AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99585

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99585.t1 MTR_8g072590 77.673 636 81 7 5 582 26 658 0.0 978
MS.gene99585.t1 MTR_8g073190 48.433 702 200 20 15 572 49 732 0.0 564
MS.gene99585.t1 MTR_5g024510 36.016 733 286 20 15 572 65 789 2.18e-117 372
MS.gene99585.t1 MTR_5g024510 35.000 700 272 20 15 539 65 756 2.56e-104 334
MS.gene99585.t1 MTR_5g024510 34.880 668 256 18 79 572 5 667 4.05e-97 315
MS.gene99585.t1 MTR_4g086660 28.931 318 161 17 292 553 93 401 3.18e-16 82.8
MS.gene99585.t1 MTR_8g024590 27.881 269 159 11 318 560 20 279 1.16e-14 77.0
MS.gene99585.t1 MTR_1g101630 31.325 249 129 12 339 553 61 301 1.83e-14 77.0
MS.gene99585.t1 MTR_8g024600 27.509 269 160 11 318 560 20 279 3.65e-14 75.5
MS.gene99585.t1 MTR_7g118020 27.778 270 147 11 329 556 148 411 5.99e-14 75.5
MS.gene99585.t1 MTR_5g092815 25.974 308 171 13 301 567 67 358 6.62e-14 74.3
MS.gene99585.t1 MTR_5g017830 26.567 335 182 16 266 553 81 398 1.38e-13 74.3
MS.gene99585.t1 MTR_4g114670 25.651 269 177 7 306 554 2 267 3.17e-13 72.4
MS.gene99585.t1 MTR_4g114670 25.651 269 177 7 306 554 2 267 3.85e-13 72.4
MS.gene99585.t1 MTR_2g016340 26.259 278 155 13 322 558 5 273 4.26e-13 72.4
MS.gene99585.t1 MTR_5g017890 28.444 225 122 9 361 555 422 637 4.49e-13 72.8
MS.gene99585.t1 MTR_0028s0170 29.665 209 110 10 372 552 138 337 5.68e-13 72.4
MS.gene99585.t1 MTR_8g079790 28.115 313 160 15 309 563 96 401 7.15e-13 72.0
MS.gene99585.t1 MTR_2g020380 27.481 262 153 9 331 560 10 266 7.76e-13 72.4
MS.gene99585.t1 MTR_3g101210 28.959 221 122 9 361 553 357 570 7.84e-13 72.0
MS.gene99585.t1 MTR_3g101210 28.959 221 122 9 361 553 357 570 7.90e-13 72.0
MS.gene99585.t1 MTR_5g089320 29.464 224 121 10 370 565 126 340 1.11e-12 71.2
MS.gene99585.t1 MTR_4g019410 27.719 285 162 14 310 557 5 282 1.12e-12 71.2
MS.gene99585.t1 MTR_4g019410 28.364 275 153 14 320 557 15 282 2.60e-12 70.5
MS.gene99585.t1 MTR_5g022030 26.616 263 149 13 339 565 119 373 3.34e-12 69.7
MS.gene99585.t1 MTR_4g082325 27.413 259 150 11 325 547 143 399 3.37e-12 69.3
MS.gene99585.t1 MTR_5g022030 26.616 263 149 13 339 565 119 373 5.00e-12 69.3
MS.gene99585.t1 MTR_0007s0020 25.344 363 194 17 258 548 488 845 7.00e-12 69.3
MS.gene99585.t1 MTR_4g132070 27.181 298 164 14 301 552 60 350 8.71e-12 68.6
MS.gene99585.t1 MTR_5g075100 27.935 247 147 9 341 560 45 287 1.53e-11 67.4
MS.gene99585.t1 MTR_1g105290 26.966 267 154 11 318 550 58 317 1.71e-11 67.8
MS.gene99585.t1 MTR_1g026190 25.333 300 167 14 324 581 77 361 1.77e-11 67.4
MS.gene99585.t1 MTR_1g100250 26.484 219 131 9 359 554 459 670 1.82e-11 67.8
MS.gene99585.t1 MTR_1g105290 26.966 267 154 11 318 550 58 317 1.85e-11 67.8
MS.gene99585.t1 MTR_8g099095 29.858 211 111 10 370 552 124 325 1.90e-11 67.4
MS.gene99585.t1 MTR_1g105290 26.866 268 155 11 318 551 58 318 2.12e-11 67.0
MS.gene99585.t1 MTR_8g024100 28.358 268 155 12 319 552 10 274 2.27e-11 67.4
MS.gene99585.t1 MTR_5g092810 26.619 278 148 14 319 552 86 351 4.59e-11 66.2
MS.gene99585.t1 MTR_4g094415 28.302 212 130 6 360 553 212 419 7.61e-11 65.5
MS.gene99585.t1 MTR_5g088350 27.632 304 170 14 318 586 16 304 8.28e-11 64.3
MS.gene99585.t1 MTR_3g098070 28.832 274 143 16 319 550 23 286 8.31e-11 64.3
MS.gene99585.t1 MTR_7g113490 27.419 248 114 8 346 550 106 330 8.31e-11 65.5
MS.gene99585.t1 MTR_3g096230 26.106 226 144 8 361 566 294 516 8.43e-11 65.5
MS.gene99585.t1 MTR_5g088350 27.632 304 170 14 318 586 16 304 8.98e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99585.t1 AT2G17520 45.794 321 108 7 315 570 404 723 1.32e-78 267
MS.gene99585.t1 AT5G24360 45.283 318 113 6 313 570 447 763 1.69e-76 262
MS.gene99585.t1 AT5G24360 45.283 318 113 6 313 570 453 769 2.20e-76 262
MS.gene99585.t1 AT5G24360 45.283 318 113 6 313 570 433 749 2.33e-76 261
MS.gene99585.t1 AT3G11870 36.164 318 135 13 318 570 113 427 5.12e-40 154
MS.gene99585.t1 AT5G66850 27.308 260 152 11 361 592 396 646 2.82e-15 80.1
MS.gene99585.t1 AT5G66850 27.308 260 152 11 361 592 396 646 4.12e-15 79.3
MS.gene99585.t1 AT2G41140 26.421 299 171 12 300 556 99 390 1.25e-14 77.8
MS.gene99585.t1 AT3G56760 26.756 299 170 12 300 556 100 391 2.97e-14 76.6
MS.gene99585.t1 AT1G53570 27.602 221 138 7 361 562 264 481 1.27e-13 74.7
MS.gene99585.t1 AT1G53570 27.602 221 138 7 361 562 264 481 1.27e-13 74.3
MS.gene99585.t1 AT1G53570 27.602 221 138 7 361 562 264 481 1.29e-13 74.7
MS.gene99585.t1 AT1G53570 27.602 221 138 7 361 562 264 481 1.29e-13 74.7
MS.gene99585.t1 AT1G53570 27.602 221 138 7 361 562 264 481 1.33e-13 74.3
MS.gene99585.t1 AT1G53570 27.602 221 138 7 361 562 264 481 1.33e-13 74.3
MS.gene99585.t1 AT5G24430 26.786 280 153 12 329 565 147 417 2.49e-13 73.6
MS.gene99585.t1 AT3G20410 27.863 262 145 11 329 552 95 350 3.37e-13 73.2
MS.gene99585.t1 AT3G20410 27.863 262 145 11 329 552 95 350 3.37e-13 73.2
MS.gene99585.t1 AT2G46700 25.667 300 174 12 300 556 117 410 3.79e-13 73.2
MS.gene99585.t1 AT2G37840 29.348 276 149 13 318 555 6 273 6.65e-13 72.4
MS.gene99585.t1 AT2G30360 29.183 257 137 11 339 560 41 287 1.63e-12 70.5
MS.gene99585.t1 AT4G04710 27.530 247 149 9 330 553 41 280 1.71e-12 70.5
MS.gene99585.t1 AT4G04710 27.948 229 139 7 348 553 69 294 1.91e-12 70.5
MS.gene99585.t1 AT3G49370 28.794 257 140 11 343 565 169 416 1.94e-12 70.9
MS.gene99585.t1 AT3G48750 28.049 246 127 10 356 553 46 289 2.73e-12 68.6
