AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001348.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001348.01.T01 MTR_1g035620 88.571 245 27 1 1 245 1 244 7.03e-157 446
MsG0180001348.01.T01 MTR_1g035620 81.416 113 20 1 237 348 302 414 6.43e-60 198
MsG0180001348.01.T01 MTR_1g050845 84.146 246 24 2 1 245 1 232 1.47e-134 403
MsG0180001348.01.T01 MTR_1g050845 69.333 75 22 1 237 310 290 364 1.45e-26 111
MsG0180001348.01.T01 MTR_1g035570 41.220 410 164 10 1 346 1 397 1.10e-90 277
MsG0180001348.01.T01 MTR_1g035640 48.594 249 112 6 1 240 1 242 2.99e-70 226
MsG0180001348.01.T01 MTR_1g035650 55.024 209 75 4 1 196 1 203 7.97e-69 214
MsG0180001348.01.T01 MTR_6g061070 32.676 355 179 11 9 313 18 362 5.04e-37 139
MsG0180001348.01.T01 MTR_1g033740 41.104 163 75 4 9 156 22 178 1.99e-32 120
MsG0180001348.01.T01 MTR_1g035520 28.097 331 189 8 25 342 88 382 3.62e-30 119
MsG0180001348.01.T01 MTR_1g035500 36.810 163 91 4 5 156 21 182 3.10e-27 106
MsG0180001348.01.T01 MTR_1g035480 38.994 159 82 5 9 156 17 171 5.40e-27 105
MsG0180001348.01.T01 MTR_3g098790 32.287 223 134 6 6 212 10 231 2.56e-26 107
MsG0180001348.01.T01 MTR_7g050710 32.340 235 124 8 6 212 10 237 2.90e-26 107
MsG0180001348.01.T01 MTR_5g044670 30.189 265 157 8 3 245 10 268 1.29e-24 102
MsG0180001348.01.T01 MTR_1g035510 38.571 140 76 4 25 155 2 140 2.95e-24 99.4
MsG0180001348.01.T01 MTR_1g035510 51.786 56 27 0 243 298 167 222 5.04e-12 65.1
MsG0180001348.01.T01 MTR_7g050720 27.042 355 197 12 6 344 7 315 5.65e-24 100
MsG0180001348.01.T01 MTR_7g050580 30.769 221 123 6 6 208 10 218 2.23e-23 99.0
MsG0180001348.01.T01 MTR_6g043890 25.272 368 191 11 9 343 15 331 1.42e-22 97.1
MsG0180001348.01.T01 MTR_1g035470 48.315 89 45 1 25 113 2 89 8.87e-22 90.1
MsG0180001348.01.T01 MTR_7g050560 28.800 250 140 9 6 232 10 244 1.00e-21 94.4
MsG0180001348.01.T01 MTR_3g061290 33.523 176 105 5 6 177 9 176 7.92e-20 86.7
MsG0180001348.01.T01 MTR_8g103940 24.895 237 146 6 2 207 32 267 7.69e-19 87.8
MsG0180001348.01.T01 MTR_3g061330 27.184 206 139 4 6 202 9 212 9.66e-19 84.7
MsG0180001348.01.T01 MTR_3g061310 26.601 203 129 5 6 200 9 199 6.52e-18 83.2
MsG0180001348.01.T01 MTR_3g061320 34.109 129 75 3 6 132 9 129 2.77e-17 81.3
MsG0180001348.01.T01 MTR_4g119990 27.027 259 143 10 9 230 15 264 2.90e-16 79.3
MsG0180001348.01.T01 MTR_7g050590 27.619 210 122 4 25 208 2 207 2.99e-16 78.6
MsG0180001348.01.T01 MTR_3g061370 31.333 150 85 4 1 140 1 142 5.02e-16 77.8
MsG0180001348.01.T01 MTR_1g035490 43.137 102 54 2 248 347 233 332 6.07e-16 78.2
MsG0180001348.01.T01 MTR_1g035490 38.182 110 54 5 139 242 58 159 1.44e-13 71.2
MsG0180001348.01.T01 MTR_7g050640 41.758 91 51 1 8 96 12 102 9.07e-16 73.2
MsG0180001348.01.T01 MTR_3g061350 24.510 204 142 4 6 199 9 210 2.04e-15 75.9
