AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180001839.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180001839.01.T01 MTR_1g045040 83.465 127 21 0 45 171 2 128 5.05e-67 206
MsG0180001839.01.T01 MTR_1g089830 75.781 128 24 2 45 171 2 123 2.78e-62 194
MsG0180001839.01.T01 MTR_7g055787 62.595 131 49 0 178 308 17 147 5.38e-48 158
MsG0180001839.01.T01 MTR_8g468290 67.708 96 31 0 213 308 1 96 3.24e-41 139
MsG0180001839.01.T01 MTR_8g089170 38.095 147 65 3 49 170 7 152 2.21e-19 83.6
MsG0180001839.01.T01 MTR_8g089130 35.135 148 70 3 49 170 7 154 5.99e-18 79.7
MsG0180001839.01.T01 MTR_8g039350 61.111 54 18 1 37 87 2 55 1.85e-15 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 71 sgRNAs with CRISPR-Local

Find 255 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAAAAGCTTCAAAGTCTA+TGG 0.184389 1:+28019166 MsG0180001839.01.T01:CDS
GTTGATCTTCCTCTTTAGTT+TGG 0.232987 1:-28020614 None:intergenic
TGGAAAATATTACCATAAAA+AGG 0.302586 1:-28016709 None:intergenic
TTGATCTTCCTCTTTAGTTT+GGG 0.313171 1:-28020613 None:intergenic
GTGGCACTTGTCTATTGGTT+TGG 0.326069 1:+28016673 MsG0180001839.01.T01:CDS
TGGCACTTGTCTATTGGTTT+GGG 0.343085 1:+28016674 MsG0180001839.01.T01:CDS
TACAAGTGGCACTTGTCTAT+TGG 0.352036 1:+28016668 MsG0180001839.01.T01:CDS
TTCAATAGATTCCCTCTACT+TGG 0.372159 1:+28017514 MsG0180001839.01.T01:intron
ACTTTCTTCAATCCTCCAAT+TGG 0.415555 1:-28020682 None:intergenic
ATAACAACAAGGAAAACAAC+TGG 0.420492 1:+28020652 MsG0180001839.01.T01:CDS
GACTTCCTCAATTGGGACCT+TGG 0.421915 1:-28020923 None:intergenic
ATGTGCATGACTTCCTCAAT+TGG 0.428378 1:-28020931 None:intergenic
CCACAAGCATTACATAGTAT+TGG 0.433061 1:-28018574 None:intergenic
CATCCATGAAGCTGACTTGT+CGG 0.439456 1:-28020720 None:intergenic
TGGAGATTGGTTTGAAAGCT+CGG 0.447768 1:-28018701 None:intergenic
GATCCGACAAGTCAGCTTCA+TGG 0.456202 1:+28020717 MsG0180001839.01.T01:CDS
GAAACTAAACTTTGGCGTAA+AGG 0.456777 1:+28018538 MsG0180001839.01.T01:intron
TCAATAGATTCCCTCTACTT+GGG 0.457632 1:+28017515 MsG0180001839.01.T01:intron
GGGGAAATCATATTCAAAGT+AGG 0.459976 1:+28017535 MsG0180001839.01.T01:CDS
TGCTTGTGGACTACATTTCA+AGG 0.461822 1:+28018588 MsG0180001839.01.T01:CDS
TGTATACCAAGCAATGAGAT+AGG 0.462534 1:+28019255 MsG0180001839.01.T01:CDS
CGTGTTTAGCGTTCCAACCC+TGG 0.472161 1:-28021036 None:intergenic
GGACTACATTTCAAGGCTAA+AGG 0.474721 1:+28018595 MsG0180001839.01.T01:CDS
ACAAGAATGCTTTCAGAAGA+AGG 0.477716 1:+28020764 MsG0180001839.01.T01:CDS
TCTTCGGAAGATAGTTATCA+AGG 0.478433 1:-28018621 None:intergenic
GAACAATCGTGTTCTGATCA+AGG 0.480601 1:+28020882 MsG0180001839.01.T01:CDS
AGGGATGATCACATCCAAAC+TGG 0.480707 1:+28017555 MsG0180001839.01.T01:CDS
ATCAGACCTTGACATTGAAA+AGG 0.486095 1:+28018663 MsG0180001839.01.T01:CDS
ATCGATTGTGAAGAATCAAA+TGG 0.491491 1:+28019134 MsG0180001839.01.T01:CDS
CATAGTATTGGCTTCTCATA+CGG 0.492997 1:-28018562 None:intergenic
GGAGATTGGTTTGAAAGCTC+GGG 0.493147 1:-28018700 None:intergenic
TGCAAAATCGAAGTAGTACA+AGG 0.496080 1:+28020965 MsG0180001839.01.T01:CDS
CCAAGCAATGAGATAGGCAT+TGG 0.496933 1:+28019261 MsG0180001839.01.T01:CDS
CAATAGATTCCCTCTACTTG+GGG 0.515255 1:+28017516 MsG0180001839.01.T01:intron
ATAGGCTAACACTCGGCAAA+TGG 0.515872 1:+28016636 MsG0180001839.01.T01:CDS
GGGAAATCATATTCAAAGTA+GGG 0.523564 1:+28017536 MsG0180001839.01.T01:CDS
ATTTGATTCTTCACAATCGA+TGG 0.523804 1:-28019132 None:intergenic
CTACTCTCATGCATAAGTCT+TGG 0.525421 1:+28020827 MsG0180001839.01.T01:CDS
TGTGCATGACTTCCTCAATT+GGG 0.532957 1:-28020930 None:intergenic
ACTTGTCGGATCTCCTCTTG+TGG 0.542057 1:-28020706 None:intergenic
CAGATGTGAGTTATTTCGAA+AGG 0.543392 1:-28020856 None:intergenic