MS.gene99585.t1 AT3G10540 26.022 269 162 10 318 552 48 313 3.44e-12 69.7
MS.gene99585.t1 AT1G12680 24.111 253 162 10 325 551 107 355 4.47e-12 69.3
MS.gene99585.t1 AT5G04510 24.254 268 166 9 318 551 47 311 5.04e-12 69.3
MS.gene99585.t1 AT5G57630 32.020 203 114 9 318 501 15 212 5.45e-12 68.6
MS.gene99585.t1 AT1G54960 24.731 279 175 10 345 598 105 373 5.79e-12 69.3
MS.gene99585.t1 AT1G54960 24.731 279 175 10 345 598 105 373 5.79e-12 69.3
MS.gene99585.t1 AT3G45240 25.237 317 180 11 286 559 72 374 6.78e-12 68.2
MS.gene99585.t1 AT5G57630 32.020 203 114 9 318 501 15 212 7.15e-12 68.2
MS.gene99585.t1 AT5G04510 24.254 268 166 9 318 551 47 311 7.41e-12 68.2
MS.gene99585.t1 AT1G49580 28.947 266 141 12 329 552 154 413 1.03e-11 68.6
MS.gene99585.t1 AT1G12580 28.502 207 117 7 370 551 102 302 1.16e-11 68.2
MS.gene99585.t1 AT5G21326 27.778 306 165 14 326 586 10 304 1.35e-11 67.8
MS.gene99585.t1 AT3G45240 25.000 320 180 11 286 559 72 377 1.44e-11 67.4
MS.gene99585.t1 AT3G45240 25.000 320 180 11 286 559 72 377 1.44e-11 67.4
MS.gene99585.t1 AT1G50700 29.327 208 116 8 370 552 131 332 1.81e-11 67.4
MS.gene99585.t1 AT1G50700 29.327 208 116 8 370 552 131 332 1.89e-11 67.4
MS.gene99585.t1 AT4G12020 31.100 209 116 9 362 550 1601 1801 1.93e-11 68.2
MS.gene99585.t1 AT4G12020 31.100 209 116 9 362 550 1668 1868 1.94e-11 68.2
MS.gene99585.t1 AT4G12020 31.100 209 116 9 362 550 1676 1876 1.98e-11 68.2
MS.gene99585.t1 AT1G50700 26.592 267 152 11 324 552 72 332 2.04e-11 67.4
MS.gene99585.t1 AT1G09000 25.403 248 132 9 346 557 107 337 2.19e-11 67.4
MS.gene99585.t1 AT3G61960 29.555 247 142 12 339 556 30 273 2.29e-11 67.4
MS.gene99585.t1 AT3G61960 29.675 246 143 11 339 556 30 273 2.32e-11 67.4
MS.gene99585.t1 AT4G30960 27.354 223 128 9 360 557 71 284 2.43e-11 66.6
MS.gene99585.t1 AT1G09000 25.506 247 131 9 346 556 107 336 2.49e-11 67.4
MS.gene99585.t1 AT2G26980 28.053 303 168 15 318 585 17 304 2.62e-11 66.2
MS.gene99585.t1 AT4G04695 26.838 272 161 11 321 555 24 294 2.95e-11 66.6
MS.gene99585.t1 AT3G53930 26.829 287 164 12 307 555 3 281 3.57e-11 67.0
MS.gene99585.t1 AT2G26980 28.053 303 168 15 318 585 17 304 3.67e-11 66.2
MS.gene99585.t1 AT3G53930 26.829 287 164 12 307 555 3 281 3.80e-11 66.6
MS.gene99585.t1 AT2G34180 23.939 330 197 12 270 563 12 323 3.99e-11 66.2
MS.gene99585.t1 AT2G26980 28.053 303 168 15 318 585 27 314 4.03e-11 66.2
MS.gene99585.t1 AT2G26980 28.053 303 168 15 318 585 17 304 4.45e-11 65.9
MS.gene99585.t1 AT4G04700 26.087 276 158 13 321 555 24 294 5.04e-11 65.9
MS.gene99585.t1 AT1G14000 27.083 240 141 10 340 547 181 418 9.32e-11 65.1

Find 133 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACGTCGGGATCAGGATTT+TGG 0.142257 8.3:-29797088 MS.gene99585:CDS
CAACTTAGGGGTATTGTTGC+AGG 0.255348 8.3:-29796090 MS.gene99585:intron
TTGTTGAAAAGGAAAATATT+AGG 0.257697 8.3:-29794312 MS.gene99585:CDS
TCAATTCCTTGATGCTGCTC+TGG 0.258221 8.3:+29795550 None:intergenic
CTCTGCATATCATGAGTTAA+AGG 0.258755 8.3:+29794208 None:intergenic
CAATGATCATGATGGTAATA+TGG 0.262900 8.3:-29796807 MS.gene99585:CDS
TATAATGGAGAAGCTGTAAT+TGG 0.296066 8.3:-29797262 MS.gene99585:CDS
TGATGTTCCTTGACCACAAT+TGG 0.301365 8.3:+29794337 None:intergenic
CGCTGCTGATCAATATTTAT+AGG 0.306460 8.3:-29797139 MS.gene99585:CDS
CAATTCCTTGATGCTGCTCT+GGG 0.308125 8.3:+29795551 None:intergenic
CATTTGGAGATGATCATCTT+TGG 0.309328 8.3:-29795459 MS.gene99585:CDS
TACTTGTAACTTAAATGATT+TGG 0.312272 8.3:-29796294 MS.gene99585:CDS
CAATGATTTACCTTAGATTC+TGG 0.314537 8.3:+29795341 None:intergenic
TTCGTCAAGTATGGTGTAAA+AGG 0.320237 8.3:+29794169 None:intergenic
AAACTCTCTTCTGGGAATTC+CGG 0.326031 8.3:+29794404 None:intergenic
CCTATCATAATTTCATATAA+TGG 0.329824 8.3:-29797277 MS.gene99585:CDS
CCAACCTGTACAGTAGGATT+TGG 0.344486 8.3:+29795579 None:intergenic
CCATTATATGAAATTATGAT+AGG 0.350325 8.3:+29797277 None:intergenic
GCTGCAGACATATTCAGTTT+AGG 0.353862 8.3:-29795520 MS.gene99585:CDS
TGTTGAAAAGGAAAATATTA+GGG 0.360331 8.3:-29794311 MS.gene99585:CDS
TCGAAAAGATCTTTCCTTAG+TGG 0.367434 8.3:-29795413 MS.gene99585:CDS
ACCTTCCATTCTTCCTTCCC+TGG 0.372121 8.3:+29796477 None:intergenic
GTAGATGGCCACAAAATAAA+TGG 0.373510 8.3:+29797721 None:intergenic
TAAACTCTGACCAGAATCTA+AGG 0.375847 8.3:-29795351 MS.gene99585:intron
AGAAGCCGCTTGACTGCAAC+TGG 0.379342 8.3:+29796443 None:intergenic
ACTTCTCTATTATTATCCTT+TGG 0.382863 8.3:+29796572 None:intergenic
TACTGCTATCTCGTGGTAAA+GGG 0.383720 8.3:-29796614 MS.gene99585:CDS
GGCATTAACCAACATGTTCC+TGG 0.384010 8.3:-29795964 MS.gene99585:intron
TTCCTTAGTGGAGTTCATTC+CGG 0.386781 8.3:-29795401 MS.gene99585:CDS
CATGGGACCATTCTCGTAGA+TGG 0.392921 8.3:+29797706 None:intergenic
TTGCGTGTCCACAACATCAT+TGG 0.395607 8.3:+29794279 None:intergenic
CTCATGATATGCAGAGCAAT+GGG 0.397313 8.3:-29794201 MS.gene99585:CDS
CTAATAAGATCCTCAGCTTC+CGG 0.400149 8.3:+29795382 None:intergenic
AACATTAATGAAGGTGTTGA+TGG 0.406190 8.3:-29796542 MS.gene99585:CDS
GGAACATCAGATTGTTGAAA+AGG 0.408020 8.3:-29794323 MS.gene99585:CDS
CTACTGCTATCTCGTGGTAA+AGG 0.421446 8.3:-29796615 MS.gene99585:CDS
ACTAAGGGAAGCCATCCATT+TGG 0.421454 8.3:-29795475 MS.gene99585:CDS
GATGGTCCCACGTCGGGATC+AGG 0.425123 8.3:-29797095 MS.gene99585:CDS
CGTTGAGGTTTCACCTGATC+AGG 0.426933 8.3:-29794254 MS.gene99585:CDS
CCATGCCATTTGACAATGTT+CGG 0.427560 8.3:+29796362 None:intergenic
ATTGGCCAGGGAAGGAAGAA+TGG 0.434234 8.3:-29796482 MS.gene99585:CDS
