AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000238.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000238.01.T01 MTR_1g019820 79.253 241 22 4 98 338 5 217 1.19e-123 354
MsG0180000238.01.T01 MTR_1g045470 52.199 341 123 8 1 331 1 311 9.34e-105 310
MsG0180000238.01.T01 MTR_7g116460 69.524 210 49 2 1 203 1 202 2.14e-95 282
MsG0180000238.01.T01 MTR_1g053575 44.077 363 117 10 1 331 1 309 3.29e-86 263
MsG0180000238.01.T01 MTR_7g100990 35.032 157 87 5 11 159 17 166 2.37e-18 84.7
MsG0180000238.01.T01 MTR_7g005280 32.099 162 98 4 11 170 21 172 2.30e-17 80.9
MsG0180000238.01.T01 MTR_2g093810 29.741 232 123 9 11 209 20 244 7.40e-14 71.6
MsG0180000238.01.T01 MTR_4g089135 34.109 129 76 4 11 137 21 142 3.74e-13 69.7
MsG0180000238.01.T01 MTR_8g094580 30.603 232 123 11 11 215 13 233 1.15e-12 67.8
MsG0180000238.01.T01 MTR_2g080010 26.872 227 124 10 11 219 21 223 1.79e-12 67.8
MsG0180000238.01.T01 MTR_5g041940 28.431 204 123 8 11 204 9 199 5.64e-12 65.5
MsG0180000238.01.T01 MTR_2157s0010 29.114 158 91 6 11 154 14 164 6.18e-12 65.5
MsG0180000238.01.T01 MTR_4g081870 30.968 155 90 6 11 157 15 160 1.05e-11 64.7
MsG0180000238.01.T01 MTR_8g076110 28.877 187 115 8 10 193 11 182 1.23e-11 65.1
MsG0180000238.01.T01 MTR_5g040420 27.907 215 126 9 11 219 20 211 1.26e-11 65.5
MsG0180000238.01.T01 MTR_3g096140 31.250 160 97 8 11 165 13 164 1.46e-11 64.7
MsG0180000238.01.T01 MTR_3g096140 31.250 160 97 8 11 165 13 164 2.15e-11 64.7
MsG0180000238.01.T01 MTR_1g096430 29.677 155 99 6 11 163 13 159 2.60e-11 64.3
MsG0180000238.01.T01 MTR_8g093580 31.481 162 94 9 11 165 12 163 5.67e-11 63.9
MsG0180000238.01.T01 MTR_3g109340 27.517 149 99 3 11 157 28 169 8.10e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000238.01.T01 AT5G13180 34.014 147 84 5 11 154 20 156 1.69e-14 72.8
MsG0180000238.01.T01 AT3G15510 28.723 188 105 7 11 182 23 197 3.84e-14 73.2
MsG0180000238.01.T01 AT1G77450 31.788 151 86 6 11 154 16 156 1.27e-12 67.4
MsG0180000238.01.T01 AT5G08790 34.194 155 79 8 11 154 13 155 7.00e-12 65.5
MsG0180000238.01.T01 AT3G10480 33.962 159 82 9 11 158 33 179 1.19e-11 65.9
MsG0180000238.01.T01 AT3G10480 33.766 154 88 8 11 158 33 178 2.47e-11 65.1
MsG0180000238.01.T01 AT3G61910 25.962 208 122 9 11 192 17 218 2.86e-11 64.3
MsG0180000238.01.T01 AT3G04070 31.677 161 89 8 11 157 16 169 2.91e-11 64.3
MsG0180000238.01.T01 AT3G15170 31.333 150 90 5 11 154 26 168 3.01e-11 63.9
MsG0180000238.01.T01 AT4G27410 32.258 155 84 6 11 157 20 161 4.50e-11 63.2
MsG0180000238.01.T01 AT4G36160 30.864 162 100 7 11 167 20 174 4.55e-11 63.9
MsG0180000238.01.T01 AT4G36160 30.864 162 100 7 11 167 16 170 4.61e-11 63.9
MsG0180000238.01.T01 AT4G36160 30.864 162 100 7 11 167 16 170 4.61e-11 63.9
MsG0180000238.01.T01 AT1G12260 30.719 153 95 7 11 159 13 158 5.46e-11 63.5
MsG0180000238.01.T01 AT1G32770 32.099 162 89 7 11 157 22 177 5.81e-11 63.5
MsG0180000238.01.T01 AT1G12260 30.719 153 95 7 11 159 13 158 6.19e-11 63.5

Find 67 sgRNAs with CRISPR-Local

Find 186 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAAGTTGATTTATTGAATT+TGG 0.033936 1:+3265121 None:intergenic
