AlfalfaGEDB | MsG0080048436.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048436.01

KEGG: -
GO: GO:0003677 GO:0046983
PFAM: PF00319

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048436.01.T01	XP_013469972.1	84.906	53	8	0	1	53	15	67	1.32E-23	97.4

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048436.01.T01	Q9FKK2	77.358	53	12	0	1	53	13	65	3.47E-23	91.3

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048436.01.T01	A0A072W146	84.906	53	8	0	1	53	15	67	6.29e-24	97.4

TFs/TRs:

Gene ID	Type	Classification
MsG0080048436.01.T01	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048436.01.T01	MTR_1g106070	84.906	53	8	0	1	53	15	67	1.59e-27	97.4
MsG0080048436.01.T01	MTR_1g105910	83.019	53	9	0	1	53	15	67	2.25e-26	95.9
MsG0080048436.01.T01	MTR_3g052870	83.019	53	9	0	1	53	15	67	3.38e-26	95.1
MsG0080048436.01.T01	MTR_8g043650	80.000	55	11	0	1	55	15	69	3.49e-26	95.5
MsG0080048436.01.T01	MTR_1g105920	83.019	53	9	0	1	53	15	67	6.43e-26	94.7
MsG0080048436.01.T01	MTR_1g012570	79.245	53	11	0	1	53	15	67	3.53e-25	92.8
MsG0080048436.01.T01	MTR_3g052920	76.364	55	13	0	1	55	15	69	3.14e-24	90.1
MsG0080048436.01.T01	MTR_5g045560	77.358	53	12	0	1	53	15	67	4.03e-24	90.1
MsG0080048436.01.T01	MTR_1g108510	78.182	55	12	0	1	55	15	69	4.57e-24	89.7
MsG0080048436.01.T01	MTR_3g030770	78.182	55	12	0	1	55	15	69	6.11e-24	89.4
MsG0080048436.01.T01	MTR_2g093190	78.182	55	12	0	1	55	15	69	1.08e-23	88.6
MsG0080048436.01.T01	MTR_2g085250	73.585	53	14	0	1	53	15	67	1.32e-23	88.6
MsG0080048436.01.T01	MTR_2g085280	73.585	53	14	0	1	53	15	67	1.33e-23	88.6
MsG0080048436.01.T01	MTR_2g030740	77.358	53	12	0	1	53	15	67	1.58e-23	88.6
MsG0080048436.01.T01	MTR_1g105905	80.000	50	10	0	4	53	1	50	1.97e-23	87.8
MsG0080048436.01.T01	MTR_1g077360	75.472	53	13	0	1	53	15	67	6.19e-23	87.0
MsG0080048436.01.T01	MTR_1g075600	75.472	53	13	0	1	53	15	67	1.16e-22	86.3
MsG0080048436.01.T01	MTR_5g053390	73.585	53	14	0	1	53	15	67	1.20e-22	86.3
MsG0080048436.01.T01	MTR_5g055100	71.698	53	15	0	1	53	15	67	1.82e-22	85.9
MsG0080048436.01.T01	MTR_0121s0100	70.909	55	16	0	1	55	15	69	2.55e-22	82.8
MsG0080048436.01.T01	MTR_1g108580	72.727	55	15	0	1	55	15	69	5.78e-22	84.3
MsG0080048436.01.T01	MTR_3g030780	69.811	53	16	0	1	53	15	67	1.44e-21	80.9
MsG0080048436.01.T01	MTR_3g067875	70.000	50	15	0	4	53	1	50	4.13e-21	77.8
MsG0080048436.01.T01	MTR_0121s0080	69.811	53	16	0	1	53	15	67	2.02e-20	80.5
MsG0080048436.01.T01	MTR_3g080940	64.151	53	19	0	1	53	15	67	1.27e-19	77.8
MsG0080048436.01.T01	MTR_1g108500	69.565	46	14	0	8	53	110	155	2.48e-17	73.2
MsG0080048436.01.T01	MTR_1g075570	74.419	43	11	0	4	46	1	43	4.97e-17	70.1
MsG0080048436.01.T01	MTR_3g067870	59.615	52	21	0	2	53	83	134	6.78e-16	67.0
MsG0080048436.01.T01	MTR_7g028448	65.217	46	16	0	8	53	25	70	1.38e-15	66.6
MsG0080048436.01.T01	MTR_4g036915	65.217	46	16	0	8	53	25	70	1.38e-15	66.6
MsG0080048436.01.T01	MTR_7g062350	60.377	53	20	1	2	53	27	79	2.11e-15	67.4
MsG0080048436.01.T01	MTR_8g079502	67.442	43	14	0	11	53	18	60	2.14e-15	66.2
