AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000565.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000565.01.T01 MTR_1g013170 96.310 271 10 0 1 271 1 271 1.54e-170 482
MsG0180000565.01.T01 MTR_7g117705 80.524 267 50 2 1 266 1 266 3.42e-142 409
MsG0180000565.01.T01 MTR_1g013180 80.443 271 46 1 1 271 152 415 5.86e-142 416
MsG0180000565.01.T01 MTR_3g106220 69.091 275 78 3 2 271 5 277 4.33e-123 366
MsG0180000565.01.T01 MTR_2g450070 58.696 276 102 3 3 269 16 288 1.51e-98 303
MsG0180000565.01.T01 MTR_2g450070 58.696 276 102 3 3 269 16 288 2.77e-98 303
MsG0180000565.01.T01 MTR_4g121020 53.169 284 100 4 2 271 28 292 2.13e-94 293
MsG0180000565.01.T01 MTR_4g121020 53.169 284 100 4 2 271 28 292 2.69e-94 293
MsG0180000565.01.T01 MTR_2g034960 49.825 285 119 2 1 275 25 295 9.94e-93 287
MsG0180000565.01.T01 MTR_1g032570 53.846 260 115 2 4 259 10 268 5.02e-91 283
MsG0180000565.01.T01 MTR_4g131580 56.371 259 106 3 5 263 17 268 6.64e-89 277
MsG0180000565.01.T01 MTR_8g079940 56.863 255 106 2 3 256 23 274 1.61e-88 274
MsG0180000565.01.T01 MTR_4g131600 56.589 258 104 4 1 257 16 266 2.31e-88 275
MsG0180000565.01.T01 MTR_1g013160 93.243 148 10 0 67 214 1 148 3.26e-86 255
MsG0180000565.01.T01 MTR_4g131570 52.985 268 124 2 5 271 17 283 3.64e-86 270
MsG0180000565.01.T01 MTR_3g086100 51.254 279 127 3 5 279 13 286 1.08e-84 265
MsG0180000565.01.T01 MTR_3g102590 51.292 271 75 4 2 269 5 221 4.73e-76 232
MsG0180000565.01.T01 MTR_3g102590 53.750 240 58 2 2 240 5 192 6.63e-71 218
MsG0180000565.01.T01 MTR_1g032570 53.000 200 89 2 64 259 1 199 4.46e-64 211
MsG0180000565.01.T01 MTR_3g102600 46.988 249 70 3 1 249 4 190 6.89e-60 191
MsG0180000565.01.T01 MTR_3g086100 48.636 220 104 3 64 279 1 215 2.23e-58 195
MsG0180000565.01.T01 MTR_4g021760 38.406 276 139 5 5 279 11 256 9.97e-57 185
MsG0180000565.01.T01 MTR_3g102600 46.058 241 68 3 2 242 5 183 9.97e-56 179
MsG0180000565.01.T01 MTR_4g021790 37.407 270 140 5 2 271 7 247 2.51e-50 167
MsG0180000565.01.T01 MTR_4g021855 37.652 247 126 3 2 248 7 225 2.92e-50 168
MsG0180000565.01.T01 MTR_4g021845 38.912 239 118 4 2 240 7 217 5.21e-49 162
MsG0180000565.01.T01 MTR_4g121020 44.554 202 79 4 84 271 6 188 1.88e-47 167
MsG0180000565.01.T01 MTR_0450s0040 37.344 241 130 3 9 249 8 227 7.16e-45 155
MsG0180000565.01.T01 MTR_8g105600 36.000 275 147 7 5 271 9 262 5.08e-40 147
MsG0180000565.01.T01 MTR_3g102600 41.872 203 56 3 47 249 1 141 1.31e-39 137
MsG0180000565.01.T01 MTR_6g045327 33.878 245 132 3 4 247 13 228 6.65e-38 138
MsG0180000565.01.T01 MTR_7g026400 34.855 241 127 3 32 265 14 231 1.88e-37 140
MsG0180000565.01.T01 MTR_3g102600 40.306 196 55 3 47 242 1 134 1.18e-35 125
MsG0180000565.01.T01 MTR_5g055260 33.603 247 109 7 2 248 7 198 2.34e-32 120
MsG0180000565.01.T01 MTR_8g032710 30.040 253 159 5 8 243 20 271 7.39e-29 115
MsG0180000565.01.T01 MTR_4g064730 47.788 113 53 2 156 262 94 206 2.94e-23 97.4
MsG0180000565.01.T01 MTR_3g092780 34.532 139 86 2 9 143 56 193 3.06e-23 100
MsG0180000565.01.T01 MTR_3g092780 34.532 139 86 2 9 143 56 193 3.56e-23 99.8
MsG0180000565.01.T01 MTR_6g016850 36.434 129 79 2 1 127 5 132 4.08e-23 94.0
MsG0180000565.01.T01 MTR_4g023980 32.530 166 85 3 52 217 36 174 1.07e-22 92.8
MsG0180000565.01.T01 MTR_8g024260 34.524 168 96 3 11 164 38 205 1.27e-22 98.2
MsG0180000565.01.T01 MTR_3g092780 37.008 127 75 2 9 131 56 181 3.15e-22 97.1
MsG0180000565.01.T01 MTR_3g092780 37.008 127 75 2 9 131 56 181 3.26e-22 97.1
