AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001549.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001549.01.T01 MTR_1g038400 93.827 567 35 0 1 567 1 567 0.0 1097
MsG0180001549.01.T01 MTR_1g038370 72.069 580 114 4 22 575 2 559 0.0 847
MsG0180001549.01.T01 MTR_5g029370 56.379 486 211 1 10 495 71 555 0.0 593
MsG0180001549.01.T01 MTR_5g029370 56.379 486 211 1 10 495 71 555 0.0 593
MsG0180001549.01.T01 MTR_7g407000 79.167 144 12 2 201 343 28 154 2.85e-74 233
MsG0180001549.01.T01 MTR_3g075210 37.602 367 193 8 21 366 11 362 7.61e-67 236
MsG0180001549.01.T01 MTR_8g089260 37.209 387 170 9 24 345 29 407 1.42e-57 208
MsG0180001549.01.T01 MTR_1g078070 30.639 532 272 16 25 475 147 662 3.53e-54 199
MsG0180001549.01.T01 MTR_1g078070 30.639 532 272 16 25 475 147 662 4.90e-54 198
MsG0180001549.01.T01 MTR_5g010300 34.243 403 224 14 133 512 327 711 3.63e-53 196
MsG0180001549.01.T01 MTR_5g010300 34.243 403 224 14 133 512 327 711 3.99e-53 196
MsG0180001549.01.T01 MTR_5g010300 34.243 403 224 14 133 512 327 711 4.14e-53 196
MsG0180001549.01.T01 MTR_2g047130 35.149 404 183 12 24 359 48 440 3.73e-52 192
MsG0180001549.01.T01 MTR_2g047130 35.149 404 183 12 24 359 45 437 3.77e-52 192
MsG0180001549.01.T01 MTR_5g010300 34.521 365 202 12 107 449 302 651 4.64e-50 187
MsG0180001549.01.T01 MTR_1g094740 43.850 187 101 1 173 359 292 474 5.96e-50 186
MsG0180001549.01.T01 MTR_7g097070 25.995 427 283 12 176 576 338 757 1.06e-29 125
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001549.01.T01 AT5G46910 53.909 486 223 1 2 487 77 561 0.0 573
MsG0180001549.01.T01 AT5G46910 53.909 486 223 1 2 487 77 561 0.0 572
MsG0180001549.01.T01 AT2G34880 31.879 527 278 18 25 483 58 571 2.98e-59 211
MsG0180001549.01.T01 AT3G48430 33.947 380 209 7 17 359 9 383 8.88e-59 213
MsG0180001549.01.T01 AT1G63490 42.066 271 131 6 107 360 46 307 2.25e-57 208
MsG0180001549.01.T01 AT1G63490 42.066 271 131 6 107 360 299 560 2.71e-57 208
MsG0180001549.01.T01 AT1G63490 42.066 271 131 6 107 360 299 560 2.81e-57 208
MsG0180001549.01.T01 AT1G30810 30.798 539 277 16 24 483 55 576 2.02e-56 203
MsG0180001549.01.T01 AT1G30810 30.798 539 277 16 24 483 55 576 2.02e-56 203
MsG0180001549.01.T01 AT1G30810 30.798 539 277 16 24 483 55 576 2.02e-56 203
MsG0180001549.01.T01 AT4G20400 28.462 520 288 14 24 468 52 562 1.14e-53 197
MsG0180001549.01.T01 AT1G08620 33.810 420 192 12 25 369 157 565 3.36e-52 193
MsG0180001549.01.T01 AT1G08620 33.810 420 192 12 25 369 143 551 4.36e-52 193
MsG0180001549.01.T01 AT1G08620 33.810 420 192 12 25 369 143 551 4.36e-52 193
MsG0180001549.01.T01 AT1G08620 33.810 420 192 12 25 369 143 551 4.36e-52 193
MsG0180001549.01.T01 AT5G04240 46.277 188 97 1 172 359 259 442 7.30e-51 189
MsG0180001549.01.T01 AT4G20400 28.937 508 277 14 36 468 7 505 2.06e-50 187
MsG0180001549.01.T01 AT2G38950 27.126 494 287 17 22 460 102 577 4.51e-34 138
MsG0180001549.01.T01 AT1G63490 51.724 87 42 0 274 360 15 101 3.91e-24 108

Find 91 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCCATTGTACAGTCTTTC+TGG 0.128715 1:+23119013 MsG0180001549.01.T01:CDS
TTGATTTGGATGATCTAAAT+TGG 0.198966 1:+23116683 MsG0180001549.01.T01:CDS
TGTCTTAATGAAAGAGAAAA+AGG 0.225632 1:+23117060 MsG0180001549.01.T01:CDS
TTCTGAACTGCAAAATTTGT+TGG 0.268593 1:-23117297 None:intergenic
GCTCCTGAGGCTTCTAAATA+TGG 0.273176 1:+23116787 MsG0180001549.01.T01:CDS
TTGGTGATTGGTTTCCATTT+GGG 0.283394 1:+23118845 MsG0180001549.01.T01:CDS
