AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001336.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001336.01.T01 MTR_6g061070 68.776 474 102 4 1 430 2 473 0.0 639
MsG0180001336.01.T01 MTR_1g035480 69.110 191 33 2 1 185 1 171 2.42e-86 261
MsG0180001336.01.T01 MTR_1g033740 68.254 189 38 2 2 185 7 178 8.11e-86 260
MsG0180001336.01.T01 MTR_1g035500 62.632 190 48 2 2 185 10 182 1.20e-79 244
MsG0180001336.01.T01 MTR_1g035520 48.065 310 122 8 32 315 87 383 1.20e-77 246
MsG0180001336.01.T01 MTR_1g035520 65.649 131 43 1 299 427 253 383 5.21e-51 177
MsG0180001336.01.T01 MTR_1g035570 37.376 404 226 10 26 426 14 393 9.14e-71 229
MsG0180001336.01.T01 MTR_1g035620 35.388 438 217 12 17 426 9 408 3.63e-65 214
MsG0180001336.01.T01 MTR_1g035640 40.323 310 145 8 26 327 14 291 7.63e-58 197
MsG0180001336.01.T01 MTR_1g035470 62.264 159 33 3 32 185 1 137 4.45e-57 184
MsG0180001336.01.T01 MTR_1g035510 59.236 157 43 2 32 184 1 140 6.27e-56 184
MsG0180001336.01.T01 MTR_1g050845 34.694 392 187 10 17 384 9 355 4.27e-47 173
MsG0180001336.01.T01 MTR_5g044670 29.345 351 202 10 2 337 5 324 3.13e-39 144
MsG0180001336.01.T01 MTR_1g035490 36.552 290 135 12 166 427 60 328 5.32e-39 143
MsG0180001336.01.T01 MTR_1g035650 39.631 217 103 5 17 230 9 200 6.10e-39 139
MsG0180001336.01.T01 MTR_1g035540 84.932 73 11 0 281 353 31 103 6.57e-37 132
MsG0180001336.01.T01 MTR_3g098790 31.269 323 179 15 11 318 7 301 2.05e-33 128
MsG0180001336.01.T01 MTR_7g050560 30.351 313 186 7 11 318 7 292 1.47e-31 123
MsG0180001336.01.T01 MTR_7g050720 32.188 320 179 13 16 315 9 310 3.50e-31 122
MsG0180001336.01.T01 MTR_7g050580 29.618 314 187 9 11 318 7 292 2.46e-30 119
MsG0180001336.01.T01 MTR_1g108780 27.974 311 196 10 33 335 13 303 2.49e-29 119
MsG0180001336.01.T01 MTR_7g050710 28.483 323 198 9 10 318 6 309 5.12e-29 116
MsG0180001336.01.T01 MTR_7g050590 29.693 293 189 6 32 318 1 282 1.20e-28 114
MsG0180001336.01.T01 MTR_8g103940 24.481 482 271 16 15 427 37 494 1.75e-28 117
MsG0180001336.01.T01 MTR_4g119990 29.091 330 204 13 12 327 10 323 1.93e-28 115
MsG0180001336.01.T01 MTR_6g043890 30.897 301 183 12 14 306 12 295 3.27e-27 111
MsG0180001336.01.T01 MTR_3g061350 29.276 304 177 11 17 314 12 283 5.09e-25 104
MsG0180001336.01.T01 MTR_1g035460 26.959 319 195 11 32 330 1 301 8.16e-24 102
MsG0180001336.01.T01 MTR_3g061370 27.244 312 187 11 10 314 2 280 1.03e-23 100
MsG0180001336.01.T01 MTR_3g061310 26.299 308 186 11 15 314 10 284 1.18e-23 100
MsG0180001336.01.T01 MTR_3g061320 27.157 313 187 13 7 314 2 278 3.43e-23 99.0
MsG0180001336.01.T01 MTR_3g061340 27.103 321 174 15 7 315 2 274 8.70e-21 92.0
MsG0180001336.01.T01 MTR_3g061290 34.507 142 88 3 15 153 10 149 9.47e-19 84.7
MsG0180001336.01.T01 MTR_1g035440 43.689 103 54 2 14 112 12 114 1.07e-17 82.8
MsG0180001336.01.T01 MTR_3g005460 23.171 328 182 11 1 315 246 516 1.43e-17 85.1
MsG0180001336.01.T01 MTR_3g061330 30.952 168 102 4 7 162 2 167 7.69e-17 80.1
MsG0180001336.01.T01 MTR_3g005420 26.045 311 162 12 16 318 288 538 3.75e-16 80.9
MsG0180001336.01.T01 MTR_5g070440 24.841 314 170 11 11 317 15 269 6.09e-16 78.2
MsG0180001336.01.T01 MTR_1g021330 23.780 328 205 11 15 302 24 346 5.43e-15 76.6
MsG0180001336.01.T01 MTR_3g061220 36.207 116 61 4 15 129 10 113 5.67e-15 71.2
MsG0180001336.01.T01 MTR_7g050640 37.000 100 60 2 11 107 7 106 1.57e-14 70.1
MsG0180001336.01.T01 MTR_1g021360 23.891 293 176 9 28 302 193 456 1.58e-14 75.9
MsG0180001336.01.T01 MTR_1g021440 23.193 332 204 11 15 302 21 345 1.82e-14 75.1
MsG0180001336.01.T01 MTR_3g005495 28.079 203 112 6 16 199 242 429 6.69e-14 73.9
MsG0180001336.01.T01 MTR_3g005480 33.962 106 67 2 10 112 14 119 1.71e-13 70.9
MsG0180001336.01.T01 MTR_3g005490 32.710 107 69 2 9 112 13 119 1.02e-12 68.6
