AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99484

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99484.t1 MTR_7g069660 93.503 354 17 2 1 350 1 352 0.0 647
MS.gene99484.t1 MTR_2g084230 62.069 116 40 2 18 132 50 162 1.56e-37 137
MS.gene99484.t1 MTR_8g086410 57.983 119 43 2 21 132 56 174 3.51e-37 139
MS.gene99484.t1 MTR_2g084230 62.069 116 40 2 18 132 50 162 3.58e-37 137
MS.gene99484.t1 MTR_8g086410 57.983 119 43 2 21 132 58 176 3.66e-37 139
MS.gene99484.t1 MTR_2g023580 81.159 69 13 0 55 123 43 111 7.04e-35 128
MS.gene99484.t1 MTR_8g086410 54.762 126 43 3 21 132 58 183 1.22e-34 132
MS.gene99484.t1 MTR_2g016220 71.951 82 23 0 50 131 72 153 8.85e-34 128
MS.gene99484.t1 MTR_2g016220 71.951 82 23 0 50 131 72 153 9.23e-34 128
MS.gene99484.t1 MTR_2g016220 68.605 86 23 1 50 131 72 157 1.22e-31 122
MS.gene99484.t1 MTR_1g112370 72.727 66 18 0 58 123 13 78 2.42e-28 113
MS.gene99484.t1 MTR_0223s0040 65.278 72 25 0 52 123 3 74 5.58e-28 110
MS.gene99484.t1 MTR_6g071625 55.294 85 31 1 66 150 201 278 4.20e-23 99.0
MS.gene99484.t1 MTR_8g066310 44.144 111 58 1 35 141 137 247 1.82e-22 97.1
MS.gene99484.t1 MTR_1g021520 59.211 76 29 1 66 141 125 198 2.48e-22 95.9
MS.gene99484.t1 MTR_5g029470 56.098 82 35 1 61 141 212 293 5.94e-22 96.3
MS.gene99484.t1 MTR_3g450310 42.105 114 56 3 49 162 1 104 9.24e-22 92.4
MS.gene99484.t1 MTR_3g064840 64.179 67 23 1 61 126 267 333 3.04e-21 94.7
MS.gene99484.t1 MTR_7g098250 64.286 56 20 0 68 123 263 318 6.30e-19 88.2
MS.gene99484.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 1.16e-18 85.5
MS.gene99484.t1 MTR_7g093030 49.367 79 39 1 63 140 208 286 1.85e-18 84.7
MS.gene99484.t1 MTR_1g080330 62.500 56 21 0 68 123 176 231 1.86e-18 84.7
MS.gene99484.t1 MTR_6g032990 66.038 53 18 0 68 120 43 95 1.94e-18 85.9
MS.gene99484.t1 MTR_1g080330 62.500 56 21 0 68 123 176 231 2.25e-18 85.9
MS.gene99484.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 2.28e-18 85.1
MS.gene99484.t1 MTR_2g086450 62.500 56 21 0 68 123 46 101 2.95e-18 85.5
MS.gene99484.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 3.07e-18 85.9
MS.gene99484.t1 MTR_5g027440 62.069 58 22 0 63 120 21 78 3.23e-18 84.0
MS.gene99484.t1 MTR_7g093030 49.367 79 39 1 63 140 208 286 4.72e-18 84.7
MS.gene99484.t1 MTR_7g115530 62.500 56 21 0 68 123 219 274 5.52e-18 84.3
MS.gene99484.t1 MTR_7g088070 55.224 67 30 0 59 125 42 108 1.27e-17 82.0
MS.gene99484.t1 MTR_7g088070 55.224 67 30 0 59 125 42 108 1.43e-17 82.0
MS.gene99484.t1 MTR_1g090670 60.714 56 22 0 68 123 45 100 7.79e-17 80.1
MS.gene99484.t1 MTR_2g027800 60.714 56 22 0 68 123 40 95 8.62e-17 80.5
MS.gene99484.t1 MTR_7g115530 63.462 52 19 0 68 119 219 270 1.05e-16 79.7
MS.gene99484.t1 MTR_2g027800 62.264 53 20 0 68 120 40 92 1.30e-16 79.7
MS.gene99484.t1 MTR_7g115530 60.714 56 22 0 68 123 219 274 1.33e-16 79.3
MS.gene99484.t1 MTR_5g041350 56.604 53 23 0 68 120 16 68 1.80e-16 79.7
MS.gene99484.t1 MTR_2g027860 61.404 57 22 0 69 125 51 107 2.16e-16 79.3
MS.gene99484.t1 MTR_1g053830 56.364 55 24 0 69 123 20 74 2.36e-16 74.3
MS.gene99484.t1 MTR_1g053835 52.055 73 34 1 69 140 260 332 2.92e-16 79.7
