AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99749

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99749.t1 MTR_2g061450 88.736 435 46 2 14 448 1 432 0.0 775
MS.gene99749.t1 MTR_2g060620 92.523 107 6 1 318 424 61 165 1.01e-62 201
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99749.t1 AT1G74120 49.548 442 219 2 6 447 7 444 1.43e-149 433
MS.gene99749.t1 AT5G54180 28.829 222 152 5 153 370 253 472 1.52e-15 79.3
MS.gene99749.t1 AT1G62010 27.083 192 121 5 211 399 213 388 7.73e-12 67.4
MS.gene99749.t1 AT5G23930 22.047 381 252 12 45 404 89 445 9.80e-11 63.9

Find 120 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCCCAATTTCTACTTAAT+TGG 0.102106 2.1:+34731278 MS.gene99749:CDS
GAAAGCTTGGATATGAATTT+TGG 0.127070 2.1:-34731313 None:intergenic
ATTCCCAATTAAGTAGAAAT+TGG 0.160887 2.1:-34731282 None:intergenic
TAGATTGTTTCCTAGGGTTT+TGG 0.199228 2.1:+34731576 MS.gene99749:CDS
AATCTTGAATAAGGGAAATT+AGG 0.210110 2.1:-34731240 None:intergenic
TGATGTTGTGATTAGGGTTT+TGG 0.225835 2.1:+34731450 MS.gene99749:CDS
AAATATCAAGTGGGTTTAAA+TGG 0.226943 2.1:-34731387 None:intergenic
TGTTAGGGATCCAAGGGTTT+TGG 0.241406 2.1:+34731684 MS.gene99749:CDS
TTCTGATGATGTTGTGATTA+GGG 0.254350 2.1:+34731444 MS.gene99749:CDS
ATTCATGAGTTGAGGAGTTT+GGG 0.255104 2.1:+34731631 MS.gene99749:CDS
CAGAAGGGCAATCTTGAATA+AGG 0.272691 2.1:-34731249 None:intergenic
ATTTCTACTTAATTGGGAAT+TGG 0.276747 2.1:+34731285 MS.gene99749:CDS
AGGTCAGGGCGGAGGTTGTT+AGG 0.284177 2.1:+34731668 MS.gene99749:CDS
TTTCTGATGATGTTGTGATT+AGG 0.294754 2.1:+34731443 MS.gene99749:CDS
TTCCCAATTAAGTAGAAATT+GGG 0.301013 2.1:-34731281 None:intergenic
CAACACCATATTTCATCCTT+TGG 0.306291 2.1:-34731951 None:intergenic
TTAATCCAAAGGATGAAATA+TGG 0.315058 2.1:+34731946 MS.gene99749:CDS
TTGAAAGGAAAAGGTGCAAT+TGG 0.315697 2.1:+34732054 MS.gene99749:CDS
TGTAGAACACCGCAATAAAT+TGG 0.316256 2.1:-34731359 None:intergenic
GATTCATGAGTTGAGGAGTT+TGG 0.321130 2.1:+34731630 MS.gene99749:CDS
TTTCTACTTAATTGGGAATT+GGG 0.329864 2.1:+34731286 MS.gene99749:CDS
TTTCCCACTGCAGTTGTAAT+GGG 0.336217 2.1:+34731478 MS.gene99749:CDS
GAAGAACATGAAGGCTTTCA+TGG 0.341403 2.1:+34732269 MS.gene99749:CDS
AAGTGGTTCTTACAAGAATA+AGG 0.352405 2.1:-34731000 None:intergenic
GGGTTGTTAGATTGTTTCCT+AGG 0.353615 2.1:+34731569 MS.gene99749:CDS
ACCCCAATTTCTACTTAATT+GGG 0.360043 2.1:+34731279 MS.gene99749:CDS
CATGAAGTTCCTTCATACTT+AGG 0.364333 2.1:+34731982 MS.gene99749:CDS
AAGGTTGATTGCTTACGGTT+TGG 0.397737 2.1:+34731897 MS.gene99749:CDS
GATCCGTAGGGATGCTTTGA+AGG 0.398149 2.1:+34731858 MS.gene99749:CDS
GGTGCAATTGGGTTTGAAGT+TGG 0.399622 2.1:+34732066 MS.gene99749:CDS
TTCCTAAGGATGAAATTGAT+AGG 0.405215 2.1:+34731548 MS.gene99749:CDS
GTAAGCATCCAGCTGGACTT+TGG 0.424175 2.1:+34732202 MS.gene99749:CDS
TGAAAGGAAAAGGTGCAATT+GGG 0.424464 2.1:+34732055 MS.gene99749:CDS
ATTTCCCACTGCAGTTGTAA+TGG 0.426062 2.1:+34731477 MS.gene99749:CDS
GCTAATCTCTTCCAATCCTT+CGG 0.426075 2.1:+34731097 MS.gene99749:CDS
