AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99828

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99828.t1 MTR_7g028740 96.467 368 10 3 1 366 1 367 0.0 729
MS.gene99828.t1 MTR_3g099080 35.427 398 203 12 1 366 1 376 2.99e-59 196
MS.gene99828.t1 MTR_8g096780 48.756 201 95 6 17 211 36 234 2.73e-50 173
MS.gene99828.t1 MTR_4g109770 40.191 209 105 6 25 223 75 273 3.49e-37 140
MS.gene99828.t1 MTR_2g461920 52.308 130 60 1 70 199 166 293 3.79e-35 135
MS.gene99828.t1 MTR_3g085180 59.341 91 34 1 67 154 154 244 6.38e-33 127
MS.gene99828.t1 MTR_5g046670 57.000 100 43 0 74 173 243 342 8.42e-33 128
MS.gene99828.t1 MTR_7g092930 63.636 88 32 0 70 157 63 150 8.57e-33 125
MS.gene99828.t1 MTR_4g088555 59.551 89 34 1 62 148 48 136 4.53e-32 118
MS.gene99828.t1 MTR_8g463140 63.750 80 29 0 74 153 64 143 6.68e-32 119
MS.gene99828.t1 MTR_2g014200 64.198 81 29 0 69 149 58 138 5.27e-31 115
MS.gene99828.t1 MTR_1g053715 62.651 83 31 0 75 157 74 156 7.05e-31 120
MS.gene99828.t1 MTR_1g035010 43.046 151 70 4 2 151 45 180 1.59e-30 123
MS.gene99828.t1 MTR_2g046550 45.926 135 58 2 17 151 96 215 2.69e-30 122
MS.gene99828.t1 MTR_7g444860 63.750 80 29 0 75 154 149 228 3.31e-30 117
MS.gene99828.t1 MTR_2g078770 66.667 75 25 0 74 148 57 131 1.12e-29 112
MS.gene99828.t1 MTR_8g005960 56.436 101 39 2 58 153 165 265 3.03e-29 115
MS.gene99828.t1 MTR_1g086250 60.440 91 35 1 59 148 130 220 1.13e-28 118
MS.gene99828.t1 MTR_7g110320 59.551 89 34 1 62 148 130 218 2.39e-28 117
MS.gene99828.t1 MTR_7g110320 59.551 89 34 1 62 148 130 218 2.94e-28 116
MS.gene99828.t1 MTR_8g080690 62.338 77 29 0 75 151 151 227 4.23e-28 113
MS.gene99828.t1 MTR_8g080680 56.757 74 32 0 75 148 132 205 4.95e-24 102
MS.gene99828.t1 MTR_8g080670 50.575 87 39 1 65 147 148 234 8.01e-24 102
MS.gene99828.t1 MTR_2g020620 49.231 65 33 0 84 148 99 163 2.37e-16 80.9
MS.gene99828.t1 MTR_8g066240 44.211 95 49 2 25 115 8 102 1.78e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99828.t1 AT5G50670 46.354 192 87 5 24 200 41 231 2.17e-43 154
MS.gene99828.t1 AT5G50670 46.354 192 87 5 24 200 41 231 2.17e-43 154
MS.gene99828.t1 AT5G50570 46.354 192 87 5 24 200 41 231 2.17e-43 154
MS.gene99828.t1 AT5G50570 46.354 192 87 5 24 200 41 231 2.17e-43 154
MS.gene99828.t1 AT1G69170 67.416 89 28 1 62 149 110 198 5.01e-34 130
MS.gene99828.t1 AT1G76580 44.156 154 85 1 2 154 40 193 1.70e-32 129
MS.gene99828.t1 AT1G76580 44.156 154 85 1 2 154 40 193 1.75e-32 129
MS.gene99828.t1 AT1G76580 44.156 154 85 1 2 154 40 193 2.24e-32 129
MS.gene99828.t1 AT2G42200 63.218 87 32 0 75 161 74 160 3.99e-32 124
MS.gene99828.t1 AT5G43270 47.788 113 59 0 75 187 169 281 5.01e-32 125
MS.gene99828.t1 AT5G43270 47.788 113 59 0 75 187 169 281 5.01e-32 125
MS.gene99828.t1 AT5G43270 47.788 113 59 0 75 187 169 281 5.01e-32 125
MS.gene99828.t1 AT1G20980 44.375 160 69 3 2 152 49 197 7.80e-32 127
MS.gene99828.t1 AT3G60030 59.375 96 37 1 61 154 111 206 2.04e-31 126
MS.gene99828.t1 AT1G27370 56.075 107 43 2 48 150 145 251 4.31e-31 121
MS.gene99828.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.53e-31 121
MS.gene99828.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.53e-31 121
MS.gene99828.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.53e-31 121
MS.gene99828.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.53e-31 121