MS.gene99484.t1 MTR_6g444980 55.357 56 25 0 68 123 36 91 3.27e-15 76.3
MS.gene99484.t1 MTR_6g444980 55.357 56 25 0 68 123 36 91 3.96e-15 75.9
MS.gene99484.t1 MTR_4g081710 55.357 56 25 0 68 123 18 73 4.81e-15 75.5
MS.gene99484.t1 MTR_7g068600 56.667 60 23 1 69 125 15 74 3.92e-13 68.6
MS.gene99484.t1 MTR_4g021790 51.786 56 26 1 65 120 166 220 1.44e-12 67.4
MS.gene99484.t1 MTR_1g053800 54.717 53 24 0 69 121 179 231 1.79e-12 67.8
MS.gene99484.t1 MTR_4g086835 45.205 73 31 1 57 120 255 327 3.32e-12 67.4
MS.gene99484.t1 MTR_4g064730 48.684 76 37 2 46 120 109 183 9.23e-12 65.5
MS.gene99484.t1 MTR_2g450070 39.316 117 57 6 66 178 212 318 1.13e-11 66.2
MS.gene99484.t1 MTR_2g450070 39.316 117 57 6 66 178 212 318 1.16e-11 66.2
MS.gene99484.t1 MTR_4g113140 55.769 52 23 0 69 120 237 288 1.30e-11 65.9
MS.gene99484.t1 MTR_7g093010 49.231 65 33 0 69 133 215 279 2.37e-11 64.7
MS.gene99484.t1 MTR_4g086835 45.205 73 30 2 57 120 255 326 7.55e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99484.t1 AT2G38300 42.025 326 153 14 44 350 32 340 3.07e-57 189
MS.gene99484.t1 AT2G40260 63.889 108 37 1 50 157 65 170 3.78e-39 144
MS.gene99484.t1 AT2G42660 78.788 66 14 0 59 124 42 107 2.00e-31 119
MS.gene99484.t1 AT2G02060 65.432 81 28 0 40 120 2 82 1.51e-29 114
MS.gene99484.t1 AT2G02060 65.432 81 28 0 40 120 2 82 1.51e-29 114
MS.gene99484.t1 AT4G04605 59.302 86 33 1 55 138 2 87 1.63e-28 110
MS.gene99484.t1 AT1G14600 68.657 67 21 0 59 125 15 81 1.97e-28 111
MS.gene99484.t1 AT4G04580 58.209 67 28 0 57 123 4 70 3.60e-23 94.7
MS.gene99484.t1 AT5G42630 67.213 61 20 0 66 126 104 164 2.31e-22 94.4
MS.gene99484.t1 AT5G42630 67.213 61 20 0 66 126 104 164 2.43e-22 95.5
MS.gene99484.t1 AT1G32240 54.878 82 36 1 61 141 206 287 9.06e-22 95.5
MS.gene99484.t1 AT1G32240 54.878 82 36 1 61 141 206 287 1.16e-21 95.1
MS.gene99484.t1 AT4G04555 60.000 65 26 0 59 123 6 70 1.70e-21 88.6
MS.gene99484.t1 AT5G16560 58.108 74 30 1 61 133 212 285 5.88e-21 93.6
MS.gene99484.t1 AT4G17695 62.295 61 23 0 66 126 163 223 4.05e-20 90.1
MS.gene99484.t1 AT5G45580 62.069 58 22 0 63 120 3 60 2.00e-18 84.3
MS.gene99484.t1 AT5G45580 62.069 58 22 0 63 120 19 76 2.45e-18 84.3
MS.gene99484.t1 AT3G04030 58.929 56 23 0 68 123 46 101 1.67e-17 83.2
MS.gene99484.t1 AT3G04030 58.929 56 23 0 68 123 46 101 1.89e-17 83.2
MS.gene99484.t1 AT3G04450 49.333 75 30 1 57 123 220 294 2.15e-17 82.0
MS.gene99484.t1 AT3G04450 49.333 75 30 1 57 123 180 254 2.46e-17 82.8
MS.gene99484.t1 AT3G04450 49.333 75 30 1 57 123 180 254 2.46e-17 82.8
MS.gene99484.t1 AT3G04450 49.333 75 30 1 57 123 220 294 2.52e-17 83.2
MS.gene99484.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.21e-17 82.4
MS.gene99484.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.21e-17 82.4
MS.gene99484.t1 AT5G29000 63.636 55 20 0 69 123 190 244 3.26e-17 82.4
MS.gene99484.t1 AT5G29000 63.636 55 20 0 69 123 190 244 3.26e-17 82.4
MS.gene99484.t1 AT4G28610 61.818 55 21 0 69 123 227 281 3.55e-17 82.4
MS.gene99484.t1 AT5G29000 63.636 55 20 0 69 123 233 287 3.65e-17 81.6
MS.gene99484.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.79e-17 82.4