CAAATCGAGTAAATTTGATT+TGG 0.293057 1:+23116669 MsG0180001549.01.T01:CDS
AAACAATTGTACAGCCTCTT+AGG 0.293247 1:+23117095 MsG0180001549.01.T01:CDS
TCCTGCAATATCTTCTGTTT+TGG 0.293249 1:+23119851 MsG0180001549.01.T01:CDS
AATCCAGCATGATATGAATT+AGG 0.298533 1:-23118248 None:intergenic
AGTTCCTCATATGGAATTAT+AGG 0.316333 1:-23118907 None:intergenic
GAGAAGGGAACAGTTGAGTA+TGG 0.324983 1:+23117374 MsG0180001549.01.T01:CDS
AGATCTTCAAATACATCATT+AGG 0.331595 1:+23119096 MsG0180001549.01.T01:CDS
ATAAAGGGTTCGCGGTCTTT+TGG 0.338413 1:-23119790 None:intergenic
ATTGGTGATTGGTTTCCATT+TGG 0.339040 1:+23118844 MsG0180001549.01.T01:CDS
TTACAAAGAAGTTCCTCATA+TGG 0.344918 1:-23118916 None:intergenic
GGATGGTAAACTGGACATTC+TGG 0.345953 1:-23116718 None:intergenic
GAAGGATTAGAGGCACTAAT+TGG 0.346599 1:-23117031 None:intergenic
TTACCATGGAAAAGGCAAAT+TGG 0.347643 1:-23119192 None:intergenic
TGTTCTGATGGATGGTAAAC+TGG 0.349116 1:-23116727 None:intergenic
TATATCCTTCTCATTCCATT+TGG 0.357030 1:-23117122 None:intergenic
CTCATTCCAAGTTCATCATC+AGG 0.362352 1:-23117431 None:intergenic
GACAAAATCAGAAGGATTAG+AGG 0.370940 1:-23117041 None:intergenic
TAAACTTTGCTATTGGTGAT+TGG 0.380389 1:+23118833 MsG0180001549.01.T01:CDS
AATGAGCAAATTCAATTTGA+AGG 0.382800 1:+23117448 MsG0180001549.01.T01:CDS
GAAGAACTGTCAACTCTGTA+AGG 0.420264 1:+23119649 MsG0180001549.01.T01:CDS
ACTGCAAAATTTGTTGGAAA+AGG 0.420420 1:-23117291 None:intergenic
ACAAGAATTTGAGCATCCTT+TGG 0.422698 1:+23116747 MsG0180001549.01.T01:CDS
TCATGTTGTAACAAGACATT+AGG 0.439737 1:-23118173 None:intergenic
TTGCTTGCTGCAGCCCCAAA+TGG 0.446449 1:-23118859 None:intergenic
GAAGCTGTAAACTTTGCTAT+TGG 0.453482 1:+23118826 MsG0180001549.01.T01:CDS
TTACCTTCCTCTCTTAGAAA+AGG 0.456944 1:-23117149 None:intergenic
TGCTCAAATTCTTGTTCTGA+TGG 0.458499 1:-23116739 None:intergenic
GCTACCTATAATTCCATATG+AGG 0.458631 1:+23118903 MsG0180001549.01.T01:CDS
ACTGAAAGCAGAATGATAAT+CGG 0.460027 1:+23119607 MsG0180001549.01.T01:intron
CTGATCCTATGCTTTACATT+GGG 0.461409 1:+23117841 MsG0180001549.01.T01:CDS
TTCCATTTGGAAAGCCTAAG+AGG 0.464543 1:-23117109 None:intergenic
TACAAGGCGGTGCAAAAGCC+AGG 0.481676 1:+23118206 MsG0180001549.01.T01:CDS
TTTCCTAATTCATATCATGC+TGG 0.481919 1:+23118245 MsG0180001549.01.T01:CDS
ACTGATCCTATGCTTTACAT+TGG 0.488180 1:+23117840 MsG0180001549.01.T01:CDS
ACATGATGTGCCAGTTTACA+AGG 0.492030 1:+23118190 MsG0180001549.01.T01:CDS
ATTTACATATCAAGAATTTG+AGG 0.498743 1:+23117253 MsG0180001549.01.T01:CDS
TGACATGCAGCCTCTGCCAT+AGG 0.501176 1:+23119124 MsG0180001549.01.T01:CDS
ACCAAAACAGAAGATATTGC+AGG 0.502442 1:-23119852 None:intergenic
AATTGAGAGGCTGCAGAGCT+AGG 0.502653 1:-23118047 None:intergenic
AGCCTCTTAGGCTTTCCAAA+TGG 0.506480 1:+23117107 MsG0180001549.01.T01:CDS
GGTGGAAATAAAACTTGGTA+TGG 0.514584 1:+23118020 MsG0180001549.01.T01:CDS
CAAATTCTTGTTCTGATGGA+TGG 0.515818 1:-23116735 None:intergenic
TGGTGATTGGTTTCCATTTG+GGG 0.516058 1:+23118846 MsG0180001549.01.T01:CDS
TGCTATTCAGTATGTTTGCA+TGG 0.518171 1:+23117865 MsG0180001549.01.T01:CDS
TAGAAGATCACTATTTGTAC+AGG 0.521776 1:+23117892 MsG0180001549.01.T01:CDS
TGTGATCCTGATGATGAACT+TGG 0.525005 1:+23117425 MsG0180001549.01.T01:CDS
CGAAATGATACATGGAGAGA+AGG 0.529280 1:+23117358 MsG0180001549.01.T01:CDS
ACTCAGGTGGAAATAAAACT+TGG 0.530514 1:+23118015 MsG0180001549.01.T01:CDS
CTCAAATTATAAAGGGTTCG+CGG 0.531379 1:-23119798 None:intergenic
TGTTGTAACAAGACATTAGG+AGG 0.534027 1:-23118170 None:intergenic