CATGTTCTTCACATCTTGAC+CGG 0.428680 2.1:-34732256 None:intergenic
TGTTGCAGAGTTTGAAATGC+AGG 0.431814 2.1:+34731743 MS.gene99749:CDS
CACAATCTGTCCAATGGGGT+TGG 0.434427 2.1:+34730946 None:intergenic
TGGGTTTGAAGTTGGATTGA+AGG 0.437194 2.1:+34732074 MS.gene99749:CDS
TGTGGAATTTGGGGTTCCTA+AGG 0.437466 2.1:+34731534 MS.gene99749:CDS
TTCACATCTTGACCGGTTTG+AGG 0.446780 2.1:-34732249 None:intergenic
ACAATCTGTCCAATGGGGTT+GGG 0.449876 2.1:+34730947 None:intergenic
CAACCTCCGCCCTGACCTCT+TGG 0.454589 2.1:-34731663 None:intergenic
GAACCAAGGTTGATTGCTTA+CGG 0.455676 2.1:+34731892 MS.gene99749:CDS
TACTCAATCACATTGTATCT+AGG 0.456654 2.1:-34732030 None:intergenic
GGTAAGGGATTGACGAAGGT+GGG 0.465503 2.1:-34731185 None:intergenic
GTTGTTATTAGTTGAAGAAG+TGG 0.465877 2.1:-34731017 None:intergenic
ATCTCCATTCCCAAAACCCT+TGG 0.472530 2.1:-34731694 None:intergenic
GGTCAGGGCGGAGGTTGTTA+GGG 0.473073 2.1:+34731669 MS.gene99749:CDS
TGAAGGAAAAGAGGATGGTA+AGG 0.473436 2.1:-34731201 None:intergenic
GTGGTCACGGTTTGATCCGT+AGG 0.474464 2.1:+34731845 MS.gene99749:CDS
ACCCTATCAATTTCATCCTT+AGG 0.474968 2.1:-34731550 None:intergenic
ATTGAGTATTTGAAAGGAAA+AGG 0.475963 2.1:+34732045 MS.gene99749:CDS
CTCCGCCCTGACCTCTTGGC+TGG 0.476936 2.1:-34731659 None:intergenic
GATCGGTATTGAAGAGGAAG+TGG 0.483347 2.1:-34731159 None:intergenic
GTTGATTGCTTACGGTTTGG+AGG 0.485609 2.1:+34731900 MS.gene99749:CDS
AGGAAGGGAAACCGAAGGAT+TGG 0.491372 2.1:-34731108 None:intergenic
TGGGATTTGGAATTGAGGAT+AGG 0.491709 2.1:+34731596 MS.gene99749:CDS
CAGCCAAGAGGTCAGGGCGG+AGG 0.492996 2.1:+34731660 MS.gene99749:CDS
TGGTAAGGGATTGACGAAGG+TGG 0.495712 2.1:-34731186 None:intergenic
GGTTGTTAGATTGTTTCCTA+GGG 0.496449 2.1:+34731570 MS.gene99749:CDS
TCAAGATTGCCCTTCTGTGT+TGG 0.502454 2.1:+34731255 MS.gene99749:CDS
CGGGGAAGGATTAGAAAGCT+TGG 0.503315 2.1:-34731326 None:intergenic
AATGTGATTGAGTATTTGAA+AGG 0.505696 2.1:+34732039 MS.gene99749:CDS
TTGTAAAATCTAAGCCTAGT+TGG 0.506128 2.1:-34732108 None:intergenic
AGATGTAGGTGGATTAGAAA+TGG 0.507251 2.1:-34731050 None:intergenic
CGGAGGTTGTTAGGGATCCA+AGG 0.508723 2.1:+34731677 MS.gene99749:CDS
CCACAGAAGTTTCCTCAAAC+CGG 0.516850 2.1:+34732237 MS.gene99749:CDS
TTCATGAGTTGAGGAGTTTG+GGG 0.519712 2.1:+34731632 MS.gene99749:CDS
TGTAGGTGGATTAGAAATGG+TGG 0.521617 2.1:-34731047 None:intergenic
ATAAATTGGCTATCATCAGC+GGG 0.523449 2.1:-34731345 None:intergenic
TTTGAGGAAACTTCTGTGGT+TGG 0.524496 2.1:-34732233 None:intergenic
AACAGCTTCCAAAGTCCAGC+TGG 0.524566 2.1:-34732210 None:intergenic
TTGACGAAGGTGGGATGGAT+CGG 0.525161 2.1:-34731176 None:intergenic
AAATTCGGAAGAGAAGAGGA+AGG 0.533269 2.1:-34731124 None:intergenic
AGAAGGGCAATCTTGAATAA+GGG 0.535156 2.1:-34731248 None:intergenic
TTGGCTATCATCAGCGGGGA+AGG 0.535986 2.1:-34731340 None:intergenic
TTCCCCATTACAACTGCAGT+GGG 0.537140 2.1:-34731481 None:intergenic
TCATGGATTCAATGCTGTAG+TGG 0.539193 2.1:+34732286 MS.gene99749:CDS
AGGGATTGACGAAGGTGGGA+TGG 0.540489 2.1:-34731181 None:intergenic
