AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002956.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002956.01.T01 MTR_1g053575 85.586 333 36 3 1 331 1 323 0.0 553
MsG0180002956.01.T01 MTR_1g045470 56.410 351 105 11 1 330 1 324 3.75e-112 328
MsG0180002956.01.T01 MTR_7g116460 59.615 208 51 6 1 183 1 200 2.38e-75 231
MsG0180002956.01.T01 MTR_1g019820 35.345 232 108 5 102 317 5 210 2.32e-36 130
MsG0180002956.01.T01 MTR_7g005280 32.143 168 91 9 11 170 19 171 3.66e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 59 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAAAGAATCCTTGGGATATT+TGG 0.040443 1:-53764828 MsG0180002956.01.T01:CDS
CCACCATTTGATTCGTTTCT+TGG 0.076491 1:+53764104 None:intergenic
TAACTCCTCATGAAATTTAT+TGG 0.168081 1:+53763056 None:intergenic
TACAAATTTGTTGGCTTCTT+CGG 0.206819 1:+53763983 None:intergenic
ATTTCATGAGGAGTTATTAT+TGG 0.237534 1:-53763049 MsG0180002956.01.T01:CDS
AAATATCCCAAGGATTCTTT+AGG 0.262491 1:+53764830 None:intergenic
TCATTGTCAGGTTGAACTAT+TGG 0.266404 1:+53763246 None:intergenic
TCAAGAATGGCGATATAATT+TGG 0.272332 1:+53764871 None:intergenic
ACAAATTTGTTGGCTTCTTC+GGG 0.289766 1:+53763984 None:intergenic
AGTGGTAGATTTCTTCTCTA+AGG 0.296212 1:+53764744 None:intergenic
CAGGTTGAACTATTGGATTT+TGG 0.311938 1:+53763253 None:intergenic
AACTCCTCATGAAATTTATT+GGG 0.319423 1:+53763057 None:intergenic
TCAAAAGTAGTTCGTTCTAT+TGG 0.321604 1:-53764718 MsG0180002956.01.T01:CDS
AAAGAATCCTTGGGATATTT+GGG 0.338135 1:-53764827 MsG0180002956.01.T01:CDS
TGGGATATTTGGGAAGAGTT+TGG 0.341365 1:-53764817 MsG0180002956.01.T01:CDS
AATTTGGCAGAGATTTGTTA+TGG 0.353680 1:+53764887 None:intergenic
TAATACAAAGTCTCATTATA+AGG 0.356324 1:-53764060 MsG0180002956.01.T01:CDS
GTAGTTCGTTCTATTGGAAT+TGG 0.365191 1:-53764712 MsG0180002956.01.T01:CDS
ATCTTTGATAAGAAATCCTT+TGG 0.365896 1:-53764542 MsG0180002956.01.T01:intron
TCTCAATTCTTCTTCAAGTA+AGG 0.366602 1:-53764965 MsG0180002956.01.T01:CDS
AATATCCCAAGGATTCTTTA+GGG 0.371252 1:+53764831 None:intergenic
CTAAGAATGGTTGTCTCTCT+TGG 0.381735 1:+53764008 None:intergenic
ATTCTTGAATACGATTTGTT+TGG 0.392427 1:-53764856 MsG0180002956.01.T01:CDS
TGGACACAATAGCACAAATA+TGG 0.393645 1:-53764084 MsG0180002956.01.T01:CDS
TGTAGGACTTACAAATTTGT+TGG 0.396611 1:+53763974 None:intergenic
TTAAGGTAGTGAAGAAATAT+AGG 0.411633 1:+53764767 None:intergenic
GGCTTCTTCGGGTCTAAGAA+TGG 0.413104 1:+53763995 None:intergenic
GAAAGAAAGACCATTGTTCC+TGG 0.417974 1:-53764381 MsG0180002956.01.T01:CDS
TTGGTACCCTAAAGAATCCT+TGG 0.424102 1:-53764837 MsG0180002956.01.T01:CDS