MS.gene99828.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.53e-31 121
MS.gene99828.t1 AT1G27370 56.075 107 43 2 48 150 145 251 6.53e-31 121
MS.gene99828.t1 AT2G33810 62.667 75 28 0 74 148 53 127 6.94e-31 114
MS.gene99828.t1 AT1G27360 67.105 76 25 0 75 150 175 250 7.14e-30 118
MS.gene99828.t1 AT1G27360 67.105 76 25 0 75 150 175 250 7.14e-30 118
MS.gene99828.t1 AT1G27360 67.105 76 25 0 75 150 175 250 7.14e-30 118
MS.gene99828.t1 AT1G27360 67.105 76 25 0 75 150 175 250 7.14e-30 118
MS.gene99828.t1 AT1G53160 52.688 93 44 0 63 155 42 134 2.58e-29 111
MS.gene99828.t1 AT1G53160 52.688 93 44 0 63 155 42 134 4.70e-29 111
MS.gene99828.t1 AT1G53160 52.688 93 44 0 63 155 42 134 4.70e-29 111
MS.gene99828.t1 AT2G47070 65.385 78 27 0 74 151 105 182 5.67e-29 119
MS.gene99828.t1 AT3G15270 61.333 75 29 0 74 148 62 136 2.14e-28 109
MS.gene99828.t1 AT3G57920 51.961 102 44 2 75 176 59 155 2.23e-28 114
MS.gene99828.t1 AT1G02065 64.865 74 26 0 75 148 188 261 8.31e-28 112
MS.gene99828.t1 AT5G18830 48.315 89 45 1 62 149 125 213 5.16e-21 95.1
MS.gene99828.t1 AT5G18830 48.315 89 45 1 62 149 125 213 6.48e-21 94.7
MS.gene99828.t1 AT5G18830 48.315 89 45 1 62 149 125 213 6.49e-21 94.7
MS.gene99828.t1 AT2G47070 73.077 52 14 0 100 151 2 53 5.92e-17 82.8
MS.gene99828.t1 AT1G02065 45.570 79 36 2 58 129 166 244 3.64e-15 74.7

Find 90 sgRNAs with CRISPR-Local

Find 153 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCTTATCTTTCGCCTTTC+CGG 0.146373 7.2:+71401613 None:intergenic
CAGTGACTCTTCCATTCTTC+TGG 0.209211 7.2:-71401493 MS.gene99828:CDS
GAGAAATCAGTTCATGATTC+AGG 0.275617 7.2:-71403913 MS.gene99828:CDS
AACCAACACAACAGGAGTTT+TGG 0.287936 7.2:+71403723 None:intergenic
CTACCAAAGTGCAGGATTCT+AGG 0.315434 7.2:+71402064 None:intergenic
GGCCAAATGTTTCTCATAAT+AGG 0.321028 7.2:+71402022 None:intergenic
CTCTCTTCTGATGGCAAGCC+CGG 0.326414 7.2:-71401788 MS.gene99828:CDS
GGATTTGAGGCTTGGTGATC+AGG 0.330157 7.2:-71403950 MS.gene99828:CDS
ATGAGGTTAAGAGGAGTTGC+AGG 0.333125 7.2:-71403256 MS.gene99828:CDS
GGTTCATCCGCAGGTGAAAA+AGG 0.348606 7.2:-71401821 MS.gene99828:CDS
AAAAGAGCGCGTTTGCAAAA+TGG 0.355601 7.2:-71403841 MS.gene99828:CDS
TCAGCCACCTTCTCTCTTCA+TGG 0.360485 7.2:-71403195 MS.gene99828:CDS
GGACCGGTATTTGTTCTAGA+TGG 0.361540 7.2:-71401587 MS.gene99828:CDS
TCATATACCTGCTGCATTGT+TGG 0.364223 7.2:+71403677 None:intergenic
GAGAGTATCATAAGCGTCAT+AGG 0.369267 7.2:-71403762 MS.gene99828:CDS
GCTTCAGTTATGGAAGTTGC+AGG 0.374630 7.2:+71402001 None:intergenic
CGACAAACATAAACAGGATA+AGG 0.380487 7.2:-71401939 MS.gene99828:CDS
TGATCAGGCTTCTGATTCAT+TGG 0.384148 7.2:-71403935 MS.gene99828:CDS
TCCATAACTGAAGCTGAATC+TGG 0.398324 7.2:-71401992 MS.gene99828:CDS
GCAGCATCCCATTGCAATGA+AGG 0.401478 7.2:+71401552 None:intergenic
TTGTTAACATCGCCGGACTT+AGG 0.408591 7.2:+71401890 None:intergenic
ACCAGATTCAGCTTCAGTTA+TGG 0.414958 7.2:+71401991 None:intergenic
TCGAATCGACAAACATAAAC+AGG 0.415548 7.2:-71401945 MS.gene99828:CDS
TGTTAACATCGCCGGACTTA+GGG 0.417871 7.2:+71401891 None:intergenic
AGTGACTCTTCCATTCTTCT+GGG 0.418501 7.2:-71401492 MS.gene99828:CDS
GGGATTGGAAAGAGTTTGCT+TGG 0.421291 7.2:-71404017 MS.gene99828:CDS
CGGAGTTTGATGAGGTTAAG+AGG 0.427340 7.2:-71403265 MS.gene99828:CDS
TGCTTGGGATCCATCTGGAT+TGG 0.431081 7.2:-71404001 MS.gene99828:CDS