TGATCAAGGATCAAACTAGA+TGG 0.543491 1:+28020896 MsG0180001839.01.T01:CDS
ACAAGTCAGCTTCATGGATG+AGG 0.543906 1:+28020723 MsG0180001839.01.T01:CDS
AACACTTGGACATATCCGCC+AGG 0.544510 1:+28021018 MsG0180001839.01.T01:CDS
TCAAGGTACCCAAACTAAAG+AGG 0.544907 1:+28020605 MsG0180001839.01.T01:intron
GTTGGCCAAGGTCCCAATTG+AGG 0.555630 1:+28020918 MsG0180001839.01.T01:CDS
ACAAAAGCTTCAAAGTCTAT+GGG 0.556384 1:+28019167 MsG0180001839.01.T01:CDS
CCAATGCCTATCTCATTGCT+TGG 0.566560 1:-28019261 None:intergenic
GAGCTTTCAAACCAATCTCC+AGG 0.568278 1:+28018703 MsG0180001839.01.T01:CDS
GTCTATTGGTTTGGGCAATG+TGG 0.570255 1:+28016682 MsG0180001839.01.T01:CDS
TTGGACATATCCGCCAGGGT+TGG 0.572442 1:+28021023 MsG0180001839.01.T01:CDS
CAAGGATCAAACTAGATGGT+TGG 0.577904 1:+28020900 MsG0180001839.01.T01:CDS
CCAATACTATGTAATGCTTG+TGG 0.579274 1:+28018574 MsG0180001839.01.T01:CDS
GACTTATGCATGAGAGTAGG+TGG 0.580845 1:-28020823 None:intergenic
ATATGATTTCCCCAAGTAGA+GGG 0.592118 1:-28017525 None:intergenic
AATATGATTTCCCCAAGTAG+AGG 0.596666 1:-28017526 None:intergenic
ATTGAAGAAAGTTCCACAAG+AGG 0.598488 1:+28020693 MsG0180001839.01.T01:CDS
CAAGACTTATGCATGAGAGT+AGG 0.615815 1:-28020826 None:intergenic
TAACAACAAGGAAAACAACT+GGG 0.620441 1:+28020653 MsG0180001839.01.T01:CDS
TCAAACTAGATGGTTGGCCA+AGG 0.628204 1:+28020906 MsG0180001839.01.T01:CDS
CTTCGGAAGATAGTTATCAA+GGG 0.629882 1:-28018620 None:intergenic
TGCGGATAGTCCTTATGCAT+AGG 0.630988 1:+28016618 None:intergenic
CTTATGCATAGGCTAACACT+CGG 0.650790 1:+28016629 None:intergenic
AATGGTCATACGATTACAAG+TGG 0.653175 1:+28016654 MsG0180001839.01.T01:CDS
ACACTTGGACATATCCGCCA+GGG 0.670064 1:+28021019 MsG0180001839.01.T01:CDS
GCAAAATCGAAGTAGTACAA+GGG 0.670625 1:+28020966 MsG0180001839.01.T01:CDS
GTTTAGCGTTCCAACCCTGG+CGG 0.679983 1:-28021033 None:intergenic
GATTGTGAAGAATCAAATGG+AGG 0.680011 1:+28019137 MsG0180001839.01.T01:CDS
AACAACAAGGAAAACAACTG+GGG 0.698459 1:+28020654 MsG0180001839.01.T01:CDS
ACTAAAGAGGAAGATCAACA+TGG 0.714540 1:+28020618 MsG0180001839.01.T01:CDS
TGAGAACAAAGATAACAACA+AGG 0.765280 1:+28020641 MsG0180001839.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAAAAAAATAAATGAG+TGG - Chr1:28020189-28020208 None:intergenic 10.0%
!! ATAAAAAAAAATAAATGAGT+GGG - Chr1:28020188-28020207 None:intergenic 10.0%
!! ATTAAATATATACCAAAATA+TGG - Chr1:28019557-28019576 None:intergenic 10.0%
!! TAAAAGTTAATGAAATATTA+TGG - Chr1:28019637-28019656 None:intergenic 10.0%
!! TATACTAAAATTTAACAATT+AGG - Chr1:28017878-28017897 None:intergenic 10.0%
!!! ACTGTTTTTTTTTTATATAA+AGG + Chr1:28018324-28018343 MsG0180001839.01.T01:intron 10.0%
!!! ATCAAATTTAGATTTTAAAT+AGG + Chr1:28016817-28016836 MsG0180001839.01.T01:intron 10.0%
!!! TCAAATTTAGATTTTAAATA+GGG + Chr1:28016818-28016837 MsG0180001839.01.T01:intron 10.0%
!!! TTTTAATTTTTTTTGTAGAA+AGG + Chr1:28019106-28019125 MsG0180001839.01.T01:intron 10.0%
!!! TTTTTCATAAAATTGTAATT+TGG + Chr1:28017965-28017984 MsG0180001839.01.T01:intron 10.0%
!! AAAAAAAAAAATACTCAACT+AGG - Chr1:28020032-28020051 None:intergenic 15.0%
!! AAAAACAAAAAGAAAAGAAA+TGG - Chr1:28020149-28020168 None:intergenic 15.0%
!! AACTAAGATTAATTATCATT+AGG + Chr1:28020494-28020513 MsG0180001839.01.T01:intron 15.0%
!! AATATTATTATTTGTGTATG+TGG - Chr1:28017719-28017738 None:intergenic 15.0%
!! AATTTGATTGTATCTATTTA+TGG - Chr1:28018423-28018442 None:intergenic 15.0%
!! ATTCTAAATGCAAAATTAAA+TGG - Chr1:28020353-28020372 None:intergenic 15.0%
!! GGTTTATTAGTAATAATATT+AGG + Chr1:28020411-28020430 MsG0180001839.01.T01:intron 15.0%
!! TAAAAAAAAATAAATGAGTG+GGG - Chr1:28020187-28020206 None:intergenic 15.0%
