AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001330.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001330.01.T01 MTR_1g035650 88.670 203 23 0 1 203 1 203 3.85e-126 359
MsG0180001330.01.T01 MTR_1g035620 55.369 298 103 9 1 285 1 281 1.18e-97 294
MsG0180001330.01.T01 MTR_1g050845 53.979 289 109 8 1 285 1 269 5.31e-84 270
MsG0180001330.01.T01 MTR_1g035640 48.684 304 139 6 1 298 1 293 9.20e-84 260
MsG0180001330.01.T01 MTR_1g035570 44.062 320 136 7 1 297 1 300 3.43e-76 239
MsG0180001330.01.T01 MTR_6g061070 39.748 317 159 8 9 294 18 333 1.15e-62 206
MsG0180001330.01.T01 MTR_5g044670 29.126 309 188 8 3 289 10 309 2.70e-37 135
MsG0180001330.01.T01 MTR_7g050580 33.677 291 170 10 6 283 10 290 1.74e-33 125
MsG0180001330.01.T01 MTR_7g050560 33.117 308 149 11 6 283 10 290 2.58e-33 125
MsG0180001330.01.T01 MTR_3g098790 29.831 295 193 7 6 287 10 303 2.56e-32 122
MsG0180001330.01.T01 MTR_7g050710 29.766 299 188 8 6 283 10 307 5.65e-32 121
MsG0180001330.01.T01 MTR_1g035490 42.138 159 76 2 133 289 58 202 4.23e-31 119
MsG0180001330.01.T01 MTR_1g033740 37.500 176 82 5 2 155 8 177 3.73e-28 108
MsG0180001330.01.T01 MTR_4g119990 28.814 295 172 10 9 273 15 301 8.84e-28 111
MsG0180001330.01.T01 MTR_7g050720 29.904 311 183 10 6 283 7 315 9.10e-28 110
MsG0180001330.01.T01 MTR_7g050590 31.429 280 170 8 25 283 2 280 1.25e-27 109
MsG0180001330.01.T01 MTR_8g103940 30.000 310 181 11 8 290 38 338 1.00e-26 110
MsG0180001330.01.T01 MTR_6g043890 28.966 290 172 9 9 273 15 295 6.35e-26 105
MsG0180001330.01.T01 MTR_1g035500 35.185 162 93 3 5 155 21 181 4.16e-25 100
MsG0180001330.01.T01 MTR_1g035480 36.842 171 88 5 2 155 3 170 7.01e-24 96.7
MsG0180001330.01.T01 MTR_3g061340 28.671 286 173 10 6 281 9 273 3.09e-22 94.4
MsG0180001330.01.T01 MTR_1g035520 26.230 305 167 10 25 281 88 382 3.98e-22 95.9
MsG0180001330.01.T01 MTR_1g108780 26.712 292 177 9 24 287 12 294 7.29e-21 92.8
MsG0180001330.01.T01 MTR_3g061320 24.820 278 199 5 6 281 9 278 1.61e-20 89.7
MsG0180001330.01.T01 MTR_3g061310 24.731 279 204 4 6 281 9 284 1.68e-20 89.7
MsG0180001330.01.T01 MTR_3g061350 26.027 292 183 8 6 281 9 283 7.85e-20 87.8
MsG0180001330.01.T01 MTR_1g035470 36.691 139 76 3 25 155 2 136 7.29e-19 82.0
MsG0180001330.01.T01 MTR_3g061290 38.583 127 69 3 6 130 9 128 1.86e-18 82.4
MsG0180001330.01.T01 MTR_3g061330 39.167 120 68 3 6 122 9 126 2.42e-18 83.2
MsG0180001330.01.T01 MTR_3g061370 26.578 301 166 9 8 281 8 280 2.65e-18 83.6