AATATGATTTGCTCGGTTGA+TGG 0.434096 7.2:-71403811 MS.gene99828:CDS
AAAACTCCTGTTGTGTTGGT+TGG 0.434108 7.2:-71403721 MS.gene99828:CDS
GAGTTTGCTTGGGATCCATC+TGG 0.438111 7.2:-71404006 MS.gene99828:CDS
ATCCTATTATGAGAAACATT+TGG 0.440090 7.2:-71402024 MS.gene99828:CDS
GATTCAATGGATTTGAGGCT+TGG 0.443840 7.2:-71403958 MS.gene99828:CDS
AAGGAGATTCAATGGATTTG+AGG 0.446707 7.2:-71403963 MS.gene99828:CDS
ATGCTGCAAATGGGTCCTAA+TGG 0.447508 7.2:-71401536 MS.gene99828:CDS
CAACAAACCTTCATTGCAAT+GGG 0.452275 7.2:-71401559 MS.gene99828:CDS
TTGGGATCCATCTGGATTGG+AGG 0.460222 7.2:-71403998 MS.gene99828:CDS
TTGCAATGGGATGCTGCAAA+TGG 0.467365 7.2:-71401546 MS.gene99828:CDS
GATGTTAACAAAGCAGCATC+TGG 0.469387 7.2:-71401878 MS.gene99828:CDS
ACAACCCGAGTCAAAAGATC+CGG 0.476548 7.2:+71401769 None:intergenic
GACAAACATAAACAGGATAA+GGG 0.477832 7.2:-71401938 MS.gene99828:CDS
TGCTGCTTTGTTAACATCGC+CGG 0.479167 7.2:+71401883 None:intergenic
ACGAAGGAGTACTCGTACTC+CGG 0.480410 7.2:-71401632 MS.gene99828:CDS
CATCCTATCCGCGGTGCCGT+TGG 0.484865 7.2:-71401842 MS.gene99828:CDS
AGTTGCAGGAAACGTCTTGA+TGG 0.488371 7.2:-71403242 MS.gene99828:CDS
AGAACAAATACCGGTCCATT+CGG 0.494466 7.2:+71401593 None:intergenic
AGGATAAGGGACATCCTCAT+TGG 0.499189 7.2:-71401925 MS.gene99828:CDS
CCGCGGATAGGATGGCTGAT+AGG 0.503089 7.2:+71401851 None:intergenic
AGACGCTACTCCCAGAAGAA+TGG 0.507419 7.2:+71401482 None:intergenic
GGATTGGAAAGAGTTTGCTT+GGG 0.508642 7.2:-71404016 MS.gene99828:CDS
TTACCTCCAACCAACACAAC+AGG 0.509843 7.2:+71403715 None:intergenic
GAAAGATAAGCCGAATGGAC+CGG 0.517456 7.2:-71401603 MS.gene99828:CDS
CAACGGCACCGCGGATAGGA+TGG 0.519767 7.2:+71401843 None:intergenic
ACAACAAACCTTCATTGCAA+TGG 0.522232 7.2:-71401560 MS.gene99828:CDS
ATGAAGAGAGAAGGTGGCTG+AGG 0.523686 7.2:+71403197 None:intergenic
AGTCCAACACAATCTTCGTC+TGG 0.529463 7.2:-71401674 MS.gene99828:CDS
ACTCCTGTTGTGTTGGTTGG+AGG 0.530507 7.2:-71403718 MS.gene99828:CDS
CAAGAAATTCTCCCTAAGTC+CGG 0.531212 7.2:-71401902 MS.gene99828:CDS
TTCACCTGCGGATGAACCAA+CGG 0.533214 7.2:+71401826 None:intergenic
TTCAACTCCTCCAATCCAGA+TGG 0.535266 7.2:+71403991 None:intergenic
GGGAGAATTTCTTGCCAATG+AGG 0.536926 7.2:+71401911 None:intergenic
GAACCAACGGCACCGCGGAT+AGG 0.537355 7.2:+71401839 None:intergenic
GGTGCCGTTGGTTCATCCGC+AGG 0.539865 7.2:-71401830 MS.gene99828:CDS
GGACCTAGAATCCTGCACTT+TGG 0.540161 7.2:-71402067 MS.gene99828:CDS
TTCCAAAACTCCTGTTGTGT+TGG 0.540226 7.2:-71403725 MS.gene99828:CDS
TGCAATGGGATGCTGCAAAT+GGG 0.554526 7.2:-71401545 MS.gene99828:CDS
GGTTCTGCCAACAATGCAGC+AGG 0.557731 7.2:-71403684 MS.gene99828:intron
ATGTAGGTTTCATTCACTTG+CGG 0.558210 7.2:-71403285 MS.gene99828:intron
TCTGAGGGCTACCAAAGTGC+AGG 0.563556 7.2:+71402056 None:intergenic
GGAGTACTCGTACTCCGGAA+AGG 0.575335 7.2:-71401627 MS.gene99828:CDS
ATCGCAGAATATGATTTGCT+CGG 0.580677 7.2:-71403818 MS.gene99828:CDS
GGTAGGAAGCTCTCTTCTGA+TGG 0.581427 7.2:-71401797 MS.gene99828:CDS
TTTCCATCTAGAACAAATAC+CGG 0.581750 7.2:+71401584 None:intergenic
ATCAGTTCATGATTCAGGAA+AGG 0.585059 7.2:-71403908 MS.gene99828:CDS
AGAGTATCATAAGCGTCATA+GGG 0.590533 7.2:-71403761 MS.gene99828:CDS
TTCACTTGCGGAGTTTGATG+AGG 0.591231 7.2:-71403273 MS.gene99828:CDS
TGGTTGGAGGTAAGCAGCAG+AGG 0.595809 7.2:-71403705 MS.gene99828:CDS
TCAGCAGCCATGAAGAGAGA+AGG 0.603814 7.2:+71403188 None:intergenic