!! TTCGTTATTATTAGATAAAA+TGG + Chr1:28018368-28018387 MsG0180001839.01.T01:intron 15.0%
!!! CAAATTTAGATTTTAAATAG+GGG + Chr1:28016819-28016838 MsG0180001839.01.T01:intron 15.0%
!!! CTTTATATTTGTTGAAAAAT+GGG - Chr1:28018764-28018783 None:intergenic 15.0%
!!! TGTTTATTTTTAAGTTAACA+TGG + Chr1:28018507-28018526 MsG0180001839.01.T01:intron 15.0%
!!! TTAATTTTGCATTTAGAATT+AGG + Chr1:28020354-28020373 MsG0180001839.01.T01:intron 15.0%
!!! TTGGATTTTATTTTTTAGAT+CGG - Chr1:28017600-28017619 None:intergenic 15.0%
!!! TTTATATTTGTTGAAAAATG+GGG - Chr1:28018763-28018782 None:intergenic 15.0%
!! AAATATTACCATAAAAAGGT+TGG - Chr1:28016708-28016727 None:intergenic 20.0%
!! AACACTACTATTTATATATG+AGG + Chr1:28018869-28018888 MsG0180001839.01.T01:intron 20.0%
!! AATACCAATCTTATTAGTTA+AGG - Chr1:28018288-28018307 None:intergenic 20.0%
!! ACTATAGTAGTTAATTTAGA+TGG + Chr1:28020439-28020458 MsG0180001839.01.T01:intron 20.0%
!! ATAGATTTATTGGATCTATT+CGG + Chr1:28017626-28017645 MsG0180001839.01.T01:intron 20.0%
!! CCAAAAAAACATAGATTTAT+TGG + Chr1:28017616-28017635 MsG0180001839.01.T01:intron 20.0%
!! CTAAAATTTAACAATTAGGA+AGG - Chr1:28017874-28017893 None:intergenic 20.0%
!! TGGAAAATATTACCATAAAA+AGG - Chr1:28016712-28016731 None:intergenic 20.0%
!! TTATATGTATACATCATACA+CGG - Chr1:28019084-28019103 None:intergenic 20.0%
!! TTTCAAATGTCAACATAATT+GGG + Chr1:28018218-28018237 MsG0180001839.01.T01:intron 20.0%
!!! AACACGAGTATTTAAATTTT+TGG + Chr1:28018072-28018091 MsG0180001839.01.T01:intron 20.0%
!!! ATTGTAAGAATTTTCTTAAG+AGG + Chr1:28018818-28018837 MsG0180001839.01.T01:intron 20.0%
!!! ATTTTATTTTTTAGATCGGT+TGG - Chr1:28017596-28017615 None:intergenic 20.0%
!!! ATTTTGAGAAATGATTTTGA+AGG + Chr1:28019509-28019528 MsG0180001839.01.T01:intron 20.0%
!!! CCAATAAATCTATGTTTTTT+TGG - Chr1:28017619-28017638 None:intergenic 20.0%
!!! GCTTTATATTTGTTGAAAAA+TGG - Chr1:28018765-28018784 None:intergenic 20.0%
!!! TATCTACGTTTTCTATTTAT+TGG + Chr1:28018171-28018190 MsG0180001839.01.T01:intron 20.0%
!!! TTATTGCATTTTTTATTACC+TGG - Chr1:28018724-28018743 None:intergenic 20.0%
!!! TTCTTTTTGTTTTTCCATTT+AGG + Chr1:28020155-28020174 MsG0180001839.01.T01:intron 20.0%
!!! TTTGAATGAATTGTATTGAT+GGG + Chr1:28018944-28018963 MsG0180001839.01.T01:intron 20.0%
!!! TTTTCAAATGTCAACATAAT+TGG + Chr1:28018217-28018236 MsG0180001839.01.T01:intron 20.0%
!!! TTTTCCTTAACTAATAAGAT+TGG + Chr1:28018281-28018300 MsG0180001839.01.T01:intron 20.0%
!!! TTTTGAATGAATTGTATTGA+TGG + Chr1:28018943-28018962 MsG0180001839.01.T01:intron 20.0%
! AAATTACAATATTCCATGAC+GGG + Chr1:28017435-28017454 MsG0180001839.01.T01:intron 25.0%
! AAGAAATGGTTAGTACTAAA+TGG - Chr1:28020135-28020154 None:intergenic 25.0%
! ACATGGTCAAAGAATAAATA+CGG + Chr1:28017298-28017317 MsG0180001839.01.T01:intron 25.0%
! ACATTTGAAAATAACCACTA+TGG - Chr1:28018208-28018227 None:intergenic 25.0%
! ACTTCGAAATATGATATTGA+GGG - Chr1:28019705-28019724 None:intergenic 25.0%
! AGATAAAACAAAATACAAGG+TGG - Chr1:28018156-28018175 None:intergenic 25.0%
! AGCTATTAAGATTAACACAA+AGG + Chr1:28017131-28017150 MsG0180001839.01.T01:intron 25.0%
! CAAATTACAATATTCCATGA+CGG + Chr1:28017434-28017453 MsG0180001839.01.T01:intron 25.0%
! CAACAAAAAAAATAGACGTA+TGG + Chr1:28017158-28017177 MsG0180001839.01.T01:intron 25.0%
! CGTAGATAAAACAAAATACA+AGG - Chr1:28018159-28018178 None:intergenic 25.0%
! TAAAGACAGAGTAAAATCAT+AGG - Chr1:28017925-28017944 None:intergenic 25.0%
! TACTCAAATGTATGAATGTA+GGG - Chr1:28018909-28018928 None:intergenic 25.0%
! TATCCATAAATGTATGAATG+TGG - Chr1:28018993-28019012 None:intergenic 25.0%
! TATTATTTGTGTATGTGGTT+TGG - Chr1:28017714-28017733 None:intergenic 25.0%