CCACCATTTGATTGACATTT+TGG 0.106202 1:+3266107 None:intergenic
ATAGCAATGTTTGCCAAAAT+TGG 0.116243 1:+3264907 None:intergenic
ATTGAAATGATTCCCAATTT+TGG 0.141545 1:-3264920 MsG0180000238.01.T01:CDS
GTTCTTGAATATGATTTATT+TGG 0.182094 1:-3268777 MsG0180000238.01.T01:CDS
CAAAGAATCCTTGGGAGATT+TGG 0.196255 1:-3268749 MsG0180000238.01.T01:CDS
AGTGGGAGATTTCTTCTTTA+AGG 0.222616 1:+3268671 None:intergenic
CCAGAAAAGACCGTGGTTCT+TGG 0.260366 1:-3266053 MsG0180000238.01.T01:CDS
AAAGAATCCTTGGGAGATTT+GGG 0.318962 1:-3268748 MsG0180000238.01.T01:CDS
CAAGGAAAGACAGTTGTTTC+CGG 0.324971 1:-3268103 MsG0180000238.01.T01:CDS
TTGATTCGTACAATTGGAAT+TGG 0.348604 1:-3268639 MsG0180000238.01.T01:CDS
TTCAAGAACATTGATATAGT+TGG 0.354618 1:+3268791 None:intergenic
CAAGTTGATTTATTGAATTT+GGG 0.363697 1:+3265122 None:intergenic
TTTGCAAGGGAACTCTTAGA+AGG 0.370091 1:-3264974 MsG0180000238.01.T01:CDS
TCATCAAGTGAGAATTTGAT+TGG 0.398760 1:+3268146 None:intergenic
GAGAACAATGATGTTTGGTT+GGG 0.407569 1:-3265157 MsG0180000238.01.T01:CDS
AAACGCTTGATTCGTACAAT+TGG 0.407900 1:-3268645 MsG0180000238.01.T01:CDS
TAGCAATGTTTGCCAAAATT+GGG 0.411997 1:+3264908 None:intergenic
TTGGTATCACAAAGAATCCT+TGG 0.412757 1:-3268758 MsG0180000238.01.T01:CDS
ATCTTTGATTAACAATGCTT+CGG 0.415664 1:-3268472 MsG0180000238.01.T01:intron
TGCAGGAGAACAATGATGTT+TGG 0.432945 1:-3265162 MsG0180000238.01.T01:intron
TCAAGAACATTGATATAGTT+GGG 0.434618 1:+3268792 None:intergenic
TCAATAAATCAACTTGATAG+TGG 0.436487 1:-3265115 MsG0180000238.01.T01:CDS
AGTATGGTAACTTACAGATA+TGG 0.443956 1:+3268066 None:intergenic
GAACCAAAATGTCAATCAAA+TGG 0.458333 1:-3266110 MsG0180000238.01.T01:CDS
ACAAATAATACTTACAATGT+TGG 0.484031 1:+3266016 None:intergenic
TCTGCATAGAACATAATTGC+TGG 0.484592 1:+3268222 None:intergenic
ATGAAGATGGTGATATCTCT+TGG 0.494027 1:-3265006 MsG0180000238.01.T01:CDS
ACGAATCAAGCGTTTACTAG+TGG 0.496910 1:+3268653 None:intergenic
GGAGAAAGGTGGGAAATACT+TGG 0.497376 1:-3266076 MsG0180000238.01.T01:CDS
AATCAAATGGTGGACACAAA+AGG 0.500915 1:-3266097 MsG0180000238.01.T01:CDS
TGGTATCACAAAGAATCCTT+GGG 0.502644 1:-3268757 MsG0180000238.01.T01:CDS
AATTGTAAACTTTATTGTAG+AGG 0.517878 1:+3268823 None:intergenic
AAGAGTAACACTTCTGAAGA+TGG 0.525582 1:-3268528 MsG0180000238.01.T01:CDS
AGTATAGTTGACAAAGAGAC+TGG 0.529604 1:-3268588 MsG0180000238.01.T01:CDS
GGAGAACAATGATGTTTGGT+TGG 0.533458 1:-3265158 MsG0180000238.01.T01:CDS
ATTATAAACAAAGATGAAGA+TGG 0.538976 1:-3265019 MsG0180000238.01.T01:CDS
TCTCACTTGATGAATAGCAT+TGG 0.540753 1:-3268136 MsG0180000238.01.T01:CDS
TTGCAAGGGAACTCTTAGAA+GGG 0.552996 1:-3264973 MsG0180000238.01.T01:CDS
AACTCTTCCCAAATCTCCCA+AGG 0.556547 1:+3268741 None:intergenic
AGAACAATGATGTTTGGTTG+GGG 0.562858 1:-3265156 MsG0180000238.01.T01:CDS
CGAATCAAGCGTTTACTAGT+GGG 0.567217 1:+3268654 None:intergenic
GAACTCTTAGAAGGGAATGA+AGG 0.570693 1:-3264965 MsG0180000238.01.T01:CDS
CGAAGAAGTTCACCATCTGA+AGG 0.571056 1:+3268852 None:intergenic
TTTAGGACATCATTGTTTCG+AGG 0.573378 1:-3266148 MsG0180000238.01.T01:intron
TGGAAATATCAAGATTGACT+TGG 0.581332 1:-3265095 MsG0180000238.01.T01:CDS
GACAGTTGTTTCCGGACCTG+CGG 0.583589 1:-3268095 MsG0180000238.01.T01:CDS
