Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99844.t1 | XP_003622139.1 | 93.2 | 222 | 12 | 1 | 1 | 222 | 66 | 284 | 2.00E-98 | 368.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99844.t1 | Q9LSD5 | 58.9 | 209 | 63 | 6 | 1 | 203 | 94 | 285 | 1.5e-38 | 161.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99844.t1 | G7L283 | 93.2 | 222 | 12 | 1 | 1 | 222 | 66 | 284 | 1.4e-98 | 368.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene99844.t1 | TF | TCP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99844.t1 | MTR_7g028160 | 96.396 | 222 | 5 | 1 | 1 | 222 | 66 | 284 | 2.01e-154 | 429 |
| MS.gene99844.t1 | MTR_5g039600 | 57.292 | 96 | 24 | 3 | 1 | 79 | 83 | 178 | 3.31e-25 | 102 |
| MS.gene99844.t1 | MTR_2g006150 | 75.000 | 64 | 10 | 1 | 1 | 58 | 146 | 209 | 1.51e-24 | 100 |
| MS.gene99844.t1 | MTR_2g006150 | 75.000 | 64 | 10 | 1 | 1 | 58 | 146 | 209 | 1.74e-24 | 100 |
| MS.gene99844.t1 | MTR_8g033070 | 70.968 | 62 | 18 | 0 | 1 | 62 | 96 | 157 | 2.24e-24 | 99.0 |
| MS.gene99844.t1 | MTR_4g108370 | 89.362 | 47 | 5 | 0 | 1 | 47 | 113 | 159 | 8.10e-24 | 98.6 |
| MS.gene99844.t1 | MTR_1g101810 | 70.968 | 62 | 18 | 0 | 1 | 62 | 80 | 141 | 1.01e-23 | 97.1 |
| MS.gene99844.t1 | MTR_1g063870 | 85.106 | 47 | 7 | 0 | 1 | 47 | 65 | 111 | 1.51e-23 | 94.0 |
| MS.gene99844.t1 | MTR_1g101810 | 70.968 | 62 | 18 | 0 | 1 | 62 | 110 | 171 | 1.54e-23 | 97.1 |
| MS.gene99844.t1 | MTR_1g038650 | 62.162 | 74 | 28 | 0 | 1 | 74 | 48 | 121 | 3.65e-23 | 93.6 |
| MS.gene99844.t1 | MTR_1g114380 | 83.333 | 48 | 8 | 0 | 1 | 48 | 49 | 96 | 2.30e-22 | 92.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99844.t1 | AT3G27010 | 56.061 | 198 | 64 | 7 | 1 | 192 | 94 | 274 | 2.60e-49 | 164 |
| MS.gene99844.t1 | AT3G27010 | 56.061 | 198 | 64 | 7 | 1 | 192 | 94 | 274 | 2.60e-49 | 164 |
| MS.gene99844.t1 | AT1G58100 | 75.000 | 64 | 10 | 1 | 1 | 58 | 76 | 139 | 2.56e-25 | 102 |
| MS.gene99844.t1 | AT5G41030 | 35.437 | 206 | 69 | 11 | 1 | 204 | 84 | 227 | 1.03e-24 | 98.2 |
| MS.gene99844.t1 | AT2G45680 | 64.706 | 68 | 24 | 0 | 1 | 68 | 91 | 158 | 4.95e-24 | 98.2 |
| MS.gene99844.t1 | AT3G47620 | 91.489 | 47 | 4 | 0 | 1 | 47 | 133 | 179 | 6.16e-24 | 99.4 |
| MS.gene99844.t1 | AT1G69690 | 85.417 | 48 | 7 | 0 | 1 | 48 | 68 | 115 | 6.42e-24 | 97.4 |
| MS.gene99844.t1 | AT1G35560 | 73.438 | 64 | 11 | 1 | 1 | 58 | 71 | 134 | 7.57e-24 | 97.4 |
| MS.gene99844.t1 | AT1G72010 | 87.234 | 47 | 6 | 0 | 1 | 47 | 79 | 125 | 1.93e-23 | 97.1 |
| MS.gene99844.t1 | AT5G51910 | 65.625 | 64 | 22 | 0 | 1 | 64 | 73 | 136 | 2.24e-23 | 95.5 |
| MS.gene99844.t1 | AT5G51910 | 65.625 | 64 | 22 | 0 | 1 | 64 | 73 | 136 | 2.24e-23 | 95.5 |
| MS.gene99844.t1 | AT5G08330 | 72.881 | 59 | 16 | 0 | 1 | 59 | 47 | 105 | 2.56e-23 | 94.4 |