TCATCATCATCTTCATTGTC+AGG 0.425138 1:+53763234 None:intergenic
AATCAAGTAACTTACAGATA+TGG 0.426262 1:+53764344 None:intergenic
AGATTTCTTCTCTAAGGTTA+AGG 0.432842 1:+53764750 None:intergenic
TGGTACCCTAAAGAATCCTT+GGG 0.489046 1:-53764836 MsG0180002956.01.T01:CDS
ATGCTTGTTAGAAGAGGAAG+AGG 0.495031 1:-53763091 MsG0180002956.01.T01:CDS
GAACCAAGAAACGAATCAAA+TGG 0.503929 1:-53764107 MsG0180002956.01.T01:CDS
GTTCTATTGGAATTGGTGCA+TGG 0.508402 1:-53764705 MsG0180002956.01.T01:CDS
AAACAAATCGTATTCAAGAA+TGG 0.511320 1:+53764858 None:intergenic
TTCTTATCAAAGATTTGTCA+AGG 0.536486 1:+53764551 None:intergenic
GAGCTGATAGAGATGAATGA+AGG 0.539230 1:-53763114 MsG0180002956.01.T01:CDS
TGAAGGATGCTTGTTAGAAG+AGG 0.541017 1:-53763097 MsG0180002956.01.T01:CDS
ATGGTGGTTTCTGCACGTCC+AGG 0.545124 1:+53764363 None:intergenic
TTCTATTGGAATTGGTGCAT+GGG 0.546914 1:-53764704 MsG0180002956.01.T01:CDS
GCAAGATCCATGCACCATCA+TGG 0.566204 1:+53764584 None:intergenic
AACTCTTCCCAAATATCCCA+AGG 0.585203 1:+53764820 None:intergenic
CAAGTAACTTACAGATATGG+TGG 0.592493 1:+53764347 None:intergenic
TTCTGCACGTCCAGGAACAA+TGG 0.595867 1:+53764371 None:intergenic
ATGATGATGCTGGTACGTCT+TGG 0.605069 1:-53763155 MsG0180002956.01.T01:CDS
GACAATGAAGATGATGATGA+TGG 0.618553 1:-53763231 MsG0180002956.01.T01:CDS
GACGAAGACGATGATGATGC+TGG 0.627445 1:-53763165 MsG0180002956.01.T01:CDS
GATTCCCAATAAATTTCATG+AGG 0.630005 1:-53763061 MsG0180002956.01.T01:CDS
ATTGGAATTGGTGCATGGGA+AGG 0.630255 1:-53764700 MsG0180002956.01.T01:CDS
GCACTTACCATGATGGTGCA+TGG 0.633065 1:-53764591 MsG0180002956.01.T01:CDS
AATAGTGGCACTTACCATGA+TGG 0.645072 1:-53764598 MsG0180002956.01.T01:CDS
AGAAAGAATATTGATGCACA+AGG 0.650579 1:-53764408 MsG0180002956.01.T01:CDS
TGGGAAGGTGAAGAAGACAC+TGG 0.669846 1:-53764685 MsG0180002956.01.T01:CDS
CCAAGAAACGAATCAAATGG+TGG 0.672770 1:-53764104 MsG0180002956.01.T01:CDS
TGATGATGGTGAATGCATTG+TGG 0.673578 1:-53763217 MsG0180002956.01.T01:CDS
AGATGATGGTGAAAGCATTG+CGG 0.694760 1:-53763187 MsG0180002956.01.T01:CDS
ATTGTGGACAAAGAAGATGA+TGG 0.732612 1:-53763201 MsG0180002956.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATTAGTTATTAAATTTTT+TGG - Chr1:53763720-53763739 MsG0180002956.01.T01:intron 10.0%
!!! AAATTGGTAAATTTTAACTT+TGG + Chr1:53764420-53764439 None:intergenic 15.0%
!!! AATTGGTAAATTTTAACTTT+GGG + Chr1:53764419-53764438 None:intergenic 15.0%
!!! CATTTTTAAGTATTCAATAA+TGG - Chr1:53764257-53764276 MsG0180002956.01.T01:intron 15.0%
!!! CCTATTTTTATCTTATTTTT+GGG - Chr1:53764285-53764304 MsG0180002956.01.T01:intron 15.0%
