AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99498


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99498.t1 MTR_1g013130 33.333 174 89 7 26 179 27 193 9.70e-13 67.0
MS.gene99498.t1 MTR_3g011990 45.977 87 30 4 67 146 72 148 1.97e-11 63.5
MS.gene99498.t1 MTR_3g012010 49.254 67 27 1 66 125 71 137 2.31e-11 63.2
MS.gene99498.t1 MTR_5g077370 44.737 76 37 1 48 118 44 119 2.84e-11 62.4
MS.gene99498.t1 MTR_8g466760 46.667 75 27 3 77 148 86 150 3.85e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99498.t1 AT5G10970 39.837 123 48 6 63 179 84 186 1.32e-11 63.9
MS.gene99498.t1 AT5G25160 41.837 98 48 4 82 179 57 145 1.72e-11 63.2
MS.gene99498.t1 AT1G80730 68.293 41 11 1 78 118 60 98 5.38e-11 61.6

Find 81 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCAATGCCTTTCCTTCT+TGG 0.138422 7.1:+32240338 MS.gene99498:CDS
TTTGATTTCGACTCTAATTT+TGG 0.159297 7.1:-32239926 None:intergenic
TGAATTATGCCTTCTCTATT+AGG 0.184623 7.1:-32240613 None:intergenic
CCCCAAGCAACAACACAATT+TGG 0.187401 7.1:+32240448 MS.gene99498:CDS
TGGTGTTAGTGCCAAGTCTT+AGG 0.224597 7.1:-32240006 None:intergenic
TCTTGGCTCGGTTCACCTTA+TGG 0.231125 7.1:+32240355 MS.gene99498:CDS
CGGGTTGCAAAGGGTGTATT+TGG 0.256836 7.1:-32240400 None:intergenic
CCCAAATTGTGTTGTTGCTT+GGG 0.273537 7.1:-32240449 None:intergenic
GTTGTTGCTTGGGGTGAATT+TGG 0.275394 7.1:-32240439 None:intergenic
CCCAAAGTGTTTGACCTTTG+TGG 0.286115 7.1:-32240517 None:intergenic
GTTCACGCTTATGAGCATTT+TGG 0.317761 7.1:-32240177 None:intergenic
AGGTCAAACACTTTGGGTTT+GGG 0.338136 7.1:+32240523 MS.gene99498:CDS
CCACAAAGGTCAAACACTTT+GGG 0.340923 7.1:+32240517 MS.gene99498:CDS
GTGTTTGACCTTTGTGGAAT+TGG 0.343209 7.1:-32240511 None:intergenic
AAGGTCAAACACTTTGGGTT+TGG 0.344659 7.1:+32240522 MS.gene99498:CDS
CAAGGGTTTGGAAGTGGACT+TGG 0.360530 7.1:-32240026 None:intergenic
GTCACATATTGGACGCCAAT+TGG 0.375665 7.1:-32240319 None:intergenic
TCCCAAATTGTGTTGTTGCT+TGG 0.377648 7.1:-32240450 None:intergenic
TTCTCCACTTCTCAAGCTCT+TGG 0.384550 7.1:+32240148 MS.gene99498:CDS
TAGAAACCTCAAACGCAAAA+TGG 0.385914 7.1:+32239895 MS.gene99498:CDS
TCCACCGTTCTCCAAGGGTT+TGG 0.389944 7.1:-32240038 None:intergenic
CACCTTATGGTGGCTATGGA+GGG 0.398816 7.1:+32240368 MS.gene99498:CDS
GAAAGGCATTGGTCACATAT+TGG 0.406502 7.1:-32240330 None:intergenic
TAATAGAGAAGGCATAATTC+AGG 0.407241 7.1:+32240615 MS.gene99498:CDS
CCCAAGCAACAACACAATTT+GGG 0.410223 7.1:+32240449 MS.gene99498:CDS