| MS.gene99844.t1 | AT5G23280 | 83.333 | 48 | 8 | 0 | 1 | 48 | 55 | 102 | 1.37e-22 | 92.4 |
| MS.gene99844.t1 | AT2G37000 | 84.091 | 44 | 7 | 0 | 1 | 44 | 59 | 102 | 4.22e-19 | 82.0 |
| MS.gene99844.t1 | AT3G45150 | 58.140 | 43 | 18 | 0 | 1 | 43 | 33 | 75 | 1.08e-11 | 61.6 |
Find 56 sgRNAs with CRISPR-Local
Find 64 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGATGCCTGGTTTGGAATT+AGG | 0.117190 | 7.2:+71856964 | MS.gene99844:CDS |
| ATGTTAGTTCTACAAGTTTA+TGG | 0.160920 | 7.2:+71856746 | MS.gene99844:CDS |
| GATGGGCCTGGTGGGCTTGA+TGG | 0.187359 | 7.2:-71856705 | None:intergenic |
| CAAGGTTCATCTTTGTCTTC+TGG | 0.222188 | 7.2:+71856646 | MS.gene99844:CDS |
| TATTATTATTCTGCTGATTC+TGG | 0.237820 | 7.2:-71857080 | None:intergenic |
| CATCATCAAGTTGGTGGATA+TGG | 0.242402 | 7.2:+71856772 | MS.gene99844:CDS |
| TATGCCAACAGGAACAAATT+TGG | 0.247795 | 7.2:+71856894 | MS.gene99844:CDS |
| AGTTGAATGATAGGAATAAT+TGG | 0.264011 | 7.2:+71856674 | MS.gene99844:CDS |
| AGATGCCTGGTTTGGAATTA+GGG | 0.264298 | 7.2:+71856965 | MS.gene99844:CDS |
| ATGGGCCTGGTGGGCTTGAT+GGG | 0.294523 | 7.2:-71856704 | None:intergenic |
| GCTTCAGCTTTAGCTTCTTC+TGG | 0.304919 | 7.2:+71856607 | MS.gene99844:CDS |
| CCTGTTGCAGCTATGATTGA+TGG | 0.306906 | 7.2:-71856568 | None:intergenic |
| GAATTGTGAGAATCATCTTT+AGG | 0.322739 | 7.2:-71857105 | None:intergenic |
| CTTGATGGGCCTGATGGGCC+TGG | 0.347126 | 7.2:-71856717 | None:intergenic |
| TTTCAGCTAACAAGAGAGTT+AGG | 0.376457 | 7.2:+71856496 | MS.gene99844:CDS |
| ATGGGCCTGATGGGCCTGGT+GGG | 0.388474 | 7.2:-71856713 | None:intergenic |
| CTGGTTTGCAGTTGAATGAT+AGG | 0.398808 | 7.2:+71856665 | MS.gene99844:CDS |
| ATGCCAACAGGAACAAATTT+GGG | 0.405289 | 7.2:+71856895 | MS.gene99844:CDS |
| TCAAGCCCACCAGGCCCATC+AGG | 0.414777 | 7.2:+71856708 | MS.gene99844:CDS |
| ATCATAGCTGCAACAGGAAC+AGG | 0.443120 | 7.2:+71856574 | MS.gene99844:CDS |
| ACTCAGATTTATCAGCAGAT+TGG | 0.447988 | 7.2:+71857033 | MS.gene99844:CDS |
| TCTGGATTTGATATGCCAAC+AGG | 0.449552 | 7.2:+71856883 | MS.gene99844:CDS |
| ACTCCCAAATTTGTTCCTGT+TGG | 0.452759 | 7.2:-71856898 | None:intergenic |
| GTTGAATGATAGGAATAATT+GGG | 0.466051 | 7.2:+71856675 | MS.gene99844:CDS |
| TTTCATCAATCATCATCATC+TGG | 0.469042 | 7.2:+71856796 | MS.gene99844:CDS |
| AAATCAGCAGATGCCTGGTT+TGG | 0.470684 | 7.2:+71856957 | MS.gene99844:CDS |
| AACTAACATGATGACCTTGA+TGG | 0.471715 | 7.2:-71856732 | None:intergenic |
| ATGACCTTGATGGGCCTGAT+GGG | 0.473600 | 7.2:-71856722 | None:intergenic |
| CCATCAATCATAGCTGCAAC+AGG | 0.479395 | 7.2:+71856568 | MS.gene99844:CDS |
| GATGGGCCTGATGGGCCTGG+TGG | 0.480862 | 7.2:-71856714 | None:intergenic |
| TTGTCACAAGATGGACATAT+TGG | 0.480930 | 7.2:+71856988 | MS.gene99844:CDS |