CAACCCGAGTCAAAAGATCC+GGG 0.610877 7.2:+71401770 None:intergenic
GCAGCCATGAAGAGAGAAGG+TGG 0.612633 7.2:+71403191 None:intergenic
TAGGATTGACATATGTCTGA+GGG 0.617179 7.2:+71402041 None:intergenic
AAGTTCATGTACAATTACAA+AGG 0.619439 7.2:-71403164 MS.gene99828:intron
ATAGGATTGACATATGTCTG+AGG 0.620773 7.2:+71402040 None:intergenic
TTGATGGACATAATAAGCGC+AGG 0.628418 7.2:-71403226 MS.gene99828:CDS
TTCGTAGCATCAAAATGCAA+CGG 0.628644 7.2:+71401650 None:intergenic
AAGGCGAAAGATAAGCCGAA+TGG 0.638149 7.2:-71401608 MS.gene99828:CDS
GGTCCAGACGAAGATTGTGT+TGG 0.652140 7.2:+71401671 None:intergenic
CCTATCAGCCATCCTATCCG+CGG 0.675251 7.2:-71401851 MS.gene99828:CDS
ATGGACATAATAAGCGCAGG+AGG 0.679251 7.2:-71403223 MS.gene99828:CDS
CGGATGAACCAACGGCACCG+CGG 0.749670 7.2:+71401834 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCTTTGTCATTAAATTTGAT+TGG + chr7.2:71402984-71403003 None:intergenic 20.0%
!!! TTTTCTCTTTGTACAATTTT+AGG - chr7.2:71403423-71403442 MS.gene99828:intron 20.0%
! AAGTTCATGTACAATTACAA+AGG - chr7.2:71402347-71402366 MS.gene99828:intron 25.0%
! AATGAGATGAAAACAACTTA+TGG - chr7.2:71402064-71402083 MS.gene99828:CDS 25.0%
! ATATTCAGAGAGCTTATATA+TGG + chr7.2:71402108-71402127 None:intergenic 25.0%
! ATCCTATTATGAGAAACATT+TGG - chr7.2:71403487-71403506 MS.gene99828:intron 25.0%
! ATTCTATTGTCAAAACCTTA+TGG + chr7.2:71402556-71402575 None:intergenic 25.0%
! CATATACATAACAATCCATA+AGG - chr7.2:71402538-71402557 MS.gene99828:intron 25.0%
! CTTTCTTGTGAAAATAAAGA+CGG + chr7.2:71403017-71403036 None:intergenic 25.0%
! TGAATTGAAGTTGTTTGTTT+CGG - chr7.2:71401854-71401873 MS.gene99828:CDS 25.0%
!! CTCTCTGAATATTCTAAAAA+TGG - chr7.2:71402116-71402135 MS.gene99828:intron 25.0%
!! TATAGTTATTTAGCGACTTT+TGG - chr7.2:71402652-71402671 MS.gene99828:intron 25.0%
!! TTTTTGCAATTGAAATCCAT+GGG + chr7.2:71403074-71403093 None:intergenic 25.0%
!!! CGAAGATATAACGTTTTTTA+TGG - chr7.2:71402481-71402500 MS.gene99828:intron 25.0%
!!! CTTTATTTTCACAAGAAAGA+TGG - chr7.2:71403018-71403037 MS.gene99828:intron 25.0%
!!! TTGAGGTTGTTTTGTAATAT+GGG - chr7.2:71402188-71402207 MS.gene99828:intron 25.0%
!!! TTTGAGGTTGTTTTGTAATA+TGG - chr7.2:71402187-71402206 MS.gene99828:intron 25.0%
!!! TTTTTTGCAATTGAAATCCA+TGG + chr7.2:71403075-71403094 None:intergenic 25.0%
AGTAACTGTTGGATTTAGAT+CGG - chr7.2:71403127-71403146 MS.gene99828:intron 30.0%
ATTAGTGCTTTAGTAACTGT+TGG - chr7.2:71403116-71403135 MS.gene99828:intron 30.0%
CATTAAATTTGATTGGAGAG+AGG + chr7.2:71402977-71402996 None:intergenic 30.0%
GAAAAATGAAGGAGATTCAA+TGG - chr7.2:71401540-71401559 MS.gene99828:CDS 30.0%
GAATTGAAGTTGTTTGTTTC+GGG - chr7.2:71401855-71401874 MS.gene99828:CDS 30.0%
GACAAACATAAACAGGATAA+GGG - chr7.2:71403573-71403592 MS.gene99828:intron 30.0%
TACAAAACAACCTCAAAACT+CGG + chr7.2:71402184-71402203 None:intergenic 30.0%
TTAGTTAAGTTTGAACATGC+CGG + chr7.2:71403306-71403325 None:intergenic 30.0%
! TATTTTCACAAGAAAGATGG+TGG - chr7.2:71403021-71403040 MS.gene99828:intron 30.0%
! TCATTTTCGATCAATCCATT+AGG + chr7.2:71403993-71404012 None:intergenic 30.0%
! TTTCCATCTAGAACAAATAC+CGG + chr7.2:71403930-71403949 None:intergenic 30.0%
! TTTTGCAATTGAAATCCATG+GGG + chr7.2:71403073-71403092 None:intergenic 30.0%
!! TCTCATGATTCTAAGATAGA+TGG - chr7.2:71402385-71402404 MS.gene99828:intron 30.0%
