Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000344.01.T01 | KEH40004.1 | 97.241 | 145 | 4 | 0 | 1 | 145 | 1 | 145 | 1.10E-97 | 287 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000344.01.T01 | Q9SI15 | 43.75 | 80 | 45 | 0 | 45 | 124 | 16 | 95 | 5.74E-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000344.01.T01 | A0A072VDB1 | 97.241 | 145 | 4 | 0 | 1 | 145 | 1 | 145 | 5.25e-98 | 287 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180000344.01.T01 | TF | bZIP |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000344.01.T01 | MTR_1g017570 | 97.241 | 145 | 4 | 0 | 1 | 145 | 1 | 145 | 1.33e-101 | 287 |
| MsG0180000344.01.T01 | MTR_3g103360 | 58.333 | 144 | 56 | 2 | 1 | 144 | 1 | 140 | 8.07e-50 | 156 |
| MsG0180000344.01.T01 | MTR_4g097440 | 47.154 | 123 | 47 | 3 | 22 | 128 | 23 | 143 | 8.70e-20 | 80.9 |
| MsG0180000344.01.T01 | MTR_5g015090 | 45.082 | 122 | 51 | 3 | 31 | 139 | 22 | 140 | 6.09e-18 | 75.9 |
| MsG0180000344.01.T01 | MTR_8g086020 | 41.584 | 101 | 53 | 2 | 45 | 143 | 18 | 114 | 1.14e-14 | 67.4 |
| MsG0180000344.01.T01 | MTR_1g087040 | 37.273 | 110 | 62 | 2 | 34 | 143 | 4 | 106 | 1.68e-13 | 63.9 |
| MsG0180000344.01.T01 | MTR_1g022495 | 41.111 | 90 | 51 | 1 | 35 | 124 | 7 | 94 | 2.43e-12 | 61.2 |
| MsG0180000344.01.T01 | MTR_3g117120 | 43.902 | 82 | 44 | 1 | 43 | 124 | 15 | 94 | 2.68e-12 | 60.8 |
| MsG0180000344.01.T01 | MTR_4g070860 | 43.750 | 80 | 43 | 1 | 45 | 124 | 17 | 94 | 3.20e-12 | 60.8 |
| MsG0180000344.01.T01 | MTR_7g029400 | 44.156 | 77 | 43 | 0 | 59 | 135 | 83 | 159 | 1.56e-11 | 59.7 |
| MsG0180000344.01.T01 | MTR_8g015250 | 42.857 | 77 | 44 | 0 | 59 | 135 | 73 | 149 | 2.32e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000344.01.T01 | AT3G49760 | 38.849 | 139 | 77 | 2 | 9 | 139 | 13 | 151 | 1.30e-20 | 82.8 |
| MsG0180000344.01.T01 | AT2G22850 | 45.783 | 83 | 45 | 0 | 57 | 139 | 126 | 208 | 2.31e-15 | 70.5 |
| MsG0180000344.01.T01 | AT2G22850 | 45.783 | 83 | 45 | 0 | 57 | 139 | 126 | 208 | 2.31e-15 | 70.5 |
| MsG0180000344.01.T01 | AT2G18160 | 43.750 | 80 | 45 | 0 | 45 | 124 | 16 | 95 | 5.85e-14 | 65.9 |
| MsG0180000344.01.T01 | AT1G75390 | 41.667 | 84 | 49 | 0 | 45 | 128 | 26 | 109 | 3.30e-13 | 63.9 |
| MsG0180000344.01.T01 | AT4G37730 | 51.471 | 68 | 33 | 0 | 57 | 124 | 194 | 261 | 3.94e-13 | 65.5 |
| MsG0180000344.01.T01 | AT1G75390 | 41.463 | 82 | 48 | 0 | 43 | 124 | 24 | 105 | 1.01e-12 | 61.2 |
| MsG0180000344.01.T01 | AT1G59530 | 39.344 | 122 | 68 | 2 | 18 | 139 | 14 | 129 | 1.02e-11 | 59.3 |
| MsG0180000344.01.T01 | AT5G15830 | 38.835 | 103 | 59 | 2 | 26 | 128 | 44 | 142 | 1.02e-11 | 60.1 |
Find 45 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCCCTCATGGCAACACTTT+TGG | 0.232653 | 1:+4694293 | MsG0180000344.01.T01:CDS |
| CGATTTGAACCGGTTGAGAA+TGG | 0.382249 | 1:+4694179 | MsG0180000344.01.T01:CDS |