AATAAATTGGCTATCATCAG+CGG 0.542731 2.1:-34731346 None:intergenic
TCCTAAGGATGAAATTGATA+GGG 0.543797 2.1:+34731549 MS.gene99749:CDS
GTTAAAAGTAAGCATCCAGC+TGG 0.546625 2.1:+34732195 MS.gene99749:CDS
GTGGATTGTTTGTGTGGTCA+CGG 0.552758 2.1:+34731832 MS.gene99749:CDS
ATGGTGGAACCCAACCCCAT+TGG 0.554824 2.1:-34730956 None:intergenic
ATTCCAAATCCCAAAACCCT+AGG 0.561947 2.1:-34731586 None:intergenic
GTTGCAGAGTTTGAAATGCA+GGG 0.562878 2.1:+34731744 MS.gene99749:CDS
GAAGGAAAAGAGGATGGTAA+GGG 0.566861 2.1:-34731200 None:intergenic
ATGGGGAAAAGTCAAATTGT+TGG 0.568119 2.1:+34731496 MS.gene99749:CDS
GAGGAAATTCGGAAGAGAAG+AGG 0.568672 2.1:-34731128 None:intergenic
TTGAGAGTGGATTGTTTGTG+TGG 0.573095 2.1:+34731826 MS.gene99749:CDS
TCCCAATTAAGTAGAAATTG+GGG 0.577215 2.1:-34731280 None:intergenic
AAATTCACACCTAAGTATGA+AGG 0.579741 2.1:-34731991 None:intergenic
TGCTTTGAAGGTGTTGTGGA+AGG 0.580553 2.1:+34731870 MS.gene99749:CDS
AGCTGTTGATTCATGAGTTG+AGG 0.589517 2.1:+34731623 MS.gene99749:CDS
AATTGGGGTTCCAACACAGA+AGG 0.590708 2.1:-34731265 None:intergenic
AATTCGGAAGAGAAGAGGAA+GGG 0.592899 2.1:-34731123 None:intergenic
CCGGTTTGAGGAAACTTCTG+TGG 0.592977 2.1:-34732237 None:intergenic
GGGATGGATCGGTATTGAAG+AGG 0.598546 2.1:-34731165 None:intergenic
GGAGGTTGTTAGGGATCCAA+GGG 0.599772 2.1:+34731678 MS.gene99749:CDS
TTTCCCCATTACAACTGCAG+TGG 0.603282 2.1:-34731482 None:intergenic
ACTTTATGGGAAATATCAAG+TGG 0.603369 2.1:-34731397 None:intergenic
CTTTATGGGAAATATCAAGT+GGG 0.607807 2.1:-34731396 None:intergenic
AAACCGTAAGCAATCAACCT+TGG 0.613186 2.1:-34731895 None:intergenic
GGGATGCTTTGAAGGTGTTG+TGG 0.613256 2.1:+34731866 MS.gene99749:CDS
TTCCCACTGCAGTTGTAATG+GGG 0.614215 2.1:+34731479 MS.gene99749:CDS
GGATGGTAAGGGATTGACGA+AGG 0.617740 2.1:-34731189 None:intergenic
ATTGGGGTTCCAACACAGAA+GGG 0.628360 2.1:-34731264 None:intergenic
TGATGATAGCCAATTTATTG+CGG 0.634282 2.1:+34731350 MS.gene99749:CDS
TGGTCACGGTTTGATCCGTA+GGG 0.637002 2.1:+34731846 MS.gene99749:CDS
AGGACATCATCAAACCAACT+AGG 0.642474 2.1:+34732094 MS.gene99749:CDS
AGAAGAGGAAGGGAAACCGA+AGG 0.647325 2.1:-34731113 None:intergenic
ACACCTTCAAAGCATCCCTA+CGG 0.648597 2.1:-34731861 None:intergenic
TCAAGATGTGAAGAACATGA+AGG 0.654568 2.1:+34732260 MS.gene99749:CDS
TTTCTGTAATGAGATGTAGG+TGG 0.659285 2.1:-34731061 None:intergenic
GCAGGGAGGCAATTAAAGAG+AGG 0.662756 2.1:+34731761 MS.gene99749:CDS
TTCCAGCCAAGAGGTCAGGG+CGG 0.702741 2.1:+34731657 MS.gene99749:CDS
AGGTGTTGTGGAAGGAACCA+AGG 0.704640 2.1:+34731878 MS.gene99749:CDS
GCAGAGTTTGAAATGCAGGG+AGG 0.745889 2.1:+34731747 MS.gene99749:CDS
TAAATTGGCTATCATCAGCG+GGG 0.750324 2.1:-34731344 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAGAATGTGAAAAAATATAT+GGG + chr2.1:34732151-34732170 MS.gene99749:CDS 20.0%
!!! AAAAATATATGGGAGATTTT+CGG + chr2.1:34732161-34732180 MS.gene99749:CDS 20.0%
!!! TTAATTTTCTTGTGGAATTT+GGG + chr2.1:34731524-34731543 MS.gene99749:CDS 20.0%