| GAATTAGGGTTGTCACAAGA+TGG | 0.488895 | 7.2:+71856979 | MS.gene99844:CDS |
| ACTGTTGGTGAAAATAATAG+TGG | 0.505174 | 7.2:+71856835 | MS.gene99844:CDS |
| TGGCCTCATCATCAAGTTGG+TGG | 0.511204 | 7.2:+71856766 | MS.gene99844:CDS |
| GGTGCAAATCAGCAGATGCC+TGG | 0.519917 | 7.2:+71856952 | MS.gene99844:CDS |
| TCAGACCCATCAAGCCCACC+AGG | 0.525810 | 7.2:+71856699 | MS.gene99844:CDS |
| CAGATGGTGAAACAATTCAA+TGG | 0.529264 | 7.2:+71856527 | MS.gene99844:CDS |
| AGATTGGTCAAAATCAAACT+AGG | 0.530078 | 7.2:+71857049 | MS.gene99844:CDS |
| CAGGCCCATCAGGCCCATCA+AGG | 0.530139 | 7.2:+71856718 | MS.gene99844:CDS |
| ATTCTTGCTGCACATAAAGC+TGG | 0.536084 | 7.2:-71856472 | None:intergenic |
| AACAGGAACAAATTTGGGAG+TGG | 0.545963 | 7.2:+71856900 | MS.gene99844:CDS |
| GGTTTAGTAGCTACTACTGT+TGG | 0.551441 | 7.2:+71856820 | MS.gene99844:CDS |
| GATGACCTTGATGGGCCTGA+TGG | 0.557322 | 7.2:-71856723 | None:intergenic |
| ACTAACATGATGACCTTGAT+GGG | 0.557914 | 7.2:-71856731 | None:intergenic |
| GGAACAAATTTGGGAGTGGG+AGG | 0.568972 | 7.2:+71856904 | MS.gene99844:CDS |
| CATCAATCATCATCATCTGG+TGG | 0.574348 | 7.2:+71856799 | MS.gene99844:CDS |
| GAAGAAGCTAAAGCTGAAGC+TGG | 0.576280 | 7.2:-71856604 | None:intergenic |
| GGTAATACTTTGACACCACA+AGG | 0.588387 | 7.2:+71856628 | MS.gene99844:CDS |
| ACAGGAACAAATTTGGGAGT+GGG | 0.594534 | 7.2:+71856901 | MS.gene99844:CDS |
| GATTGGTCAAAATCAAACTA+GGG | 0.595101 | 7.2:+71857050 | MS.gene99844:CDS |
| GAACAAATTTGGGAGTGGGA+GGG | 0.598189 | 7.2:+71856905 | MS.gene99844:CDS |
| TTATGGCCTCATCATCAAGT+TGG | 0.605767 | 7.2:+71856763 | MS.gene99844:CDS |
| GAGTTAGGTCATAAATCAGA+TGG | 0.610512 | 7.2:+71856511 | MS.gene99844:CDS |
| TATCCACCAACTTGATGATG+AGG | 0.610804 | 7.2:-71856769 | None:intergenic |
| GACAACCCTAATTCCAAACC+AGG | 0.628289 | 7.2:-71856970 | None:intergenic |
| GAAGACAAAGATGAACCTTG+TGG | 0.735014 | 7.2:-71856643 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | GAAATTATTTTCAGAAAATT+GGG | + | chr7.2:71856857-71856876 | MS.gene99844:CDS | 15.0% |
| !!! | ATGAGTTTTACATCAATTTT+GGG | + | chr7.2:71856928-71856947 | MS.gene99844:CDS | 20.0% |
| !!! | GGAAATTATTTTCAGAAAAT+TGG | + | chr7.2:71856856-71856875 | MS.gene99844:CDS | 20.0% |
| ! | AGTTGAATGATAGGAATAAT+TGG | + | chr7.2:71856674-71856693 | MS.gene99844:CDS | 25.0% |
| ! | GTTGAATGATAGGAATAATT+GGG | + | chr7.2:71856675-71856694 | MS.gene99844:CDS | 25.0% |
| !! | GATGAGTTTTACATCAATTT+TGG | + | chr7.2:71856927-71856946 | MS.gene99844:CDS | 25.0% |
| !! | TGAGTTTTACATCAATTTTG+GGG | + | chr7.2:71856929-71856948 | MS.gene99844:CDS | 25.0% |
| !!! | ATGTTAGTTCTACAAGTTTA+TGG | + | chr7.2:71856746-71856765 | MS.gene99844:CDS | 25.0% |