MsG0180001336.01.T01 MTR_1g021400 22.785 316 211 10 15 302 28 338 1.14e-12 69.3
MsG0180001336.01.T01 MTR_3g005440 34.000 100 63 2 16 112 241 340 7.20e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001336.01.T01 AT3G18990 24.332 337 206 11 17 313 6 333 1.05e-18 87.4
MsG0180001336.01.T01 AT3G18990 24.332 337 206 11 17 313 6 333 2.02e-18 87.0
MsG0180001336.01.T01 AT4G33280 23.385 325 196 15 18 315 20 318 5.91e-13 70.1

Find 81 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATTCAACTTTGTGAATTGC+AGG 0.285867 1:-19604783 MsG0180001336.01.T01:CDS
GGGCATGGTTCCTTTGTGGC+TGG 0.290615 1:+19602278 None:intergenic
TATATTGGATGATAGTAGTT+TGG 0.295324 1:-19603536 MsG0180001336.01.T01:CDS
TTGAATTTGTCATGTAAATT+CGG 0.311912 1:+19602036 None:intergenic
TATATTTAGTGGAAATTTAG+TGG 0.337369 1:-19601866 MsG0180001336.01.T01:CDS
AAGAGACATAATATATTTAG+TGG 0.341164 1:-19601877 MsG0180001336.01.T01:CDS
GGTCTGAGTTCAAAATGAAT+TGG 0.350012 1:-19603672 MsG0180001336.01.T01:CDS
CAAACATTCTTCTAAACATC+TGG 0.359210 1:+19601664 None:intergenic
ATTCACAAAGTTGAATGGTT+TGG 0.365673 1:+19604789 None:intergenic
TACAACGCGGTGACGATGTT+TGG 0.370214 1:-19603648 MsG0180001336.01.T01:CDS
TTATAACCTTCAATGTGATA+TGG 0.376198 1:+19602913 None:intergenic
CTCTGGGCATGGTTCCTTTG+TGG 0.384680 1:+19602274 None:intergenic
AATCTGACCCTCCTTTGTAT+TGG 0.385935 1:+19603569 None:intergenic
CTGCAATTCACAAAGTTGAA+TGG 0.392583 1:+19604784 None:intergenic
GGCATGGTTCCTTTGTGGCT+GGG 0.402651 1:+19602279 None:intergenic
ACGATGTTTGGCTTCAGAAC+TGG 0.404512 1:-19603636 MsG0180001336.01.T01:CDS
CATTGAAGGTTATAATAGAT+TGG 0.414338 1:-19602905 MsG0180001336.01.T01:intron
CGTGCAAAATCATGTATTCA+AGG 0.415158 1:-19601698 MsG0180001336.01.T01:CDS
GAAATACCTTGAAACTGCTC+TGG 0.423422 1:+19602257 None:intergenic
TATTCTTTCGAAAACCATCT+AGG 0.429204 1:+19602515 None:intergenic
ATAGTAATTGTGAATGAAAG+TGG 0.438268 1:+19602305 None:intergenic
TATGATCAACATCTCGATCA+TGG 0.447909 1:-19601802 MsG0180001336.01.T01:CDS
TTAGAAGTGTTGGTGAGGAC+AGG 0.455257 1:-19602460 MsG0180001336.01.T01:CDS
GTCTGAGTTCAAAATGAATT+GGG 0.455385 1:-19603671 MsG0180001336.01.T01:CDS
GAATTTACATGACAAATTCA+AGG 0.456040 1:-19602034 MsG0180001336.01.T01:CDS
TTATCGCCATATCACATTGA+AGG 0.463256 1:-19602919 MsG0180001336.01.T01:CDS
CTAATAGAAACTGTGGAGGC+CGG 0.465574 1:-19603038 MsG0180001336.01.T01:intron
AGAGATCGCAAAGTGAAGAT+TGG 0.467048 1:-19601748 MsG0180001336.01.T01:CDS
AAGGAACATGACTTGAAAGT+TGG 0.470859 1:-19602362 MsG0180001336.01.T01:CDS
TGTCTCTCTACGAATTTCCT+TGG 0.471061 1:+19603739 None:intergenic
TAATAGAAACTGTGGAGGCC+GGG 0.483673 1:-19603037 MsG0180001336.01.T01:intron
TCAATGTCAAGTGTTCACTA+TGG 0.491973 1:-19604845 MsG0180001336.01.T01:CDS
TGTCTCTTCAACAGCTCACT+TGG 0.493789 1:+19601892 None:intergenic
TGAAACAGATAATACCTAGA+TGG 0.494737 1:-19602529 MsG0180001336.01.T01:intron
GTGGAGTTGAAAGCAGAAGA+AGG 0.502821 1:-19601847 MsG0180001336.01.T01:CDS
TCACAAATTATCATCCCAAT+GGG 0.508213 1:-19602963 MsG0180001336.01.T01:CDS
TTGCCAATGTTTGTCCCTTG+AGG 0.510253 1:+19603352 None:intergenic
TTCACAAATTATCATCCCAA+TGG 0.514529 1:-19602964 MsG0180001336.01.T01:CDS
ACATCGCTTAGACTTCCAAA+TGG 0.515725 1:-19603694 MsG0180001336.01.T01:CDS
CCATGCCCAGAGCAGTTTCA+AGG 0.516067 1:-19602263 MsG0180001336.01.T01:intron
TGGACGTGGGAGTGAAGCTA+AGG 0.519368 1:-19604825 MsG0180001336.01.T01:CDS
GGAAATTCGTAGAGAGACAT+TGG 0.530097 1:-19603735 MsG0180001336.01.T01:CDS
TTTGGAAGTCTAAGCGATGT+TGG 0.531226 1:+19603697 None:intergenic
TTTGGCTTCAGAACTGGAAG+AGG 0.532764 1:-19603630 MsG0180001336.01.T01:CDS
CCTTGAAACTGCTCTGGGCA+TGG 0.538312 1:+19602263 None:intergenic
CAATGATGAACAAGAAGCTA+AGG 0.539148 1:-19603488 MsG0180001336.01.T01:CDS
GATCATGGGTGGTTCACTAA+AGG 0.540814 1:-19601787 MsG0180001336.01.T01:CDS
