AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002970.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002970.01.T01 MTR_1g053835 65.138 327 39 11 1 270 80 388 3.91e-125 363
MsG0180002970.01.T01 MTR_7g093030 47.633 338 82 14 1 270 33 343 1.13e-73 230
MsG0180002970.01.T01 MTR_7g093030 45.349 258 59 12 1 190 33 276 6.15e-47 159
MsG0180002970.01.T01 MTR_7g098250 51.240 121 47 2 152 272 288 396 5.58e-32 123
MsG0180002970.01.T01 MTR_2g027860 52.459 122 48 2 152 273 75 186 1.66e-31 119
MsG0180002970.01.T01 MTR_7g088070 50.847 118 49 1 152 269 76 184 1.35e-30 115
MsG0180002970.01.T01 MTR_7g088070 51.695 118 44 3 152 269 76 180 5.37e-30 114
MsG0180002970.01.T01 MTR_1g080330 52.941 119 44 3 152 270 201 307 2.45e-29 114
MsG0180002970.01.T01 MTR_7g115530 49.167 120 47 3 151 269 243 349 7.19e-28 111
MsG0180002970.01.T01 MTR_2g086450 43.411 129 63 3 151 270 70 197 6.87e-23 97.1
MsG0180002970.01.T01 MTR_6g032990 44.444 126 62 2 151 270 67 190 1.88e-22 95.9
MsG0180002970.01.T01 MTR_5g027440 42.400 125 62 2 151 270 50 169 4.59e-22 93.2
MsG0180002970.01.T01 MTR_6g444980 40.833 120 62 3 151 270 60 170 2.43e-19 87.0
MsG0180002970.01.T01 MTR_4g081710 41.463 123 65 4 151 270 42 160 8.77e-19 85.1
MsG0180002970.01.T01 MTR_6g444980 43.243 111 54 3 151 261 60 161 1.05e-18 84.7
MsG0180002970.01.T01 MTR_1g053800 43.802 121 55 2 151 271 202 309 3.68e-18 83.2
MsG0180002970.01.T01 MTR_1g080330 47.368 95 38 3 151 245 200 282 8.11e-18 81.6
MsG0180002970.01.T01 MTR_5g041350 36.923 130 62 4 151 270 40 159 9.13e-18 82.4
MsG0180002970.01.T01 MTR_7g115530 44.330 97 40 3 151 246 243 326 1.54e-16 78.6
MsG0180002970.01.T01 MTR_7g068600 37.267 161 73 7 126 273 6 151 1.17e-15 74.7
MsG0180002970.01.T01 MTR_2g027800 38.053 113 56 2 151 263 64 162 5.22e-15 73.9
MsG0180002970.01.T01 MTR_2g027800 36.364 121 61 2 151 263 64 176 1.79e-14 72.4
MsG0180002970.01.T01 MTR_1g090670 35.294 119 64 2 150 263 68 178 6.10e-14 70.9
MsG0180002970.01.T01 MTR_1g053830 75.000 40 10 0 151 190 43 82 3.86e-13 64.3
MsG0180002970.01.T01 MTR_7g093010 38.095 126 53 3 151 270 238 344 1.51e-11 64.3
MsG0180002970.01.T01 MTR_7g115530 65.000 40 14 0 151 190 243 282 3.13e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002970.01.T01 AT5G06800 67.227 119 26 2 151 269 217 322 2.98e-43 151
MsG0180002970.01.T01 AT5G06800 67.227 119 31 2 151 269 217 327 1.40e-42 150
MsG0180002970.01.T01 AT2G01060 50.000 126 44 2 152 273 41 151 7.86e-33 122
