Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002582.01.T01 | KEH41323.1 | 97.561 | 82 | 2 | 0 | 34 | 115 | 11 | 92 | 5.64E-52 | 169 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002582.01.T01 | Q9LVS0 | 52.941 | 68 | 32 | 0 | 36 | 103 | 97 | 164 | 1.97E-19 | 84.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002582.01.T01 | A0A072VIR9 | 97.561 | 82 | 2 | 0 | 34 | 115 | 11 | 92 | 2.69e-52 | 169 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180002582.01.T01 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002582.01.T01 | MTR_1g048660 | 97.561 | 82 | 2 | 0 | 34 | 115 | 11 | 92 | 6.83e-56 | 169 |
| MsG0180002582.01.T01 | MTR_8g101650 | 54.321 | 81 | 35 | 2 | 36 | 115 | 106 | 185 | 2.02e-21 | 86.3 |
| MsG0180002582.01.T01 | MTR_5g088010 | 58.333 | 72 | 27 | 1 | 37 | 105 | 62 | 133 | 4.69e-21 | 84.7 |
| MsG0180002582.01.T01 | MTR_5g088060 | 58.333 | 72 | 27 | 1 | 37 | 105 | 274 | 345 | 1.64e-20 | 85.5 |
| MsG0180002582.01.T01 | MTR_5g488160 | 58.333 | 72 | 27 | 1 | 37 | 105 | 274 | 345 | 1.64e-20 | 85.5 |
| MsG0180002582.01.T01 | MTR_2g100930 | 54.688 | 64 | 29 | 0 | 36 | 99 | 110 | 173 | 2.14e-20 | 84.3 |
| MsG0180002582.01.T01 | MTR_6g092540 | 48.315 | 89 | 36 | 3 | 36 | 115 | 98 | 185 | 1.14e-19 | 81.6 |
| MsG0180002582.01.T01 | MTR_3g104370 | 71.739 | 46 | 13 | 0 | 36 | 81 | 92 | 137 | 4.73e-19 | 79.3 |
| MsG0180002582.01.T01 | MTR_7g067080 | 46.835 | 79 | 42 | 0 | 36 | 114 | 141 | 219 | 2.46e-18 | 78.6 |
| MsG0180002582.01.T01 | MTR_5g069710 | 54.688 | 64 | 29 | 0 | 36 | 99 | 133 | 196 | 3.99e-18 | 77.8 |
| MsG0180002582.01.T01 | MTR_3g462790 | 54.688 | 64 | 29 | 0 | 36 | 99 | 148 | 211 | 4.86e-18 | 77.8 |
| MsG0180002582.01.T01 | MTR_5g027570 | 51.429 | 70 | 32 | 1 | 37 | 104 | 94 | 163 | 1.01e-17 | 75.9 |
| MsG0180002582.01.T01 | MTR_4g100630 | 47.059 | 68 | 36 | 0 | 36 | 103 | 96 | 163 | 1.67e-17 | 76.3 |
| MsG0180002582.01.T01 | MTR_5g037080 | 56.250 | 64 | 28 | 0 | 36 | 99 | 103 | 166 | 4.02e-17 | 75.1 |
| MsG0180002582.01.T01 | MTR_4g111975 | 50.000 | 64 | 32 | 0 | 36 | 99 | 139 | 202 | 6.41e-17 | 74.7 |
| MsG0180002582.01.T01 | MTR_1g111830 | 51.562 | 64 | 31 | 0 | 36 | 99 | 125 | 188 | 6.94e-17 | 73.6 |
| MsG0180002582.01.T01 | MTR_2g090305 | 50.000 | 64 | 32 | 0 | 36 | 99 | 29 | 92 | 1.02e-16 | 72.0 |
| MsG0180002582.01.T01 | MTR_5g081860 | 53.030 | 66 | 29 | 1 | 36 | 99 | 126 | 191 | 1.22e-16 | 73.6 |
| MsG0180002582.01.T01 | MTR_0036s0260 | 50.000 | 64 | 32 | 0 | 36 | 99 | 29 | 92 | 2.92e-16 | 71.2 |
| MsG0180002582.01.T01 | MTR_4g107230 | 65.217 | 46 | 16 | 0 | 36 | 81 | 85 | 130 | 4.99e-16 | 72.0 |
| MsG0180002582.01.T01 | MTR_5g027550 | 50.000 | 72 | 33 | 1 | 37 | 105 | 79 | 150 | 5.42e-16 | 70.9 |
| MsG0180002582.01.T01 | MTR_8g063870 | 50.633 | 79 | 36 | 2 | 37 | 112 | 94 | 172 | 3.25e-15 | 69.7 |
