AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99765

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99765.t1 MTR_2g061450 91.264 435 35 2 14 448 1 432 0.0 800
MS.gene99765.t1 MTR_2g060620 76.190 126 30 0 318 443 61 186 2.24e-60 196
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99765.t1 AT1G74120 49.436 443 220 2 6 448 7 445 6.87e-154 444
MS.gene99765.t1 AT5G54180 29.279 222 151 5 153 370 253 472 6.64e-16 80.1

Find 106 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACCTCAATTTCTACTTAAT+TGG 0.095296 2.4:-32700449 MS.gene99765:CDS
GAAAGCTTGGATATGAATTT+TGG 0.127070 2.4:+32700414 None:intergenic
TGTCAGGGATCCAAGGATTT+TGG 0.150914 2.4:-32700043 MS.gene99765:CDS
CATGTTCTTCACATCTTTAC+TGG 0.159159 2.4:+32699471 None:intergenic
TAGATTGTTTCCTAGGGTTT+TGG 0.199228 2.4:-32700151 MS.gene99765:CDS
AAATTTCAAGTGGGTTTAAA+TGG 0.215029 2.4:+32700340 None:intergenic
AATCTTGAAGAAGTGAAATT+AGG 0.248181 2.4:+32700487 None:intergenic
ATTTCTACTTAATTGGGAAT+TGG 0.257535 2.4:-32700442 MS.gene99765:CDS
CAACACCATATTTCATCCTT+TGG 0.306291 2.4:+32699776 None:intergenic
AAATTCACACCCATGTATTC+AGG 0.313425 2.4:+32699736 None:intergenic
AGGTCAGGGCGGAGGTTGTC+AGG 0.314844 2.4:-32700059 MS.gene99765:CDS
TTAATCCAAAGGATGAAATA+TGG 0.315058 2.4:-32699781 MS.gene99765:CDS
TTGAAAGGAAAAGGTGCAAT+TGG 0.315697 2.4:-32699673 MS.gene99765:CDS
ACCTCAATTTCTACTTAATT+GGG 0.327209 2.4:-32700448 MS.gene99765:CDS
TTTCTACTTAATTGGGAATT+GGG 0.329864 2.4:-32700441 MS.gene99765:CDS
TTTCCCATTGCAGTTGTAAT+GGG 0.333921 2.4:-32700249 MS.gene99765:CDS
AAGTGGTTCTTACAAGAATA+AGG 0.352405 2.4:+32700727 None:intergenic
GGGTTGTTAGATTGTTTCCT+AGG 0.353615 2.4:-32700158 MS.gene99765:CDS
GAAGGAAAAGAGGGTTGTTA+AGG 0.369880 2.4:+32700527 None:intergenic
GAAGAACATGAAGGCTTTCA+TGG 0.374988 2.4:-32699458 MS.gene99765:CDS
CACAATCTTTCCAATGGGGT+TGG 0.394153 2.4:-32700781 None:intergenic
AAGGATGATTGCTTACGGTT+TGG 0.397737 2.4:-32699830 MS.gene99765:CDS
GGTGCAATTGGGTTTGAAGT+TGG 0.399622 2.4:-32699661 MS.gene99765:CDS
TTCACATCTTTACTGGTTTG+AGG 0.401092 2.4:+32699478 None:intergenic
TCACATCTTTACTGGTTTGA+GGG 0.406910 2.4:+32699479 None:intergenic
GATTCGTAGGGATGCTTTGA+AGG 0.407568 2.4:-32699869 MS.gene99765:CDS
GAAAGCATCCAGCTGGACTT+TGG 0.410449 2.4:-32699525 MS.gene99765:CDS
ACAAGTTGGCGATCATCAAC+GGG 0.415975 2.4:+32700382 None:intergenic
AGGAAGGGAAACCGAAGGTT+TGG 0.417132 2.4:+32700619 None:intergenic
ATTTCCCATTGCAGTTGTAA+TGG 0.419491 2.4:-32700250 MS.gene99765:CDS
TTCCTAAGGATGAGATTGAT+AGG 0.422720 2.4:-32700179 MS.gene99765:CDS
ACAATCTTTCCAATGGGGTT+GGG 0.423298 2.4:-32700780 None:intergenic
AAACCACTTCCTCTTCAATT+CGG 0.423347 2.4:-32700571 MS.gene99765:CDS
CATTGTATCTAGGAACAATC+TGG 0.424186 2.4:+32699707 None:intergenic
TGAAAGGAAAAGGTGCAATT+GGG 0.424464 2.4:-32699672 MS.gene99765:CDS