TCCACAAAGGTCAAACACTT+TGG 0.411221 7.1:+32240516 MS.gene99498:CDS
TTAGGATCACGAGATAATAA+TGG 0.420098 7.1:-32240595 None:intergenic
ACCACTTTCTGCTCAGGTAA+AGG 0.442903 7.1:-32240094 None:intergenic
TCAAGCTCTATTCACTATCA+AGG 0.445678 7.1:+32240262 MS.gene99498:CDS
AATGCCTTTCCTTCTTGGCT+CGG 0.453136 7.1:+32240343 MS.gene99498:CDS
TTGGCATTTGCATCACATAC+CGG 0.471216 7.1:-32240420 None:intergenic
GTTCTCCAAGGGTTTGGAAG+TGG 0.473154 7.1:-32240032 None:intergenic
TCACATATTGGACGCCAATT+GGG 0.475144 7.1:-32240318 None:intergenic
TCACCTTATGGTGGCTATGG+AGG 0.475493 7.1:+32240367 MS.gene99498:CDS
TCCACTTCTCAAGCTCTTGG+AGG 0.477317 7.1:+32240151 MS.gene99498:CDS
GGTGTTAGTGCCAAGTCTTA+GGG 0.481355 7.1:-32240005 None:intergenic
GCTAACTTGCACAACCCAAT+TGG 0.486494 7.1:+32240304 MS.gene99498:CDS
AGTTGATGATGAGGAAACAT+AGG 0.490666 7.1:-32240644 None:intergenic
TAAAAGGAGAGAGGAAGAGA+TGG 0.501837 7.1:+32240222 MS.gene99498:CDS
GGTTCACCTTATGGTGGCTA+TGG 0.502207 7.1:+32240364 MS.gene99498:CDS
AACTCTACCACTTTCTGCTC+AGG 0.502340 7.1:-32240100 None:intergenic
CCAAATTGTGTTGTTGCTTG+GGG 0.502858 7.1:-32240448 None:intergenic
CAAGTCCACTTCCAAACCCT+TGG 0.519689 7.1:+32240027 MS.gene99498:CDS
TGATCAATGAAATTTCTTCG+AGG 0.520979 7.1:-32240553 None:intergenic
ATCACATACCGGGTTGCAAA+GGG 0.522044 7.1:-32240409 None:intergenic
ACCCCTCCATAGCCACCATA+AGG 0.522093 7.1:-32240370 None:intergenic
TGGCATTTGCATCACATACC+GGG 0.531856 7.1:-32240419 None:intergenic
TCTATCCTCATCATTTCCAA+TGG 0.532227 7.1:-32239876 None:intergenic
ACCTTATGGTGGCTATGGAG+GGG 0.537456 7.1:+32240369 MS.gene99498:CDS
ATTAGAGTCGAAATCAAAGA+AGG 0.542585 7.1:+32239931 MS.gene99498:CDS
CAAATACACCCTTTGCAACC+CGG 0.543155 7.1:+32240401 MS.gene99498:CDS
GTTGATGATGAGGAAACATA+GGG 0.545764 7.1:-32240643 None:intergenic
GAATAGAGCTTGAAAACCAT+AGG 0.547197 7.1:-32240252 None:intergenic
TCAACATCAGATTCAGAGGT+TGG 0.549087 7.1:-32239971 None:intergenic
TAGCACTACGGAAATATGAA+TGG 0.550571 7.1:-32240285 None:intergenic
AACCTCTGAATCTGATGTTG+AGG 0.555993 7.1:+32239973 MS.gene99498:CDS
ACCTCCAAGAGCTTGAGAAG+TGG 0.556669 7.1:-32240152 None:intergenic
AATGGAAGATAAAAGGAGAG+AGG 0.557828 7.1:+32240213 MS.gene99498:CDS
TGAATGTCCACCGTTCTCCA+AGG 0.561681 7.1:-32240044 None:intergenic
CATCACATACCGGGTTGCAA+AGG 0.564146 7.1:-32240410 None:intergenic
AAGAGATGGATTCAACCCTA+TGG 0.564603 7.1:+32240236 MS.gene99498:CDS
GAATGTCCACCGTTCTCCAA+GGG 0.565536 7.1:-32240043 None:intergenic