| MsG0180002582.01.T01 | MTR_4g015130 | 50.000 | 66 | 30 | 1 | 45 | 107 | 3 | 68 | 2.37e-14 | 63.5 |
| MsG0180002582.01.T01 | MTR_8g063600 | 45.455 | 77 | 38 | 2 | 36 | 109 | 107 | 182 | 2.38e-13 | 64.7 |
| MsG0180002582.01.T01 | MTR_3g070110 | 65.909 | 44 | 15 | 0 | 37 | 80 | 97 | 140 | 6.56e-13 | 62.8 |
| MsG0180002582.01.T01 | MTR_5g075760 | 58.824 | 51 | 20 | 1 | 31 | 81 | 101 | 150 | 2.10e-12 | 61.2 |
| MsG0180002582.01.T01 | MTR_6g043480 | 46.429 | 56 | 30 | 0 | 33 | 88 | 105 | 160 | 1.95e-11 | 58.9 |
| MsG0180002582.01.T01 | MTR_6g043490 | 38.462 | 78 | 48 | 0 | 33 | 110 | 105 | 182 | 2.71e-11 | 58.5 |
| MsG0180002582.01.T01 | MTR_5g075790 | 54.902 | 51 | 22 | 1 | 31 | 81 | 109 | 158 | 7.12e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002582.01.T01 | AT5G56840 | 55.128 | 78 | 30 | 2 | 36 | 108 | 92 | 169 | 6.00e-21 | 84.3 |
| MsG0180002582.01.T01 | AT5G47390 | 52.941 | 68 | 32 | 0 | 36 | 103 | 97 | 164 | 2.01e-20 | 84.7 |
| MsG0180002582.01.T01 | AT5G61620 | 55.072 | 69 | 31 | 0 | 35 | 103 | 108 | 176 | 7.13e-19 | 80.1 |
| MsG0180002582.01.T01 | AT5G58900 | 53.521 | 71 | 30 | 1 | 36 | 103 | 142 | 212 | 1.14e-18 | 79.0 |
| MsG0180002582.01.T01 | AT3G11280 | 53.125 | 64 | 30 | 0 | 36 | 99 | 128 | 191 | 7.53e-18 | 76.6 |
| MsG0180002582.01.T01 | AT3G11280 | 53.125 | 64 | 30 | 0 | 36 | 99 | 128 | 191 | 7.53e-18 | 76.6 |
| MsG0180002582.01.T01 | AT1G49010 | 45.455 | 77 | 42 | 0 | 36 | 112 | 135 | 211 | 7.99e-18 | 77.0 |
| MsG0180002582.01.T01 | AT5G04760 | 53.125 | 64 | 30 | 0 | 36 | 99 | 100 | 163 | 8.20e-18 | 75.5 |
| MsG0180002582.01.T01 | AT2G38090 | 53.125 | 64 | 30 | 0 | 36 | 99 | 142 | 205 | 9.84e-18 | 76.6 |
| MsG0180002582.01.T01 | AT5G01200 | 54.688 | 64 | 29 | 0 | 36 | 99 | 147 | 210 | 1.31e-17 | 75.9 |
| MsG0180002582.01.T01 | AT3G16350 | 51.471 | 68 | 33 | 0 | 36 | 103 | 138 | 205 | 4.49e-17 | 75.5 |
| MsG0180002582.01.T01 | AT1G70000 | 56.250 | 64 | 28 | 0 | 36 | 99 | 99 | 162 | 5.57e-17 | 74.3 |
| MsG0180002582.01.T01 | AT1G70000 | 56.250 | 64 | 28 | 0 | 36 | 99 | 99 | 162 | 5.57e-17 | 74.3 |
| MsG0180002582.01.T01 | AT1G19000 | 55.224 | 67 | 30 | 0 | 36 | 102 | 103 | 169 | 7.69e-17 | 74.3 |
| MsG0180002582.01.T01 | AT1G19000 | 55.224 | 67 | 30 | 0 | 36 | 102 | 103 | 169 | 7.69e-17 | 74.3 |
| MsG0180002582.01.T01 | AT5G05790 | 51.562 | 64 | 31 | 0 | 36 | 99 | 132 | 195 | 8.78e-17 | 73.9 |
| MsG0180002582.01.T01 | AT5G05790 | 51.562 | 64 | 31 | 0 | 36 | 99 | 132 | 195 | 8.78e-17 | 73.9 |
| MsG0180002582.01.T01 | AT5G08520 | 52.381 | 63 | 30 | 0 | 37 | 99 | 120 | 182 | 2.85e-16 | 72.8 |
| MsG0180002582.01.T01 | AT1G74840 | 57.812 | 64 | 27 | 0 | 36 | 99 | 97 | 160 | 4.13e-16 | 71.6 |
| MsG0180002582.01.T01 | AT1G74840 | 57.812 | 64 | 27 | 0 | 36 | 99 | 97 | 160 | 5.29e-16 | 71.6 |