TGTTGCAGAGTTTGAAATGC+AGG 0.431814 2.4:-32699984 MS.gene99765:CDS
TTATAAAATCTAAGTCTAGT+TGG 0.433063 2.4:+32699619 None:intergenic
TCAAGATTGCCCTTCTGTGT+TGG 0.433382 2.4:-32700472 MS.gene99765:CDS
TTACTGGTTTGAGGGAACTT+TGG 0.436445 2.4:+32699487 None:intergenic
GCTAATCTCTTCCAAACCTT+CGG 0.450033 2.4:-32700630 MS.gene99765:CDS
ATCTCCATTCCCAAAATCCT+TGG 0.454905 2.4:+32700033 None:intergenic
TACTCAATCACATTGTATCT+AGG 0.456654 2.4:+32699697 None:intergenic
GCGGTCACGGTTTGATTCGT+AGG 0.460595 2.4:-32699882 MS.gene99765:CDS
GATGATTGCTTACGGTTTGG+AGG 0.460844 2.4:-32699827 MS.gene99765:CDS
TCCCAATTAAGTAGAAATTG+AGG 0.461386 2.4:+32700447 None:intergenic
TTGTTAAGGATTGGCGAAGG+TGG 0.462969 2.4:+32700541 None:intergenic
GTTGTTATTAGTTGAAGAAG+TGG 0.465877 2.4:+32700710 None:intergenic
CGGGGAAGCATTAGAAAGCT+TGG 0.465957 2.4:+32700401 None:intergenic
AAATGGGGAAGAGAAGAGGA+AGG 0.466733 2.4:+32700603 None:intergenic
TGTTAAGGATTGGCGAAGGT+GGG 0.467753 2.4:+32700542 None:intergenic
TATGGAATTTGGGGTTCCTA+AGG 0.471062 2.4:-32700193 MS.gene99765:CDS
ATTGAGTATTTGAAAGGAAA+AGG 0.475963 2.4:-32699682 MS.gene99765:CDS
CAGTCGAGAGGTCAGGGCGG+AGG 0.476069 2.4:-32700067 MS.gene99765:CDS
TTTGAGGGAACTTTGGTGGT+TGG 0.477860 2.4:+32699494 None:intergenic
AAAAGAGGGTTGTTAAGGAT+TGG 0.487510 2.4:+32700532 None:intergenic
GTGGATTGTTTGTGCGGTCA+CGG 0.490001 2.4:-32699895 MS.gene99765:CDS
CTGGTTTGAGGGAACTTTGG+TGG 0.495315 2.4:+32699490 None:intergenic
GGTTGTTAGATTGTTTCCTA+GGG 0.496449 2.4:-32700157 MS.gene99765:CDS
AATGTGATTGAGTATTTGAA+AGG 0.505696 2.4:-32699688 MS.gene99765:CDS
AGATGTAGGTGGATTAGAAA+TGG 0.507251 2.4:+32700677 None:intergenic
ACCCTATCAATCTCATCCTT+AGG 0.512362 2.4:+32700177 None:intergenic
AACAAGTTGGCGATCATCAA+CGG 0.516565 2.4:+32700381 None:intergenic
TGTAGGTGGATTAGAAATGG+TGG 0.521617 2.4:+32700680 None:intergenic
AACATCTTCCAAAGTCCAGC+TGG 0.524566 2.4:+32699517 None:intergenic
GATCCGAATTGAAGAGGAAG+TGG 0.526858 2.4:+32700568 None:intergenic
GAACCAAGGATGATTGCTTA+CGG 0.528687 2.4:-32699835 MS.gene99765:CDS
GCCGTTGATTCATGAGTTGA+GGG 0.529192 2.4:-32700103 MS.gene99765:CDS
GCATGAAGTTCCTGAATACA+TGG 0.531558 2.4:-32699746 MS.gene99765:CDS
AGCCGTTGATTCATGAGTTG+AGG 0.532065 2.4:-32700104 MS.gene99765:CDS
TCCTAAGGATGAGATTGATA+GGG 0.538639 2.4:-32700178 MS.gene99765:CDS
CTCCGCCCTGACCTCTCGAC+TGG 0.540014 2.4:+32700068 None:intergenic
GAGGAAATGGGGAAGAGAAG+AGG 0.540975 2.4:+32700599 None:intergenic
AAACCGTAAGCAATCATCCT+TGG 0.541022 2.4:+32699832 None:intergenic
GTTAAAAGAAAGCATCCAGC+TGG 0.543247 2.4:-32699532 MS.gene99765:CDS
CGGAGGTTGTCAGGGATCCA+AGG 0.544470 2.4:-32700050 MS.gene99765:CDS
AAGGATTGGCGAAGGTGGGA+TGG 0.547823 2.4:+32700546 None:intergenic
CGGTCACGGTTTGATTCGTA+GGG 0.549755 2.4:-32699881 MS.gene99765:CDS