MsG0180001330.01.T01 MTR_1g035510 32.143 140 85 3 25 155 2 140 3.18e-17 79.3
MsG0180001330.01.T01 MTR_3g005420 28.214 280 170 7 7 284 287 537 6.19e-15 75.5
MsG0180001330.01.T01 MTR_5g070440 24.911 281 178 7 7 284 19 269 5.12e-14 71.2
MsG0180001330.01.T01 MTR_3g005490 25.000 284 169 9 7 286 19 262 7.27e-14 70.9
MsG0180001330.01.T01 MTR_7g050640 39.362 94 55 1 9 100 13 106 2.98e-13 65.9
MsG0180001330.01.T01 MTR_3g005480 24.468 282 168 9 7 283 19 260 4.03e-13 68.6
MsG0180001330.01.T01 MTR_1g035460 40.244 82 47 1 25 104 2 83 5.14e-13 69.3
MsG0180001330.01.T01 MTR_1g035440 38.776 98 57 2 9 103 15 112 1.22e-12 67.0
MsG0180001330.01.T01 MTR_3g005460 23.776 286 173 10 9 281 262 515 1.40e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001330.01.T01 AT4G01580 29.371 143 95 3 7 147 28 166 1.30e-14 71.6

Find 43 sgRNAs with CRISPR-Local

Find 71 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTAGCTTCACAAAATATT+TGG 0.130474 1:+19555639 None:intergenic
GTGCTAAACAAGATGTATTT+TGG 0.183994 1:-19555573 MsG0180001330.01.T01:CDS
TTATGCCAAAGGATGTTATT+TGG 0.223238 1:-19555035 MsG0180001330.01.T01:intron
TGTTTCAAAGTTGAAATATT+TGG 0.227189 1:-19555448 MsG0180001330.01.T01:CDS
ATTATCTTGAAGAACTTTCT+AGG 0.251633 1:+19555805 None:intergenic
TGACATTTCAACTTCAAAAC+TGG 0.265312 1:+19555063 None:intergenic
GAAAAGGTGGCAAGAGAAAA+AGG 0.281037 1:-19555312 MsG0180001330.01.T01:CDS
ACATCTTGTTTAGCACCATT+TGG 0.328942 1:+19555580 None:intergenic
CAAAATCAAGAACTGCAAAA+TGG 0.354682 1:-19555781 MsG0180001330.01.T01:CDS
AGCAAGAAATTATCATCCTT+TGG 0.369605 1:-19555092 MsG0180001330.01.T01:CDS
TGTAGCTTCACAAAATATTT+GGG 0.369839 1:+19555640 None:intergenic
ATTGCAAAATCTTTGATACT+TGG 0.372938 1:-19555505 MsG0180001330.01.T01:CDS
TGGAGCGTAATGGTGATATT+TGG 0.378752 1:-19555549 MsG0180001330.01.T01:CDS
AGAATAAAGACTACTGGATT+TGG 0.390473 1:+19555604 None:intergenic
CATCTTGTTTAGCACCATTT+GGG 0.395094 1:+19555581 None:intergenic
ACATACCAAATAACATCCTT+TGG 0.421503 1:+19555030 None:intergenic
TTGCAAAATCTTTGATACTT+GGG 0.433780 1:-19555504 MsG0180001330.01.T01:CDS
AAAATATATAATTTGAAACT+TGG 0.446327 1:-19554513 MsG0180001330.01.T01:CDS
GTAGTCTTTATTCTCCCAAA+TGG 0.456688 1:-19555595 MsG0180001330.01.T01:CDS
AACTTTGCAGGTTGTAATAG+AGG 0.470896 1:-19555169 MsG0180001330.01.T01:intron
TTATCTTGAAGAACTTTCTA+GGG 0.480715 1:+19555806 None:intergenic
TTTGGGAGAATAAAGACTAC+TGG 0.483895 1:+19555598 None:intergenic