TTCATTAAGACAAAATCAGA+AGG 0.538081 1:-23117049 None:intergenic
TTAGCAGAACATGGAGAGAA+TGG 0.538483 1:+23118110 MsG0180001549.01.T01:CDS
TCTACAGAAAATTGCTCCTG+AGG 0.540615 1:+23116774 MsG0180001549.01.T01:CDS
ATACCATATTTAGAAGCCTC+AGG 0.547248 1:-23116790 None:intergenic
AAGCTACATAACAATCCCTA+TGG 0.550057 1:-23119140 None:intergenic
AAGGTGATGACAAACTCTCC+TGG 0.552268 1:-23118224 None:intergenic
GAGTATGGAGTCAACATTGA+GGG 0.553863 1:+23117389 MsG0180001549.01.T01:CDS
ATGGAAAAGGCAAATTGGAT+AGG 0.554570 1:-23119187 None:intergenic
GACATGCAGCCTCTGCCATA+GGG 0.557662 1:+23119125 MsG0180001549.01.T01:CDS
ATAAATTATCATCACTCAGG+TGG 0.565750 1:+23118002 MsG0180001549.01.T01:CDS
CAAGACATTAGGAGGGAACA+TGG 0.568622 1:-23118162 None:intergenic
TTCTGGCACGAAATGATACA+TGG 0.573038 1:+23117350 MsG0180001549.01.T01:CDS
TACTTCTCCATCAGTGATGA+GGG 0.579966 1:+23119767 MsG0180001549.01.T01:CDS
GAAATGATACATGGAGAGAA+GGG 0.582021 1:+23117359 MsG0180001549.01.T01:CDS
CATAACAATCCCTATGGCAG+AGG 0.587190 1:-23119134 None:intergenic
CAAAAGCAAACTCGAAGCCA+TGG 0.587402 1:+23118981 MsG0180001549.01.T01:CDS
TATCATGCTGGATTTAGTCA+TGG 0.587724 1:+23118257 MsG0180001549.01.T01:CDS
AAGAACTGTCAACTCTGTAA+GGG 0.595848 1:+23119650 MsG0180001549.01.T01:CDS
AACAAAATCTTAGCAGAACA+TGG 0.596081 1:+23118101 MsG0180001549.01.T01:CDS
AGTATAAATTATCATCACTC+AGG 0.598943 1:+23117999 MsG0180001549.01.T01:intron
ATACTTCTCCATCAGTGATG+AGG 0.601559 1:+23119766 MsG0180001549.01.T01:CDS
GCTTTCCAAATGGAATGAGA+AGG 0.605970 1:+23117117 MsG0180001549.01.T01:CDS
ATTTGAGGCTATAGCAAACA+AGG 0.606737 1:+23117268 MsG0180001549.01.T01:CDS
AATGGCACAATAAGATGCCA+TGG 0.612133 1:-23118998 None:intergenic
AGCATCCCAATGTAAAGCAT+AGG 0.618659 1:-23117846 None:intergenic
TAAACTGAAAACAAGAGTCA+CGG 0.629474 1:+23119683 MsG0180001549.01.T01:CDS
CCTCTGTCAACTAAACGCAA+TGG 0.632529 1:-23117696 None:intergenic
CCATTGCGTTTAGTTGACAG+AGG 0.635713 1:+23117696 MsG0180001549.01.T01:CDS
AGCATGATATGAATTAGGAA+AGG 0.636839 1:-23118243 None:intergenic
TGAGTATGGAGTCAACATTG+AGG 0.665976 1:+23117388 MsG0180001549.01.T01:CDS
TAAAGAATCAGAAGATGACA+AGG 0.671164 1:+23119727 MsG0180001549.01.T01:CDS
GTTGTAACAAGACATTAGGA+GGG 0.697642 1:-23118169 None:intergenic
ACTAAACGCAATGGAGACTG+CGG 0.718204 1:-23117687 None:intergenic
ATGCCAGAAAGACTGTACAA+TGG 0.727660 1:-23119016 None:intergenic
TGATGTGCCAGTTTACAAGG+CGG 0.788729 1:+23118193 MsG0180001549.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCTGATTAATTTTTAATTT+TGG + Chr1:23119576-23119595 MsG0180001549.01.T01:intron 10.0%
!! AATATGTTAATAGAGAAAAA+TGG - Chr1:23117502-23117521 None:intergenic 15.0%
!! AATTTATACTGCAAAAAATA+CGG - Chr1:23117989-23118008 None:intergenic 15.0%
!! GATGAAAAAATAAACTAATT+TGG + Chr1:23117205-23117224 MsG0180001549.01.T01:intron 15.0%
!! TTTATTCTTGATAGATAAAT+TGG + Chr1:23119255-23119274 MsG0180001549.01.T01:intron 15.0%
!!! AATTCTGCTTTAAAATTTTA+GGG + Chr1:23117813-23117832 MsG0180001549.01.T01:intron 15.0%
!!! AGTTTATTTTTTCATCATTT+AGG - Chr1:23117201-23117220 None:intergenic 15.0%
!!! ATGTTTTCTCAAATTATAAA+GGG - Chr1:23119808-23119827 None:intergenic 15.0%
!!! TAATTCTGCTTTAAAATTTT+AGG + Chr1:23117812-23117831 MsG0180001549.01.T01:intron 15.0%
!!! TTATTTATTTTGCATATTTC+AGG + Chr1:23116989-23117008 MsG0180001549.01.T01:intron 15.0%
!!! TTTCATTTGATTTTAGAATT+TGG + Chr1:23117656-23117675 MsG0180001549.01.T01:intron 15.0%
!! AATTATCAAATAGACTAATG+AGG - Chr1:23119220-23119239 None:intergenic 20.0%
