Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048130.01.T01 | RHN62886.1 | 82.353 | 51 | 9 | 0 | 2 | 52 | 48 | 98 | 4.80E-20 | 85.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048130.01.T01 | A0A396IB98 | 82.353 | 51 | 9 | 0 | 2 | 52 | 48 | 98 | 2.29e-20 | 85.9 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0080048130.01.T01 | TF | FAR1 |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048130.01.T01 | MTR_3g005220 | 66.000 | 50 | 14 | 1 | 1 | 50 | 79 | 125 | 1.88e-17 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 11 sgRNAs with CRISPR-Local
Find 14 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGAACAAATGGATTGATAAT+TGG | 0.321466 | contig215end:-5532 | MsG0080048130.01.T01:CDS |
| ATAACTAAATCAAGAACAAA+TGG | 0.450621 | contig215end:-5544 | MsG0080048130.01.T01:CDS |
| AAAGGTTGTAGCTTTCGCTT+GGG | 0.477046 | contig215end:-5571 | MsG0080048130.01.T01:CDS |
| CAAAGGTTGTAGCTTTCGCT+TGG | 0.479905 | contig215end:-5572 | MsG0080048130.01.T01:CDS |
| AAATGGATTGATAATTGGTC+AGG | 0.480078 | contig215end:-5527 | MsG0080048130.01.T01:CDS |
| TCAGGAGATTCTTTGTTCAA+AGG | 0.531562 | contig215end:-5509 | MsG0080048130.01.T01:CDS |
| AGATTCTTTGTTCAAAGGAG+GGG | 0.531979 | contig215end:-5504 | MsG0080048130.01.T01:CDS |
| GAGATTCTTTGTTCAAAGGA+GGG | 0.547884 | contig215end:-5505 | MsG0080048130.01.T01:CDS |
| GGAGATTCTTTGTTCAAAGG+AGG | 0.550163 | contig215end:-5506 | MsG0080048130.01.T01:CDS |
| ATTACTATACGGGATATGAA+AGG | 0.604315 | contig215end:-5597 | MsG0080048130.01.T01:CDS |
| ACGGGATATGAAAGGAACAA+AGG | 0.634481 | contig215end:-5589 | MsG0080048130.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATGCAAAAAATTACTATA+CGG | - | contig215end:5503-5522 | MsG0080048130.01.T01:CDS | 20.0% |
| !! | ATAACTAAATCAAGAACAAA+TGG | - | contig215end:5567-5586 | MsG0080048130.01.T01:CDS | 20.0% |
| ! | GATGCAAAAAATTACTATAC+GGG | - | contig215end:5504-5523 | MsG0080048130.01.T01:CDS | 25.0% |
| !!! | AGAACAAATGGATTGATAAT+TGG | - | contig215end:5579-5598 | MsG0080048130.01.T01:CDS | 25.0% |
| ATTACTATACGGGATATGAA+AGG | - | contig215end:5514-5533 | MsG0080048130.01.T01:CDS | 30.0% | |
| !! | AAATGGATTGATAATTGGTC+AGG | - | contig215end:5584-5603 | MsG0080048130.01.T01:CDS | 30.0% |
| !!! | GTAATTTTTTGCATCTTCGA+AGG | + | contig215end:5499-5518 | MsG0080048130.01.T01:intergenic | 30.0% |
| AGATTCTTTGTTCAAAGGAG+GGG | - | contig215end:5607-5626 | MsG0080048130.01.T01:CDS | 35.0% | |
| GAGATTCTTTGTTCAAAGGA+GGG | - | contig215end:5606-5625 | MsG0080048130.01.T01:CDS | 35.0% | |
| TCAGGAGATTCTTTGTTCAA+AGG | - | contig215end:5602-5621 | MsG0080048130.01.T01:CDS | 35.0% | |
| AAAGGTTGTAGCTTTCGCTT+GGG | - | contig215end:5540-5559 | MsG0080048130.01.T01:CDS | 40.0% | |
| ACGGGATATGAAAGGAACAA+AGG | - | contig215end:5522-5541 | MsG0080048130.01.T01:CDS | 40.0% | |
| GGAGATTCTTTGTTCAAAGG+AGG | - | contig215end:5605-5624 | MsG0080048130.01.T01:CDS | 40.0% | |
| CAAAGGTTGTAGCTTTCGCT+TGG | - | contig215end:5539-5558 | MsG0080048130.01.T01:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig215end | gene | 5483 | 5650 | 5483 | ID=MsG0080048130.01;Name=MsG0080048130.01 |
| contig215end | mRNA | 5483 | 5650 | 5483 | ID=MsG0080048130.01.T01;Parent=MsG0080048130.01;Name=MsG0080048130.01.T01;_AED=0.52;_eAED=0.52;_QI=0|-1|0|1|-1|0|1|0|55 |
| contig215end | exon | 5483 | 5650 | 5483 | ID=MsG0080048130.01.T01:exon:38823;Parent=MsG0080048130.01.T01 |
| contig215end | CDS | 5483 | 5650 | 5483 | ID=MsG0080048130.01.T01:cds;Parent=MsG0080048130.01.T01 |
| Gene Sequence |
| Protein sequence |
