Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002514.01.T01 | XP_013467327.1 | 97.714 | 175 | 4 | 0 | 1 | 175 | 59 | 233 | 1.15E-124 | 360 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002514.01.T01 | Q9ZV06 | 67.241 | 174 | 54 | 2 | 2 | 174 | 63 | 234 | 2.25E-80 | 241 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002514.01.T01 | B7FGG9 | 97.714 | 175 | 4 | 0 | 1 | 175 | 59 | 233 | 5.47e-125 | 360 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180002514.01.T01 | TR | GNAT |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002514.01.T01 | MTR_1g049370 | 97.714 | 175 | 4 | 0 | 1 | 175 | 59 | 233 | 1.39e-128 | 360 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002514.01.T01 | AT2G39020 | 67.241 | 174 | 54 | 2 | 2 | 174 | 63 | 234 | 2.29e-81 | 241 |
| MsG0180002514.01.T01 | AT2G39030 | 60.119 | 168 | 65 | 1 | 3 | 170 | 57 | 222 | 3.37e-75 | 225 |
Find 37 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGACTCACTGGAGAGAATT+TGG | 0.142625 | 1:-39604421 | MsG0180002514.01.T01:CDS |
| TCCTTCTCTGGGTCATCAAT+TGG | 0.238251 | 1:+39604732 | None:intergenic |
| CCTTCTCTGGGTCATCAATT+GGG | 0.321412 | 1:+39604733 | None:intergenic |
| GGAGAGAATTTGGAGGCTTA+TGG | 0.324071 | 1:-39604411 | MsG0180002514.01.T01:CDS |
| TGCGAAACAAGCTGTGAAAA+TGG | 0.330688 | 1:-39604544 | MsG0180002514.01.T01:CDS |
| TTTGTGATGGGTTTGTAAAA+TGG | 0.346829 | 1:+39604774 | None:intergenic |
| TTGAATGGGTTGTTCTTGAT+TGG | 0.376314 | 1:-39604509 | MsG0180002514.01.T01:CDS |
| GTTATAGAAGGAAAGGGTTT+GGG | 0.376869 | 1:-39604587 | MsG0180002514.01.T01:CDS |
| AAAATGGGTCGTTGTCGAAA+TGG | 0.382151 | 1:+39604790 | None:intergenic |
| TTGTGATGGGTTTGTAAAAT+GGG | 0.394107 | 1:+39604775 | None:intergenic |
| TGTTATAGAAGGAAAGGGTT+TGG | 0.394466 | 1:-39604588 | MsG0180002514.01.T01:CDS |
| GGAGAAGAGAGTGGTGGAGT+AGG | 0.423379 | 1:+39604884 | None:intergenic |
| AGAGAGTGTTATAGAAGGAA+AGG | 0.429394 | 1:-39604594 | MsG0180002514.01.T01:CDS |
| TTGTGAGAGAGTGTTATAGA+AGG | 0.433799 | 1:-39604599 | MsG0180002514.01.T01:CDS |
| GAGGATGGTGGTGGAATGGA+AGG | 0.442348 | 1:+39604848 | None:intergenic |
| AAATGGGTCGTTGTCGAAAT+GGG | 0.450614 | 1:+39604791 | None:intergenic |
| TGGAGGCTTTGCAGACTCAC+TGG | 0.463120 | 1:-39604432 | MsG0180002514.01.T01:CDS |
| GCGAAACAAGCTGTGAAAAT+GGG | 0.467825 | 1:-39604543 | MsG0180002514.01.T01:CDS |
| GGATGAGGATGGTGGTGGAA+TGG | 0.477208 | 1:+39604844 | None:intergenic |
| GAGAGTGTTATAGAAGGAAA+GGG | 0.477940 | 1:-39604593 | MsG0180002514.01.T01:CDS |