!! AATTGATGTAACATTTAGTA+TGG + Chr1:23116917-23116936 MsG0180001549.01.T01:intron 20.0%
!! ATTTACATATCAAGAATTTG+AGG + Chr1:23117253-23117272 MsG0180001549.01.T01:CDS 20.0%
!!! ACATAAGTATTTGAAATATC+AGG - Chr1:23118698-23118717 None:intergenic 20.0%
!!! CATGTTTTCTCAAATTATAA+AGG - Chr1:23119809-23119828 None:intergenic 20.0%
!!! TTCTTGATATGTAAATTTTC+TGG - Chr1:23117249-23117268 None:intergenic 20.0%
!!! TTGAAGATTTTTAGAATCAT+TGG - Chr1:23117552-23117571 None:intergenic 20.0%
!!! TTTATTTTCTTGTTGAGTTT+AGG + Chr1:23118790-23118809 MsG0180001549.01.T01:intron 20.0%
! AATGAGCAAATTCAATTTGA+AGG + Chr1:23117448-23117467 MsG0180001549.01.T01:CDS 25.0%
! AGATAAAAAATCATAAACGC+CGG - Chr1:23116853-23116872 None:intergenic 25.0%
! AGATCTTCAAATACATCATT+AGG + Chr1:23119096-23119115 MsG0180001549.01.T01:CDS 25.0%
! AGTATAAATTATCATCACTC+AGG + Chr1:23117999-23118018 MsG0180001549.01.T01:intron 25.0%
! AGTATGGTTATCAAATATGT+TGG + Chr1:23116933-23116952 MsG0180001549.01.T01:intron 25.0%
! ATAATAACTTAATCCAGACT+AGG - Chr1:23117750-23117769 None:intergenic 25.0%
! CAAATCGAGTAAATTTGATT+TGG + Chr1:23116669-23116688 MsG0180001549.01.T01:CDS 25.0%
! CATGTCATTCTTCTTAAAAA+CGG - Chr1:23119340-23119359 None:intergenic 25.0%
! TGATTTAGTCATGTTTATGT+TGG + Chr1:23118384-23118403 MsG0180001549.01.T01:intron 25.0%
! TGTCTTAATGAAAGAGAAAA+AGG + Chr1:23117060-23117079 MsG0180001549.01.T01:CDS 25.0%
! TTCATTAAGACAAAATCAGA+AGG - Chr1:23117052-23117071 None:intergenic 25.0%
!! ATTTTCTGTAGATAAACCAA+AGG - Chr1:23116766-23116785 None:intergenic 25.0%
!! TAATTGAAAGGTATGATTCA+AGG + Chr1:23118449-23118468 MsG0180001549.01.T01:intron 25.0%
!! TTTACATTTTGCACTAGATA+GGG - Chr1:23118755-23118774 None:intergenic 25.0%
!! TTTTACCTGTTGAAAACTTT+TGG - Chr1:23119382-23119401 None:intergenic 25.0%
!!! ACATCAATTTTTTATGCATG+AGG - Chr1:23116906-23116925 None:intergenic 25.0%
!!! ATAATAACCTTTTCTAAGAG+AGG + Chr1:23117142-23117161 MsG0180001549.01.T01:CDS 25.0%
!!! TTGATTTGGATGATCTAAAT+TGG + Chr1:23116683-23116702 MsG0180001549.01.T01:CDS 25.0%
!!! TTGTTTTTTGTGCAAGAAAT+TGG - Chr1:23118142-23118161 None:intergenic 25.0%
AAAATAAGCCTCGCAAAAAA+TGG - Chr1:23116967-23116986 None:intergenic 30.0%
AAAATCCTAGGCTTGAAAAA+TGG + Chr1:23118515-23118534 MsG0180001549.01.T01:intron 30.0%
AACAAAATCTTAGCAGAACA+TGG + Chr1:23118101-23118120 MsG0180001549.01.T01:CDS 30.0%
AATCCAGCATGATATGAATT+AGG - Chr1:23118251-23118270 None:intergenic 30.0%
ACAAGTAATCTGAAAATCCT+AGG + Chr1:23118503-23118522 MsG0180001549.01.T01:intron 30.0%
ACTGAAAGCAGAATGATAAT+CGG + Chr1:23119607-23119626 MsG0180001549.01.T01:intron 30.0%
ACTTATGTTGCATACTTGTT+CGG + Chr1:23118710-23118729 MsG0180001549.01.T01:intron 30.0%
AGCATGATATGAATTAGGAA+AGG - Chr1:23118246-23118265 None:intergenic 30.0%
AGTTCCTCATATGGAATTAT+AGG - Chr1:23118910-23118929 None:intergenic 30.0%
ATAAATTATCATCACTCAGG+TGG + Chr1:23118002-23118021 MsG0180001549.01.T01:CDS 30.0%
CTCTATATGCACAAACTATT+AGG - Chr1:23118637-23118656 None:intergenic 30.0%
GAACAGGCAATATTTGATTA+TGG - Chr1:23118578-23118597 None:intergenic 30.0%
GATAAAAAATCATAAACGCC+GGG - Chr1:23116852-23116871 None:intergenic 30.0%
GTCCAAAAGTAAACAGATTA+CGG - Chr1:23118602-23118621 None:intergenic 30.0%
TAAACTGAAAACAAGAGTCA+CGG + Chr1:23119683-23119702 MsG0180001549.01.T01:CDS 30.0%
TAAAGAATCAGAAGATGACA+AGG + Chr1:23119727-23119746 MsG0180001549.01.T01:CDS 30.0%
TAGAAGATCACTATTTGTAC+AGG + Chr1:23117892-23117911 MsG0180001549.01.T01:CDS 30.0%