! AATCTTGAATAAGGGAAATT+AGG - chr2.1:34731243-34731262 None:intergenic 25.0%
! AATGTGATTGAGTATTTGAA+AGG + chr2.1:34732039-34732058 MS.gene99749:CDS 25.0%
! ATTCCCAATTAAGTAGAAAT+TGG - chr2.1:34731285-34731304 None:intergenic 25.0%
! ATTGAGTATTTGAAAGGAAA+AGG + chr2.1:34732045-34732064 MS.gene99749:CDS 25.0%
! ATTTCTACTTAATTGGGAAT+TGG + chr2.1:34731285-34731304 MS.gene99749:CDS 25.0%
! CCAGAATGTGAAAAAATATA+TGG + chr2.1:34732150-34732169 MS.gene99749:CDS 25.0%
! CCTAAAAATCTCAACTTTAT+GGG - chr2.1:34731413-34731432 None:intergenic 25.0%
! TTAATCCAAAGGATGAAATA+TGG + chr2.1:34731946-34731965 MS.gene99749:CDS 25.0%
! TTCCCAATTAAGTAGAAATT+GGG - chr2.1:34731284-34731303 None:intergenic 25.0%
! TTTCTACTTAATTGGGAATT+GGG + chr2.1:34731286-34731305 MS.gene99749:CDS 25.0%
!! AAAATATATGGGAGATTTTC+GGG + chr2.1:34732162-34732181 MS.gene99749:CDS 25.0%
!! AAATATCAAGTGGGTTTAAA+TGG - chr2.1:34731390-34731409 None:intergenic 25.0%
!! AGATTGAGTTTTTAATCCAA+AGG + chr2.1:34731935-34731954 MS.gene99749:CDS 25.0%
!! CCATAAAGTTGAGATTTTTA+GGG + chr2.1:34731411-34731430 MS.gene99749:CDS 25.0%
!! GTTAATTTTCTTGTGGAATT+TGG + chr2.1:34731523-34731542 MS.gene99749:CDS 25.0%
!! TAATTTTCTTGTGGAATTTG+GGG + chr2.1:34731525-34731544 MS.gene99749:CDS 25.0%
!!! ATTTTTTCACATTCTGGATA+AGG - chr2.1:34732147-34732166 None:intergenic 25.0%
!!! CATAAAGTTGAGATTTTTAG+GGG + chr2.1:34731412-34731431 MS.gene99749:CDS 25.0%
!!! CCATATATTTTTTCACATTC+TGG - chr2.1:34732153-34732172 None:intergenic 25.0%
AAATTCACACCTAAGTATGA+AGG - chr2.1:34731994-34732013 None:intergenic 30.0%
AACCCCAATTTCTACTTAAT+TGG + chr2.1:34731278-34731297 MS.gene99749:CDS 30.0%
AAGTGGTTCTTACAAGAATA+AGG - chr2.1:34731003-34731022 None:intergenic 30.0%
AATAAATTGGCTATCATCAG+CGG - chr2.1:34731349-34731368 None:intergenic 30.0%
ACCCCAATTTCTACTTAATT+GGG + chr2.1:34731279-34731298 MS.gene99749:CDS 30.0%
ACTTTATGGGAAATATCAAG+TGG - chr2.1:34731400-34731419 None:intergenic 30.0%
CCCTAAAAATCTCAACTTTA+TGG - chr2.1:34731414-34731433 None:intergenic 30.0%
CTTTATGGGAAATATCAAGT+GGG - chr2.1:34731399-34731418 None:intergenic 30.0%
TACTCAATCACATTGTATCT+AGG - chr2.1:34732033-34732052 None:intergenic 30.0%
TCCCAATTAAGTAGAAATTG+GGG - chr2.1:34731283-34731302 None:intergenic 30.0%
TCCTAAGGATGAAATTGATA+GGG + chr2.1:34731549-34731568 MS.gene99749:CDS 30.0%
TGATGATAGCCAATTTATTG+CGG + chr2.1:34731350-34731369 MS.gene99749:CDS 30.0%
TTCCTAAGGATGAAATTGAT+AGG + chr2.1:34731548-34731567 MS.gene99749:CDS 30.0%
TTGTAAAATCTAAGCCTAGT+TGG - chr2.1:34732111-34732130 None:intergenic 30.0%
! CCCATAAAGTTGAGATTTTT+AGG + chr2.1:34731410-34731429 MS.gene99749:CDS 30.0%
! GAAAGCTTGGATATGAATTT+TGG - chr2.1:34731316-34731335 None:intergenic 30.0%
! GCAATTAAAGAGAGGATTTT+TGG + chr2.1:34731769-34731788 MS.gene99749:CDS 30.0%
! GTTGTTATTAGTTGAAGAAG+TGG - chr2.1:34731020-34731039 None:intergenic 30.0%
! GTTTTTCTGTGGAATTTTGA+AGG - chr2.1:34731221-34731240 None:intergenic 30.0%