!! TGAGGTTGTTTTGTAATATG+GGG - chr7.2:71402189-71402208 MS.gene99828:intron 30.0%
AATTTGATTGGAGAGAGGAT+TGG + chr7.2:71402972-71402991 None:intergenic 35.0%
ACAACAAACCTTCATTGCAA+TGG - chr7.2:71403951-71403970 MS.gene99828:CDS 35.0%
ACTTTGCTAATATTTCCCCA+TGG - chr7.2:71403055-71403074 MS.gene99828:intron 35.0%
AGGGAATGCATAAAAACTCT+GGG - chr7.2:71402755-71402774 MS.gene99828:intron 35.0%
ATAATTCCCTCTTTCATCTC+AGG + chr7.2:71402459-71402478 None:intergenic 35.0%
ATAGGATTGACATATGTCTG+AGG + chr7.2:71403474-71403493 None:intergenic 35.0%
ATCAGTTCATGATTCAGGAA+AGG - chr7.2:71401603-71401622 MS.gene99828:CDS 35.0%
ATGTAGGTTTCATTCACTTG+CGG - chr7.2:71402226-71402245 MS.gene99828:intron 35.0%
CAACAAACCTTCATTGCAAT+GGG - chr7.2:71403952-71403971 MS.gene99828:CDS 35.0%
CGACAAACATAAACAGGATA+AGG - chr7.2:71403572-71403591 MS.gene99828:intron 35.0%
GAATCTTGATCCTTGATATC+CGG - chr7.2:71403284-71403303 MS.gene99828:intron 35.0%
GAGAAATCAGTTCATGATTC+AGG - chr7.2:71401598-71401617 MS.gene99828:CDS 35.0%
GCTTAGTGCAATAAGTTTCT+AGG - chr7.2:71402792-71402811 MS.gene99828:intron 35.0%
GGCCAAATGTTTCTCATAAT+AGG + chr7.2:71403492-71403511 None:intergenic 35.0%
TAAACACTTGCGCAAGTATT+TGG + chr7.2:71402884-71402903 None:intergenic 35.0%
TAGGATTGACATATGTCTGA+GGG + chr7.2:71403473-71403492 None:intergenic 35.0%
TCGAATCGACAAACATAAAC+AGG - chr7.2:71403566-71403585 MS.gene99828:intron 35.0%
TTAGCTCAGAATTCAGTTCA+TGG + chr7.2:71402618-71402637 None:intergenic 35.0%
TTCGTAGCATCAAAATGCAA+CGG + chr7.2:71403864-71403883 None:intergenic 35.0%
TTGGAGGAGTTGAAAAATGA+AGG - chr7.2:71401529-71401548 MS.gene99828:CDS 35.0%
! AAGGAGATTCAATGGATTTG+AGG - chr7.2:71401548-71401567 MS.gene99828:CDS 35.0%
! AGAGTATCATAAGCGTCATA+GGG - chr7.2:71401750-71401769 MS.gene99828:CDS 35.0%
! AGCTGATTCTACTATTGAAG+AGG - chr7.2:71402735-71402754 MS.gene99828:intron 35.0%
! GCTGATTCTACTATTGAAGA+GGG - chr7.2:71402736-71402755 MS.gene99828:intron 35.0%
! GGTGTGGTTCAATTTTATGT+AGG - chr7.2:71402210-71402229 MS.gene99828:intron 35.0%
!! AATATGATTTGCTCGGTTGA+TGG - chr7.2:71401700-71401719 MS.gene99828:CDS 35.0%
!! ATCGCAGAATATGATTTGCT+CGG - chr7.2:71401693-71401712 MS.gene99828:CDS 35.0%
!! TTGTTTTGTAATATGGGGTG+TGG - chr7.2:71402194-71402213 MS.gene99828:intron 35.0%
!!! TTTTTGTTTTCCACAAGCAC+AGG - chr7.2:71403379-71403398 MS.gene99828:intron 35.0%
ACCAGATTCAGCTTCAGTTA+TGG + chr7.2:71403523-71403542 None:intergenic 40.0%
ACCTCAAAACTCGGTATGTT+TGG + chr7.2:71402175-71402194 None:intergenic 40.0%
AGAACAAATACCGGTCCATT+CGG + chr7.2:71403921-71403940 None:intergenic 40.0%
AGCACTAATCGTGTAAATGC+AGG + chr7.2:71403105-71403124 None:intergenic 40.0%
AGTGACTCTTCCATTCTTCT+GGG - chr7.2:71404019-71404038 MS.gene99828:CDS 40.0%
AGTGACTGTTGGATCAAGAT+TGG + chr7.2:71403223-71403242 None:intergenic 40.0%
CAAGAAATTCTCCCTAAGTC+CGG - chr7.2:71403609-71403628 MS.gene99828:intron 40.0%
GAGGGAATGCATAAAAACTC+TGG - chr7.2:71402754-71402773 MS.gene99828:intron 40.0%
GATCAAGATTCTCTGCAGTT+GGG + chr7.2:71403275-71403294 None:intergenic 40.0%
GATGTTAACAAAGCAGCATC+TGG - chr7.2:71403633-71403652 MS.gene99828:intron 40.0%
GCACTAATCGTGTAAATGCA+GGG + chr7.2:71403104-71403123 None:intergenic 40.0%
GTCAAACCTGAGATGAAAGA+GGG - chr7.2:71402450-71402469 MS.gene99828:intron 40.0%
