AlfalfaGEDB | MsG0080048017.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048017.01

KEGG: -
GO: GO:0046983
PFAM: PF00010

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048017.01.T01	XP_003623323.1	86.928	153	19	1	1	152	1	153	2.54E-74	229

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048017.01.T01	Q9M041	65.06	83	29	0	15	97	26	108	1.93E-30	118

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048017.01.T01	G7KSC0	86.928	153	19	1	1	152	1	153	1.21e-74	229

TFs/TRs:

Gene ID	Type	Classification
MsG0080048017.01.T01	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048017.01.T01	MTR_7g069590	86.928	153	19	1	1	152	1	153	3.08e-78	229
MsG0080048017.01.T01	MTR_3g101810	61.765	68	26	0	18	85	163	230	1.43e-24	95.9
MsG0080048017.01.T01	MTR_8g093500	62.121	66	25	0	22	87	110	175	5.08e-23	92.0
MsG0080048017.01.T01	MTR_5g017210	61.111	72	28	0	18	89	129	200	4.89e-18	78.2
MsG0080048017.01.T01	MTR_5g079500	66.038	53	18	0	33	85	225	277	5.56e-18	79.0
MsG0080048017.01.T01	MTR_5g005110	43.617	94	44	2	28	112	226	319	2.28e-17	77.4
MsG0080048017.01.T01	MTR_1g018090	51.515	66	28	1	22	87	274	335	2.30e-17	77.8
MsG0080048017.01.T01	MTR_1g106460	52.941	68	29	1	20	87	235	299	6.38e-17	76.3
MsG0080048017.01.T01	MTR_1g106470	43.529	85	39	2	23	99	194	277	3.26e-16	73.9
MsG0080048017.01.T01	MTR_2g100490	48.276	87	43	1	22	108	286	370	3.79e-16	74.3
MsG0080048017.01.T01	MTR_8g015450	56.757	74	32	0	23	96	325	398	3.85e-16	74.7
MsG0080048017.01.T01	MTR_3g103030	50.769	65	28	1	23	87	230	290	2.24e-15	72.0
MsG0080048017.01.T01	MTR_4g131160	37.931	87	50	1	3	85	172	258	5.87e-13	65.5
MsG0080048017.01.T01	MTR_4g131160	37.931	87	50	1	3	85	169	255	5.96e-13	65.5
MsG0080048017.01.T01	MTR_1g106480	43.662	71	33	2	44	107	87	157	2.95e-12	61.2
MsG0080048017.01.T01	MTR_1g080890	37.838	74	46	0	12	85	179	252	1.24e-11	61.6
MsG0080048017.01.T01	MTR_1g060700	44.068	59	33	0	36	94	357	415	1.36e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048017.01.T01	AT5G01305	65.060	83	29	0	15	97	26	108	9.88e-35	119
MsG0080048017.01.T01	AT3G21330	55.714	70	31	0	22	91	265	334	5.16e-22	90.5
MsG0080048017.01.T01	AT5G43175	39.024	123	59	3	2	109	102	223	5.38e-21	85.5
MsG0080048017.01.T01	AT4G00120	54.688	64	29	0	22	85	108	171	5.38e-20	82.4
MsG0080048017.01.T01	AT5G67060	63.235	68	25	0	18	85	114	181	5.70e-18	77.8
MsG0080048017.01.T01	AT5G67060	63.235	68	25	0	18	85	102	169	5.74e-18	77.8
MsG0080048017.01.T01	AT3G50330	63.235	68	25	0	18	85	111	178	9.29e-18	77.0
MsG0080048017.01.T01	AT1G27740	49.367	79	37	1	12	87	150	228	1.01e-17	77.4
MsG0080048017.01.T01	AT4G33880	56.667	60	26	0	28	87	268	327	1.04e-17	78.6
MsG0080048017.01.T01	AT5G09750	66.667	66	22	0	22	87	115	180	3.44e-17	75.5
MsG0080048017.01.T01	AT1G66470	48.235	85	42	2	3	85	170	254	7.24e-17	75.9
MsG0080048017.01.T01	AT2G14760	55.000	60	27	0	28	87	239	298	2.22e-16	74.7
MsG0080048017.01.T01	AT5G37800	60.784	51	20	0	35	85	210	260	4.02e-16	73.9
MsG0080048017.01.T01	AT4G30980	44.000	75	38	1	11	85	119	189	5.15e-14	68.2
MsG0080048017.01.T01	AT4G30980	44.000	75	38	1	11	85	167	237	6.65e-14	68.2
MsG0080048017.01.T01	AT2G24260	54.717	53	24	0	33	85	42	94	8.38e-14	67.0
MsG0080048017.01.T01	AT2G24260	54.717	53	24	0	33	85	145	197	2.46e-13	66.2
MsG0080048017.01.T01	AT2G14760	45.205	73	27	1	28	87	239	311	4.83e-13	65.5
MsG0080048017.01.T01	AT5G58010	43.478	69	35	1	17	85	94	158	3.95e-12	62.8