MsG0080048436.01.T01	MTR_1g047550	67.442	43	14	0	11	53	18	60	7.21e-15	64.7
MsG0080048436.01.T01	MTR_8g086290	50.877	57	28	0	1	57	18	74	9.49e-15	64.3
MsG0080048436.01.T01	MTR_1g054265	50.943	53	26	0	1	53	8	60	2.18e-14	63.2
MsG0080048436.01.T01	MTR_8g051580	62.791	43	16	0	11	53	18	60	2.65e-14	63.5
MsG0080048436.01.T01	MTR_2g105290	50.943	53	26	0	1	53	18	70	2.77e-14	63.2
MsG0080048436.01.T01	MTR_4g127140	58.696	46	19	0	8	53	25	70	4.04e-14	63.2
MsG0080048436.01.T01	MTR_4g093970	56.140	57	25	0	1	57	34	90	4.79e-14	63.5
MsG0080048436.01.T01	MTR_4g131030	62.791	43	16	0	11	53	18	60	6.44e-14	62.8
MsG0080048436.01.T01	MTR_3g067910	55.556	54	21	1	7	57	2	55	1.22e-13	60.1
MsG0080048436.01.T01	MTR_2g461710	53.704	54	24	1	1	53	8	61	2.09e-13	58.2
MsG0080048436.01.T01	MTR_1g063160	49.057	53	27	0	1	53	8	60	2.20e-13	60.8
MsG0080048436.01.T01	MTR_8g046350	58.140	43	18	0	11	53	18	60	3.40e-13	60.1
MsG0080048436.01.T01	MTR_7g075870	46.552	58	31	0	1	58	8	65	4.89e-13	60.5
MsG0080048436.01.T01	MTR_8g033220	48.276	58	30	0	1	58	8	65	6.08e-13	60.5
MsG0080048436.01.T01	MTR_7g075870	50.000	52	26	0	1	52	8	59	8.15e-13	60.1
MsG0080048436.01.T01	MTR_8g033220	48.276	58	30	0	1	58	8	65	8.31e-13	60.1
MsG0080048436.01.T01	MTR_4g102530	53.846	52	24	0	1	52	8	59	2.09e-12	58.9
MsG0080048436.01.T01	MTR_4g094632	52.000	50	24	0	4	53	11	60	2.22e-12	57.8
MsG0080048436.01.T01	MTR_5g032520	55.102	49	22	0	1	49	8	56	2.25e-12	58.9
MsG0080048436.01.T01	MTR_6g464720	48.214	56	29	0	1	56	8	63	2.45e-12	58.9
MsG0080048436.01.T01	MTR_4g036050	44.828	58	32	0	1	58	8	65	2.51e-12	58.2
MsG0080048436.01.T01	MTR_4g036050	45.614	57	31	0	1	57	8	64	3.21e-12	58.2
MsG0080048436.01.T01	MTR_4g036050	45.614	57	31	0	1	57	8	64	3.25e-12	58.2
MsG0080048436.01.T01	MTR_4g036050	45.614	57	31	0	1	57	8	64	3.81e-12	58.2
MsG0080048436.01.T01	MTR_1g101970	50.909	55	27	0	1	55	8	62	4.02e-12	57.8
MsG0080048436.01.T01	MTR_5g032150	56.250	48	21	0	1	48	8	55	7.37e-12	57.8
MsG0080048436.01.T01	MTR_4g084740	49.020	51	26	0	1	51	8	58	7.52e-12	58.2
MsG0080048436.01.T01	MTR_8g066260	51.923	52	25	0	1	52	8	59	9.61e-12	57.4
MsG0080048436.01.T01	MTR_0003s0590	48.077	52	27	0	1	52	8	59	1.24e-11	57.4
MsG0080048436.01.T01	MTR_5g066960	41.509	53	31	0	1	53	8	60	2.06e-11	53.1
MsG0080048436.01.T01	MTR_3g102570	56.522	46	20	0	1	46	8	53	2.21e-11	57.0
MsG0080048436.01.T01	MTR_4g109830	48.077	52	27	0	1	52	8	59	2.85e-11	56.2
MsG0080048436.01.T01	MTR_3g452380	51.923	52	25	0	1	52	23	74	3.05e-11	56.2
MsG0080048436.01.T01	MTR_8g087860	50.000	52	26	0	1	52	24	75	4.27e-11	55.8
MsG0080048436.01.T01	MTR_5g066180	50.000	52	26	0	1	52	8	59	4.54e-11	55.5
MsG0080048436.01.T01	MTR_2g017865	50.000	52	26	0	1	52	23	74	5.14e-11	55.5
MsG0080048436.01.T01	MTR_5g046790	46.154	52	28	0	1	52	8	59	5.74e-11	55.1
MsG0080048436.01.T01	MTR_6g015975	44.068	59	32	1	1	58	8	66	6.14e-11	55.5
MsG0080048436.01.T01	MTR_1g053070	48.077	52	27	0	1	52	8	59	6.62e-11	55.1
MsG0080048436.01.T01	MTR_4g094638	49.057	53	27	0	1	53	8	60	6.83e-11	54.3