AAAGAGACTGGCCAAACCCT+CGG 0.584271 1:-3268576 MsG0180000238.01.T01:CDS
ACACTTCTGAAGATGGTGGA+TGG 0.587791 1:-3268521 MsG0180000238.01.T01:CDS
ACATTGATATAGTTGGGCAC+TGG 0.596165 1:+3268798 None:intergenic
TGGTGGACACAAAAGGAGAA+AGG 0.597243 1:-3266090 MsG0180000238.01.T01:CDS
CCAAAATGTCAATCAAATGG+TGG 0.617859 1:-3266107 MsG0180000238.01.T01:CDS
TGTTAATCAAAGATATGTCA+AGG 0.619881 1:+3268481 None:intergenic
GGACACAAAAGGAGAAAGGT+GGG 0.621854 1:-3266086 MsG0180000238.01.T01:CDS
AGGAAGCTTAATCGAAGAGG+AGG 0.624584 1:-3264945 MsG0180000238.01.T01:CDS
GTACAATTGGAATTGGTACG+TGG 0.632001 1:-3268632 MsG0180000238.01.T01:CDS
AGTAACACTTCTGAAGATGG+TGG 0.632536 1:-3268525 MsG0180000238.01.T01:CDS
TGGACACAAAAGGAGAAAGG+TGG 0.644978 1:-3266087 MsG0180000238.01.T01:CDS
AAGTGTTACTCTTCTCAAAG+CGG 0.648652 1:+3268538 None:intergenic
AGCATTGGACACGATAGGCA+AGG 0.655461 1:-3268121 MsG0180000238.01.T01:CDS
TGAAGGAAGCTTAATCGAAG+AGG 0.655839 1:-3264948 MsG0180000238.01.T01:CDS
TACAATTGGAATTGGTACGT+GGG 0.659468 1:-3268631 MsG0180000238.01.T01:CDS
ACGTGGGAAGCTGAAGACAC+CGG 0.664319 1:-3268615 MsG0180000238.01.T01:CDS
TGAATAGCATTGGACACGAT+AGG 0.665350 1:-3268126 MsG0180000238.01.T01:CDS
AGCTTTAAGTCCAAGAACCA+CGG 0.675233 1:+3266043 None:intergenic
TAAAAGTGCCAATACAACAG+AGG 0.692386 1:-3265071 MsG0180000238.01.T01:CDS
TACTTGGCCAGAAAAGACCG+TGG 0.736153 1:-3266060 MsG0180000238.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACTAATTAATACTATTAATT+TGG + Chr1:3267027-3267046 None:intergenic 10.0%
!! ATTTCAATATGTAAAAAAAA+AGG + Chr1:3267510-3267529 None:intergenic 10.0%
!!! AAAAAAAATAGAAATTTGTT+CGG + Chr1:3266859-3266878 None:intergenic 10.0%
!!! AACAATATTTTTTATGAAAT+TGG - Chr1:3265944-3265963 MsG0180000238.01.T01:intron 10.0%
!!! ATATTTTTTTGTTATATTGT+TGG + Chr1:3266377-3266396 None:intergenic 10.0%
!!! TAAAAAAAAAAAAAGAGTAT+TGG - Chr1:3268282-3268301 MsG0180000238.01.T01:intron 10.0%
!! AATATGTAAAAAAAAAGGTT+TGG + Chr1:3267505-3267524 None:intergenic 15.0%
!! AATTATCATTACATATAGAA+AGG + Chr1:3267583-3267602 None:intergenic 15.0%
!! ATATAATCAAATTCAAATCT+GGG + Chr1:3267308-3267327 None:intergenic 15.0%
!! TATATATATTAGCGAAAAAT+CGG - Chr1:3266190-3266209 MsG0180000238.01.T01:intron 15.0%
!!! AAGAAAAAAAAAAGATTTTG+AGG + Chr1:3266105-3266124 None:intergenic 15.0%
!!! ATTCTTTTTTCTGTATTTTT+TGG + Chr1:3265617-3265636 None:intergenic 15.0%
!!! ATTTTCTCTCATTTATTTTT+TGG - Chr1:3268363-3268382 MsG0180000238.01.T01:intron 15.0%
!!! GAAATTTTATAAACAAGATA+AGG + Chr1:3266911-3266930 None:intergenic 15.0%
!!! TAACAAAAAACAAATTTTGT+TGG - Chr1:3266984-3267003 MsG0180000238.01.T01:intron 15.0%
!!! TGACTATAATTTAGTTTTTA+AGG + Chr1:3268030-3268049 None:intergenic 15.0%
!!! TTTTCTCTCATTTATTTTTT+GGG - Chr1:3268364-3268383 MsG0180000238.01.T01:intron 15.0%
!!! TTTTGATCTATTTTAACATT+TGG - Chr1:3268455-3268474 MsG0180000238.01.T01:intron 15.0%
!! AAAAACTAAATTATAGTCAC+AGG - Chr1:3268031-3268050 MsG0180000238.01.T01:intron 20.0%
!! AAAACTAAATTATAGTCACA+GGG - Chr1:3268032-3268051 MsG0180000238.01.T01:intron 20.0%
!! AACCAAAAAAATAATTCATG+AGG + Chr1:3265408-3265427 None:intergenic 20.0%