MsG0180000565.01.T01 MTR_7g118260 35.484 155 85 4 2 144 5 156 1.81e-21 94.7
MsG0180000565.01.T01 MTR_7g118260 30.366 191 118 4 2 180 5 192 8.57e-21 92.8
MsG0180000565.01.T01 MTR_7g089010 53.846 78 36 0 172 249 72 149 2.84e-20 89.0
MsG0180000565.01.T01 MTR_1g067110 36.885 122 74 2 9 127 85 206 4.68e-20 90.9
MsG0180000565.01.T01 MTR_1g067110 36.885 122 74 2 9 127 85 206 5.28e-20 90.5
MsG0180000565.01.T01 MTR_4g061360 36.885 122 74 1 9 127 89 210 1.01e-19 89.7
MsG0180000565.01.T01 MTR_8g093040 29.098 244 130 4 4 247 13 213 1.18e-19 87.0
MsG0180000565.01.T01 MTR_6g453220 77.358 53 12 0 207 259 379 431 1.91e-19 88.6
MsG0180000565.01.T01 MTR_3g037390 33.871 124 79 1 9 129 24 147 1.10e-18 86.7
MsG0180000565.01.T01 MTR_5g014040 52.703 74 34 1 184 256 304 377 2.87e-17 82.4
MsG0180000565.01.T01 MTR_5g014040 35.200 125 75 3 5 127 14 134 2.06e-12 67.8
MsG0180000565.01.T01 MTR_4g108880 32.258 124 81 1 9 129 33 156 3.95e-17 82.0
MsG0180000565.01.T01 MTR_4g098870 55.072 69 30 1 189 256 328 396 2.42e-16 79.7
MsG0180000565.01.T01 MTR_4g098870 32.258 124 80 2 5 127 34 154 4.36e-11 63.9
MsG0180000565.01.T01 MTR_4g098870 55.072 69 30 1 189 256 308 376 2.46e-16 79.3
MsG0180000565.01.T01 MTR_4g098870 32.258 124 80 2 5 127 14 134 4.32e-11 63.5
MsG0180000565.01.T01 MTR_4g098870 55.072 69 30 1 189 256 308 376 2.50e-16 79.3
MsG0180000565.01.T01 MTR_4g098870 32.258 124 80 2 5 127 14 134 6.03e-11 63.2
MsG0180000565.01.T01 MTR_4g098870 55.072 69 30 1 189 256 347 415 2.73e-16 79.3
MsG0180000565.01.T01 MTR_4g098870 32.258 124 80 2 5 127 53 173 5.51e-11 63.5
MsG0180000565.01.T01 MTR_4g098870 55.072 69 30 1 189 256 328 396 2.77e-16 79.3
MsG0180000565.01.T01 MTR_4g098870 32.258 124 80 2 5 127 34 154 5.86e-11 63.5
MsG0180000565.01.T01 MTR_5g014040 49.367 79 34 2 184 256 264 342 2.87e-15 76.3
MsG0180000565.01.T01 MTR_5g014040 49.367 79 34 2 184 256 304 382 3.16e-15 76.3
MsG0180000565.01.T01 MTR_5g014040 35.200 125 75 3 5 127 14 134 2.15e-12 67.8
MsG0180000565.01.T01 MTR_4g078810 55.357 56 25 0 188 243 155 210 3.35e-15 75.9
MsG0180000565.01.T01 MTR_2g090675 55.357 56 25 0 188 243 158 213 8.07e-15 74.7
MsG0180000565.01.T01 MTR_4g086835 50.746 67 33 0 186 252 269 335 8.64e-15 74.7
MsG0180000565.01.T01 MTR_1g093080 41.379 87 50 1 177 262 197 283 4.08e-14 72.4
MsG0180000565.01.T01 MTR_4g113140 52.857 70 31 2 182 250 227 295 6.31e-14 72.0
MsG0180000565.01.T01 MTR_5g017980 51.613 62 30 0 188 249 190 251 9.49e-14 70.9
MsG0180000565.01.T01 MTR_5g017980 51.613 62 30 0 188 249 189 250 9.79e-14 70.9
MsG0180000565.01.T01 MTR_7g115530 64.286 56 19 1 189 243 216 271 1.11e-13 70.5
MsG0180000565.01.T01 MTR_7g115530 64.286 56 19 1 189 243 216 271 1.71e-13 70.1
MsG0180000565.01.T01 MTR_7g115530 54.286 70 31 1 189 257 216 285 1.81e-13 70.5
MsG0180000565.01.T01 MTR_7g115530 64.286 56 19 1 189 243 216 271 2.67e-13 70.1
MsG0180000565.01.T01 MTR_1g080330 60.345 58 22 1 187 243 171 228 2.67e-13 69.3
MsG0180000565.01.T01 MTR_4g086835 50.000 68 33 1 186 252 269 336 3.01e-13 70.1
MsG0180000565.01.T01 MTR_1g080330 60.345 58 22 1 187 243 171 228 3.57e-13 69.7
MsG0180000565.01.T01 MTR_2g023580 47.826 92 40 4 190 274 54 144 7.62e-13 67.4
MsG0180000565.01.T01 MTR_7g098250 52.941 68 31 1 187 253 258 325 8.30e-13 68.9
MsG0180000565.01.T01 MTR_5g054300 43.590 78 43 1 172 248 179 256 1.63e-12 67.4
MsG0180000565.01.T01 MTR_5g017980 50.794 63 30 1 188 249 190 252 2.10e-12 67.0
MsG0180000565.01.T01 MTR_1g090670 55.932 59 25 1 187 244 40 98 2.80e-12 66.2