ATGGTGGAACCCAACCCCAT+TGG 0.554824 2.4:+32700771 None:intergenic
CGTTGATTCATGAGTTGAGG+GGG 0.559779 2.4:-32700101 MS.gene99765:CDS
GTTGCAGAGTTTGAAATGCA+GGG 0.562878 2.4:-32699983 MS.gene99765:CDS
ATACAAAATCCCAAAACCCT+AGG 0.565652 2.4:+32700141 None:intergenic
CCCCTCAACTCATGAATCAA+CGG 0.566111 2.4:+32700102 None:intergenic
GAAGGCTTTCATGGACTCGT+TGG 0.570328 2.4:-32699449 MS.gene99765:CDS
TCCCATTGCAGTTGTAATGG+GGG 0.579132 2.4:-32700247 MS.gene99765:CDS
TGCTTTGAAGGTGTTGTGGA+AGG 0.580553 2.4:-32699857 MS.gene99765:CDS
GGTCAGGGCGGAGGTTGTCA+GGG 0.584107 2.4:-32700058 MS.gene99765:CDS
TTCCCATTGCAGTTGTAATG+GGG 0.586307 2.4:-32700248 MS.gene99765:CDS
AATTGAGGTTCCAACACAGA+AGG 0.588412 2.4:+32700462 None:intergenic
AATGGGGAAGAGAAGAGGAA+GGG 0.597700 2.4:+32700604 None:intergenic
TGTAGAACAACTCAACAAGT+TGG 0.602305 2.4:+32700368 None:intergenic
ATTGAGGTTCCAACACAGAA+GGG 0.606343 2.4:+32700463 None:intergenic
GGGTTGTTAAGGATTGGCGA+AGG 0.612411 2.4:+32700538 None:intergenic
GGGATGCTTTGAAGGTGTTG+TGG 0.613256 2.4:-32699861 MS.gene99765:CDS
TAAAGATGTGAAGAACATGA+AGG 0.617044 2.4:-32699467 MS.gene99765:CDS
AGAAGAGGAAGGGAAACCGA+AGG 0.621331 2.4:+32700614 None:intergenic
GGGATGGATCCGAATTGAAG+AGG 0.622695 2.4:+32700562 None:intergenic
CCGTTGATTCATGAGTTGAG+GGG 0.629124 2.4:-32700102 MS.gene99765:CDS
ACTTTACGGGAAATTTCAAG+TGG 0.630860 2.4:+32700330 None:intergenic
CTGAGAGTGGATTGTTTGTG+CGG 0.636549 2.4:-32699901 MS.gene99765:CDS
CTTTACGGGAAATTTCAAGT+GGG 0.640015 2.4:+32700331 None:intergenic
TTTCTGTAATGAGATGTAGG+TGG 0.659285 2.4:+32700666 None:intergenic
CATGAAGTTCCTGAATACAT+GGG 0.707620 2.4:-32699745 MS.gene99765:CDS
AGGTGTTGTGGAAGGAACCA+AGG 0.715844 2.4:-32699849 MS.gene99765:CDS
TTCCAGTCGAGAGGTCAGGG+CGG 0.718711 2.4:-32700070 MS.gene99765:CDS
GCAGAGTTTGAAATGCAGGG+AGG 0.745889 2.4:-32699980 MS.gene99765:CDS
CAAGTTGGCGATCATCAACG+GGG 0.759642 2.4:+32700383 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAATTTTCTTATGGAATTT+GGG - chr2.4:32700009-32700028 MS.gene99765:CDS 15.0%
!! TTATAAAATCTAAGTCTAGT+TGG + chr2.4:32700596-32700615 None:intergenic 20.0%
!!! AATTAAAGAGAAGATTTTTG+GGG - chr2.4:32700256-32700275 MS.gene99765:CDS 20.0%
!!! CAATTAAAGAGAAGATTTTT+GGG - chr2.4:32700255-32700274 MS.gene99765:CDS 20.0%
!!! CATATATTTTTTCACATTCA+GGG + chr2.4:32700637-32700656 None:intergenic 20.0%
!!! GTTAATTTTCTTATGGAATT+TGG - chr2.4:32700008-32700027 MS.gene99765:CDS 20.0%
!!! TAATTTTCTTATGGAATTTG+GGG - chr2.4:32700010-32700029 MS.gene99765:CDS 20.0%
! AACCTCAATTTCTACTTAAT+TGG - chr2.4:32699763-32699782 MS.gene99765:CDS 25.0%
! AATCTTGAAGAAGTGAAATT+AGG + chr2.4:32699728-32699747 None:intergenic 25.0%
! AATGTGATTGAGTATTTGAA+AGG - chr2.4:32700524-32700543 MS.gene99765:CDS 25.0%
! ACCTCAATTTCTACTTAATT+GGG - chr2.4:32699764-32699783 MS.gene99765:CDS 25.0%