AGAAGAAGGAACATGGTTTG+TGG 0.547977 1:-19601833 MsG0180001336.01.T01:CDS
ATTTCTTAGCTTTCTTACCC+CGG 0.551754 1:+19603019 None:intergenic
AATAGAAACTGTGGAGGCCG+GGG 0.552110 1:-19603036 MsG0180001336.01.T01:intron
ACTAGTGCTGCTACACCTCA+AGG 0.555707 1:-19603367 MsG0180001336.01.T01:CDS
CATCGCTTAGACTTCCAAAT+GGG 0.555762 1:-19603693 MsG0180001336.01.T01:CDS
ATGATCAACATCTCGATCAT+GGG 0.558223 1:-19601801 MsG0180001336.01.T01:CDS
TGTTCTTGCAGATGATTCCA+AGG 0.559130 1:-19603756 MsG0180001336.01.T01:intron
TAGAAGTGTTGGTGAGGACA+GGG 0.562489 1:-19602459 MsG0180001336.01.T01:CDS
GATAATTTGACAGTGAAACT+AGG 0.566923 1:+19602937 None:intergenic
TCAAGTGTTCACTATGGACG+TGG 0.567496 1:-19604839 MsG0180001336.01.T01:CDS
CTCACCAACACTTCTAATTG+TGG 0.571224 1:+19602466 None:intergenic
AAGGCCACAATTAGAAGTGT+TGG 0.571434 1:-19602470 MsG0180001336.01.T01:CDS
GTGAAACTAGGACTCCCATT+GGG 0.573485 1:+19602949 None:intergenic
ACACCTCAAGGGACAAACAT+TGG 0.575666 1:-19603355 MsG0180001336.01.T01:CDS
TTTGCGCGCTCTCTCAGATG+TGG 0.586381 1:-19603607 MsG0180001336.01.T01:CDS
AGTGAAACTAGGACTCCCAT+TGG 0.586614 1:+19602948 None:intergenic
ATGGGTGGTTCACTAAAGGT+TGG 0.588689 1:-19601783 MsG0180001336.01.T01:CDS
AAATACCTTGAAACTGCTCT+GGG 0.589681 1:+19602258 None:intergenic
CACAATTAGAAGTGTTGGTG+AGG 0.602624 1:-19602465 MsG0180001336.01.T01:CDS
GAAAATGATGATGAATGTGA+AGG 0.603394 1:+19604879 None:intergenic
TGAAAGCAGAAGAAGGAACA+TGG 0.607559 1:-19601840 MsG0180001336.01.T01:CDS
AAAATGATGATGAATGTGAA+GGG 0.618789 1:+19604880 None:intergenic
ATCAACATCTCGATCATGGG+TGG 0.629808 1:-19601798 MsG0180001336.01.T01:CDS
GGACGTGGGAGTGAAGCTAA+GGG 0.640577 1:-19604824 MsG0180001336.01.T01:CDS
GCAGAGGCTAATAGAAACTG+TGG 0.644824 1:-19603045 MsG0180001336.01.T01:intron
TACTATTATCCCAGCCACAA+AGG 0.646969 1:-19602288 MsG0180001336.01.T01:CDS
GAATGAGTTATTGCAACAGA+AGG 0.647927 1:-19602489 MsG0180001336.01.T01:CDS
GTGTTGGTGAGGACAGGGCA+TGG 0.648324 1:-19602454 MsG0180001336.01.T01:CDS
CTAGTGCTGCTACACCTCAA+GGG 0.659036 1:-19603366 MsG0180001336.01.T01:CDS
AGAAGGAACATGGTTTGTGG+AGG 0.660933 1:-19601830 MsG0180001336.01.T01:CDS
TGAATGCAAAGTGAAGATTG+AGG 0.693015 1:-19601528 MsG0180001336.01.T01:three_prime_UTR
GAGGCTAATAGAAACTGTGG+AGG 0.696340 1:-19603042 MsG0180001336.01.T01:intron
CAAGTGTTCACTATGGACGT+GGG 0.700697 1:-19604838 MsG0180001336.01.T01:CDS
AAAATGAATTGGGTACAACG+CGG 0.771281 1:-19603661 MsG0180001336.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTTATTCAATAATTAAAT+TGG + Chr1:19601754-19601773 None:intergenic 10.0%
!! GTTTATTCAATAATTAAATT+GGG + Chr1:19601753-19601772 None:intergenic 10.0%
!!! TTTAGGTATATTTTAATTAT+TGG - Chr1:19603753-19603772 MsG0180001336.01.T01:intron 10.0%
!!! AGTTTTTGAAATTAAATTCA+TGG - Chr1:19602337-19602356 MsG0180001336.01.T01:CDS 15.0%
!!! TATTGAATAAACTTTGTTAT+TGG - Chr1:19601761-19601780 MsG0180001336.01.T01:CDS 15.0%
!! AAGAGACATAATATATTTAG+TGG - Chr1:19604498-19604517 MsG0180001336.01.T01:intron 20.0%
!! AATTTGTATGCATTAAAAAG+TGG + Chr1:19603134-19603153 None:intergenic 20.0%
!! ACATTGACAAAAATGTTATT+AGG - Chr1:19603100-19603119 MsG0180001336.01.T01:intron 20.0%
!! TAAAAAATACGTAAATACAG+CGG + Chr1:19603193-19603212 None:intergenic 20.0%
!! TACGGATTTATTATCATTAA+TGG - Chr1:19601962-19601981 MsG0180001336.01.T01:intron 20.0%
!! TATATTTAGTGGAAATTTAG+TGG - Chr1:19604509-19604528 MsG0180001336.01.T01:intron 20.0%
!! TATTTACAAGAGAAACATTT+GGG + Chr1:19603602-19603621 None:intergenic 20.0%
!! TTGAATTTGTCATGTAAATT+CGG + Chr1:19604342-19604361 None:intergenic 20.0%
!!! AGATTTTCATGTCATTATAT+TGG - Chr1:19602824-19602843 MsG0180001336.01.T01:intron 20.0%
!!! ATCATGTGTAACAAATTTTA+AGG - Chr1:19604200-19604219 MsG0180001336.01.T01:intron 20.0%
!!! CAAAATTTTAGGTAGAAATT+GGG + Chr1:19601920-19601939 None:intergenic 20.0%
