Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000771.01.T01 | ABO33479.1 | 69.737 | 152 | 6 | 1 | 1 | 112 | 137 | 288 | 5.36E-63 | 203 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000771.01.T01 | O04135 | 64.474 | 152 | 13 | 2 | 1 | 112 | 247 | 397 | 8.52E-57 | 183 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000771.01.T01 | A5Y4G7 | 69.737 | 152 | 6 | 1 | 1 | 112 | 137 | 288 | 2.56e-63 | 203 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180000771.01.T01 | TF | HB-other |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000771.01.T01 | MTR_1g017080 | 69.737 | 152 | 6 | 1 | 1 | 112 | 228 | 379 | 4.26e-66 | 204 |
| MsG0180000771.01.T01 | MTR_2g024390 | 46.617 | 133 | 23 | 3 | 7 | 99 | 234 | 358 | 3.30e-25 | 97.4 |
| MsG0180000771.01.T01 | MTR_5g085860 | 80.435 | 46 | 9 | 0 | 43 | 88 | 231 | 276 | 3.59e-19 | 80.5 |
| MsG0180000771.01.T01 | MTR_5g033720 | 37.795 | 127 | 36 | 2 | 3 | 89 | 185 | 308 | 5.77e-19 | 80.1 |
| MsG0180000771.01.T01 | MTR_1g084060 | 60.377 | 53 | 21 | 0 | 43 | 95 | 213 | 265 | 5.67e-17 | 73.9 |
| MsG0180000771.01.T01 | MTR_1g084060 | 60.377 | 53 | 21 | 0 | 43 | 95 | 213 | 265 | 8.15e-17 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000771.01.T01 | AT4G08150 | 62.092 | 153 | 15 | 4 | 2 | 112 | 247 | 398 | 1.31e-52 | 170 |
| MsG0180000771.01.T01 | AT1G62360 | 40.690 | 145 | 34 | 3 | 5 | 105 | 229 | 365 | 4.56e-25 | 97.4 |
| MsG0180000771.01.T01 | AT1G23380 | 38.843 | 121 | 34 | 1 | 7 | 87 | 197 | 317 | 4.35e-19 | 80.5 |
| MsG0180000771.01.T01 | AT1G23380 | 38.843 | 121 | 34 | 1 | 7 | 87 | 199 | 319 | 5.01e-19 | 80.5 |
| MsG0180000771.01.T01 | AT1G70510 | 71.111 | 45 | 13 | 0 | 43 | 87 | 257 | 301 | 9.47e-17 | 73.9 |
| MsG0180000771.01.T01 | AT1G70510 | 71.111 | 45 | 13 | 0 | 43 | 87 | 261 | 305 | 9.87e-17 | 73.9 |
Find 30 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCCGAAGACATGCAGTTTA+TGG | 0.230587 | 1:-10878468 | MsG0180000771.01.T01:CDS |
| GCTGATGGTCCCTACCGTTT+AGG | 0.303999 | 1:-10878389 | MsG0180000771.01.T01:CDS |
| ACAGAGTGCAGCTCTTTATA+TGG | 0.322707 | 1:-10878426 | MsG0180000771.01.T01:CDS |
| CTGATGGTCCCTACCGTTTA+GGG | 0.368805 | 1:-10878388 | MsG0180000771.01.T01:CDS |
| CACCATAAACTGCATGTCTT+CGG | 0.375582 | 1:+10878466 | None:intergenic |
| ATCAGAAGCAAATAAATAAC+TGG | 0.399549 | 1:-10878523 | MsG0180000771.01.T01:CDS |
| ACCATAAACTGCATGTCTTC+GGG | 0.435756 | 1:+10878467 | None:intergenic |
| ATCATAATCATGGCCCTAAA+CGG | 0.455352 | 1:+10878375 | None:intergenic |