! ATTGAGTATTTGAAAGGAAA+AGG - chr2.4:32700530-32700549 MS.gene99765:CDS 25.0%
! ATTTCTACTTAATTGGGAAT+TGG - chr2.4:32699770-32699789 MS.gene99765:CDS 25.0%
! CCCTAAAAATTTCAACTTTA+CGG + chr2.4:32699899-32699918 None:intergenic 25.0%
! CCTAAAAATTTCAACTTTAC+GGG + chr2.4:32699898-32699917 None:intergenic 25.0%
! CCTGAATGTGAAAAAATATA+TGG - chr2.4:32700635-32700654 MS.gene99765:CDS 25.0%
! TTAATCCAAAGGATGAAATA+TGG - chr2.4:32700431-32700450 MS.gene99765:CDS 25.0%
! TTTCTACTTAATTGGGAATT+GGG - chr2.4:32699771-32699790 MS.gene99765:CDS 25.0%
!! AAATTTCAAGTGGGTTTAAA+TGG + chr2.4:32699875-32699894 None:intergenic 25.0%
!! AGATTGAGTTTTTAATCCAA+AGG - chr2.4:32700420-32700439 MS.gene99765:CDS 25.0%
!! CCGTAAAGTTGAAATTTTTA+GGG - chr2.4:32699896-32699915 MS.gene99765:CDS 25.0%
!! GCAATTAAAGAGAAGATTTT+TGG - chr2.4:32700254-32700273 MS.gene99765:CDS 25.0%
!!! CCATATATTTTTTCACATTC+AGG + chr2.4:32700638-32700657 None:intergenic 25.0%
!!! CGTAAAGTTGAAATTTTTAG+GGG - chr2.4:32699897-32699916 MS.gene99765:CDS 25.0%
!!! TTCTGATGATGTTTTGTTAA+GGG - chr2.4:32699929-32699948 MS.gene99765:CDS 25.0%
!!! TTTCTGATGATGTTTTGTTA+AGG - chr2.4:32699928-32699947 MS.gene99765:CDS 25.0%
AAGTGGTTCTTACAAGAATA+AGG + chr2.4:32699488-32699507 None:intergenic 30.0%
TAAAGATGTGAAGAACATGA+AGG - chr2.4:32700745-32700764 MS.gene99765:CDS 30.0%
TACTCAATCACATTGTATCT+AGG + chr2.4:32700518-32700537 None:intergenic 30.0%
TCCCAATTAAGTAGAAATTG+AGG + chr2.4:32699768-32699787 None:intergenic 30.0%
! AGTGAAATTAGGTTTTTCTG+TGG + chr2.4:32699717-32699736 None:intergenic 30.0%
! ATATGGCAGATTTTCAGAAA+AGG - chr2.4:32700652-32700671 MS.gene99765:CDS 30.0%
! CCCGTAAAGTTGAAATTTTT+AGG - chr2.4:32699895-32699914 MS.gene99765:CDS 30.0%
! GAAAGCTTGGATATGAATTT+TGG + chr2.4:32699801-32699820 None:intergenic 30.0%
! GTTGTTATTAGTTGAAGAAG+TGG + chr2.4:32699505-32699524 None:intergenic 30.0%
! GTTTTTCTGTGGAATTTTGA+AGG + chr2.4:32699706-32699725 None:intergenic 30.0%
! TGTTTTCTGTAATGAGATGT+AGG + chr2.4:32699552-32699571 None:intergenic 30.0%
!! GTTGAAATTTTTAGGGGTTT+AGG - chr2.4:32699903-32699922 MS.gene99765:CDS 30.0%
!! TGATGTTTTGTTAAGGGTTT+TGG - chr2.4:32699935-32699954 MS.gene99765:CDS 30.0%
!! TGGAATTTTGAAGGAAAAGA+GGG + chr2.4:32699697-32699716 None:intergenic 30.0%
!!! ATTTTTTCACATTCAGGGTA+AGG + chr2.4:32700632-32700651 None:intergenic 30.0%
!!! GTTGTTTCTTTTTCTGACTA+TGG + chr2.4:32699463-32699482 None:intergenic 30.0%
!!! TGGTGTCGTTAATTTTCTTA+TGG - chr2.4:32700001-32700020 MS.gene99765:CDS 30.0%
AAAAGAGGGTTGTTAAGGAT+TGG + chr2.4:32699683-32699702 None:intergenic 35.0%
AAACCACTTCCTCTTCAATT+CGG - chr2.4:32699641-32699660 MS.gene99765:CDS 35.0%
AAATTCACACCCATGTATTC+AGG + chr2.4:32700479-32700498 None:intergenic 35.0%
ACTTTACGGGAAATTTCAAG+TGG + chr2.4:32699885-32699904 None:intergenic 35.0%