!!! GTTTTTAGTGTCAAAATTTT+AGG + Chr1:19601931-19601950 None:intergenic 20.0%
!!! TCAAAATTTTAGGTAGAAAT+TGG + Chr1:19601921-19601940 None:intergenic 20.0%
!!! TTATAATTGCTTTGATTTGA+TGG - Chr1:19601834-19601853 MsG0180001336.01.T01:CDS 20.0%
!!! TTTTGAAATTAAATTCATGG+AGG - Chr1:19602340-19602359 MsG0180001336.01.T01:CDS 20.0%
! AAAAAATACGTAAATACAGC+GGG + Chr1:19603192-19603211 None:intergenic 25.0%
! AAGTAACAATAGAGACATTT+AGG - Chr1:19603736-19603755 MsG0180001336.01.T01:CDS 25.0%
! AATGATAATAAATCCGTACA+TGG + Chr1:19601960-19601979 None:intergenic 25.0%
! AATGCACAAAAACTAAAGAT+AGG - Chr1:19603376-19603395 MsG0180001336.01.T01:CDS 25.0%
! AATTGGGTCAATGATTAAAA+TGG + Chr1:19601737-19601756 None:intergenic 25.0%
! ACTAACAGAAAACATATTCA+AGG - Chr1:19603640-19603659 MsG0180001336.01.T01:CDS 25.0%
! ATAGTAATTGTGAATGAAAG+TGG + Chr1:19604073-19604092 None:intergenic 25.0%
! ATTGGGTCAATGATTAAAAT+GGG + Chr1:19601736-19601755 None:intergenic 25.0%
! ATTTACAAGAGAAACATTTG+GGG + Chr1:19603601-19603620 None:intergenic 25.0%
! CATTAATGGAATTCATCATT+TGG - Chr1:19601976-19601995 MsG0180001336.01.T01:intron 25.0%
! CATTGAAGGTTATAATAGAT+TGG - Chr1:19603470-19603489 MsG0180001336.01.T01:CDS 25.0%
! CATTTCTAATTGAAACTCAT+TGG - Chr1:19602586-19602605 MsG0180001336.01.T01:intron 25.0%
! CTATTTACAAGAGAAACATT+TGG + Chr1:19603603-19603622 None:intergenic 25.0%
! GAATTTACATGACAAATTCA+AGG - Chr1:19604341-19604360 MsG0180001336.01.T01:intron 25.0%
! TACAAGAAATCTGAACTAAT+TGG + Chr1:19602416-19602435 None:intergenic 25.0%
! TACTTCAATAATCGAAAGAA+TGG + Chr1:19601669-19601688 None:intergenic 25.0%
! TATATTGGATGATAGTAGTT+TGG - Chr1:19602839-19602858 MsG0180001336.01.T01:intron 25.0%
! TGTTTGATACATATATGCTT+TGG - Chr1:19602472-19602491 MsG0180001336.01.T01:CDS 25.0%
! TTATAACCTTCAATGTGATA+TGG + Chr1:19603465-19603484 None:intergenic 25.0%
!! AAAATGATGATGAATGTGAA+GGG + Chr1:19601498-19601517 None:intergenic 25.0%
!! TTCACATTCATCATCATTTT+CGG - Chr1:19601498-19601517 MsG0180001336.01.T01:three_prime_UTR 25.0%
!! TTCTCTTGTAAATAGTCTTA+TGG - Chr1:19603608-19603627 MsG0180001336.01.T01:CDS 25.0%
!! TTGATTTTCTTTTTCTTGAG+GGG + Chr1:19602972-19602991 None:intergenic 25.0%
!!! AGTTGATTTTCTTTTTCTTG+AGG + Chr1:19602974-19602993 None:intergenic 25.0%
!!! CTTTTAGTTTTCCAATACAA+AGG - Chr1:19602795-19602814 MsG0180001336.01.T01:intron 25.0%
!!! GTTGATTTTCTTTTTCTTGA+GGG + Chr1:19602973-19602992 None:intergenic 25.0%
!!! TAGTTTTTAAAGTTTTGCAG+AGG - Chr1:19603314-19603333 MsG0180001336.01.T01:CDS 25.0%
AATCATACCAACTCTTTAAG+TGG - Chr1:19602226-19602245 MsG0180001336.01.T01:intron 30.0%
AATCGAAAGAATGGATGAAA+TGG + Chr1:19601660-19601679 None:intergenic 30.0%
ATAAGTATATGTAACGTGCA+TGG + Chr1:19603787-19603806 None:intergenic 30.0%
ATCTAGGTATTATCTGTTTC+AGG + Chr1:19603847-19603866 None:intergenic 30.0%
ATTCACAAAGTTGAATGGTT+TGG + Chr1:19601589-19601608 None:intergenic 30.0%
CAAACATTCTTCTAAACATC+TGG + Chr1:19604714-19604733 None:intergenic 30.0%
GAATACGAAGATTTGAATGT+TGG - Chr1:19601791-19601810 MsG0180001336.01.T01:CDS 30.0%
GATAATTTGACAGTGAAACT+AGG + Chr1:19603441-19603460 None:intergenic 30.0%
GTAGTATGTTTACATTGACA+AGG + Chr1:19604236-19604255 None:intergenic 30.0%
GTCTGAGTTCAAAATGAATT+GGG - Chr1:19602704-19602723 MsG0180001336.01.T01:intron 30.0%
TAAAAACAAACAGCCATGTA+CGG - Chr1:19601944-19601963 MsG0180001336.01.T01:intron 30.0%
TAATGGAATTCATCATTTGG+TGG - Chr1:19601979-19601998 MsG0180001336.01.T01:intron 30.0%
TATTCTTTCGAAAACCATCT+AGG + Chr1:19603863-19603882 None:intergenic 30.0%
TCACAAATTATCATCCCAAT+GGG - Chr1:19603412-19603431 MsG0180001336.01.T01:CDS 30.0%
TGAAACAGATAATACCTAGA+TGG - Chr1:19603846-19603865 MsG0180001336.01.T01:intron 30.0%