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048436.01.T01	AT5G60440	77.358	53	12	1	53	13	65	3.54e-24	91.3
MsG0080048436.01.T01	AT4G36590	69.811	53	16	1	53	13	65	1.28e-20	81.3
MsG0080048436.01.T01	AT2G24840	62.264	53	20	1	53	69	121	3.09e-19	77.8
MsG0080048436.01.T01	AT1G01530	64.706	51	18	3	53	15	65	1.04e-18	76.3
MsG0080048436.01.T01	AT1G65360	60.784	51	20	3	53	15	65	1.16e-17	73.2
MsG0080048436.01.T01	AT2G34440	64.444	45	16	11	55	18	62	5.45e-15	65.1
MsG0080048436.01.T01	AT3G66656	56.604	53	23	1	53	8	60	1.20e-14	64.3
MsG0080048436.01.T01	AT1G71692	51.923	52	25	1	52	8	59	7.19e-14	62.8
MsG0080048436.01.T01	AT4G22950	49.091	55	28	1	55	8	62	9.33e-14	62.0
MsG0080048436.01.T01	AT2G45660	50.000	52	26	1	52	8	59	2.15e-13	60.5
MsG0080048436.01.T01	AT4G11880	48.214	56	29	1	56	8	63	5.49e-13	60.1
MsG0080048436.01.T01	AT4G11880	48.214	56	29	1	56	8	63	5.49e-13	60.1
MsG0080048436.01.T01	AT4G22950	51.923	52	25	1	52	8	59	7.58e-13	60.1
MsG0080048436.01.T01	AT4G22950	51.923	52	25	1	52	8	59	7.58e-13	60.1
MsG0080048436.01.T01	AT2G22540	54.000	50	23	1	50	8	57	2.56e-12	58.9
MsG0080048436.01.T01	AT5G60910	50.943	53	26	1	53	8	60	2.83e-12	58.9
MsG0080048436.01.T01	AT5G51860	53.846	52	24	1	52	8	59	3.52e-12	58.2
MsG0080048436.01.T01	AT2G22540	51.923	52	25	1	52	8	59	3.61e-12	58.5
MsG0080048436.01.T01	AT2G22540	51.923	52	25	1	52	8	59	3.61e-12	58.5
MsG0080048436.01.T01	AT4G11880	50.000	52	26	1	52	8	59	4.63e-12	58.2
MsG0080048436.01.T01	AT4G11880	50.000	52	26	1	52	8	59	4.63e-12	58.2
MsG0080048436.01.T01	AT5G51860	53.846	52	24	1	52	8	59	4.67e-12	58.2
MsG0080048436.01.T01	AT3G30260	51.020	49	24	1	49	8	56	5.82e-12	58.2
MsG0080048436.01.T01	AT5G51870	60.870	46	18	1	46	8	53	5.84e-12	57.0
MsG0080048436.01.T01	AT2G45660	50.000	52	26	1	52	8	59	6.45e-12	57.8