MsG0180002970.01.T01 AT4G28610 43.478 161 70 4 117 267 206 355 8.33e-31 119
MsG0180002970.01.T01 AT5G29000 50.000 118 50 3 152 269 214 322 1.20e-29 115
MsG0180002970.01.T01 AT5G29000 50.000 118 50 3 152 269 214 322 1.20e-29 115
MsG0180002970.01.T01 AT5G29000 50.000 118 50 3 152 269 257 365 1.61e-29 115
MsG0180002970.01.T01 AT3G13040 48.760 121 53 2 151 270 266 378 9.35e-29 114
MsG0180002970.01.T01 AT3G13040 48.760 121 53 2 151 270 266 378 9.35e-29 114
MsG0180002970.01.T01 AT3G13040 48.760 121 53 2 151 270 266 378 9.35e-29 114
MsG0180002970.01.T01 AT2G01060 48.718 117 41 2 161 273 1 102 6.24e-28 107
MsG0180002970.01.T01 AT3G04450 49.593 123 43 2 152 269 224 332 7.79e-28 110
MsG0180002970.01.T01 AT3G04450 49.593 123 43 2 152 269 224 332 7.79e-28 110
MsG0180002970.01.T01 AT3G04450 49.593 123 43 2 152 269 264 372 9.76e-28 111
MsG0180002970.01.T01 AT2G20400 47.863 117 49 2 151 267 256 360 2.56e-25 103
MsG0180002970.01.T01 AT2G20400 47.899 119 47 3 151 267 256 361 4.72e-25 103
MsG0180002970.01.T01 AT5G18240 44.615 130 55 3 151 270 70 192 2.05e-23 98.6
MsG0180002970.01.T01 AT5G18240 44.615 130 55 3 151 270 70 192 2.05e-23 98.6
MsG0180002970.01.T01 AT3G04030 43.307 127 61 2 151 270 70 192 8.84e-23 97.1
MsG0180002970.01.T01 AT3G24120 41.732 127 67 1 151 270 66 192 1.19e-22 95.1
MsG0180002970.01.T01 AT1G79430 43.333 120 59 3 151 270 59 169 1.47e-22 95.9
MsG0180002970.01.T01 AT3G04030 42.188 128 63 2 150 270 68 191 1.99e-22 95.9
MsG0180002970.01.T01 AT1G69580 42.969 128 62 4 151 270 59 183 6.27e-22 94.0
MsG0180002970.01.T01 AT4G13640 42.017 119 69 0 151 269 62 180 8.96e-22 92.8
MsG0180002970.01.T01 AT1G69580 42.969 128 63 4 151 270 59 184 1.15e-21 93.2
MsG0180002970.01.T01 AT3G24120 40.769 130 67 2 151 270 66 195 1.18e-20 89.7
MsG0180002970.01.T01 AT5G18240 43.846 130 54 4 151 270 70 190 1.53e-20 90.5
MsG0180002970.01.T01 AT4G13640 40.984 122 69 1 151 269 62 183 7.38e-20 87.4
MsG0180002970.01.T01 AT3G04030 41.667 120 59 2 158 270 1 116 3.16e-19 85.9
MsG0180002970.01.T01 AT1G79430 41.593 113 57 3 158 270 1 104 7.00e-19 84.7
MsG0180002970.01.T01 AT5G18240 41.538 130 53 4 151 270 70 186 3.64e-18 84.0
MsG0180002970.01.T01 AT5G18240 41.538 130 53 4 151 270 70 186 3.64e-18 84.0
MsG0180002970.01.T01 AT3G04030 40.157 127 59 3 151 270 70 186 1.44e-17 82.0
MsG0180002970.01.T01 AT5G06800 75.000 48 12 0 151 198 217 264 3.20e-17 79.7
MsG0180002970.01.T01 AT3G12730 35.537 121 72 2 151 270 48 163 5.22e-17 78.6