! TGTTTTCTGTAATGAGATGT+AGG - chr2.1:34731067-34731086 None:intergenic 30.0%
! TTCTGATGATGTTGTGATTA+GGG + chr2.1:34731444-34731463 MS.gene99749:CDS 30.0%
! TTTCTGATGATGTTGTGATT+AGG + chr2.1:34731443-34731462 MS.gene99749:CDS 30.0%
!! AATTTTGAAGGAAAAGAGGA+TGG - chr2.1:34731209-34731228 None:intergenic 30.0%
!! TTGAGATTTTTAGGGGTTTA+GGG + chr2.1:34731419-34731438 MS.gene99749:CDS 30.0%
!!! GTTGTTTCTTTTTCTGACTA+TGG - chr2.1:34730978-34730997 None:intergenic 30.0%
ACCCTATCAATTTCATCCTT+AGG - chr2.1:34731553-34731572 None:intergenic 35.0%
AGAAGGGCAATCTTGAATAA+GGG - chr2.1:34731251-34731270 None:intergenic 35.0%
ATAAATTGGCTATCATCAGC+GGG - chr2.1:34731348-34731367 None:intergenic 35.0%
ATGGGGAAAAGTCAAATTGT+TGG + chr2.1:34731496-34731515 MS.gene99749:CDS 35.0%
CAACACCATATTTCATCCTT+TGG - chr2.1:34731954-34731973 None:intergenic 35.0%
CATGAAGTTCCTTCATACTT+AGG + chr2.1:34731982-34732001 MS.gene99749:CDS 35.0%
GGTTGTTAGATTGTTTCCTA+GGG + chr2.1:34731570-34731589 MS.gene99749:CDS 35.0%
TCAAGATGTGAAGAACATGA+AGG + chr2.1:34732260-34732279 MS.gene99749:CDS 35.0%
TGTAGAACACCGCAATAAAT+TGG - chr2.1:34731362-34731381 None:intergenic 35.0%
TTTCTGTAATGAGATGTAGG+TGG - chr2.1:34731064-34731083 None:intergenic 35.0%
! AGATTGTTTCCTAGGGTTTT+GGG + chr2.1:34731577-34731596 MS.gene99749:CDS 35.0%
! AGGGAAATTAGGTTTTTCTG+TGG - chr2.1:34731232-34731251 None:intergenic 35.0%
! ATTCATGAGTTGAGGAGTTT+GGG + chr2.1:34731631-34731650 MS.gene99749:CDS 35.0%
! ATTGAAGAGGAAGTGGTTTT+TGG - chr2.1:34731155-34731174 None:intergenic 35.0%
! GTTTCTTTTTCTGACTATGG+TGG - chr2.1:34730975-34730994 None:intergenic 35.0%
! TAGATTGTTTCCTAGGGTTT+TGG + chr2.1:34731576-34731595 MS.gene99749:CDS 35.0%
! TGAAAGGAAAAGGTGCAATT+GGG + chr2.1:34732055-34732074 MS.gene99749:CDS 35.0%
! TGATGTTGTGATTAGGGTTT+TGG + chr2.1:34731450-34731469 MS.gene99749:CDS 35.0%
! TTGAAAGGAAAAGGTGCAAT+TGG + chr2.1:34732054-34732073 MS.gene99749:CDS 35.0%
!! AAAGAGAGGATTTTTGGAGA+GGG + chr2.1:34731775-34731794 MS.gene99749:CDS 35.0%
!! AGATGTAGGTGGATTAGAAA+TGG - chr2.1:34731053-34731072 None:intergenic 35.0%
!! GTGGAATTTTGAAGGAAAAG+AGG - chr2.1:34731213-34731232 None:intergenic 35.0%
!! GTTGAGATTTTTAGGGGTTT+AGG + chr2.1:34731418-34731437 MS.gene99749:CDS 35.0%
!! TAAAGAGAGGATTTTTGGAG+AGG + chr2.1:34731774-34731793 MS.gene99749:CDS 35.0%
!! TGGTTTTTGGAGAGGAAATT+CGG - chr2.1:34731142-34731161 None:intergenic 35.0%
!!! TGGTGTCGTTAATTTTCTTG+TGG + chr2.1:34731516-34731535 MS.gene99749:CDS 35.0%
!!! TTTTGAAGTGAAGTTGAGAG+TGG + chr2.1:34731813-34731832 MS.gene99749:CDS 35.0%
AAACCGTAAGCAATCAACCT+TGG - chr2.1:34731898-34731917 None:intergenic 40.0%
AAATTCGGAAGAGAAGAGGA+AGG - chr2.1:34731127-34731146 None:intergenic 40.0%
AATTCGGAAGAGAAGAGGAA+GGG - chr2.1:34731126-34731145 None:intergenic 40.0%
AGGACATCATCAAACCAACT+AGG + chr2.1:34732094-34732113 MS.gene99749:CDS 40.0%
ATTCCAAATCCCAAAACCCT+AGG - chr2.1:34731589-34731608 None:intergenic 40.0%