GACAATGTTACTAATACTAA+TGG 0.484869 1:-19555355 MsG0180001330.01.T01:CDS
AGTACATCTCAAAGGAGAAA+AGG 0.489671 1:-19555328 MsG0180001330.01.T01:CDS
CTCCTCTCTTGCTCGAGTAA+TGG 0.510615 1:+19554437 None:intergenic
TTTAAGAAGTTGAAGTTGCA+AGG 0.514255 1:-19554408 MsG0180001330.01.T01:CDS
GACATTTCAACTTCAAAACT+GGG 0.526404 1:+19555064 None:intergenic
ATGGTAATAGTGAATAAAAG+TGG 0.534500 1:+19554456 None:intergenic
CATCTACTGTGACCACTGGT+TGG 0.552617 1:-19554548 MsG0180001330.01.T01:CDS
TAGAGGAGGCAAGATTAAGA+AGG 0.558763 1:-19555152 MsG0180001330.01.T01:CDS
AAATCATCTACTGTGACCAC+TGG 0.561649 1:-19554552 MsG0180001330.01.T01:CDS
AACTTCAACTTCTTAAAGCT+AGG 0.570828 1:+19554414 None:intergenic
TACCATTACTCGAGCAAGAG+AGG 0.576066 1:-19554439 MsG0180001330.01.T01:CDS
TAATAGTGAATAAAAGTGGT+TGG 0.584021 1:+19554460 None:intergenic
ATGTCACGATCTTATGCCAA+AGG 0.590157 1:-19555046 MsG0180001330.01.T01:CDS
TTCATCAACTCTAAATCTTG+CGG 0.597151 1:+19554617 None:intergenic
AATATTTGGTCGCAATACAG+TGG 0.606076 1:-19555434 MsG0180001330.01.T01:CDS
GCTAAATGACTTCCAACCAG+TGG 0.615117 1:+19554536 None:intergenic
ACATCTCAAAGGAGAAAAGG+TGG 0.624210 1:-19555325 MsG0180001330.01.T01:CDS
ATTTAGAGTTGATGAAGACA+TGG 0.625733 1:-19554610 MsG0180001330.01.T01:CDS
TTTGCAGGTTGTAATAGAGG+AGG 0.626297 1:-19555166 MsG0180001330.01.T01:intron
ATGGTGCTAGTACATCTCAA+AGG 0.644614 1:-19555336 MsG0180001330.01.T01:CDS
TATTGAAACAGAGGATACCA+AGG 0.667785 1:-19554677 MsG0180001330.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAATCCTTAAAAAAA+AGG + Chr1:19555387-19555406 None:intergenic 10.0%
!!! AAAATATATAATTTGAAACT+TGG - Chr1:19555654-19555673 MsG0180001330.01.T01:intron 10.0%
!!! TTTTATAAAAGTGTAAAAAA+TGG - Chr1:19555409-19555428 MsG0180001330.01.T01:CDS 10.0%
!!! ATATTTGGTTTCAAAAAAAT+TGG - Chr1:19554633-19554652 MsG0180001330.01.T01:CDS 15.0%
!!! GTTCTAGTTTTTAAATATAA+AGG - Chr1:19554689-19554708 MsG0180001330.01.T01:intron 15.0%
!!! TATTTGGTTTCAAAAAAATT+GGG - Chr1:19554634-19554653 MsG0180001330.01.T01:CDS 15.0%
!!! TATTTTAAGCCTTTTTTTTA+AGG - Chr1:19555375-19555394 MsG0180001330.01.T01:CDS 15.0%
!!! TGATTTTTTAACATTGTTAA+AGG + Chr1:19554457-19554476 None:intergenic 15.0%
!! AAAAATATTTATCTTGCAGA+GGG - Chr1:19554502-19554521 MsG0180001330.01.T01:CDS 20.0%
!! AAATACTTGAAAAAATTGGA+AGG - Chr1:19555525-19555544 MsG0180001330.01.T01:CDS 20.0%
!! AATACTTGAAAAAATTGGAA+GGG - Chr1:19555526-19555545 MsG0180001330.01.T01:CDS 20.0%