| !!! | TATTATTATTCTGCTGATTC+TGG | - | chr7.2:71857083-71857102 | None:intergenic | 25.0% |
| GAATTGTGAGAATCATCTTT+AGG | - | chr7.2:71857108-71857127 | None:intergenic | 30.0% | |
| TTTCATCAATCATCATCATC+TGG | + | chr7.2:71856796-71856815 | MS.gene99844:CDS | 30.0% | |
| ! | ACTGTTGGTGAAAATAATAG+TGG | + | chr7.2:71856835-71856854 | MS.gene99844:CDS | 30.0% |
| ! | AGATTGGTCAAAATCAAACT+AGG | + | chr7.2:71857049-71857068 | MS.gene99844:CDS | 30.0% |
| ! | AGTTTTACATCAATTTTGGG+GGG | + | chr7.2:71856931-71856950 | MS.gene99844:CDS | 30.0% |
| ! | GAGTTTTACATCAATTTTGG+GGG | + | chr7.2:71856930-71856949 | MS.gene99844:CDS | 30.0% |
| ! | GATTGGTCAAAATCAAACTA+GGG | + | chr7.2:71857050-71857069 | MS.gene99844:CDS | 30.0% |
| ! | TTTCAGAAAATTGGGTTTTC+TGG | + | chr7.2:71856865-71856884 | MS.gene99844:CDS | 30.0% |
| AACTAACATGATGACCTTGA+TGG | - | chr7.2:71856735-71856754 | None:intergenic | 35.0% | |
| ACTAACATGATGACCTTGAT+GGG | - | chr7.2:71856734-71856753 | None:intergenic | 35.0% | |
| ACTCAGATTTATCAGCAGAT+TGG | + | chr7.2:71857033-71857052 | MS.gene99844:CDS | 35.0% | |
| ATGCCAACAGGAACAAATTT+GGG | + | chr7.2:71856895-71856914 | MS.gene99844:CDS | 35.0% | |
| CAGATGGTGAAACAATTCAA+TGG | + | chr7.2:71856527-71856546 | MS.gene99844:CDS | 35.0% | |
| GAGTTAGGTCATAAATCAGA+TGG | + | chr7.2:71856511-71856530 | MS.gene99844:CDS | 35.0% | |
| TATGCCAACAGGAACAAATT+TGG | + | chr7.2:71856894-71856913 | MS.gene99844:CDS | 35.0% | |
| TTGTCACAAGATGGACATAT+TGG | + | chr7.2:71856988-71857007 | MS.gene99844:CDS | 35.0% | |
| TTTCAGCTAACAAGAGAGTT+AGG | + | chr7.2:71856496-71856515 | MS.gene99844:CDS | 35.0% | |
| AACAGGAACAAATTTGGGAG+TGG | + | chr7.2:71856900-71856919 | MS.gene99844:CDS | 40.0% | |
| ACAGGAACAAATTTGGGAGT+GGG | + | chr7.2:71856901-71856920 | MS.gene99844:CDS | 40.0% | |
| ATTCTTGCTGCACATAAAGC+TGG | - | chr7.2:71856475-71856494 | None:intergenic | 40.0% | |
| CAAGGTTCATCTTTGTCTTC+TGG | + | chr7.2:71856646-71856665 | MS.gene99844:CDS | 40.0% | |
| CATCAATCATCATCATCTGG+TGG | + | chr7.2:71856799-71856818 | MS.gene99844:CDS | 40.0% | |
| GAAGACAAAGATGAACCTTG+TGG | - | chr7.2:71856646-71856665 | None:intergenic | 40.0% | |
| GAATTAGGGTTGTCACAAGA+TGG | + | chr7.2:71856979-71856998 | MS.gene99844:CDS | 40.0% | |
| GGTAATACTTTGACACCACA+AGG | + | chr7.2:71856628-71856647 | MS.gene99844:CDS | 40.0% | |
| GGTTTAGTAGCTACTACTGT+TGG | + | chr7.2:71856820-71856839 | MS.gene99844:CDS | 40.0% | |
| TCTGGATTTGATATGCCAAC+AGG | + | chr7.2:71856883-71856902 | MS.gene99844:CDS | 40.0% | |
| TTATGGCCTCATCATCAAGT+TGG | + | chr7.2:71856763-71856782 | MS.gene99844:CDS | 40.0% | |
| ! | ACTCCCAAATTTGTTCCTGT+TGG | - | chr7.2:71856901-71856920 | None:intergenic | 40.0% |
| ! | CTGGTTTGCAGTTGAATGAT+AGG | + | chr7.2:71856665-71856684 | MS.gene99844:CDS | 40.0% |