ACCTTTACCTGAGCAGAAAG+TGG 0.574184 7.1:+32240093 MS.gene99498:CDS
TAAGGTGAACCGAGCCAAGA+AGG 0.575815 7.1:-32240352 None:intergenic
ATTTCCAATGGAAAGTCTCT+CGG 0.582528 7.1:-32239864 None:intergenic
TGAACCGAGCCAAGAAGGAA+AGG 0.582959 7.1:-32240347 None:intergenic
GAGCCAAGAAGGAAAGGCAT+TGG 0.584408 7.1:-32240341 None:intergenic
ATGTCCGAGAGACTTTCCAT+TGG 0.589059 7.1:+32239860 MS.gene99498:CDS
AATAGAGCTTGAAAACCATA+GGG 0.593787 7.1:-32240251 None:intergenic
ATCCTCAACATCAGATTCAG+AGG 0.595365 7.1:-32239975 None:intergenic
TCTCGTGATCCTAATAGAGA+AGG 0.597683 7.1:+32240604 MS.gene99498:CDS
ACTTTCCATTGGAAATGATG+AGG 0.597982 7.1:+32239871 MS.gene99498:CDS
CCTCAAACGCAAAATGGTGG+AGG 0.605406 7.1:+32239901 MS.gene99498:CDS
GATTTACTAACCCTAAGACT+TGG 0.606458 7.1:+32239995 MS.gene99498:CDS
ACTTCCAAACCCTTGGAGAA+CGG 0.610472 7.1:+32240034 MS.gene99498:CDS
TGGCTCGGTTCACCTTATGG+TGG 0.610898 7.1:+32240358 MS.gene99498:CDS
AAACCTCAAACGCAAAATGG+TGG 0.616518 7.1:+32239898 MS.gene99498:CDS
GGTTGTGCAAGTTAGCACTA+CGG 0.648362 7.1:-32240297 None:intergenic
CGTTGATTGAGTTGATGATG+AGG 0.655857 7.1:-32240653 None:intergenic
TCCAAACCCTTGGAGAACGG+TGG 0.656704 7.1:+32240037 MS.gene99498:CDS
CTGCATTTCCAATTCCACAA+AGG 0.667747 7.1:+32240503 MS.gene99498:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAATGGAAGATAAA+AGG + chr7.1:32240206-32240225 MS.gene99498:CDS 15.0%
! TGAACGTGTCTTAAAAAAAA+TGG + chr7.1:32240195-32240214 MS.gene99498:CDS 25.0%
!!! AATTTGGGATGACTAATTTT+TGG + chr7.1:32240464-32240483 MS.gene99498:CDS 25.0%
!!! ATTTGGGATGACTAATTTTT+GGG + chr7.1:32240465-32240484 MS.gene99498:CDS 25.0%
!!! TTTGATTTCGACTCTAATTT+TGG - chr7.1:32239929-32239948 None:intergenic 25.0%
AATAGAGCTTGAAAACCATA+GGG - chr7.1:32240254-32240273 None:intergenic 30.0%
ATTAGAGTCGAAATCAAAGA+AGG + chr7.1:32239931-32239950 MS.gene99498:CDS 30.0%
TGAATTATGCCTTCTCTATT+AGG - chr7.1:32240616-32240635 None:intergenic 30.0%
TGATCAATGAAATTTCTTCG+AGG - chr7.1:32240556-32240575 None:intergenic 30.0%
TTAGGATCACGAGATAATAA+TGG - chr7.1:32240598-32240617 None:intergenic 30.0%
! TAATAGAGAAGGCATAATTC+AGG + chr7.1:32240615-32240634 MS.gene99498:CDS 30.0%
!! TTTGGGATGACTAATTTTTG+GGG + chr7.1:32240466-32240485 MS.gene99498:CDS 30.0%
AATGGAAGATAAAAGGAGAG+AGG + chr7.1:32240213-32240232 MS.gene99498:CDS 35.0%
ACTTTCCATTGGAAATGATG+AGG + chr7.1:32239871-32239890 MS.gene99498:CDS 35.0%