ATTTCCCACTGCAGTTGTAA+TGG + chr2.1:34731477-34731496 MS.gene99749:CDS 40.0%
CAGAAGGGCAATCTTGAATA+AGG - chr2.1:34731252-34731271 None:intergenic 40.0%
CATGTTCTTCACATCTTGAC+CGG - chr2.1:34732259-34732278 None:intergenic 40.0%
GAAGAACATGAAGGCTTTCA+TGG + chr2.1:34732269-34732288 MS.gene99749:CDS 40.0%
GAAGGAAAAGAGGATGGTAA+GGG - chr2.1:34731203-34731222 None:intergenic 40.0%
GCTAATCTCTTCCAATCCTT+CGG + chr2.1:34731097-34731116 MS.gene99749:CDS 40.0%
GGGTTGTTAGATTGTTTCCT+AGG + chr2.1:34731569-34731588 MS.gene99749:CDS 40.0%
GTTAAAAGTAAGCATCCAGC+TGG + chr2.1:34732195-34732214 MS.gene99749:CDS 40.0%
TAAATTGGCTATCATCAGCG+GGG - chr2.1:34731347-34731366 None:intergenic 40.0%
TGAAGGAAAAGAGGATGGTA+AGG - chr2.1:34731204-34731223 None:intergenic 40.0%
TTTCCCACTGCAGTTGTAAT+GGG + chr2.1:34731478-34731497 MS.gene99749:CDS 40.0%
TTTGAGGAAACTTCTGTGGT+TGG - chr2.1:34732236-34732255 None:intergenic 40.0%
! AAGGTTGATTGCTTACGGTT+TGG + chr2.1:34731897-34731916 MS.gene99749:CDS 40.0%
! GAACCAAGGTTGATTGCTTA+CGG + chr2.1:34731892-34731911 MS.gene99749:CDS 40.0%
! GATTCATGAGTTGAGGAGTT+TGG + chr2.1:34731630-34731649 MS.gene99749:CDS 40.0%
! GTTGCAGAGTTTGAAATGCA+GGG + chr2.1:34731744-34731763 MS.gene99749:CDS 40.0%
! TGGAGATTGGGGAATTTTCA+CGG + chr2.1:34731710-34731729 MS.gene99749:CDS 40.0%
! TGGGATTTGGAATTGAGGAT+AGG + chr2.1:34731596-34731615 MS.gene99749:CDS 40.0%
! TGGGTTTGAAGTTGGATTGA+AGG + chr2.1:34732074-34732093 MS.gene99749:CDS 40.0%
! TGTTGCAGAGTTTGAAATGC+AGG + chr2.1:34731743-34731762 MS.gene99749:CDS 40.0%
! TTCATGAGTTGAGGAGTTTG+GGG + chr2.1:34731632-34731651 MS.gene99749:CDS 40.0%
!! AGCTGTTGATTCATGAGTTG+AGG + chr2.1:34731623-34731642 MS.gene99749:CDS 40.0%
!! TGTAGGTGGATTAGAAATGG+TGG - chr2.1:34731050-34731069 None:intergenic 40.0%
!! TGTTGTGATTAGGGTTTTGG+AGG + chr2.1:34731453-34731472 MS.gene99749:CDS 40.0%
!! TTGAGAGTGGATTGTTTGTG+TGG + chr2.1:34731826-34731845 MS.gene99749:CDS 40.0%
!! TTTTGGAGAGGGTTTGGTTA+GGG + chr2.1:34731786-34731805 MS.gene99749:CDS 40.0%
!! TTTTTGGAGAGGGTTTGGTT+AGG + chr2.1:34731785-34731804 MS.gene99749:CDS 40.0%
!!! ATATGGGAGATTTTCGGGTA+AGG + chr2.1:34732167-34732186 MS.gene99749:CDS 40.0%
!!! GGTTTTGGGATTTGGAATTG+AGG + chr2.1:34731591-34731610 MS.gene99749:CDS 40.0%
!!! TTTCCTAGGGTTTTGGGATT+TGG + chr2.1:34731583-34731602 MS.gene99749:CDS 40.0%
AATTGGGGTTCCAACACAGA+AGG - chr2.1:34731268-34731287 None:intergenic 45.0%
ATCTCCATTCCCAAAACCCT+TGG - chr2.1:34731697-34731716 None:intergenic 45.0%
ATTGGGGTTCCAACACAGAA+GGG - chr2.1:34731267-34731286 None:intergenic 45.0%
CCACAGAAGTTTCCTCAAAC+CGG + chr2.1:34732237-34732256 MS.gene99749:CDS 45.0%
GAGGAAATTCGGAAGAGAAG+AGG - chr2.1:34731131-34731150 None:intergenic 45.0%
TCAAGATTGCCCTTCTGTGT+TGG + chr2.1:34731255-34731274 MS.gene99749:CDS 45.0%
TTCCCACTGCAGTTGTAATG+GGG + chr2.1:34731479-34731498 MS.gene99749:CDS 45.0%
TTCCCCATTACAACTGCAGT+GGG - chr2.1:34731484-34731503 None:intergenic 45.0%
TTTCCCCATTACAACTGCAG+TGG - chr2.1:34731485-34731504 None:intergenic 45.0%