MsG0180001348.01.T01 MTR_1g108780 23.796 353 163 11 24 344 12 290 2.65e-15 77.0
MsG0180001348.01.T01 MTR_1g035440 37.000 100 60 2 9 105 15 114 3.08e-14 72.0
MsG0180001348.01.T01 MTR_3g061340 32.500 120 74 5 6 120 9 126 3.72e-13 69.3
MsG0180001348.01.T01 MTR_1g034210 25.138 362 235 10 9 344 7 358 9.63e-13 68.9
MsG0180001348.01.T01 MTR_1g035460 23.348 227 126 6 25 207 2 224 2.52e-12 67.8
MsG0180001348.01.T01 MTR_1g021270 22.741 343 202 9 23 333 29 340 4.13e-12 67.8
MsG0180001348.01.T01 MTR_4g068320 36.364 99 61 1 9 105 6 104 2.99e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001348.01.T01 AT3G18990 22.254 355 241 7 9 348 6 340 6.45e-17 81.3
MsG0180001348.01.T01 AT3G18990 22.507 351 237 7 9 344 6 336 1.09e-16 80.9
MsG0180001348.01.T01 AT1G49475 33.051 118 70 5 9 123 34 145 6.36e-11 61.2

Find 60 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTTGCAGATGACCCTTTA+AGG 0.106528 1:+19758735 None:intergenic
GTGCTCAACAGGAAATATTT+TGG 0.162791 1:-19759665 MsG0180001348.01.T01:CDS
AATATCTTAACCTTAAAATA+TGG 0.178478 1:+19759543 None:intergenic
TGCAGGTTGGCATAAATTTA+AGG 0.202920 1:-19757652 MsG0180001348.01.T01:CDS
TTACGCTCATGGCTATATTT+TGG 0.211055 1:-19759120 MsG0180001348.01.T01:intron
ATATTTGGTTTAAGAGAAAT+TGG 0.232188 1:-19759626 MsG0180001348.01.T01:CDS
AGAAATTTGTGGAGAAATAT+TGG 0.241294 1:-19759728 MsG0180001348.01.T01:CDS
TTTGGGAGTCTAAGAATTAC+TGG 0.267570 1:+19759690 None:intergenic
TATTTGAATGACAACCTTAA+AGG 0.276541 1:-19758749 MsG0180001348.01.T01:CDS
AGTCTAAGAATTACTGGATT+TGG 0.299817 1:+19759696 None:intergenic
ATATTTCTCCACAAATTTCT+TGG 0.318034 1:+19759731 None:intergenic
ACTATCTTCAAGAACTCTTC+TGG 0.329140 1:+19759983 None:intergenic
TGGAGCGTGACGGTGATATT+TGG 0.333381 1:-19759641 MsG0180001348.01.T01:CDS
AACTTCACCTCCACTTTCAT+AGG 0.353581 1:+19758692 None:intergenic
ACAAAGGAGGCATAATTAAG+AGG 0.367124 1:-19759217 MsG0180001348.01.T01:CDS
ATTTCCTGTTGAGCACCATT+TGG 0.373513 1:+19759672 None:intergenic
ATTTGAATGACAACCTTAAA+GGG 0.391511 1:-19758748 MsG0180001348.01.T01:CDS
TCGAAATCATGAACTACATA+AGG 0.397577 1:-19759960 MsG0180001348.01.T01:CDS
ACTCCCAAATGGTGCTCAAC+AGG 0.399322 1:-19759676 MsG0180001348.01.T01:CDS
TATTTCTCCACAAATTTCTT+GGG 0.405795 1:+19759732 None:intergenic
TTTCCTGTTGAGCACCATTT+GGG 0.415520 1:+19759673 None:intergenic
TCATCTGCAAGCATTAGATT+TGG 0.416468 1:-19758725 MsG0180001348.01.T01:CDS
AAATTCGTGCAGTTCACAAA+TGG 0.417597 1:-19757729 MsG0180001348.01.T01:CDS
GAAGATTGCGGATTGAAGAT+TGG 0.417689 1:-19757630 MsG0180001348.01.T01:CDS
GACAATTCAAGTTCAAAACT+TGG 0.430631 1:+19759149 None:intergenic
AATTCGTGCAGTTCACAAAT+GGG 0.433283 1:-19757728 MsG0180001348.01.T01:CDS
ATTAATCAGCAAAGAATCTC+TGG 0.443135 1:-19759366 MsG0180001348.01.T01:intron