MS.gene99484.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.79e-17 82.4
MS.gene99484.t1 AT5G18240 58.929 56 23 0 68 123 46 101 3.85e-17 82.4
MS.gene99484.t1 AT5G29000 63.636 55 20 0 69 123 233 287 4.58e-17 82.0
MS.gene99484.t1 AT2G01060 61.404 57 22 0 69 125 17 73 9.75e-17 79.7
MS.gene99484.t1 AT3G12730 57.143 56 24 0 68 123 24 79 1.35e-16 78.6
MS.gene99484.t1 AT4G13640 56.604 53 23 0 68 120 38 90 5.20e-16 77.8
MS.gene99484.t1 AT4G13640 56.604 53 23 0 68 120 38 90 5.55e-16 77.8
MS.gene99484.t1 AT3G24120 56.604 53 23 0 68 120 42 94 6.92e-16 77.4
MS.gene99484.t1 AT3G24120 56.604 53 23 0 68 120 42 94 7.16e-16 77.4
MS.gene99484.t1 AT3G13040 59.649 57 23 0 69 125 243 299 7.46e-16 79.0
MS.gene99484.t1 AT3G13040 59.649 57 23 0 69 125 243 299 7.46e-16 79.0
MS.gene99484.t1 AT3G13040 59.649 57 23 0 69 125 243 299 7.46e-16 79.0
MS.gene99484.t1 AT1G79430 55.357 56 25 0 68 123 35 90 1.86e-15 77.0
MS.gene99484.t1 AT2G20400 57.895 57 24 0 69 125 233 289 1.92e-15 77.4
MS.gene99484.t1 AT2G20400 57.895 57 24 0 69 125 233 289 1.93e-15 77.4
MS.gene99484.t1 AT5G06800 54.098 61 28 0 69 129 194 254 3.26e-15 75.5
MS.gene99484.t1 AT3G04030 57.143 56 23 1 68 123 46 100 4.93e-15 75.9
MS.gene99484.t1 AT1G69580 55.357 56 25 0 68 123 35 90 6.53e-15 75.1
MS.gene99484.t1 AT1G69580 55.357 56 25 0 68 123 35 90 6.83e-15 75.1
MS.gene99484.t1 AT5G06800 54.098 61 28 0 69 129 194 254 7.92e-15 75.5
MS.gene99484.t1 AT5G06800 54.098 61 28 0 69 129 194 254 8.00e-15 75.1
MS.gene99484.t1 AT2G06020 36.522 115 62 3 15 120 27 139 3.99e-14 72.4
MS.gene99484.t1 AT5G59570 45.455 88 44 3 35 120 108 193 3.17e-13 69.7
MS.gene99484.t1 AT5G59570 45.455 88 44 3 35 120 108 193 3.17e-13 69.7
MS.gene99484.t1 AT2G40260 58.929 56 21 1 102 157 1 54 1.87e-12 67.4
MS.gene99484.t1 AT1G25550 59.184 49 20 0 72 120 214 262 2.26e-12 67.8
MS.gene99484.t1 AT2G03500 57.143 49 21 0 72 120 238 286 1.14e-11 65.9
MS.gene99484.t1 AT1G25550 46.269 67 32 1 72 138 214 276 1.48e-11 64.7
MS.gene99484.t1 AT3G46640 54.545 55 24 1 66 120 143 196 2.00e-11 64.7
MS.gene99484.t1 AT3G46640 54.545 55 24 1 66 120 143 196 2.00e-11 64.7
MS.gene99484.t1 AT3G46640 54.545 55 24 1 66 120 143 196 2.91e-11 64.3
MS.gene99484.t1 AT3G62670 43.056 72 34 2 53 120 200 268 3.43e-11 64.7

Find 58 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCAATTGAAGAACCAATTT+TGG 0.129269 7.1:-32482031 None:intergenic
TGTTGATAGTTCTCTAAAAT+TGG 0.214021 7.1:-32483110 None:intergenic
CTTCAATTGATGAATATAAA+AGG 0.228877 7.1:+32482043 MS.gene99484:CDS
GATATTGATGTTGCTATTAT+TGG 0.260085 7.1:-32483056 None:intergenic
GAAAGGAGTCAATATTTCTT+TGG 0.270906 7.1:+32483468 MS.gene99484:CDS
CTATATGATGATGTTTGATT+TGG 0.300830 7.1:-32482453 None:intergenic
GGAAATTATCATTTGGTTCA+TGG 0.310661 7.1:-32483141 None:intergenic
GAAAAGAAAAGCTTCAAATA+TGG 0.323316 7.1:+32483279 MS.gene99484:CDS
TCATGTGAAGAGTCATTTAC+AGG 0.340032 7.1:+32482078 MS.gene99484:CDS
TGTAGGAGCAACTCCAAAAT+TGG 0.369328 7.1:+32482018 MS.gene99484:intron
TAGAATATGTCTCATTCATA+TGG 0.376004 7.1:-32483497 None:intergenic
AGAAGAACAAGATAGTTCTA+AGG 0.388489 7.1:+32483438 MS.gene99484:CDS