MsG0180000565.01.T01 MTR_3g093860 35.385 130 63 5 11 122 20 146 4.24e-12 63.5
MsG0180000565.01.T01 MTR_6g088950 30.645 124 85 1 7 129 5 128 4.81e-12 64.3
MsG0180000565.01.T01 MTR_7g115530 62.963 54 19 1 189 241 216 269 6.98e-12 65.1
MsG0180000565.01.T01 MTR_2g086450 54.545 55 24 1 191 244 45 99 7.51e-12 65.9
MsG0180000565.01.T01 MTR_6g032990 54.545 55 24 1 191 244 42 96 7.61e-12 65.9
MsG0180000565.01.T01 MTR_7g490310 30.216 139 76 3 11 128 11 149 1.05e-11 63.9
MsG0180000565.01.T01 MTR_5g027440 56.364 55 23 1 191 244 25 79 1.50e-11 63.9
MsG0180000565.01.T01 MTR_8g038620 28.986 138 78 3 11 128 11 148 1.63e-11 62.4
MsG0180000565.01.T01 MTR_5g041350 50.909 55 26 1 191 244 15 69 5.23e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000565.01.T01 AT2G25180 63.953 258 87 3 3 259 11 263 2.25e-106 322
MsG0180000565.01.T01 AT4G31920 58.803 284 95 5 3 285 11 273 1.82e-102 310
MsG0180000565.01.T01 AT3G16857 49.477 287 127 2 3 271 31 317 5.70e-91 284
MsG0180000565.01.T01 AT3G16857 49.477 287 127 2 3 271 31 317 1.01e-90 284
MsG0180000565.01.T01 AT5G58080 55.396 278 98 5 3 271 12 272 7.35e-84 265
MsG0180000565.01.T01 AT1G67710 54.150 253 109 3 6 254 8 257 4.29e-80 252
MsG0180000565.01.T01 AT2G01760 47.138 297 138 5 1 282 3 295 4.51e-79 245
MsG0180000565.01.T01 AT3G16857 48.780 246 108 2 44 271 2 247 8.35e-74 238
MsG0180000565.01.T01 AT2G01760 43.077 325 138 6 1 282 3 323 4.83e-73 230
MsG0180000565.01.T01 AT1G67710 52.308 195 86 3 64 254 1 192 1.03e-53 181
MsG0180000565.01.T01 AT5G49240 38.819 237 142 2 5 240 38 272 1.08e-50 169
MsG0180000565.01.T01 AT2G01760 44.017 234 112 5 64 282 1 230 3.45e-50 169
MsG0180000565.01.T01 AT3G62670 34.628 309 159 9 10 292 42 333 1.17e-46 162
MsG0180000565.01.T01 AT1G67710 46.857 175 86 3 84 254 12 183 7.99e-39 142
MsG0180000565.01.T01 AT1G49190 31.206 282 175 4 4 274 29 302 2.05e-35 132
MsG0180000565.01.T01 AT5G07210 31.047 277 149 7 7 250 14 281 3.67e-33 128
MsG0180000565.01.T01 AT5G07210 30.175 285 149 8 7 250 14 289 1.44e-30 121
MsG0180000565.01.T01 AT2G27070 29.577 284 145 7 7 249 14 283 2.53e-28 114
MsG0180000565.01.T01 AT2G46790 39.837 123 69 2 9 127 37 158 5.33e-25 104
MsG0180000565.01.T01 AT2G46790 39.837 123 69 2 9 127 37 158 5.39e-25 104
MsG0180000565.01.T01 AT5G59570 48.214 112 50 2 170 278 120 226 9.81e-24 98.6
MsG0180000565.01.T01 AT5G59570 48.214 112 50 2 170 278 120 226 9.81e-24 98.6
MsG0180000565.01.T01 AT1G49190 37.600 125 78 0 4 128 29 153 1.60e-23 100
MsG0180000565.01.T01 AT2G40970 41.525 118 69 0 167 284 81 198 1.76e-23 97.1
MsG0180000565.01.T01 AT5G24470 36.719 128 76 2 7 130 48 174 2.26e-22 97.4
MsG0180000565.01.T01 AT3G46640 36.420 162 83 4 104 249 45 202 4.36e-22 94.7
MsG0180000565.01.T01 AT3G46640 37.107 159 86 4 104 249 45 202 4.53e-22 94.4
MsG0180000565.01.T01 AT3G46640 37.107 159 86 4 104 249 45 202 4.53e-22 94.4
MsG0180000565.01.T01 AT5G02810 38.525 122 72 1 9 127 78 199 2.80e-21 94.4
MsG0180000565.01.T01 AT5G05090 53.165 79 37 0 171 249 61 139 9.47e-21 89.7
MsG0180000565.01.T01 AT1G68210 25.632 277 177 3 9 257 13 288 1.23e-20 92.4
MsG0180000565.01.T01 AT5G60100 37.795 127 75 2 5 127 59 185 1.37e-20 91.3
MsG0180000565.01.T01 AT5G60100 37.795 127 75 2 5 127 59 185 1.37e-20 91.3
MsG0180000565.01.T01 AT5G60100 37.795 127 75 2 5 127 59 185 1.37e-20 91.3
MsG0180000565.01.T01 AT5G60100 37.795 127 75 2 5 127 59 185 1.47e-20 92.0
MsG0180000565.01.T01 AT5G60100 37.795 127 75 2 5 127 59 185 1.47e-20 92.0