ATATCTTAACCTTAAAATAT+GGG 0.446492 1:+19759544 None:intergenic
ACACGAATCTCGATTGTGTT+CGG 0.462954 1:+19757897 None:intergenic
AATATTATGAGAGAACTTGT+AGG 0.465524 1:-19757680 MsG0180001348.01.T01:CDS
GAGAGTTCCCAAGAAATTTG+TGG 0.469633 1:-19759739 MsG0180001348.01.T01:CDS
TTTGCAAAGTCTCTAAAAGA+TGG 0.473533 1:-19759597 MsG0180001348.01.T01:CDS
TGTGTTTCAGCAGACTTAGA+AGG 0.481816 1:-19757936 MsG0180001348.01.T01:intron
TATTTGGTTTAAGAGAAATT+GGG 0.491470 1:-19759625 MsG0180001348.01.T01:CDS
GATATTTGGTCACAATACAT+TGG 0.502848 1:-19759526 MsG0180001348.01.T01:CDS
CGAAATCATGAACTACATAA+GGG 0.505471 1:-19759959 MsG0180001348.01.T01:CDS
AGAACTTGTAGGTTCTATGC+AGG 0.508431 1:-19757669 MsG0180001348.01.T01:CDS
AGAACATGCATTGTCACCTC+TGG 0.519227 1:-19758650 MsG0180001348.01.T01:intron
CATAAATTTAAGGAAGATTG+CGG 0.529511 1:-19757642 MsG0180001348.01.T01:CDS
CTTGTAGGTTCTATGCAGGT+TGG 0.536785 1:-19757665 MsG0180001348.01.T01:CDS
TTCATAGATGAAAGTTGTGA+AGG 0.538273 1:-19759480 MsG0180001348.01.T01:CDS
CAAAGGAGGCATAATTAAGA+GGG 0.540734 1:-19759216 MsG0180001348.01.T01:CDS
GTAATTCTTAGACTCCCAAA+TGG 0.561023 1:-19759687 MsG0180001348.01.T01:CDS
AACTTTGCAGGTAGGAACAA+AGG 0.563812 1:-19759233 MsG0180001348.01.T01:intron
GATGGCAATGTTAATAGTGC+TGG 0.575875 1:-19759423 MsG0180001348.01.T01:CDS
TTGTCACAATCTTACGCTCA+TGG 0.577647 1:-19759131 MsG0180001348.01.T01:CDS
TATGCTCTTGAAAAGTCACT+TGG 0.596669 1:+19758770 None:intergenic
AAGAGACATAAAGAGTGTCA+TGG 0.604494 1:-19757753 MsG0180001348.01.T01:CDS
TGAGAACATGCCTATGAAAG+TGG 0.608998 1:-19758702 MsG0180001348.01.T01:CDS
ACTTAGAAGGTGGTAATGCG+AGG 0.619480 1:-19757923 MsG0180001348.01.T01:CDS
TTCGTGCAGTTCACAAATGG+GGG 0.622353 1:-19757726 MsG0180001348.01.T01:CDS
ATTCGTGCAGTTCACAAATG+GGG 0.622898 1:-19757727 MsG0180001348.01.T01:CDS
GTTTCAGCAGACTTAGAAGG+TGG 0.625758 1:-19757933 MsG0180001348.01.T01:intron
ATAGTGCTGGCACATCTCAA+AGG 0.625943 1:-19759410 MsG0180001348.01.T01:CDS
CTTAGAAGGTGGTAATGCGA+GGG 0.643834 1:-19757922 MsG0180001348.01.T01:CDS
TCGTGTGAACTCTACTTACC+CGG 0.650490 1:-19757880 MsG0180001348.01.T01:intron
GCAGAACAAACAACTAGTGG+TGG 0.662011 1:+19757538 None:intergenic
GAAGTTGTTGAACTGAGTGA+TGG 0.670925 1:-19759441 MsG0180001348.01.T01:CDS
TTTGCAGGTAGGAACAAAGG+AGG 0.713031 1:-19759230 MsG0180001348.01.T01:intron
GAACATGCCTATGAAAGTGG+AGG 0.736568 1:-19758699 MsG0180001348.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACAAATTATTTAATATTTCA+AGG - Chr1:19759441-19759460 MsG0180001348.01.T01:CDS 10.0%
!! CAAAATAAAATAAAATTACA+AGG + Chr1:19758252-19758271 None:intergenic 10.0%
!!! AAATAATTTGTAATATATGA+GGG + Chr1:19759432-19759451 None:intergenic 10.0%
!!! TAAATAATTTGTAATATATG+AGG + Chr1:19759433-19759452 None:intergenic 10.0%