| MsG0180002582.01.T01 | AT5G23650 | 49.275 | 69 | 35 | 0 | 36 | 104 | 121 | 189 | 1.03e-15 | 71.6 |
| MsG0180002582.01.T01 | AT4G09450 | 48.485 | 66 | 34 | 0 | 36 | 101 | 92 | 157 | 7.99e-15 | 67.4 |
| MsG0180002582.01.T01 | AT3G10580 | 46.479 | 71 | 36 | 1 | 36 | 106 | 96 | 164 | 1.73e-14 | 67.4 |
| MsG0180002582.01.T01 | AT3G10580 | 46.479 | 71 | 36 | 1 | 36 | 106 | 96 | 164 | 2.39e-14 | 67.4 |
| MsG0180002582.01.T01 | AT3G10590 | 46.154 | 78 | 37 | 2 | 36 | 111 | 112 | 186 | 4.80e-14 | 65.5 |
Find 18 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCTTTCTTGAAAGGTTTAA+AGG | 0.205247 | 1:+40796497 | MsG0180002582.01.T01:CDS |
| AGCAACCTGAGTAGGAGTTT+TGG | 0.403614 | 1:-40796568 | None:intergenic |
| GGTTTAAAGGCTGTTGGAAA+AGG | 0.435483 | 1:+40796510 | MsG0180002582.01.T01:CDS |
| TTGAAAGGTTTAAAGGCTGT+TGG | 0.453203 | 1:+40796504 | MsG0180002582.01.T01:CDS |
| TGGTGCTCCGTCCTTGTCCA+TGG | 0.462848 | 1:-40796474 | None:intergenic |
| TTAAACCTTTCAAGAAAGCC+TGG | 0.468661 | 1:-40796494 | None:intergenic |
| GAGCACCAGGCTTTCTTGAA+AGG | 0.474105 | 1:+40796489 | MsG0180002582.01.T01:CDS |
| CGTAATCAATATCTTACGCT+TGG | 0.481822 | 1:+40796393 | MsG0180002582.01.T01:CDS |
| ATGGACAAGGACGGAGCACC+AGG | 0.487460 | 1:+40796476 | MsG0180002582.01.T01:CDS |
| AATGATAATTCAAAAGCTAC+TGG | 0.495544 | 1:+40796846 | MsG0180002582.01.T01:CDS |
| AGGCTGTTGGAAAAGGCAAA+TGG | 0.496236 | 1:+40796517 | MsG0180002582.01.T01:CDS |
| GGTGACCAAAACTCCTACTC+AGG | 0.558451 | 1:+40796563 | MsG0180002582.01.T01:CDS |
| CTGGTGCTTCATCTGTATAG+AGG | 0.606898 | 1:+40796865 | MsG0180002582.01.T01:CDS |
| CAAATGGAAGCGAATATCGA+AGG | 0.631038 | 1:+40796533 | MsG0180002582.01.T01:CDS |
| GTTGCATTGTAGTGAAACCA+TGG | 0.639856 | 1:+40796457 | MsG0180002582.01.T01:CDS |
| GCATGACTAGCAACCTGAGT+AGG | 0.641227 | 1:-40796576 | None:intergenic |
| AGTGAAACCATGGACAAGGA+CGG | 0.664978 | 1:+40796467 | MsG0180002582.01.T01:CDS |
| TTGTAGTGAAACCATGGACA+AGG | 0.674968 | 1:+40796463 | MsG0180002582.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATGACTATATTGCTTGATA+TGG | + | Chr1:40796814-40796833 | MsG0180002582.01.T01:intron | 25.0% |
| ! | AATGATAATTCAAAAGCTAC+TGG | + | Chr1:40796846-40796865 | MsG0180002582.01.T01:CDS | 25.0% |
| ! | TTTGTACTAAAATTCAACTG+CGG | - | Chr1:40796757-40796776 | None:intergenic | 25.0% |
| !!! | AAGCATTTTAATTATAGACG+TGG | + | Chr1:40796603-40796622 | MsG0180002582.01.T01:CDS | 25.0% |
| !!! | CTGATTGAGATTTTTGTTAT+AGG | + | Chr1:40796700-40796719 | MsG0180002582.01.T01:intron | 25.0% |
| AAATCTCAATCAGAAAGAGA+AGG | - | Chr1:40796693-40796712 | None:intergenic | 30.0% | |
| ACATAATGTACGTATAACGA+AGG | - | Chr1:40796787-40796806 | None:intergenic | 30.0% | |