TATATCCTTCTCATTCCATT+TGG - Chr1:23117125-23117144 None:intergenic 30.0%
TCAAGGTTAATCTTGTAAAC+TGG + Chr1:23118466-23118485 MsG0180001549.01.T01:intron 30.0%
TCATGTTGTAACAAGACATT+AGG - Chr1:23118176-23118195 None:intergenic 30.0%
TCTAGTGCAAAATGTAAAGA+TGG + Chr1:23118757-23118776 MsG0180001549.01.T01:intron 30.0%
TTACAAAGAAGTTCCTCATA+TGG - Chr1:23118919-23118938 None:intergenic 30.0%
TTCTGAACTGCAAAATTTGT+TGG - Chr1:23117300-23117319 None:intergenic 30.0%
TTTCCTAATTCATATCATGC+TGG + Chr1:23118245-23118264 MsG0180001549.01.T01:CDS 30.0%
! AGATGATTTTCTTTTCATCC+CGG + Chr1:23116831-23116850 MsG0180001549.01.T01:intron 30.0%
! CTTTACATTTTGCACTAGAT+AGG - Chr1:23118756-23118775 None:intergenic 30.0%
! TCCAAAAATGGTTTTCTTAC+AGG - Chr1:23117726-23117745 None:intergenic 30.0%
! TCCTGTAAGAAAACCATTTT+TGG + Chr1:23117722-23117741 MsG0180001549.01.T01:intron 30.0%
! TTTGTAACTTCTGACTTTTG+AGG - Chr1:23118965-23118984 None:intergenic 30.0%
!! ACTGCAAAATTTGTTGGAAA+AGG - Chr1:23117294-23117313 None:intergenic 30.0%
!! TAAACTTTGCTATTGGTGAT+TGG + Chr1:23118833-23118852 MsG0180001549.01.T01:CDS 30.0%
!! TAGATATCGAAAAAGCATTC+TGG + Chr1:23117333-23117352 MsG0180001549.01.T01:CDS 30.0%
!!! CATTTTTTCCATTTTTTGCG+AGG + Chr1:23116956-23116975 MsG0180001549.01.T01:intron 30.0%
!!! TTGAGTTTAGGTTTTAACTG+TGG + Chr1:23118802-23118821 MsG0180001549.01.T01:intron 30.0%
!!! TTTGATTTTAGAATTTGGCG+CGG + Chr1:23117661-23117680 MsG0180001549.01.T01:intron 30.0%
AAACAATTGTACAGCCTCTT+AGG + Chr1:23117095-23117114 MsG0180001549.01.T01:CDS 35.0%
AAGAACTGTCAACTCTGTAA+GGG + Chr1:23119650-23119669 MsG0180001549.01.T01:CDS 35.0%
AAGCTACATAACAATCCCTA+TGG - Chr1:23119143-23119162 None:intergenic 35.0%
ACAAGAATTTGAGCATCCTT+TGG + Chr1:23116747-23116766 MsG0180001549.01.T01:CDS 35.0%
ACCAAAACAGAAGATATTGC+AGG - Chr1:23119855-23119874 None:intergenic 35.0%
ACTCAGGTGGAAATAAAACT+TGG + Chr1:23118015-23118034 MsG0180001549.01.T01:CDS 35.0%
ACTGATCCTATGCTTTACAT+TGG + Chr1:23117840-23117859 MsG0180001549.01.T01:CDS 35.0%
AGATCGCTCGAATAATTGAA+AGG + Chr1:23118437-23118456 MsG0180001549.01.T01:intron 35.0%
ATACCATATTTAGAAGCCTC+AGG - Chr1:23116793-23116812 None:intergenic 35.0%
ATGGAAAAGGCAAATTGGAT+AGG - Chr1:23119190-23119209 None:intergenic 35.0%
CAAATTCTTGTTCTGATGGA+TGG - Chr1:23116738-23116757 None:intergenic 35.0%
CTCAAATTATAAAGGGTTCG+CGG - Chr1:23119801-23119820 None:intergenic 35.0%
CTCATAGTTTACGGATAAGT+AGG + Chr1:23118413-23118432 MsG0180001549.01.T01:intron 35.0%
CTGATCCTATGCTTTACATT+GGG + Chr1:23117841-23117860 MsG0180001549.01.T01:CDS 35.0%
GAAATGATACATGGAGAGAA+GGG + Chr1:23117359-23117378 MsG0180001549.01.T01:CDS 35.0%
GACAAAATCAGAAGGATTAG+AGG - Chr1:23117044-23117063 None:intergenic 35.0%
GCTACCTATAATTCCATATG+AGG + Chr1:23118903-23118922 MsG0180001549.01.T01:CDS 35.0%
GGTGGAAATAAAACTTGGTA+TGG + Chr1:23118020-23118039 MsG0180001549.01.T01:CDS 35.0%
GTTGTAACAAGACATTAGGA+GGG - Chr1:23118172-23118191 None:intergenic 35.0%
TATCATGCTGGATTTAGTCA+TGG + Chr1:23118257-23118276 MsG0180001549.01.T01:CDS 35.0%
TGCTATTCAGTATGTTTGCA+TGG + Chr1:23117865-23117884 MsG0180001549.01.T01:CDS 35.0%
TGCTCAAATTCTTGTTCTGA+TGG - Chr1:23116742-23116761 None:intergenic 35.0%
TGGCTATTGCTCATAGTTTA+CGG + Chr1:23118404-23118423 MsG0180001549.01.T01:intron 35.0%
TGTTGTAACAAGACATTAGG+AGG - Chr1:23118173-23118192 None:intergenic 35.0%
TTACCATGGAAAAGGCAAAT+TGG - Chr1:23119195-23119214 None:intergenic 35.0%