MsG0180000565.01.T01 AT5G60100 37.795 127 75 2 5 127 59 185 1.47e-20 92.0
MsG0180000565.01.T01 AT5G60100 37.795 127 75 2 5 127 59 185 2.40e-20 91.3
MsG0180000565.01.T01 AT3G10760 65.000 60 21 0 190 249 104 163 2.44e-20 89.7
MsG0180000565.01.T01 AT5G44190 40.984 122 64 3 183 298 139 258 6.96e-19 86.3
MsG0180000565.01.T01 AT5G61380 33.871 124 79 1 9 129 19 142 7.86e-19 87.0
MsG0180000565.01.T01 AT5G44190 40.984 122 64 3 183 298 139 258 8.09e-19 85.9
MsG0180000565.01.T01 AT2G20570 44.330 97 43 1 153 249 126 211 1.91e-16 79.3
MsG0180000565.01.T01 AT2G20570 61.111 54 21 0 196 249 174 227 4.42e-15 75.5
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.25e-14 74.3
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.25e-14 74.3
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.25e-14 74.3
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.25e-14 74.3
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.25e-14 74.3
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.25e-14 74.3
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.40e-14 74.3
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.40e-14 74.3
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.40e-14 74.3
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.40e-14 74.3
MsG0180000565.01.T01 AT4G18020 55.932 59 26 0 196 254 301 359 1.40e-14 74.3
MsG0180000565.01.T01 AT2G03500 53.125 64 29 1 188 250 230 293 2.90e-13 70.1
MsG0180000565.01.T01 AT3G57040 28.395 162 94 2 11 150 11 172 9.28e-13 67.0
MsG0180000565.01.T01 AT3G57040 28.395 162 94 2 11 150 11 172 9.28e-13 67.0
MsG0180000565.01.T01 AT5G45580 43.182 88 47 2 181 265 11 98 3.96e-12 65.9
MsG0180000565.01.T01 AT3G04030 54.545 55 24 1 191 244 45 99 6.89e-12 65.9
MsG0180000565.01.T01 AT3G04030 54.545 55 24 1 191 244 45 99 7.55e-12 65.9
MsG0180000565.01.T01 AT3G56380 31.298 131 76 2 9 125 20 150 7.96e-12 62.8
MsG0180000565.01.T01 AT5G45580 44.737 76 41 1 191 265 7 82 1.07e-11 64.3
MsG0180000565.01.T01 AT3G04030 51.852 54 26 0 191 244 45 98 1.10e-11 65.1
MsG0180000565.01.T01 AT1G25550 51.667 60 28 1 190 248 208 267 1.25e-11 64.7
MsG0180000565.01.T01 AT3G56380 31.538 130 76 2 9 125 20 149 1.58e-11 62.0
MsG0180000565.01.T01 AT4G13640 39.048 105 54 3 187 281 33 137 2.12e-11 63.9
MsG0180000565.01.T01 AT4G13640 39.048 105 54 3 187 281 33 137 2.17e-11 63.9
MsG0180000565.01.T01 AT1G13300 47.692 65 33 1 185 248 175 239 2.45e-11 63.9
MsG0180000565.01.T01 AT3G24120 51.613 62 29 1 187 247 37 98 2.52e-11 63.5
MsG0180000565.01.T01 AT3G24120 51.613 62 29 1 187 247 37 98 2.58e-11 63.5
MsG0180000565.01.T01 AT2G40670 32.331 133 74 2 9 125 29 161 2.73e-11 61.6
MsG0180000565.01.T01 AT1G49560 45.833 72 38 1 186 256 187 258 4.42e-11 63.2
MsG0180000565.01.T01 AT4G37180 49.180 61 30 1 189 248 208 268 5.19e-11 63.2
MsG0180000565.01.T01 AT2G40670 32.576 132 74 2 9 125 29 160 5.82e-11 60.5
MsG0180000565.01.T01 AT4G37180 49.180 61 30 1 189 248 215 275 6.83e-11 62.8

Find 60 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAGAACATGTCTCAAGATTT+TGG 0.179210 1:-7938050 MsG0180000565.01.T01:CDS
ATTCCTTTCATCACCATCTT+TGG 0.256348 1:+7937210 None:intergenic
CCATTTCCACTATCAGAATT+AGG 0.318838 1:+7937072 None:intergenic
ATATGTGAGACTGAGAAGTA+TGG 0.327860 1:+7936197 None:intergenic
ATGAATGATCAGTTTCCTAT+TGG 0.330551 1:-7938100 MsG0180000565.01.T01:CDS
CCTAATTCTGATAGTGGAAA+TGG 0.330629 1:-7937072 MsG0180000565.01.T01:CDS
TTTAAGCTTCTTGAGCTTGT+TGG 0.375182 1:-7937365 MsG0180000565.01.T01:CDS