TTCACAAATTATCATCCCAA+TGG - Chr1:19603411-19603430 MsG0180001336.01.T01:CDS 30.0%
TTTGTTCTTATATATGCAGC+TGG - Chr1:19604452-19604471 MsG0180001336.01.T01:intron 30.0%
! CATGATCAAAACAGTATTGA+GGG + Chr1:19602289-19602308 None:intergenic 30.0%
! GAAAATGATGATGAATGTGA+AGG + Chr1:19601499-19601518 None:intergenic 30.0%
! GTTCACGTCTTGAATTTTAA+TGG + Chr1:19603248-19603267 None:intergenic 30.0%
! TAGTTTTCCAATACAAAGGA+GGG - Chr1:19602799-19602818 MsG0180001336.01.T01:intron 30.0%
! TGGGTATTTTCATGATCATT+CGG + Chr1:19602945-19602964 None:intergenic 30.0%
! TTTTGTAACATCATTCGACA+AGG - Chr1:19604365-19604384 MsG0180001336.01.T01:intron 30.0%
!! AGAGGCTTTCATTTTTCTAT+AGG - Chr1:19603546-19603565 MsG0180001336.01.T01:CDS 30.0%
!! TAATTGCTTTGATTTGATGG+AGG - Chr1:19601837-19601856 MsG0180001336.01.T01:CDS 30.0%
!! TTAGTTTTCCAATACAAAGG+AGG - Chr1:19602798-19602817 MsG0180001336.01.T01:intron 30.0%
!!! ATCTGGTTTTCTTAGGTATT+TGG - Chr1:19601878-19601897 MsG0180001336.01.T01:CDS 30.0%
AAAATGAATTGGGTACAACG+CGG - Chr1:19602714-19602733 MsG0180001336.01.T01:intron 35.0%
AAACAGTCCACTTAAAGAGT+TGG + Chr1:19602236-19602255 None:intergenic 35.0%
AAATACCTTGAAACTGCTCT+GGG + Chr1:19604120-19604139 None:intergenic 35.0%
AAATGATAAAAAGGGCAACC+CGG + Chr1:19602177-19602196 None:intergenic 35.0%
AAGGAACATGACTTGAAAGT+TGG - Chr1:19604013-19604032 MsG0180001336.01.T01:intron 35.0%
ACTGATTAAGCAAACTGTGT+TGG + Chr1:19602500-19602519 None:intergenic 35.0%
AGCTCCACCAAATGATAAAA+AGG + Chr1:19602186-19602205 None:intergenic 35.0%
ATGATCAACATCTCGATCAT+GGG - Chr1:19604574-19604593 MsG0180001336.01.T01:intron 35.0%
ATGTTGCATACTTGCTTATC+TGG - Chr1:19601861-19601880 MsG0180001336.01.T01:CDS 35.0%
ATTCTACACATTCACACACA+TGG + Chr1:19604427-19604446 None:intergenic 35.0%
CAATGATGAACAAGAAGCTA+AGG - Chr1:19602887-19602906 MsG0180001336.01.T01:intron 35.0%
CACACACATGGCTATATAAA+TGG + Chr1:19604415-19604434 None:intergenic 35.0%
CAGTTAGGTACAAATATTGC+TGG + Chr1:19603522-19603541 None:intergenic 35.0%
CATTCAACTTTGTGAATTGC+AGG - Chr1:19601592-19601611 MsG0180001336.01.T01:three_prime_UTR 35.0%
CCATGATCAAAACAGTATTG+AGG + Chr1:19602290-19602309 None:intergenic 35.0%
CGTGCAAAATCATGTATTCA+AGG - Chr1:19604677-19604696 MsG0180001336.01.T01:intron 35.0%
CTGATTAAGCAAACTGTGTT+GGG + Chr1:19602499-19602518 None:intergenic 35.0%
CTGCAATTCACAAAGTTGAA+TGG + Chr1:19601594-19601613 None:intergenic 35.0%
GAATGAGTTATTGCAACAGA+AGG - Chr1:19603886-19603905 MsG0180001336.01.T01:intron 35.0%
GCTCCACCAAATGATAAAAA+GGG + Chr1:19602185-19602204 None:intergenic 35.0%
GGTCTGAGTTCAAAATGAAT+TGG - Chr1:19602703-19602722 MsG0180001336.01.T01:intron 35.0%
TATGATCAACATCTCGATCA+TGG - Chr1:19604573-19604592 MsG0180001336.01.T01:intron 35.0%
TCAATGTCAAGTGTTCACTA+TGG - Chr1:19601530-19601549 MsG0180001336.01.T01:three_prime_UTR 35.0%
TCTTGTGTAAAAACAGGGTA+AGG - Chr1:19602085-19602104 MsG0180001336.01.T01:intron 35.0%
TGAATGCAAAGTGAAGATTG+AGG - Chr1:19604847-19604866 MsG0180001336.01.T01:exon 35.0%
TTATCGCCATATCACATTGA+AGG - Chr1:19603456-19603475 MsG0180001336.01.T01:CDS 35.0%
TTTGTACCTAACTGTGTAAG+AGG - Chr1:19603528-19603547 MsG0180001336.01.T01:CDS 35.0%
! ACACAGTTTGCTTAATCAGT+CGG - Chr1:19602500-19602519 MsG0180001336.01.T01:CDS 35.0%
! ATTTCTTAGCTTTCTTACCC+CGG + Chr1:19603359-19603378 None:intergenic 35.0%
! ATTTTGAACTCAGACCCATT+TGG + Chr1:19602699-19602718 None:intergenic 35.0%
! GGTTTTCTTAGGTATTTGGA+TGG - Chr1:19601882-19601901 MsG0180001336.01.T01:CDS 35.0%
! GTATTATCTGTTTCAGGTCA+TGG + Chr1:19603841-19603860 None:intergenic 35.0%
! TTGCCCTTTTTATCATTTGG+TGG - Chr1:19602179-19602198 MsG0180001336.01.T01:intron 35.0%
!! AGCAGTTTTATAATGCAGGT+TGG - Chr1:19603974-19603993 MsG0180001336.01.T01:intron 35.0%
!! CAAGAGCAGTTTTATAATGC+AGG - Chr1:19603970-19603989 MsG0180001336.01.T01:intron 35.0%