GCCTTGAAGCATGGGAAGTT+GGG 0.390047 7.1:-32482424 None:intergenic
ATTAATTGAAACACTTGCCT+TGG 0.400061 7.1:-32481507 None:intergenic
TATCACTAAAATTAAATTCA+AGG 0.404007 7.1:+32483317 MS.gene99484:CDS
GATCATCTCTTAAAGATTCA+TGG 0.404027 7.1:-32483177 None:intergenic
AATGAGAGGAAATTATCATT+TGG 0.404536 7.1:-32483148 None:intergenic
TAAAATTGGATGAATCCATT+TGG 0.405764 7.1:-32483096 None:intergenic
CTTCATGCTGTGCAAAGACT+TGG 0.407098 7.1:+32481707 MS.gene99484:CDS
ACCCAACTTCCCATGCTTCA+AGG 0.422458 7.1:+32482423 MS.gene99484:CDS
GAATCTAGAGCTGAGTTCTA+TGG 0.429313 7.1:+32483016 MS.gene99484:CDS
CAAATCAAACATCATCATAT+AGG 0.434083 7.1:+32482454 MS.gene99484:CDS
TTTACAGATGTGGATCATAT+GGG 0.447788 7.1:+32482942 MS.gene99484:intron
AATCATCTTATGTTTGCAAA+AGG 0.448039 7.1:+32483410 MS.gene99484:CDS
TAACAGTGTTGGCTGATCAT+AGG 0.450325 7.1:+32482361 MS.gene99484:intron
TAATAGCAACATCAATATCC+AGG 0.451694 7.1:+32483060 MS.gene99484:CDS
GAAATGAGATCTTGTGCTCT+TGG 0.453092 7.1:-32483211 None:intergenic
AGAAAACGAAAATGAAGATC+AGG 0.462194 7.1:-32481686 None:intergenic
CCAACGTAGCCTAGGCAACT+TGG 0.470061 7.1:-32481660 None:intergenic
AGCCTTGAAGCATGGGAAGT+TGG 0.470813 7.1:-32482425 None:intergenic
GGAGTATAGCCTTGAAGCAT+GGG 0.488714 7.1:-32482432 None:intergenic
ATGAGAGAGGAAGAAACATA+TGG 0.490280 7.1:+32481473 MS.gene99484:CDS
TTCAAATATGGATCTTGAGT+TGG 0.495575 7.1:+32483291 MS.gene99484:CDS
CATGCTGTGCAAAGACTTGG+TGG 0.512111 7.1:+32481710 MS.gene99484:CDS
TGGAGTATAGCCTTGAAGCA+TGG 0.512500 7.1:-32482433 None:intergenic
GGCAACTTGGATCTAACATA+AGG 0.518664 7.1:-32481647 None:intergenic
ATGTTAGATCCAAGTTGCCT+AGG 0.523365 7.1:+32481651 MS.gene99484:CDS
AGTTCTAAGGAGCAAGAGAA+AGG 0.525178 7.1:+32483451 MS.gene99484:CDS
AAAGTAGTTGACACAAATCA+AGG 0.545661 7.1:+32482234 MS.gene99484:CDS
TGGTGATCATAAATGTATAG+AGG 0.550152 7.1:+32483345 MS.gene99484:CDS
TTTGGAAAATACTATGACCC+TGG 0.552012 7.1:-32483078 None:intergenic
TCAGGAGTCCAACGTAGCCT+AGG 0.559435 7.1:-32481668 None:intergenic
CCAAGTTGCCTAGGCTACGT+TGG 0.561524 7.1:+32481660 MS.gene99484:CDS
TCTTGCCATGTATGAAAACA+TGG 0.579727 7.1:+32482973 MS.gene99484:CDS
CATAGGCTTCTTGTAGACAA+TGG 0.581433 7.1:+32482378 MS.gene99484:CDS
CTAACATAAGGCCTCACTGA+TGG 0.585574 7.1:-32481635 None:intergenic
TTACAGATGTGGATCATATG+GGG 0.594097 7.1:+32482943 MS.gene99484:intron
AAATTAAATTCAAGGAGCAA+TGG 0.605129 7.1:+32483325 MS.gene99484:CDS
TAACTTGCCTGAAAATCAAG+AGG 0.607982 7.1:+32483240 MS.gene99484:CDS
ATTCATGGCCACAAAATGAG+AGG 0.620365 7.1:-32483162 None:intergenic
GATGTGCAAAAGAACAACAA+TGG 0.621747 7.1:+32481563 MS.gene99484:CDS
AATAGCAACATCAATATCCA+GGG 0.624382 7.1:+32483061 MS.gene99484:CDS
GATGATTGTGAAATTAGTGA+AGG 0.638841 7.1:+32481533 MS.gene99484:CDS
ATATGGATCAGAATGTTCCA+AGG 0.643313 7.1:+32481490 MS.gene99484:CDS
GTGCAAAAGAACAACAATGG+AGG 0.658548 7.1:+32481566 MS.gene99484:CDS
TAGCTAGATGAATATGAGAG+AGG 0.659511 7.1:+32481460 None:intergenic
ATGCTGTGCAAAGACTTGGT+GGG 0.679037 7.1:+32481711 MS.gene99484:CDS
GTGAGAAGAAACCATCAGTG+AGG 0.735513 7.1:+32481624 MS.gene99484:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTTTTAATTTTATTTTT+TGG - chr7.1:32482758-32482777 None:intergenic 0.0%