!! AATTGTAAACTTTATTGTAG+AGG + Chr1:3264969-3264988 None:intergenic 20.0%
!! ACAAATAATACTTACAATGT+TGG + Chr1:3267776-3267795 None:intergenic 20.0%
!! ATACTAAGTTTATCCTAATT+AGG - Chr1:3265448-3265467 MsG0180000238.01.T01:intron 20.0%
!! ATCAAGCTAACATAAAAAAA+AGG + Chr1:3265895-3265914 None:intergenic 20.0%
!! ATTATAAACAAAGATGAAGA+TGG - Chr1:3268770-3268789 MsG0180000238.01.T01:CDS 20.0%
!! CTAAATCTATATACTTGAAA+AGG - Chr1:3267229-3267248 MsG0180000238.01.T01:intron 20.0%
!! GATATAATCAAATTCAAATC+TGG + Chr1:3267309-3267328 None:intergenic 20.0%
!! GATTTGAATTTGATTATATC+CGG - Chr1:3267309-3267328 MsG0180000238.01.T01:intron 20.0%
!! GTTCTTGAATATGATTTATT+TGG - Chr1:3265012-3265031 MsG0180000238.01.T01:CDS 20.0%
!! TAAAAGTTACAATTATTCCT+TGG - Chr1:3265500-3265519 MsG0180000238.01.T01:intron 20.0%
!! TAAACTTAGTATTTAAACGT+TGG + Chr1:3265440-3265459 None:intergenic 20.0%
!! TACTAAGTTTATCCTAATTA+GGG - Chr1:3265449-3265468 MsG0180000238.01.T01:intron 20.0%
!! TCAACTAAAAAGAGAAAATT+TGG - Chr1:3267403-3267422 MsG0180000238.01.T01:intron 20.0%
!! TTAACAAATGATCATATGTA+AGG - Chr1:3267869-3267888 MsG0180000238.01.T01:intron 20.0%
!! TTTCATTTATCCCTTAAAAA+GGG + Chr1:3266518-3266537 None:intergenic 20.0%
!!! AAAAAAAAAAGATTTTGAGG+TGG + Chr1:3266102-3266121 None:intergenic 20.0%
!!! AAATTTGCTAGCAATAATAA+TGG + Chr1:3266948-3266967 None:intergenic 20.0%
!!! ATGTGTTATTTATTTGTTGA+AGG - Chr1:3267810-3267829 MsG0180000238.01.T01:intron 20.0%
!!! CAAGTTGATTTATTGAATTT+GGG + Chr1:3268670-3268689 None:intergenic 20.0%
!!! GAAAAAACTCTTTTTTGTTA+AGG + Chr1:3268093-3268112 None:intergenic 20.0%
!!! GAAATTGGTTTTGATTAAAA+AGG - Chr1:3265959-3265978 MsG0180000238.01.T01:intron 20.0%
!!! TAATATTATGTTAGCTTGAT+TGG - Chr1:3265758-3265777 MsG0180000238.01.T01:intron 20.0%
!!! TCAAGTTGATTTATTGAATT+TGG + Chr1:3268671-3268690 None:intergenic 20.0%
!!! TTTTCATTTATCCCTTAAAA+AGG + Chr1:3266519-3266538 None:intergenic 20.0%
!!! TTTTCTGTATTTTTTGGATT+GGG + Chr1:3265611-3265630 None:intergenic 20.0%
!!! TTTTTCTGTATTTTTTGGAT+TGG + Chr1:3265612-3265631 None:intergenic 20.0%
! AAAAAATAATTCATGAGGTG+AGG + Chr1:3265403-3265422 None:intergenic 25.0%
! AAAAATAATTCATGAGGTGA+GGG + Chr1:3265402-3265421 None:intergenic 25.0%
! AAAAATTTCAAACCTTCAGA+TGG - Chr1:3264925-3264944 MsG0180000238.01.T01:CDS 25.0%
! AAGAGAAAAAACAAATACGA+CGG + Chr1:3265872-3265891 None:intergenic 25.0%
! AATATATCAACAAAATCCCT+GGG + Chr1:3267838-3267857 None:intergenic 25.0%
! AGATAATGCATTGTTATATC+CGG - Chr1:3267939-3267958 MsG0180000238.01.T01:intron 25.0%
! ATCTTTGATTAACAATGCTT+CGG - Chr1:3265317-3265336 MsG0180000238.01.T01:intron 25.0%
! ATTAGTAGCCAATTATAGTA+TGG + Chr1:3265742-3265761 None:intergenic 25.0%
! ATTTAAATTTCTGATGCGTT+TGG + Chr1:3268534-3268553 None:intergenic 25.0%
! GAAAAATCTAACAAAGAAGA+TGG - Chr1:3268740-3268759 MsG0180000238.01.T01:CDS 25.0%
! GTAAGTTACCATACTATAAT+TGG - Chr1:3265731-3265750 MsG0180000238.01.T01:intron 25.0%
! GTCATTGAAATAGATAATCA+TGG + Chr1:3266353-3266372 None:intergenic 25.0%
! TAACATTTGAACAAGATTAC+CGG + Chr1:3267331-3267350 None:intergenic 25.0%