| TTGTGAACCGGACCCTGGAC+TGG | 0.414459 | 1:-4694037 | None:intergenic |
| ACCGGTTGAGAATGGAAAAC+CGG | 0.420192 | 1:+4694187 | MsG0180000344.01.T01:CDS |
| GGTCCGGCTCACGTGTGTTC+CGG | 0.430584 | 1:+4693942 | MsG0180000344.01.T01:CDS |
| TAAGGAGAATGCAATCAAAC+CGG | 0.448119 | 1:+4694100 | MsG0180000344.01.T01:CDS |
| ACCTCACGAACGATTTGAAC+CGG | 0.459693 | 1:+4694169 | MsG0180000344.01.T01:CDS |
| ATTGCATGGTTAACCCTAGC+TGG | 0.474346 | 1:-4694221 | None:intergenic |
| GGACCGGATACTCCTCTTCA+TGG | 0.477097 | 1:-4693924 | None:intergenic |
| ACGACTTGATCCTTGTGAAC+CGG | 0.478619 | 1:-4694049 | None:intergenic |
| GTGAACATGGTTTCATGAAC+CGG | 0.479700 | 1:-4693961 | None:intergenic |
| CCTGGACTGGATGGATCAAC+CGG | 0.484260 | 1:-4694024 | None:intergenic |
| CCGGTTGATCCATCCAGTCC+AGG | 0.489171 | 1:+4694024 | MsG0180000344.01.T01:CDS |
| GATCCTTGTGAACCGGACCC+TGG | 0.490890 | 1:-4694042 | None:intergenic |
| GATCAACCGGTTGGTTAATG+AGG | 0.507162 | 1:-4694011 | None:intergenic |
| GAACCGGAACACACGTGAGC+CGG | 0.519769 | 1:-4693945 | None:intergenic |
| GATCCATCCAGTCCAGGGTC+CGG | 0.524687 | 1:+4694030 | MsG0180000344.01.T01:CDS |
| CTTCCATGAAGAGGAGTATC+CGG | 0.526400 | 1:+4693921 | MsG0180000344.01.T01:CDS |
| GGAACACACGTGAGCCGGAC+CGG | 0.528631 | 1:-4693940 | None:intergenic |
| GTTCACACAAGGCGAAATAG+AGG | 0.540753 | 1:+4693978 | MsG0180000344.01.T01:CDS |
| ATCCAAAAGTGTTGCCATGA+GGG | 0.543780 | 1:-4694295 | None:intergenic |
| TATTTCGCCTTGTGTGAACA+TGG | 0.548387 | 1:-4693974 | None:intergenic |
| CCGGTTGAGAATGGAAAACC+GGG | 0.557045 | 1:+4694188 | MsG0180000344.01.T01:CDS |
| AATCAAACCGGGAGTCGGCT+AGG | 0.560261 | 1:+4694112 | MsG0180000344.01.T01:CDS |
| ATCAGAATCAATGTCCCTCA+TGG | 0.566320 | 1:+4694281 | MsG0180000344.01.T01:CDS |
| AGCAATGTCCTTCCATGAAG+AGG | 0.576455 | 1:+4693912 | None:intergenic |
| GAACCGGACCCTGGACTGGA+TGG | 0.582397 | 1:-4694033 | None:intergenic |
| ATGAAGAGGAGTATCCGGTC+CGG | 0.588642 | 1:+4693926 | MsG0180000344.01.T01:CDS |
| AGTCCAGGGTCCGGTTCACA+AGG | 0.591544 | 1:+4694039 | MsG0180000344.01.T01:CDS |
| ACCGGTTCAAATCGTTCGTG+AGG | 0.594738 | 1:-4694170 | None:intergenic |
| CGGATACTCCTCTTCATGGA+AGG | 0.596073 | 1:-4693920 | None:intergenic |
| CGGTTGATCCATCCAGTCCA+GGG | 0.598027 | 1:+4694025 | MsG0180000344.01.T01:CDS |
| GACTGGATGGATCAACCGGT+TGG | 0.601414 | 1:-4694020 | None:intergenic |
| GTGACCGCCTAGCCGACTCC+CGG | 0.602249 | 1:-4694119 | None:intergenic |
| TAATATGAGATTGTATTGCA+TGG | 0.609971 | 1:-4694235 | None:intergenic |
| ATCAACCGGTTGGTTAATGA+GGG | 0.611428 | 1:-4694010 | None:intergenic |
| AATGCAATCAAACCGGGAGT+CGG | 0.621410 | 1:+4694107 | MsG0180000344.01.T01:CDS |
| ATACAATCTCATATTATCAA+CGG | 0.623568 | 1:+4694242 | MsG0180000344.01.T01:CDS |
| AAGGAGAATGCAATCAAACC+GGG | 0.631263 | 1:+4694101 | MsG0180000344.01.T01:CDS |
| CATGAAACCATGTTCACACA+AGG | 0.636957 | 1:+4693967 | MsG0180000344.01.T01:CDS |