!!! AATTAACCTTTTTTTTTTTA+TGG + chr7.1:32482494-32482513 MS.gene99484:intron 10.0%
!! AATAAGTGATTACATAATTA+GGG + chr7.1:32482296-32482315 MS.gene99484:intron 15.0%
!! TAAAAAGTCATAGATAAATA+TGG + chr7.1:32482101-32482120 MS.gene99484:intron 15.0%
!! TATCACTAAAATTAAATTCA+AGG + chr7.1:32483317-32483336 MS.gene99484:CDS 15.0%
!!! TTTTTTTTTTTTTACAGATG+TGG + chr7.1:32482932-32482951 MS.gene99484:intron 15.0%
!! CAATAAGTGATTACATAATT+AGG + chr7.1:32482295-32482314 MS.gene99484:intron 20.0%
!! CATTCTCCATAAAAAAAAAA+AGG - chr7.1:32482503-32482522 None:intergenic 20.0%
!! CTTCAATTGATGAATATAAA+AGG + chr7.1:32482043-32482062 MS.gene99484:CDS 20.0%
!! GAAAAATTGAAATCTAGAAA+AGG - chr7.1:32481874-32481893 None:intergenic 20.0%
!!! AAGACCTTTTTTTATTCATA+GGG + chr7.1:32482646-32482665 MS.gene99484:intron 20.0%
!!! CTAGATTTCAATTTTTCATA+GGG + chr7.1:32481877-32481896 MS.gene99484:intron 20.0%
!!! TAGATTTCAATTTTTCATAG+GGG + chr7.1:32481878-32481897 MS.gene99484:intron 20.0%
!!! TCTAGATTTCAATTTTTCAT+AGG + chr7.1:32481876-32481895 MS.gene99484:intron 20.0%
!!! TTTTAAACGTAAATGACTTA+AGG - chr7.1:32482833-32482852 None:intergenic 20.0%
!!! TTTTATTTTTTGGTCAAGTT+CGG - chr7.1:32482748-32482767 None:intergenic 20.0%
! AAATTAAATTCAAGGAGCAA+TGG + chr7.1:32483325-32483344 MS.gene99484:CDS 25.0%
! AATCATCTTATGTTTGCAAA+AGG + chr7.1:32483410-32483429 MS.gene99484:CDS 25.0%
! AATGAGAGGAAATTATCATT+TGG - chr7.1:32483151-32483170 None:intergenic 25.0%
! AGTTATCAAAACAAGTACAA+AGG + chr7.1:32482705-32482724 MS.gene99484:intron 25.0%
! ATACATATATGTTATGTGTG+TGG - chr7.1:32481751-32481770 None:intergenic 25.0%
! CAAATCAAACATCATCATAT+AGG + chr7.1:32482454-32482473 MS.gene99484:CDS 25.0%
! GAAAAGAAAAGCTTCAAATA+TGG + chr7.1:32483279-32483298 MS.gene99484:CDS 25.0%
! GTAAATGACTTAAGGAAATT+TGG - chr7.1:32482825-32482844 None:intergenic 25.0%
! GTTATCAAAACAAGTACAAA+GGG + chr7.1:32482706-32482725 MS.gene99484:intron 25.0%
! TAAAATTGGATGAATCCATT+TGG - chr7.1:32483099-32483118 None:intergenic 25.0%
! TGATCCCTATGAATAAAAAA+AGG - chr7.1:32482653-32482672 None:intergenic 25.0%
! TGTTATTGTCTTAGAATTGA+AGG + chr7.1:32482906-32482925 MS.gene99484:intron 25.0%
! TTGTATATATGTAACAGTGT+TGG + chr7.1:32482350-32482369 MS.gene99484:intron 25.0%
!! ATCAATTGAAGAACCAATTT+TGG - chr7.1:32482034-32482053 None:intergenic 25.0%
!! CTATATGATGATGTTTGATT+TGG - chr7.1:32482456-32482475 None:intergenic 25.0%
!! GATATTGATGTTGCTATTAT+TGG - chr7.1:32483059-32483078 None:intergenic 25.0%
!! TTAATGATTTTCTTGTGTGT+AGG + chr7.1:32482001-32482020 MS.gene99484:intron 25.0%
!!! CAAGACCTTTTTTTATTCAT+AGG + chr7.1:32482645-32482664 MS.gene99484:intron 25.0%
!!! TAAAAAAAGGTCTTGTTAAC+CGG - chr7.1:32482640-32482659 None:intergenic 25.0%
!!! TGTTGATAGTTCTCTAAAAT+TGG - chr7.1:32483113-32483132 None:intergenic 25.0%
AAAGCTAGCTAGCTATATAT+AGG - chr7.1:32482133-32482152 None:intergenic 30.0%
AAAGTAGTTGACACAAATCA+AGG + chr7.1:32482234-32482253 MS.gene99484:CDS 30.0%
AATAGCAACATCAATATCCA+GGG + chr7.1:32483061-32483080 MS.gene99484:CDS 30.0%