TACTGTGCTGAAAATTCGCA+CGG - chr7.2:71403196-71403215 MS.gene99828:CDS 40.0%
TCATATACCTGCTGCATTGT+TGG + chr7.2:71401837-71401856 None:intergenic 40.0%
TCCATAACTGAAGCTGAATC+TGG - chr7.2:71403519-71403538 MS.gene99828:intron 40.0%
TGATCAGGCTTCTGATTCAT+TGG - chr7.2:71401576-71401595 MS.gene99828:CDS 40.0%
TGTCAAACCTGAGATGAAAG+AGG - chr7.2:71402449-71402468 MS.gene99828:intron 40.0%
TTCCAAAACTCCTGTTGTGT+TGG - chr7.2:71401786-71401805 MS.gene99828:CDS 40.0%
TTCCACAAGCACAGGTAATA+TGG - chr7.2:71403387-71403406 MS.gene99828:intron 40.0%
TTTGAACATGCCGGATATCA+AGG + chr7.2:71403297-71403316 None:intergenic 40.0%
! AAAACTCCTGTTGTGTTGGT+TGG - chr7.2:71401790-71401809 MS.gene99828:CDS 40.0%
! AAAAGAGCGCGTTTGCAAAA+TGG - chr7.2:71401670-71401689 MS.gene99828:CDS 40.0%
! AACCAACACAACAGGAGTTT+TGG + chr7.2:71401791-71401810 None:intergenic 40.0%
! GATTCAATGGATTTGAGGCT+TGG - chr7.2:71401553-71401572 MS.gene99828:CDS 40.0%
! GCTCTTTTCAACGATCTAGT+AGG + chr7.2:71401658-71401677 None:intergenic 40.0%
! GGATTGGAAAGAGTTTGCTT+GGG - chr7.2:71401495-71401514 MS.gene99828:CDS 40.0%
! TCCAAACATACCGAGTTTTG+AGG - chr7.2:71402171-71402190 MS.gene99828:intron 40.0%
! TTGATGGACATAATAAGCGC+AGG - chr7.2:71402285-71402304 MS.gene99828:intron 40.0%
!! GAGAGTATCATAAGCGTCAT+AGG - chr7.2:71401749-71401768 MS.gene99828:CDS 40.0%
!! GTTGCATTTTGATGCTACGA+AGG - chr7.2:71403863-71403882 MS.gene99828:CDS 40.0%
AAGGCGAAAGATAAGCCGAA+TGG - chr7.2:71403903-71403922 MS.gene99828:CDS 45.0%
ACAACCCGAGTCAAAAGATC+CGG + chr7.2:71403745-71403764 None:intergenic 45.0%
AGGATAAGGGACATCCTCAT+TGG - chr7.2:71403586-71403605 MS.gene99828:intron 45.0%
AGTCCAACACAATCTTCGTC+TGG - chr7.2:71403837-71403856 MS.gene99828:CDS 45.0%
AGTTGCAGGAAACGTCTTGA+TGG - chr7.2:71402269-71402288 MS.gene99828:intron 45.0%
ATGAGGTTAAGAGGAGTTGC+AGG - chr7.2:71402255-71402274 MS.gene99828:intron 45.0%
ATGCTGCAAATGGGTCCTAA+TGG - chr7.2:71403975-71403994 MS.gene99828:CDS 45.0%
ATGGACATAATAAGCGCAGG+AGG - chr7.2:71402288-71402307 MS.gene99828:intron 45.0%
CAGTGACTCTTCCATTCTTC+TGG - chr7.2:71404018-71404037 MS.gene99828:CDS 45.0%
CGGAGTTTGATGAGGTTAAG+AGG - chr7.2:71402246-71402265 MS.gene99828:intron 45.0%
CTACCAAAGTGCAGGATTCT+AGG + chr7.2:71403450-71403469 None:intergenic 45.0%
CTCCATATTACCTGTGCTTG+TGG + chr7.2:71403392-71403411 None:intergenic 45.0%
GAAAGATAAGCCGAATGGAC+CGG - chr7.2:71403908-71403927 MS.gene99828:CDS 45.0%
GACAACACATCAGTGACTGT+TGG + chr7.2:71403234-71403253 None:intergenic 45.0%
GCTTCAGTTATGGAAGTTGC+AGG + chr7.2:71403513-71403532 None:intergenic 45.0%
GGATCAAGATTCTCTGCAGT+TGG + chr7.2:71403276-71403295 None:intergenic 45.0%
GGGAGAATTTCTTGCCAATG+AGG + chr7.2:71403603-71403622 None:intergenic 45.0%
TGCAATGGGATGCTGCAAAT+GGG - chr7.2:71403966-71403985 MS.gene99828:CDS 45.0%
TGTTAACATCGCCGGACTTA+GGG + chr7.2:71403623-71403642 None:intergenic 45.0%
TTACCTCCAACCAACACAAC+AGG + chr7.2:71401799-71401818 None:intergenic 45.0%
TTCAACTCCTCCAATCCAGA+TGG + chr7.2:71401523-71401542 None:intergenic 45.0%
TTCACTTGCGGAGTTTGATG+AGG - chr7.2:71402238-71402257 MS.gene99828:intron 45.0%
TTGCAATGGGATGCTGCAAA+TGG - chr7.2:71403965-71403984 MS.gene99828:CDS 45.0%
TTGTTAACATCGCCGGACTT+AGG + chr7.2:71403624-71403643 None:intergenic 45.0%
! GGACCGGTATTTGTTCTAGA+TGG - chr7.2:71403924-71403943 MS.gene99828:CDS 45.0%