ATACAAAATCCCAAAACCCT+AGG + chr2.4:32700074-32700093 None:intergenic 35.0%
ATTTCCCATTGCAGTTGTAA+TGG - chr2.4:32699962-32699981 MS.gene99765:CDS 35.0%
CAACACCATATTTCATCCTT+TGG + chr2.4:32700439-32700458 None:intergenic 35.0%
CATGAAGTTCCTGAATACAT+GGG - chr2.4:32700467-32700486 MS.gene99765:CDS 35.0%
CATGTTCTTCACATCTTTAC+TGG + chr2.4:32700744-32700763 None:intergenic 35.0%
CATTGTATCTAGGAACAATC+TGG + chr2.4:32700508-32700527 None:intergenic 35.0%
GGTTGTTAGATTGTTTCCTA+GGG - chr2.4:32700055-32700074 MS.gene99765:CDS 35.0%
TCCTAAGGATGAGATTGATA+GGG - chr2.4:32700034-32700053 MS.gene99765:CDS 35.0%
TGTAGAACAACTCAACAAGT+TGG + chr2.4:32699847-32699866 None:intergenic 35.0%
TTCCTAAGGATGAGATTGAT+AGG - chr2.4:32700033-32700052 MS.gene99765:CDS 35.0%
TTTCCCATTGCAGTTGTAAT+GGG - chr2.4:32699963-32699982 MS.gene99765:CDS 35.0%
TTTCTGTAATGAGATGTAGG+TGG + chr2.4:32699549-32699568 None:intergenic 35.0%
! AGATTGTTTCCTAGGGTTTT+GGG - chr2.4:32700062-32700081 MS.gene99765:CDS 35.0%
! ATTGAAGAGGAAGTGGTTTT+TGG + chr2.4:32699640-32699659 None:intergenic 35.0%
! CTTTACGGGAAATTTCAAGT+GGG + chr2.4:32699884-32699903 None:intergenic 35.0%
! GTTTCTTTTTCTGACTATGG+TGG + chr2.4:32699460-32699479 None:intergenic 35.0%
! TAGATTGTTTCCTAGGGTTT+TGG - chr2.4:32700061-32700080 MS.gene99765:CDS 35.0%
! TGAAAGGAAAAGGTGCAATT+GGG - chr2.4:32700540-32700559 MS.gene99765:CDS 35.0%
! TGGGATTTTGTATTGAGGAT+AGG - chr2.4:32700081-32700100 MS.gene99765:CDS 35.0%
! TTGAAAGGAAAAGGTGCAAT+TGG - chr2.4:32700539-32700558 MS.gene99765:CDS 35.0%
!! AAAGAGAAGATTTTTGGGGA+GGG - chr2.4:32700260-32700279 MS.gene99765:CDS 35.0%
!! AGATGTAGGTGGATTAGAAA+TGG + chr2.4:32699538-32699557 None:intergenic 35.0%
!! GTGGAATTTTGAAGGAAAAG+AGG + chr2.4:32699698-32699717 None:intergenic 35.0%
!! TAAAGAGAAGATTTTTGGGG+AGG - chr2.4:32700259-32700278 MS.gene99765:CDS 35.0%
!! TCACATCTTTACTGGTTTGA+GGG + chr2.4:32700736-32700755 None:intergenic 35.0%
!! TGTTTTGTTAAGGGTTTTGG+AGG - chr2.4:32699938-32699957 MS.gene99765:CDS 35.0%
!! TTCACATCTTTACTGGTTTG+AGG + chr2.4:32700737-32700756 None:intergenic 35.0%
!!! GGTTTTGGGATTTTGTATTG+AGG - chr2.4:32700076-32700095 MS.gene99765:CDS 35.0%
AAACCGTAAGCAATCATCCT+TGG + chr2.4:32700383-32700402 None:intergenic 40.0%
AAGGATGATTGCTTACGGTT+TGG - chr2.4:32700382-32700401 MS.gene99765:CDS 40.0%
AATTGAGGTTCCAACACAGA+AGG + chr2.4:32699753-32699772 None:intergenic 40.0%
ACCCTATCAATCTCATCCTT+AGG + chr2.4:32700038-32700057 None:intergenic 40.0%
ATCTCCATTCCCAAAATCCT+TGG + chr2.4:32700182-32700201 None:intergenic 40.0%
ATGGGGGAAACTCAAATTGT+TGG - chr2.4:32699981-32700000 MS.gene99765:CDS 40.0%
ATTGAGGTTCCAACACAGAA+GGG + chr2.4:32699752-32699771 None:intergenic 40.0%
GAACCAAGGATGATTGCTTA+CGG - chr2.4:32700377-32700396 MS.gene99765:CDS 40.0%