!!! GATTTTGTATCTTTTTTTTT+AGG - Chr1:53763542-53763561 MsG0180002956.01.T01:intron 15.0%
!!! TCAAATGTTTTTCATAAAAT+GGG + Chr1:53764466-53764485 None:intergenic 15.0%
!!! TCCTATTTTTATCTTATTTT+TGG - Chr1:53764284-53764303 MsG0180002956.01.T01:intron 15.0%
!!! TTCAAATGTTTTTCATAAAA+TGG + Chr1:53764467-53764486 None:intergenic 15.0%
!! AAAAAGATGTGAGAAATATT+TGG - Chr1:53763968-53763987 MsG0180002956.01.T01:intron 20.0%
!! AAAGTGATGTATATTCAAAA+TGG - Chr1:53763756-53763775 MsG0180002956.01.T01:intron 20.0%
!! AACATATTAACACAATTATC+TGG - Chr1:53764164-53764183 MsG0180002956.01.T01:intron 20.0%
!! ATTGATCATATGTAATGTAA+TGG - Chr1:53764075-53764094 MsG0180002956.01.T01:CDS 20.0%
!! CCCAAAAATAAGATAAAAAT+AGG + Chr1:53764288-53764307 None:intergenic 20.0%
!! TAATACAAAGTCTCATTATA+AGG - Chr1:53763939-53763958 MsG0180002956.01.T01:intron 20.0%
!! TATTATACAACAACAAATGT+AGG + Chr1:53764045-53764064 None:intergenic 20.0%
!!! AACTTCACATTTTTTTTTTC+TGG - Chr1:53764535-53764554 MsG0180002956.01.T01:intron 20.0%
!!! ACTTCACATTTTTTTTTTCT+GGG - Chr1:53764536-53764555 MsG0180002956.01.T01:intron 20.0%
! AAACAAATCGTATTCAAGAA+TGG + Chr1:53763144-53763163 None:intergenic 25.0%
! AACTCCTCATGAAATTTATT+GGG + Chr1:53764945-53764964 None:intergenic 25.0%
! AATCAAGTAACTTACAGATA+TGG + Chr1:53763658-53763677 None:intergenic 25.0%
! AATGATAGTAACATACTAAC+TGG + Chr1:53764241-53764260 None:intergenic 25.0%
! AATTAGAATGCAAAATGAGT+TGG + Chr1:53764666-53764685 None:intergenic 25.0%
! ATCTTTGATAAGAAATCCTT+TGG - Chr1:53763457-53763476 MsG0180002956.01.T01:intron 25.0%
! ATGTTAAAGAGAAAAACAAC+TGG + Chr1:53763796-53763815 None:intergenic 25.0%
! ATTGACCATGAAAAAAGTTA+AGG + Chr1:53764491-53764510 None:intergenic 25.0%
! ATTTCATGAGGAGTTATTAT+TGG - Chr1:53764950-53764969 MsG0180002956.01.T01:CDS 25.0%
! TAACTCCTCATGAAATTTAT+TGG + Chr1:53764946-53764965 None:intergenic 25.0%
! TTAAATCACATCTATGTCAA+AGG - Chr1:53764594-53764613 MsG0180002956.01.T01:CDS 25.0%
! TTAAGGTAGTGAAGAAATAT+AGG + Chr1:53763235-53763254 None:intergenic 25.0%
! TTCTTATCAAAGATTTGTCA+AGG + Chr1:53763451-53763470 None:intergenic 25.0%
!! ATTCTTGAATACGATTTGTT+TGG - Chr1:53763143-53763162 MsG0180002956.01.T01:CDS 25.0%
!! TCTGCATAGAACATAATTTT+TGG + Chr1:53763568-53763587 None:intergenic 25.0%
!! TCTTTTCATCTCAGAAAAAT+TGG + Chr1:53764436-53764455 None:intergenic 25.0%
!!! TAACTTTTTTCATGGTCAAT+AGG - Chr1:53764491-53764510 MsG0180002956.01.T01:intron 25.0%
!!! TGAATCCTTAACTTTTTTCA+TGG - Chr1:53764483-53764502 MsG0180002956.01.T01:intron 25.0%