! ACACCTTCAAAGCATCCCTA+CGG - chr2.1:34731864-34731883 None:intergenic 45.0%
! GATCGGTATTGAAGAGGAAG+TGG - chr2.1:34731162-34731181 None:intergenic 45.0%
! GGTGCAATTGGGTTTGAAGT+TGG + chr2.1:34732066-34732085 MS.gene99749:CDS 45.0%
! GTGGATTGTTTGTGTGGTCA+CGG + chr2.1:34731832-34731851 MS.gene99749:CDS 45.0%
! GTTAGGGATCCAAGGGTTTT+GGG + chr2.1:34731685-34731704 MS.gene99749:CDS 45.0%
! GTTGATTGCTTACGGTTTGG+AGG + chr2.1:34731900-34731919 MS.gene99749:CDS 45.0%
! TGTGGAATTTGGGGTTCCTA+AGG + chr2.1:34731534-34731553 MS.gene99749:CDS 45.0%
! TGTTAGGGATCCAAGGGTTT+TGG + chr2.1:34731684-34731703 MS.gene99749:CDS 45.0%
!! AGAGGAAGTGGTTTTTGGAG+AGG - chr2.1:34731150-34731169 None:intergenic 45.0%
!! GAGGATTTTTGGAGAGGGTT+TGG + chr2.1:34731780-34731799 MS.gene99749:CDS 45.0%
!! TGCTTTGAAGGTGTTGTGGA+AGG + chr2.1:34731870-34731889 MS.gene99749:CDS 45.0%
!! TTCACATCTTGACCGGTTTG+AGG - chr2.1:34732252-34732271 None:intergenic 45.0%
!!! AGGGTTTTGGGAATGGAGAT+TGG + chr2.1:34731697-34731716 MS.gene99749:CDS 45.0%
!!! GGGTTTTGGGAATGGAGATT+GGG + chr2.1:34731698-34731717 MS.gene99749:CDS 45.0%
!!! GGTTTTGGGAATGGAGATTG+GGG + chr2.1:34731699-34731718 MS.gene99749:CDS 45.0%
AACAGCTTCCAAAGTCCAGC+TGG - chr2.1:34732213-34732232 None:intergenic 50.0%
AGAAGAGGAAGGGAAACCGA+AGG - chr2.1:34731116-34731135 None:intergenic 50.0%
AGGAAGGGAAACCGAAGGAT+TGG - chr2.1:34731111-34731130 None:intergenic 50.0%
CGGGGAAGGATTAGAAAGCT+TGG - chr2.1:34731329-34731348 None:intergenic 50.0%
GATCCGTAGGGATGCTTTGA+AGG + chr2.1:34731858-34731877 MS.gene99749:CDS 50.0%
GCAGGGAGGCAATTAAAGAG+AGG + chr2.1:34731761-34731780 MS.gene99749:CDS 50.0%
GGAGGTTGTTAGGGATCCAA+GGG + chr2.1:34731678-34731697 MS.gene99749:CDS 50.0%
GGATGGTAAGGGATTGACGA+AGG - chr2.1:34731192-34731211 None:intergenic 50.0%
GGTAAGGGATTGACGAAGGT+GGG - chr2.1:34731188-34731207 None:intergenic 50.0%
GTAAGCATCCAGCTGGACTT+TGG + chr2.1:34732202-34732221 MS.gene99749:CDS 50.0%
TGGTAAGGGATTGACGAAGG+TGG - chr2.1:34731189-34731208 None:intergenic 50.0%
TGGTCACGGTTTGATCCGTA+GGG + chr2.1:34731846-34731865 MS.gene99749:CDS 50.0%
TTGACGAAGGTGGGATGGAT+CGG - chr2.1:34731179-34731198 None:intergenic 50.0%
! AGGTGTTGTGGAAGGAACCA+AGG + chr2.1:34731878-34731897 MS.gene99749:CDS 50.0%
! CCGGTTTGAGGAAACTTCTG+TGG - chr2.1:34732240-34732259 None:intergenic 50.0%
! GCAGAGTTTGAAATGCAGGG+AGG + chr2.1:34731747-34731766 MS.gene99749:CDS 50.0%
! GGGATGGATCGGTATTGAAG+AGG - chr2.1:34731168-34731187 None:intergenic 50.0%
! GTTTTCCAGCCAAGAGGTCA+GGG + chr2.1:34731654-34731673 MS.gene99749:CDS 50.0%
!! GGGATGCTTTGAAGGTGTTG+TGG + chr2.1:34731866-34731885 MS.gene99749:CDS 50.0%
!! TTTGGGGTTTTCCAGCCAAG+AGG + chr2.1:34731648-34731667 MS.gene99749:CDS 50.0%
!!! GGATCCAAGGGTTTTGGGAA+TGG + chr2.1:34731690-34731709 MS.gene99749:CDS 50.0%
AGGGATTGACGAAGGTGGGA+TGG - chr2.1:34731184-34731203 None:intergenic 55.0%
CGGAGGTTGTTAGGGATCCA+AGG + chr2.1:34731677-34731696 MS.gene99749:CDS 55.0%