! TGATTGTATCTATTTATGGT+TGG - Chr1:28018419-28018438 None:intergenic 25.0%
! TTATCTCTTAAACTTTCGAT+TGG + Chr1:28019454-28019473 MsG0180001839.01.T01:intron 25.0%
!! ATGTTTAGATACAGTTTTCA+AGG - Chr1:28017068-28017087 None:intergenic 25.0%
!! CAACTAGGACTAAATATTTT+TGG - Chr1:28020017-28020036 None:intergenic 25.0%
!! CATTGTCATTTATATTCACA+TGG - Chr1:28016762-28016781 None:intergenic 25.0%
!! TATTTTCAGAGTCTTAGTTT+AGG + Chr1:28020211-28020230 MsG0180001839.01.T01:intron 25.0%
!! TTGAATGAATTGTATTGATG+GGG + Chr1:28018945-28018964 MsG0180001839.01.T01:intron 25.0%
!! TTTCTTAAGAGGTACAATTT+AGG + Chr1:28018829-28018848 MsG0180001839.01.T01:intron 25.0%
!! TTTTGACAGAAACTAAACTT+TGG + Chr1:28018530-28018549 MsG0180001839.01.T01:intron 25.0%
!!! ATTTTTTATTACCTGGAGAT+TGG - Chr1:28018717-28018736 None:intergenic 25.0%
!!! GTTTGGTTTGATTTTGAAAA+AGG - Chr1:28017697-28017716 None:intergenic 25.0%
!!! TGTTTTAATTTAGACCAGTT+TGG - Chr1:28017572-28017591 None:intergenic 25.0%
!!! TTCTTGTCTTTGATTTTGAT+TGG - Chr1:28020751-28020770 None:intergenic 25.0%
AAACCTAAAGCTTGGTTTAT+CGG + Chr1:28017336-28017355 MsG0180001839.01.T01:intron 30.0%
AACAAAAGCTTCAAAGTCTA+TGG + Chr1:28019166-28019185 MsG0180001839.01.T01:CDS 30.0%
AACTGATCTAAATCATACCA+TGG + Chr1:28018117-28018136 MsG0180001839.01.T01:intron 30.0%
AAGTAATCTTGTTCAAGACA+TGG + Chr1:28017281-28017300 MsG0180001839.01.T01:intron 30.0%
AATTCAGATTGGATGAATTC+TGG - Chr1:28018050-28018069 None:intergenic 30.0%
ACAAAAGCTTCAAAGTCTAT+GGG + Chr1:28019167-28019186 MsG0180001839.01.T01:CDS 30.0%
ACATGTCTAAAAACAACACT+TGG + Chr1:28021004-28021023 MsG0180001839.01.T01:CDS 30.0%
ACTATTAGTCATAGAGTCTT+AGG + Chr1:28020390-28020409 MsG0180001839.01.T01:intron 30.0%
ACTCCACATTCATACATTTA+TGG + Chr1:28018987-28019006 MsG0180001839.01.T01:intron 30.0%
ACTGATCTAAATCATACCAT+GGG + Chr1:28018118-28018137 MsG0180001839.01.T01:intron 30.0%
ATAACAACAAGGAAAACAAC+TGG + Chr1:28020652-28020671 MsG0180001839.01.T01:CDS 30.0%
ATCGATTGTGAAGAATCAAA+TGG + Chr1:28019134-28019153 MsG0180001839.01.T01:CDS 30.0%
ATGAGAGTCACATTAAAAAC+TGG - Chr1:28018468-28018487 None:intergenic 30.0%
ATTTCGAAGTCTATTCTTGA+TGG + Chr1:28019715-28019734 MsG0180001839.01.T01:intron 30.0%
ATTTGATTCTTCACAATCGA+TGG - Chr1:28019135-28019154 None:intergenic 30.0%
CTACTCAAATGTATGAATGT+AGG - Chr1:28018910-28018929 None:intergenic 30.0%
CTTCGAAATATGATATTGAG+GGG - Chr1:28019704-28019723 None:intergenic 30.0%
GAAGAACAATTTCGTGTATT+CGG + Chr1:28019779-28019798 MsG0180001839.01.T01:intron 30.0%
GACTTCGAAATATGATATTG+AGG - Chr1:28019706-28019725 None:intergenic 30.0%
GATCGAGTCAATTTAACATT+AGG + Chr1:28020304-28020323 MsG0180001839.01.T01:intron 30.0%
GGGAAATCATATTCAAAGTA+GGG + Chr1:28017536-28017555 MsG0180001839.01.T01:CDS 30.0%
GTGTTGTATTAACTTCTTCA+AGG + Chr1:28020588-28020607 MsG0180001839.01.T01:intron 30.0%
TAACAACAAGGAAAACAACT+GGG + Chr1:28020653-28020672 MsG0180001839.01.T01:CDS 30.0%
TAGCCTAGTATATTGTGAAT+CGG + Chr1:28019344-28019363 MsG0180001839.01.T01:intron 30.0%
TGAGAACAAAGATAACAACA+AGG + Chr1:28020641-28020660 MsG0180001839.01.T01:CDS 30.0%
TTCAATTGAAACCTAAAGCT+TGG + Chr1:28017328-28017347 MsG0180001839.01.T01:intron 30.0%
TTCATACATTTGAGTAGACA+CGG + Chr1:28018913-28018932 MsG0180001839.01.T01:intron 30.0%
! AAATAGACGTATGGTACTTT+TGG + Chr1:28017167-28017186 MsG0180001839.01.T01:intron 30.0%
! AGGGTATGTTTATTCTTTTG+TGG - Chr1:28019875-28019894 None:intergenic 30.0%
! ATGGTTAGGCCATAATATTT+TGG + Chr1:28019748-28019767 MsG0180001839.01.T01:intron 30.0%
! CATCTAGTTTACAATTAGAG+AGG - Chr1:28020558-28020577 None:intergenic 30.0%
! CTTTAGGTTTCAATTGAAAG+AGG - Chr1:28017326-28017345 None:intergenic 30.0%