! TAATATATCAACAAAATCCC+TGG + Chr1:3267839-3267858 None:intergenic 25.0%
! TCAAGAACATTGATATAGTT+GGG + Chr1:3265000-3265019 None:intergenic 25.0%
! TCAATAAATCAACTTGATAG+TGG - Chr1:3268674-3268693 MsG0180000238.01.T01:CDS 25.0%
! TGTTAATCAAAGATATGTCA+AGG + Chr1:3265311-3265330 None:intergenic 25.0%
! TTCAAGAACATTGATATAGT+TGG + Chr1:3265001-3265020 None:intergenic 25.0%
! TTTAAATTTCTGATGCGTTT+GGG + Chr1:3268533-3268552 None:intergenic 25.0%
!! ATTGAAATGATTCCCAATTT+TGG - Chr1:3268869-3268888 MsG0180000238.01.T01:CDS 25.0%
!! CTTAAACAAGCTTGTTTAAA+AGG + Chr1:3266058-3266077 None:intergenic 25.0%
!! CTTTTGCATTCTTGATTTTT+AGG - Chr1:3267624-3267643 MsG0180000238.01.T01:intron 25.0%
!! TTGAAAGTTGAGGATAATTT+TGG - Chr1:3266631-3266650 MsG0180000238.01.T01:intron 25.0%
!! TTTGGAACAAGAATGAAATT+TGG - Chr1:3267421-3267440 MsG0180000238.01.T01:intron 25.0%
!!! AAAGTGATTTTTTTCTGTGT+GGG - Chr1:3265792-3265811 MsG0180000238.01.T01:intron 25.0%
!!! AGTTTTTAAGGTGAATAAGT+AGG + Chr1:3268018-3268037 None:intergenic 25.0%
!!! CACCTCATGAATTATTTTTT+TGG - Chr1:3265403-3265422 MsG0180000238.01.T01:intron 25.0%
!!! GTTTTTAAGGTGAATAAGTA+GGG + Chr1:3268017-3268036 None:intergenic 25.0%
!!! TAAAGTGATTTTTTTCTGTG+TGG - Chr1:3265791-3265810 MsG0180000238.01.T01:intron 25.0%
!!! TAGTTTGTTACCCTTTTTAA+GGG - Chr1:3266505-3266524 MsG0180000238.01.T01:intron 25.0%
!!! TGGTTTTGATTAAAAAGGAT+AGG - Chr1:3265964-3265983 MsG0180000238.01.T01:intron 25.0%
!!! TTTGTCAACTATACTTTTTC+CGG + Chr1:3265196-3265215 None:intergenic 25.0%
AGAGAAAAAACAAATACGAC+GGG + Chr1:3265871-3265890 None:intergenic 30.0%
AGTATGGTAACTTACAGATA+TGG + Chr1:3265726-3265745 None:intergenic 30.0%
ATAGCAATGTTTGCCAAAAT+TGG + Chr1:3268885-3268904 None:intergenic 30.0%
CAATAAAAAGAGCGGAAAAA+TGG + Chr1:3266228-3266247 None:intergenic 30.0%
CCAGAAAATAAACCCTAATT+AGG + Chr1:3265464-3265483 None:intergenic 30.0%
GAACCAAAATGTCAATCAAA+TGG - Chr1:3267679-3267698 MsG0180000238.01.T01:intron 30.0%
GCTATTTGTATTGAAAGTTG+AGG - Chr1:3266621-3266640 MsG0180000238.01.T01:intron 30.0%
TAAATATGTGCACAATACCA+AGG + Chr1:3265520-3265539 None:intergenic 30.0%
TAGCAATGTTTGCCAAAATT+GGG + Chr1:3268884-3268903 None:intergenic 30.0%
TCATCAAGTGAGAATTTGAT+TGG + Chr1:3265646-3265665 None:intergenic 30.0%
TGGAAATATCAAGATTGACT+TGG - Chr1:3268694-3268713 MsG0180000238.01.T01:CDS 30.0%
TTTATCATGTTCAACTTTGC+TGG + Chr1:3267476-3267495 None:intergenic 30.0%
! AGATTTTTCCTCTGTTGTAT+TGG + Chr1:3268729-3268748 None:intergenic 30.0%
! ATTGTTGTTGTTGTTGTTGT+TGG - Chr1:3266785-3266804 MsG0180000238.01.T01:intron 30.0%
! GTTTCTTTGTGATTTTTCTG+TGG - Chr1:3266819-3266838 MsG0180000238.01.T01:intron 30.0%
! TTGATTCGTACAATTGGAAT+TGG - Chr1:3265150-3265169 MsG0180000238.01.T01:CDS 30.0%
! TTTTTCCGCTCTTTTTATTG+CGG - Chr1:3266228-3266247 MsG0180000238.01.T01:intron 30.0%
!! CCTAATTAGGGTTTATTTTC+TGG - Chr1:3265461-3265480 MsG0180000238.01.T01:intron 30.0%
!!! CTAGTTTGTTACCCTTTTTA+AGG - Chr1:3266504-3266523 MsG0180000238.01.T01:intron 30.0%
!!! TTTTTTGGTTACATGGATGT+CGG - Chr1:3267975-3267994 MsG0180000238.01.T01:intron 30.0%