GAAGAACATGAAGGCTTTCA+TGG - chr2.4:32700754-32700773 MS.gene99765:CDS 40.0%
GAAGGAAAAGAGGGTTGTTA+AGG + chr2.4:32699688-32699707 None:intergenic 40.0%
GCATGAAGTTCCTGAATACA+TGG - chr2.4:32700466-32700485 MS.gene99765:CDS 40.0%
GCTAATCTCTTCCAAACCTT+CGG - chr2.4:32699582-32699601 MS.gene99765:CDS 40.0%
GGGTTGTTAGATTGTTTCCT+AGG - chr2.4:32700054-32700073 MS.gene99765:CDS 40.0%
TTCCCATTGCAGTTGTAATG+GGG - chr2.4:32699964-32699983 MS.gene99765:CDS 40.0%
! AACAAGTTGGCGATCATCAA+CGG + chr2.4:32699834-32699853 None:intergenic 40.0%
! GTTGCAGAGTTTGAAATGCA+GGG - chr2.4:32700229-32700248 MS.gene99765:CDS 40.0%
! TATGGAATTTGGGGTTCCTA+AGG - chr2.4:32700019-32700038 MS.gene99765:CDS 40.0%
! TGGAGATTGGGGAATTTTCA+CGG - chr2.4:32700195-32700214 MS.gene99765:CDS 40.0%
! TGTTGCAGAGTTTGAAATGC+AGG - chr2.4:32700228-32700247 MS.gene99765:CDS 40.0%
!! AGGATTTTGGGAATGGAGAT+TGG - chr2.4:32700182-32700201 MS.gene99765:CDS 40.0%
!! AGTGGTTTTTGGAGAGGAAA+TGG + chr2.4:32699629-32699648 None:intergenic 40.0%
!! GATTTTGGGAATGGAGATTG+GGG - chr2.4:32700184-32700203 MS.gene99765:CDS 40.0%
!! GGATTTTGGGAATGGAGATT+GGG - chr2.4:32700183-32700202 MS.gene99765:CDS 40.0%
!! GTGGTTTTTGGAGAGGAAAT+GGG + chr2.4:32699628-32699647 None:intergenic 40.0%
!! GTTAAAAGAAAGCATCCAGC+TGG - chr2.4:32700680-32700699 MS.gene99765:CDS 40.0%
!! TGGTTTTTGGAGAGGAAATG+GGG + chr2.4:32699627-32699646 None:intergenic 40.0%
!! TGTAGGTGGATTAGAAATGG+TGG + chr2.4:32699535-32699554 None:intergenic 40.0%
!! TTACTGGTTTGAGGGAACTT+TGG + chr2.4:32700728-32700747 None:intergenic 40.0%
!!! TTTTGAAGTGAAGCTGAGAG+TGG - chr2.4:32700298-32700317 MS.gene99765:CDS 40.0%
AAATGGGGAAGAGAAGAGGA+AGG + chr2.4:32699612-32699631 None:intergenic 45.0%
AACATCTTCCAAAGTCCAGC+TGG + chr2.4:32700698-32700717 None:intergenic 45.0%
AATGGGGAAGAGAAGAGGAA+GGG + chr2.4:32699611-32699630 None:intergenic 45.0%
CCCCTCAACTCATGAATCAA+CGG + chr2.4:32700113-32700132 None:intergenic 45.0%
GATCCGAATTGAAGAGGAAG+TGG + chr2.4:32699647-32699666 None:intergenic 45.0%
GATTCGTAGGGATGCTTTGA+AGG - chr2.4:32700343-32700362 MS.gene99765:CDS 45.0%
TCAAGATTGCCCTTCTGTGT+TGG - chr2.4:32699740-32699759 MS.gene99765:CDS 45.0%
TCCCATTGCAGTTGTAATGG+GGG - chr2.4:32699965-32699984 MS.gene99765:CDS 45.0%
TCCCCCATTACAACTGCAAT+GGG + chr2.4:32699969-32699988 None:intergenic 45.0%
TTCCCCCATTACAACTGCAA+TGG + chr2.4:32699970-32699989 None:intergenic 45.0%
! ACAAGTTGGCGATCATCAAC+GGG + chr2.4:32699833-32699852 None:intergenic 45.0%
! ATTCATGAGTTGAGGGGGTT+GGG - chr2.4:32700116-32700135 MS.gene99765:CDS 45.0%
! GATGATTGCTTACGGTTTGG+AGG - chr2.4:32700385-32700404 MS.gene99765:CDS 45.0%
! GGTGCAATTGGGTTTGAAGT+TGG - chr2.4:32700551-32700570 MS.gene99765:CDS 45.0%
! GTCAGGGATCCAAGGATTTT+GGG - chr2.4:32700170-32700189 MS.gene99765:CDS 45.0%