| ACTATATTGCTTGATATGGC+AGG | + | Chr1:40796818-40796837 | MsG0180002582.01.T01:intron | 35.0% | |
| CGTAATCAATATCTTACGCT+TGG | + | Chr1:40796393-40796412 | MsG0180002582.01.T01:CDS | 35.0% | |
| GGCTTTCTTGAAAGGTTTAA+AGG | + | Chr1:40796497-40796516 | MsG0180002582.01.T01:CDS | 35.0% | |
| TTAAACCTTTCAAGAAAGCC+TGG | - | Chr1:40796497-40796516 | None:intergenic | 35.0% | |
| TTGAAAGGTTTAAAGGCTGT+TGG | + | Chr1:40796504-40796523 | MsG0180002582.01.T01:CDS | 35.0% | |
| AAATTCAACTGCGGCCTATT+TGG | - | Chr1:40796748-40796767 | None:intergenic | 40.0% | |
| CAAATGGAAGCGAATATCGA+AGG | + | Chr1:40796533-40796552 | MsG0180002582.01.T01:CDS | 40.0% | |
| GGTTTAAAGGCTGTTGGAAA+AGG | + | Chr1:40796510-40796529 | MsG0180002582.01.T01:CDS | 40.0% | |
| GTTGCATTGTAGTGAAACCA+TGG | + | Chr1:40796457-40796476 | MsG0180002582.01.T01:CDS | 40.0% | |
| TTGTAGTGAAACCATGGACA+AGG | + | Chr1:40796463-40796482 | MsG0180002582.01.T01:CDS | 40.0% | |
| ! | GCGAATATCGAAGGATTTTG+TGG | + | Chr1:40796542-40796561 | MsG0180002582.01.T01:CDS | 40.0% |
| AGGCTGTTGGAAAAGGCAAA+TGG | + | Chr1:40796517-40796536 | MsG0180002582.01.T01:CDS | 45.0% | |
| AGTGAAACCATGGACAAGGA+CGG | + | Chr1:40796467-40796486 | MsG0180002582.01.T01:CDS | 45.0% | |
| TGTGTCTAGTCCCTCCAAAT+AGG | + | Chr1:40796731-40796750 | MsG0180002582.01.T01:intron | 45.0% | |
| ! | AGCAACCTGAGTAGGAGTTT+TGG | - | Chr1:40796571-40796590 | None:intergenic | 45.0% |
| GAGCACCAGGCTTTCTTGAA+AGG | + | Chr1:40796489-40796508 | MsG0180002582.01.T01:CDS | 50.0% | |
| GCATGACTAGCAACCTGAGT+AGG | - | Chr1:40796579-40796598 | None:intergenic | 50.0% | |
| GGTGACCAAAACTCCTACTC+AGG | + | Chr1:40796563-40796582 | MsG0180002582.01.T01:CDS | 50.0% | |
| TCAACTGCGGCCTATTTGGA+GGG | - | Chr1:40796744-40796763 | None:intergenic | 50.0% | |
| TTCAACTGCGGCCTATTTGG+AGG | - | Chr1:40796745-40796764 | None:intergenic | 50.0% | |
| TGGTGCTCCGTCCTTGTCCA+TGG | - | Chr1:40796477-40796496 | None:intergenic | 60.0% | |
| ! | ATGGACAAGGACGGAGCACC+AGG | + | Chr1:40796476-40796495 | MsG0180002582.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 40796369 | 40796884 | 40796369 | ID=MsG0180002582.01;Name=MsG0180002582.01 |
| Chr1 | mRNA | 40796369 | 40796884 | 40796369 | ID=MsG0180002582.01.T01;Parent=MsG0180002582.01;Name=MsG0180002582.01.T01;_AED=0.50;_eAED=0.74;_QI=0|0|0|1|0|0|2|0|115 |
| Chr1 | exon | 40796369 | 40796671 | 40796369 | ID=MsG0180002582.01.T01:exon:8378;Parent=MsG0180002582.01.T01 |
| Chr1 | exon | 40796840 | 40796884 | 40796840 | ID=MsG0180002582.01.T01:exon:8379;Parent=MsG0180002582.01.T01 |
| Chr1 | CDS | 40796369 | 40796671 | 40796369 | ID=MsG0180002582.01.T01:cds;Parent=MsG0180002582.01.T01 |
| Chr1 | CDS | 40796840 | 40796884 | 40796840 | ID=MsG0180002582.01.T01:cds;Parent=MsG0180002582.01.T01 |
| Gene Sequence |
| Protein sequence |