GTCAGAAAGCTTTACACATA+AGG 0.445083 8.3:+29795990 None:intergenic
AGTTCACATGGTGCTTATCA+TGG 0.451030 8.3:-29796905 MS.gene99585:CDS
AAACTGTTTGCCGCACCTGA+TGG 0.455756 8.3:-29797746 MS.gene99585:CDS
CTTAAATGATTTGGTTCAAG+TGG 0.456881 8.3:-29796285 MS.gene99585:CDS
AACTGTTTGCCGCACCTGAT+GGG 0.457845 8.3:-29797745 MS.gene99585:CDS
ACCTGCTCTTGTACCTGATC+AGG 0.458556 8.3:+29794241 None:intergenic
AACTTAGGGGTATTGTTGCA+GGG 0.461822 8.3:-29796089 MS.gene99585:intron
TTCGCCAAATCCTACTGTAC+AGG 0.462671 8.3:-29795583 MS.gene99585:CDS
GCTTGATACGAGCTGTAGAT+TGG 0.462728 8.3:+29797653 None:intergenic
TAGTATGTTCGTTATTCTGT+TGG 0.467334 8.3:-29796651 MS.gene99585:CDS
AAAGGAAAATATTAGGGTTG+AGG 0.475152 8.3:-29794305 MS.gene99585:CDS
GGTGTTGATGGAGAAATAAT+TGG 0.476448 8.3:-29796530 MS.gene99585:CDS
AAGTATGATGGTCCCACGTC+GGG 0.481203 8.3:-29797101 MS.gene99585:CDS
TTTGTGGCCATCTACGAGAA+TGG 0.482936 8.3:-29797713 MS.gene99585:CDS
GGATAAGTACTACGGCAAAC+TGG 0.485253 8.3:-29797576 MS.gene99585:intron
ACAAAATAAATGGTCCCATC+AGG 0.485253 8.3:+29797731 None:intergenic
GATGTTCCTTGACCACAATT+GGG 0.486905 8.3:+29794338 None:intergenic
AATTAGTTCCGTCTTGTCAC+TGG 0.487641 8.3:+29794366 None:intergenic
TCAAAATGTTCTAATAATCA+AGG 0.488409 8.3:-29796019 MS.gene99585:CDS
AAAAGATGAAAGTTCTCCCC+TGG 0.489626 8.3:-29798018 None:intergenic
ACCTGATCAGGTACAAGAGC+AGG 0.499859 8.3:-29794242 MS.gene99585:CDS
ATTTGACAATGTTCGGATGA+TGG 0.500705 8.3:+29796369 None:intergenic
AGGAAGAATGGAAGGTATGA+TGG 0.501897 8.3:-29796470 MS.gene99585:CDS
TTTGATGCATTTAGATTCCC+AGG 0.505866 8.3:+29797164 None:intergenic
CTCACTGAGAGTGATTTACT+AGG 0.506869 8.3:-29796719 MS.gene99585:CDS
TGCTTCTTTAGACATGGGTA+AGG 0.508181 8.3:+29796981 None:intergenic
TGCAACACATCAATTGCGTT+CGG 0.511163 8.3:+29794439 None:intergenic
ATGGTCCCATGAGGATATTA+TGG 0.514333 8.3:-29797694 MS.gene99585:CDS
ATCATCCGAACATTGTCAAA+TGG 0.518090 8.3:-29796367 MS.gene99585:CDS
CAAATCCTACTGTACAGGTT+GGG 0.523128 8.3:-29795578 MS.gene99585:CDS
TACAGCTCGTATCAAGCTCC+CGG 0.524482 8.3:-29797647 MS.gene99585:CDS
CCAAATCCTACTGTACAGGT+TGG 0.530465 8.3:-29795579 MS.gene99585:CDS
GCTGATCAATATTTATAGGA+AGG 0.534128 8.3:-29797135 MS.gene99585:CDS
GTTGTTCATTTGCATAAACT+AGG 0.535949 8.3:-29796066 MS.gene99585:CDS
ATGTGTAAAGCTTTCTGACA+TGG 0.538576 8.3:-29795986 MS.gene99585:CDS
GAATGACCATAATATCCTCA+TGG 0.539079 8.3:+29797688 None:intergenic
CCGAACATTGTCAAATGGCA+TGG 0.540381 8.3:-29796362 MS.gene99585:CDS
TGATGACCCAATTGTGGTCA+AGG 0.540711 8.3:-29794344 MS.gene99585:CDS
ATTTGAACAAGAAGAGTGGA+TGG 0.546110 8.3:-29796688 MS.gene99585:CDS
AACAGAATAACGAACATACT+AGG 0.547666 8.3:+29796653 None:intergenic
TACAACAAAATAATTACAAG+AGG 0.548877 8.3:-29796763 MS.gene99585:CDS
TGTTGTAGAAAAGGCGTAGG+AGG 0.551767 8.3:+29796779 None:intergenic
TGAATCATCATATCTGAGGC+AGG 0.554368 8.3:+29796857 None:intergenic
TGTGTAAAGCTTTCTGACAT+GGG 0.554737 8.3:-29795985 MS.gene99585:CDS
TGGGACCATCATACTTCAGA+TGG 0.558649 8.3:+29797108 None:intergenic
TTCCGGAATGAACTCCACTA+AGG 0.560655 8.3:+29795399 None:intergenic
CAAAGATGATCATCTCCAAA+TGG 0.562429 8.3:+29795460 None:intergenic
ACTTAGGGGTATTGTTGCAG+GGG 0.563653 8.3:-29796088 MS.gene99585:intron
CATGAAATGTAGTATGATGA+TGG 0.565289 8.3:+29797027 None:intergenic
ACTCATGATATGCAGAGCAA+TGG 0.567782 8.3:-29794202 MS.gene99585:CDS
GATTTCCATCTGAAGTATGA+TGG 0.568462 8.3:-29797113 MS.gene99585:CDS
GGATGATTGGTCATTGTACA+AGG 0.571953 8.3:-29797600 MS.gene99585:CDS
AAGTACAACGCTCCATTGCA+AGG 0.577100 8.3:+29796312 None:intergenic
GATGATCATCTCCAAATGGA+TGG 0.581367 8.3:+29795464 None:intergenic
ATATTATGGTCATTCTCCAC+AGG 0.591103 8.3:-29797680 MS.gene99585:CDS
CGAAATCAGAACACTTATGT+CGG 0.592650 8.3:-29796393 MS.gene99585:CDS
GTCAACCAGTTGCAGTCAAG+CGG 0.593335 8.3:-29796448 MS.gene99585:CDS
GCCAGGGAAGGAAGAATGGA+AGG 0.594266 8.3:-29796478 MS.gene99585:CDS
GTTTATTTGAACAAGAAGAG+TGG 0.596440 8.3:-29796692 MS.gene99585:CDS
GTATCGATGAACATTAATGA+AGG 0.600915 8.3:-29796551 MS.gene99585:CDS
TGAGGAATCCAATGATGTTG+TGG 0.601179 8.3:-29794287 MS.gene99585:CDS
TACAAGGAGGATAAGTACTA+CGG 0.605030 8.3:-29797584 MS.gene99585:CDS
ATAACGAACATACTAGGTGA+TGG 0.607629 8.3:+29796659 None:intergenic
GGCTTCCCAACCTGTACAGT+AGG 0.608756 8.3:+29795573 None:intergenic
ACTAATTGATGACCCAATTG+TGG 0.609257 8.3:-29794350 MS.gene99585:CDS
GCTGTAGATTGGCGATCCTG+TGG 0.610761 8.3:+29797664 None:intergenic
TCATACTACATTTCATGTAG+AGG 0.611755 8.3:-29797021 MS.gene99585:CDS
TGTGGACACGCAACACGTTG+AGG 0.612887 8.3:-29794269 MS.gene99585:CDS
AATGACCATAATATCCTCAT+GGG 0.614134 8.3:+29797689 None:intergenic
ACTTGACACCAGTGACAAGA+CGG 0.614987 8.3:-29794374 MS.gene99585:CDS
TAATAATACAAAATCAAACT+CGG 0.615612 8.3:+29797052 None:intergenic
CATTGTCAAATGGCATGGAG+TGG 0.616032 8.3:-29796357 MS.gene99585:CDS
AACATACTAGGTGATGGAGT+TGG 0.616054 8.3:+29796665 None:intergenic
CCAAAATCCTGATCCCGACG+TGG 0.618016 8.3:+29797088 None:intergenic
TAAGTATAAGTATATACCAA+AGG 0.620241 8.3:-29796588 MS.gene99585:CDS
GAAGCCCCAGAGCAGCATCA+AGG 0.621107 8.3:-29795556 MS.gene99585:CDS
GGAGTTCATTCCGGAAGCTG+AGG 0.621667 8.3:-29795392 MS.gene99585:CDS
CAACTGAATCATCATATCTG+AGG 0.628170 8.3:+29796853 None:intergenic
CTATAAATATTGATCAGCAG+CGG 0.630252 8.3:+29797140 None:intergenic
ACTGCTATCTCGTGGTAAAG+GGG 0.632547 8.3:-29796613 MS.gene99585:CDS
AGTGCTTACCAGGAACATGT+TGG 0.639292 8.3:+29795956 None:intergenic
AATTCCTTGATGCTGCTCTG+GGG 0.641432 8.3:+29795552 None:intergenic