GTGGTCACGGTTTGATCCGT+AGG + chr2.1:34731845-34731864 MS.gene99749:CDS 55.0%
TTGGCTATCATCAGCGGGGA+AGG - chr2.1:34731343-34731362 None:intergenic 55.0%
! GGTTTTCCAGCCAAGAGGTC+AGG + chr2.1:34731653-34731672 MS.gene99749:CDS 55.0%
AGGTCAGGGCGGAGGTTGTT+AGG + chr2.1:34731668-34731687 MS.gene99749:CDS 60.0%
GGTCAGGGCGGAGGTTGTTA+GGG + chr2.1:34731669-34731688 MS.gene99749:CDS 60.0%
TTCCAGCCAAGAGGTCAGGG+CGG + chr2.1:34731657-34731676 MS.gene99749:CDS 60.0%
CAACCTCCGCCCTGACCTCT+TGG - chr2.1:34731666-34731685 None:intergenic 65.0%
CAGCCAAGAGGTCAGGGCGG+AGG + chr2.1:34731660-34731679 MS.gene99749:CDS 70.0%
CTCCGCCCTGACCTCTTGGC+TGG - chr2.1:34731662-34731681 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 34730959 34732305 34730959 ID=MS.gene99749
chr2.1 mRNA 34730959 34732305 34730959 ID=MS.gene99749.t1;Parent=MS.gene99749
chr2.1 exon 34730959 34732305 34730959 ID=MS.gene99749.t1.exon1;Parent=MS.gene99749.t1
chr2.1 CDS 34730959 34732305 34730959 ID=cds.MS.gene99749.t1;Parent=MS.gene99749.t1
Gene Sequence

>MS.gene99749

ATGGGGTTGGGTTCCACCATAGTCAGAAAAAGAAACAACGTCCTTATTCTTGTAAGAACCACTTCTTCAACTAATAACAACTACTTCTCCACCATTTCTAATCCACCTACATCTCATTACAGAAAACAAATCGCACTCGCTAATCTCTTCCAATCCTTCGGTTTCCCTTCCTCTTCTCTTCCGAATTTCCTCTCCAAAAACCACTTCCTCTTCAATACCGATCCATCCCACCTTCGTCAATCCCTTACCATCCTCTTTTCCTTCAAAATTCCACAGAAAAACCTAATTTCCCTTATTCAAGATTGCCCTTCTGTGTTGGAACCCCAATTTCTACTTAATTGGGAATTGGGTTTTCCAAAATTCATATCCAAGCTTTCTAATCCTTCCCCGCTGATGATAGCCAATTTATTGCGGTGTTCTACAACTTTCCATTTAAACCCACTTGATATTTCCCATAAAGTTGAGATTTTTAGGGGTTTAGGGTTTTCTGATGATGTTGTGATTAGGGTTTTGGAGGAATTTCCCACTGCAGTTGTAATGGGGAAAAGTCAAATTGTTGGTGTCGTTAATTTTCTTGTGGAATTTGGGGTTCCTAAGGATGAAATTGATAGGGTTGTTAGATTGTTTCCTAGGGTTTTGGGATTTGGAATTGAGGATAGGTTGAAGCTGTTGATTCATGAGTTGAGGAGTTTGGGGTTTTCCAGCCAAGAGGTCAGGGCGGAGGTTGTTAGGGATCCAAGGGTTTTGGGAATGGAGATTGGGGAATTTTCACGGTATTTGAAGTTGTTGCAGAGTTTGAAATGCAGGGAGGCAATTAAAGAGAGGATTTTTGGAGAGGGTTTGGTTAGGGCTTGTTTTGAAGTGAAGTTGAGAGTGGATTGTTTGTGTGGTCACGGTTTGATCCGTAGGGATGCTTTGAAGGTGTTGTGGAAGGAACCAAGGTTGATTGCTTACGGTTTGGAGGATATTGAAAAGAAGATTGAGTTTTTAATCCAAAGGATGAAATATGGTGTTGATTGTTTGCATGAAGTTCCTTCATACTTAGGTGTGAATTTTGAAAAACAGATTGTTCCTAGATACAATGTGATTGAGTATTTGAAAGGAAAAGGTGCAATTGGGTTTGAAGTTGGATTGAAGGACATCATCAAACCAACTAGGCTTAGATTTTACAATCTTTACGTTAAGCCTTATCCAGAATGTGAAAAAATATATGGGAGATTTTCGGGTAAGGTCGAAGTTAAAAGTAAGCATCCAGCTGGACTTTGGAAGCTGTTCCAACCACAGAAGTTTCCTCAAACCGGTCAAGATGTGAAGAACATGAAGGCTTTCATGGATTCAATGCTGTAG
Protein sequence

>MS.gene99749.t1

MGLGSTIVRKRNNVLILVRTTSSTNNNYFSTISNPPTSHYRKQIALANLFQSFGFPSSSLPNFLSKNHFLFNTDPSHLRQSLTILFSFKIPQKNLISLIQDCPSVLEPQFLLNWELGFPKFISKLSNPSPLMIANLLRCSTTFHLNPLDISHKVEIFRGLGFSDDVVIRVLEEFPTAVVMGKSQIVGVVNFLVEFGVPKDEIDRVVRLFPRVLGFGIEDRLKLLIHELRSLGFSSQEVRAEVVRDPRVLGMEIGEFSRYLKLLQSLKCREAIKERIFGEGLVRACFEVKLRVDCLCGHGLIRRDALKVLWKEPRLIAYGLEDIEKKIEFLIQRMKYGVDCLHEVPSYLGVNFEKQIVPRYNVIEYLKGKGAIGFEVGLKDIIKPTRLRFYNLYVKPYPECEKIYGRFSGKVEVKSKHPAGLWKLFQPQKFPQTGQDVKNMKAFMDSML