! TGTCAGGGATCCAAGGATTT+TGG - chr2.4:32700169-32700188 MS.gene99765:CDS 45.0%
! TGTTAAGGATTGGCGAAGGT+GGG + chr2.4:32699673-32699692 None:intergenic 45.0%
! TTGTTAAGGATTGGCGAAGG+TGG + chr2.4:32699674-32699693 None:intergenic 45.0%
! TTTGAGGGAACTTTGGTGGT+TGG + chr2.4:32700721-32700740 None:intergenic 45.0%
!! AGAGGAAGTGGTTTTTGGAG+AGG + chr2.4:32699635-32699654 None:intergenic 45.0%
!! AGCCGTTGATTCATGAGTTG+AGG - chr2.4:32700108-32700127 MS.gene99765:CDS 45.0%
!! CCGTTGATTCATGAGTTGAG+GGG - chr2.4:32700110-32700129 MS.gene99765:CDS 45.0%
!! CGTTGATTCATGAGTTGAGG+GGG - chr2.4:32700111-32700130 MS.gene99765:CDS 45.0%
!! CTGAGAGTGGATTGTTTGTG+CGG - chr2.4:32700311-32700330 MS.gene99765:CDS 45.0%
!! GAAGATTTTTGGGGAGGGTT+TGG - chr2.4:32700265-32700284 MS.gene99765:CDS 45.0%
!! GCCGTTGATTCATGAGTTGA+GGG - chr2.4:32700109-32700128 MS.gene99765:CDS 45.0%
!! GGATCCAAGGATTTTGGGAA+TGG - chr2.4:32700175-32700194 MS.gene99765:CDS 45.0%
!! TGCTTTGAAGGTGTTGTGGA+AGG - chr2.4:32700355-32700374 MS.gene99765:CDS 45.0%
!! TTTTGGGGAGGGTTTGGTTA+GGG - chr2.4:32700271-32700290 MS.gene99765:CDS 45.0%
!! TTTTTGGGGAGGGTTTGGTT+AGG - chr2.4:32700270-32700289 MS.gene99765:CDS 45.0%
AGAAGAGGAAGGGAAACCGA+AGG + chr2.4:32699601-32699620 None:intergenic 50.0%
AGGAAGGGAAACCGAAGGTT+TGG + chr2.4:32699596-32699615 None:intergenic 50.0%
GAAGGCTTTCATGGACTCGT+TGG - chr2.4:32700763-32700782 MS.gene99765:CDS 50.0%
GAGGAAATGGGGAAGAGAAG+AGG + chr2.4:32699616-32699635 None:intergenic 50.0%
GGGATGGATCCGAATTGAAG+AGG + chr2.4:32699653-32699672 None:intergenic 50.0%
! AGGTGTTGTGGAAGGAACCA+AGG - chr2.4:32700363-32700382 MS.gene99765:CDS 50.0%
! CAAGTTGGCGATCATCAACG+GGG + chr2.4:32699832-32699851 None:intergenic 50.0%
! CGGTCACGGTTTGATTCGTA+GGG - chr2.4:32700331-32700350 MS.gene99765:CDS 50.0%
! CTGGTTTGAGGGAACTTTGG+TGG + chr2.4:32700725-32700744 None:intergenic 50.0%
! GATTCATGAGTTGAGGGGGT+TGG - chr2.4:32700115-32700134 MS.gene99765:CDS 50.0%
! GCAGAGTTTGAAATGCAGGG+AGG - chr2.4:32700232-32700251 MS.gene99765:CDS 50.0%
! GGGTTGTTAAGGATTGGCGA+AGG + chr2.4:32699677-32699696 None:intergenic 50.0%
! GTGGATTGTTTGTGCGGTCA+CGG - chr2.4:32700317-32700336 MS.gene99765:CDS 50.0%
! GTTTTCCAGTCGAGAGGTCA+GGG - chr2.4:32700139-32700158 MS.gene99765:CDS 50.0%
! TTCATGAGTTGAGGGGGTTG+GGG - chr2.4:32700117-32700136 MS.gene99765:CDS 50.0%
!! CGGGGAAGCATTAGAAAGCT+TGG + chr2.4:32699814-32699833 None:intergenic 50.0%
!! GAAAGCATCCAGCTGGACTT+TGG - chr2.4:32700687-32700706 MS.gene99765:CDS 50.0%
!! GGGATGCTTTGAAGGTGTTG+TGG - chr2.4:32700351-32700370 MS.gene99765:CDS 50.0%
! AAGGATTGGCGAAGGTGGGA+TGG + chr2.4:32699669-32699688 None:intergenic 55.0%
! GCGGTCACGGTTTGATTCGT+AGG - chr2.4:32700330-32700349 MS.gene99765:CDS 55.0%
! GGTTTTCCAGTCGAGAGGTC+AGG - chr2.4:32700138-32700157 MS.gene99765:CDS 55.0%