! GTATCTATTTATGGTTGGTT+AGG - Chr1:28018414-28018433 None:intergenic 30.0%
! TACAATTAGAGAGGTTGATT+AGG - Chr1:28020549-28020568 None:intergenic 30.0%
! TGGTTAGGCCATAATATTTT+GGG + Chr1:28019749-28019768 MsG0180001839.01.T01:intron 30.0%
! TTTTAAATAGGGGAAGTCAT+AGG + Chr1:28016829-28016848 MsG0180001839.01.T01:intron 30.0%
! TTTTTAGACATGTGTGTATC+TGG - Chr1:28020997-28021016 None:intergenic 30.0%
!! AGGAAGCAGCTTTAAATATT+TGG - Chr1:28017048-28017067 None:intergenic 30.0%
!! CCATAATATTTTGGGTGTTA+CGG + Chr1:28019757-28019776 MsG0180001839.01.T01:intron 30.0%
!!! TCGGTGTTAAATCCATATTT+TGG + Chr1:28019542-28019561 MsG0180001839.01.T01:intron 30.0%
!!! TCTTCACTTGCTCTTTTTTT+GGG - Chr1:28017008-28017027 None:intergenic 30.0%
AACAACAAGGAAAACAACTG+GGG + Chr1:28020654-28020673 MsG0180001839.01.T01:CDS 35.0%
AATATGATTTCCCCAAGTAG+AGG - Chr1:28017529-28017548 None:intergenic 35.0%
AATGGTCATACGATTACAAG+TGG + Chr1:28016654-28016673 MsG0180001839.01.T01:CDS 35.0%
ACAAGAATGCTTTCAGAAGA+AGG + Chr1:28020764-28020783 MsG0180001839.01.T01:CDS 35.0%
ACAATTAAACGTGGAGAAGT+GGG - Chr1:28016958-28016977 None:intergenic 35.0%
ACTCGTGTTTCAATTCAGAT+TGG - Chr1:28018061-28018080 None:intergenic 35.0%
ACTTTCTTCAATCCTCCAAT+TGG - Chr1:28020685-28020704 None:intergenic 35.0%
ATATGATTTCCCCAAGTAGA+GGG - Chr1:28017528-28017547 None:intergenic 35.0%
ATCAGACCTTGACATTGAAA+AGG + Chr1:28018663-28018682 MsG0180001839.01.T01:CDS 35.0%
ATTGAAGAAAGTTCCACAAG+AGG + Chr1:28020693-28020712 MsG0180001839.01.T01:CDS 35.0%
ATTTGAGTAGACACGGTATT+AGG + Chr1:28018920-28018939 MsG0180001839.01.T01:intron 35.0%
CAAACTAAACCGCCTATAAT+TGG + Chr1:28018005-28018024 MsG0180001839.01.T01:intron 35.0%
CCAATACTATGTAATGCTTG+TGG + Chr1:28018574-28018593 MsG0180001839.01.T01:CDS 35.0%
CCACAAGCATTACATAGTAT+TGG - Chr1:28018577-28018596 None:intergenic 35.0%
CCGTAACACCCAAAATATTA+TGG - Chr1:28019760-28019779 None:intergenic 35.0%
CTTCGGAAGATAGTTATCAA+GGG - Chr1:28018623-28018642 None:intergenic 35.0%
GAAACTAAACTTTGGCGTAA+AGG + Chr1:28018538-28018557 MsG0180001839.01.T01:intron 35.0%
GATTGTGAAGAATCAAATGG+AGG + Chr1:28019137-28019156 MsG0180001839.01.T01:CDS 35.0%
GCAAAATCGAAGTAGTACAA+GGG + Chr1:28020966-28020985 MsG0180001839.01.T01:CDS 35.0%
GCGAGTCGTAGTAAAAATAA+CGG + Chr1:28020261-28020280 MsG0180001839.01.T01:intron 35.0%
GGACATTGCATAATTTATGC+AGG + Chr1:28016908-28016927 MsG0180001839.01.T01:intron 35.0%
GGGGAAATCATATTCAAAGT+AGG + Chr1:28017535-28017554 MsG0180001839.01.T01:CDS 35.0%
GTCTGATCTGTTCCAATTAT+AGG - Chr1:28018020-28018039 None:intergenic 35.0%
TCAATAGATTCCCTCTACTT+GGG + Chr1:28017515-28017534 MsG0180001839.01.T01:intron 35.0%
TCATACACGGTACATATCAA+TGG - Chr1:28019071-28019090 None:intergenic 35.0%
TCTTCGGAAGATAGTTATCA+AGG - Chr1:28018624-28018643 None:intergenic 35.0%
TGATCAAGGATCAAACTAGA+TGG + Chr1:28020896-28020915 MsG0180001839.01.T01:CDS 35.0%
TGATCTGTTCCAATTATAGG+CGG - Chr1:28018017-28018036 None:intergenic 35.0%
TGCAAAATCGAAGTAGTACA+AGG + Chr1:28020965-28020984 MsG0180001839.01.T01:CDS 35.0%
TGTATACCAAGCAATGAGAT+AGG + Chr1:28019255-28019274 MsG0180001839.01.T01:CDS 35.0%
TGTGTGCATTGTGGAATTAA+TGG + Chr1:28017462-28017481 MsG0180001839.01.T01:intron 35.0%
TTAATTCCACAATGCACACA+TGG - Chr1:28017462-28017481 None:intergenic 35.0%
TTATCACATAGCTCTCTGTT+AGG + Chr1:28017234-28017253 MsG0180001839.01.T01:intron 35.0%
TTCAATAGATTCCCTCTACT+TGG + Chr1:28017514-28017533 MsG0180001839.01.T01:intron 35.0%
TTGAGTTAAGATGAATGAGC+AGG - Chr1:28017417-28017436 None:intergenic 35.0%
TTTGTAAAAACCGCTCTGAT+TGG + Chr1:28017656-28017675 MsG0180001839.01.T01:intron 35.0%