| !! | AGATGCCTGGTTTGGAATTA+GGG | + | chr7.2:71856965-71856984 | MS.gene99844:CDS | 40.0% |
| !! | CATCATCAAGTTGGTGGATA+TGG | + | chr7.2:71856772-71856791 | MS.gene99844:CDS | 40.0% |
| !! | TATCCACCAACTTGATGATG+AGG | - | chr7.2:71856772-71856791 | None:intergenic | 40.0% |
| AAATCAGCAGATGCCTGGTT+TGG | + | chr7.2:71856957-71856976 | MS.gene99844:CDS | 45.0% | |
| ATCATAGCTGCAACAGGAAC+AGG | + | chr7.2:71856574-71856593 | MS.gene99844:CDS | 45.0% | |
| CCATCAATCATAGCTGCAAC+AGG | + | chr7.2:71856568-71856587 | MS.gene99844:CDS | 45.0% | |
| CCTGTTGCAGCTATGATTGA+TGG | - | chr7.2:71856571-71856590 | None:intergenic | 45.0% | |
| GAACAAATTTGGGAGTGGGA+GGG | + | chr7.2:71856905-71856924 | MS.gene99844:CDS | 45.0% | |
| GAAGAAGCTAAAGCTGAAGC+TGG | - | chr7.2:71856607-71856626 | None:intergenic | 45.0% | |
| GACAACCCTAATTCCAAACC+AGG | - | chr7.2:71856973-71856992 | None:intergenic | 45.0% | |
| ! | GCTTCAGCTTTAGCTTCTTC+TGG | + | chr7.2:71856607-71856626 | MS.gene99844:CDS | 45.0% |
| !! | CAGATGCCTGGTTTGGAATT+AGG | + | chr7.2:71856964-71856983 | MS.gene99844:CDS | 45.0% |
| GGAACAAATTTGGGAGTGGG+AGG | + | chr7.2:71856904-71856923 | MS.gene99844:CDS | 50.0% | |
| ! | ATGACCTTGATGGGCCTGAT+GGG | - | chr7.2:71856725-71856744 | None:intergenic | 50.0% |
| !! | TGGCCTCATCATCAAGTTGG+TGG | + | chr7.2:71856766-71856785 | MS.gene99844:CDS | 50.0% |
| GGTGCAAATCAGCAGATGCC+TGG | + | chr7.2:71856952-71856971 | MS.gene99844:CDS | 55.0% | |
| ! | GATGACCTTGATGGGCCTGA+TGG | - | chr7.2:71856726-71856745 | None:intergenic | 55.0% |
| TCAGACCCATCAAGCCCACC+AGG | + | chr7.2:71856699-71856718 | MS.gene99844:CDS | 60.0% | |
| !! | ATGGGCCTGGTGGGCTTGAT+GGG | - | chr7.2:71856707-71856726 | None:intergenic | 60.0% |
| CAGGCCCATCAGGCCCATCA+AGG | + | chr7.2:71856718-71856737 | MS.gene99844:CDS | 65.0% | |
| TCAAGCCCACCAGGCCCATC+AGG | + | chr7.2:71856708-71856727 | MS.gene99844:CDS | 65.0% | |
| ! | ATGGGCCTGATGGGCCTGGT+GGG | - | chr7.2:71856716-71856735 | None:intergenic | 65.0% |
| ! | CTTGATGGGCCTGATGGGCC+TGG | - | chr7.2:71856720-71856739 | None:intergenic | 65.0% |
| !! | GATGGGCCTGGTGGGCTTGA+TGG | - | chr7.2:71856708-71856727 | None:intergenic | 65.0% |
| ! | GATGGGCCTGATGGGCCTGG+TGG | - | chr7.2:71856717-71856736 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 71856469 | 71857137 | 71856469 | ID=MS.gene99844 |
| chr7.2 | mRNA | 71856469 | 71857137 | 71856469 | ID=MS.gene99844.t1;Parent=MS.gene99844 |
| chr7.2 | exon | 71856469 | 71857137 | 71856469 | ID=MS.gene99844.t1.exon1;Parent=MS.gene99844.t1 |
| chr7.2 | CDS | 71856469 | 71857137 | 71856469 | ID=cds.MS.gene99844.t1;Parent=MS.gene99844.t1 |
| Gene Sequence |
| Protein sequence |