TTACCTTCCTCTCTTAGAAA+AGG - Chr1:23117152-23117171 None:intergenic 35.0%
! AGACTAATGAGGTTTTACCA+TGG - Chr1:23119209-23119228 None:intergenic 35.0%
! ATGAGGTTTTACCATGGAAA+AGG - Chr1:23119203-23119222 None:intergenic 35.0%
! ATTTGAGGCTATAGCAAACA+AGG + Chr1:23117268-23117287 MsG0180001549.01.T01:CDS 35.0%
! ATTTTGCACTAGATAGGGAT+AGG - Chr1:23118750-23118769 None:intergenic 35.0%
! CTCCGTAATCTGTTTACTTT+TGG + Chr1:23118597-23118616 MsG0180001549.01.T01:intron 35.0%
! GAAGCTGTAAACTTTGCTAT+TGG + Chr1:23118826-23118845 MsG0180001549.01.T01:CDS 35.0%
! GACAGTCTTTTCAAATTGAG+AGG - Chr1:23118063-23118082 None:intergenic 35.0%
! TATCCAATTTGCCTTTTCCA+TGG + Chr1:23119189-23119208 MsG0180001549.01.T01:CDS 35.0%
! TCCTGCAATATCTTCTGTTT+TGG + Chr1:23119851-23119870 MsG0180001549.01.T01:CDS 35.0%
! TGCCAAAAACTTGCTTATGT+GGG + Chr1:23119304-23119323 MsG0180001549.01.T01:CDS 35.0%
!! AATCTGTTTACTTTTGGACC+AGG + Chr1:23118603-23118622 MsG0180001549.01.T01:intron 35.0%
!! ATCTGTTTACTTTTGGACCA+GGG + Chr1:23118604-23118623 MsG0180001549.01.T01:intron 35.0%
!! ATTGGTGATTGGTTTCCATT+TGG + Chr1:23118844-23118863 MsG0180001549.01.T01:CDS 35.0%
!! TAACCTTTTCTAAGAGAGGA+AGG + Chr1:23117146-23117165 MsG0180001549.01.T01:CDS 35.0%
!! TTGGTGATTGGTTTCCATTT+GGG + Chr1:23118845-23118864 MsG0180001549.01.T01:CDS 35.0%
AATGGCACAATAAGATGCCA+TGG - Chr1:23119001-23119020 None:intergenic 40.0%
ACATGATGTGCCAGTTTACA+AGG + Chr1:23118190-23118209 MsG0180001549.01.T01:CDS 40.0%
AGCATCCCAATGTAAAGCAT+AGG - Chr1:23117849-23117868 None:intergenic 40.0%
ATACTTCTCCATCAGTGATG+AGG + Chr1:23119766-23119785 MsG0180001549.01.T01:CDS 40.0%
ATGCCAGAAAGACTGTACAA+TGG - Chr1:23119019-23119038 None:intergenic 40.0%
CCTAGGCTTGAAAAATGGAA+AGG + Chr1:23118520-23118539 MsG0180001549.01.T01:intron 40.0%
CGAAATGATACATGGAGAGA+AGG + Chr1:23117358-23117377 MsG0180001549.01.T01:CDS 40.0%
CTCATTCCAAGTTCATCATC+AGG - Chr1:23117434-23117453 None:intergenic 40.0%
GAAGAACTGTCAACTCTGTA+AGG + Chr1:23119649-23119668 MsG0180001549.01.T01:CDS 40.0%
GACGCATTCAATCATGCTAT+AGG - Chr1:23119442-23119461 None:intergenic 40.0%
GAGTATGGAGTCAACATTGA+GGG + Chr1:23117389-23117408 MsG0180001549.01.T01:CDS 40.0%
GCTTTCCAAATGGAATGAGA+AGG + Chr1:23117117-23117136 MsG0180001549.01.T01:CDS 40.0%
GTAAACAGATTACGGAGAAC+AGG - Chr1:23118594-23118613 None:intergenic 40.0%
TACTTCTCCATCAGTGATGA+GGG + Chr1:23119767-23119786 MsG0180001549.01.T01:CDS 40.0%
TCCAGACTAGGTTCCAAAAA+TGG - Chr1:23117738-23117757 None:intergenic 40.0%
TCTACAGAAAATTGCTCCTG+AGG + Chr1:23116774-23116793 MsG0180001549.01.T01:CDS 40.0%
TGAGTATGGAGTCAACATTG+AGG + Chr1:23117388-23117407 MsG0180001549.01.T01:CDS 40.0%
TTAGCAGAACATGGAGAGAA+TGG + Chr1:23118110-23118129 MsG0180001549.01.T01:CDS 40.0%
TTCCATTTGGAAAGCCTAAG+AGG - Chr1:23117112-23117131 None:intergenic 40.0%
TTCTGGCACGAAATGATACA+TGG + Chr1:23117350-23117369 MsG0180001549.01.T01:CDS 40.0%
! CCAAAAACTTGCTTATGTGG+GGG + Chr1:23119306-23119325 MsG0180001549.01.T01:CDS 40.0%
! GCCAAAAACTTGCTTATGTG+GGG + Chr1:23119305-23119324 MsG0180001549.01.T01:CDS 40.0%
! GTGCCAAAAACTTGCTTATG+TGG + Chr1:23119303-23119322 MsG0180001549.01.T01:CDS 40.0%
! TGCTGCCAAAAGTTTTCAAC+AGG + Chr1:23119374-23119393 MsG0180001549.01.T01:CDS 40.0%
! TGTGATCCTGATGATGAACT+TGG + Chr1:23117425-23117444 MsG0180001549.01.T01:CDS 40.0%
! TGTTCTGATGGATGGTAAAC+TGG - Chr1:23116730-23116749 None:intergenic 40.0%
!! GAAGGATTAGAGGCACTAAT+TGG - Chr1:23117034-23117053 None:intergenic 40.0%