! AATGAAGCATCTCAAGAGAT+AGG - Chr1:28019947-28019966 None:intergenic 35.0%
! ATGGTCTATTCTTGATGGTT+AGG + Chr1:28019734-28019753 MsG0180001839.01.T01:intron 35.0%
! CAGATGTGAGTTATTTCGAA+AGG - Chr1:28020859-28020878 None:intergenic 35.0%
! CAGATTCCTTTTCAATGTCA+AGG - Chr1:28018672-28018691 None:intergenic 35.0%
! CTCTCTGTTAGGTTTTCTAT+AGG + Chr1:28017245-28017264 MsG0180001839.01.T01:intron 35.0%
! GAATTGTATTGATGGGGATT+AGG + Chr1:28018951-28018970 MsG0180001839.01.T01:intron 35.0%
! GATGCGTTGCATTTTGAATA+GGG - Chr1:28019836-28019855 None:intergenic 35.0%
! TCTTGATGGTCTATTCTTGA+TGG + Chr1:28019729-28019748 MsG0180001839.01.T01:intron 35.0%
! TGATGCGTTGCATTTTGAAT+AGG - Chr1:28019837-28019856 None:intergenic 35.0%
!! AATCATACCATGGGTTTTGA+TGG + Chr1:28018127-28018146 MsG0180001839.01.T01:intron 35.0%
!! AATGATTTTGAAGGAGAGAG+TGG + Chr1:28019518-28019537 MsG0180001839.01.T01:intron 35.0%
!! ATAGGCGGTTTAGTTTGTAT+CGG - Chr1:28018002-28018021 None:intergenic 35.0%
!! ATGCCGATTCACAATATACT+AGG - Chr1:28019350-28019369 None:intergenic 35.0%
!! CATAGTATTGGCTTCTCATA+CGG - Chr1:28018565-28018584 None:intergenic 35.0%
!! GTCTGATAACTGATTTTGCT+GGG - Chr1:28018650-28018669 None:intergenic 35.0%
!!! GTCTTCACTTGCTCTTTTTT+TGG - Chr1:28017009-28017028 None:intergenic 35.0%
AAATAGGGGAAGTCATAGGA+AGG + Chr1:28016833-28016852 MsG0180001839.01.T01:intron 40.0%
AAGCCGATAAACCAAGCTTT+AGG - Chr1:28017342-28017361 None:intergenic 40.0%
AATTTCGTGTATTCGGAGAC+TGG + Chr1:28019786-28019805 MsG0180001839.01.T01:intron 40.0%
ACAACTGGGGAAAAACCAAT+TGG + Chr1:28020667-28020686 MsG0180001839.01.T01:CDS 40.0%
AGAGCTCACACAATTAAACG+TGG - Chr1:28016967-28016986 None:intergenic 40.0%
ATGTGCATGACTTCCTCAAT+TGG - Chr1:28020934-28020953 None:intergenic 40.0%
CAAGACTTATGCATGAGAGT+AGG - Chr1:28020829-28020848 None:intergenic 40.0%
CAAGGATCAAACTAGATGGT+TGG + Chr1:28020900-28020919 MsG0180001839.01.T01:CDS 40.0%
CAATAGATTCCCTCTACTTG+GGG + Chr1:28017516-28017535 MsG0180001839.01.T01:intron 40.0%
CAATTAAACGTGGAGAAGTG+GGG - Chr1:28016957-28016976 None:intergenic 40.0%
CACAATTAAACGTGGAGAAG+TGG - Chr1:28016959-28016978 None:intergenic 40.0%
CTACTCTCATGCATAAGTCT+TGG + Chr1:28020827-28020846 MsG0180001839.01.T01:CDS 40.0%
CTACTGAATTCAAGCTCAAC+TGG + Chr1:28017380-28017399 MsG0180001839.01.T01:intron 40.0%
GAACAATCGTGTTCTGATCA+AGG + Chr1:28020882-28020901 MsG0180001839.01.T01:CDS 40.0%
GGACTACATTTCAAGGCTAA+AGG + Chr1:28018595-28018614 MsG0180001839.01.T01:CDS 40.0%
TAAACATACCCTAGAGACTG+AGG + Chr1:28019883-28019902 MsG0180001839.01.T01:intron 40.0%
TACAAGTGGCACTTGTCTAT+TGG + Chr1:28016668-28016687 MsG0180001839.01.T01:CDS 40.0%
TCAAGAGATAGGCAAGCAAT+AGG - Chr1:28019936-28019955 None:intergenic 40.0%
TGTGCATGACTTCCTCAATT+GGG - Chr1:28020933-28020952 None:intergenic 40.0%
! AGATGGACTCTGTTAAGTCT+AGG + Chr1:28020456-28020475 MsG0180001839.01.T01:intron 40.0%
! CAATGTGGCCAACCTTTTTA+TGG + Chr1:28016697-28016716 MsG0180001839.01.T01:CDS 40.0%
! TCAAGGTACCCAAACTAAAG+AGG + Chr1:28020605-28020624 MsG0180001839.01.T01:intron 40.0%
! TGATTTTGCTGGGATGTCTT+CGG - Chr1:28018640-28018659 None:intergenic 40.0%
! TGCTTGTGGACTACATTTCA+AGG + Chr1:28018588-28018607 MsG0180001839.01.T01:CDS 40.0%
!! GGTCTGATAACTGATTTTGC+TGG - Chr1:28018651-28018670 None:intergenic 40.0%
!! TGGAGATTGGTTTGAAAGCT+CGG - Chr1:28018704-28018723 None:intergenic 40.0%
!! TGGCACTTGTCTATTGGTTT+GGG + Chr1:28016674-28016693 MsG0180001839.01.T01:CDS 40.0%
!! TTTTGAAGGAGAGAGTGGTT+CGG + Chr1:28019523-28019542 MsG0180001839.01.T01:intron 40.0%
!!! ATTTGTTTTTTCCGCGTGTC+GGG + Chr1:28017086-28017105 MsG0180001839.01.T01:intron 40.0%