| TGGGGTATGGAAGAGTTGAA+TGG | 0.498138 | 1:-39604524 | MsG0180002514.01.T01:CDS |
| CGAAATGGGTATCGGTGAAA+GGG | 0.509100 | 1:+39604805 | None:intergenic |
| TTTGAGAGACTTCAAGGATG+AGG | 0.512897 | 1:+39604829 | None:intergenic |
| GGGGTATGGAAGAGTTGAAT+GGG | 0.513899 | 1:-39604523 | MsG0180002514.01.T01:CDS |
| TCGAAATGGGTATCGGTGAA+AGG | 0.517532 | 1:+39604804 | None:intergenic |
| CAAGCTGTGAAAATGGGGTA+TGG | 0.543716 | 1:-39604537 | MsG0180002514.01.T01:CDS |
| TTCAGGGGAGAAGAGAGTGG+TGG | 0.564694 | 1:+39604878 | None:intergenic |
| TTCAAGGATGAGGATGGTGG+TGG | 0.566001 | 1:+39604839 | None:intergenic |
| ACTCACTGGAGAGAATTTGG+AGG | 0.568925 | 1:-39604418 | MsG0180002514.01.T01:CDS |
| GTCGTTGTCGAAATGGGTAT+CGG | 0.570206 | 1:+39604797 | None:intergenic |
| CCCAATTGATGACCCAGAGA+AGG | 0.579350 | 1:-39604733 | MsG0180002514.01.T01:CDS |
| AGAGACTTCAAGGATGAGGA+TGG | 0.590756 | 1:+39604833 | None:intergenic |
| CGAAACAAGCTGTGAAAATG+GGG | 0.605125 | 1:-39604542 | MsG0180002514.01.T01:CDS |
| GACTTCAAGGATGAGGATGG+TGG | 0.644873 | 1:+39604836 | None:intergenic |
| AGGATGGTGGTGGAATGGAA+GGG | 0.647470 | 1:+39604849 | None:intergenic |
| GAAAACTTCAAAACTCAACA+TGG | 0.653987 | 1:-39604711 | MsG0180002514.01.T01:CDS |
| GGAGTAGGAAGCTTCTGTCG+CGG | 0.687951 | 1:+39604899 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAAAGTTTTATGAAGATAT+GGG | - | Chr1:39604827-39604846 | MsG0180002514.01.T01:CDS | 15.0% |
| !!! | TATAAAGTTTTATGAAGATA+TGG | - | Chr1:39604826-39604845 | MsG0180002514.01.T01:CDS | 15.0% |
| !! | AAGTTAACAGTTTTTGTGAT+GGG | + | Chr1:39604542-39604561 | None:intergenic | 25.0% |
| CATCACAAAAACTGTTAACT+TGG | - | Chr1:39604541-39604560 | MsG0180002514.01.T01:CDS | 30.0% | |
| GAAAACTTCAAAACTCAACA+TGG | - | Chr1:39604590-39604609 | MsG0180002514.01.T01:CDS | 30.0% | |
| ! | CAAGTTAACAGTTTTTGTGA+TGG | + | Chr1:39604543-39604562 | None:intergenic | 30.0% |
| !! | TTGTGATGGGTTTGTAAAAT+GGG | + | Chr1:39604529-39604548 | None:intergenic | 30.0% |
| !! | TTTGTGATGGGTTTGTAAAA+TGG | + | Chr1:39604530-39604549 | None:intergenic | 30.0% |
| !!! | CCTAACTACTCAACTTTTTT+GGG | - | Chr1:39604650-39604669 | MsG0180002514.01.T01:CDS | 30.0% |
| !!! | CTAACTACTCAACTTTTTTG+GGG | - | Chr1:39604651-39604670 | MsG0180002514.01.T01:CDS | 30.0% |
| !!! | TCCTAACTACTCAACTTTTT+TGG | - | Chr1:39604649-39604668 | MsG0180002514.01.T01:CDS | 30.0% |
| !!! | TTTTGAAGTTTTCCTTCTCT+GGG | + | Chr1:39604583-39604602 | None:intergenic | 30.0% |
| CCCAAAAAAGTTGAGTAGTT+AGG | + | Chr1:39604653-39604672 | None:intergenic | 35.0% | |