AAAGAATCCTTGGGAGATTT+GGG - Chr1:3265041-3265060 MsG0180000238.01.T01:CDS 35.0%
AGAACAATGATGTTTGGTTG+GGG - Chr1:3268633-3268652 MsG0180000238.01.T01:CDS 35.0%
AGTATAGTTGACAAAGAGAC+TGG - Chr1:3265201-3265220 MsG0180000238.01.T01:intron 35.0%
AGTGGGAGATTTCTTCTTTA+AGG + Chr1:3265121-3265140 None:intergenic 35.0%
ATGAAGATGGTGATATCTCT+TGG - Chr1:3268783-3268802 MsG0180000238.01.T01:CDS 35.0%
CCAAAATGTCAATCAAATGG+TGG - Chr1:3267682-3267701 MsG0180000238.01.T01:intron 35.0%
GAGAAAAAACAAATACGACG+GGG + Chr1:3265870-3265889 None:intergenic 35.0%
GAGAACAATGATGTTTGGTT+GGG - Chr1:3268632-3268651 MsG0180000238.01.T01:CDS 35.0%
TACAATTGGAATTGGTACGT+GGG - Chr1:3265158-3265177 MsG0180000238.01.T01:CDS 35.0%
TCTCACTTGATGAATAGCAT+TGG - Chr1:3265653-3265672 MsG0180000238.01.T01:intron 35.0%
TCTGCATAGAACATAATTGC+TGG + Chr1:3265570-3265589 None:intergenic 35.0%
TGCTGGTCAGCTAAAAAATT+TGG + Chr1:3267459-3267478 None:intergenic 35.0%
TGGTATCACAAAGAATCCTT+GGG - Chr1:3265032-3265051 MsG0180000238.01.T01:CDS 35.0%
TTGGTATCACAAAGAATCCT+TGG - Chr1:3265031-3265050 MsG0180000238.01.T01:CDS 35.0%
! AAACGCTTGATTCGTACAAT+TGG - Chr1:3265144-3265163 MsG0180000238.01.T01:CDS 35.0%
! AAGAGTAACACTTCTGAAGA+TGG - Chr1:3265261-3265280 MsG0180000238.01.T01:intron 35.0%
! AAGTGTTACTCTTCTCAAAG+CGG + Chr1:3265254-3265273 None:intergenic 35.0%
! AATGCAAGGGCACAATAATA+AGG + Chr1:3268601-3268620 None:intergenic 35.0%
! ATAACATGCTTTGCCTCTTT+TGG + Chr1:3266674-3266693 None:intergenic 35.0%
! CCACCATTTGATTGACATTT+TGG + Chr1:3267685-3267704 None:intergenic 35.0%
! TAACATGCTTTGCCTCTTTT+GGG + Chr1:3266673-3266692 None:intergenic 35.0%
! TTATTTGTTGAAGGAACCCA+GGG - Chr1:3267819-3267838 MsG0180000238.01.T01:intron 35.0%
! TTTAGGACATCATTGTTTCG+AGG - Chr1:3267641-3267660 MsG0180000238.01.T01:intron 35.0%
! TTTATTTGTTGAAGGAACCC+AGG - Chr1:3267818-3267837 MsG0180000238.01.T01:intron 35.0%
!! AATCAAATGGTGGACACAAA+AGG - Chr1:3267692-3267711 MsG0180000238.01.T01:intron 35.0%
!! TAAAAGTGCCAATACAACAG+AGG - Chr1:3268718-3268737 MsG0180000238.01.T01:CDS 35.0%
!!! GAAACGTTTTTTTAGTCCGA+GGG + Chr1:3265232-3265251 None:intergenic 35.0%
!!! GGGTTCTTTTTTTGGTTACA+TGG - Chr1:3267968-3267987 MsG0180000238.01.T01:intron 35.0%
!!! TTTTTGGTTACATGGATGTC+GGG - Chr1:3267976-3267995 MsG0180000238.01.T01:intron 35.0%
AAAAAAAGAACCCCGACATC+CGG + Chr1:3267961-3267980 None:intergenic 40.0%
AATAATTCATGAGGTGAGGG+TGG + Chr1:3265399-3265418 None:intergenic 40.0%
ACATTGATATAGTTGGGCAC+TGG + Chr1:3264994-3265013 None:intergenic 40.0%
ACCAAGTGATCTATGCACAT+GGG + Chr1:3267917-3267936 None:intergenic 40.0%
ACGAATCAAGCGTTTACTAG+TGG + Chr1:3265139-3265158 None:intergenic 40.0%
AGAAAAAGTCAGCCCAAAAG+AGG - Chr1:3266658-3266677 MsG0180000238.01.T01:intron 40.0%
ATTGTTATATCCGGATGTCG+GGG - Chr1:3267948-3267967 MsG0180000238.01.T01:intron 40.0%
CAAAGAATCCTTGGGAGATT+TGG - Chr1:3265040-3265059 MsG0180000238.01.T01:CDS 40.0%
CATCACCGCAATAAAAAGAG+CGG + Chr1:3266236-3266255 None:intergenic 40.0%
CATTGTTATATCCGGATGTC+GGG - Chr1:3267947-3267966 MsG0180000238.01.T01:intron 40.0%
CGAATCAAGCGTTTACTAGT+GGG + Chr1:3265138-3265157 None:intergenic 40.0%