ACACAAATCAAGGTACAATA+AGG + chr7.1:32482244-32482263 MS.gene99484:intron 30.0%
AGAAAACGAAAATGAAGATC+AGG - chr7.1:32481689-32481708 None:intergenic 30.0%
ATCTATGTGTTTGATCATCA+TGG - chr7.1:32481837-32481856 None:intergenic 30.0%
ATTAATTGAAACACTTGCCT+TGG - chr7.1:32481510-32481529 None:intergenic 30.0%
ATTCTTATCGAAAAACGAGA+AGG - chr7.1:32482683-32482702 None:intergenic 30.0%
GAAAGGAGTCAATATTTCTT+TGG + chr7.1:32483468-32483487 MS.gene99484:CDS 30.0%
GATCATCTCTTAAAGATTCA+TGG - chr7.1:32483180-32483199 None:intergenic 30.0%
GATGATTGTGAAATTAGTGA+AGG + chr7.1:32481533-32481552 MS.gene99484:CDS 30.0%
GTAACCTAGAATTTACAACA+TGG + chr7.1:32481805-32481824 MS.gene99484:intron 30.0%
TAATAGCAACATCAATATCC+AGG + chr7.1:32483060-32483079 MS.gene99484:CDS 30.0%
TGGTGATCATAAATGTATAG+AGG + chr7.1:32483345-32483364 MS.gene99484:CDS 30.0%
TTCAAATATGGATCTTGAGT+TGG + chr7.1:32483291-32483310 MS.gene99484:CDS 30.0%
TTCAATATCAAGTTAGTCCT+AGG + chr7.1:32481903-32481922 MS.gene99484:intron 30.0%
TTCTTATCGAAAAACGAGAA+GGG - chr7.1:32482682-32482701 None:intergenic 30.0%
TTGAATTGATTAACCTACCT+AGG - chr7.1:32481923-32481942 None:intergenic 30.0%
TTTACAGATGTGGATCATAT+GGG + chr7.1:32482942-32482961 MS.gene99484:intron 30.0%
! AAGGAAATTTGGAACAAAGT+TGG - chr7.1:32482814-32482833 None:intergenic 30.0%
! AGAAGAACAAGATAGTTCTA+AGG + chr7.1:32483438-32483457 MS.gene99484:CDS 30.0%
! AGTAATTTTACAACTGTCGT+TGG - chr7.1:32482887-32482906 None:intergenic 30.0%
! CGTTTAAAATTGTAACCTCA+AGG + chr7.1:32482844-32482863 MS.gene99484:intron 30.0%
! GATAATTTCCTCTCATTTTG+TGG + chr7.1:32483154-32483173 MS.gene99484:CDS 30.0%
! GATTTGAAGTTTAAACCTTG+AGG - chr7.1:32482862-32482881 None:intergenic 30.0%
! GGAAATTATCATTTGGTTCA+TGG - chr7.1:32483144-32483163 None:intergenic 30.0%
! TTGTACCATGTTTTCATACA+TGG - chr7.1:32482981-32483000 None:intergenic 30.0%
! TTTTACAGATGTGGATCATA+TGG + chr7.1:32482941-32482960 MS.gene99484:intron 30.0%
ATATCAAGTTAGTCCTAGGT+AGG + chr7.1:32481907-32481926 MS.gene99484:intron 35.0%
ATATGGATCAGAATGTTCCA+AGG + chr7.1:32481490-32481509 MS.gene99484:CDS 35.0%
ATGAGAGAGGAAGAAACATA+TGG + chr7.1:32481473-32481492 MS.gene99484:CDS 35.0%
CACACCATGTTGTAAATTCT+AGG - chr7.1:32481812-32481831 None:intergenic 35.0%
CTAGAATTTACAACATGGTG+TGG + chr7.1:32481810-32481829 MS.gene99484:intron 35.0%
CTTTCTTGCTTCTATACATC+TGG - chr7.1:32482218-32482237 None:intergenic 35.0%
GATGTGCAAAAGAACAACAA+TGG + chr7.1:32481563-32481582 MS.gene99484:CDS 35.0%
TCATGTGAAGAGTCATTTAC+AGG + chr7.1:32482078-32482097 MS.gene99484:CDS 35.0%
TCTTGCCATGTATGAAAACA+TGG + chr7.1:32482973-32482992 MS.gene99484:CDS 35.0%
TTACAGATGTGGATCATATG+GGG + chr7.1:32482943-32482962 MS.gene99484:intron 35.0%
TTTGGAAAATACTATGACCC+TGG - chr7.1:32483081-32483100 None:intergenic 35.0%
! AATTTTACAACTGTCGTTGG+AGG - chr7.1:32482884-32482903 None:intergenic 35.0%
! AGTTCTTCCTCTTGATTTTC+AGG - chr7.1:32483250-32483269 None:intergenic 35.0%
! GGGTCATAGTATTTTCCAAA+TGG + chr7.1:32483081-32483100 MS.gene99484:CDS 35.0%
! TAACTTGCCTGAAAATCAAG+AGG + chr7.1:32483240-32483259 MS.gene99484:CDS 35.0%
ATCTTTAACCATTACCCCTG+AGG + chr7.1:32482611-32482630 MS.gene99484:intron 40.0%