!!! TGTAATTTTATTTTATTTTG+AGG - Chr1:19758252-19758271 MsG0180001348.01.T01:intron 10.0%
!! AAAAAATATAACTTACAAGT+TGG - Chr1:19758938-19758957 MsG0180001348.01.T01:intron 15.0%
!! AATATCTTAACCTTAAAATA+TGG + Chr1:19758009-19758028 None:intergenic 15.0%
!! ACAATAATCATTTATGAATA+AGG + Chr1:19757782-19757801 None:intergenic 15.0%
!! ATATCTTAACCTTAAAATAT+GGG + Chr1:19758008-19758027 None:intergenic 15.0%
!! ATTATTAACAAATGTATAGT+TGG + Chr1:19757712-19757731 None:intergenic 15.0%
!!! AAAAAAGTTACTAGTAATTT+TGG - Chr1:19758711-19758730 MsG0180001348.01.T01:CDS 15.0%
!!! AACTTGTAAGTTATATTTTT+TGG + Chr1:19758939-19758958 None:intergenic 15.0%
!!! ATAAAATTTAAAACAGAACT+TGG + Chr1:19759372-19759391 None:intergenic 15.0%
!!! ATTTTTCAGAGATTTTAAAT+TGG - Chr1:19759248-19759267 MsG0180001348.01.T01:intron 15.0%
!!! CTTTTTTAAATAAAGTTTGA+AGG + Chr1:19759041-19759060 None:intergenic 15.0%
!!! TAAGTATAATGTTTTATAGT+AGG + Chr1:19758459-19758478 None:intergenic 15.0%
!!! TATTTTAAGGTTAAGATATT+TGG - Chr1:19758009-19758028 MsG0180001348.01.T01:intron 15.0%
!!! TTCATCTATGAATTTTATAT+TGG + Chr1:19758061-19758080 None:intergenic 15.0%
!! CAATTGATATTAAGCAAATT+TGG + Chr1:19759090-19759109 None:intergenic 20.0%
!! TCTATTGTGATGATAATATT+TGG - Chr1:19759156-19759175 MsG0180001348.01.T01:CDS 20.0%
!! TGAAATAGCCAATAAATAAT+AGG + Chr1:19758655-19758674 None:intergenic 20.0%
!!! AAGTTATATTTTTTGGCAAA+TGG + Chr1:19758932-19758951 None:intergenic 20.0%
!!! ATATTTGGTTTAAGAGAAAT+TGG - Chr1:19757923-19757942 MsG0180001348.01.T01:CDS 20.0%
!!! ATCTATTTTAGCCATTTATA+AGG + Chr1:19758285-19758304 None:intergenic 20.0%
!!! CTTGAACTAGTTTTTTTAAT+TGG + Chr1:19759469-19759488 None:intergenic 20.0%
!!! GCTTTAGTTTTCAAATATTT+AGG - Chr1:19757979-19757998 MsG0180001348.01.T01:intron 20.0%
!!! TAACTAAGTTTTCTTACTTT+AGG - Chr1:19759319-19759338 MsG0180001348.01.T01:intron 20.0%
!!! TATTTGGTTTAAGAGAAATT+GGG - Chr1:19757924-19757943 MsG0180001348.01.T01:CDS 20.0%
!!! TTAGTTTTCAAATATTTAGG+TGG - Chr1:19757982-19758001 MsG0180001348.01.T01:intron 20.0%
!!! TTTTTGTTTTTTAACTTTGC+AGG - Chr1:19758304-19758323 MsG0180001348.01.T01:intron 20.0%
! AAGTTTCAAAGGTTACATAT+AGG + Chr1:19758578-19758597 None:intergenic 25.0%
! AATATTATGAGAGAACTTGT+AGG - Chr1:19759869-19759888 MsG0180001348.01.T01:intron 25.0%
! AGAAATTTGTGGAGAAATAT+TGG - Chr1:19757821-19757840 MsG0180001348.01.T01:intron 25.0%
! AGGATTACTTATGTTAAATG+TGG - Chr1:19759693-19759712 MsG0180001348.01.T01:CDS 25.0%
! ATATTTCTCCACAAATTTCT+TGG + Chr1:19757821-19757840 None:intergenic 25.0%
! ATTTGAATGACAACCTTAAA+GGG - Chr1:19758801-19758820 MsG0180001348.01.T01:intron 25.0%
! CATAAATTTAAGGAAGATTG+CGG - Chr1:19759907-19759926 MsG0180001348.01.T01:intron 25.0%
! GATAATATTTGGAAGAAAAG+AGG - Chr1:19759167-19759186 MsG0180001348.01.T01:CDS 25.0%