| GGTTCATAAATCAAAGGAAC+AGG | 0.472183 | 1:-10878502 | MsG0180000771.01.T01:CDS |
| TAAATCAAAGGAACAGGCAC+TGG | 0.485351 | 1:-10878496 | MsG0180000771.01.T01:CDS |
| GTAGCATTGGCTGAGTCTAC+AGG | 0.488800 | 1:-10878551 | MsG0180000771.01.T01:CDS |
| TCTGCTCGAGGATCAATCTC+TGG | 0.491728 | 1:+10879085 | None:intergenic |
| GAGGATCAAGAGAATAGTGG+TGG | 0.527205 | 1:-10879124 | MsG0180000771.01.T01:CDS |
| AATTCACGGTCTTCTGCTCG+AGG | 0.528363 | 1:+10879073 | None:intergenic |
| CTTGCTGAAGAAATATATAG+TGG | 0.531930 | 1:-10879041 | MsG0180000771.01.T01:intron |
| GAAGAGGATCAAGAGAATAG+TGG | 0.545053 | 1:-10879127 | MsG0180000771.01.T01:CDS |
| CTATATATTTCTTCAGCAAG+TGG | 0.545542 | 1:+10879044 | None:intergenic |
| GGAATCAGAGAAGGTAGCAT+TGG | 0.547951 | 1:-10878564 | MsG0180000771.01.T01:CDS |
| ATTGGCTGAGTCTACAGGAT+TGG | 0.556994 | 1:-10878546 | MsG0180000771.01.T01:CDS |
| TAATCATGGCCCTAAACGGT+AGG | 0.564867 | 1:+10878379 | None:intergenic |
| AGACATGCAGTTTATGGTGA+TGG | 0.566448 | 1:-10878462 | MsG0180000771.01.T01:CDS |
| AGTGCAGCTCTTTATATGGA+TGG | 0.581356 | 1:-10878422 | MsG0180000771.01.T01:CDS |
| ATAACTGGTTCATAAATCAA+AGG | 0.595397 | 1:-10878508 | MsG0180000771.01.T01:CDS |
| GATGGTCATTACATAGCTGA+TGG | 0.595725 | 1:-10878404 | MsG0180000771.01.T01:CDS |
| AGGCTCTGGTTCATCAGAAG+AGG | 0.608721 | 1:-10879143 | MsG0180000771.01.T01:CDS |
| AATCATGGCCCTAAACGGTA+GGG | 0.623950 | 1:+10878380 | None:intergenic |
| ATGCAGTTTATGGTGATGGA+TGG | 0.624056 | 1:-10878458 | MsG0180000771.01.T01:CDS |
| ATATAAAGAGCTGCACTCTG+TGG | 0.627158 | 1:+10878428 | None:intergenic |
| ATAAACTGCATGTCTTCGGG+TGG | 0.642266 | 1:+10878470 | None:intergenic |
| CACTATATAGGAATCAGAGA+AGG | 0.647456 | 1:-10878573 | MsG0180000771.01.T01:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | CAAATTAAAATACATATATT+TGG | - | Chr1:10878767-10878786 | MsG0180000771.01.T01:intron | 10.0% |
| !!! | ATTTTTTTTTTCTTTCTAAA+AGG | - | Chr1:10878917-10878936 | MsG0180000771.01.T01:intron | 10.0% |
| !!! | CAAATATATGTATTTTAATT+TGG | + | Chr1:10878769-10878788 | None:intergenic | 10.0% |
| !!! | TTTTTTTTTTCTTTCTAAAA+GGG | - | Chr1:10878918-10878937 | MsG0180000771.01.T01:intron | 10.0% |
| !! | GTATTAGTATTATATATAGA+CGG | + | Chr1:10878698-10878717 | None:intergenic | 15.0% |
| !!! | ATGTGATTTTTATTTTTGAT+TGG | - | Chr1:10878842-10878861 | MsG0180000771.01.T01:intron | 15.0% |
| !!! | TTTTAATTTGGATCAATTTT+TGG | + | Chr1:10878757-10878776 | None:intergenic | 15.0% |
| !! | TATATAGACGGTAAAATAAT+AGG | + | Chr1:10878686-10878705 | None:intergenic | 20.0% |