!! GAGAAAATACTTGAAAAAAT+TGG - Chr1:19555521-19555540 MsG0180001330.01.T01:CDS 20.0%
!! TGTTTCAAAGTTGAAATATT+TGG - Chr1:19554719-19554738 MsG0180001330.01.T01:intron 20.0%
!! TTCTCTTAAATATTGTTGAT+AGG + Chr1:19555335-19555354 None:intergenic 20.0%
!!! ACTTTCAAAATTTTCAAATC+TGG - Chr1:19555162-19555181 MsG0180001330.01.T01:CDS 20.0%
!!! CTAGTTTTTAAATATAAAGG+AGG - Chr1:19554692-19554711 MsG0180001330.01.T01:intron 20.0%
! ATGGTAATAGTGAATAAAAG+TGG + Chr1:19555714-19555733 None:intergenic 25.0%
! ATGTAGCTTCACAAAATATT+TGG + Chr1:19554531-19554550 None:intergenic 25.0%
! ATTATCTTGAAGAACTTTCT+AGG + Chr1:19554365-19554384 None:intergenic 25.0%
! ATTGCAAAATCTTTGATACT+TGG - Chr1:19554662-19554681 MsG0180001330.01.T01:CDS 25.0%
! CAAAAATATTTATCTTGCAG+AGG - Chr1:19554501-19554520 MsG0180001330.01.T01:CDS 25.0%
! GACAATGTTACTAATACTAA+TGG - Chr1:19554812-19554831 MsG0180001330.01.T01:intron 25.0%
! GCTAATCAGAAAAAAATCAA+AGG - Chr1:19554893-19554912 MsG0180001330.01.T01:intron 25.0%
! TAAGAGAAAACTCTCAAAAT+AGG - Chr1:19555347-19555366 MsG0180001330.01.T01:CDS 25.0%
! TAATAGTGAATAAAAGTGGT+TGG + Chr1:19555710-19555729 None:intergenic 25.0%
! TGTAGCTTCACAAAATATTT+GGG + Chr1:19554530-19554549 None:intergenic 25.0%
! TTATCTTGAAGAACTTTCTA+GGG + Chr1:19554364-19554383 None:intergenic 25.0%
! TTGCAAAATCTTTGATACTT+GGG - Chr1:19554663-19554682 MsG0180001330.01.T01:CDS 25.0%
!! AATATTTTGTGAAGCTACAT+TGG - Chr1:19554531-19554550 MsG0180001330.01.T01:CDS 25.0%
!! TATTTTCTCGAAAAATACCT+TGG + Chr1:19555510-19555529 None:intergenic 25.0%
!! TGCTAAACAAGATGTATTTT+GGG - Chr1:19554595-19554614 MsG0180001330.01.T01:CDS 25.0%
!!! TTCAGCTTCAACTTTTATTT+TGG + Chr1:19554771-19554790 None:intergenic 25.0%
!!! TTTTTTATGTGTAACTTTGC+AGG - Chr1:19554986-19555005 MsG0180001330.01.T01:intron 25.0%
AACTTCAACTTCTTAAAGCT+AGG + Chr1:19555756-19555775 None:intergenic 30.0%
ACAAACCAATATTGAAACAG+AGG - Chr1:19555481-19555500 MsG0180001330.01.T01:CDS 30.0%
ACATACCAAATAACATCCTT+TGG + Chr1:19555140-19555159 None:intergenic 30.0%
AGAATAAAGACTACTGGATT+TGG + Chr1:19554566-19554585 None:intergenic 30.0%
AGCAAGAAATTATCATCCTT+TGG - Chr1:19555075-19555094 MsG0180001330.01.T01:CDS 30.0%
CAAAATCAAGAACTGCAAAA+TGG - Chr1:19554386-19554405 MsG0180001330.01.T01:CDS 30.0%
GACATTTCAACTTCAAAACT+GGG + Chr1:19555106-19555125 None:intergenic 30.0%
TGACATTTCAACTTCAAAAC+TGG + Chr1:19555107-19555126 None:intergenic 30.0%