! GTATTTGGCCTATTATTTAT+TGG - Chr1:19758644-19758663 MsG0180001348.01.T01:intron 25.0%
! TAAACCTTGAATTGAAATAG+AGG - Chr1:19758753-19758772 MsG0180001348.01.T01:CDS 25.0%
! TATTTCTCCACAAATTTCTT+GGG + Chr1:19757820-19757839 None:intergenic 25.0%
! TATTTGAATGACAACCTTAA+AGG - Chr1:19758800-19758819 MsG0180001348.01.T01:intron 25.0%
! TTTAACATAAGTAATCCTAC+CGG + Chr1:19759691-19759710 None:intergenic 25.0%
!! ATAGGTCATTTAAGTTTCTA+AGG + Chr1:19758560-19758579 None:intergenic 25.0%
!! GATTGTGATTTGTAATTGAA+AGG + Chr1:19757690-19757709 None:intergenic 25.0%
!! TCATAATATTGCACTTTTAC+AGG + Chr1:19759859-19759878 None:intergenic 25.0%
!!! AAGTTTCTAAGGTTTTAGAT+AGG + Chr1:19758549-19758568 None:intergenic 25.0%
!!! ATGTAACCTTTGAAACTTAA+AGG - Chr1:19758580-19758599 MsG0180001348.01.T01:intron 25.0%
AAGTTTGAAGGAAAAACCTT+TGG + Chr1:19759029-19759048 None:intergenic 30.0%
AATTCCTTTGAACAATGACA+AGG - Chr1:19759119-19759138 MsG0180001348.01.T01:intron 30.0%
AGTCTAAGAATTACTGGATT+TGG + Chr1:19757856-19757875 None:intergenic 30.0%
ATAACCTTGTCATTGTTCAA+AGG + Chr1:19759126-19759145 None:intergenic 30.0%
ATAAGGATATGTAATGCAAC+AGG + Chr1:19757765-19757784 None:intergenic 30.0%
ATAGTTATGGTGAATGAAAG+TGG + Chr1:19758998-19759017 None:intergenic 30.0%
ATTAATCAGCAAAGAATCTC+TGG - Chr1:19758183-19758202 MsG0180001348.01.T01:intron 30.0%
ATTTGTAATATATGAGGGAG+AGG + Chr1:19759427-19759446 None:intergenic 30.0%
CGAAATCATGAACTACATAA+GGG - Chr1:19757590-19757609 MsG0180001348.01.T01:CDS 30.0%
GATATTTGGTCACAATACAT+TGG - Chr1:19758023-19758042 MsG0180001348.01.T01:intron 30.0%
GTATCCTCTATTTCAATTCA+AGG + Chr1:19758760-19758779 None:intergenic 30.0%
GTTACAAACGTGAAACTTAA+AGG - Chr1:19758611-19758630 MsG0180001348.01.T01:intron 30.0%
TCGAAATCATGAACTACATA+AGG - Chr1:19757589-19757608 MsG0180001348.01.T01:CDS 30.0%
TTAACATAAGTAATCCTACC+GGG + Chr1:19759690-19759709 None:intergenic 30.0%
TTCATAGATGAAAGTTGTGA+AGG - Chr1:19758069-19758088 MsG0180001348.01.T01:intron 30.0%
TTGTCATTGTTCAAAGGAAT+TGG + Chr1:19759120-19759139 None:intergenic 30.0%
! ATAGGTCCTTTAAGTTTCAA+AGG + Chr1:19758589-19758608 None:intergenic 30.0%
! GACAATTCAAGTTCAAAACT+TGG + Chr1:19758403-19758422 None:intergenic 30.0%
! GAGGTATTCTACCTTATAAA+TGG - Chr1:19758271-19758290 MsG0180001348.01.T01:intron 30.0%
! TGCTCAACAGGAAATATTTT+GGG - Chr1:19757885-19757904 MsG0180001348.01.T01:CDS 30.0%
! TTTGCAAAGTCTCTAAAAGA+TGG - Chr1:19757952-19757971 MsG0180001348.01.T01:intron 30.0%
!!! TGTTTTTTAACTTTGCAGGT+AGG - Chr1:19758308-19758327 MsG0180001348.01.T01:intron 30.0%
AAATTCGTGCAGTTCACAAA+TGG - Chr1:19759820-19759839 MsG0180001348.01.T01:intron 35.0%
AATTCGTGCAGTTCACAAAT+GGG - Chr1:19759821-19759840 MsG0180001348.01.T01:intron 35.0%