| !! | TCAAAAATAAAAATCACATG+TGG | + | Chr1:10878841-10878860 | None:intergenic | 20.0% |
| !! | TGTATATATGATCACTATAT+AGG | - | Chr1:10878951-10878970 | MsG0180000771.01.T01:intron | 20.0% |
| !!! | AAACATTACATGTTGTATTT+TGG | - | Chr1:10878727-10878746 | MsG0180000771.01.T01:intron | 20.0% |
| ! | ATCAGAAGCAAATAAATAAC+TGG | - | Chr1:10879013-10879032 | MsG0180000771.01.T01:intron | 25.0% |
| !! | ATAACTGGTTCATAAATCAA+AGG | - | Chr1:10879028-10879047 | MsG0180000771.01.T01:intron | 25.0% |
| !! | ATTTTCCTTTCTTTTCTTCT+TGG | + | Chr1:10878550-10878569 | None:intergenic | 25.0% |
| ACAAAAGCTACTTAATTGGT+GGG | - | Chr1:10878586-10878605 | MsG0180000771.01.T01:intron | 30.0% | |
| ACATATATTTGGATGATACG+AGG | - | Chr1:10878778-10878797 | MsG0180000771.01.T01:intron | 30.0% | |
| ACTTTCCAAGAAGAAAAGAA+AGG | - | Chr1:10878542-10878561 | MsG0180000771.01.T01:CDS | 30.0% | |
| CTATATATTTCTTCAGCAAG+TGG | + | Chr1:10878495-10878514 | None:intergenic | 30.0% | |
| CTTGCTGAAGAAATATATAG+TGG | - | Chr1:10878495-10878514 | MsG0180000771.01.T01:CDS | 30.0% | |
| GAAAGGAAAATTACCTAAAG+AGG | - | Chr1:10878559-10878578 | MsG0180000771.01.T01:CDS | 30.0% | |
| TTACAAATGGCTATATCCTT+CGG | - | Chr1:10878616-10878635 | MsG0180000771.01.T01:intron | 30.0% | |
| !!! | AAATCACATGTGGTTTTGTT+CGG | + | Chr1:10878831-10878850 | None:intergenic | 30.0% |
| CACTATATAGGAATCAGAGA+AGG | - | Chr1:10878963-10878982 | MsG0180000771.01.T01:intron | 35.0% | |
| CCAGACAAAAGCTACTTAAT+TGG | - | Chr1:10878582-10878601 | MsG0180000771.01.T01:intron | 35.0% | |
| GACAAAAGCTACTTAATTGG+TGG | - | Chr1:10878585-10878604 | MsG0180000771.01.T01:intron | 35.0% | |
| GGAGATTTATAACTTACCGA+AGG | + | Chr1:10878635-10878654 | None:intergenic | 35.0% | |
| GGTTCATAAATCAAAGGAAC+AGG | - | Chr1:10879034-10879053 | MsG0180000771.01.T01:intron | 35.0% | |
| ! | CCAATTAAGTAGCTTTTGTC+TGG | + | Chr1:10878585-10878604 | None:intergenic | 35.0% |
| !! | GCAAGTGGTTTTTCAATTCA+CGG | + | Chr1:10878480-10878499 | None:intergenic | 35.0% |
| ACAGAGTGCAGCTCTTTATA+TGG | - | Chr1:10879110-10879129 | MsG0180000771.01.T01:CDS | 40.0% | |
| ACCATAAACTGCATGTCTTC+GGG | + | Chr1:10879072-10879091 | None:intergenic | 40.0% | |
| AGACATGCAGTTTATGGTGA+TGG | - | Chr1:10879074-10879093 | MsG0180000771.01.T01:CDS | 40.0% | |
| AGTGCAGCTCTTTATATGGA+TGG | - | Chr1:10879114-10879133 | MsG0180000771.01.T01:CDS | 40.0% | |
| ATATAAAGAGCTGCACTCTG+TGG | + | Chr1:10879111-10879130 | None:intergenic | 40.0% | |