AGTAAATCACTCTCAGTGAG+AGG 0.642560 8.3:+29796722 None:intergenic
GAAGTATGATGGTCCCACGT+CGG 0.643813 8.3:-29797102 MS.gene99585:CDS
ATAAATGGTCCCATCAGGTG+CGG 0.657978 8.3:+29797736 None:intergenic
TTAATCAGTGAAAGTTCACA+TGG 0.660628 8.3:-29796917 MS.gene99585:intron
TTCACTCTACTGCTATCTCG+TGG 0.664124 8.3:-29796621 MS.gene99585:CDS
CAAAATCCTGATCCCGACGT+GGG 0.684562 8.3:+29797089 None:intergenic
CATAAACTAGGAATAATACA+CGG 0.689054 8.3:-29796054 MS.gene99585:CDS
TGATTGGTCATTGTACAAGG+AGG 0.729224 8.3:-29797597 MS.gene99585:CDS
TCTACGAGAATGGTCCCATG+AGG 0.737091 8.3:-29797703 MS.gene99585:CDS
TCATGATATGCAGAGCAATG+GGG 0.751619 8.3:-29794200 MS.gene99585:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATTATTCATAACATTAAT+AGG + chr8.3:29796941-29796960 None:intergenic 10.0%
!! TTGAAAATAAAACAAAAATT+AGG + chr8.3:29796362-29796381 None:intergenic 10.0%
!!! AAATTAGTTTTTATATTACA+TGG - chr8.3:29797392-29797411 MS.gene99585:intron 10.0%
!!! GTAATATAAAAACTAATTTT+TGG + chr8.3:29797391-29797410 None:intergenic 10.0%
!!! TTTATAAAAAGTTTAAAGTT+CGG - chr8.3:29797228-29797247 MS.gene99585:intron 10.0%
!! AGATTAATGTTATCAAAATA+GGG + chr8.3:29796984-29797003 None:intergenic 15.0%
!! TAAACAATATAAAGAGAAAA+AGG + chr8.3:29796517-29796536 None:intergenic 15.0%
!! TAATAATACAAAATCAAACT+CGG + chr8.3:29795087-29795106 None:intergenic 15.0%
!! TAGATTAATGTTATCAAAAT+AGG + chr8.3:29796985-29797004 None:intergenic 15.0%
!! TTGAAAAGAAAACAAAAATT+AGG + chr8.3:29796421-29796440 None:intergenic 15.0%
!!! ATTTTTTTTTTGAGGAAATT+TGG - chr8.3:29794761-29794780 MS.gene99585:intron 15.0%
!!! GTAGTTTTATTTGTTTTTAT+TGG - chr8.3:29797007-29797026 MS.gene99585:CDS 15.0%
!!! TAATTATTTTGTTGTAGAAA+AGG + chr8.3:29795369-29795388 None:intergenic 15.0%
!!! TATAATAATAAGAATTTTGC+TGG - chr8.3:29794948-29794967 MS.gene99585:intron 15.0%
!!! TTTTTTTTTTGAGGAAATTT+GGG - chr8.3:29794762-29794781 MS.gene99585:intron 15.0%
!! AGACTAAGATTTATTAGAAT+AGG - chr8.3:29797063-29797082 MS.gene99585:CDS 20.0%
!! ATTCTAATAAATCTTAGTCT+TGG + chr8.3:29797063-29797082 None:intergenic 20.0%
!! CCATTATATGAAATTATGAT+AGG + chr8.3:29794862-29794881 None:intergenic 20.0%
!! CCTATCATAATTTCATATAA+TGG - chr8.3:29794859-29794878 MS.gene99585:intron 20.0%
!! CTCTTTATATTGTTTAAACA+GGG - chr8.3:29796521-29796540 MS.gene99585:CDS 20.0%
!! CTTTATCAATAATATATGCT+AGG - chr8.3:29794240-29794259 MS.gene99585:CDS 20.0%
!! TAAGTATAAGTATATACCAA+AGG - chr8.3:29795548-29795567 MS.gene99585:CDS 20.0%
!! TACAACAAAATAATTACAAG+AGG - chr8.3:29795373-29795392 MS.gene99585:CDS 20.0%
!! TACTTGTAACTTAAATGATT+TGG - chr8.3:29795842-29795861 MS.gene99585:intron 20.0%
!! TAGAACATTGTAATTGTAAT+TGG - chr8.3:29794590-29794609 MS.gene99585:intron 20.0%
!! TATGTTGTATCAAATGATTA+AGG - chr8.3:29796894-29796913 MS.gene99585:CDS 20.0%
!! TCAAAATGTTCTAATAATCA+AGG - chr8.3:29796117-29796136 MS.gene99585:intron 20.0%
!! TCTCTTTATATTGTTTAAAC+AGG - chr8.3:29796520-29796539 MS.gene99585:CDS 20.0%
!! TGTTGAAAAGGAAAATATTA+GGG - chr8.3:29797825-29797844 MS.gene99585:intron 20.0%
!! TTGTTGAAAAGGAAAATATT+AGG - chr8.3:29797824-29797843 MS.gene99585:intron 20.0%
!!! AAATTATTTCCTTTATTTCC+AGG - chr8.3:29797674-29797693 MS.gene99585:CDS 20.0%
!!! CTTGATTTTCCTATTTATTT+AGG - chr8.3:29797486-29797505 MS.gene99585:intron 20.0%
!!! GTTATATCTTATCTTTTGAA+GGG - chr8.3:29797201-29797220 MS.gene99585:intron 20.0%
!!! TGTTATATCTTATCTTTTGA+AGG - chr8.3:29797200-29797219 MS.gene99585:intron 20.0%
!!! TTGATTATTAGAACATTTTG+AGG + chr8.3:29796118-29796137 None:intergenic 20.0%
!!! TTTAGTCTTTTGTATCAAAT+TGG - chr8.3:29797552-29797571 MS.gene99585:intron 20.0%
! AAGAAAAAGAGTTGTTAGAA+CGG + chr8.3:29794214-29794233 None:intergenic 25.0%
! ACTTCTCTATTATTATCCTT+TGG + chr8.3:29795567-29795586 None:intergenic 25.0%
! CATAAACTAGGAATAATACA+CGG - chr8.3:29796082-29796101 MS.gene99585:CDS 25.0%
! CATAACATTAATAGGTATGT+TGG + chr8.3:29796933-29796952 None:intergenic 25.0%
! TAAAAATGAAACTCTCTTCT+GGG + chr8.3:29797743-29797762 None:intergenic 25.0%
! TAAATCATTGTCATTCATTG+TGG - chr8.3:29796808-29796827 MS.gene99585:CDS 25.0%
! TGCTTCTGTAAATTACAATA+TGG - chr8.3:29796275-29796294 MS.gene99585:intron 25.0%
! TGGATACTACAAAATATCTT+TGG - chr8.3:29795883-29795902 MS.gene99585:intron 25.0%
! TTCGTTTCAAAAAAAGAGAT+TGG - chr8.3:29795636-29795655 MS.gene99585:intron 25.0%
! TTGAAAAATCCATCAAAATC+AGG + chr8.3:29795312-29795331 None:intergenic 25.0%
! TTTCCTATTTATTTAGGAGT+AGG - chr8.3:29797492-29797511 MS.gene99585:intron 25.0%
!!! AGATTTTATTTACCTTGCAA+TGG - chr8.3:29795812-29795831 MS.gene99585:intron 25.0%
!!! ATTTTTGTTTTGCAACTTAG+GGG - chr8.3:29796034-29796053 MS.gene99585:CDS 25.0%
!!! CATTTTTGTTTTGCAACTTA+GGG - chr8.3:29796033-29796052 MS.gene99585:CDS 25.0%
!!! CTTTTTTTCTGTTTGACTAA+GGG - chr8.3:29796646-29796665 MS.gene99585:CDS 25.0%
!!! TCTTTTTTTCTGTTTGACTA+AGG - chr8.3:29796645-29796664 MS.gene99585:CDS 25.0%
AAAGGAAAATATTAGGGTTG+AGG - chr8.3:29797831-29797850 MS.gene99585:intron 30.0%
AACAGAATAACGAACATACT+AGG + chr8.3:29795486-29795505 None:intergenic 30.0%
AACATTAGATTCGATCGATA+TGG + chr8.3:29796452-29796471 None:intergenic 30.0%
AATGACCATAATATCCTCAT+GGG + chr8.3:29794450-29794469 None:intergenic 30.0%
ACACGATTATAAACTAATCC+TGG + chr8.3:29797354-29797373 None:intergenic 30.0%
ACATTCACAAGGTAAATGTT+AGG + chr8.3:29796232-29796251 None:intergenic 30.0%