TTATGCCAAAGGATGTTATT+TGG - Chr1:19555132-19555151 MsG0180001330.01.T01:CDS 30.0%
TTCATCAACTCTAAATCTTG+CGG + Chr1:19555553-19555572 None:intergenic 30.0%
TTTAAGAAGTTGAAGTTGCA+AGG - Chr1:19555759-19555778 MsG0180001330.01.T01:intron 30.0%
! GTGCTAAACAAGATGTATTT+TGG - Chr1:19554594-19554613 MsG0180001330.01.T01:CDS 30.0%
!! ATTTAGAGTTGATGAAGACA+TGG - Chr1:19555557-19555576 MsG0180001330.01.T01:CDS 30.0%
!! TAAACAAGATGTATTTTGGG+TGG - Chr1:19554598-19554617 MsG0180001330.01.T01:CDS 30.0%
AACTTTGCAGGTTGTAATAG+AGG - Chr1:19554998-19555017 MsG0180001330.01.T01:intron 35.0%
AATATTTGGTCGCAATACAG+TGG - Chr1:19554733-19554752 MsG0180001330.01.T01:intron 35.0%
AGTACATCTCAAAGGAGAAA+AGG - Chr1:19554839-19554858 MsG0180001330.01.T01:intron 35.0%
GGTATCCTCTGTTTCAATAT+TGG + Chr1:19555489-19555508 None:intergenic 35.0%
GTAGTCTTTATTCTCCCAAA+TGG - Chr1:19554572-19554591 MsG0180001330.01.T01:CDS 35.0%
TATTGAAACAGAGGATACCA+AGG - Chr1:19555490-19555509 MsG0180001330.01.T01:CDS 35.0%
TGGCAAGAGAAAAAGGAATA+TGG - Chr1:19554862-19554881 MsG0180001330.01.T01:intron 35.0%
TTTGGGAGAATAAAGACTAC+TGG + Chr1:19554572-19554591 None:intergenic 35.0%
! ACATCTTGTTTAGCACCATT+TGG + Chr1:19554590-19554609 None:intergenic 35.0%
! CATCTTGTTTAGCACCATTT+GGG + Chr1:19554589-19554608 None:intergenic 35.0%
! TCAAAACTGGGATTTTCCAA+AGG + Chr1:19555094-19555113 None:intergenic 35.0%
AAATCATCTACTGTGACCAC+TGG - Chr1:19555615-19555634 MsG0180001330.01.T01:CDS 40.0%
ACATCTCAAAGGAGAAAAGG+TGG - Chr1:19554842-19554861 MsG0180001330.01.T01:intron 40.0%
ATGTCACGATCTTATGCCAA+AGG - Chr1:19555121-19555140 MsG0180001330.01.T01:CDS 40.0%
GAAAAGGTGGCAAGAGAAAA+AGG - Chr1:19554855-19554874 MsG0180001330.01.T01:intron 40.0%
TAGAGGAGGCAAGATTAAGA+AGG - Chr1:19555015-19555034 MsG0180001330.01.T01:intron 40.0%
TGGAGCGTAATGGTGATATT+TGG - Chr1:19554618-19554637 MsG0180001330.01.T01:CDS 40.0%
TTTGCAGGTTGTAATAGAGG+AGG - Chr1:19555001-19555020 MsG0180001330.01.T01:intron 40.0%
! ATGGTGCTAGTACATCTCAA+AGG - Chr1:19554831-19554850 MsG0180001330.01.T01:intron 40.0%
GCTAAATGACTTCCAACCAG+TGG + Chr1:19555634-19555653 None:intergenic 45.0%
TACCATTACTCGAGCAAGAG+AGG - Chr1:19555728-19555747 MsG0180001330.01.T01:intron 45.0%
!! GTATTTTGGGTGGAGCGTAA+TGG - Chr1:19554608-19554627 MsG0180001330.01.T01:CDS 45.0%
CATCTACTGTGACCACTGGT+TGG - Chr1:19555619-19555638 MsG0180001330.01.T01:CDS 50.0%