AAAGAATCCTTGGGATATTT+GGG - Chr1:53763172-53763191 MsG0180002956.01.T01:CDS 30.0%
AAATATCCCAAGGATTCTTT+AGG + Chr1:53763172-53763191 None:intergenic 30.0%
AATATCCCAAGGATTCTTTA+GGG + Chr1:53763171-53763190 None:intergenic 30.0%
AGAAAGAATATTGATGCACA+AGG - Chr1:53763591-53763610 MsG0180002956.01.T01:intron 30.0%
GATTCCCAATAAATTTCATG+AGG - Chr1:53764938-53764957 MsG0180002956.01.T01:CDS 30.0%
TAAAGAATCCTTGGGATATT+TGG - Chr1:53763171-53763190 MsG0180002956.01.T01:CDS 30.0%
TCAAAAGTAGTTCGTTCTAT+TGG - Chr1:53763281-53763300 MsG0180002956.01.T01:intron 30.0%
TCAAGAATGGCGATATAATT+TGG + Chr1:53763131-53763150 None:intergenic 30.0%
TCTCAATTCTTCTTCAAGTA+AGG - Chr1:53763034-53763053 MsG0180002956.01.T01:CDS 30.0%
TGTAGGACTTACAAATTTGT+TGG + Chr1:53764028-53764047 None:intergenic 30.0%
TGTATCATATTGCTTACCAA+AGG + Chr1:53763476-53763495 None:intergenic 30.0%
! CATTCTTGATTTGATATGCA+GGG - Chr1:53763826-53763845 MsG0180002956.01.T01:intron 30.0%
!! AATTTGGCAGAGATTTGTTA+TGG + Chr1:53763115-53763134 None:intergenic 30.0%
!! AGATTTCTTCTCTAAGGTTA+AGG + Chr1:53763252-53763271 None:intergenic 30.0%
!! TACAAATTTGTTGGCTTCTT+CGG + Chr1:53764019-53764038 None:intergenic 30.0%
!! TGTTATGGATTTTGTTGTAG+AGG + Chr1:53763100-53763119 None:intergenic 30.0%
!! TGTTTTCGATTTGAGAATAG+TGG - Chr1:53763386-53763405 MsG0180002956.01.T01:intron 30.0%
!!! CTCTTGCATTCTCATTTTTT+AGG - Chr1:53764713-53764732 MsG0180002956.01.T01:CDS 30.0%
AAAAACAAGAACCAACTCCT+TGG - Chr1:53763353-53763372 MsG0180002956.01.T01:intron 35.0%
AGTGGTAGATTTCTTCTCTA+AGG + Chr1:53763258-53763277 None:intergenic 35.0%
ATTGTGGACAAAGAAGATGA+TGG - Chr1:53764798-53764817 MsG0180002956.01.T01:CDS 35.0%
CAAGTAACTTACAGATATGG+TGG + Chr1:53763655-53763674 None:intergenic 35.0%
GAACCAAGAAACGAATCAAA+TGG - Chr1:53763892-53763911 MsG0180002956.01.T01:intron 35.0%
GTAGTTCGTTCTATTGGAAT+TGG - Chr1:53763287-53763306 MsG0180002956.01.T01:intron 35.0%
TCATCATCATCTTCATTGTC+AGG + Chr1:53764768-53764787 None:intergenic 35.0%
TGGACACAATAGCACAAATA+TGG - Chr1:53763915-53763934 MsG0180002956.01.T01:intron 35.0%
TTCTATTGGAATTGGTGCAT+GGG - Chr1:53763295-53763314 MsG0180002956.01.T01:intron 35.0%
! AGAATGCAAGAGCACAATAA+GGG + Chr1:53764705-53764724 None:intergenic 35.0%
! GACAATGAAGATGATGATGA+TGG - Chr1:53764768-53764787 MsG0180002956.01.T01:CDS 35.0%
! GCATTCTTGATTTGATATGC+AGG - Chr1:53763825-53763844 MsG0180002956.01.T01:intron 35.0%
!! ACAAATTTGTTGGCTTCTTC+GGG + Chr1:53764018-53764037 None:intergenic 35.0%