ACATCTCAAAGGAGAAAAAG+TGG - Chr1:19758150-19758169 MsG0180001348.01.T01:intron 35.0%
ACTATCTTCAAGAACTCTTC+TGG + Chr1:19757569-19757588 None:intergenic 35.0%
CATATATACTCGAGAGATCA+TGG + Chr1:19759217-19759236 None:intergenic 35.0%
GAACAATGACAAGGTTATGA+AGG - Chr1:19759128-19759147 MsG0180001348.01.T01:CDS 35.0%
GTAATTCTTAGACTCCCAAA+TGG - Chr1:19757862-19757881 MsG0180001348.01.T01:intron 35.0%
GTTTCACGTTTGTAACAGAT+AGG + Chr1:19758607-19758626 None:intergenic 35.0%
TATGCTCTTGAAAAGTCACT+TGG + Chr1:19758782-19758801 None:intergenic 35.0%
TCATCTGCAAGCATTAGATT+TGG - Chr1:19758824-19758843 MsG0180001348.01.T01:intron 35.0%
TTTGGGAGTCTAAGAATTAC+TGG + Chr1:19757862-19757881 None:intergenic 35.0%
! ACAAAGGAGGCATAATTAAG+AGG - Chr1:19758332-19758351 MsG0180001348.01.T01:intron 35.0%
! CAAAGGAGGCATAATTAAGA+GGG - Chr1:19758333-19758352 MsG0180001348.01.T01:intron 35.0%
! GTGCTCAACAGGAAATATTT+TGG - Chr1:19757884-19757903 MsG0180001348.01.T01:CDS 35.0%
! TGCAGGTTGGCATAAATTTA+AGG - Chr1:19759897-19759916 MsG0180001348.01.T01:intron 35.0%
! TTACGCTCATGGCTATATTT+TGG - Chr1:19758429-19758448 MsG0180001348.01.T01:intron 35.0%
! TTTAGGTGGCCCATATTTTA+AGG - Chr1:19757996-19758015 MsG0180001348.01.T01:intron 35.0%
!! AAGAGACATAAAGAGTGTCA+TGG - Chr1:19759796-19759815 MsG0180001348.01.T01:intron 35.0%
!! GTTTCGAGTTTGTTTCAGAT+AGG - Chr1:19758497-19758516 MsG0180001348.01.T01:intron 35.0%
!! TCAACAGGAAATATTTTGGG+TGG - Chr1:19757888-19757907 MsG0180001348.01.T01:CDS 35.0%
!!! GGTTTTAGATAGGTCCTTAA+CGG + Chr1:19758539-19758558 None:intergenic 35.0%
AACTTCACCTCCACTTTCAT+AGG + Chr1:19758860-19758879 None:intergenic 40.0%
AACTTTGCAGGTAGGAACAA+AGG - Chr1:19758316-19758335 MsG0180001348.01.T01:intron 40.0%
AATAGGCCAAATACCTTCCT+GGG + Chr1:19758638-19758657 None:intergenic 40.0%
ACACGAATCTCGATTGTGTT+CGG + Chr1:19759655-19759674 None:intergenic 40.0%
AGAACTTGTAGGTTCTATGC+AGG - Chr1:19759880-19759899 MsG0180001348.01.T01:intron 40.0%
ATAACTATCACCCAAGCCAA+AGG - Chr1:19759010-19759029 MsG0180001348.01.T01:intron 40.0%
ATTCGTGCAGTTCACAAATG+GGG - Chr1:19759822-19759841 MsG0180001348.01.T01:intron 40.0%
ATTTCCTGTTGAGCACCATT+TGG + Chr1:19757880-19757899 None:intergenic 40.0%
CTTTCTATCAACCTCCGTTA+AGG - Chr1:19758522-19758541 MsG0180001348.01.T01:intron 40.0%
GAAGTTGTTGAACTGAGTGA+TGG - Chr1:19758108-19758127 MsG0180001348.01.T01:intron 40.0%
GAGAGTTCCCAAGAAATTTG+TGG - Chr1:19757810-19757829 MsG0180001348.01.T01:intron 40.0%
GATGGCAATGTTAATAGTGC+TGG - Chr1:19758126-19758145 MsG0180001348.01.T01:intron 40.0%
TAATAGGCCAAATACCTTCC+TGG + Chr1:19758639-19758658 None:intergenic 40.0%
TCACAAATGGGGGAAAATCA+TGG - Chr1:19759833-19759852 MsG0180001348.01.T01:intron 40.0%
TGAAGGAAAAACCTTTGGCT+TGG + Chr1:19759024-19759043 None:intergenic 40.0%
