AlfalfaGEDB | MsG0180000565.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000565.01

KEGG: -
GO: GO:0000160 GO:0003677 GO:0003700 GO:0007165
PFAM: PF00072 PF00249

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000565.01.T01	KEH39870.1	96.31	271	10	0	1	271	1	271	1.27E-166	482

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000565.01.T01	Q6H805	62.633	281	91	3	3	275	23	297	3.43E-118	355

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000565.01.T01	A0A072VP36	96.310	271	10	0	1	271	1	271	6.09e-167	482

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TFs/TRs:

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Gene ID	Type	Classification
MsG0180000565.01.T01	TF	GARP-ARR-B

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000565.01.T01	MTR_1g013170	96.310	271	10	0	1	271	1	271	1.54e-170	482
MsG0180000565.01.T01	MTR_7g117705	80.524	267	50	2	1	266	1	266	3.42e-142	409
MsG0180000565.01.T01	MTR_1g013180	80.443	271	46	1	1	271	152	415	5.86e-142	416
MsG0180000565.01.T01	MTR_3g106220	69.091	275	78	3	2	271	5	277	4.33e-123	366
MsG0180000565.01.T01	MTR_2g450070	58.696	276	102	3	3	269	16	288	1.51e-98	303
MsG0180000565.01.T01	MTR_2g450070	58.696	276	102	3	3	269	16	288	2.77e-98	303
MsG0180000565.01.T01	MTR_4g121020	53.169	284	100	4	2	271	28	292	2.13e-94	293
MsG0180000565.01.T01	MTR_4g121020	53.169	284	100	4	2	271	28	292	2.69e-94	293
MsG0180000565.01.T01	MTR_2g034960	49.825	285	119	2	1	275	25	295	9.94e-93	287
MsG0180000565.01.T01	MTR_1g032570	53.846	260	115	2	4	259	10	268	5.02e-91	283

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000565.01.T01	AT2G25180	63.953	258	87	3	3	259	11	263	2.25e-106	322
MsG0180000565.01.T01	AT4G31920	58.803	284	95	5	3	285	11	273	1.82e-102	310
MsG0180000565.01.T01	AT3G16857	49.477	287	127	2	3	271	31	317	5.70e-91	284
MsG0180000565.01.T01	AT3G16857	49.477	287	127	2	3	271	31	317	1.01e-90	284
MsG0180000565.01.T01	AT5G58080	55.396	278	98	5	3	271	12	272	7.35e-84	265
MsG0180000565.01.T01	AT1G67710	54.150	253	109	3	6	254	8	257	4.29e-80	252
MsG0180000565.01.T01	AT2G01760	47.138	297	138	5	1	282	3	295	4.51e-79	245
MsG0180000565.01.T01	AT3G16857	48.780	246	108	2	44	271	2	247	8.35e-74	238
MsG0180000565.01.T01	AT2G01760	43.077	325	138	6	1	282	3	323	4.83e-73	230
MsG0180000565.01.T01	AT1G67710	52.308	195	86	3	64	254	1	192	1.03e-53	181