ATGTTAGATCCAAGTTGCCT+AGG + chr7.1:32481651-32481670 MS.gene99484:CDS 40.0%
ATTCATGGCCACAAAATGAG+AGG - chr7.1:32483165-32483184 None:intergenic 40.0%
CATAGGCTTCTTGTAGACAA+TGG + chr7.1:32482378-32482397 MS.gene99484:CDS 40.0%
GAAATGAGATCTTGTGCTCT+TGG - chr7.1:32483214-32483233 None:intergenic 40.0%
GGCAACTTGGATCTAACATA+AGG - chr7.1:32481650-32481669 None:intergenic 40.0%
GTGCAAAAGAACAACAATGG+AGG + chr7.1:32481566-32481585 MS.gene99484:CDS 40.0%
TCTTTAACCATTACCCCTGA+GGG + chr7.1:32482612-32482631 MS.gene99484:intron 40.0%
TGTAGGAGCAACTCCAAAAT+TGG + chr7.1:32482018-32482037 MS.gene99484:intron 40.0%
! GAATCTAGAGCTGAGTTCTA+TGG + chr7.1:32483016-32483035 MS.gene99484:CDS 40.0%
!! AGTTCTAAGGAGCAAGAGAA+AGG + chr7.1:32483451-32483470 MS.gene99484:CDS 40.0%
!! TAACAGTGTTGGCTGATCAT+AGG + chr7.1:32482361-32482380 MS.gene99484:intron 40.0%
AATGCTAACTAGTGCCCCAA+GGG + chr7.1:32482519-32482538 MS.gene99484:intron 45.0%
CTAACATAAGGCCTCACTGA+TGG - chr7.1:32481638-32481657 None:intergenic 45.0%
CTTAACCAATGCATGCCCTT+GGG - chr7.1:32482537-32482556 None:intergenic 45.0%
CTTCATGCTGTGCAAAGACT+TGG + chr7.1:32481707-32481726 MS.gene99484:CDS 45.0%
GTGAGAAGAAACCATCAGTG+AGG + chr7.1:32481624-32481643 MS.gene99484:CDS 45.0%
TTAACCAATGCATGCCCTTG+GGG - chr7.1:32482536-32482555 None:intergenic 45.0%
! ATGCTGTGCAAAGACTTGGT+GGG + chr7.1:32481711-32481730 MS.gene99484:CDS 45.0%
! GGAGTATAGCCTTGAAGCAT+GGG - chr7.1:32482435-32482454 None:intergenic 45.0%
! TGGAGTATAGCCTTGAAGCA+TGG - chr7.1:32482436-32482455 None:intergenic 45.0%
ACCCAACTTCCCATGCTTCA+AGG + chr7.1:32482423-32482442 MS.gene99484:CDS 50.0%
AGCCTTGAAGCATGGGAAGT+TGG - chr7.1:32482428-32482447 None:intergenic 50.0%
CATGCTGTGCAAAGACTTGG+TGG + chr7.1:32481710-32481729 MS.gene99484:CDS 50.0%
GAATGCTAACTAGTGCCCCA+AGG + chr7.1:32482518-32482537 MS.gene99484:intron 50.0%
GCCTTGAAGCATGGGAAGTT+GGG - chr7.1:32482427-32482446 None:intergenic 50.0%
GCTTAACCAATGCATGCCCT+TGG - chr7.1:32482538-32482557 None:intergenic 50.0%
CCAACGTAGCCTAGGCAACT+TGG - chr7.1:32481663-32481682 None:intergenic 55.0%
CCAAGTTGCCTAGGCTACGT+TGG + chr7.1:32481660-32481679 MS.gene99484:CDS 55.0%
CTTGTTAACCGGTGCCCTCA+GGG - chr7.1:32482629-32482648 None:intergenic 55.0%
TCAGGAGTCCAACGTAGCCT+AGG - chr7.1:32481671-32481690 None:intergenic 55.0%
TCTTGTTAACCGGTGCCCTC+AGG - chr7.1:32482630-32482649 None:intergenic 55.0%
TTGTTAACCGGTGCCCTCAG+GGG - chr7.1:32482628-32482647 None:intergenic 55.0%
! ACCATTACCCCTGAGGGCAC+CGG + chr7.1:32482618-32482637 MS.gene99484:intron 60.0%
! AGTGCCCCAAGGGCATGCAT+TGG + chr7.1:32482529-32482548 MS.gene99484:intron 60.0%
ACCGGTGCCCTCAGGGGTAA+TGG - chr7.1:32482622-32482641 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 32481467 32483504 32481467 ID=MS.gene99484
chr7.1 mRNA 32481467 32483504 32481467 ID=MS.gene99484.t1;Parent=MS.gene99484
chr7.1 exon 32481467 32481740 32481467 ID=MS.gene99484.t1.exon1;Parent=MS.gene99484.t1
chr7.1 CDS 32481467 32481740 32481467 ID=cds.MS.gene99484.t1;Parent=MS.gene99484.t1
chr7.1 exon 32482023 32482099 32482023 ID=MS.gene99484.t1.exon2;Parent=MS.gene99484.t1