TGAGAACATGCCTATGAAAG+TGG - Chr1:19758847-19758866 MsG0180001348.01.T01:intron 40.0%
TGTGTTTCAGCAGACTTAGA+AGG - Chr1:19759613-19759632 MsG0180001348.01.T01:CDS 40.0%
TTGTCACAATCTTACGCTCA+TGG - Chr1:19758418-19758437 MsG0180001348.01.T01:intron 40.0%
TTTAGATAGGTCCTTAACGG+AGG + Chr1:19758536-19758555 None:intergenic 40.0%
TTTCCTGTTGAGCACCATTT+GGG + Chr1:19757879-19757898 None:intergenic 40.0%
! GAAGATTGCGGATTGAAGAT+TGG - Chr1:19759919-19759938 MsG0180001348.01.T01:intron 40.0%
! GAAGGAAAAACCTTTGGCTT+GGG + Chr1:19759023-19759042 None:intergenic 40.0%
!! CTTAACGGAGGTTGATAGAA+AGG + Chr1:19758524-19758543 None:intergenic 40.0%
!! TGGAAGAAAAGAGGCATTGA+AGG - Chr1:19759176-19759195 MsG0180001348.01.T01:CDS 40.0%
!! TTTGGCTTGGGTGATAGTTA+TGG + Chr1:19759011-19759030 None:intergenic 40.0%
ACGTGAAACTTAAAGGACCC+AGG - Chr1:19758618-19758637 MsG0180001348.01.T01:intron 45.0%
AGAACATGCATTGTCACCTC+TGG - Chr1:19758899-19758918 MsG0180001348.01.T01:intron 45.0%
CTTGTAGGTTCTATGCAGGT+TGG - Chr1:19759884-19759903 MsG0180001348.01.T01:intron 45.0%
GAAACTTAAAGGACCCAGGA+AGG - Chr1:19758622-19758641 MsG0180001348.01.T01:intron 45.0%
GAACATGCCTATGAAAGTGG+AGG - Chr1:19758850-19758869 MsG0180001348.01.T01:intron 45.0%
GTTTCAGCAGACTTAGAAGG+TGG - Chr1:19759616-19759635 MsG0180001348.01.T01:CDS 45.0%
TCGTGTGAACTCTACTTACC+CGG - Chr1:19759669-19759688 MsG0180001348.01.T01:CDS 45.0%
TGCTTGCAGATGACCCTTTA+AGG + Chr1:19758817-19758836 None:intergenic 45.0%
TTCGTGCAGTTCACAAATGG+GGG - Chr1:19759823-19759842 MsG0180001348.01.T01:intron 45.0%
TTTGCAGGTAGGAACAAAGG+AGG - Chr1:19758319-19758338 MsG0180001348.01.T01:intron 45.0%
! ACTTAGAAGGTGGTAATGCG+AGG - Chr1:19759626-19759645 MsG0180001348.01.T01:CDS 45.0%
! CTTAGAAGGTGGTAATGCGA+GGG - Chr1:19759627-19759646 MsG0180001348.01.T01:CDS 45.0%
!! AAAGGACCCAGGAAGGTATT+TGG - Chr1:19758629-19758648 MsG0180001348.01.T01:intron 45.0%
!! ATAGTGCTGGCACATCTCAA+AGG - Chr1:19758139-19758158 MsG0180001348.01.T01:intron 45.0%
!! ATATTTTGGGTGGAGCGTGA+CGG - Chr1:19757898-19757917 MsG0180001348.01.T01:CDS 45.0%
!! GAGGCATTGAAGGAAGTTCA+AGG - Chr1:19759186-19759205 MsG0180001348.01.T01:CDS 45.0%
ACTCCCAAATGGTGCTCAAC+AGG - Chr1:19757873-19757892 MsG0180001348.01.T01:intron 50.0%
CATGCATTGTCACCTCTGGT+TGG - Chr1:19758903-19758922 MsG0180001348.01.T01:intron 50.0%
GTGAACTCTACTTACCCGGT+AGG - Chr1:19759673-19759692 MsG0180001348.01.T01:CDS 50.0%
TGGAGCGTGACGGTGATATT+TGG - Chr1:19757908-19757927 MsG0180001348.01.T01:CDS 50.0%
GGCAAATGGCTTCCAACCAG+AGG + Chr1:19758918-19758937 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 19757551 19760020 19757551 ID=MsG0180001348.01;Name=MsG0180001348.01
Chr1 mRNA 19757551 19760020 19757551 ID=MsG0180001348.01.T01;Parent=MsG0180001348.01;Name=MsG0180001348.01.T01;_AED=0.27;_eAED=0.27;_QI=0|0|0|1|0.8|0.83|6|0|348