| CACCATAAACTGCATGTCTT+CGG | + | Chr1:10879073-10879092 | None:intergenic | 40.0% | |
| GAAGAGGATCAAGAGAATAG+TGG | - | Chr1:10878409-10878428 | MsG0180000771.01.T01:CDS | 40.0% | |
| GATGGTCATTACATAGCTGA+TGG | - | Chr1:10879132-10879151 | MsG0180000771.01.T01:CDS | 40.0% | |
| TAAATCAAAGGAACAGGCAC+TGG | - | Chr1:10879040-10879059 | MsG0180000771.01.T01:intron | 40.0% | |
| !! | ATGCAGTTTATGGTGATGGA+TGG | - | Chr1:10879078-10879097 | MsG0180000771.01.T01:CDS | 40.0% |
| ACCCGAAGACATGCAGTTTA+TGG | - | Chr1:10879068-10879087 | MsG0180000771.01.T01:CDS | 45.0% | |
| ATAAACTGCATGTCTTCGGG+TGG | + | Chr1:10879069-10879088 | None:intergenic | 45.0% | |
| ATTGGCTGAGTCTACAGGAT+TGG | - | Chr1:10878990-10879009 | MsG0180000771.01.T01:intron | 45.0% | |
| GAGGATCAAGAGAATAGTGG+TGG | - | Chr1:10878412-10878431 | MsG0180000771.01.T01:CDS | 45.0% | |
| GGAATCAGAGAAGGTAGCAT+TGG | - | Chr1:10878972-10878991 | MsG0180000771.01.T01:intron | 45.0% | |
| GGTGGGAGTTGCATTACAAA+TGG | - | Chr1:10878603-10878622 | MsG0180000771.01.T01:intron | 45.0% | |
| ! | AGCTTTTGTCTGGCCTCTTT+AGG | + | Chr1:10878575-10878594 | None:intergenic | 45.0% |
| ! | GAAGAGTGTGAGAAGAGAGT+AGG | + | Chr1:10878656-10878675 | None:intergenic | 45.0% |
| AATTCACGGTCTTCTGCTCG+AGG | + | Chr1:10878466-10878485 | None:intergenic | 50.0% | |
| CTGATGGTCCCTACCGTTTA+GGG | - | Chr1:10879148-10879167 | MsG0180000771.01.T01:CDS | 50.0% | |
| TCTGCTCGAGGATCAATCTC+TGG | + | Chr1:10878454-10878473 | None:intergenic | 50.0% | |
| ! | AGGCTCTGGTTCATCAGAAG+AGG | - | Chr1:10878393-10878412 | MsG0180000771.01.T01:CDS | 50.0% |
| !! | GTAGCATTGGCTGAGTCTAC+AGG | - | Chr1:10878985-10879004 | MsG0180000771.01.T01:intron | 50.0% |
| GCTGATGGTCCCTACCGTTT+AGG | - | Chr1:10879147-10879166 | MsG0180000771.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 10878382 | 10879176 | 10878382 | ID=MsG0180000771.01;Name=MsG0180000771.01 |
| Chr1 | mRNA | 10878382 | 10879176 | 10878382 | ID=MsG0180000771.01.T01;Parent=MsG0180000771.01;Name=MsG0180000771.01.T01;_AED=0.52;_eAED=0.53;_QI=0|0|0|1|0|0|2|0|112 |
| Chr1 | exon | 10879042 | 10879176 | 10879042 | ID=MsG0180000771.01.T01:exon:1093;Parent=MsG0180000771.01.T01 |
| Chr1 | exon | 10878382 | 10878585 | 10878382 | ID=MsG0180000771.01.T01:exon:1094;Parent=MsG0180000771.01.T01 |
| Chr1 | CDS | 10879042 | 10879176 | 10879042 | ID=MsG0180000771.01.T01:cds;Parent=MsG0180000771.01.T01 |
| Chr1 | CDS | 10878382 | 10878585 | 10878382 | ID=MsG0180000771.01.T01:cds;Parent=MsG0180000771.01.T01 |
| Gene Sequence |
| Protein sequence |