AAAGGTGGCCAGCCATCTTC+AGG 0.376644 1:-7936582 MsG0180000565.01.T01:intron
ACATGGTGCGTGTGATTATC+TGG 0.387305 1:-7937184 MsG0180000565.01.T01:CDS
TAAAAGAGGTACAGATCATT+TGG 0.388547 1:-7937146 MsG0180000565.01.T01:CDS
TATGAACATTGCTGATTACT+AGG 0.423694 1:+7937405 None:intergenic
TACCATGGTATTGACACCTT+TGG 0.431203 1:+7938017 None:intergenic
AGAATGTCACTGGGAAAGAT+TGG 0.434197 1:+7936231 None:intergenic
TCATTTGGCAGCATGTAATC+AGG 0.452747 1:-7937131 MsG0180000565.01.T01:CDS
GACGCGCCTAATTCTGATAG+TGG 0.455329 1:-7937078 MsG0180000565.01.T01:CDS
AGAAGAAGCCTCGAACTGTT+TGG 0.455763 1:-7936921 MsG0180000565.01.T01:CDS
TGAGCTTGTTGGACTTGAGA+TGG 0.457732 1:-7937354 MsG0180000565.01.T01:CDS
GCAAGAACTCGCATTCCAAT+AGG 0.458411 1:+7938085 None:intergenic
AGCTTAAATCCATCCATGTC+TGG 0.471845 1:+7937380 None:intergenic
GTTGTTGCGGTCAATCAATT+AGG 0.476132 1:-7936874 MsG0180000565.01.T01:CDS
GGGAAAGATTCGGCTGGGAC+AGG 0.479650 1:-7937051 MsG0180000565.01.T01:CDS
CTAATTCTGATAGTGGAAAT+GGG 0.482228 1:-7937071 MsG0180000565.01.T01:CDS
TGAAAATCTGACAAGGGAAA+AGG 0.489796 1:-7936600 MsG0180000565.01.T01:CDS
TAGCTAAGGCTGCTTCCATA+TGG 0.490232 1:+7936285 None:intergenic
CACTGCTCAAAACGCGATAA+CGG 0.499559 1:-7937465 MsG0180000565.01.T01:CDS
TAGTGGAAATGGGAAAGATT+CGG 0.502507 1:-7937061 MsG0180000565.01.T01:CDS
GTCAATCAATTAGGCATTGA+CGG 0.502566 1:-7936865 MsG0180000565.01.T01:intron
GGGTGGGAAAAGAATGTCAC+TGG 0.506615 1:+7936221 None:intergenic
AGCATAAAAGTACCTGAAGA+TGG 0.509866 1:+7936570 None:intergenic
ACATCGCAAGTTTGTTGTTG+CGG 0.516543 1:-7936887 MsG0180000565.01.T01:CDS
TATGTGAGACTGAGAAGTAT+GGG 0.518755 1:+7936198 None:intergenic
GAAACCTGTTAGACTAAAAG+AGG 0.521944 1:-7937160 MsG0180000565.01.T01:CDS
TGACAGTGAGGAAGAAAATG+AGG 0.529313 1:-7936974 MsG0180000565.01.T01:CDS
GGAAATGGGAAAGATTCGGC+TGG 0.530128 1:-7937057 MsG0180000565.01.T01:CDS
TGCATCTGAACTACTAGCTA+AGG 0.539318 1:+7936271 None:intergenic
TATTGGAATGCGAGTTCTTG+CGG 0.540178 1:-7938083 MsG0180000565.01.T01:CDS
TTGTATAACTTACTTATGAC+AGG 0.544810 1:+7937326 None:intergenic
TAAAAGTACCTGAAGATGGC+TGG 0.546379 1:+7936574 None:intergenic
TCAGTGGACCAAACAGTTCG+AGG 0.556377 1:+7936913 None:intergenic
CAATGTTCATATGCCAGACA+TGG 0.556391 1:-7937393 MsG0180000565.01.T01:CDS
AGATTGGCTGCTGAGTTGAT+AGG 0.561182 1:+7936247 None:intergenic
TAAGAATGAAGATGACAGTG+AGG 0.570920 1:-7936986 MsG0180000565.01.T01:CDS
GGAAGACAGACAAGACCATA+TGG 0.582020 1:-7936300 MsG0180000565.01.T01:CDS
GTTCATATGCCAGACATGGA+TGG 0.584384 1:-7937389 MsG0180000565.01.T01:CDS
ATGTGAGACTGAGAAGTATG+GGG 0.595182 1:+7936199 None:intergenic
TGCGAATGATGATCCAAAGA+TGG 0.615837 1:-7937223 MsG0180000565.01.T01:CDS
GGTCTTGTCTGTCTTCCGCA+CGG 0.622559 1:+7936306 None:intergenic
GAAATGGGAAAGATTCGGCT+GGG 0.627002 1:-7937056 MsG0180000565.01.T01:CDS
AAATCTGACAAGGGAAAAGG+TGG 0.629315 1:-7936597 MsG0180000565.01.T01:CDS
GATCCAAAGATGGTGATGAA+AGG 0.637771 1:-7937213 MsG0180000565.01.T01:CDS
ATAACTTACTTATGACAGGT+AGG 0.639228 1:+7937330 None:intergenic
TGTGAGACTGAGAAGTATGG+GGG 0.643481 1:+7936200 None:intergenic
CTCCAAAGGTGTCAATACCA+TGG 0.650196 1:-7938019 MsG0180000565.01.T01:intron
AAACTTGCGATGTAAATCAG+TGG 0.662856 1:+7936897 None:intergenic
GTGAGACTGAGAAGTATGGG+GGG 0.686210 1:+7936201 None:intergenic
GAATGTTGAAAATCTGACAA+GGG 0.692411 1:-7936606 MsG0180000565.01.T01:CDS
TGAATGTTGAAAATCTGACA+AGG 0.698854 1:-7936607 MsG0180000565.01.T01:CDS
GGTGGGAAAAGAATGTCACT+GGG 0.701317 1:+7936222 None:intergenic
GTGATGAAAGGAATTGAACA+TGG 0.742148 1:-7937201 MsG0180000565.01.T01:CDS