!! CTCTTTAAGTGGACTGTTTA+AGG - Chr1:19602237-19602256 MsG0180001336.01.T01:intron 35.0%
!! GACTGCATGTTTTTAATCCT+AGG - Chr1:19603166-19603185 MsG0180001336.01.T01:intron 35.0%
!! TGGTTGGAAGTCATTTTCAA+AGG - Chr1:19603994-19604013 MsG0180001336.01.T01:intron 35.0%
!!! CCTCAATACTGTTTTGATCA+TGG - Chr1:19602287-19602306 MsG0180001336.01.T01:CDS 35.0%
!!! CTTGCTTATCTGGTTTTCTT+AGG - Chr1:19601871-19601890 MsG0180001336.01.T01:CDS 35.0%
!!! GTTTTATAATGCAGGTTGGT+TGG - Chr1:19603978-19603997 MsG0180001336.01.T01:intron 35.0%
!!! TTACCCTGTTTTTACACAAG+AGG + Chr1:19602086-19602105 None:intergenic 35.0%
AATCTGACCCTCCTTTGTAT+TGG + Chr1:19602809-19602828 None:intergenic 40.0%
ACAGCCTCTTGTGTAAAAAC+AGG - Chr1:19602079-19602098 MsG0180001336.01.T01:intron 40.0%
ACATCGCTTAGACTTCCAAA+TGG - Chr1:19602681-19602700 MsG0180001336.01.T01:intron 40.0%
AGAAGAAGGAACATGGTTTG+TGG - Chr1:19604542-19604561 MsG0180001336.01.T01:intron 40.0%
AGAGATCGCAAAGTGAAGAT+TGG - Chr1:19604627-19604646 MsG0180001336.01.T01:intron 40.0%
CAGCCTCTTGTGTAAAAACA+GGG - Chr1:19602080-19602099 MsG0180001336.01.T01:intron 40.0%
CATCGCTTAGACTTCCAAAT+GGG - Chr1:19602682-19602701 MsG0180001336.01.T01:intron 40.0%
CGTACAATACACCAAATGGT+GGG - Chr1:19602111-19602130 MsG0180001336.01.T01:intron 40.0%
CTCACCAACACTTCTAATTG+TGG + Chr1:19603912-19603931 None:intergenic 40.0%
GAAATACCTTGAAACTGCTC+TGG + Chr1:19604121-19604140 None:intergenic 40.0%
GCATCCTTGCAAAAAGTTTC+AGG - Chr1:19603065-19603084 MsG0180001336.01.T01:intron 40.0%
GGAAATTCGTAGAGAGACAT+TGG - Chr1:19602640-19602659 MsG0180001336.01.T01:intron 40.0%
GTTGTTGTCATGTGACTGTA+AGG - Chr1:19602035-19602054 MsG0180001336.01.T01:CDS 40.0%
TACTATTATCCCAGCCACAA+AGG - Chr1:19604087-19604106 MsG0180001336.01.T01:intron 40.0%
TCTTGTATTAGTGCTACGCA+TGG - Chr1:19602429-19602448 MsG0180001336.01.T01:CDS 40.0%
TGAAAGCAGAAGAAGGAACA+TGG - Chr1:19604535-19604554 MsG0180001336.01.T01:intron 40.0%
TGAAAGCCTCTTACACAGTT+AGG + Chr1:19603537-19603556 None:intergenic 40.0%
TGTCTCTCTACGAATTTCCT+TGG + Chr1:19602639-19602658 None:intergenic 40.0%
TGTTCTTGCAGATGATTCCA+AGG - Chr1:19602619-19602638 MsG0180001336.01.T01:intron 40.0%
TTTGGAAGTCTAAGCGATGT+TGG + Chr1:19602681-19602700 None:intergenic 40.0%
! AAGGCCACAATTAGAAGTGT+TGG - Chr1:19603905-19603924 MsG0180001336.01.T01:intron 40.0%
! CTTGCCTGAAACTTTTTGCA+AGG + Chr1:19603072-19603091 None:intergenic 40.0%
! CTTTTTCTTGAGGGGTTGTT+GGG + Chr1:19602964-19602983 None:intergenic 40.0%
! GGGTTGCCCTTTTTATCATT+TGG - Chr1:19602176-19602195 MsG0180001336.01.T01:intron 40.0%
! GGTATGATTGAGAGTACAAG+TGG + Chr1:19602215-19602234 None:intergenic 40.0%
! TCTTTTTCTTGAGGGGTTGT+TGG + Chr1:19602965-19602984 None:intergenic 40.0%
!! CACAATTAGAAGTGTTGGTG+AGG - Chr1:19603910-19603929 MsG0180001336.01.T01:intron 40.0%
ACACCTCAAGGGACAAACAT+TGG - Chr1:19603020-19603039 MsG0180001336.01.T01:CDS 45.0%
AGTGAAACTAGGACTCCCAT+TGG + Chr1:19603430-19603449 None:intergenic 45.0%
ATCAACATCTCGATCATGGG+TGG - Chr1:19604577-19604596 MsG0180001336.01.T01:intron 45.0%
ATGGGTGGTTCACTAAAGGT+TGG - Chr1:19604592-19604611 MsG0180001336.01.T01:intron 45.0%
ATTAGTGCTACGCATGGCTT+TGG - Chr1:19602435-19602454 MsG0180001336.01.T01:CDS 45.0%
CAAGTGTTCACTATGGACGT+GGG - Chr1:19601537-19601556 MsG0180001336.01.T01:three_prime_UTR 45.0%
CTAATAGAAACTGTGGAGGC+CGG - Chr1:19603337-19603356 MsG0180001336.01.T01:CDS 45.0%
GAGGCTAATAGAAACTGTGG+AGG - Chr1:19603333-19603352 MsG0180001336.01.T01:CDS 45.0%
GATCATGGGTGGTTCACTAA+AGG - Chr1:19604588-19604607 MsG0180001336.01.T01:intron 45.0%
GCAGAGGCTAATAGAAACTG+TGG - Chr1:19603330-19603349 MsG0180001336.01.T01:CDS 45.0%
GCGTACAATACACCAAATGG+TGG - Chr1:19602110-19602129 MsG0180001336.01.T01:intron 45.0%