CAATGATCATGATGGTAATA+TGG - chr8.3:29795329-29795348 MS.gene99585:intron 30.0%
CAATGATTTACCTTAGATTC+TGG + chr8.3:29796798-29796817 None:intergenic 30.0%
CACGATTATAAACTAATCCT+GGG + chr8.3:29797353-29797372 None:intergenic 30.0%
CATGAAATGTAGTATGATGA+TGG + chr8.3:29795112-29795131 None:intergenic 30.0%
CCTAAGTTGCAAAACAAAAA+TGG + chr8.3:29796035-29796054 None:intergenic 30.0%
CGATCGAATCTAATGTTATT+CGG - chr8.3:29796455-29796474 MS.gene99585:CDS 30.0%
CTATAAATATTGATCAGCAG+CGG + chr8.3:29794999-29795018 None:intergenic 30.0%
GAGAAAAAGGTCAATGAATT+TGG + chr8.3:29796504-29796523 None:intergenic 30.0%
GCTGATCAATATTTATAGGA+AGG - chr8.3:29795001-29795020 MS.gene99585:intron 30.0%
GTAAAAATGAAACTCTCTTC+TGG + chr8.3:29797744-29797763 None:intergenic 30.0%
GTTGTTCATTTGCATAAACT+AGG - chr8.3:29796070-29796089 MS.gene99585:CDS 30.0%
GTTTATTTGAACAAGAAGAG+TGG - chr8.3:29795444-29795463 MS.gene99585:CDS 30.0%
TAAAAATCTTAGTGCTTACC+AGG + chr8.3:29796193-29796212 None:intergenic 30.0%
TAGTATGTTCGTTATTCTGT+TGG - chr8.3:29795485-29795504 MS.gene99585:CDS 30.0%
TCATACTACATTTCATGTAG+AGG - chr8.3:29795115-29795134 MS.gene99585:intron 30.0%
TTAATCAGTGAAAGTTCACA+TGG - chr8.3:29795219-29795238 MS.gene99585:intron 30.0%
TTTAACTCTCTAGCTAATGT+AGG + chr8.3:29794650-29794669 None:intergenic 30.0%
! ACTTTTTGCTTCTTTAGACA+TGG + chr8.3:29795164-29795183 None:intergenic 30.0%
! CTTTTTGCTTCTTTAGACAT+GGG + chr8.3:29795163-29795182 None:intergenic 30.0%
! GTATCGATGAACATTAATGA+AGG - chr8.3:29795585-29795604 MS.gene99585:CDS 30.0%
! TATAATGGAGAAGCTGTAAT+TGG - chr8.3:29794874-29794893 MS.gene99585:intron 30.0%
! TTCTATATCGATTAGCTCTT+AGG - chr8.3:29796387-29796406 MS.gene99585:CDS 30.0%
!! AACATTAATGAAGGTGTTGA+TGG - chr8.3:29795594-29795613 MS.gene99585:CDS 30.0%
!! AGCTTTAGAGTTTAGATTGA+AGG - chr8.3:29797253-29797272 MS.gene99585:CDS 30.0%
!! CTTAAATGATTTGGTTCAAG+TGG - chr8.3:29795851-29795870 MS.gene99585:intron 30.0%
!! GGATTTTTCAATGATCATGA+TGG - chr8.3:29795321-29795340 MS.gene99585:intron 30.0%
!! TTTTGTTTCTTCGTCAAGTA+TGG + chr8.3:29797979-29797998 None:intergenic 30.0%
!!! CCATTTTTGTTTTGCAACTT+AGG - chr8.3:29796032-29796051 MS.gene99585:CDS 30.0%
!!! GTAGTGTTATTTTGCTTCTT+TGG - chr8.3:29797176-29797195 MS.gene99585:CDS 30.0%
AAAAGAGTTGTTAGAACGGA+CGG + chr8.3:29794210-29794229 None:intergenic 35.0%
ACAAAATAAATGGTCCCATC+AGG + chr8.3:29794408-29794427 None:intergenic 35.0%
ACACTTACAATGCACAAGTA+AGG + chr8.3:29795977-29795996 None:intergenic 35.0%
ATAACGAACATACTAGGTGA+TGG + chr8.3:29795480-29795499 None:intergenic 35.0%
ATATTATGGTCATTCTCCAC+AGG - chr8.3:29794456-29794475 MS.gene99585:intron 35.0%
ATCATCCGAACATTGTCAAA+TGG - chr8.3:29795769-29795788 MS.gene99585:intron 35.0%
ATGTGTAAAGCTTTCTGACA+TGG - chr8.3:29796150-29796169 MS.gene99585:intron 35.0%
ATTTATTTAGGAGTAGGAGC+AGG - chr8.3:29797498-29797517 MS.gene99585:intron 35.0%
ATTTGAACAAGAAGAGTGGA+TGG - chr8.3:29795448-29795467 MS.gene99585:CDS 35.0%
ATTTGACAATGTTCGGATGA+TGG + chr8.3:29795770-29795789 None:intergenic 35.0%
CAAAGATGATCATCTCCAAA+TGG + chr8.3:29796679-29796698 None:intergenic 35.0%
CAACTGAATCATCATATCTG+AGG + chr8.3:29795286-29795305 None:intergenic 35.0%
CACTTACAATGCACAAGTAA+GGG + chr8.3:29795976-29795995 None:intergenic 35.0%
CATTTGGAGATGATCATCTT+TGG - chr8.3:29796677-29796696 MS.gene99585:CDS 35.0%
CGAAATCAGAACACTTATGT+CGG - chr8.3:29795743-29795762 MS.gene99585:intron 35.0%
CGCTGCTGATCAATATTTAT+AGG - chr8.3:29794997-29795016 MS.gene99585:intron 35.0%
CTCTACTACTAAAACTGATG+AGG - chr8.3:29795929-29795948 MS.gene99585:intron 35.0%
CTCTGCATATCATGAGTTAA+AGG + chr8.3:29797931-29797950 None:intergenic 35.0%
GAATGACCATAATATCCTCA+TGG + chr8.3:29794451-29794470 None:intergenic 35.0%
GATAAACTAGCTAGGATACT+AGG + chr8.3:29797329-29797348 None:intergenic 35.0%
GCTCCTACTCCTAAATAAAT+AGG + chr8.3:29797498-29797517 None:intergenic 35.0%
GGAACATCAGATTGTTGAAA+AGG - chr8.3:29797813-29797832 MS.gene99585:intron 35.0%
GTAGATGGCCACAAAATAAA+TGG + chr8.3:29794418-29794437 None:intergenic 35.0%
GTCAGAAAGCTTTACACATA+AGG + chr8.3:29796149-29796168 None:intergenic 35.0%
TAAACTCTGACCAGAATCTA+AGG - chr8.3:29796785-29796804 MS.gene99585:CDS 35.0%
TACAAGGAGGATAAGTACTA+CGG - chr8.3:29794552-29794571 MS.gene99585:intron 35.0%
TCGAAAAGATCTTTCCTTAG+TGG - chr8.3:29796723-29796742 MS.gene99585:CDS 35.0%
TGCTATTGCGTATTTCTTTC+TGG - chr8.3:29797132-29797151 MS.gene99585:CDS 35.0%
TGTGTAAAGCTTTCTGACAT+GGG - chr8.3:29796151-29796170 MS.gene99585:intron 35.0%
TTAGTAGTAGAGGTGATGAA+TGG + chr8.3:29795921-29795940 None:intergenic 35.0%
TTCGTCAAGTATGGTGTAAA+AGG + chr8.3:29797970-29797989 None:intergenic 35.0%
TTTGATGCATTTAGATTCCC+AGG + chr8.3:29794975-29794994 None:intergenic 35.0%
! AAAGATCTTTTCGATCGTTC+AGG + chr8.3:29796717-29796736 None:intergenic 35.0%
! AATAAGAATTTTGCTGGTCC+TGG - chr8.3:29794954-29794973 MS.gene99585:intron 35.0%
! ACTAATTGATGACCCAATTG+TGG - chr8.3:29797786-29797805 MS.gene99585:intron 35.0%
! ATAAGAATTTTGCTGGTCCT+GGG - chr8.3:29794955-29794974 MS.gene99585:intron 35.0%
! ATTTGGGATTGATGTTCTGT+TGG - chr8.3:29794778-29794797 MS.gene99585:intron 35.0%
! GATTTCCATCTGAAGTATGA+TGG - chr8.3:29795023-29795042 MS.gene99585:intron 35.0%
! TCAAAAAAAGAGATTGGCCA+GGG - chr8.3:29795642-29795661 MS.gene99585:intron 35.0%
! TTCAAAAAAAGAGATTGGCC+AGG - chr8.3:29795641-29795660 MS.gene99585:intron 35.0%
! TTTTCGATCGTTCAGGATAT+TGG + chr8.3:29796710-29796729 None:intergenic 35.0%