TCTACAAAGAATTAGCCGTG+CGG 0.798557 1:-7936321 MsG0180000565.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTTATTTGATATTGTTT+AGG - Chr1:7936547-7936566 MsG0180000565.01.T01:intron 10.0%
!!! AATGATATTAATTTTAGTTA+TGG - Chr1:7936771-7936790 MsG0180000565.01.T01:intron 10.0%
!!! TATTTTGAAATATTAAAGAT+TGG - Chr1:7936320-7936339 MsG0180000565.01.T01:CDS 10.0%
!! AAAAAACAAGATTAAAATCA+AGG + Chr1:7936347-7936366 None:intergenic 15.0%
!!! ATTATTATTTTCAATGAAGT+TGG - Chr1:7936441-7936460 MsG0180000565.01.T01:intron 15.0%
!! AAATCTAATGATATATAGCT+AGG - Chr1:7936375-7936394 MsG0180000565.01.T01:intron 20.0%
!! AATCTAATGATATATAGCTA+GGG - Chr1:7936376-7936395 MsG0180000565.01.T01:intron 20.0%
!! TATCGATAATATAAGATTGA+AGG + Chr1:7936983-7937002 None:intergenic 20.0%
! ATGAAAAACCATCTAGAAAA+AGG - Chr1:7937267-7937286 MsG0180000565.01.T01:intron 25.0%
! CATGATATTAATCTCAACAT+TGG + Chr1:7937483-7937502 None:intergenic 25.0%
! TTGTATAACTTACTTATGAC+AGG + Chr1:7936958-7936977 None:intergenic 25.0%
!! GCTAAACATAAGTATTTTTC+GGG + Chr1:7937561-7937580 None:intergenic 25.0%
!! GGCTAAACATAAGTATTTTT+CGG + Chr1:7937562-7937581 None:intergenic 25.0%
!! TAATTGATTTCTACAAGCAT+TGG + Chr1:7936517-7936536 None:intergenic 25.0%
!!! ATCAGTTCAAGAATCTTTTT+TGG + Chr1:7937655-7937674 None:intergenic 25.0%
AAAACCATCTAGAAAAAGGA+AGG - Chr1:7937271-7937290 MsG0180000565.01.T01:intron 30.0%
AATCGGAAAAATTCATAGCA+TGG + Chr1:7937583-7937602 None:intergenic 30.0%
ATAACTTACTTATGACAGGT+AGG + Chr1:7936954-7936973 None:intergenic 30.0%
ATGAATGATCAGTTTCCTAT+TGG - Chr1:7936181-7936200 MsG0180000565.01.T01:CDS 30.0%
GAATGTTGAAAATCTGACAA+GGG - Chr1:7937675-7937694 MsG0180000565.01.T01:intron 30.0%
TGAATGTTGAAAATCTGACA+AGG - Chr1:7937674-7937693 MsG0180000565.01.T01:intron 30.0%
TGAGAAAAACACTTCAGAAT+CGG + Chr1:7937600-7937619 None:intergenic 30.0%
TTCTCGATTCTTTCAAAGAT+TGG - Chr1:7936599-7936618 MsG0180000565.01.T01:CDS 30.0%
! ATGATATATAGCTAGGGTTT+TGG - Chr1:7936382-7936401 MsG0180000565.01.T01:intron 30.0%
! TAAAAGAGGTACAGATCATT+TGG - Chr1:7937135-7937154 MsG0180000565.01.T01:CDS 30.0%
! TAGAACATGTCTCAAGATTT+TGG - Chr1:7936231-7936250 MsG0180000565.01.T01:CDS 30.0%
! TATGAACATTGCTGATTACT+AGG + Chr1:7936879-7936898 None:intergenic 30.0%
! TTATCCTTCCTTTTTCTAGA+TGG + Chr1:7937278-7937297 None:intergenic 30.0%
!! CTAATTCTGATAGTGGAAAT+GGG - Chr1:7937210-7937229 MsG0180000565.01.T01:CDS 30.0%
!!! TTTTTCGGGAAGAATATTTG+AGG + Chr1:7937547-7937566 None:intergenic 30.0%
AAACTTGCGATGTAAATCAG+TGG + Chr1:7937387-7937406 None:intergenic 35.0%
ACGTAAGATCACCAAAGATT+AGG + Chr1:7936673-7936692 None:intergenic 35.0%
AGTTGTTATCTTACTTGCAG+AGG - Chr1:7937624-7937643 MsG0180000565.01.T01:intron 35.0%
ATATGTGAGACTGAGAAGTA+TGG + Chr1:7938087-7938106 None:intergenic 35.0%
ATTCCTTTCATCACCATCTT+TGG + Chr1:7937074-7937093 None:intergenic 35.0%
CCATTTCCACTATCAGAATT+AGG + Chr1:7937212-7937231 None:intergenic 35.0%
GAAACCTGTTAGACTAAAAG+AGG - Chr1:7937121-7937140 MsG0180000565.01.T01:CDS 35.0%
GTGATGAAAGGAATTGAACA+TGG - Chr1:7937080-7937099 MsG0180000565.01.T01:CDS 35.0%
TAAGAATGAAGATGACAGTG+AGG - Chr1:7937295-7937314 MsG0180000565.01.T01:intron 35.0%
TAGTGGAAATGGGAAAGATT+CGG - Chr1:7937220-7937239 MsG0180000565.01.T01:CDS 35.0%
TGAAAATCTGACAAGGGAAA+AGG - Chr1:7937681-7937700 MsG0180000565.01.T01:intron 35.0%
TTTAAGCTTCTTGAGCTTGT+TGG - Chr1:7936916-7936935 MsG0180000565.01.T01:CDS 35.0%
! TATGTGAGACTGAGAAGTAT+GGG + Chr1:7938086-7938105 None:intergenic 35.0%