MsG0080048436.01.T01	AT4G24540	51.923	52	25	1	52	8	59	6.79e-12	57.8
MsG0080048436.01.T01	AT5G62165	45.455	55	30	1	55	8	62	8.66e-12	55.5
MsG0080048436.01.T01	AT5G13790	46.552	58	31	1	58	8	65	9.18e-12	57.8
MsG0080048436.01.T01	AT5G13790	46.552	58	31	1	58	8	65	9.23e-12	57.8
MsG0080048436.01.T01	AT5G51870	60.870	46	18	1	46	31	76	1.21e-11	56.6
MsG0080048436.01.T01	AT5G51870	60.870	46	18	1	46	8	53	1.25e-11	56.6
MsG0080048436.01.T01	AT3G57230	41.818	55	32	1	55	8	62	1.27e-11	55.8
MsG0080048436.01.T01	AT5G62165	47.170	53	28	1	53	8	60	1.29e-11	55.8
MsG0080048436.01.T01	AT5G62165	47.170	53	28	1	53	8	60	1.29e-11	55.8
MsG0080048436.01.T01	AT5G62165	47.170	53	28	1	53	8	60	1.29e-11	55.8
MsG0080048436.01.T01	AT1G22130	54.348	46	21	1	46	8	53	1.33e-11	57.4
MsG0080048436.01.T01	AT1G77980	54.348	46	21	1	46	8	53	1.45e-11	57.4
MsG0080048436.01.T01	AT5G62165	47.170	53	28	1	53	8	60	1.58e-11	55.8
MsG0080048436.01.T01	AT5G62165	47.170	53	28	1	53	8	60	1.58e-11	55.8
MsG0080048436.01.T01	AT5G62165	47.170	53	28	1	53	8	60	1.58e-11	55.8
MsG0080048436.01.T01	AT5G62165	47.170	53	28	1	53	8	60	1.58e-11	55.8
MsG0080048436.01.T01	AT5G62165	47.170	53	28	1	53	8	60	1.58e-11	55.8
MsG0080048436.01.T01	AT5G51870	60.870	46	18	1	46	8	53	1.74e-11	56.6
MsG0080048436.01.T01	AT5G51870	60.870	46	18	1	46	18	63	1.97e-11	56.6
MsG0080048436.01.T01	AT1G72350	52.083	48	23	1	48	49	96	2.46e-11	56.2
MsG0080048436.01.T01	AT1G69120	51.923	52	25	1	52	8	59	3.31e-11	56.2
MsG0080048436.01.T01	AT2G45650	48.077	52	27	1	52	8	59	3.76e-11	55.8
MsG0080048436.01.T01	AT1G69120	51.923	52	25	1	52	8	59	3.92e-11	55.8
MsG0080048436.01.T01	AT3G58780	50.000	52	26	1	52	23	74	5.08e-11	55.8
MsG0080048436.01.T01	AT3G54340	43.396	53	30	1	53	8	60	6.99e-11	55.1
MsG0080048436.01.T01	AT3G58780	48.214	56	29	1	56	23	78	9.85e-11	55.1