!!! CATTTGTTTTTTCCGCGTGT+CGG + Chr1:28017085-28017104 MsG0180001839.01.T01:intron 40.0%
ACAAGTCAGCTTCATGGATG+AGG + Chr1:28020723-28020742 MsG0180001839.01.T01:CDS 45.0%
ACTGGGGAAAAACCAATTGG+AGG + Chr1:28020670-28020689 MsG0180001839.01.T01:CDS 45.0%
AGGGATGATCACATCCAAAC+TGG + Chr1:28017555-28017574 MsG0180001839.01.T01:CDS 45.0%
ATAATACACAACCCGACACG+CGG - Chr1:28017100-28017119 None:intergenic 45.0%
ATAGGCTAACACTCGGCAAA+TGG + Chr1:28016636-28016655 MsG0180001839.01.T01:CDS 45.0%
ATCCTCAACCTCAGTCTCTA+GGG - Chr1:28019894-28019913 None:intergenic 45.0%
CATAATTTATGCAGGAGCCG+AGG + Chr1:28016916-28016935 MsG0180001839.01.T01:intron 45.0%
CATACACGATCCAATCAGAG+CGG - Chr1:28017669-28017688 None:intergenic 45.0%
CCAAGCAATGAGATAGGCAT+TGG + Chr1:28019261-28019280 MsG0180001839.01.T01:CDS 45.0%
CCAATGCCTATCTCATTGCT+TGG - Chr1:28019264-28019283 None:intergenic 45.0%
GACTTATGCATGAGAGTAGG+TGG - Chr1:28020826-28020845 None:intergenic 45.0%
GAGCTTTCAAACCAATCTCC+AGG + Chr1:28018703-28018722 MsG0180001839.01.T01:CDS 45.0%
TACCAACTGAGCTAAGCTCA+CGG - Chr1:28016887-28016906 None:intergenic 45.0%
TATCCTCAACCTCAGTCTCT+AGG - Chr1:28019895-28019914 None:intergenic 45.0%
! ATGGTTGGTTAGGCATGTTG+AGG - Chr1:28018404-28018423 None:intergenic 45.0%
! GCGTTGCATTTTGAATAGGG+AGG - Chr1:28019833-28019852 None:intergenic 45.0%
! GTGGCACTTGTCTATTGGTT+TGG + Chr1:28016673-28016692 MsG0180001839.01.T01:CDS 45.0%
! TCAAACTAGATGGTTGGCCA+AGG + Chr1:28020906-28020925 MsG0180001839.01.T01:CDS 45.0%
! TGAGCTTAGCTCAGTTGGTA+GGG + Chr1:28016887-28016906 MsG0180001839.01.T01:intron 45.0%
!! CATCCATGAAGCTGACTTGT+CGG - Chr1:28020723-28020742 None:intergenic 45.0%
!! GAGTGGGGTTAGTTCCTAAA+TGG - Chr1:28020172-28020191 None:intergenic 45.0%
!! GGAGATTGGTTTGAAAGCTC+GGG - Chr1:28018703-28018722 None:intergenic 45.0%
!! GTCTATTGGTTTGGGCAATG+TGG + Chr1:28016682-28016701 MsG0180001839.01.T01:CDS 45.0%
!! TTTTGGGTCTAGTAGCGTAG+TGG - Chr1:28016992-28017011 None:intergenic 45.0%
!!! ATAATTTATATTTTTCAAAA+TGG - Chr1:28016732-28016751 None:intergenic 5.0%
!!! TTTTTTATTTTTTTAAATTG+AGG - Chr1:28019677-28019696 None:intergenic 5.0%
AACACTTGGACATATCCGCC+AGG + Chr1:28021018-28021037 MsG0180001839.01.T01:CDS 50.0%
ACACTTGGACATATCCGCCA+GGG + Chr1:28021019-28021038 MsG0180001839.01.T01:CDS 50.0%
ACCAACTGAGCTAAGCTCAC+GGG - Chr1:28016886-28016905 None:intergenic 50.0%
ACTTGTCGGATCTCCTCTTG+TGG - Chr1:28020709-28020728 None:intergenic 50.0%
AGGGGAAGTCATAGGAAGGT+GGG + Chr1:28016837-28016856 MsG0180001839.01.T01:intron 50.0%
CGCTCTGATTGGATCGTGTA+TGG + Chr1:28017667-28017686 MsG0180001839.01.T01:intron 50.0%
GACTTCCTCAATTGGGACCT+TGG - Chr1:28020926-28020945 None:intergenic 50.0%
GATCCGACAAGTCAGCTTCA+TGG + Chr1:28020717-28020736 MsG0180001839.01.T01:CDS 50.0%
GCAAGCAATAGGAGCCTCAA+GGG - Chr1:28019925-28019944 None:intergenic 50.0%
GTGAGCTTAGCTCAGTTGGT+AGG + Chr1:28016886-28016905 MsG0180001839.01.T01:intron 50.0%
GTGGACACCATCAAAACCCA+TGG - Chr1:28018137-28018156 None:intergenic 50.0%
TACCCTAGAGACTGAGGTTG+AGG + Chr1:28019889-28019908 MsG0180001839.01.T01:intron 50.0%
TAGGGGAAGTCATAGGAAGG+TGG + Chr1:28016836-28016855 MsG0180001839.01.T01:intron 50.0%
TGGAAACTAGCGAGCCATAG+TGG + Chr1:28018191-28018210 MsG0180001839.01.T01:intron 50.0%
!! TAGGGAGGTTGATGAAGTCG+AGG - Chr1:28019818-28019837 None:intergenic 50.0%
ACGGGTCCATGTGTGCATTG+TGG + Chr1:28017453-28017472 MsG0180001839.01.T01:intron 55.0%
AGCAATAGGAGCCTCAAGGG+AGG - Chr1:28019922-28019941 None:intergenic 55.0%
ATGCACACATGGACCCGTCA+TGG - Chr1:28017451-28017470 None:intergenic 55.0%
