | 7.1:+32530008 | MS.gene99481:CDS |
| TTGAAGAATAATGTTCAGGA+AGG | 0.572521 | 7.1:-32530290 | None:intergenic |
| GTCTAATTAATATTTCATAG+TGG | 0.574341 | 7.1:-32530424 | None:intergenic |
| TGAAAACAGAACTGCTCTAG+TGG | 0.577339 | 7.1:-32530369 | None:intergenic |
| GTGTCCAATTTACTTCCACC+AGG | 0.595621 | 7.1:-32530156 | None:intergenic |
| ATAGACTCAAATGAACCTGA+AGG | 0.610939 | 7.1:-32530327 | None:intergenic |
| ATTCTTCAGAGTATGATCCC+TGG | 0.631016 | 7.1:+32530138 | MS.gene99481:CDS |
| CTTCAGAGTATGATCCCTGG+TGG | 0.671280 | 7.1:+32530141 | MS.gene99481:CDS |
| AACTGCTCTAGTGGTAACTG+TGG | 0.694715 | 7.1:-32530360 | None:intergenic |
| TGTCCAATTTACTTCCACCA+GGG | 0.712775 | 7.1:-32530155 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTAACTTTGTTGATAATAT+TGG | + | chr7.1:32530252-32530271 | MS.gene99481:CDS | 15.0% |
| !! | AATGATAAAAATATTCCTTC+AGG | + | chr7.1:32530312-32530331 | MS.gene99481:CDS | 20.0% |
| ! | AGTTTCTTAAGAAACAGATT+TGG | + | chr7.1:32530214-32530233 | MS.gene99481:CDS | 25.0% |
| ! | TCATTTGAAGAATAATGTTC+AGG | - | chr7.1:32530297-32530316 | None:intergenic | 25.0% |
| !! | GAATAAGTGTTGTTACAAAA+CGG | - | chr7.1:32529985-32530004 | None:intergenic | 25.0% |
| GTAACAACACTTATTCTTCA+GGG | + | chr7.1:32529989-32530008 | MS.gene99481:CDS | 30.0% | |
| TGTAACAACACTTATTCTTC+AGG | + | chr7.1:32529988-32530007 | MS.gene99481:CDS | 30.0% | |
| TTAAAGAGAGAGGAGAAAAA+GGG | + | chr7.1:32530018-32530037 | MS.gene99481:CDS | 30.0% | |
| TTGAAGAATAATGTTCAGGA+AGG | - | chr7.1:32530293-32530312 | None:intergenic | 30.0% | |
| TTTAAAGAGAGAGGAGAAAA+AGG | + | chr7.1:32530017-32530036 | MS.gene99481:CDS | 30.0% | |
| AAGTTTCACTGAACTTCCTT+TGG | - | chr7.1:32530055-32530074 | None:intergenic | 35.0% | |
| ATAGACTCAAATGAACCTGA+AGG | - | chr7.1:32530330-32530349 | None:intergenic | 35.0% | |
| ATTGGACACTGTTTCAATGT+TGG | + | chr7.1:32530170-32530189 | MS.gene99481:CDS | 35.0% | |
| GAAGACATAGAATCAGTGAT+AGG | + | chr7.1:32530109-32530128 | MS.gene99481:CDS | 35.0% | |
| TTTCATAGTGGCATCAAAGA+TGG | - | chr7.1:32530415-32530434 | None:intergenic | 35.0% | |
| ! | AAGGGTGTGAAAAAATCCAA+AGG | + | chr7.1:32530036-32530055 | MS.gene99481:CDS | 35.0% |
| AGGGTCATGTTTAAAGAGAG+AGG | + | chr7.1:32530008-32530027 | MS.gene99481:CDS | 40.0% | |
| ATTCTTCAGAGTATGATCCC+TGG | + | chr7.1:32530138-32530157 | MS.gene99481:CDS | 40.0% | |
| TGAAAACAGAACTGCTCTAG+TGG | - | chr7.1:32530372-32530391 | None:intergenic | 40.0% | |
| TGTCCAATTTACTTCCACCA+GGG | - | chr7.1:32530158-32530177 | None:intergenic | 40.0% | |
| ! | CTAGAGCAGTTCTGTTTTCA+AGG | + | chr7.1:32530372-32530391 | MS.gene99481:CDS | 40.0% |
| AACTGCTCTAGTGGTAACTG+TGG | - | chr7.1:32530363-32530382 | None:intergenic | 45.0% | |
| GATCCCTGGTGGAAGTAAAT+TGG | + | chr7.1:32530152-32530171 | MS.gene99481:CDS | 45.0% | |
| GTGTCCAATTTACTTCCACC+AGG | - | chr7.1:32530159-32530178 | None:intergenic | 45.0% | |
| TCTCTAGCTGCAACACTTTG+TGG | - | chr7.1:32530087-32530106 | None:intergenic | 45.0% | |
| CTTCAGAGTATGATCCCTGG+TGG | + | chr7.1:32530141-32530160 | MS.gene99481:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 32529961 | 32530440 | 32529961 | ID=MS.gene99481 |
| chr7.1 | mRNA | 32529961 | 32530440 | 32529961 | ID=MS.gene99481.t1;Parent=MS.gene99481 |
| chr7.1 | exon | 32529961 | 32530440 | 32529961 | ID=MS.gene99481.t1.exon1;Parent=MS.gene99481.t1 |
| chr7.1 | CDS | 32529961 | 32530440 | 32529961 | ID=cds.MS.gene99481.t1;Parent=MS.gene99481.t1 |
| Gene Sequence |
| Protein sequence |