GAACTCTTAGAAGGGAATGA+AGG - Chr1:3268824-3268843 MsG0180000238.01.T01:CDS 40.0%
GCATTGTTATATCCGGATGT+CGG - Chr1:3267946-3267965 MsG0180000238.01.T01:intron 40.0%
GGAGAACAATGATGTTTGGT+TGG - Chr1:3268631-3268650 MsG0180000238.01.T01:CDS 40.0%
GTACAATTGGAATTGGTACG+TGG - Chr1:3265157-3265176 MsG0180000238.01.T01:CDS 40.0%
TACCAAGTGATCTATGCACA+TGG + Chr1:3267918-3267937 None:intergenic 40.0%
TCCTGCAAATGAGAATGCAA+GGG + Chr1:3268614-3268633 None:intergenic 40.0%
TGAAGGAAGCTTAATCGAAG+AGG - Chr1:3268841-3268860 MsG0180000238.01.T01:CDS 40.0%
TGCAGGAGAACAATGATGTT+TGG - Chr1:3268627-3268646 MsG0180000238.01.T01:CDS 40.0%
TTGCAAGGGAACTCTTAGAA+GGG - Chr1:3268816-3268835 MsG0180000238.01.T01:CDS 40.0%
TTTGCAAGGGAACTCTTAGA+AGG - Chr1:3268815-3268834 MsG0180000238.01.T01:CDS 40.0%
! AGTAACACTTCTGAAGATGG+TGG - Chr1:3265264-3265283 MsG0180000238.01.T01:intron 40.0%
! CAAGGAAAGACAGTTGTTTC+CGG - Chr1:3265686-3265705 MsG0180000238.01.T01:intron 40.0%
! GTTGTTGTTGTTGTTGTTGG+TGG - Chr1:3266788-3266807 MsG0180000238.01.T01:intron 40.0%
! TGAATAGCATTGGACACGAT+AGG - Chr1:3265663-3265682 MsG0180000238.01.T01:intron 40.0%
!! AGCTTTAAGTCCAAGAACCA+CGG + Chr1:3267749-3267768 None:intergenic 40.0%
!! TTTTGGTTACATGGATGTCG+GGG - Chr1:3267977-3267996 MsG0180000238.01.T01:intron 40.0%
!!! CTGCTTTTGCGTTTTTTTGC+AGG - Chr1:3265544-3265563 MsG0180000238.01.T01:intron 40.0%
!!! GGAAACGTTTTTTTAGTCCG+AGG + Chr1:3265233-3265252 None:intergenic 40.0%
!!! GTTTTTTTAGTCCGAGGGTT+TGG + Chr1:3265227-3265246 None:intergenic 40.0%
AACTCTTCCCAAATCTCCCA+AGG + Chr1:3265051-3265070 None:intergenic 45.0%
AGGAAGCTTAATCGAAGAGG+AGG - Chr1:3268844-3268863 MsG0180000238.01.T01:CDS 45.0%
AGTTCCCTTGCAAAAAGCTC+AGG + Chr1:3268809-3268828 None:intergenic 45.0%
CCAAGTGATCTATGCACATG+GGG + Chr1:3267916-3267935 None:intergenic 45.0%
CGAAGAAGTTCACCATCTGA+AGG + Chr1:3264940-3264959 None:intergenic 45.0%
CTCCTGCAAATGAGAATGCA+AGG + Chr1:3268615-3268634 None:intergenic 45.0%
GGACACAAAAGGAGAAAGGT+GGG - Chr1:3267703-3267722 MsG0180000238.01.T01:intron 45.0%
GGAGAAAGGTGGGAAATACT+TGG - Chr1:3267713-3267732 MsG0180000238.01.T01:intron 45.0%
TGGACACAAAAGGAGAAAGG+TGG - Chr1:3267702-3267721 MsG0180000238.01.T01:intron 45.0%
! TGGTGGACACAAAAGGAGAA+AGG - Chr1:3267699-3267718 MsG0180000238.01.T01:intron 45.0%
! TTGGCCTGAGCTTTTTGCAA+GGG - Chr1:3268802-3268821 MsG0180000238.01.T01:CDS 45.0%
!! ACACTTCTGAAGATGGTGGA+TGG - Chr1:3265268-3265287 MsG0180000238.01.T01:intron 45.0%
!!! GGATGTCGGGGTTCTTTTTT+TGG - Chr1:3267960-3267979 MsG0180000238.01.T01:intron 45.0%
!!! GTTTTATTTATTTATTTTTT+TGG - Chr1:3267045-3267064 MsG0180000238.01.T01:intron 5.0%
!!! TTTTATTTAAATACAAAATT+TGG - Chr1:3267372-3267391 MsG0180000238.01.T01:intron 5.0%
!!! TTTTGTATTTAAATAAAATT+TGG + Chr1:3267370-3267389 None:intergenic 5.0%
CCAGAAAAGACCGTGGTTCT+TGG - Chr1:3267736-3267755 MsG0180000238.01.T01:intron 50.0%
CCCCATGTGCATAGATCACT+TGG - Chr1:3267913-3267932 MsG0180000238.01.T01:intron 50.0%
GCCCTTGCATTCTCATTTGC+AGG - Chr1:3268610-3268629 MsG0180000238.01.T01:CDS 50.0%
TACTTGGCCAGAAAAGACCG+TGG - Chr1:3267729-3267748 MsG0180000238.01.T01:intron 50.0%