| GATCCAAAAGTGTTGCCATG+AGG | 0.664933 | 1:-4694296 | None:intergenic |
| TTCACAAGGATCAAGTCGTG+CGG | 0.675020 | 1:+4694053 | MsG0180000344.01.T01:CDS |
| AGTCGGCTAGGCGGTCACGT+TGG | 0.676204 | 1:+4694124 | MsG0180000344.01.T01:CDS |
| GGTCACGTTGGAGAAAGAAG+CGG | 0.707696 | 1:+4694136 | MsG0180000344.01.T01:CDS |
| CAAACCGGGAGTCGGCTAGG+CGG | 0.728540 | 1:+4694115 | MsG0180000344.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATACAATCTCATATTATCAA+CGG | + | Chr1:4694242-4694261 | MsG0180000344.01.T01:CDS | 20.0% |
| ! | TAATATGAGATTGTATTGCA+TGG | - | Chr1:4694238-4694257 | None:intergenic | 25.0% |
| ! | CAACACTTTTGGATCTATAT+CGG | + | Chr1:4694304-4694323 | MsG0180000344.01.T01:CDS | 30.0% |
| !!! | TTCTCCTTATTTTTCTCTCA+CGG | - | Chr1:4694089-4694108 | None:intergenic | 30.0% |
| TAAGGAGAATGCAATCAAAC+CGG | + | Chr1:4694100-4694119 | MsG0180000344.01.T01:CDS | 35.0% | |
| ! | ATTTTCCCTCATTAACCAAC+CGG | + | Chr1:4694005-4694024 | MsG0180000344.01.T01:CDS | 35.0% |
| !!! | TCTCCTTATTTTTCTCTCAC+GGG | - | Chr1:4694088-4694107 | None:intergenic | 35.0% |
| AAGGAGAATGCAATCAAACC+GGG | + | Chr1:4694101-4694120 | MsG0180000344.01.T01:CDS | 40.0% | |
| ATCAGAATCAATGTCCCTCA+TGG | + | Chr1:4694281-4694300 | MsG0180000344.01.T01:CDS | 40.0% | |
| CATGAAACCATGTTCACACA+AGG | + | Chr1:4693967-4693986 | MsG0180000344.01.T01:CDS | 40.0% | |
| GGGAACTAAAAAACCAGCTA+GGG | + | Chr1:4694208-4694227 | MsG0180000344.01.T01:CDS | 40.0% | |
| GTGAACATGGTTTCATGAAC+CGG | - | Chr1:4693964-4693983 | None:intergenic | 40.0% | |
| TATTTCGCCTTGTGTGAACA+TGG | - | Chr1:4693977-4693996 | None:intergenic | 40.0% | |
| ! | ATCAACCGGTTGGTTAATGA+GGG | - | Chr1:4694013-4694032 | None:intergenic | 40.0% |
| !! | ATCCAAAAGTGTTGCCATGA+GGG | - | Chr1:4694298-4694317 | None:intergenic | 40.0% |
| !!! | CTAGCTGGTTTTTTAGTTCC+CGG | - | Chr1:4694209-4694228 | None:intergenic | 40.0% |
| AATGCAATCAAACCGGGAGT+CGG | + | Chr1:4694107-4694126 | MsG0180000344.01.T01:CDS | 45.0% | |
| ACCGGTTGAGAATGGAAAAC+CGG | + | Chr1:4694187-4694206 | MsG0180000344.01.T01:CDS | 45.0% | |
| ACCTCACGAACGATTTGAAC+CGG | + | Chr1:4694169-4694188 | MsG0180000344.01.T01:CDS | 45.0% | |
| ACGACTTGATCCTTGTGAAC+CGG | - | Chr1:4694052-4694071 | None:intergenic | 45.0% | |
| ATTGCATGGTTAACCCTAGC+TGG | - | Chr1:4694224-4694243 | None:intergenic | 45.0% | |
| CGACCCGTGAGAGAAAAATA+AGG | + | Chr1:4694082-4694101 | MsG0180000344.01.T01:CDS | 45.0% | |
| CGATTTGAACCGGTTGAGAA+TGG | + | Chr1:4694179-4694198 | MsG0180000344.01.T01:CDS | 45.0% | |
| CGGGAACTAAAAAACCAGCT+AGG | + | Chr1:4694207-4694226 | MsG0180000344.01.T01:CDS | 45.0% | |
| GTTCACACAAGGCGAAATAG+AGG | + | Chr1:4693978-4693997 | MsG0180000344.01.T01:CDS | 45.0% | |
| TTCACAAGGATCAAGTCGTG+CGG | + | Chr1:4694053-4694072 | MsG0180000344.01.T01:CDS | 45.0% | |
| ! | GATCAACCGGTTGGTTAATG+AGG | - | Chr1:4694014-4694033 | None:intergenic | 45.0% |