GCTGCGTACAATACACCAAA+TGG - Chr1:19602107-19602126 MsG0180001336.01.T01:intron 45.0%
GTCATGTGACTGTAAGGTCA+CGG - Chr1:19602041-19602060 MsG0180001336.01.T01:CDS 45.0%
GTGAAACTAGGACTCCCATT+GGG + Chr1:19603429-19603448 None:intergenic 45.0%
GTGGAGTTGAAAGCAGAAGA+AGG - Chr1:19604528-19604547 MsG0180001336.01.T01:intron 45.0%
TAATAGAAACTGTGGAGGCC+GGG - Chr1:19603338-19603357 MsG0180001336.01.T01:CDS 45.0%
TCAAGTGTTCACTATGGACG+TGG - Chr1:19601536-19601555 MsG0180001336.01.T01:three_prime_UTR 45.0%
TCATGTGACTGTAAGGTCAC+GGG - Chr1:19602042-19602061 MsG0180001336.01.T01:CDS 45.0%
TGTCTCTTCAACAGCTCACT+TGG + Chr1:19604486-19604505 None:intergenic 45.0%
TTGCCAATGTTTGTCCCTTG+AGG + Chr1:19603026-19603045 None:intergenic 45.0%
! ACGATGTTTGGCTTCAGAAC+TGG - Chr1:19602739-19602758 MsG0180001336.01.T01:intron 45.0%
! AGAAGGAACATGGTTTGTGG+AGG - Chr1:19604545-19604564 MsG0180001336.01.T01:intron 45.0%
! TTACACAAGAGGCTGTTTCC+AGG + Chr1:19602075-19602094 None:intergenic 45.0%
! TTTGGCTTCAGAACTGGAAG+AGG - Chr1:19602745-19602764 MsG0180001336.01.T01:intron 45.0%
!! TAGAAGTGTTGGTGAGGACA+GGG - Chr1:19603916-19603935 MsG0180001336.01.T01:intron 45.0%
!! TTAGAAGTGTTGGTGAGGAC+AGG - Chr1:19603915-19603934 MsG0180001336.01.T01:intron 45.0%
AATAGAAACTGTGGAGGCCG+GGG - Chr1:19603339-19603358 MsG0180001336.01.T01:CDS 50.0%
CAACTTTACCAGTTGCGCCA+AGG + Chr1:19602019-19602038 None:intergenic 50.0%
TACAACGCGGTGACGATGTT+TGG - Chr1:19602727-19602746 MsG0180001336.01.T01:intron 50.0%
TACGTAAATACAGCGGGCCT+AGG + Chr1:19603186-19603205 None:intergenic 50.0%
! ACTAGTGCTGCTACACCTCA+AGG - Chr1:19603008-19603027 MsG0180001336.01.T01:CDS 50.0%
! ATCATTTGGTGGAGCTCGAG+GGG - Chr1:19601990-19602009 MsG0180001336.01.T01:intron 50.0%
! CATCATTTGGTGGAGCTCGA+GGG - Chr1:19601989-19602008 MsG0180001336.01.T01:intron 50.0%
! CTAGTGCTGCTACACCTCAA+GGG - Chr1:19603009-19603028 MsG0180001336.01.T01:CDS 50.0%
! TCATCATTTGGTGGAGCTCG+AGG - Chr1:19601988-19602007 MsG0180001336.01.T01:intron 50.0%
AAGGTCACGGGTTCAAGTCC+TGG - Chr1:19602054-19602073 MsG0180001336.01.T01:intron 55.0%
CCATGCCCAGAGCAGTTTCA+AGG - Chr1:19604112-19604131 MsG0180001336.01.T01:intron 55.0%
CTCTGGGCATGGTTCCTTTG+TGG + Chr1:19604104-19604123 None:intergenic 55.0%
GGACGTGGGAGTGAAGCTAA+GGG - Chr1:19601551-19601570 MsG0180001336.01.T01:three_prime_UTR 55.0%
GGCATGGTTCCTTTGTGGCT+GGG + Chr1:19604099-19604118 None:intergenic 55.0%
TGGACGTGGGAGTGAAGCTA+AGG - Chr1:19601550-19601569 MsG0180001336.01.T01:three_prime_UTR 55.0%
TTTGCGCGCTCTCTCAGATG+TGG - Chr1:19602768-19602787 MsG0180001336.01.T01:intron 55.0%
! CCTTGAAACTGCTCTGGGCA+TGG + Chr1:19604115-19604134 None:intergenic 55.0%
ACTGAAGCTCCCGCATACGC+AGG + Chr1:19602151-19602170 None:intergenic 60.0%
AGGGGTAACCTTGGCGCAAC+TGG - Chr1:19602008-19602027 MsG0180001336.01.T01:intron 60.0%
ATGCGGGAGCTTCAGTGCAC+CGG - Chr1:19602155-19602174 MsG0180001336.01.T01:intron 60.0%
CTGAAGCTCCCGCATACGCA+GGG + Chr1:19602150-19602169 None:intergenic 60.0%
GGGCATGGTTCCTTTGTGGC+TGG + Chr1:19604100-19604119 None:intergenic 60.0%
TGGAGCTCGAGGGGTAACCT+TGG - Chr1:19601999-19602018 MsG0180001336.01.T01:intron 60.0%
! CAAATGGTGGGACCCCTTCC+CGG - Chr1:19602123-19602142 MsG0180001336.01.T01:intron 60.0%
! GTGTTGGTGAGGACAGGGCA+TGG - Chr1:19603921-19603940 MsG0180001336.01.T01:intron 60.0%
CATACGCAGGGTCCGGGAAG+GGG + Chr1:19602138-19602157 None:intergenic 65.0%
CGGGAAGGGGTCCCACCATT+TGG + Chr1:19602125-19602144 None:intergenic 65.0%
CTTCCCGGACCCTGCGTATG+CGG - Chr1:19602138-19602157 MsG0180001336.01.T01:intron 65.0%
GCATACGCAGGGTCCGGGAA+GGG + Chr1:19602139-19602158 None:intergenic 65.0%
TGCGGGAGCTTCAGTGCACC+GGG - Chr1:19602156-19602175 MsG0180001336.01.T01:intron 65.0%
TTCCCGGACCCTGCGTATGC+GGG - Chr1:19602139-19602158 MsG0180001336.01.T01:intron 65.0%