! GTTGGGGTTTTCCAGTCGAG+AGG - chr2.4:32700133-32700152 MS.gene99765:CDS 55.0%
CGGAGGTTGTCAGGGATCCA+AGG - chr2.4:32700162-32700181 MS.gene99765:CDS 60.0%
TTCCAGTCGAGAGGTCAGGG+CGG - chr2.4:32700142-32700161 MS.gene99765:CDS 60.0%
AGGTCAGGGCGGAGGTTGTC+AGG - chr2.4:32700153-32700172 MS.gene99765:CDS 65.0%
GGTCAGGGCGGAGGTTGTCA+GGG - chr2.4:32700154-32700173 MS.gene99765:CDS 65.0%
CAGTCGAGAGGTCAGGGCGG+AGG - chr2.4:32700145-32700164 MS.gene99765:CDS 70.0%
CTCCGCCCTGACCTCTCGAC+TGG + chr2.4:32700147-32700166 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 32699444 32700790 32699444 ID=MS.gene99765
chr2.4 mRNA 32699444 32700790 32699444 ID=MS.gene99765.t1;Parent=MS.gene99765
chr2.4 exon 32699444 32700790 32699444 ID=MS.gene99765.t1.exon1;Parent=MS.gene99765.t1
chr2.4 CDS 32699444 32700790 32699444 ID=cds.MS.gene99765.t1;Parent=MS.gene99765.t1
Gene Sequence

>MS.gene99765

ATGGGGTTGGGTTCCACCATAGTCAGAAAAAGAAACAACGTCCTTATTCTTGTAAGAACCACTTCTTCAACTAATAACAACTACTTCTCCACCATTTCTAATCCACCTACATCTCATTACAGAAAACAAATCGCACTCGCTAATCTCTTCCAAACCTTCGGTTTCCCTTCCTCTTCTCTTCCCCATTTCCTCTCCAAAAACCACTTCCTCTTCAATTCGGATCCATCCCACCTTCGCCAATCCTTAACAACCCTCTTTTCCTTCAAAATTCCACAGAAAAACCTAATTTCACTTCTTCAAGATTGCCCTTCTGTGTTGGAACCTCAATTTCTACTTAATTGGGAATTGGGTTTTCCAAAATTCATATCCAAGCTTTCTAATGCTTCCCCGTTGATGATCGCCAACTTGTTGAGTTGTTCTACAACTTTCCATTTAAACCCACTTGAAATTTCCCGTAAAGTTGAAATTTTTAGGGGTTTAGGATTTTCTGATGATGTTTTGTTAAGGGTTTTGGAGGAATTTCCCATTGCAGTTGTAATGGGGGAAACTCAAATTGTTGGTGTCGTTAATTTTCTTATGGAATTTGGGGTTCCTAAGGATGAGATTGATAGGGTTGTTAGATTGTTTCCTAGGGTTTTGGGATTTTGTATTGAGGATAGGTTGAAGCCGTTGATTCATGAGTTGAGGGGGTTGGGGTTTTCCAGTCGAGAGGTCAGGGCGGAGGTTGTCAGGGATCCAAGGATTTTGGGAATGGAGATTGGGGAATTTTCACGGTGTTTGAAGTTGTTGCAGAGTTTGAAATGCAGGGAGGCAATTAAAGAGAAGATTTTTGGGGAGGGTTTGGTTAGGGCATGTTTTGAAGTGAAGCTGAGAGTGGATTGTTTGTGCGGTCACGGTTTGATTCGTAGGGATGCTTTGAAGGTGTTGTGGAAGGAACCAAGGATGATTGCTTACGGTTTGGAGGATATTGAGAAGAAGATTGAGTTTTTAATCCAAAGGATGAAATATGGTGTTGATTGTTTGCATGAAGTTCCTGAATACATGGGTGTGAATTTTGAAAACCAGATTGTTCCTAGATACAATGTGATTGAGTATTTGAAAGGAAAAGGTGCAATTGGGTTTGAAGTTGGATTGAAAGACATTATCAAACCAACTAGACTTAGATTTTATAATCTTTATGTCAAGCCTTACCCTGAATGTGAAAAAATATATGGCAGATTTTCAGAAAAGGTTGAAGTTAAAAGAAAGCATCCAGCTGGACTTTGGAAGATGTTCCAACCACCAAAGTTCCCTCAAACCAGTAAAGATGTGAAGAACATGAAGGCTTTCATGGACTCGTTGGTGTAG
Protein sequence

>MS.gene99765.t1

MGLGSTIVRKRNNVLILVRTTSSTNNNYFSTISNPPTSHYRKQIALANLFQTFGFPSSSLPHFLSKNHFLFNSDPSHLRQSLTTLFSFKIPQKNLISLLQDCPSVLEPQFLLNWELGFPKFISKLSNASPLMIANLLSCSTTFHLNPLEISRKVEIFRGLGFSDDVLLRVLEEFPIAVVMGETQIVGVVNFLMEFGVPKDEIDRVVRLFPRVLGFCIEDRLKPLIHELRGLGFSSREVRAEVVRDPRILGMEIGEFSRCLKLLQSLKCREAIKEKIFGEGLVRACFEVKLRVDCLCGHGLIRRDALKVLWKEPRMIAYGLEDIEKKIEFLIQRMKYGVDCLHEVPEYMGVNFENQIVPRYNVIEYLKGKGAIGFEVGLKDIIKPTRLRFYNLYVKPYPECEKIYGRFSEKVEVKRKHPAGLWKMFQPPKFPQTSKDVKNMKAFMDSLV