!! CTCATCAGTTTTAGTAGTAG+AGG + chr8.3:29795931-29795950 None:intergenic 35.0%
!! GGTGTTGATGGAGAAATAAT+TGG - chr8.3:29795606-29795625 MS.gene99585:intron 35.0%
!! TTCAAGTTGAAGCCAAAACT+GGG - chr8.3:29794917-29794936 MS.gene99585:intron 35.0%
!! TTTATTGGTCTGCTGATTTC+AGG - chr8.3:29797022-29797041 MS.gene99585:CDS 35.0%
!! TTTCAAGTTGAAGCCAAAAC+TGG - chr8.3:29794916-29794935 MS.gene99585:intron 35.0%
!! TTTTGTTGTAGAAAAGGCGT+AGG + chr8.3:29795363-29795382 None:intergenic 35.0%
!!! GTCATTCATTGTGGTTTTAG+AGG - chr8.3:29796817-29796836 MS.gene99585:CDS 35.0%
!!! TGATGGGACCATTTATTTTG+TGG - chr8.3:29794407-29794426 MS.gene99585:CDS 35.0%
AATCCCATGCTAGGCATAAT+TGG + chr8.3:29796255-29796274 None:intergenic 40.0%
AATTAGTTCCGTCTTGTCAC+TGG + chr8.3:29797773-29797792 None:intergenic 40.0%
ACTCATGATATGCAGAGCAA+TGG - chr8.3:29797934-29797953 MS.gene99585:intron 40.0%
AGTAAATCACTCTCAGTGAG+AGG + chr8.3:29795417-29795436 None:intergenic 40.0%
AGTCTTGAAAAAGCGAGAAG+AGG + chr8.3:29794158-29794177 None:intergenic 40.0%
AGTTCACATGGTGCTTATCA+TGG - chr8.3:29795231-29795250 MS.gene99585:intron 40.0%
ATGGTCCCATGAGGATATTA+TGG - chr8.3:29794442-29794461 MS.gene99585:CDS 40.0%
ATGTCCAATTATGCCTAGCA+TGG - chr8.3:29796248-29796267 MS.gene99585:intron 40.0%
CAAATCCTACTGTACAGGTT+GGG - chr8.3:29796558-29796577 MS.gene99585:CDS 40.0%
CTAATAAGATCCTCAGCTTC+CGG + chr8.3:29796757-29796776 None:intergenic 40.0%
CTCACTGAGAGTGATTTACT+AGG - chr8.3:29795417-29795436 MS.gene99585:CDS 40.0%
CTCATGATATGCAGAGCAAT+GGG - chr8.3:29797935-29797954 MS.gene99585:intron 40.0%
GATGATCATCTCCAAATGGA+TGG + chr8.3:29796675-29796694 None:intergenic 40.0%
GATGTTCCTTGACCACAATT+GGG + chr8.3:29797801-29797820 None:intergenic 40.0%
GCTGCAGACATATTCAGTTT+AGG - chr8.3:29796616-29796635 MS.gene99585:CDS 40.0%
GGCATAATTGGACATTCACA+AGG + chr8.3:29796243-29796262 None:intergenic 40.0%
TACTGCTATCTCGTGGTAAA+GGG - chr8.3:29795522-29795541 MS.gene99585:CDS 40.0%
TCATGATATGCAGAGCAATG+GGG - chr8.3:29797936-29797955 MS.gene99585:intron 40.0%
TGAGGAATCCAATGATGTTG+TGG - chr8.3:29797849-29797868 MS.gene99585:intron 40.0%
TGATGTTCCTTGACCACAAT+TGG + chr8.3:29797802-29797821 None:intergenic 40.0%
TGCAACACATCAATTGCGTT+CGG + chr8.3:29797700-29797719 None:intergenic 40.0%
TGCTTCTTTAGACATGGGTA+AGG + chr8.3:29795158-29795177 None:intergenic 40.0%
TGTCCAATTATGCCTAGCAT+GGG - chr8.3:29796249-29796268 MS.gene99585:intron 40.0%
TTCCTTAGTGGAGTTCATTC+CGG - chr8.3:29796735-29796754 MS.gene99585:CDS 40.0%
! AAACTCTCTTCTGGGAATTC+CGG + chr8.3:29797735-29797754 None:intergenic 40.0%
! AGGAAGAATGGAAGGTATGA+TGG - chr8.3:29795666-29795685 MS.gene99585:intron 40.0%
! ATTTGGTTCAAGTGGAGTCA+GGG - chr8.3:29795859-29795878 MS.gene99585:intron 40.0%
! CCATGCCATTTGACAATGTT+CGG + chr8.3:29795777-29795796 None:intergenic 40.0%
! GGATGATTGGTCATTGTACA+AGG - chr8.3:29794536-29794555 MS.gene99585:intron 40.0%
! TGAATCATCATATCTGAGGC+AGG + chr8.3:29795282-29795301 None:intergenic 40.0%
! TGATTGGTCATTGTACAAGG+AGG - chr8.3:29794539-29794558 MS.gene99585:intron 40.0%
! TTCTGGGAATTCCGGAAAAA+AGG + chr8.3:29797727-29797746 None:intergenic 40.0%
! TTTTGGACTGTGAGGATGAT+TGG - chr8.3:29794523-29794542 MS.gene99585:intron 40.0%
!! AACATACTAGGTGATGGAGT+TGG + chr8.3:29795474-29795493 None:intergenic 40.0%
!! AACTTAGGGGTATTGTTGCA+GGG - chr8.3:29796047-29796066 MS.gene99585:CDS 40.0%
!! TAGCGAATTTTTGGACTGTG+AGG - chr8.3:29794515-29794534 MS.gene99585:intron 40.0%
!!! TGTTGCAGCATCCTTTTTTC+CGG - chr8.3:29797713-29797732 MS.gene99585:CDS 40.0%
AAGTACAACGCTCCATTGCA+AGG + chr8.3:29795827-29795846 None:intergenic 45.0%
AATTCCTTGATGCTGCTCTG+GGG + chr8.3:29796587-29796606 None:intergenic 45.0%
ACAGAAGCAAATCCCATGCT+AGG + chr8.3:29796264-29796283 None:intergenic 45.0%
ACTAAGGGAAGCCATCCATT+TGG - chr8.3:29796661-29796680 MS.gene99585:CDS 45.0%
ACTGCTATCTCGTGGTAAAG+GGG - chr8.3:29795523-29795542 MS.gene99585:CDS 45.0%
ACTTGACACCAGTGACAAGA+CGG - chr8.3:29797762-29797781 MS.gene99585:CDS 45.0%
AGGAAGAGGAACAGAAACCA+GGG + chr8.3:29794138-29794157 None:intergenic 45.0%
AGTGCTTACCAGGAACATGT+TGG + chr8.3:29796183-29796202 None:intergenic 45.0%
AGTTGAAGCCAAAACTGGGT+CGG - chr8.3:29794921-29794940 MS.gene99585:intron 45.0%
ATAAATGGTCCCATCAGGTG+CGG + chr8.3:29794403-29794422 None:intergenic 45.0%
ATTTGCCGATCTCACGTAGT+AGG - chr8.3:29797417-29797436 MS.gene99585:intron 45.0%
CAATTCCTTGATGCTGCTCT+GGG + chr8.3:29796588-29796607 None:intergenic 45.0%
CATTGTCAAATGGCATGGAG+TGG - chr8.3:29795779-29795798 MS.gene99585:intron 45.0%
CCAAATCCTACTGTACAGGT+TGG - chr8.3:29796557-29796576 MS.gene99585:CDS 45.0%
CCAACCTGTACAGTAGGATT+TGG + chr8.3:29796560-29796579 None:intergenic 45.0%
CCGAACATTGTCAAATGGCA+TGG - chr8.3:29795774-29795793 MS.gene99585:intron 45.0%
CTACTGCTATCTCGTGGTAA+AGG - chr8.3:29795521-29795540 MS.gene99585:CDS 45.0%
GAAAAAGCGAGAAGAGGAAG+AGG + chr8.3:29794152-29794171 None:intergenic 45.0%
GCTTGATACGAGCTGTAGAT+TGG + chr8.3:29794486-29794505 None:intergenic 45.0%
GGATAAGTACTACGGCAAAC+TGG - chr8.3:29794560-29794579 MS.gene99585:intron 45.0%
GGCATTAACCAACATGTTCC+TGG - chr8.3:29796172-29796191 MS.gene99585:intron 45.0%
GTCCAAAAATTCGCTAGCTC+CGG + chr8.3:29794511-29794530 None:intergenic 45.0%
TCAATTCCTTGATGCTGCTC+TGG + chr8.3:29796589-29796608 None:intergenic 45.0%
TCCAAAAATTCGCTAGCTCC+GGG + chr8.3:29794510-29794529 None:intergenic 45.0%