MsG0180002970.01.T01 AT5G45580 35.294 136 72 3 151 275 32 162 8.54e-17 78.6
MsG0180002970.01.T01 AT5G45580 35.294 136 72 3 151 275 48 178 1.39e-16 78.2
MsG0180002970.01.T01 AT5G18240 39.837 123 51 4 158 270 1 110 1.05e-14 73.2
MsG0180002970.01.T01 AT3G04030 38.333 120 57 3 158 270 1 110 4.82e-14 71.2
MsG0180002970.01.T01 AT3G12730 33.333 114 70 2 158 270 1 109 1.25e-13 68.2
MsG0180002970.01.T01 AT5G29000 60.870 46 16 1 152 195 257 302 5.55e-11 62.4

Find 38 sgRNAs with CRISPR-Local

Find 135 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAAATTTGATTGTGTTCT+TGG 0.148860 1:-54041328 MsG0180002970.01.T01:CDS
TTCTAATAAGGTTAATGATT+TGG 0.230770 1:-54042204 MsG0180002970.01.T01:CDS
TTTCCATTAGAATGAATTAA+TGG 0.240080 1:+54041963 None:intergenic
CTATACTTGTCTTCTGTTGT+TGG 0.292235 1:+54039228 None:intergenic
AGTCCATTAATTCATTCTAA+TGG 0.307810 1:-54041966 MsG0180002970.01.T01:CDS
CAACACCGAAAGCGATATTA+AGG 0.350678 1:-54041288 MsG0180002970.01.T01:CDS
TCTCTACATGAGTTCTTTCT+CGG 0.372264 1:+54040287 None:intergenic
ATCAGCCTTAATATCGCTTT+CGG 0.385906 1:+54041283 None:intergenic
GAAAATTGAGATATCAAAAG+AGG 0.399023 1:+54042227 None:intergenic
AAATTACCTTGAGCTTGTTC+TGG 0.414813 1:+54041190 None:intergenic
ATTGATTTGTTGTTGAATCA+AGG 0.420361 1:+54042150 None:intergenic
CCACAATATGATTACCAAGT+TGG 0.422118 1:-54042266 MsG0180002970.01.T01:CDS
GGAATTCCCTTTGTATCAAA+GGG 0.429673 1:-54042183 MsG0180002970.01.T01:CDS
TACTACTTTCATTTCCAACT+TGG 0.455279 1:+54042252 None:intergenic
TGGAATTCCCTTTGTATCAA+AGG 0.460488 1:-54042184 MsG0180002970.01.T01:CDS
AAAATTACAATTGCGAATAG+AGG 0.460707 1:-54039282 MsG0180002970.01.T01:CDS
AATGTTGTTAGAAAGTAAAA+CGG 0.467944 1:-54041354 MsG0180002970.01.T01:intron
GAAAGAACTCATGTAGAGAA+TGG 0.468797 1:-54040283 MsG0180002970.01.T01:CDS
CTGAAGTTGGTGACTCAAAA+CGG 0.487919 1:+54042321 None:intergenic
CTCCAAGAAAATTCCCACAT+TGG 0.501031 1:+54042051 None:intergenic
CCAACTTGGTAATCATATTG+TGG 0.502891 1:+54042266 None:intergenic
TTATGCAACTGAAATTTGCA+TGG 0.523528 1:-54042294 MsG0180002970.01.T01:CDS
AAATAATATAGCATTACGAT+TGG 0.548224 1:+54040250 None:intergenic
ACAACAGAAGACAAGTATAG+TGG 0.550887 1:-54039225 MsG0180002970.01.T01:CDS
TCTTGGCTGCTTGATTGTAG+AGG 0.553991 1:-54041311 MsG0180002970.01.T01:CDS
TATGCAACTGAAATTTGCAT+GGG 0.562190 1:-54042293 MsG0180002970.01.T01:CDS
AATTTGACAACTCAAAATTG+TGG 0.568956 1:+54042123 None:intergenic