ATTTCCAATGGAAAGTCTCT+CGG - chr7.1:32239867-32239886 None:intergenic 35.0%
GAATAGAGCTTGAAAACCAT+AGG - chr7.1:32240255-32240274 None:intergenic 35.0%
GATTTACTAACCCTAAGACT+TGG + chr7.1:32239995-32240014 MS.gene99498:CDS 35.0%
TAGAAACCTCAAACGCAAAA+TGG + chr7.1:32239895-32239914 MS.gene99498:CDS 35.0%
TAGCACTACGGAAATATGAA+TGG - chr7.1:32240288-32240307 None:intergenic 35.0%
TCAAGCTCTATTCACTATCA+AGG + chr7.1:32240262-32240281 MS.gene99498:CDS 35.0%
TCTATCCTCATCATTTCCAA+TGG - chr7.1:32239879-32239898 None:intergenic 35.0%
!! AGTTGATGATGAGGAAACAT+AGG - chr7.1:32240647-32240666 None:intergenic 35.0%
!! GTTGATGATGAGGAAACATA+GGG - chr7.1:32240646-32240665 None:intergenic 35.0%
AAACCTCAAACGCAAAATGG+TGG + chr7.1:32239898-32239917 MS.gene99498:CDS 40.0%
AACCTCTGAATCTGATGTTG+AGG + chr7.1:32239973-32239992 MS.gene99498:CDS 40.0%
ATCCTCAACATCAGATTCAG+AGG - chr7.1:32239978-32239997 None:intergenic 40.0%
CCACAAAGGTCAAACACTTT+GGG + chr7.1:32240517-32240536 MS.gene99498:CDS 40.0%
CCCAAGCAACAACACAATTT+GGG + chr7.1:32240449-32240468 MS.gene99498:CDS 40.0%
CTGCATTTCCAATTCCACAA+AGG + chr7.1:32240503-32240522 MS.gene99498:CDS 40.0%
GTGTTTGACCTTTGTGGAAT+TGG - chr7.1:32240514-32240533 None:intergenic 40.0%
TAAAAGGAGAGAGGAAGAGA+TGG + chr7.1:32240222-32240241 MS.gene99498:CDS 40.0%
TCAACATCAGATTCAGAGGT+TGG - chr7.1:32239974-32239993 None:intergenic 40.0%
TCACATATTGGACGCCAATT+GGG - chr7.1:32240321-32240340 None:intergenic 40.0%
TCCACAAAGGTCAAACACTT+TGG + chr7.1:32240516-32240535 MS.gene99498:CDS 40.0%
TCTCGTGATCCTAATAGAGA+AGG + chr7.1:32240604-32240623 MS.gene99498:CDS 40.0%
TTGGCATTTGCATCACATAC+CGG - chr7.1:32240423-32240442 None:intergenic 40.0%
! AAGAGATGGATTCAACCCTA+TGG + chr7.1:32240236-32240255 MS.gene99498:CDS 40.0%
! AAGGTCAAACACTTTGGGTT+TGG + chr7.1:32240522-32240541 MS.gene99498:CDS 40.0%
! AGGTCAAACACTTTGGGTTT+GGG + chr7.1:32240523-32240542 MS.gene99498:CDS 40.0%
! CCAAATTGTGTTGTTGCTTG+GGG - chr7.1:32240451-32240470 None:intergenic 40.0%
! CCCAAATTGTGTTGTTGCTT+GGG - chr7.1:32240452-32240471 None:intergenic 40.0%
! GTTCACGCTTATGAGCATTT+TGG - chr7.1:32240180-32240199 None:intergenic 40.0%
! TCCCAAATTGTGTTGTTGCT+TGG - chr7.1:32240453-32240472 None:intergenic 40.0%
!! CGTTGATTGAGTTGATGATG+AGG - chr7.1:32240656-32240675 None:intergenic 40.0%
!! GAAAGGCATTGGTCACATAT+TGG - chr7.1:32240333-32240352 None:intergenic 40.0%
!!! ATGACTAATTTTTGGGGTGG+AGG + chr7.1:32240472-32240491 MS.gene99498:CDS 40.0%