!! CAGGTTGAACTATTGGATTT+TGG + Chr1:53764749-53764768 None:intergenic 35.0%
!! TCATTGTCAGGTTGAACTAT+TGG + Chr1:53764756-53764775 None:intergenic 35.0%
!!! GAAAACACTTTTTCACACCA+AGG + Chr1:53763373-53763392 None:intergenic 35.0%
AACTCTTCCCAAATATCCCA+AGG + Chr1:53763182-53763201 None:intergenic 40.0%
AATAGTGGCACTTACCATGA+TGG - Chr1:53763401-53763420 MsG0180002956.01.T01:intron 40.0%
AGCTCCTTTGCAAAAATCTC+TGG + Chr1:53764870-53764889 None:intergenic 40.0%
CCAAGAAACGAATCAAATGG+TGG - Chr1:53763895-53763914 MsG0180002956.01.T01:intron 40.0%
CTAAGAATGGTTGTCTCTCT+TGG + Chr1:53763994-53764013 None:intergenic 40.0%
GAAAGAAAGACCATTGTTCC+TGG - Chr1:53763618-53763637 MsG0180002956.01.T01:intron 40.0%
GAGCTGATAGAGATGAATGA+AGG - Chr1:53764885-53764904 MsG0180002956.01.T01:CDS 40.0%
GTTCTATTGGAATTGGTGCA+TGG - Chr1:53763294-53763313 MsG0180002956.01.T01:intron 40.0%
TGGGATATTTGGGAAGAGTT+TGG - Chr1:53763182-53763201 MsG0180002956.01.T01:CDS 40.0%
TGGTACCCTAAAGAATCCTT+GGG - Chr1:53763163-53763182 MsG0180002956.01.T01:CDS 40.0%
TTGGTACCCTAAAGAATCCT+TGG - Chr1:53763162-53763181 MsG0180002956.01.T01:CDS 40.0%
! ATGCTTGTTAGAAGAGGAAG+AGG - Chr1:53764908-53764927 MsG0180002956.01.T01:CDS 40.0%
! CCACCATTTGATTCGTTTCT+TGG + Chr1:53763898-53763917 None:intergenic 40.0%
! GAGAATGCAAGAGCACAATA+AGG + Chr1:53764706-53764725 None:intergenic 40.0%
! TGAAGGATGCTTGTTAGAAG+AGG - Chr1:53764902-53764921 MsG0180002956.01.T01:CDS 40.0%
! TTGGCCAGAGATTTTTGCAA+AGG - Chr1:53764863-53764882 MsG0180002956.01.T01:CDS 40.0%
!! ACGAACTACTTTTGAGCTAG+TGG + Chr1:53763276-53763295 None:intergenic 40.0%
!! AGATGATGGTGAAAGCATTG+CGG - Chr1:53764812-53764831 MsG0180002956.01.T01:CDS 40.0%
!! TGATGATGGTGAATGCATTG+TGG - Chr1:53764782-53764801 MsG0180002956.01.T01:CDS 40.0%
!!! ACTTTTTCACACCAAGGAGT+TGG + Chr1:53763367-53763386 None:intergenic 40.0%
ATTGGAATTGGTGCATGGGA+AGG - Chr1:53763299-53763318 MsG0180002956.01.T01:intron 45.0%
! ATGATGATGCTGGTACGTCT+TGG - Chr1:53764844-53764863 MsG0180002956.01.T01:CDS 45.0%
GCAAGATCCATGCACCATCA+TGG + Chr1:53763418-53763437 None:intergenic 50.0%
GGCTTCTTCGGGTCTAAGAA+TGG + Chr1:53764007-53764026 None:intergenic 50.0%
TGGGAAGGTGAAGAAGACAC+TGG - Chr1:53763314-53763333 MsG0180002956.01.T01:intron 50.0%
TTCTGCACGTCCAGGAACAA+TGG + Chr1:53763631-53763650 None:intergenic 50.0%
! GACGAAGACGATGATGATGC+TGG - Chr1:53764834-53764853 MsG0180002956.01.T01:CDS 50.0%
! GCACTTACCATGATGGTGCA+TGG - Chr1:53763408-53763427 MsG0180002956.01.T01:intron 50.0%