AAAATTGAGATATCAAAAGA+GGG 0.579511 1:+54042228 None:intergenic
CAGTGGCTTGCAAATTAGAG+AGG 0.586499 1:-54040142 MsG0180002970.01.T01:CDS
AGCTGCAGCTAGACGTTCAG+AGG 0.587380 1:-54040113 MsG0180002970.01.T01:CDS
GAAAGCGATATTAAGGCTGA+TGG 0.589838 1:-54041281 MsG0180002970.01.T01:CDS
TTAAGGCTGATGGATTCAGA+TGG 0.601499 1:-54041271 MsG0180002970.01.T01:CDS
AATCCAATAAAATCCAATGT+GGG 0.615499 1:-54042064 MsG0180002970.01.T01:CDS
AAATCCAATAAAATCCAATG+TGG 0.616777 1:-54042065 MsG0180002970.01.T01:CDS
GCGTCTTCATGAACAACTAG+AGG 0.636358 1:-54040091 MsG0180002970.01.T01:intron
TATATGCCAGAACAAGCTCA+AGG 0.643937 1:-54041196 MsG0180002970.01.T01:CDS
TAATGGACTCATACTAACTG+AGG 0.670460 1:+54041980 None:intergenic
CAATTGCGAATAGAGGAACA+AGG 0.704087 1:-54039275 MsG0180002970.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTAAATGATAAATAG+AGG - Chr1:54041085-54041104 MsG0180002970.01.T01:intron 10.0%
!!! TATTTTTTCTATTTCATTAT+TGG - Chr1:54039657-54039676 MsG0180002970.01.T01:intron 10.0%
!! TAAATTCAATATAGATGATT+TGG + Chr1:54041861-54041880 None:intergenic 15.0%
!! TCTAAGAAAATAAAAAACTT+CGG - Chr1:54042001-54042020 MsG0180002970.01.T01:CDS 15.0%
!!! AATTTTCTTACATTAATTTC+TGG - Chr1:54042224-54042243 MsG0180002970.01.T01:CDS 15.0%
!!! TTCTTGATTGTTTATTTTAT+CGG + Chr1:54039984-54040003 None:intergenic 15.0%
!! AAAATTGAGATATCAAAAGA+GGG + Chr1:54039318-54039337 None:intergenic 20.0%
!! AAATAATATAGCATTACGAT+TGG + Chr1:54041296-54041315 None:intergenic 20.0%
!! AATGTTGTTAGAAAGTAAAA+CGG - Chr1:54040189-54040208 MsG0180002970.01.T01:intron 20.0%
!! ATGAACTTAAATTTCATAGA+TGG + Chr1:54041728-54041747 None:intergenic 20.0%
!! CTAATAAGAAAAAAAATCGA+AGG - Chr1:54041970-54041989 MsG0180002970.01.T01:CDS 20.0%
!! GTATTATAATAACAATCACT+CGG - Chr1:54041910-54041929 MsG0180002970.01.T01:intron 20.0%
!! TATCTATAATGTAAAGTACA+TGG - Chr1:54040418-54040437 MsG0180002970.01.T01:intron 20.0%
!! TCGATAAGATAAAATAAACT+AGG - Chr1:54040805-54040824 MsG0180002970.01.T01:intron 20.0%
!! TGTCACAAAATAATGTATAA+AGG + Chr1:54041493-54041512 None:intergenic 20.0%
!! TTCTAATAAGGTTAATGATT+TGG - Chr1:54039339-54039358 MsG0180002970.01.T01:intron 20.0%
!! TTTCCATTAGAATGAATTAA+TGG + Chr1:54039583-54039602 None:intergenic 20.0%
!!! AGTTTTTTATTTTCTTAGAC+TGG + Chr1:54042000-54042019 None:intergenic 20.0%
!!! ATCTCAATTTTCTTCTAATA+AGG - Chr1:54039327-54039346 MsG0180002970.01.T01:intron 20.0%
!!! TCAATTTTCTAACATATGAA+AGG + Chr1:54041066-54041085 None:intergenic 20.0%