| !! | CTTCCATGAAGAGGAGTATC+CGG | + | Chr1:4693921-4693940 | MsG0180000344.01.T01:CDS | 45.0% |
| !! | GATCCAAAAGTGTTGCCATG+AGG | - | Chr1:4694299-4694318 | None:intergenic | 45.0% |
| CCGGTTGAGAATGGAAAACC+GGG | + | Chr1:4694188-4694207 | MsG0180000344.01.T01:CDS | 50.0% | |
| CGGATACTCCTCTTCATGGA+AGG | - | Chr1:4693923-4693942 | None:intergenic | 50.0% | |
| GGTCACGTTGGAGAAAGAAG+CGG | + | Chr1:4694136-4694155 | MsG0180000344.01.T01:CDS | 50.0% | |
| ! | ACCGGTTCAAATCGTTCGTG+AGG | - | Chr1:4694173-4694192 | None:intergenic | 50.0% |
| ! | CCCGGTTTTCCATTCTCAAC+CGG | - | Chr1:4694191-4694210 | None:intergenic | 50.0% |
| ! | GTCCCTCATGGCAACACTTT+TGG | + | Chr1:4694293-4694312 | MsG0180000344.01.T01:CDS | 50.0% |
| !! | ATGAAGAGGAGTATCCGGTC+CGG | + | Chr1:4693926-4693945 | MsG0180000344.01.T01:CDS | 50.0% |
| AATCAAACCGGGAGTCGGCT+AGG | + | Chr1:4694112-4694131 | MsG0180000344.01.T01:CDS | 55.0% | |
| CGGTTGATCCATCCAGTCCA+GGG | + | Chr1:4694025-4694044 | MsG0180000344.01.T01:CDS | 55.0% | |
| GACTGGATGGATCAACCGGT+TGG | - | Chr1:4694023-4694042 | None:intergenic | 55.0% | |
| GGACCGGATACTCCTCTTCA+TGG | - | Chr1:4693927-4693946 | None:intergenic | 55.0% | |
| ! | CCTGGACTGGATGGATCAAC+CGG | - | Chr1:4694027-4694046 | None:intergenic | 55.0% |
| AGTCCAGGGTCCGGTTCACA+AGG | + | Chr1:4694039-4694058 | MsG0180000344.01.T01:CDS | 60.0% | |
| CCGGTTGATCCATCCAGTCC+AGG | + | Chr1:4694024-4694043 | MsG0180000344.01.T01:CDS | 60.0% | |
| GAACCGGAACACACGTGAGC+CGG | - | Chr1:4693948-4693967 | None:intergenic | 60.0% | |
| GATCCATCCAGTCCAGGGTC+CGG | + | Chr1:4694030-4694049 | MsG0180000344.01.T01:CDS | 60.0% | |
| GATCCTTGTGAACCGGACCC+TGG | - | Chr1:4694045-4694064 | None:intergenic | 60.0% | |
| TTGTGAACCGGACCCTGGAC+TGG | - | Chr1:4694040-4694059 | None:intergenic | 60.0% | |
| AGTCGGCTAGGCGGTCACGT+TGG | + | Chr1:4694124-4694143 | MsG0180000344.01.T01:CDS | 65.0% | |
| CAAACCGGGAGTCGGCTAGG+CGG | + | Chr1:4694115-4694134 | MsG0180000344.01.T01:CDS | 65.0% | |
| GGAACACACGTGAGCCGGAC+CGG | - | Chr1:4693943-4693962 | None:intergenic | 65.0% | |
| GGTCCGGCTCACGTGTGTTC+CGG | + | Chr1:4693942-4693961 | MsG0180000344.01.T01:CDS | 65.0% | |
| ! | GAACCGGACCCTGGACTGGA+TGG | - | Chr1:4694036-4694055 | None:intergenic | 65.0% |
| ! | GTGACCGCCTAGCCGACTCC+CGG | - | Chr1:4694122-4694141 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 4693916 | 4694353 | 4693916 | ID=MsG0180000344.01;Name=MsG0180000344.01 |
| Chr1 | mRNA | 4693916 | 4694353 | 4693916 | ID=MsG0180000344.01.T01;Parent=MsG0180000344.01;Name=MsG0180000344.01.T01;_AED=0.34;_eAED=0.34;_QI=0|-1|0|1|-1|1|1|0|145 |
| Chr1 | exon | 4693916 | 4694353 | 4693916 | ID=MsG0180000344.01.T01:exon:475;Parent=MsG0180000344.01.T01 |
| Chr1 | CDS | 4693916 | 4694353 | 4693916 | ID=MsG0180000344.01.T01:cds;Parent=MsG0180000344.01.T01 |
| Gene Sequence |
| Protein sequence |