| GTTATAGAAGGAAAGGGTTT+GGG | - | Chr1:39604714-39604733 | MsG0180002514.01.T01:CDS | 35.0% | |
| TGTTATAGAAGGAAAGGGTT+TGG | - | Chr1:39604713-39604732 | MsG0180002514.01.T01:CDS | 35.0% | |
| ! | GAAATGAAGTTTTCGTTGCT+GGG | - | Chr1:39604612-39604631 | MsG0180002514.01.T01:CDS | 35.0% |
| !! | AATGGAAGGGTTTGTTTTCA+GGG | + | Chr1:39604442-39604461 | None:intergenic | 35.0% |
| !! | AGAGAGTGTTATAGAAGGAA+AGG | - | Chr1:39604707-39604726 | MsG0180002514.01.T01:CDS | 35.0% |
| !! | GAAACATGGGTTTTATGTTG+AGG | - | Chr1:39604673-39604692 | MsG0180002514.01.T01:CDS | 35.0% |
| !! | GAGAGTGTTATAGAAGGAAA+GGG | - | Chr1:39604708-39604727 | MsG0180002514.01.T01:CDS | 35.0% |
| !! | TTGAATGGGTTGTTCTTGAT+TGG | - | Chr1:39604792-39604811 | MsG0180002514.01.T01:CDS | 35.0% |
| !! | TTGTGAGAGAGTGTTATAGA+AGG | - | Chr1:39604702-39604721 | MsG0180002514.01.T01:CDS | 35.0% |
| !!! | CAACTTTTTTGGGGAAACAT+GGG | - | Chr1:39604660-39604679 | MsG0180002514.01.T01:CDS | 35.0% |
| !!! | GTTTTGAAGTTTTCCTTCTC+TGG | + | Chr1:39604584-39604603 | None:intergenic | 35.0% |
| !!! | TCAACTTTTTTGGGGAAACA+TGG | - | Chr1:39604659-39604678 | MsG0180002514.01.T01:CDS | 35.0% |
| AAAATGGGTCGTTGTCGAAA+TGG | + | Chr1:39604514-39604533 | None:intergenic | 40.0% | |
| AAATGGGTCGTTGTCGAAAT+GGG | + | Chr1:39604513-39604532 | None:intergenic | 40.0% | |
| CGAAACAAGCTGTGAAAATG+GGG | - | Chr1:39604759-39604778 | MsG0180002514.01.T01:CDS | 40.0% | |
| GCGAAACAAGCTGTGAAAAT+GGG | - | Chr1:39604758-39604777 | MsG0180002514.01.T01:CDS | 40.0% | |
| TGCGAAACAAGCTGTGAAAA+TGG | - | Chr1:39604757-39604776 | MsG0180002514.01.T01:CDS | 40.0% | |
| TTTGAGAGACTTCAAGGATG+AGG | + | Chr1:39604475-39604494 | None:intergenic | 40.0% | |
| ! | CTAAGATTTTGCAGGAATGG+AGG | - | Chr1:39604852-39604871 | MsG0180002514.01.T01:CDS | 40.0% |
| ! | GGAAATGAAGTTTTCGTTGC+TGG | - | Chr1:39604611-39604630 | MsG0180002514.01.T01:CDS | 40.0% |
| ! | GTGCTAAGATTTTGCAGGAA+TGG | - | Chr1:39604849-39604868 | MsG0180002514.01.T01:CDS | 40.0% |
| ! | TATGGGTGCTAAGATTTTGC+AGG | - | Chr1:39604844-39604863 | MsG0180002514.01.T01:CDS | 40.0% |
| !! | ATGGAAGGGTTTGTTTTCAG+GGG | + | Chr1:39604441-39604460 | None:intergenic | 40.0% |
| !! | GAATGGAAGGGTTTGTTTTC+AGG | + | Chr1:39604443-39604462 | None:intergenic | 40.0% |
| !!! | AAGGGTTTTGAGAGACTTCA+AGG | + | Chr1:39604481-39604500 | None:intergenic | 40.0% |
| ACTCACTGGAGAGAATTTGG+AGG | - | Chr1:39604883-39604902 | MsG0180002514.01.T01:CDS | 45.0% | |