CTCCTCTCTTGCTCGAGTAA+TGG + Chr1:19555733-19555752 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 19554347 19555842 19554347 ID=MsG0180001330.01;Name=MsG0180001330.01
Chr1 mRNA 19554347 19555842 19554347 ID=MsG0180001330.01.T01;Parent=MsG0180001330.01;Name=MsG0180001330.01.T01;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|1|1|4|0|316
Chr1 exon 19555774 19555842 19555774 ID=MsG0180001330.01.T01:exon:25933;Parent=MsG0180001330.01.T01
Chr1 exon 19555275 19555668 19555275 ID=MsG0180001330.01.T01:exon:25932;Parent=MsG0180001330.01.T01
Chr1 exon 19555036 19555181 19555036 ID=MsG0180001330.01.T01:exon:25931;Parent=MsG0180001330.01.T01
Chr1 exon 19554347 19554688 19554347 ID=MsG0180001330.01.T01:exon:25930;Parent=MsG0180001330.01.T01
Chr1 CDS 19555774 19555842 19555774 ID=MsG0180001330.01.T01:cds;Parent=MsG0180001330.01.T01
Chr1 CDS 19555275 19555668 19555275 ID=MsG0180001330.01.T01:cds;Parent=MsG0180001330.01.T01
Chr1 CDS 19555036 19555181 19555036 ID=MsG0180001330.01.T01:cds;Parent=MsG0180001330.01.T01
Chr1 CDS 19554347 19554688 19554347 ID=MsG0180001330.01.T01:cds;Parent=MsG0180001330.01.T01
Gene Sequence

>MsG0180001330.01.T01

ATGTCATTTTATCACCCTAGAAAGTTCTTCAAGATAATGCAAAATCAAGAACTGCAAAATGGAGAGCTTAGGGTTCCCAAATATTTTGTGAAGCTACATTGGAAAAATGTACCAAATCCAGTAGTCTTTATTCTCCCAAATGGTGCTAAACAAGATGTATTTTGGGTGGAGCGTAATGGTGATATTTGGTTTCAAAAAAATTGGGAAAAAATTGCAAAATCTTTGATACTTGGGTATGTTCTAGTTTTTAAATATAAAGGAGGATCATGTTTCAAAGTTGAAATATTTGGTCGCAATACAGTGGAAATAGACTGTTCCAAAATAAAAGTTGAAGCTGAAGAAGTTGTTACTGTGAGTGATGACAATGTTACTAATACTAATGGTGCTAGTACATCTCAAAGGAGAAAAGGTGGCAAGAGAAAAAGGAATATGGATCTTGATGCTAATCAGAAAAAAATCAAAGGTTGTAATAGAGGAGGCAAGATTAAGAAGGCTAAAAAATGCTTAAGAACTGAAGCTGTTAAAGAGAGAGCAAGAAATTATCATCCTTTGGAAAATCCCAGTTTTGAAGTTGAAATGTCACGATCTTATGCCAAAGGATGTTATTTGAGGATACCAAGGTATTTTTCGAGAAAATACTTGAAAAAATTGGAAGGGACCGCAAGATTTAGAGTTGATGAAGACATGGCTATAGAAGTGAAGTTGAATTTTGATGATGCTACTAAATCATCTACTGTGACCACTGGTTGGAAGTCATTTAGCAAAATATATAATTTGAAACTTGGTGATGTGTGCAAATTTGTGATGACTCAATGCCAACCACTTTTATTCACTATTACCATTACTCGAGCAAGAGAGGAGCCTAGCTTTAAGAAGTTGAAGTTGCAAGGTTTTTCTTTCTTTCTTAATATATTTCTTTTAATATCTATAGTTTTTTTCTACACTTCTTAA

Protein sequence

>MsG0180001330.01.T01

MSFYHPRKFFKIMQNQELQNGELRVPKYFVKLHWKNVPNPVVFILPNGAKQDVFWVERNGDIWFQKNWEKIAKSLILGYVLVFKYKGGSCFKVEIFGRNTVEIDCSKIKVEAEEVVTVSDDNVTNTNGASTSQRRKGGKRKRNMDLDANQKKIKGCNRGGKIKKAKKCLRTEAVKERARNYHPLENPSFEVEMSRSYAKGCYLRIPRYFSRKYLKKLEGTARFRVDEDMAIEVKLNFDDATKSSTVTTGWKSFSKIYNLKLGDVCKFVMTQCQPLLFTITITRAREEPSFKKLKLQGFSFFLNIFLLISIVFFYTS*