CGCATACGCAGGGTCCGGGA+AGG + Chr1:19602140-19602159 None:intergenic 70.0%
CTCCCGCATACGCAGGGTCC+GGG + Chr1:19602144-19602163 None:intergenic 70.0%
GCTCCCGCATACGCAGGGTC+CGG + Chr1:19602145-19602164 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 19601489 19604908 19601489 ID=MsG0180001336.01;Name=MsG0180001336.01
Chr1 mRNA 19601489 19604908 19601489 ID=MsG0180001336.01.T01;Parent=MsG0180001336.01;Name=MsG0180001336.01.T01;_AED=0.19;_eAED=0.19;_QI=44|0.6|0.5|1|1|1|6|186|430
Chr1 exon 19601489 19601926 19601489 ID=MsG0180001336.01.T01:exon:14449;Parent=MsG0180001336.01.T01
Chr1 exon 19602011 19602069 19602011 ID=MsG0180001336.01.T01:exon:14448;Parent=MsG0180001336.01.T01
Chr1 exon 19602264 19602543 19602264 ID=MsG0180001336.01.T01:exon:14447;Parent=MsG0180001336.01.T01
Chr1 exon 19602906 19603053 19602906 ID=MsG0180001336.01.T01:exon:14446;Parent=MsG0180001336.01.T01
Chr1 exon 19603307 19603767 19603307 ID=MsG0180001336.01.T01:exon:14445;Parent=MsG0180001336.01.T01
Chr1 exon 19604772 19604908 19604772 ID=MsG0180001336.01.T01:exon:14444;Parent=MsG0180001336.01.T01
Chr1 five_prime_UTR 19604865 19604908 19604865 ID=MsG0180001336.01.T01:five_prime_utr;Parent=MsG0180001336.01.T01
Chr1 CDS 19604772 19604864 19604772 ID=MsG0180001336.01.T01:cds;Parent=MsG0180001336.01.T01
Chr1 CDS 19603307 19603767 19603307 ID=MsG0180001336.01.T01:cds;Parent=MsG0180001336.01.T01
Chr1 CDS 19602906 19603053 19602906 ID=MsG0180001336.01.T01:cds;Parent=MsG0180001336.01.T01
Chr1 CDS 19602264 19602543 19602264 ID=MsG0180001336.01.T01:cds;Parent=MsG0180001336.01.T01
Chr1 CDS 19602011 19602069 19602011 ID=MsG0180001336.01.T01:cds;Parent=MsG0180001336.01.T01
Chr1 CDS 19601675 19601926 19601675 ID=MsG0180001336.01.T01:cds;Parent=MsG0180001336.01.T01
Chr1 three_prime_UTR 19601489 19601674 19601489 ID=MsG0180001336.01.T01:three_prime_utr;Parent=MsG0180001336.01.T01
Gene Sequence

>MsG0180001336.01.T01

ATGTCAAGTGTTCACTATGGACGTGGGAGTGAAGCTAAGGGAATGCATTTTTACCAAACCATTCAACTTTGTGAATTGCAGGATGAAGAGCTTATGATTCCAAGGAAATTCGTAGAGAGACATTGGAAAGATGTATCAAATCCAACATCGCTTAGACTTCCAAATGGGTCTGAGTTCAAAATGAATTGGGTACAACGCGGTGACGATGTTTGGCTTCAGAACTGGAAGAGGTTTGCGCGCTCTCTCAGATGTGGAGATCTTTTAGTTTTCCAATACAAAGGAGGGTCAGATTTTCATGTCATTATATTGGATGATAGTAGTTTGGAAATTGACTATTCATGTATGAGATGCAATGATGAACAAGAAGCTAAGGAGAGTGATGATGATGATGACTGTGTTGAGATTCCGAATGATCATGAAAATACCCAACAACCCCTCAAGAAAAAGAAAATCAACTCAAATGACATTGCTACTAGTGCTGCTACACCTCAAGGGACAAACATTGGCAAGAGAAAAATTAATATCAATGCATCCTTGCAAAAAGTTTCAGGCAAAAACTGTGGAGGCCGGGGTAAGAAAGCTAAGAAATGCACAAAAACTAAAGATAGGAATGAGAGAAGTTTCACAAATTATCATCCCAATGGGAGTCCTAGTTTCACTGTCAAATTATCGCCATATCACATTGAAGGTTATAATAGATTGATAATACCTAGATGGTTTTCGAAAGAATACATGAATGAGTTATTGCAACAGAAGGCCACAATTAGAAGTGTTGGTGAGGACAGGGCATGGTACGTGACGTTATACTTTGATAAAAGCAAGAGCAGTTTTATAATGCAGGTTGGTTGGAAGTCATTTTCAAAGGAACATGACTTGAAAGTTGGTGATGTATGTAAATTTGAGATGACTCAACGCAAACCACTTTCATTCACAATTACTATTATCCCAGCCACAAAGGAACCATGCCCAGAGCAGTTTCAAGATTCTGTAAGTCCGAATTTACATGACAAATTCAAGGTTTTTGTAACATCATTCGACAAGCTGGTTGTACTACCAAGTGAGCTGTTGAAGAGACATAATATATTTAGTGGAAATTTAGTGGAGTTGAAAGCAGAAGAAGGAACATGGTTTGTGGAGGTGAATTATGATCAACATCTCGATCATGGGTGGTTCACTAAAGGTTGGCAAGAATTTATGAGAGATCGCAAAGTGAAGATTGGAGACACTTGTTTGTTTGAGATGATTGACGTGCAAAATCATGTATTCAAGGTTTCAATTATTCCAGATGTTTAG
Protein sequence

>MsG0180001336.01.T01

MSSVHYGRGSEAKGMHFYQTIQLCELQDEELMIPRKFVERHWKDVSNPTSLRLPNGSEFKMNWVQRGDDVWLQNWKRFARSLRCGDLLVFQYKGGSDFHVIILDDSSLEIDYSCMRCNDEQEAKESDDDDDCVEIPNDHENTQQPLKKKKINSNDIATSAATPQGTNIGKRKININASLQKVSGKNCGGRGKKAKKCTKTKDRNERSFTNYHPNGSPSFTVKLSPYHIEGYNRLIIPRWFSKEYMNELLQQKATIRSVGEDRAWYVTLYFDKSKSSFIMQVGWKSFSKEHDLKVGDVCKFEMTQRKPLSFTITIIPATKEPCPEQFQDSVSPNLHDKFKVFVTSFDKLVVLPSELLKRHNIFSGNLVELKAEEGTWFVEVNYDQHLDHGWFTKGWQEFMRDRKVKIGDTCLFEMIDVQNHVFKVSIIPDV*