AlfalfaGEDB | MS.gene99998

M. sativa cultivar XinJiangDaYe / MS.gene99998

KEGG: K10875
GO: GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0008144 GO:0017076 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0097159 GO:0097367 GO:1901265 GO:1901363
PFAM: PF00176.26

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99998.t1	RHN80703.1	79.2	595	87	4	1	562	87	677	6.90E-260	906.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99998.t1	F4K493	31.0	749	314	16	4	561	98	834	1.9e-77	291.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99998.t1	A0A072VN36	79.2	595	87	4	1	562	100	690	5.0e-260	906.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene99998.t1	TR	SNF2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99998.t1	MTR_1g081750	79.160	595	87	4	1	562	100	690	0.0	944
MS.gene99998.t1	MTR_7g026650	51.623	647	219	17	2	561	87	726	0.0	570
MS.gene99998.t1	MTR_3g071860	50.826	484	175	12	142	562	356	839	2.92e-136	427
MS.gene99998.t1	MTR_3g071860	66.667	150	47	2	1	147	102	251	1.14e-55	203
MS.gene99998.t1	MTR_5g083300	47.429	350	144	5	251	561	526	874	3.66e-87	294
MS.gene99998.t1	MTR_5g083300	43.373	166	75	5	3	161	104	257	5.98e-25	110
MS.gene99998.t1	MTR_5g083300	47.429	350	144	5	251	561	563	911	3.69e-87	295
MS.gene99998.t1	MTR_5g083300	43.373	166	75	5	3	161	141	294	5.16e-25	110
MS.gene99998.t1	MTR_7g026620	42.506	407	140	14	140	532	11	337	1.30e-77	258
MS.gene99998.t1	MTR_7g450860	34.892	278	153	7	303	563	596	862	4.17e-42	163

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene99998.t1	AT5G20420	42.693	349	161	6	252	561	486	834	8.44e-77	265
MS.gene99998.t1	AT5G20420	38.272	162	87	4	3	163	97	246	4.65e-23	104
MS.gene99998.t1	AT3G42670	42.286	350	162	7	251	561	480	828	5.81e-75	259
MS.gene99998.t1	AT3G42670	41.985	131	64	4	3	132	97	216	7.22e-19	91.7
MS.gene99998.t1	AT3G42670	42.286	350	162	7	251	561	480	828	5.81e-75	259
MS.gene99998.t1	AT3G42670	41.985	131	64	4	3	132	97	216	7.22e-19	91.7
MS.gene99998.t1	AT3G42670	42.286	350	162	7	251	561	480	828	5.81e-75	259
MS.gene99998.t1	AT3G42670	41.985	131	64	4	3	132	97	216	7.22e-19	91.7
MS.gene99998.t1	AT3G42670	51.406	249	115	4	318	561	583	830	1.21e-74	258
MS.gene99998.t1	AT3G42670	41.985	131	64	4	3	132	97	216	7.41e-19	91.7