AACTCTACCACTTTCTGCTC+AGG - chr7.1:32240103-32240122 None:intergenic 45.0%
AATGCCTTTCCTTCTTGGCT+CGG + chr7.1:32240343-32240362 MS.gene99498:CDS 45.0%
ACCACTTTCTGCTCAGGTAA+AGG - chr7.1:32240097-32240116 None:intergenic 45.0%
ACCTTTACCTGAGCAGAAAG+TGG + chr7.1:32240093-32240112 MS.gene99498:CDS 45.0%
ACTTCCAAACCCTTGGAGAA+CGG + chr7.1:32240034-32240053 MS.gene99498:CDS 45.0%
ATCACATACCGGGTTGCAAA+GGG - chr7.1:32240412-32240431 None:intergenic 45.0%
ATGTCCGAGAGACTTTCCAT+TGG + chr7.1:32239860-32239879 MS.gene99498:CDS 45.0%
CAAATACACCCTTTGCAACC+CGG + chr7.1:32240401-32240420 MS.gene99498:CDS 45.0%
CCCCAAGCAACAACACAATT+TGG + chr7.1:32240448-32240467 MS.gene99498:CDS 45.0%
GGTGTTAGTGCCAAGTCTTA+GGG - chr7.1:32240008-32240027 None:intergenic 45.0%
GGTTGTGCAAGTTAGCACTA+CGG - chr7.1:32240300-32240319 None:intergenic 45.0%
GTCACATATTGGACGCCAAT+TGG - chr7.1:32240322-32240341 None:intergenic 45.0%
TGACCAATGCCTTTCCTTCT+TGG + chr7.1:32240338-32240357 MS.gene99498:CDS 45.0%
TGGCATTTGCATCACATACC+GGG - chr7.1:32240422-32240441 None:intergenic 45.0%
TTCTCCACTTCTCAAGCTCT+TGG + chr7.1:32240148-32240167 MS.gene99498:CDS 45.0%
! CCCAAAGTGTTTGACCTTTG+TGG - chr7.1:32240520-32240539 None:intergenic 45.0%
! GCTAACTTGCACAACCCAAT+TGG + chr7.1:32240304-32240323 MS.gene99498:CDS 45.0%
! GTTGTTGCTTGGGGTGAATT+TGG - chr7.1:32240442-32240461 None:intergenic 45.0%
! TGGTGTTAGTGCCAAGTCTT+AGG - chr7.1:32240009-32240028 None:intergenic 45.0%
!! GGGATGACTAATTTTTGGGG+TGG + chr7.1:32240469-32240488 MS.gene99498:CDS 45.0%
ACCTCCAAGAGCTTGAGAAG+TGG - chr7.1:32240155-32240174 None:intergenic 50.0%
ACCTTATGGTGGCTATGGAG+GGG + chr7.1:32240369-32240388 MS.gene99498:CDS 50.0%
CAAGTCCACTTCCAAACCCT+TGG + chr7.1:32240027-32240046 MS.gene99498:CDS 50.0%
CACCTTATGGTGGCTATGGA+GGG + chr7.1:32240368-32240387 MS.gene99498:CDS 50.0%
CATCACATACCGGGTTGCAA+AGG - chr7.1:32240413-32240432 None:intergenic 50.0%
CCTCAAACGCAAAATGGTGG+AGG + chr7.1:32239901-32239920 MS.gene99498:CDS 50.0%
GAATGTCCACCGTTCTCCAA+GGG - chr7.1:32240046-32240065 None:intergenic 50.0%
GGTTCACCTTATGGTGGCTA+TGG + chr7.1:32240364-32240383 MS.gene99498:CDS 50.0%
GTTCTCCAAGGGTTTGGAAG+TGG - chr7.1:32240035-32240054 None:intergenic 50.0%
TAAGGTGAACCGAGCCAAGA+AGG - chr7.1:32240355-32240374 None:intergenic 50.0%
TCACCTTATGGTGGCTATGG+AGG + chr7.1:32240367-32240386 MS.gene99498:CDS 50.0%