! GGGATTGGAAAGAGTTTGCT+TGG - chr7.2:71401494-71401513 MS.gene99828:CDS 45.0%
!! TGCTGCTTTGTTAACATCGC+CGG + chr7.2:71403631-71403650 None:intergenic 45.0%
ATGAAGAGAGAAGGTGGCTG+AGG + chr7.2:71402317-71402336 None:intergenic 50.0%
CAACCCGAGTCAAAAGATCC+GGG + chr7.2:71403744-71403763 None:intergenic 50.0%
CGGCTTATCTTTCGCCTTTC+CGG + chr7.2:71403901-71403920 None:intergenic 50.0%
GCAGCATCCCATTGCAATGA+AGG + chr7.2:71403962-71403981 None:intergenic 50.0%
GGACCTAGAATCCTGCACTT+TGG - chr7.2:71403444-71403463 MS.gene99828:intron 50.0%
GGTAGGAAGCTCTCTTCTGA+TGG - chr7.2:71403714-71403733 MS.gene99828:CDS 50.0%
GGTCCAGACGAAGATTGTGT+TGG + chr7.2:71403843-71403862 None:intergenic 50.0%
GGTTCATCCGCAGGTGAAAA+AGG - chr7.2:71403690-71403709 MS.gene99828:CDS 50.0%
TCAGCAGCCATGAAGAGAGA+AGG + chr7.2:71402326-71402345 None:intergenic 50.0%
TCAGCCACCTTCTCTCTTCA+TGG - chr7.2:71402316-71402335 MS.gene99828:intron 50.0%
TCATCCGCAGGTGAAAAAGG+TGG - chr7.2:71403693-71403712 MS.gene99828:CDS 50.0%
TGCTTGGGATCCATCTGGAT+TGG - chr7.2:71401510-71401529 MS.gene99828:CDS 50.0%
TTCACCTGCGGATGAACCAA+CGG + chr7.2:71403688-71403707 None:intergenic 50.0%
! GAGTTTGCTTGGGATCCATC+TGG - chr7.2:71401505-71401524 MS.gene99828:CDS 50.0%
! TTGGGATCCATCTGGATTGG+AGG - chr7.2:71401513-71401532 MS.gene99828:CDS 50.0%
!! AAGCCCGGATCTTTTGACTC+GGG - chr7.2:71403738-71403757 MS.gene99828:CDS 50.0%
!! ACGAAGGAGTACTCGTACTC+CGG - chr7.2:71403879-71403898 MS.gene99828:CDS 50.0%
!! ACTCCTGTTGTGTTGGTTGG+AGG - chr7.2:71401793-71401812 MS.gene99828:CDS 50.0%
!! CAAGCCCGGATCTTTTGACT+CGG - chr7.2:71403737-71403756 MS.gene99828:CDS 50.0%
!! CCTACCACCTTTTTCACCTG+CGG + chr7.2:71403700-71403719 None:intergenic 50.0%
!! GGATTTGAGGCTTGGTGATC+AGG - chr7.2:71401561-71401580 MS.gene99828:CDS 50.0%
CCGCAGGTGAAAAAGGTGGT+AGG - chr7.2:71403697-71403716 MS.gene99828:CDS 55.0%
CCTATCAGCCATCCTATCCG+CGG - chr7.2:71403660-71403679 MS.gene99828:intron 55.0%
CTCTCTTCTGATGGCAAGCC+CGG - chr7.2:71403723-71403742 MS.gene99828:CDS 55.0%
GCAGCCATGAAGAGAGAAGG+TGG + chr7.2:71402323-71402342 None:intergenic 55.0%
GGTTCTGCCAACAATGCAGC+AGG - chr7.2:71401827-71401846 MS.gene99828:CDS 55.0%
TCTGAGGGCTACCAAAGTGC+AGG + chr7.2:71403458-71403477 None:intergenic 55.0%
TGGTTGGAGGTAAGCAGCAG+AGG - chr7.2:71401806-71401825 MS.gene99828:CDS 55.0%
! GGAGTACTCGTACTCCGGAA+AGG - chr7.2:71403884-71403903 MS.gene99828:CDS 55.0%
CCGCGGATAGGATGGCTGAT+AGG + chr7.2:71403663-71403682 None:intergenic 60.0%
CAACGGCACCGCGGATAGGA+TGG + chr7.2:71403671-71403690 None:intergenic 65.0%
CGGATGAACCAACGGCACCG+CGG + chr7.2:71403680-71403699 None:intergenic 65.0%
GAACCAACGGCACCGCGGAT+AGG + chr7.2:71403675-71403694 None:intergenic 65.0%
!! CATCCTATCCGCGGTGCCGT+TGG - chr7.2:71403669-71403688 MS.gene99828:intron 65.0%
!! GGTGCCGTTGGTTCATCCGC+AGG - chr7.2:71403681-71403700 MS.gene99828:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 71401487 71404046 71401487 ID=MS.gene99828
chr7.2 mRNA 71401487 71404046 71401487 ID=MS.gene99828.t1;Parent=MS.gene99828
chr7.2 exon 71403685 71404046 71403685 ID=MS.gene99828.t1.exon1;Parent=MS.gene99828.t1
chr7.2 CDS 71403685 71404046 71403685 ID=cds.MS.gene99828.t1;Parent=MS.gene99828.t1
chr7.2 exon 71403165 71403301 71403165 ID=MS.gene99828.t1.exon2;Parent=MS.gene99828.t1