Chr1 exon 19759952 19760020 19759952 ID=MsG0180001348.01.T01:exon:14489;Parent=MsG0180001348.01.T01
Chr1 exon 19759367 19759760 19759367 ID=MsG0180001348.01.T01:exon:14488;Parent=MsG0180001348.01.T01
Chr1 exon 19759121 19759245 19759121 ID=MsG0180001348.01.T01:exon:14487;Parent=MsG0180001348.01.T01
Chr1 exon 19758651 19758798 19758651 ID=MsG0180001348.01.T01:exon:14486;Parent=MsG0180001348.01.T01
Chr1 exon 19757881 19757945 19757881 ID=MsG0180001348.01.T01:exon:14485;Parent=MsG0180001348.01.T01
Chr1 exon 19757551 19757796 19757551 ID=MsG0180001348.01.T01:exon:14484;Parent=MsG0180001348.01.T01
Chr1 CDS 19759952 19760020 19759952 ID=MsG0180001348.01.T01:cds;Parent=MsG0180001348.01.T01
Chr1 CDS 19759367 19759760 19759367 ID=MsG0180001348.01.T01:cds;Parent=MsG0180001348.01.T01
Chr1 CDS 19759121 19759245 19759121 ID=MsG0180001348.01.T01:cds;Parent=MsG0180001348.01.T01
Chr1 CDS 19758651 19758798 19758651 ID=MsG0180001348.01.T01:cds;Parent=MsG0180001348.01.T01
Chr1 CDS 19757881 19757945 19757881 ID=MsG0180001348.01.T01:cds;Parent=MsG0180001348.01.T01
Chr1 CDS 19757551 19757796 19757551 ID=MsG0180001348.01.T01:cds;Parent=MsG0180001348.01.T01
Gene Sequence

>MsG0180001348.01.T01

ATGTCATTACATCATCCAGAAGAGTTCTTGAAGATAGTTCGAAATCATGAACTACATAAGGGAGAGCTTAGAGTTCCCAAGAAATTTGTGGAGAAATATTGGAAAAGAATACCAAATCCAGTAATTCTTAGACTCCCAAATGGTGCTCAACAGGAAATATTTTGGGTGGAGCGTGACGGTGATATTTGGTTTAAGAGAAATTGGGAAAAATTTGCAAAGTCTCTAAAAGATGGATATGCTTTAGTTTTCAAATATTTAGGTGGCCCATATTTTAAGGTTAAGATATTTGGTCACAATACATTGGAAAAAGACTATTCCAATATAAAATTCATAGATGAAAGTTGTGAAGGTAGAGAAGAAGTTCAAGAAGTTGTTGAACTGAGTGATGGCAATGTTAATAGTGCTGGCACATCTCAAAGGAGAAAAAGTGGCAAGAGAAAAATTAATCAGCAAAGAATCTCTGGTAGGAACAAAGGAGGCATAATTAAGAGGGCTAAAAAATGCTCAACAATTAGTGAGAGAGACAAGAATGAGAATCCAAGTTTTGAACTTGAATTGTCACAATCTTACGCTCATGGCTATATTTTGAGGATACCAAGTGACTTTTCAAGAGCATATTTGAATGACAACCTTAAAGGGTCATCTGCAAGCATTAGATTTGGTGAGAACATGCCTATGAAAGTGGAGGTGAAGTTTCAAGATATCAAGAATAATAGAACATGCATTGTCACCTCTGACTTAGAAGGTGGTAATGCGAGGGAACCGAACACAATCGAGATTCGTGTGAACTCTACTTACCCGTACATTAAACATGAGTTTCTTAAGAGACATAAAGAGTGTCATGGAAAATTCGTGCAGTTCACAAATGGGGGAAAATCATGGCCTGTAAAAGTGCAATATTATGAGAGAACTTGTAGGTTCTATGCAGGTTGGCATAAATTTAAGGAAGATTGCGGATTGAAGATTGGAGATATTTGCAAATTGAAGTTGATTGATGAAAAAATGTTTGTGTTTAAAGTTTCAATTGAAAGAAAGCACCACCACTAG
Protein sequence

>MsG0180001348.01.T01

MSLHHPEEFLKIVRNHELHKGELRVPKKFVEKYWKRIPNPVILRLPNGAQQEIFWVERDGDIWFKRNWEKFAKSLKDGYALVFKYLGGPYFKVKIFGHNTLEKDYSNIKFIDESCEGREEVQEVVELSDGNVNSAGTSQRRKSGKRKINQQRISGRNKGGIIKRAKKCSTISERDKNENPSFELELSQSYAHGYILRIPSDFSRAYLNDNLKGSSASIRFGENMPMKVEVKFQDIKNNRTCIVTSDLEGGNAREPNTIEIRVNSTYPYIKHEFLKRHKECHGKFVQFTNGGKSWPVKVQYYERTCRFYAGWHKFKEDCGLKIGDICKLKLIDEKMFVFKVSIERKHHH*