TCCTGGGAGATAAACTAGCT+AGG + chr8.3:29797337-29797356 None:intergenic 45.0%
TGATGACCCAATTGTGGTCA+AGG - chr8.3:29797792-29797811 MS.gene99585:intron 45.0%
TGGGACCATCATACTTCAGA+TGG + chr8.3:29795031-29795050 None:intergenic 45.0%
TTCACTCTACTGCTATCTCG+TGG - chr8.3:29795515-29795534 MS.gene99585:CDS 45.0%
TTCCGGAATGAACTCCACTA+AGG + chr8.3:29796740-29796759 None:intergenic 45.0%
TTCGCCAAATCCTACTGTAC+AGG - chr8.3:29796553-29796572 MS.gene99585:CDS 45.0%
TTGCGTGTCCACAACATCAT+TGG + chr8.3:29797860-29797879 None:intergenic 45.0%
TTTGCCGATCTCACGTAGTA+GGG - chr8.3:29797418-29797437 MS.gene99585:intron 45.0%
TTTGTGGCCATCTACGAGAA+TGG - chr8.3:29794423-29794442 MS.gene99585:CDS 45.0%
! AAAAAGAGATTGGCCAGGGA+AGG - chr8.3:29795646-29795665 MS.gene99585:intron 45.0%
! CAGTTGCAGCCTGATTTTGA+TGG - chr8.3:29795300-29795319 MS.gene99585:intron 45.0%
! GATTTGGTTCAAGTGGAGTC+AGG - chr8.3:29795858-29795877 MS.gene99585:intron 45.0%
! TATACTGACCGACCCAGTTT+TGG + chr8.3:29794932-29794951 None:intergenic 45.0%
! TCCTAGCTAGTTTATCTCCC+AGG - chr8.3:29797333-29797352 MS.gene99585:intron 45.0%
! TGTTGTAGAAAAGGCGTAGG+AGG + chr8.3:29795360-29795379 None:intergenic 45.0%
!! ACTTAGGGGTATTGTTGCAG+GGG - chr8.3:29796048-29796067 MS.gene99585:CDS 45.0%
!! ATTGGTCTGCTGATTTCAGG+AGG - chr8.3:29797025-29797044 MS.gene99585:CDS 45.0%
!! CAACTTAGGGGTATTGTTGC+AGG - chr8.3:29796046-29796065 MS.gene99585:CDS 45.0%
!!! TAATATAAAAACTAATTTTT+GGG + chr8.3:29797390-29797409 None:intergenic 5.0%
!!! TTTATTTTATTTTTTTTTTG+AGG - chr8.3:29794753-29794772 MS.gene99585:intron 5.0%
AAACTGTTTGCCGCACCTGA+TGG - chr8.3:29794390-29794409 MS.gene99585:CDS 50.0%
AACTGTTTGCCGCACCTGAT+GGG - chr8.3:29794391-29794410 MS.gene99585:CDS 50.0%
AAGTATGATGGTCCCACGTC+GGG - chr8.3:29795035-29795054 MS.gene99585:intron 50.0%
ACCTGATCAGGTACAAGAGC+AGG - chr8.3:29797894-29797913 MS.gene99585:intron 50.0%
ACCTGCTCTTGTACCTGATC+AGG + chr8.3:29797898-29797917 None:intergenic 50.0%
ACCTTCCATTCTTCCTTCCC+TGG + chr8.3:29795662-29795681 None:intergenic 50.0%
AGAGTTGTTAGAACGGACGG+AGG + chr8.3:29794207-29794226 None:intergenic 50.0%
ATTGGCCAGGGAAGGAAGAA+TGG - chr8.3:29795654-29795673 MS.gene99585:intron 50.0%
CAAAATCCTGATCCCGACGT+GGG + chr8.3:29795050-29795069 None:intergenic 50.0%
CATGGGACCATTCTCGTAGA+TGG + chr8.3:29794433-29794452 None:intergenic 50.0%
CGTTGAGGTTTCACCTGATC+AGG - chr8.3:29797882-29797901 MS.gene99585:intron 50.0%
CTGACCCTACTACGTGAGAT+CGG + chr8.3:29797425-29797444 None:intergenic 50.0%
GAAGTATGATGGTCCCACGT+CGG - chr8.3:29795034-29795053 MS.gene99585:intron 50.0%
GAGGAAGAGGAACAGAAACC+AGG + chr8.3:29794139-29794158 None:intergenic 50.0%
GGAAGAGGAACAGAAACCAG+GGG + chr8.3:29794137-29794156 None:intergenic 50.0%
GTCAACCAGTTGCAGTCAAG+CGG - chr8.3:29795688-29795707 MS.gene99585:intron 50.0%
TACAGCTCGTATCAAGCTCC+CGG - chr8.3:29794489-29794508 MS.gene99585:intron 50.0%
TCTACGAGAATGGTCCCATG+AGG - chr8.3:29794433-29794452 MS.gene99585:CDS 50.0%
TGCGTTCGGCCTGGAAATAA+AGG + chr8.3:29797686-29797705 None:intergenic 50.0%
! TCCCGGAGCTAGCGAATTTT+TGG - chr8.3:29794506-29794525 MS.gene99585:intron 50.0%
!! GGTGCGGCAAACAGTTTTGT+TGG + chr8.3:29794387-29794406 None:intergenic 50.0%
AGAAGCCGCTTGACTGCAAC+TGG + chr8.3:29795696-29795715 None:intergenic 55.0%
CACATCAATTGCGTTCGGCC+TGG + chr8.3:29797695-29797714 None:intergenic 55.0%
CCAAAATCCTGATCCCGACG+TGG + chr8.3:29795051-29795070 None:intergenic 55.0%
CGATCTCACGTAGTAGGGTC+AGG - chr8.3:29797423-29797442 MS.gene99585:intron 55.0%
GCCAGGGAAGGAAGAATGGA+AGG - chr8.3:29795658-29795677 MS.gene99585:intron 55.0%
GCTGTAGATTGGCGATCCTG+TGG + chr8.3:29794475-29794494 None:intergenic 55.0%
GGAGTTCATTCCGGAAGCTG+AGG - chr8.3:29796744-29796763 MS.gene99585:CDS 55.0%
GGCTTCCCAACCTGTACAGT+AGG + chr8.3:29796566-29796585 None:intergenic 55.0%
GGTTCAAGTGGAGTCAGGGA+TGG - chr8.3:29795863-29795882 MS.gene99585:intron 55.0%
GTTGTTAGAACGGACGGAGG+AGG + chr8.3:29794204-29794223 None:intergenic 55.0%
TGTGGACACGCAACACGTTG+AGG - chr8.3:29797867-29797886 MS.gene99585:intron 55.0%
! CCACGTCGGGATCAGGATTT+TGG - chr8.3:29795048-29795067 MS.gene99585:intron 55.0%
GAAGCCCCAGAGCAGCATCA+AGG - chr8.3:29796580-29796599 MS.gene99585:CDS 60.0%
GTTAGAACGGACGGAGGAGG+AGG + chr8.3:29794201-29794220 None:intergenic 60.0%
ACGGAGGAGGAGGAGGAGCA+TGG + chr8.3:29794191-29794210 None:intergenic 65.0%
AGAACGGACGGAGGAGGAGG+AGG + chr8.3:29794198-29794217 None:intergenic 65.0%
GATGGTCCCACGTCGGGATC+AGG - chr8.3:29795041-29795060 MS.gene99585:intron 65.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 29794123 29798035 29794123 ID=MS.gene99585
chr8.3 mRNA 29794123 29798035 29794123 ID=MS.gene99585.t1;Parent=MS.gene99585
chr8.3 exon 29798019 29798035 29798019 ID=MS.gene99585.t1.exon1;Parent=MS.gene99585.t1
chr8.3 CDS 29798019 29798035 29798019 ID=cds.MS.gene99585.t1;Parent=MS.gene99585.t1
chr8.3 exon 29797577 29797796 29797577 ID=MS.gene99585.t1.exon2;Parent=MS.gene99585.t1
chr8.3 CDS 29797577 29797796 29797577 ID=cds.MS.gene99585.t1;Parent=MS.gene99585.t1
chr8.3 exon 29797237 29797338 29797237 ID=MS.gene99585.t1.exon3;Parent=MS.gene99585.t1
chr8.3 CDS 29797237 29797338 29797237 ID=cds.MS.gene99585.t1;Parent=MS.gene99585.t1
chr8.3 exon 29796975 29797198 29796975 ID=MS.gene99585.t1.exon4;Parent=MS.gene99585.t1
chr8.3 CDS 29796975 29797198 29796975 ID=cds.MS.gene99585.t1;Parent=MS.gene99585.t1