CCAACTGAGCTAAGCTCACG+GGG - Chr1:28016885-28016904 None:intergenic 55.0%
GAGGATAGACTCCTCCCTTG+AGG + Chr1:28019908-28019927 MsG0180001839.01.T01:intron 55.0%
GGCAAGCAATAGGAGCCTCA+AGG - Chr1:28019926-28019945 None:intergenic 55.0%
GGGGAAGTCATAGGAAGGTG+GGG + Chr1:28016838-28016857 MsG0180001839.01.T01:intron 55.0%
GTTGGCCAAGGTCCCAATTG+AGG + Chr1:28020918-28020937 MsG0180001839.01.T01:CDS 55.0%
TTGGACATATCCGCCAGGGT+TGG + Chr1:28021023-28021042 MsG0180001839.01.T01:CDS 55.0%
!! GGAGGTTGATGAAGTCGAGG+AGG - Chr1:28019815-28019834 None:intergenic 55.0%
CCCCGTGAGCTTAGCTCAGT+TGG + Chr1:28016882-28016901 MsG0180001839.01.T01:intron 60.0%
! ACGTGGAGAAGTGGGGTGTC+CGG - Chr1:28016950-28016969 None:intergenic 60.0%
AGGAGCCGAGGTTCGAACCC+CGG + Chr1:28016928-28016947 MsG0180001839.01.T01:intron 65.0%
GGTGTCCGGGGTTCGAACCT+CGG - Chr1:28016936-28016955 None:intergenic 65.0%
! CGTGGAGAAGTGGGGTGTCC+GGG - Chr1:28016949-28016968 None:intergenic 65.0%
! GTGGAGAAGTGGGGTGTCCG+GGG - Chr1:28016948-28016967 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 28016632 28021053 28016632 ID=MsG0180001839.01;Name=MsG0180001839.01
Chr1 mRNA 28016632 28021053 28016632 ID=MsG0180001839.01.T01;Parent=MsG0180001839.01;Name=MsG0180001839.01.T01;_AED=0.77;_eAED=0.77;_QI=0|0|0|0.4|1|1|5|0|317
Chr1 exon 28016632 28016718 28016632 ID=MsG0180001839.01.T01:exon:18704;Parent=MsG0180001839.01.T01
Chr1 exon 28017522 28017576 28017522 ID=MsG0180001839.01.T01:exon:18705;Parent=MsG0180001839.01.T01
Chr1 exon 28018539 28018724 28018539 ID=MsG0180001839.01.T01:exon:18706;Parent=MsG0180001839.01.T01
Chr1 exon 28019124 28019305 28019124 ID=MsG0180001839.01.T01:exon:18707;Parent=MsG0180001839.01.T01
Chr1 exon 28020610 28021053 28020610 ID=MsG0180001839.01.T01:exon:18708;Parent=MsG0180001839.01.T01
Chr1 CDS 28016632 28016718 28016632 ID=MsG0180001839.01.T01:cds;Parent=MsG0180001839.01.T01
Chr1 CDS 28017522 28017576 28017522 ID=MsG0180001839.01.T01:cds;Parent=MsG0180001839.01.T01
Chr1 CDS 28018539 28018724 28018539 ID=MsG0180001839.01.T01:cds;Parent=MsG0180001839.01.T01
Chr1 CDS 28019124 28019305 28019124 ID=MsG0180001839.01.T01:cds;Parent=MsG0180001839.01.T01
Chr1 CDS 28020610 28021053 28020610 ID=MsG0180001839.01.T01:cds;Parent=MsG0180001839.01.T01
Gene Sequence

>MsG0180001839.01.T01

ATGCATAGGCTAACACTCGGCAAATGGTCATACGATTACAAGTGGCACTTGTCTATTGGTTTGGGCAATGTGGCCAACCTTTTTATGATTCCCTCTACTTGGGGAAATCATATTCAAAGTAGGGATGATCACATCCAAACTGAAACTAAACTTTGGCGTAAAGGACCGTATGAGAAGCCAATACTATGTAATGCTTGTGGACTACATTTCAAGGCTAAAGGAACCCTTGATAACTATCTTCCGAAGACATCCCAGCAAAATCAGTTATCAGACCTTGACATTGAAAAGGAATCTGAAAGTGATCCCGAGCTTTCAAACCAATCTCCAGAAAGGACACCATCGATTGTGAAGAATCAAATGGAGGAGTTTCAACAAAAGCTTCAAAGTCTATGGGAATCTGAGAAAGAATCAAATGAATCATCTGCAGACAATATTTTGTTGTTAGATAATATGAACAAATGTATACCAAGCAATGAGATAGGCATTGGATGCACTCTACTGAAGACAGATGTACCCAAACTAAAGAGGAAGATCAACATGGTGAGAACAAAGATAACAACAAGGAAAACAACTGGGGAAAAACCAATTGGAGGATTGAAGAAAGTTCCACAAGAGGAGATCCGACAAGTCAGCTTCATGGATGAGGTTCCAATCAAAATCAAAGACAAGAATGCTTTCAGAAGAAGGAACATATTTATGATCAAATCGCATGAACTCTTCTGTCCACCTACTCTCATGCATAAGTCTTGGTATAAGCCTTTCGAAATAACTCACATCTGCAAGAACAATCGTGTTCTGATCAAGGATCAAACTAGATGGTTGGCCAAGGTCCCAATTGAGGAAGTCATGCACATGCGAGACTATATGCAAAATCGAAGTAGTACAAGGGATGAACCAGATACACACATGTCTAAAAACAACACTTGGACATATCCGCCAGGGTTGGAACGCTAA
Protein sequence

>MsG0180001839.01.T01

MHRLTLGKWSYDYKWHLSIGLGNVANLFMIPSTWGNHIQSRDDHIQTETKLWRKGPYEKPILCNACGLHFKAKGTLDNYLPKTSQQNQLSDLDIEKESESDPELSNQSPERTPSIVKNQMEEFQQKLQSLWESEKESNESSADNILLLDNMNKCIPSNEIGIGCTLLKTDVPKLKRKINMVRTKITTRKTTGEKPIGGLKKVPQEEIRQVSFMDEVPIKIKDKNAFRRRNIFMIKSHELFCPPTLMHKSWYKPFEITHICKNNRVLIKDQTRWLAKVPIEEVMHMRDYMQNRSSTRDEPDTHMSKNNTWTYPPGLER*