! AAAGAGACTGGCCAAACCCT+CGG - Chr1:3265213-3265232 MsG0180000238.01.T01:intron 50.0%
! ACAGATATGGCGTTTTCCGC+AGG + Chr1:3265713-3265732 None:intergenic 50.0%
! AGCATTGGACACGATAGGCA+AGG - Chr1:3265668-3265687 MsG0180000238.01.T01:intron 50.0%
! CCAAGAACCACGGTCTTTTC+TGG + Chr1:3267739-3267758 None:intergenic 50.0%
! CTTGGCCTGAGCTTTTTGCA+AGG - Chr1:3268801-3268820 MsG0180000238.01.T01:CDS 50.0%
ACGTGGGAAGCTGAAGACAC+CGG - Chr1:3265174-3265193 MsG0180000238.01.T01:intron 55.0%
! GACAGTTGTTTCCGGACCTG+CGG - Chr1:3265694-3265713 MsG0180000238.01.T01:intron 55.0%
! TATGGCGTTTTCCGCAGGTC+CGG + Chr1:3265708-3265727 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 3264901 3268910 3264901 ID=MsG0180000238.01;Name=MsG0180000238.01
Chr1 mRNA 3264901 3268910 3264901 ID=MsG0180000238.01.T01;Parent=MsG0180000238.01;Name=MsG0180000238.01.T01;_AED=0.29;_eAED=0.29;_QI=0|0|0|0.75|1|1|4|0|338
Chr1 exon 3268473 3268910 3268473 ID=MsG0180000238.01.T01:exon:6173;Parent=MsG0180000238.01.T01
Chr1 exon 3268081 3268245 3268081 ID=MsG0180000238.01.T01:exon:6174;Parent=MsG0180000238.01.T01
Chr1 exon 3266031 3266165 3266031 ID=MsG0180000238.01.T01:exon:6175;Parent=MsG0180000238.01.T01
Chr1 exon 3264901 3265179 3264901 ID=MsG0180000238.01.T01:exon:6176;Parent=MsG0180000238.01.T01
Chr1 CDS 3268473 3268910 3268473 ID=MsG0180000238.01.T01:cds;Parent=MsG0180000238.01.T01
Chr1 CDS 3268081 3268245 3268081 ID=MsG0180000238.01.T01:cds;Parent=MsG0180000238.01.T01
Chr1 CDS 3266031 3266165 3266031 ID=MsG0180000238.01.T01:cds;Parent=MsG0180000238.01.T01
Chr1 CDS 3264901 3265179 3264901 ID=MsG0180000238.01.T01:cds;Parent=MsG0180000238.01.T01
Gene Sequence

>MsG0180000238.01.T01

ATGGCTCACAATTCTCGATCAAGTAAAAATTTCAAACCTTCAGATGGTGAACTTCTTCGATCATTCCTCTACAATAAAGTTTACAATTTACCAGTGCCCAACTATATCAATGTTCTTGAATATGATTTATTTGGTATCACAAAGAATCCTTGGGAGATTTGGGAAGAGTTTTCAAGTTCTCACTCATATCATGAAAAAGACTTATATTTCTTCACTACCTTAAAGAAGAAATCTCCCACTAGTAAACGCTTGATTCGTACAATTGGAATTGGTACGTGGGAAGCTGAAGACACCGGAAAAAGTATAGTTGACAAAGAGACTGGCCAAACCCTCGGACTAAAAAAACGTTTCCGCTTTGAGAAGAGTAACACTTCTGAAGATGGTGGATGGATCTTGCATGAATATAGCCTTGACATATCTTTGATTAACAATGCTTCGGCCAGCAATTATGTTCTATGCAGATTCAGAAAGAATTTGAAACCCAATCCAAAAAATACAGAAAAAAGAATCGATAACCAATCAAATTCTCACTTGATGAATAGCATTGGACACGATAGGCAAGGAAAGACAGTTGTTTCCGGACCTGCGGAAAACGCCATATCTGACATCATTGTTTCGAGGAAATTTGTTCATGAAGAACCAAAATGTCAATCAAATGGTGGACACAAAAGGAGAAAGGTGGGAAATACTTGGCCAGAAAAGACCGTGGTTCTTGGACTTAAAGCTATCACCAACATTGAGAACAATGATGTTTGGTTGGGGCAAAATATTGACCCAAATTCAATAAATCAACTTGATAGTGGAAATATCAAGATTGACTTGGTTAAAAGTGCCAATACAACAGAGGAAAAATCTAACAAAGAAGATGGTGATCATATTATAAACAAAGATGAAGATGGTGATATCTCTTGGCCTGAGCTTTTTGCAAGGGAACTCTTAGAAGGGAATGAAGGAAGCTTAATCGAAGAGGAGGATATTGAAATGATTCCCAATTTTGGCAAACATTGCTATGTTTAA
Protein sequence

>MsG0180000238.01.T01

MAHNSRSSKNFKPSDGELLRSFLYNKVYNLPVPNYINVLEYDLFGITKNPWEIWEEFSSSHSYHEKDLYFFTTLKKKSPTSKRLIRTIGIGTWEAEDTGKSIVDKETGQTLGLKKRFRFEKSNTSEDGGWILHEYSLDISLINNASASNYVLCRFRKNLKPNPKNTEKRIDNQSNSHLMNSIGHDRQGKTVVSGPAENAISDIIVSRKFVHEEPKCQSNGGHKRRKVGNTWPEKTVVLGLKAITNIENNDVWLGQNIDPNSINQLDSGNIKIDLVKSANTTEEKSNKEDGDHIINKDEDGDISWPELFARELLEGNEGSLIEEEDIEMIPNFGKHCYV*