chr8.3 exon 29796284 29796931 29796284 ID=MS.gene99585.t1.exon5;Parent=MS.gene99585.t1
chr8.3 CDS 29796284 29796931 29796284 ID=cds.MS.gene99585.t1;Parent=MS.gene99585.t1
chr8.3 exon 29795965 29796104 29795965 ID=MS.gene99585.t1.exon6;Parent=MS.gene99585.t1
chr8.3 CDS 29795965 29796104 29795965 ID=cds.MS.gene99585.t1;Parent=MS.gene99585.t1
chr8.3 exon 29795352 29795616 29795352 ID=MS.gene99585.t1.exon7;Parent=MS.gene99585.t1
chr8.3 CDS 29795352 29795616 29795352 ID=cds.MS.gene99585.t1;Parent=MS.gene99585.t1
chr8.3 exon 29794123 29794462 29794123 ID=MS.gene99585.t1.exon8;Parent=MS.gene99585.t1
chr8.3 CDS 29794123 29794462 29794123 ID=cds.MS.gene99585.t1;Parent=MS.gene99585.t1
Gene Sequence

>MS.gene99585

ATGAAAGTTCTCCCCTGGTTTCTGTTCCTCTTCCTCTTCTCGCTTTTTCAAGACTGTTGCTGCGACCATGCTCCTCCTCCTCCTCCGTCCGTTCTAACAACTCTTTTTCTTTCTTTTCTTTATCAATAATATATGCTAGGCTTGTTTGATTTGTACATGATATCTTATTATACAATTATTTTTTACTATTTTGATGTTGATATTCTCGTACTTATTTGTGCAAATGTTTTCATTGACAGTAATGAAGCAACAAAACTAATTCCAACAAAACTGTTTGCCGCACCTGATGGGACCATTTATTTTGTGGCCATCTACGAGAATGGTCCCATGAGGATATTATGGTCATTCTCCACAGGATCGCCAATCTACAGCTCGTATCAAGCTCCCGGAGCTAGCGAATTTTTGGACTGTGAGGATGATTGGTCATTGTACAAGGAGGATAAGTACTACGGCAAACTGGTATATGTTAGAACATTGTAATTGTAATTGGTATTTGAAATATGCGTTTTGCAGCATCAGCTTAACCTACATTAGCTAGAGAGTTAAACTTCACTACTGTGTCTGTAACTAACTAAATACAAAGAATATTTGAAGTAGAAAACTAATCTATTCTTACACTGTTTATTCAATTTTATTTTATTTTTTTTTTGAGGAAATTTGGGATTGATGTTCTGTTGGTTGTTTATCATGTCTGCAGATAATAGTAGAATCTATTAGAGAACTTGCACATCGTACGCCTATCATAATTTCATATAATGGAGAAGCTGTAATTGGATCTGAAAAAAGCACTTCGTTTCAAGTTGAAGCCAAAACTGGGTCGGTCAGTATAATAATAAGAATTTTGCTGGTCCTGGGAATCTAAATGCATCAAAGCCGCTGCTGATCAATATTTATAGGAAGGATTTCCATCTGAAGTATGATGGTCCCACGTCGGGATCAGGATTTTGGAATTTGACAGTAGCCGAGTTTGATTTTGTATTATTATGCCATCATCATACTACATTTCATGTAGAGGATCTCAATTTTACAATGCCTTACCCATGTCTAAAGAAGCAAAAAGTGTTCAAACTGAATAAGAACTTTCTGTTTAGAATCTTTAATCAGTGAAAGTTCACATGGTGCTTATCATGGAAATGATACACTCTCAAATTCAATGCCTGCCTCAGATATGATGATTCAGTTGCAGCCTGATTTTGATGGATTTTTCAATGATCATGATGGTAATATGGCAATGCCTCCTACGCCTTTTCTACAACAAAATAATTACAAGAGGAAAGATAAACTTCGTCTGCCTCTCACTGAGAGTGATTTACTAGGACATGTTTATTTGAACAAGAAGAGTGGATGGCCAACTCCATCACCTAGTATGTTCGTTATTCTGTTGGTCGTCGTTTCACTCTACTGCTATCTCGTGGTAAAGGGGATTAAGTATAAGTATATACCAAAGGATAATAATAGAGAAGTATCGATGAACATTAATGAAGGTGTTGATGGAGAAATAATTGGCGAATTATTCGTTTCAAAAAAAGAGATTGGCCAGGGAAGGAAGAATGGAAGGTATGATGGTCAACCAGTTGCAGTCAAGCGGCTTCTCAAATCTCATCATAGTCTAGCTCTAAACGAAATCAGAACACTTATGTCGGACCATCATCCGAACATTGTCAAATGGCATGGAGTGGAGTACGATGAAGATTTTATTTACCTTGCAATGGAGCGTTGTACTTGTAACTTAAATGATTTGGTTCAAGTGGAGTCAGGGATGGATACTACAAAATATCTTTGGAAAAAAAAATGACCATTCATCACCTCTACTACTAAAACTGATGAGGTTTGCATTATTTCTTCTTGAACCCTTACTTGTGCATTGTAAGTGTTGTTTATTTTACACATTTTTAGTATAAACTAACTTCCATTTTTGTTTTGCAACTTAGGGGTATTGTTGCAGGGGTTGTTCATTTGCATAAACTAGGAATAATACACGGAAATTTGAAACCTCAAAATGTTCTAATAATCAAGGATAGATCCTTATGTGTAAAGCTTTCTGACATGGGCATTAACCAACATGTTCCTGGTAAGCACTAAGATTTTTAAAAAAAATTTACATTTCCTAACATTTACCTTGTGAATGTCCAATTATGCCTAGCATGGGATTTGCTTCTGTAAATTACAATATGGTGATTTGTTAGATATCTCTTAATATCTTCAAAGTACTTTAATTGTCTCATAAGAGTTGTTTCCTAATTTTTGTTTTATTTTCAAAAATATTCTATATCGATTAGCTCTTAGGAATTGTTTCCTAATTTTTGTTTTCTTTTCAAATTTGTTTCCATATCGATCGAATCTAATGTTATTCGGATTTGTTTCTCTTCTTTCTCTTTCCAAATTCATTGACCTTTTTCTCTTTATATTGTTTAAACAGGGAAGTCTGTTTTCGCCAAATCCTACTGTACAGGTTGGGAAGCCCCAGAGCAGCATCAAGGAATTGAAACACAAGCTGCAGACATATTCAGTTTAGGTTGTATTCTTTTTTTCTGTTTGACTAAGGGAAGCCATCCATTTGGAGATGATCATCTTTGGCGTGAGTCCAATATCCTGAACGATCGAAAAGATCTTTCCTTAGTGGAGTTCATTCCGGAAGCTGAGGATCTTATTAGCTGTTTATTAAACTCTGACCAGAATCTAAGGTAAATCATTGTCATTCATTGTGGTTTTAGAGGTAATATCTTCTCATACTTTCACAGTGTTGCATCTGCTATGACTTGCTTAAATGTTATGTTGTATCAAATGATTAAGGCTCAAAGTTAATACCAACATACCTATTAATGTTATGAATAATAATTTAAGATGTAAGAGTGTTACCCTATTTTGATAACATTAATCTATTGTAGTTTTATTTGTTTTTATTGGTCTGCTGATTTCAGGAGGTCTTATTTTAAGCCAAGACTAAGATTTATTAGAATAGGTTAAATTTAAATTATGCTATTTCAATATAATTTCAGTCTCTTGCTTTGCTATTGCGTATTTCTTTCTGGTTTGTTATTTCAGTCTCTATTGTAGTGTTATTTTGCTTCTTTGGTTGTTATATCTTATCTTTTGAAGGGAATATTTATAAAAAGTTTAAAGTTCGGTAAGCTTTAGAGTTTAGATTGAAGGATAATGTTAGAATATAAAAAAAAAATGTTATAATATCTTCGATATTATGTCCTAGTATCCTAGCTAGTTTATCTCCCAGGATTAGTTTATAATCGTGTAGCGTCTTTTAGACCCAAAAATTAGTTTTTATATTACATGGTTATTTGCCGATCTCACGTAGTAGGGTCAGGTTTGATTGTTGTATTACACAGTTATTATCATGATTTGTTTCTTGATTTTCCTATTTATTTAGGAGTAGGAGCAGGAGCTTTGTATTTATATAAATAAGAGTTTGTGTTTAGTCTTTTGTATCAAATTGGTAGAAAGTAAATCAATCATATTACATTATTATCATATTCTAAGTCTTTTATTATTTCTCTTCTTTTTTCTAAATCTCAATAACTTCAACAATCATAAAGAAATTATTTCCTTTATTTCCAGGCCGAACGCAATTGATGTGTTGCAGCATCCTTTTTTCCGGAATTCCCAGAAGAGAGTTTCATTTTTACTTGACACCAGTGACAAGACGGAACTAATTGATGACCCAATTGTGGTCAAGGAACATCAGATTGTTGAAAAGGAAAATATTAGGGTTGAGGAATCCAATGATGTTGTGGACACGCAACACGTTGAGGTTTCACCTGATCAGGTACAAGAGCAGGTTCTAAACTCTCCTTTAACTCATGATATGCAGAGCAATGGGGCAGAAGTGCCTTTTACACCATACTTGACGAAGAAACAAAAAAAAACAACTCAAAAGAGCATACAAAACTCGTTTTAA
Protein sequence

>MS.gene99585.t1

MKVLPCNEATKLIPTKLFAAPDGTIYFVAIYENGPMRILWSFSTGSPIYSSYQAPGASEFLDCEDDWSLYKEDKYYGKLIIVESIRELAHRTPIIISYNGEAVIGSEKSTSFQNFAGPGNLNASKPLLINIYRKDFHLKYDGPTSGSGFWNLTVAEFDFVLLCHHHTTFHVEDLNFTMPYPCLKKQKVESSHGAYHGNDTLSNSMPASDMMIQLQPDFDGFFNDHDGNMAMPPTPFLQQNNYKRKDKLRLPLTESDLLGHVYLNKKSGWPTPSPSMFVILLVVVSLYCYLVVKGIKYKYIPKDNNREVSMNINEGVDGEIIGELFVSKKEIGQGRKNGRYDGQPVAVKRLLKSHHSLALNEIRTLMSDHHPNIVKWHGVEYDEDFIYLAMERCTCNLNDLVQVEGIVAGVVHLHKLGIIHGNLKPQNVLIIKDRSLCVKLSDMGINQHVPGKSVFAKSYCTGWEAPEQHQGIETQAADIFSLGCILFFCLTKGSHPFGDDHLWRESNILNDRKDLSLVEFIPEAEDLISCLLNSDQNLRPNAIDVLQHPFFRNSQKRVSFLLDTSDKTELIDDPIVVKEHQIVEKENIRVEESNDVVDTQHVEVSPDQVQEQVLNSPLTHDMQSNGAEVPFTPYLTKKQKKTTQKSIQNSF