!! TGGTGATTGGTTTCCATTTG+GGG + Chr1:23118846-23118865 MsG0180001549.01.T01:CDS 40.0%
!! TTATGCATGAGGAGTGTTCT+TGG - Chr1:23116895-23116914 None:intergenic 40.0%
!!! ACCATTTTTGGAACCTAGTC+TGG + Chr1:23117734-23117753 MsG0180001549.01.T01:intron 40.0%
!!! CCTTTCCATTTTTCAAGCCT+AGG - Chr1:23118523-23118542 None:intergenic 40.0%
AACTATTAGGACTTCAGCCC+TGG - Chr1:23118624-23118643 None:intergenic 45.0%
AAGGTGATGACAAACTCTCC+TGG - Chr1:23118227-23118246 None:intergenic 45.0%
ACTAAACGCAATGGAGACTG+CGG - Chr1:23117690-23117709 None:intergenic 45.0%
AGCCTCTTAGGCTTTCCAAA+TGG + Chr1:23117107-23117126 MsG0180001549.01.T01:CDS 45.0%
CAAAAGCAAACTCGAAGCCA+TGG + Chr1:23118981-23119000 MsG0180001549.01.T01:CDS 45.0%
CAAGACATTAGGAGGGAACA+TGG - Chr1:23118165-23118184 None:intergenic 45.0%
CATAACAATCCCTATGGCAG+AGG - Chr1:23119137-23119156 None:intergenic 45.0%
CCATTGCGTTTAGTTGACAG+AGG + Chr1:23117696-23117715 MsG0180001549.01.T01:CDS 45.0%
CCTCTGTCAACTAAACGCAA+TGG - Chr1:23117699-23117718 None:intergenic 45.0%
CTTCAGCCATTAGGCACAAT+TGG + Chr1:23118545-23118564 MsG0180001549.01.T01:intron 45.0%
GAGAAGGGAACAGTTGAGTA+TGG + Chr1:23117374-23117393 MsG0180001549.01.T01:CDS 45.0%
GATCGACCAATTGTGCCTAA+TGG - Chr1:23118554-23118573 None:intergenic 45.0%
GCTCCTGAGGCTTCTAAATA+TGG + Chr1:23116787-23116806 MsG0180001549.01.T01:CDS 45.0%
GGATGGTAAACTGGACATTC+TGG - Chr1:23116721-23116740 None:intergenic 45.0%
TGATGTGCCAGTTTACAAGG+CGG + Chr1:23118193-23118212 MsG0180001549.01.T01:CDS 45.0%
! ATAAAGGGTTCGCGGTCTTT+TGG - Chr1:23119793-23119812 None:intergenic 45.0%
! CCCCCACATAAGCAAGTTTT+TGG - Chr1:23119309-23119328 None:intergenic 45.0%
! GTGCCATTGTACAGTCTTTC+TGG + Chr1:23119013-23119032 MsG0180001549.01.T01:CDS 45.0%
! TTTTGCACCGCCTTGTAAAC+TGG - Chr1:23118203-23118222 None:intergenic 45.0%
!! GGAAAGGTACTTCAGCCATT+AGG + Chr1:23118536-23118555 MsG0180001549.01.T01:intron 45.0%
AATTGAGAGGCTGCAGAGCT+AGG - Chr1:23118050-23118069 None:intergenic 50.0%
AGAGAGAGAGAGAGAGAGAG+AGG - Chr1:23118301-23118320 None:intergenic 50.0%
! CTTTTGGCCCTCATCACTGA+TGG - Chr1:23119777-23119796 None:intergenic 50.0%
GACATGCAGCCTCTGCCATA+GGG + Chr1:23119125-23119144 MsG0180001549.01.T01:CDS 55.0%
TACAAGGCGGTGCAAAAGCC+AGG + Chr1:23118206-23118225 MsG0180001549.01.T01:CDS 55.0%
TGACATGCAGCCTCTGCCAT+AGG + Chr1:23119124-23119143 MsG0180001549.01.T01:CDS 55.0%
! TTGCTTGCTGCAGCCCCAAA+TGG - Chr1:23118862-23118881 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 23116643 23119896 23116643 ID=MsG0180001549.01;Name=MsG0180001549.01
Chr1 mRNA 23116643 23119896 23116643 ID=MsG0180001549.01.T01;Parent=MsG0180001549.01;Name=MsG0180001549.01.T01;_AED=0.18;_eAED=0.18;_QI=0|0|0|1|1|0.88|9|0|576
Chr1 exon 23116643 23116808 23116643 ID=MsG0180001549.01.T01:exon:40404;Parent=MsG0180001549.01.T01
Chr1 exon 23117011 23117167 23117011 ID=MsG0180001549.01.T01:exon:40405;Parent=MsG0180001549.01.T01
Chr1 exon 23117250 23117469 23117250 ID=MsG0180001549.01.T01:exon:40406;Parent=MsG0180001549.01.T01
Chr1 exon 23117672 23117725 23117672 ID=MsG0180001549.01.T01:exon:40407;Parent=MsG0180001549.01.T01
Chr1 exon 23117834 23117913 23117834 ID=MsG0180001549.01.T01:exon:40408;Parent=MsG0180001549.01.T01
Chr1 exon 23118001 23118278 23118001 ID=MsG0180001549.01.T01:exon:40409;Parent=MsG0180001549.01.T01
Chr1 exon 23118812 23119210 23118812 ID=MsG0180001549.01.T01:exon:40410;Parent=MsG0180001549.01.T01