! TCTTTTGTGTGCCTAATCTT+TGG - Chr1:7936659-7936678 MsG0180000565.01.T01:intron 35.0%
! TGTACCTCTTTTAGTCTAAC+AGG + Chr1:7937128-7937147 None:intergenic 35.0%
!! AGCATAAAAGTACCTGAAGA+TGG + Chr1:7937714-7937733 None:intergenic 35.0%
!! CCTAATTCTGATAGTGGAAA+TGG - Chr1:7937209-7937228 MsG0180000565.01.T01:CDS 35.0%
!! GTCAATCAATTAGGCATTGA+CGG - Chr1:7937416-7937435 MsG0180000565.01.T01:CDS 35.0%
!! TTTTGGAGAAACTTCTCCAA+AGG - Chr1:7936248-7936267 MsG0180000565.01.T01:CDS 35.0%
AAATCTGACAAGGGAAAAGG+TGG - Chr1:7937684-7937703 MsG0180000565.01.T01:intron 40.0%
AATCTGCGCATTGAAGAAAG+AGG + Chr1:7937452-7937471 None:intergenic 40.0%
AGAATGTCACTGGGAAAGAT+TGG + Chr1:7938053-7938072 None:intergenic 40.0%
AGCTTAAATCCATCCATGTC+TGG + Chr1:7936904-7936923 None:intergenic 40.0%
ATCTGCGCATTGAAGAAAGA+GGG + Chr1:7937451-7937470 None:intergenic 40.0%
ATTAACAGTGTCCTTGTGTC+AGG + Chr1:7937784-7937803 None:intergenic 40.0%
CAATGTTCATATGCCAGACA+TGG - Chr1:7936888-7936907 MsG0180000565.01.T01:CDS 40.0%
GTTGTTGCGGTCAATCAATT+AGG - Chr1:7937407-7937426 MsG0180000565.01.T01:CDS 40.0%
TATTGGAATGCGAGTTCTTG+CGG - Chr1:7936198-7936217 MsG0180000565.01.T01:CDS 40.0%
TCATTTGGCAGCATGTAATC+AGG - Chr1:7937150-7937169 MsG0180000565.01.T01:CDS 40.0%
TCTACAAAGAATTAGCCGTG+CGG - Chr1:7937960-7937979 MsG0180000565.01.T01:intron 40.0%
TGACACAAGGACACTGTTAA+TGG - Chr1:7937783-7937802 MsG0180000565.01.T01:intron 40.0%
TGACAGTGAGGAAGAAAATG+AGG - Chr1:7937307-7937326 MsG0180000565.01.T01:intron 40.0%
TGCATCTGAACTACTAGCTA+AGG + Chr1:7938013-7938032 None:intergenic 40.0%
TGCGAATGATGATCCAAAGA+TGG - Chr1:7937058-7937077 MsG0180000565.01.T01:CDS 40.0%
TGTTGATGTCGTGTTAGTGT+CGG - Chr1:7937841-7937860 MsG0180000565.01.T01:intron 40.0%
! ATGTGAGACTGAGAAGTATG+GGG + Chr1:7938085-7938104 None:intergenic 40.0%
! GATCCAAAGATGGTGATGAA+AGG - Chr1:7937068-7937087 MsG0180000565.01.T01:CDS 40.0%
!! ACATCGCAAGTTTGTTGTTG+CGG - Chr1:7937394-7937413 MsG0180000565.01.T01:CDS 40.0%
!! GGTGATCTTACGTTGTTTCT+TGG - Chr1:7936680-7936699 MsG0180000565.01.T01:intron 40.0%
!! GTTGATGATGAAGCATACCA+TGG + Chr1:7936282-7936301 None:intergenic 40.0%
!! TAAAAGTACCTGAAGATGGC+TGG + Chr1:7937710-7937729 None:intergenic 40.0%
!! TACCATGGTATTGACACCTT+TGG + Chr1:7936267-7936286 None:intergenic 40.0%
!!! CTAGGGTTTTGGCTAAGTTT+TGG - Chr1:7936393-7936412 MsG0180000565.01.T01:intron 40.0%
ACATGGTGCGTGTGATTATC+TGG - Chr1:7937097-7937116 MsG0180000565.01.T01:CDS 45.0%
AGAAGAAGCCTCGAACTGTT+TGG - Chr1:7937360-7937379 MsG0180000565.01.T01:CDS 45.0%
CACTGCTCAAAACGCGATAA+CGG - Chr1:7936816-7936835 MsG0180000565.01.T01:intron 45.0%
CCTATAATGAGCCTGACACA+AGG - Chr1:7937770-7937789 MsG0180000565.01.T01:intron 45.0%
CCTTGTGTCAGGCTCATTAT+AGG + Chr1:7937773-7937792 None:intergenic 45.0%
GAAATGGGAAAGATTCGGCT+GGG - Chr1:7937225-7937244 MsG0180000565.01.T01:CDS 45.0%
GCAAGAACTCGCATTCCAAT+AGG + Chr1:7936199-7936218 None:intergenic 45.0%
GGAAGACAGACAAGACCATA+TGG - Chr1:7937981-7938000 MsG0180000565.01.T01:intron 45.0%
GGTGGGAAAAGAATGTCACT+GGG + Chr1:7938062-7938081 None:intergenic 45.0%
GTTCATATGCCAGACATGGA+TGG - Chr1:7936892-7936911 MsG0180000565.01.T01:CDS 45.0%
TAGCTAAGGCTGCTTCCATA+TGG + Chr1:7937999-7938018 None:intergenic 45.0%
! TGTGAGACTGAGAAGTATGG+GGG + Chr1:7938084-7938103 None:intergenic 45.0%
!! AGATTGGCTGCTGAGTTGAT+AGG + Chr1:7938037-7938056 None:intergenic 45.0%
!! CTCCAAAGGTGTCAATACCA+TGG - Chr1:7936262-7936281 MsG0180000565.01.T01:CDS 45.0%