TCCACTTCTCAAGCTCTTGG+AGG + chr7.1:32240151-32240170 MS.gene99498:CDS 50.0%
TGAACCGAGCCAAGAAGGAA+AGG - chr7.1:32240350-32240369 None:intergenic 50.0%
TGAATGTCCACCGTTCTCCA+AGG - chr7.1:32240047-32240066 None:intergenic 50.0%
! CAAGGGTTTGGAAGTGGACT+TGG - chr7.1:32240029-32240048 None:intergenic 50.0%
! CCTCCACCATTTTGCGTTTG+AGG - chr7.1:32239904-32239923 None:intergenic 50.0%
! CGGGTTGCAAAGGGTGTATT+TGG - chr7.1:32240403-32240422 None:intergenic 50.0%
! GAGCCAAGAAGGAAAGGCAT+TGG - chr7.1:32240344-32240363 None:intergenic 50.0%
!! TCTTGGCTCGGTTCACCTTA+TGG + chr7.1:32240355-32240374 MS.gene99498:CDS 50.0%
ACCCCTCCATAGCCACCATA+AGG - chr7.1:32240373-32240392 None:intergenic 55.0%
TCCAAACCCTTGGAGAACGG+TGG + chr7.1:32240037-32240056 MS.gene99498:CDS 55.0%
TCCACCGTTCTCCAAGGGTT+TGG - chr7.1:32240041-32240060 None:intergenic 55.0%
!! TGGCTCGGTTCACCTTATGG+TGG + chr7.1:32240358-32240377 MS.gene99498:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 32239860 32240678 32239860 ID=MS.gene99498
chr7.1 mRNA 32239860 32240678 32239860 ID=MS.gene99498.t1;Parent=MS.gene99498
chr7.1 exon 32239860 32240678 32239860 ID=MS.gene99498.t1.exon1;Parent=MS.gene99498.t1
chr7.1 CDS 32239860 32240678 32239860 ID=cds.MS.gene99498.t1;Parent=MS.gene99498.t1
Gene Sequence

>MS.gene99498

ATGTCCGAGAGACTTTCCATTGGAAATGATGAGGATAGAAACCTCAAACGCAAAATGGTGGAGGAGCCAAAATTAGAGTCGAAATCAAAGAAGGAGTCAAAGACAATGACACCAACCTCTGAATCTGATGTTGAGGATTTACTAACCCTAAGACTTGGCACTAACACCAAGTCCACTTCCAAACCCTTGGAGAACGGTGGACATTCAAGTTCATTTCAAAACACTACTGATGAACCTTTACCTGAGCAGAAAGTGGTAGAGTTCTCTTGTAATTATTGTGACAAGAAATTCTCCACTTCTCAAGCTCTTGGAGGTCACCAAAATGCTCATAAGCGTGAACGTGTCTTAAAAAAAATGGAAGATAAAAGGAGAGAGGAAGAGATGGATTCAACCCTATGGTTTTCAAGCTCTATTCACTATCAAGGCCATTCATATTTCCGTAGTGCTAACTTGCACAACCCAATTGGCGTCCAATATGTGACCAATGCCTTTCCTTCTTGGCTCGGTTCACCTTATGGTGGCTATGGAGGGGTGTATATGCCAAATACACCCTTTGCAACCCGGTATGTGATGCAAATGCCAAATTCACCCCAAGCAACAACACAATTTGGGATGACTAATTTTTGGGGTGGAGGTCAAAATGCTGCATTTCCAATTCCACAAAGGTCAAACACTTTGGGTTTGGGACTTTTTCCTCGAAGAAATTTCATTGATCAACTTCGTTCTCATACTCTTCCATTATTATCTCGTGATCCTAATAGAGAAGGCATAATTCAGGCAAATCCCTATGTTTCCTCATCATCAACTCAATCAACGTAG

Protein sequence

>MS.gene99498.t1

MSERLSIGNDEDRNLKRKMVEEPKLESKSKKESKTMTPTSESDVEDLLTLRLGTNTKSTSKPLENGGHSSSFQNTTDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDKRREEEMDSTLWFSSSIHYQGHSYFRSANLHNPIGVQYVTNAFPSWLGSPYGGYGGVYMPNTPFATRYVMQMPNSPQATTQFGMTNFWGGGQNAAFPIPQRSNTLGLGLFPRRNFIDQLRSHTLPLLSRDPNREGIIQANPYVSSSSTQST