Chr1 exon 23119298 23119395 23119298 ID=MsG0180001549.01.T01:exon:40411;Parent=MsG0180001549.01.T01
Chr1 exon 23119618 23119896 23119618 ID=MsG0180001549.01.T01:exon:40412;Parent=MsG0180001549.01.T01
Chr1 CDS 23116643 23116808 23116643 ID=MsG0180001549.01.T01:cds;Parent=MsG0180001549.01.T01
Chr1 CDS 23117011 23117167 23117011 ID=MsG0180001549.01.T01:cds;Parent=MsG0180001549.01.T01
Chr1 CDS 23117250 23117469 23117250 ID=MsG0180001549.01.T01:cds;Parent=MsG0180001549.01.T01
Chr1 CDS 23117672 23117725 23117672 ID=MsG0180001549.01.T01:cds;Parent=MsG0180001549.01.T01
Chr1 CDS 23117834 23117913 23117834 ID=MsG0180001549.01.T01:cds;Parent=MsG0180001549.01.T01
Chr1 CDS 23118001 23118278 23118001 ID=MsG0180001549.01.T01:cds;Parent=MsG0180001549.01.T01
Chr1 CDS 23118812 23119210 23118812 ID=MsG0180001549.01.T01:cds;Parent=MsG0180001549.01.T01
Chr1 CDS 23119298 23119395 23119298 ID=MsG0180001549.01.T01:cds;Parent=MsG0180001549.01.T01
Chr1 CDS 23119618 23119896 23119618 ID=MsG0180001549.01.T01:cds;Parent=MsG0180001549.01.T01
Gene Sequence

>MsG0180001549.01.T01

ATGGTTGAAAAAAGTTCAATAACTCACAAATCGAGTAAATTTGATTTGGATGATCTAAATTGGACAAACATGATTCCAGAATGTCCAGTTTACCATCCATCAGAACAAGAATTTGAGCATCCTTTGGTTTATCTACAGAAAATTGCTCCTGAGGCTTCTAAATATGGTATATGCAAAATTGTTTCTCCAATTAGTGCCTCTAATCCTTCTGATTTTGTCTTAATGAAAGAGAAAAAGGACTTCAAGTTTGAAACAATTGTACAGCCTCTTAGGCTTTCCAAATGGAATGAGAAGGATATAATAACCTTTTCTAAGAGAGGAAGAAAATTTACATATCAAGAATTTGAGGCTATAGCAAACAAGGCCTTTTCCAACAAATTTTGCAGTTCAGAAGATCTTTCTTCGTTAGATATCGAAAAAGCATTCTGGCACGAAATGATACATGGAGAGAAGGGAACAGTTGAGTATGGAGTCAACATTGAGGGTAGTGTCTTTTCATGTGATCCTGATGATGAACTTGGAATGAGCAAATTCAATTTGAAGAATTTGGCGCGGCTGCCGCAGTCTCCATTGCGTTTAGTTGACAGAGGAATTCCTGGAATAACTGATCCTATGCTTTACATTGGGATGCTATTCAGTATGTTTGCATGGCATGTAGAAGATCACTATTTGTACAGTATAAATTATCATCACTCAGGTGGAAATAAAACTTGGTATGGCGTGCCTAGCTCTGCAGCCTCTCAATTTGAAAAGACTGTCTTGAATCATGTCTATTGCAACAAAATCTTAGCAGAACATGGAGAGAATGGTGCATTCCAATTTCTTGCACAAAAAACAACCATGTTCCCTCCTAATGTCTTGTTACAACATGATGTGCCAGTTTACAAGGCGGTGCAAAAGCCAGGAGAGTTTGTCATCACCTTTCCTAATTCATATCATGCTGGATTTAGTCATGGTTTTAACTGTGGAGAAGCTGTAAACTTTGCTATTGGTGATTGGTTTCCATTTGGGGCTGCAGCAAGCAAACGTTATTCACATCTTAAAATGCTACCTATAATTCCATATGAGGAACTTCTTTGTAAAGAAGCAATGCTTATCTACAAGTCCTCAAAAGTCAGAAGTTACAAAAGCAAACTCGAAGCCATGGCATCTTATTGTGCCATTGTACAGTCTTTCTGGCATCTTATGCAATTTTATAAGACATCACTATCTCGATTGAGCAATTCAAGAAAGTCATCAAGATCTTCAAATACATCATTAGGTTCTGTGACATGCAGCCTCTGCCATAGGGATTGTTATGTAGCTTACTTGTTGTGCAAGAAATGCTATTCCTATCCAATTTGCCTTTTCCATGACATTGTGCCAAAAACTTGCTTATGTGGGGGAAAATATACCGTTTTTAAGAAGAATGACATGTCAGAACTCGAAGATGCTGCCAAAAGTTTTCAACAGAATGATAATCGGCATGAAGAAAAAAGTTCTAGAAGAACTGTCAACTCTGTAAGGGCAGTTTCTTCTAAACTGAAAACAAGAGTCACGGACAACTTCAAGCAAAGTGTTGTAAAGAATCAGAAGATGACAAGGAATCATCGTAAAAACAATACTTCTCCATCAGTGATGAGGGCCAAAAGACCGCGAACCCTTTATAATTTGAGAAAACATGAATCAAAATTGATCATAGAAGCTCCTGCAATATCTTCTGTTTTGGTGAGATCTGAAAAATCAAACTAA
Protein sequence

>MsG0180001549.01.T01

MVEKSSITHKSSKFDLDDLNWTNMIPECPVYHPSEQEFEHPLVYLQKIAPEASKYGICKIVSPISASNPSDFVLMKEKKDFKFETIVQPLRLSKWNEKDIITFSKRGRKFTYQEFEAIANKAFSNKFCSSEDLSSLDIEKAFWHEMIHGEKGTVEYGVNIEGSVFSCDPDDELGMSKFNLKNLARLPQSPLRLVDRGIPGITDPMLYIGMLFSMFAWHVEDHYLYSINYHHSGGNKTWYGVPSSAASQFEKTVLNHVYCNKILAEHGENGAFQFLAQKTTMFPPNVLLQHDVPVYKAVQKPGEFVITFPNSYHAGFSHGFNCGEAVNFAIGDWFPFGAAASKRYSHLKMLPIIPYEELLCKEAMLIYKSSKVRSYKSKLEAMASYCAIVQSFWHLMQFYKTSLSRLSNSRKSSRSSNTSLGSVTCSLCHRDCYVAYLLCKKCYSYPICLFHDIVPKTCLCGGKYTVFKKNDMSELEDAAKSFQQNDNRHEEKSSRRTVNSVRAVSSKLKTRVTDNFKQSVVKNQKMTRNHRKNNTSPSVMRAKRPRTLYNLRKHESKLIIEAPAISSVLVRSEKSN*