chr7.2 CDS 71403165 71403301 71403165 ID=cds.MS.gene99828.t1;Parent=MS.gene99828.t1
chr7.2 exon 71401487 71402088 71401487 ID=MS.gene99828.t1.exon3;Parent=MS.gene99828.t1
chr7.2 CDS 71401487 71402088 71401487 ID=cds.MS.gene99828.t1;Parent=MS.gene99828.t1
Gene Sequence

>MS.gene99828

ATGGATTGGGATTGGAAAGAGTTTGCTTGGGATCCATCTGGATTGGAGGAGTTGAAAAATGAAGGAGATTCAATGGATTTGAGGCTTGGTGATCAGGCTTCTGATTCATTGGAGAAATCAGTTCATGATTCAGGAAAGGAAGATTCAAAAGCTGTGTCGTCGTCGTCACCTACTAGATCGTTGAAAAGAGCGCGTTTGCAAAATGGATCGCAGAATATGATTTGCTCGGTTGATGGATGTAACTCTGATCTTAGTGATTGTAGAGAGTATCATAAGCGTCATAGGGTGTGCGAAAAGCATTCCAAAACTCCTGTTGTGTTGGTTGGAGGTAAGCAGCAGAGGTTCTGCCAACAATGCAGCAGGTATATGAATTGAAGTTGTTTGTTTCGGGTTGATAACTTGTTGAATTTTGTTGTTTTAGTGTCATGTTTGAAGAAACAGCTTAATTAAACTCTTATAGCATTATTGTTTATCATTATAAGTGTTTATAATTTATGTATCTATGTGCACGTTATTTCTATAACAGTATAAAATAAAGTCAAACTGTTTTCATAAGCTATTCTGCAAAGAATATGAAAATGAGATGAAAACAACTTATGGACATGTCATAAGCTGTTTCCATATATAAGCTCTCTGAATATTCTAAAAATGGCTTATTTGAGTAGATAAGCTTAAAAATAACAATCCAAACATACCGAGTTTTGAGGTTGTTTTGTAATATGGGGTGTGGTTCAATTTTATGTAGGTTTCATTCACTTGCGGAGTTTGATGAGGTTAAGAGGAGTTGCAGGAAACGTCTTGATGGACATAATAAGCGCAGGAGGAAACCTCAGCCACCTTCTCTCTTCATGGCTGCTGAGAAGTTCATGTACAATTACAAAGGTTTGTGTCTTTTGTATCTCATGATTCTAAGATAGATGGCTTATTTCTGTCGATGTTTAACAAGATCTCAGTTTTATGATTGTCAAACCTGAGATGAAAGAGGGAATTATGACGAAGATATAACGTTTTTTATGGAAGAAGATACGCATGATGAATAAGTAAGAAATCACATATACATAACAATCCATAAGGTTTTGACAATAGAATTTAGAGTATCTCTCAAAATTTGTTTCAAATTTTTATTTACCATGAACTGAATTCTGAGCTAAGCTCAATAATTAGCTATAGTTATTTAGCGACTTTTGGATATACAACTTAATTAAAAACATACTTTATAAGTGCTTATTGTGAAAGAGTTTATGTTTAAGCTGATTCTACTATTGAAGAGGGAATGCATAAAAACTCTGGGTAAAGCTAAAATTAGCTTAGTGCAATAAGTTTCTAGGTGATGCTTAGCAAATATGTTAAAAGCAACTTAACAAACATGTCGTAATCTATTTTCATTTGCTTTTCCAAATACTTGCGCAAGTGTTTATGTCATAAGATAAGTTTAAATAAGCGCAAGTGTTTATGTCATAAGATAAGTTTAAATAAGCTCTTCCAATCCTCTCTCCAATCAAATTTAATGACAAAGAAAGTGACTTTCCGTCTTTATTTTCACAAGAAAGATGGTGGAGAAATATGAAACTTTGCTAATATTTCCCCATGGATTTCAATTGCAAAAAATGAATGCCCTGCATTTACACGATTAGTGCTTTAGTAACTGTTGGATTTAGATCGGACATTTTACAAAATTTGTTGATTTTGATCTTTTCGAAAATTCAAAATACTGTGCTGAAAATTCGCACGGTCCAATCTTGATCCAACAGTCACTGATGTGTTGTCTGCGTGAATTCATAATATCCCAACTGCAGAGAATCTTGATCCTTGATATCCGGCATGTTCAAACTTAACTAATATGCTTACTGCTGACATCTGTTTTTTTTTTATAGTCATAATTAATTACATTATTTTTGTTTTCCACAAGCACAGGTAATATGGAGTTTTAACACTGTTTTCTCTTTGTACAATTTTAGGACCTAGAATCCTGCACTTTGGTAGCCCTCAGACATATGTCAATCCTATTATGAGAAACATTTGGCCTGCAACTTCCATAACTGAAGCTGAATCTGGTTACGATCATCAAAGACTTCTGTATCGAATCGACAAACATAAACAGGATAAGGGACATCCTCATTGGCAAGAAATTCTCCCTAAGTCCGGCGATGTTAACAAAGCAGCATCTGGAACTCCTATCAGCCATCCTATCCGCGGTGCCGTTGGTTCATCCGCAGGTGAAAAAGGTGGTAGGAAGCTCTCTTCTGATGGCAAGCCCGGATCTTTTGACTCGGGTTGTGCTCTCTATCTTCTGTCAACACTTCAAACACAAAGCTCAGAGTTGAGTTTGATGCAGTCAAGCATCAACAACAGTCCAACACAATCTTCGTCTGGACCGTTGCATTTTGATGCTACGAAGGAGTACTCGTACTCCGGAAAGGCGAAAGATAAGCCGAATGGACCGGTATTTGTTCTAGATGGAAACACAACAAACCTTCATTGCAATGGGATGCTGCAAATGGGTCCTAATGGATTGATCGAAAATGATAACTCAGTGACTCTTCCATTCTTCTGGGAGTAG
Protein sequence

>MS.gene99828.t1

MDWDWKEFAWDPSGLEELKNEGDSMDLRLGDQASDSLEKSVHDSGKEDSKAVSSSSPTRSLKRARLQNGSQNMICSVDGCNSDLSDCREYHKRHRVCEKHSKTPVVLVGGKQQRFCQQCSRFHSLAEFDEVKRSCRKRLDGHNKRRRKPQPPSLFMAAEKFMYNYKGPRILHFGSPQTYVNPIMRNIWPATSITEAESGYDHQRLLYRIDKHKQDKGHPHWQEILPKSGDVNKAASGTPISHPIRGAVGSSAGEKGGRKLSSDGKPGSFDSGCALYLLSTLQTQSSELSLMQSSINNSPTQSSSGPLHFDATKEYSYSGKAKDKPNGPVFVLDGNTTNLHCNGMLQMGPNGLIENDNSVTLPFFWE