AlfalfaGEDB | MsG0180002047.01

M. sativa cultivar ZhongmuNo.1 / MsG0180002047.01

KEGG: -
GO: GO:0003700 GO:0006355 GO:0008270 GO:0043565
PFAM: PF00320

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002047.01.T01	XP_003588383.1	74.349	269	48	5	1	249	1	268	3.41E-118	351

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002047.01.T01	P69781	41.729	266	125	9	1	249	1	253	3.85E-45	157

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002047.01.T01	G7IB47	74.349	269	48	5	1	249	1	268	1.63e-118	351

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TFs/TRs:

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Gene ID	Type	Classification
MsG0180002047.01.T01	TF	C2C2-GATA

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002047.01.T01	MTR_1g006650	74.349	269	48	5	1	249	1	268	4.13e-122	351
MsG0180002047.01.T01	MTR_3g109760	47.059	238	101	9	19	249	22	241	6.38e-55	179
MsG0180002047.01.T01	MTR_8g013950	41.530	183	73	5	78	249	101	260	1.86e-32	121
MsG0180002047.01.T01	MTR_8g076340	41.667	168	80	5	82	249	103	252	6.38e-26	103
MsG0180002047.01.T01	MTR_6g032830	33.333	198	97	7	86	249	30	226	7.07e-22	91.7
MsG0180002047.01.T01	MTR_5g021340	38.172	186	93	5	82	249	153	334	5.48e-21	91.3
MsG0180002047.01.T01	MTR_4g071590	33.929	168	74	6	82	249	115	245	3.80e-20	87.4
MsG0180002047.01.T01	MTR_4g471590	33.929	168	74	6	82	249	115	245	3.80e-20	87.4
MsG0180002047.01.T01	MTR_3g117380	31.361	169	98	3	82	249	112	263	1.35e-18	83.6
MsG0180002047.01.T01	MTR_1g094930	32.850	207	92	7	87	249	73	276	1.41e-17	80.9

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180002047.01.T01	AT5G25830	41.729	266	125	9	1	249	1	253	3.92e-46	157
MsG0180002047.01.T01	AT4G32890	39.837	246	101	8	21	249	16	231	3.26e-40	141
MsG0180002047.01.T01	AT2G45050	35.821	201	89	8	58	249	44	213	6.69e-21	89.0
MsG0180002047.01.T01	AT5G66320	35.821	201	90	6	75	249	96	283	2.62e-20	88.6
MsG0180002047.01.T01	AT5G66320	35.821	201	90	6	75	249	96	283	2.62e-20	88.6
MsG0180002047.01.T01	AT3G60530	37.870	169	61	6	82	249	67	192	4.31e-20	86.3
MsG0180002047.01.T01	AT4G34680	33.133	166	67	4	84	249	93	214	7.85e-19	83.6
MsG0180002047.01.T01	AT4G34680	33.133	166	67	4	84	249	93	214	7.85e-19	83.6
MsG0180002047.01.T01	AT4G36240	35.088	171	60	5	88	249	70	198	1.73e-18	82.0
MsG0180002047.01.T01	AT3G24050	80.556	36	7	0	214	249	193	228	1.95e-15	74.3