chr7.1 CDS 32482023 32482099 32482023 ID=cds.MS.gene99484.t1;Parent=MS.gene99484.t1
chr7.1 exon 32482219 32482255 32482219 ID=MS.gene99484.t1.exon3;Parent=MS.gene99484.t1
chr7.1 CDS 32482219 32482255 32482219 ID=cds.MS.gene99484.t1;Parent=MS.gene99484.t1
chr7.1 exon 32482367 32482475 32482367 ID=MS.gene99484.t1.exon4;Parent=MS.gene99484.t1
chr7.1 CDS 32482367 32482475 32482367 ID=cds.MS.gene99484.t1;Parent=MS.gene99484.t1
chr7.1 exon 32482949 32483504 32482949 ID=MS.gene99484.t1.exon5;Parent=MS.gene99484.t1
chr7.1 CDS 32482949 32483504 32482949 ID=cds.MS.gene99484.t1;Parent=MS.gene99484.t1
Gene Sequence

>MS.gene99484

ATGAATATGAGAGAGGAAGAAACATATGGATCAGAATGTTCCAAGGCAAGTGTTTCAATTAATGAAGATGATTGTGAAATTAGTGAAGGAAATGAAGATGTGCAAAAGAACAACAATGGAGGAATAAGCTCAAGCAATAGTACTATAGAAGAGAATTGTGAGAAGAAACCATCAGTGAGGCCTTATGTTAGATCCAAGTTGCCTAGGCTACGTTGGACTCCTGATCTTCATTTTCGTTTTCTTCATGCTGTGCAAAGACTTGGTGGGCAAGAGAGTAAGTTCCACACACATAACATATATGTATATTTTTCTTATAATTATTAGCTAAGAATTGAAAAGTAACCTAGAATTTACAACATGGTGTGGACCATGATGATCAAACACATAGATATAGCTTCTTTTTTCCTTTTCTAGATTTCAATTTTTCATAGGGGATTTCAATATCAAGTTAGTCCTAGGTAGGTTAATCAATTCAACAATATGATTATTCTTGTTATTTTAATTTTAATTTTAATTTTAATTTTGTATGTATTGTTAATGATTTTCTTGTGTGTAGGAGCAACTCCAAAATTGGTTCTTCAATTGATGAATATAAAAGGTTTAAGTATTGCTCATGTGAAGAGTCATTTACAGGTAAAAAGTCATAGATAAATATGGTTGCAACCTATATATAGCTAGCTAGCTTTCAATATTTCTTAAGATTTATAATTGTTTAATTAATTTCTTTTCTTTCACATATATTTCTTTTCCAGATGTATAGAAGCAAGAAAGTAGTTGACACAAATCAAGGTACAATAAGGTTTTTCTTTTTTGTATGAATTCAACTATCAATAAGTGATTACATAATTAGGGTTTGTGCTTGCAAGTCACTAATTATTTATGATTGTATATATGTAACAGTGTTGGCTGATCATAGGCTTCTTGTAGACAATGGAGACAGAAATGTTTATAATCTCACCCAACTTCCCATGCTTCAAGGCTATACTCCAAATCAAACATCATCATATAGGTATACATATACTACTTGAATTAACCTTTTTTTTTTTATGGAGAATGCTAACTAGTGCCCCAAGGGCATGCATTGGTTAAGCATCTAATCAAAAGTAACATCTTATATTTCAAAGACAAAAATTCTATTTGTAGAATCTTTAACCATTACCCCTGAGGGCACCGGTTAACAAGACCTTTTTTTATTCATAGGGATCATATAAACCCTTCTCGTTTTTCGATAAGAATAAAGTTATCAAAACAAGTACAAAGGGTACTAAAATCGAGTTACCGAACTTGACCAAAAAATAAAATTAAAAAAATCGAGTTACACAAGAACAAAATACGAGTTGCTAACCAACTTTGTTCCAAATTTCCTTAAGTCATTTACGTTTAAAATTGTAACCTCAAGGTTTAAACTTCAAATCCTCCAACGACAGTTGTAAAATTACTGTTATTGTCTTAGAATTGAAGGTTTTTTTTTTTTTTTTACAGATGTGGATCATATGGGGATGCTTCTCTTGCCATGTATGAAAACATGGTACAAATGAGTTCAATAAGTGAATCTAGAGCTGAGTTCTATGGCAAAATGATTGAGAGAACCAATAATAGCAACATCAATATCCAGGGTCATAGTATTTTCCAAATGGATTCATCCAATTTTAGAGAACTATCAACAACTAAAGTCCATGAACCAAATGATAATTTCCTCTCATTTTGTGGCCATGAATCTTTAAGAGATGATCTACAAGTTCAGCCAAGAGCACAAGATCTCATTTCGAACGATAACTTGCCTGAAAATCAAGAGGAAGAACTGAAGACAATGAAAAGAAAAGCTTCAAATATGGATCTTGAGTTGGATCTATCACTAAAATTAAATTCAAGGAGCAATGGTGATCATAAATGTATAGAGGAGCATGAAATTGATAGCAATTTATCACTCTCTTTGTATTCTCAATCATCTTATGTTTGCAAAAGGATGAAAGAAGAACAAGATAGTTCTAAGGAGCAAGAGAAAGGAGTCAATATTTCTTTGGATCTAACCATATGA
Protein sequence

>MS.gene99484.t1

MNMREEETYGSECSKASVSINEDDCEISEGNEDVQKNNNGGISSSNSTIEENCEKKPSVRPYVRSKLPRLRWTPDLHFRFLHAVQRLGGQERATPKLVLQLMNIKGLSIAHVKSHLQMYRSKKVVDTNQVLADHRLLVDNGDRNVYNLTQLPMLQGYTPNQTSSYRCGSYGDASLAMYENMVQMSSISESRAEFYGKMIERTNNSNINIQGHSIFQMDSSNFRELSTTKVHEPNDNFLSFCGHESLRDDLQVQPRAQDLISNDNLPENQEEELKTMKRKASNMDLELDLSLKLNSRSNGDHKCIEEHEIDSNLSLSLYSQSSYVCKRMKEEQDSSKEQEKGVNISLDLTI