| AGAGACTTCAAGGATGAGGA+TGG | + | Chr1:39604471-39604490 | None:intergenic | 45.0% | |
| CAAGCTGTGAAAATGGGGTA+TGG | - | Chr1:39604764-39604783 | MsG0180002514.01.T01:CDS | 45.0% | |
| CAGACTCACTGGAGAGAATT+TGG | - | Chr1:39604880-39604899 | MsG0180002514.01.T01:CDS | 45.0% | |
| CCTTCTCTGGGTCATCAATT+GGG | + | Chr1:39604571-39604590 | None:intergenic | 45.0% | |
| GGAGAGAATTTGGAGGCTTA+TGG | - | Chr1:39604890-39604909 | MsG0180002514.01.T01:CDS | 45.0% | |
| GGGGTATGGAAGAGTTGAAT+GGG | - | Chr1:39604778-39604797 | MsG0180002514.01.T01:CDS | 45.0% | |
| GTCGTTGTCGAAATGGGTAT+CGG | + | Chr1:39604507-39604526 | None:intergenic | 45.0% | |
| TCCTTCTCTGGGTCATCAAT+TGG | + | Chr1:39604572-39604591 | None:intergenic | 45.0% | |
| TGGGGTATGGAAGAGTTGAA+TGG | - | Chr1:39604777-39604796 | MsG0180002514.01.T01:CDS | 45.0% | |
| !! | CGAAATGGGTATCGGTGAAA+GGG | + | Chr1:39604499-39604518 | None:intergenic | 45.0% |
| !! | TCGAAATGGGTATCGGTGAA+AGG | + | Chr1:39604500-39604519 | None:intergenic | 45.0% |
| GACTTCAAGGATGAGGATGG+TGG | + | Chr1:39604468-39604487 | None:intergenic | 50.0% | |
| ! | CCCAATTGATGACCCAGAGA+AGG | - | Chr1:39604568-39604587 | MsG0180002514.01.T01:CDS | 50.0% |
| ! | TTCAAGGATGAGGATGGTGG+TGG | + | Chr1:39604465-39604484 | None:intergenic | 50.0% |
| !! | AGGATGGTGGTGGAATGGAA+GGG | + | Chr1:39604455-39604474 | None:intergenic | 50.0% |
| !! | GTTTTCAGGGGAGAAGAGAG+TGG | + | Chr1:39604429-39604448 | None:intergenic | 50.0% |
| GGAGTAGGAAGCTTCTGTCG+CGG | + | Chr1:39604405-39604424 | None:intergenic | 55.0% | |
| TGGAGGCTTTGCAGACTCAC+TGG | - | Chr1:39604869-39604888 | MsG0180002514.01.T01:CDS | 55.0% | |
| TTCAGGGGAGAAGAGAGTGG+TGG | + | Chr1:39604426-39604445 | None:intergenic | 55.0% | |
| ! | GGAGAAGAGAGTGGTGGAGT+AGG | + | Chr1:39604420-39604439 | None:intergenic | 55.0% |
| !! | GAGGATGGTGGTGGAATGGA+AGG | + | Chr1:39604456-39604475 | None:intergenic | 55.0% |
| !! | GGATGAGGATGGTGGTGGAA+TGG | + | Chr1:39604460-39604479 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 39604398 | 39604925 | 39604398 | ID=MsG0180002514.01;Name=MsG0180002514.01 |
| Chr1 | mRNA | 39604398 | 39604925 | 39604398 | ID=MsG0180002514.01.T01;Parent=MsG0180002514.01;Name=MsG0180002514.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|175 |
| Chr1 | exon | 39604398 | 39604925 | 39604398 | ID=MsG0180002514.01.T01:exon:8165;Parent=MsG0180002514.01.T01 |
| Chr1 | CDS | 39604398 | 39604925 | 39604398 | ID=MsG0180002514.01.T01:cds;Parent=MsG0180002514.01.T01 |
| Gene Sequence |
| Protein sequence |