ATGGTGGTTTCTGCACGTCC+AGG + Chr1:53763639-53763658 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 53763029 53764992 53763029 ID=MsG0180002956.01;Name=MsG0180002956.01
Chr1 mRNA 53763029 53764992 53763029 ID=MsG0180002956.01.T01;Parent=MsG0180002956.01;Name=MsG0180002956.01.T01;_AED=0.11;_eAED=0.11;_QI=0|0|0|0.75|1|1|4|0|331
Chr1 exon 53764543 53764992 53764543 ID=MsG0180002956.01.T01:exon:47787;Parent=MsG0180002956.01.T01
Chr1 exon 53764359 53764457 53764359 ID=MsG0180002956.01.T01:exon:47786;Parent=MsG0180002956.01.T01
Chr1 exon 53763986 53764174 53763986 ID=MsG0180002956.01.T01:exon:47785;Parent=MsG0180002956.01.T01
Chr1 exon 53763029 53763286 53763029 ID=MsG0180002956.01.T01:exon:47784;Parent=MsG0180002956.01.T01
Chr1 CDS 53764543 53764992 53764543 ID=MsG0180002956.01.T01:cds;Parent=MsG0180002956.01.T01
Chr1 CDS 53764359 53764457 53764359 ID=MsG0180002956.01.T01:cds;Parent=MsG0180002956.01.T01
Chr1 CDS 53763986 53764174 53763986 ID=MsG0180002956.01.T01:cds;Parent=MsG0180002956.01.T01
Chr1 CDS 53763029 53763286 53763029 ID=MsG0180002956.01.T01:cds;Parent=MsG0180002956.01.T01
Gene Sequence

>MsG0180002956.01.T01

ATGGCTCTCAATTCTTCTTCAAGTAAGGATTTCAAATTCACACTCAGAGATGATGAACTTCTACGTTTCCTCTACAACAAAATCCATAACAAATCTCTGCCAAATTATATCGCCATTCTTGAATACGATTTGTTTGGTACCCTAAAGAATCCTTGGGATATTTGGGAAGAGTTTGGAGCTTCTCGCTCATATTGTGAAAAAGACCTATATTTCTTCACTACCTTAACCTTAGAGAAGAAATCTACCACTAGCTCAAAAGTAGTTCGTTCTATTGGAATTGGTGCATGGGAAGGTGAAGAAGACACTGGAAAAATTATAGTTGCAAAAAACAAGAACCAACTCCTTGGTGTGAAAAAGTGTTTTCGATTTGAGAATAGTGGCACTTACCATGATGGTGCATGGATCTTGCATGAATATAGCCTTGACAAATCTTTGATAAGAAATCCTTTGGCCAAAAATTATGTTCTATGCAGATTTAGAAAGAATATTGATGCACAAGGTCAAGAAAGAAAGACCATTGTTCCTGGACGTGCAGAAACCACCATATCTGGAAATAAAGATAAAGATTCGTTAACGAAATATATAATTTATAAAGAACCAAGAAACGAATCAAATGGTGGACACAATAGCACAAATATGGTTAATACAAAGTCTCATTATAAGGAAGATGAAAAAGATGTGAGAAATATTTGGCCAAGAGAGACAACCATTCTTAGACCCGAAGAAGCCAACAAATTTGAGAATATTAACCAAAATCCAATAGTTCAACCTGACAATGAAGATGATGATGATGGTGAATGCATTGTGGACAAAGAAGATGATGGTGAAAGCATTGCGGACGAAGACGATGATGATGCTGGTACGTCTTGGCCAGAGATTTTTGCAAAGGAGCTGATAGAGATGAATGAAGGATGCTTGTTAGAAGAGGAAGAGGTTCAAATGATTCCCAATAAATTTCATGAGGAGTTATTATTGGATAATACTAACATAAAATAA
Protein sequence

>MsG0180002956.01.T01

MALNSSSSKDFKFTLRDDELLRFLYNKIHNKSLPNYIAILEYDLFGTLKNPWDIWEEFGASRSYCEKDLYFFTTLTLEKKSTTSSKVVRSIGIGAWEGEEDTGKIIVAKNKNQLLGVKKCFRFENSGTYHDGAWILHEYSLDKSLIRNPLAKNYVLCRFRKNIDAQGQERKTIVPGRAETTISGNKDKDSLTKYIIYKEPRNESNGGHNSTNMVNTKSHYKEDEKDVRNIWPRETTILRPEEANKFENINQNPIVQPDNEDDDDGECIVDKEDDGESIADEDDDDAGTSWPEIFAKELIEMNEGCLLEEEEVQMIPNKFHEELLLDNTNIK*