Find 24 sgRNAs with CRISPR-Local

Find 25 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACACTTTCTCTTATTCTTA+TGG	0.169939	contig171end:+18425	MsG0080048017.01.T01:CDS
ATTAACTTTGTTGATAATAT+TGG	0.286179	contig171end:+18689	MsG0080048017.01.T01:CDS
TCTCTAGCTGCAACACTTTG+TGG	0.303892	contig171end:-18521	None:intergenic
ACACTTTCTCTTATTCTTAT+GGG	0.318721	contig171end:+18426	MsG0080048017.01.T01:CDS
GATCCCTGGTGGAAGTAAAT+TGG	0.340425	contig171end:+18589	MsG0080048017.01.T01:CDS
AAGTTTCACTGAACTTCCTT+TGG	0.367586	contig171end:-18489	None:intergenic
AGTTTCTTAAGAAACAGATT+TGG	0.417653	contig171end:+18651	MsG0080048017.01.T01:CDS
TTTCATAGTGGCATCAAAGA+TGG	0.476710	contig171end:-18846	None:intergenic
ATTGGACACTGTTTCAATGT+TGG	0.489547	contig171end:+18607	MsG0080048017.01.T01:CDS
TATCATTTGAAGAATAATGT+TGG	0.491120	contig171end:-18732	None:intergenic
GAAGACATAGAATCAGTGAT+AGG	0.495589	contig171end:+18546	MsG0080048017.01.T01:CDS
GGACACTGTTTCAATGTTGG+AGG	0.517367	contig171end:+18610	MsG0080048017.01.T01:CDS
TGGGTCATGTTCAAAGAGAA+AGG	0.518124	contig171end:+18445	MsG0080048017.01.T01:CDS
GCATAGACTCAAAGAACTGA+AGG	0.532365	contig171end:-18763	None:intergenic
ATTTGAAGAATAATGTTGGA+AGG	0.535828	contig171end:-18728	None:intergenic
TTTGAAGAATAATGTTGGAA+GGG	0.552784	contig171end:-18727	None:intergenic
TTTCTTAGCTAGCACATGGA+TGG	0.556000	contig171end:+18401	None:intergenic
GTCTAATTAATATTTCATAG+TGG	0.574341	contig171end:-18858	None:intergenic
TGAAAACAGAACTGCTCTAG+TGG	0.577339	contig171end:-18803	None:intergenic
GTGTCCAATTTACTTCCACC+AGG	0.595621	contig171end:-18593	None:intergenic
ATTCTTCAGAGTATGATCCC+TGG	0.631016	contig171end:+18575	MsG0080048017.01.T01:CDS
CTTCAGAGTATGATCCCTGG+TGG	0.671280	contig171end:+18578	MsG0080048017.01.T01:CDS
AACTGCTCTAGTGGTAACTG+TGG	0.694715	contig171end:-18794	None:intergenic
TGTCCAATTTACTTCCACCA+GGG	0.712775	contig171end:-18592	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ATTAACTTTGTTGATAATAT+TGG	+	contig171end:18689-18708	MsG0080048017.01.T01:CDS	15.0%
!!	TATCATTTGAAGAATAATGT+TGG	-	contig171end:18735-18754	None:intergenic	20.0%
!	AACACTTTCTCTTATTCTTA+TGG	+	contig171end:18425-18444	MsG0080048017.01.T01:CDS	25.0%
!	ACACTTTCTCTTATTCTTAT+GGG	+	contig171end:18426-18445	MsG0080048017.01.T01:CDS	25.0%
!	AGTTTCTTAAGAAACAGATT+TGG	+	contig171end:18651-18670	MsG0080048017.01.T01:CDS	25.0%
!	ATTTGAAGAATAATGTTGGA+AGG	-	contig171end:18731-18750	None:intergenic	25.0%
!	TTTGAAGAATAATGTTGGAA+GGG	-	contig171end:18730-18749	None:intergenic	25.0%
	TCAAAGAGAAAGGAGAAAAA+AGG	+	contig171end:18455-18474	MsG0080048017.01.T01:CDS	30.0%
!	AAAGGTGTGAAAAAATCCAA+AGG	+	contig171end:18473-18492	MsG0080048017.01.T01:CDS	30.0%
	AAGTTTCACTGAACTTCCTT+TGG	-	contig171end:18492-18511	None:intergenic	35.0%
	ATTGGACACTGTTTCAATGT+TGG	+	contig171end:18607-18626	MsG0080048017.01.T01:CDS	35.0%
	GAAGACATAGAATCAGTGAT+AGG	+	contig171end:18546-18565	MsG0080048017.01.T01:CDS	35.0%
	TTTCATAGTGGCATCAAAGA+TGG	-	contig171end:18849-18868	None:intergenic	35.0%
	ATTCTTCAGAGTATGATCCC+TGG	+	contig171end:18575-18594	MsG0080048017.01.T01:CDS	40.0%
	GCATAGACTCAAAGAACTGA+AGG	-	contig171end:18766-18785	None:intergenic	40.0%
	TGAAAACAGAACTGCTCTAG+TGG	-	contig171end:18806-18825	None:intergenic	40.0%
	TGGGTCATGTTCAAAGAGAA+AGG	+	contig171end:18445-18464	MsG0080048017.01.T01:CDS	40.0%
	TGTCCAATTTACTTCCACCA+GGG	-	contig171end:18595-18614	None:intergenic	40.0%
!	CTAGAGCAGTTCTGTTTTCA+AGG	+	contig171end:18806-18825	MsG0080048017.01.T01:CDS	40.0%
	AACTGCTCTAGTGGTAACTG+TGG	-	contig171end:18797-18816	None:intergenic	45.0%
	GATCCCTGGTGGAAGTAAAT+TGG	+	contig171end:18589-18608	MsG0080048017.01.T01:CDS	45.0%
	GGACACTGTTTCAATGTTGG+AGG	+	contig171end:18610-18629	MsG0080048017.01.T01:CDS	45.0%
	GTGTCCAATTTACTTCCACC+AGG	-	contig171end:18596-18615	None:intergenic	45.0%
	TCTCTAGCTGCAACACTTTG+TGG	-	contig171end:18524-18543	None:intergenic	45.0%
	CTTCAGAGTATGATCCCTGG+TGG	+	contig171end:18578-18597	MsG0080048017.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
contig171end	gene	18416	18874	18416	ID=MsG0080048017.01;Name=MsG0080048017.01
contig171end	mRNA	18416	18874	18416	ID=MsG0080048017.01.T01;Parent=MsG0080048017.01;Name=MsG0080048017.01.T01;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|152
contig171end	exon	18416	18874	18416	ID=MsG0080048017.01.T01:exon:8809;Parent=MsG0080048017.01.T01
contig171end	CDS	18416	18874	18416	ID=MsG0080048017.01.T01:cds;Parent=MsG0080048017.01.T01