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Find 86 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATCACACTGAAAGCGCA+TGG	0.616805	5.2:-41430937	MS.gene99998:CDS
AAAATGGAATGTTCCCATGC+CGG	0.580266	5.2:-41431160	MS.gene99998:CDS
AAAGAAAACGTATTGGAAAG+CGG	0.682656	5.2:-41432448	MS.gene99998:CDS
AAAGAACAGGATCATAGAGG+TGG	0.669391	5.2:-41432589	MS.gene99998:CDS
AACTTCAAATCCTGTGATGA+TGG	0.524328	5.2:-41432880	MS.gene99998:CDS
AAGAAAAGAGATGGTGAGAA+AGG	0.416344	5.2:+41431269	None:intergenic
AAGAAAGAGGATAATGTGAT+TGG	0.408479	5.2:-41433189	MS.gene99998:CDS
AAGATTGTGTATGAAGTTAT+GGG	0.424794	5.2:-41432940	MS.gene99998:CDS
AATAAGGAAAATCAGTTGAC+TGG	0.505533	5.2:-41432838	MS.gene99998:CDS
ACACCCTATTGTTCTTGTCA+TGG	0.436213	5.2:-41431004	MS.gene99998:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAAAATCTGACACAAC+TGG	-	chr5.2:41432918-41432937	MS.gene99998:CDS	30.0%
AAAGAAAACGTATTGGAAAG+CGG	-	chr5.2:41431800-41431819	MS.gene99998:intron	30.0%
AACTCTTAAATACCATAGCA+GGG	-	chr5.2:41432212-41432231	MS.gene99998:intron	30.0%
AAGAAAGAGGATAATGTGAT+TGG	-	chr5.2:41431059-41431078	MS.gene99998:CDS	30.0%
AATAAGGAAAATCAGTTGAC+TGG	-	chr5.2:41431410-41431429	MS.gene99998:CDS	30.0%
AATTTATATCCAATGGTAGG+TGG	-	chr5.2:41432653-41432672	MS.gene99998:CDS	30.0%
ACATCAAGTTATCTTCTTGA+TGG	-	chr5.2:41431884-41431903	MS.gene99998:intron	30.0%
ACTGTCAACAAAATCATAGT+CGG	-	chr5.2:41432020-41432039	MS.gene99998:intron	30.0%
ATGTGAACATGATTATCGAT+TGG	-	chr5.2:41432286-41432305	MS.gene99998:intron	30.0%
CAAAGAAAATGACAGTGTTT+GGG	-	chr5.2:41432794-41432813	MS.gene99998:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	41430933	41433337	41430933	ID=MS.gene99998
chr5.2	mRNA	41430933	41433337	41430933	ID=MS.gene99998.t1;Parent=MS.gene99998
chr5.2	exon	41432441	41433337	41432441	ID=MS.gene99998.t1.exon1;Parent=MS.gene99998.t1
chr5.2	CDS	41432441	41433337	41432441	ID=cds.MS.gene99998.t1;Parent=MS.gene99998.t1
chr5.2	exon	41431888	41432037	41431888	ID=MS.gene99998.t1.exon2;Parent=MS.gene99998.t1
chr5.2	CDS	41431888	41432037	41431888	ID=cds.MS.gene99998.t1;Parent=MS.gene99998.t1
chr5.2	exon	41430933	41431598	41430933	ID=MS.gene99998.t1.exon3;Parent=MS.gene99998.t1
chr5.2	CDS	41430933	41431598	41430933	ID=cds.MS.gene99998.t1;Parent=MS.gene99998.t1