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Find 94 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACATTATGCAATGCTTG+TGG	0.626014	1:-31656291	MsG0180002047.01.T01:CDS
AAAACGATTCCTCACCAATA+TGG	0.265150	1:+31656709	None:intergenic
AAAACTTCCTTGTTGAATAT+AGG	0.362486	1:+31656588	None:intergenic
AAACAGTCCTATATTCAACA+AGG	0.707758	1:-31656595	MsG0180002047.01.T01:CDS
AAACGATTCCTCACCAATAT+GGG	0.454989	1:+31656710	None:intergenic
AAAGACTGCTCCAAGAAGAA+AGG	0.555772	1:-31656406	MsG0180002047.01.T01:CDS
AAATTCTGACAGCCAATCTC+AGG	0.491898	1:+31657426	None:intergenic
AACAGTGACGGTGGGTAAGG+TGG	0.408327	1:+31656443	None:intergenic
AACGACATTAGGGTCGCACC+CGG	0.603894	1:+31657449	None:intergenic
AACGCTATTGACGACGGTAA+CGG	0.582703	1:+31657488	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACATTATGCAATGCTTG+TGG	-	Chr1:31657646-31657665	MsG0180002047.01.T01:CDS	30.0%
AAACAGTCCTATATTCAACA+AGG	-	Chr1:31657342-31657361	MsG0180002047.01.T01:intron	30.0%
AAGGGTTCAAAATCTTCAAA+TGG	+	Chr1:31656880-31656899	None:intergenic	30.0%
CTCATCAACAATGAAATTGT+CGG	+	Chr1:31656330-31656349	None:intergenic	30.0%
CTGTCATTATTGGCAAAAAA+GGG	+	Chr1:31656898-31656917	None:intergenic	30.0%
TAGGCTAAATACAACAGAAT+GGG	-	Chr1:31656935-31656954	MsG0180002047.01.T01:intron	30.0%
TCAAAAGATGCAACTGATAT+CGG	-	Chr1:31657264-31657283	MsG0180002047.01.T01:intron	30.0%
TGTTGAATATAGGACTGTTT+CGG	+	Chr1:31657342-31657361	None:intergenic	30.0%
AAAACGATTCCTCACCAATA+TGG	+	Chr1:31657231-31657250	None:intergenic	35.0%
AAACGATTCCTCACCAATAT+GGG	+	Chr1:31657230-31657249	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	31656269	31657690	31656269	ID=MsG0180002047.01;Name=MsG0180002047.01
Chr1	mRNA	31656269	31657690	31656269	ID=MsG0180002047.01.T01;Parent=MsG0180002047.01;Name=MsG0180002047.01.T01;_AED=0.13;_eAED=0.29;_QI=0\|0\|0\|1\|1\|1\|2\|0\|249
Chr1	exon	31657436	31657690	31657436	ID=MsG0180002047.01.T01:exon:28534;Parent=MsG0180002047.01.T01
Chr1	exon	31656269	31656763	31656269	ID=MsG0180002047.01.T01:exon:28535;Parent=MsG0180002047.01.T01
Chr1	CDS	31657436	31657690	31657436	ID=MsG0180002047.01.T01:cds;Parent=MsG0180002047.01.T01
Chr1	CDS	31656269	31656763	31656269	ID=MsG0180002047.01.T01:cds;Parent=MsG0180002047.01.T01

Gene Sequence

>MsG0180002047.01.T01

ATGGAAGGCACAACCAACAACATTTTCCAAACCACCTTTTCCTCCGACAACAACACTACCGACAATTTCATTGTTGATGAGGACCTCCTTAAGCTCAACAATGACGTCGACGATGACGCTACATCATTTCGGACACCAACCTTGACTCGGCCACAGGCAACTCAACTGCCTCCTCCTCCACCGTTACCGTCGTCAATAGCGTTAGCTCGTCATCATTGTCCGGGTGCGACCCTAATGTCGTTCCTGAGATTGGCTGAGGATGATTTAGCGGACCTGGAATGGATTTCCCATATTGGTGAGGAATCGTTTTCAAGCGAGGATCTTCAAAAGATGCAACTGATATCGGGCATGAAGGTGCGAAACCAGGATGAAGAACCCCGCGAGCTTTCCCAACCCAACCGAAACAGTCCTATATTCAACAAGGAAGTTTTGGTTCCAGCTAAGGCCCGTAGCAAGAGGACTCGTGGGCCCCCTTGCGACTGGAGCTCGCGTCTTCTTGTTTTGTCTCAAACAACACCGTCTTCTTCTGAGTCAGAACTCCTTATTCCCACCCCCACCTTACCCACCGTCACTGTTCCAAGGAAGCAGGCAAAGACTGCTCCAAGAAGAAAGGACAGTGACGGAGGGTCAGGTGGAGATGGTCGGAGGTGTCGGCATTGCATGACAGACAAGACGCCACAATGGCGAACTGGGCCTAATGGCCCAAAAACATTATGCAATGCTTGTGGCGTAAGATATAAGTCTGGTTGA

Protein sequence

>MsG0180002047.01.T01

MEGTTNNIFQTTFSSDNNTTDNFIVDEDLLKLNNDVDDDATSFRTPTLTRPQATQLPPPPPLPSSIALARHHCPGATLMSFLRLAEDDLADLEWISHIGEESFSSEDLQKMQLISGMKVRNQDEEPRELSQPNRNSPIFNKEVLVPAKARSKRTRGPPCDWSSRLLVLSQTTPSSSESELLIPTPTLPTVTVPRKQAKTAPRRKDSDGGSGGDGRRCRHCMTDKTPQWRTGPNGPKTLCNACGVRYKSG*