Gene Sequence

>MsG0080048017.01.T01

ATGGATGGTAACACTTTCTCTTATTCTTATGGGTCATGTTCAAAGAGAAAGGAGAAAAAAGGTGTGAAAAAATCCAAAGGAAGTTCAGTGAAACTTTCAACTGATCCACAAAGTGTTGCAGCTAGAGAAAGAAGACATAGAATCAGTGATAGGTTCAAGATTCTTCAGAGTATGATCCCTGGTGGAAGTAAATTGGACACTGTTTCAATGTTGGAGGAAGCTATTCATTATGTTAAGTTTCTTAAGAAACAGATTTGGCTTCATGAAACATTGATTAACTTTGTTGATAATATTGGTGAGTCACACATGTTCCCTTCCAACATTATTCTTCAAATGATAAAAATATTCCTTCAGTTCTTTGAGTCTATGCAGAATTTACCACAGTTACCACTAGAGCAGTTCTGTTTTCAAGGAGATGAAGATATCAAAACCATCTTTGATGCCACTATGAAATATTAA

Protein sequence

>MsG0080048017.01.T01

MDGNTFSYSYGSCSKRKEKKGVKKSKGSSVKLSTDPQSVAARERRHRISDRFKILQSMIPGGSKLDTVSMLEEAIHYVKFLKKQIWLHETLINFVDNIGESHMFPSNIILQMIKIFLQFFESMQNLPQLPLEQFCFQGDEDIKTIFDATMKY*