Gene Sequence

>MS.gene99998

ATGTCGACTGATGCTAGAGTGAATTGCATACACAAAAAACCTCACAAATATAATAAATGTTCGTGTCGATTTCATGTAAAGTTTTATGATGATCAAGGTTCACATGGCACAGAAATAAAAACTCTGAAGAAAGAGGATAATGTGATTGGAATTGAGAAAATTTTCATCCTCCAAAAACTTCAACGAAATAACTGTGAAGGATCGTTACGAGAAAATGATTGTTCAGAAGTTAATTCTTTTAGGTGGGATTCATCGGAAGACCGCTCTTCATTGCCACAATCTAGGTTACTTTCCGGGAAGTTTTTGTCTGATCTTTCCTGGTTTGTTACCGCGTCATCTTTAAAGGTTTCATATGTCATAAGATCGGTTCAAAACAAGATTGTGTATGAAGTTATGGGCAATTATCCTAATATTAGTGGTCCTATGAATGTTTTGAACTTCAAATCCTGTGATGATGGTAAGAGTGGTCCAAAGGTTAATAAGGAAAATCAGTTGACTGGTGCTAAAGAAAAGAACGCTGTTGATGTGTTAAATCATGAGCCATACAATGAGTCTCCATTATCATGTGATGAAGAAGGTGGTTATATTGTTTTTCGGGACATGTTAACAATTTCTGCACGAAGAAGGCGGATCTGTAAGGAGCATATGGATTCGATAATTTTATTGGAAGCGATACACTCTAAAGAAGATGTTCAAAAGAAAACGACAAATAGTATCGATGATCATAAAGAACAGGATCATAGAGGTGGTAGAACGTTAAATGCTGATACCTGCGAGGAAGTAATTGATGCTTACATGGATAATTTTGATAGCCTACCATCTGAAGAAGATCCAACTATCAGTGAAGAAAGACGGTTTGAGCAGAAGAAAGAAAACGTATTGGAAAGCGGTGGTGAGGTAGAAAATCCTGATGATTTGACTGACATATAGAAAGAAATGGATACAGCTTTGACATCAAGTTATCTTCTTGATGGAAATGAGGTGTGTGACTAAAATTTGGTTTCCCATTTCTTGTCTTTTACTTGTCATGTGATATCATATGTTTTTTAGTTTAATTTTGATATCCTATCTGTATAAAATGTTTTACACTGTCAACAAAATCATAGTCGGTCATATAATTGACCGTTGAACCATTTATGGTTGAAGTCAACTATGTTTAATGATATGACAATTATGATTTATAGGCAGTGTACATAAGTTTTACACTCGCAATATATGATTTAAATACTTTGTTTTTTGGTTTTATCTTCTATTTTAAGGCTAAAGGCTCATTATGTTCAACTCTTAAATACCATAGCAGGGTGCAAATGCTGATGAGGTTTTAGCTGACACAAATAAAGAATCCAAGGAAACATGTGAACATGATTATCGATTGGATGAACAAATTGGAATATACTGCCGTACATGCGGCTTTGTGAAAACTGAGAAACGATACATTTCTGAACCCATTGTAAGTCAAGTAAAAACTTAAGAATAATCTATGCATGTTTGAACTTGCAATGAATTTGATAAAATCACAGTGCGCCACTGCGATTTTCCAAAAGTTACACTTTGAAACTTCACAAAATCACTATGCAACTGTGATTTTACCAGATCCACCATGATTTCAAACTTGTAATTATACATGTAACTTTAGTTCCTTATTTGGTTGGATATGTTTTCTATTGATGTTAACTATATTCTCATTTTGCTTGTTACACGTTTTCTCATTTGGTTGTCTAATTTATATCCAATGGTAGGTGGAAAGGCTAAAATGGTACGAACAGAAGAAACAAAGCAGTGAACATACGGAACAAGTTGATGAAGATGATAATAATGATATGTTTTCAACTGATGCTACTAATCTTGGTGAACCAATATCCAAAGAAAATGACAGTGTTTGGGAATTGATTCCTGAACTCAAAGAAAACATGCATGCACACCAGAAAAAAGCTTTTCAATTTCTTTGGAAAAACATTGCAGGGTCTATGGAGCAATCACACATGAAAGAAAAATCTGACACAACTGGTGGCTGTGTTATATCTCATGCTCCTGGAGCTGGAAAAACCTTTCTCACCATCTCTTTTCTTGTAAGTTATTTGAAATTATTTCTAGAGAAAAGACCATTAGTCCTAGCTCCAAAGACCACACTCTACACATGGCAAAAAGAGTTCGAAAAATGGAATGTTCCCATGCCGGTGTATCTTATCCACATCTGTCGAAGCCAGAGACATTCGACGACACCAAAATCAATGGTTCTACCTGGAGTTCCAAATTCTAATGGTATCAAGCATGATTTGGATTGTATACAAAAGATCAAAAGCAGGAATTCACACCCTATTGTTCTTGTCATGGGCTATTCTTCATTTTTAGCCTTAATGAGAACAGAAGATAAAAAAAAAATCACACTGAAAGCGCATGGCTAA

Protein sequence

>MS.gene99998.t1

MSTDARVNCIHKKPHKYNKCSCRFHVKFYDDQGSHGTEIKTLKKEDNVIGIEKIFILQKLQRNNCEGSLRENDCSEVNSFRWDSSEDRSSLPQSRLLSGKFLSDLSWFVTASSLKVSYVIRSVQNKIVYEVMGNYPNISGPMNVLNFKSCDDGKSGPKVNKENQLTGAKEKNAVDVLNHEPYNESPLSCDEEGGYIVFRDMLTISARRRRICKEHMDSIILLEAIHSKEDVQKKTTNSIDDHKEQDHRGGRTLNADTCEEVIDAYMDNFDSLPSEEDPTISEERRFEQKKENVLESGGEGANADEVLADTNKESKETCEHDYRLDEQIGIYCRTCGFVKTEKRYISEPIVERLKWYEQKKQSSEHTEQVDEDDNNDMFSTDATNLGEPISKENDSVWELIPELKENMHAHQKKAFQFLWKNIAGSMEQSHMKEKSDTTGGCVISHAPGAGKTFLTISFLVSYLKLFLEKRPLVLAPKTTLYTWQKEFEKWNVPMPVYLIHICRSQRHSTTPKSMVLPGVPNSNGIKHDLDCIQKIKSRNSHPIVLVMGYSSFLALMRTEDKKKITLKAHG