!! TGAGCTTGTTGGACTTGAGA+TGG - Chr1:7936927-7936946 MsG0180000565.01.T01:CDS 45.0%
GACGCGCCTAATTCTGATAG+TGG - Chr1:7937203-7937222 MsG0180000565.01.T01:CDS 50.0%
GGAAATGGGAAAGATTCGGC+TGG - Chr1:7937224-7937243 MsG0180000565.01.T01:CDS 50.0%
GGGTGGGAAAAGAATGTCAC+TGG + Chr1:7938063-7938082 None:intergenic 50.0%
TCAGTGGACCAAACAGTTCG+AGG + Chr1:7937371-7937390 None:intergenic 50.0%
! GTGAGACTGAGAAGTATGGG+GGG + Chr1:7938083-7938102 None:intergenic 50.0%
!! CGTTATCGCGTTTTGAGCAG+TGG + Chr1:7936818-7936837 None:intergenic 50.0%
AAAGGTGGCCAGCCATCTTC+AGG - Chr1:7937699-7937718 MsG0180000565.01.T01:intron 55.0%
! AGACTGAGAAGTATGGGGGG+TGG + Chr1:7938080-7938099 None:intergenic 55.0%
! GACTGAGAAGTATGGGGGGT+GGG + Chr1:7938079-7938098 None:intergenic 55.0%
! GGTCTTGTCTGTCTTCCGCA+CGG + Chr1:7937978-7937997 None:intergenic 55.0%
GGACGCAGAATGCACAGCGT+CGG - Chr1:7937862-7937881 MsG0180000565.01.T01:intron 60.0%
GGGAAAGATTCGGCTGGGAC+AGG - Chr1:7937230-7937249 MsG0180000565.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 7936181 7938122 7936181 ID=MsG0180000565.01;Name=MsG0180000565.01
Chr1 mRNA 7936181 7938122 7936181 ID=MsG0180000565.01.T01;Parent=MsG0180000565.01;Name=MsG0180000565.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|5|0|298
Chr1 exon 7938020 7938122 7938020 ID=MsG0180000565.01.T01:exon:5878;Parent=MsG0180000565.01.T01
Chr1 exon 7937339 7937491 7937339 ID=MsG0180000565.01.T01:exon:5879;Parent=MsG0180000565.01.T01
Chr1 exon 7936866 7937252 7936866 ID=MsG0180000565.01.T01:exon:5880;Parent=MsG0180000565.01.T01
Chr1 exon 7936583 7936659 7936583 ID=MsG0180000565.01.T01:exon:5881;Parent=MsG0180000565.01.T01
Chr1 exon 7936181 7936357 7936181 ID=MsG0180000565.01.T01:exon:5882;Parent=MsG0180000565.01.T01
Chr1 CDS 7938020 7938122 7938020 ID=MsG0180000565.01.T01:cds;Parent=MsG0180000565.01.T01
Chr1 CDS 7937339 7937491 7937339 ID=MsG0180000565.01.T01:cds;Parent=MsG0180000565.01.T01
Chr1 CDS 7936866 7937252 7936866 ID=MsG0180000565.01.T01:cds;Parent=MsG0180000565.01.T01
Chr1 CDS 7936583 7936659 7936583 ID=MsG0180000565.01.T01:cds;Parent=MsG0180000565.01.T01
Chr1 CDS 7936181 7936357 7936181 ID=MsG0180000565.01.T01:cds;Parent=MsG0180000565.01.T01
Gene Sequence

>MsG0180000565.01.T01

ATGAATGATCAGTTTCCTATTGGAATGCGAGTTCTTGCGGTTGATGATGATAGAACATGTCTCAAGATTTTGGAGAAACTTCTCCAAAGGTGTCAATACCATGTAACCACTGCTCAAAACGCGATAACGGCGTTGAACTTGTTGAGAGAAAACAAAAGCAATTTTGACCTAGTAATCAGCAATGTTCATATGCCAGACATGGATGGATTTAAGCTTCTTGAGCTTGTTGGACTTGAGATGGACCTACCTGTCATAATGTTTTCTGCGAATGATGATCCAAAGATGGTGATGAAAGGAATTGAACATGGTGCGTGTGATTATCTGGTGAAACCTGTTAGACTAAAAGAGGTACAGATCATTTGGCAGCATGTAATCAGGAAGAAGAAAACTAGCAAACGAAGCAATCATGACGCGCCTAATTCTGATAGTGGAAATGGGAAAGATTCGGCTGGGACAGGAAATTCTGATAAGAATGAAAAACCATCTAGAAAAAGGAAGGATAAGAATGAAGATGACAGTGAGGAAGAAAATGAGGATGATTATGACAATGATGATCAAACTGCGCAGAAGAAGCCTCGAACTGTTTGGTCCACTGATTTACATCGCAAGTTTGTTGTTGCGGTCAATCAATTAGGCATTGACGAGGCTGTACCAAAAAAGATTCTTGAACTGATGAATGTTGAAAATCTGACAAGGGAAAAGGTGGCCAGCCATCTTCAGAAGTATAGACTCTATCTACAAAGAATTAGCCGTGCGGAAGACAGACAAGACCATATGGAAGCAGCCTTAGCTAGTAGTTCAGATGCATCCTATCAACTCAGCAGCCAATCTTTCCCAGTGACATTCTTTTCCCACCCCCCATACTTCTCAGTCTCACATATCTTTCGTGATTTGTAA
Protein sequence

>MsG0180000565.01.T01

MNDQFPIGMRVLAVDDDRTCLKILEKLLQRCQYHVTTAQNAITALNLLRENKSNFDLVISNVHMPDMDGFKLLELVGLEMDLPVIMFSANDDPKMVMKGIEHGACDYLVKPVRLKEVQIIWQHVIRKKKTSKRSNHDAPNSDSGNGKDSAGTGNSDKNEKPSRKRKDKNEDDSEEENEDDYDNDDQTAQKKPRTVWSTDLHRKFVVAVNQLGIDEAVPKKILELMNVENLTREKVASHLQKYRLYLQRISRAEDRQDHMEAALASSSDASYQLSSQSFPVTFFSHPPYFSVSHIFRDL*