Find 16 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCTCTCGTTGTTTACTCACC+TGG	0.370542	contig319end:-1572	MsG0080048436.01.T01:CDS
CTCTCGTTGTTTACTCACCT+GGG	0.441719	contig319end:-1571	MsG0080048436.01.T01:CDS
ACAGAGGGTGCAAAGCTCAC+TGG	0.525840	contig319end:+1607	None:intergenic
ATCTATGACTGTATCAACAC+TGG	0.540768	contig319end:+1524	None:intergenic
TTGTTTACTCACCTGGGAGA+AGG	0.551913	contig319end:-1565	MsG0080048436.01.T01:CDS
CTTTCTCGAAGCGCCGTAGT+GGG	0.553366	contig319end:-1643	MsG0080048436.01.T01:CDS
ACTTTCTCGAAGCGCCGTAG+TGG	0.571963	contig319end:-1644	MsG0080048436.01.T01:CDS
AGTGAGCTTTGCACCCTCTG+TGG	0.587031	contig319end:-1605	MsG0080048436.01.T01:CDS
AGCAACGTCTGCACCACAGA+GGG	0.589941	contig319end:+1592	None:intergenic
TCGAGAAAGTCACTTGCAAG+TGG	0.591713	contig319end:+1657	None:intergenic
TCTATGACTGTATCAACACT+GGG	0.592150	contig319end:+1525	None:intergenic
AAATGAAAATACCTTCTCCC+AGG	0.608320	contig319end:+1554	None:intergenic
CTTTCTTGAAGAGCCCACTA+CGG	0.610979	contig319end:+1630	None:intergenic
TGACTGTATCAACACTGGGG+TGG	0.658147	contig319end:+1529	None:intergenic
GAGCAACGTCTGCACCACAG+AGG	0.729838	contig319end:+1591	None:intergenic
CTATGACTGTATCAACACTG+GGG	0.806256	contig319end:+1526	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	AAATGAAAATACCTTCTCCC+AGG	+	contig319end:1655-1674	None:intergenic	35.0%
!!!	TGGGAGAAGGTATTTTCATT+TGG	-	contig319end:1654-1673	MsG0080048436.01.T01:CDS	35.0%
	CTATGACTGTATCAACACTG+GGG	+	contig319end:1683-1702	None:intergenic	40.0%
	CTCTCGTTGTTTACTCACCT+GGG	-	contig319end:1635-1654	MsG0080048436.01.T01:CDS	45.0%
	CTTTCTTGAAGAGCCCACTA+CGG	+	contig319end:1579-1598	None:intergenic	45.0%
	TCGAGAAAGTCACTTGCAAG+TGG	+	contig319end:1552-1571	None:intergenic	45.0%
	TTGTTTACTCACCTGGGAGA+AGG	-	contig319end:1641-1660	MsG0080048436.01.T01:CDS	45.0%
	GCTCTCGTTGTTTACTCACC+TGG	-	contig319end:1634-1653	MsG0080048436.01.T01:CDS	50.0%
	TGACTGTATCAACACTGGGG+TGG	+	contig319end:1680-1699	None:intergenic	50.0%
	AGCAACGTCTGCACCACAGA+GGG	+	contig319end:1617-1636	None:intergenic	55.0%
	AGTGAGCTTTGCACCCTCTG+TGG	-	contig319end:1601-1620	MsG0080048436.01.T01:CDS	55.0%
!	ACAGAGGGTGCAAAGCTCAC+TGG	+	contig319end:1602-1621	None:intergenic	55.0%
!!	ACTTTCTCGAAGCGCCGTAG+TGG	-	contig319end:1562-1581	MsG0080048436.01.T01:CDS	55.0%
!!	CTTTCTCGAAGCGCCGTAGT+GGG	-	contig319end:1563-1582	MsG0080048436.01.T01:CDS	55.0%
	GAGCAACGTCTGCACCACAG+AGG	+	contig319end:1618-1637	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
contig319end	gene	1526	1702	1526	ID=MsG0080048436.01;Name=MsG0080048436.01
contig319end	mRNA	1526	1702	1526	ID=MsG0080048436.01.T01;Parent=MsG0080048436.01;Name=MsG0080048436.01.T01;_AED=0.37;_eAED=0.37;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|58
contig319end	exon	1526	1702	1526	ID=MsG0080048436.01.T01:exon:1518;Parent=MsG0080048436.01.T01
contig319end	CDS	1526	1702	1526	ID=MsG0080048436.01.T01:cds;Parent=MsG0080048436.01.T01

Gene Sequence	>MsG0080048436.01.T01 ATGAAAAAAATGAGCAACGAGAGCCACTTGCAAGTGACTTTCTCGAAGCGCCGTAGTGGGCTCTTCAAGAAAGCCAGTGAGCTTTGCACCCTCTGTGGTGCAGACGTTGCTCTCGTTGTTTACTCACCTGGGAGAAGGTATTTTCATTTGGCCACCCCAGTGTTGATACAGTCATAG
Protein sequence	>MsG0080048436.01.T01 MKKMSNESHLQVTFSKRRSGLFKKASELCTLCGADVALVVYSPGRRYFHLATPVLIQS*