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Find 60 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTGCGATGTAAATCAG+TGG	0.662856	1:+7936897	None:intergenic
AAAGGTGGCCAGCCATCTTC+AGG	0.376644	1:-7936582	MsG0180000565.01.T01:intron
AAATCTGACAAGGGAAAAGG+TGG	0.629315	1:-7936597	MsG0180000565.01.T01:CDS
ACATCGCAAGTTTGTTGTTG+CGG	0.516543	1:-7936887	MsG0180000565.01.T01:CDS
ACATGGTGCGTGTGATTATC+TGG	0.387305	1:-7937184	MsG0180000565.01.T01:CDS
AGAAGAAGCCTCGAACTGTT+TGG	0.455763	1:-7936921	MsG0180000565.01.T01:CDS
AGAATGTCACTGGGAAAGAT+TGG	0.434197	1:+7936231	None:intergenic
AGATTGGCTGCTGAGTTGAT+AGG	0.561182	1:+7936247	None:intergenic
AGCATAAAAGTACCTGAAGA+TGG	0.509866	1:+7936570	None:intergenic
AGCTTAAATCCATCCATGTC+TGG	0.471845	1:+7937380	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACCATCTAGAAAAAGGA+AGG	-	Chr1:7937271-7937290	MsG0180000565.01.T01:intron	30.0%
AATCGGAAAAATTCATAGCA+TGG	+	Chr1:7937583-7937602	None:intergenic	30.0%
ATAACTTACTTATGACAGGT+AGG	+	Chr1:7936954-7936973	None:intergenic	30.0%
ATGAATGATCAGTTTCCTAT+TGG	-	Chr1:7936181-7936200	MsG0180000565.01.T01:CDS	30.0%
GAATGTTGAAAATCTGACAA+GGG	-	Chr1:7937675-7937694	MsG0180000565.01.T01:intron	30.0%
TGAATGTTGAAAATCTGACA+AGG	-	Chr1:7937674-7937693	MsG0180000565.01.T01:intron	30.0%
TGAGAAAAACACTTCAGAAT+CGG	+	Chr1:7937600-7937619	None:intergenic	30.0%
TTCTCGATTCTTTCAAAGAT+TGG	-	Chr1:7936599-7936618	MsG0180000565.01.T01:CDS	30.0%
AAACTTGCGATGTAAATCAG+TGG	+	Chr1:7937387-7937406	None:intergenic	35.0%
ACGTAAGATCACCAAAGATT+AGG	+	Chr1:7936673-7936692	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	7936181	7938122	7936181	ID=MsG0180000565.01;Name=MsG0180000565.01
Chr1	mRNA	7936181	7938122	7936181	ID=MsG0180000565.01.T01;Parent=MsG0180000565.01;Name=MsG0180000565.01.T01;_AED=0.44;_eAED=0.44;_QI=0\|0\|0\|1\|1\|1\|5\|0\|298
Chr1	exon	7938020	7938122	7938020	ID=MsG0180000565.01.T01:exon:5878;Parent=MsG0180000565.01.T01
Chr1	exon	7937339	7937491	7937339	ID=MsG0180000565.01.T01:exon:5879;Parent=MsG0180000565.01.T01
Chr1	exon	7936866	7937252	7936866	ID=MsG0180000565.01.T01:exon:5880;Parent=MsG0180000565.01.T01
Chr1	exon	7936583	7936659	7936583	ID=MsG0180000565.01.T01:exon:5881;Parent=MsG0180000565.01.T01
Chr1	exon	7936181	7936357	7936181	ID=MsG0180000565.01.T01:exon:5882;Parent=MsG0180000565.01.T01
Chr1	CDS	7938020	7938122	7938020	ID=MsG0180000565.01.T01:cds;Parent=MsG0180000565.01.T01
Chr1	CDS	7937339	7937491	7937339	ID=MsG0180000565.01.T01:cds;Parent=MsG0180000565.01.T01
Chr1	CDS	7936866	7937252	7936866	ID=MsG0180000565.01.T01:cds;Parent=MsG0180000565.01.T01
Chr1	CDS	7936583	7936659	7936583	ID=MsG0180000565.01.T01:cds;Parent=MsG0180000565.01.T01
Chr1	CDS	7936181	7936357	7936181	ID=MsG0180000565.01.T01:cds;Parent=MsG0180000565.01.T01

Gene Sequence

>MsG0180000565.01.T01

ATGAATGATCAGTTTCCTATTGGAATGCGAGTTCTTGCGGTTGATGATGATAGAACATGTCTCAAGATTTTGGAGAAACTTCTCCAAAGGTGTCAATACCATGTAACCACTGCTCAAAACGCGATAACGGCGTTGAACTTGTTGAGAGAAAACAAAAGCAATTTTGACCTAGTAATCAGCAATGTTCATATGCCAGACATGGATGGATTTAAGCTTCTTGAGCTTGTTGGACTTGAGATGGACCTACCTGTCATAATGTTTTCTGCGAATGATGATCCAAAGATGGTGATGAAAGGAATTGAACATGGTGCGTGTGATTATCTGGTGAAACCTGTTAGACTAAAAGAGGTACAGATCATTTGGCAGCATGTAATCAGGAAGAAGAAAACTAGCAAACGAAGCAATCATGACGCGCCTAATTCTGATAGTGGAAATGGGAAAGATTCGGCTGGGACAGGAAATTCTGATAAGAATGAAAAACCATCTAGAAAAAGGAAGGATAAGAATGAAGATGACAGTGAGGAAGAAAATGAGGATGATTATGACAATGATGATCAAACTGCGCAGAAGAAGCCTCGAACTGTTTGGTCCACTGATTTACATCGCAAGTTTGTTGTTGCGGTCAATCAATTAGGCATTGACGAGGCTGTACCAAAAAAGATTCTTGAACTGATGAATGTTGAAAATCTGACAAGGGAAAAGGTGGCCAGCCATCTTCAGAAGTATAGACTCTATCTACAAAGAATTAGCCGTGCGGAAGACAGACAAGACCATATGGAAGCAGCCTTAGCTAGTAGTTCAGATGCATCCTATCAACTCAGCAGCCAATCTTTCCCAGTGACATTCTTTTCCCACCCCCCATACTTCTCAGTCTCACATATCTTTCGTGATTTGTAA

Protein sequence

>MsG0180000565.01.T01

MNDQFPIGMRVLAVDDDRTCLKILEKLLQRCQYHVTTAQNAITALNLLRENKSNFDLVISNVHMPDMDGFKLLELVGLEMDLPVIMFSANDDPKMVMKGIEHGACDYLVKPVRLKEVQIIWQHVIRKKKTSKRSNHDAPNSDSGNGKDSAGTGNSDKNEKPSRKRKDKNEDDSEEENEDDYDNDDQTAQKKPRTVWSTDLHRKFVVAVNQLGIDEAVPKKILELMNVENLTREKVASHLQKYRLYLQRISRAEDRQDHMEAALASSSDASYQLSSQSFPVTFFSHPPYFSVSHIFRDL*