! AAAAAGCTCAAAACAACTTA+TGG - Chr1:54040903-54040922 MsG0180002970.01.T01:intron 25.0%
! AAAATTACAATTGCGAATAG+AGG - Chr1:54042261-54042280 MsG0180002970.01.T01:CDS 25.0%
! AAAGAGAAGATGAAAGATAA+CGG + Chr1:54040121-54040140 None:intergenic 25.0%
! AAATCCAATAAAATCCAATG+TGG - Chr1:54039478-54039497 MsG0180002970.01.T01:intron 25.0%
! AAGAAACATATCCGATATTA+TGG - Chr1:54042046-54042065 MsG0180002970.01.T01:CDS 25.0%
! AATCCAATAAAATCCAATGT+GGG - Chr1:54039479-54039498 MsG0180002970.01.T01:intron 25.0%
! AATTTGACAACTCAAAATTG+TGG + Chr1:54039423-54039442 None:intergenic 25.0%
! ACTGAAATTCTCCATAATAT+CGG + Chr1:54042060-54042079 None:intergenic 25.0%
! AGAAAATACAAATACTAAGC+AGG + Chr1:54042186-54042205 None:intergenic 25.0%
! AGGAGTTTAAAAAATGTCTA+TGG + Chr1:54042166-54042185 None:intergenic 25.0%
! AGTCCATTAATTCATTCTAA+TGG - Chr1:54039577-54039596 MsG0180002970.01.T01:intron 25.0%
! ATATCGGATATGTTTCTTTA+AGG + Chr1:54042044-54042063 None:intergenic 25.0%
! ATTATGAGTACGTTGAAATA+CGG + Chr1:54041650-54041669 None:intergenic 25.0%
! GAAAATTGAGATATCAAAAG+AGG + Chr1:54039319-54039338 None:intergenic 25.0%
! TATCGTAATATCAAGTTTCA+TGG + Chr1:54041579-54041598 None:intergenic 25.0%
! TGTGGTTATAAACTAATCTA+AGG - Chr1:54040514-54040533 MsG0180002970.01.T01:intron 25.0%
! TTAAAAAATGTCTATGGATG+CGG + Chr1:54042160-54042179 None:intergenic 25.0%
!! CGTAATGCTATATTATTTTC+CGG - Chr1:54041298-54041317 MsG0180002970.01.T01:CDS 25.0%
!! CTTCTTTATTACAACTTTTC+AGG + Chr1:54041029-54041048 None:intergenic 25.0%
!! TGGATTTTATTGGATTTTTC+AGG + Chr1:54039475-54039494 None:intergenic 25.0%
!!! ATTGATTTGTTGTTGAATCA+AGG + Chr1:54039396-54039415 None:intergenic 25.0%
AAAACAATGGAGAGAGAAAA+AGG - Chr1:54040699-54040718 MsG0180002970.01.T01:intron 30.0%
ACATGGTGTATGAATTAAAC+AGG - Chr1:54040435-54040454 MsG0180002970.01.T01:intron 30.0%
ACTTCTATTGGTCAAAACAA+TGG - Chr1:54040686-54040705 MsG0180002970.01.T01:intron 30.0%
CAAGTAAAACACGAATAAGA+TGG - Chr1:54039851-54039870 MsG0180002970.01.T01:intron 30.0%
CGAAAATTTGATTGTGTTCT+TGG - Chr1:54040215-54040234 MsG0180002970.01.T01:intron 30.0%
GTCAAAAAGTTGTCAAAAAG+TGG - Chr1:54040764-54040783 MsG0180002970.01.T01:intron 30.0%
TAATATCAAGTTTCATGGAG+CGG + Chr1:54041574-54041593 None:intergenic 30.0%
TACTACTTTCATTTCCAACT+TGG + Chr1:54039294-54039313 None:intergenic 30.0%
TATGCAACTGAAATTTGCAT+GGG - Chr1:54039250-54039269 MsG0180002970.01.T01:CDS 30.0%
TCAACAAAGTTGTCATCAAA+TGG + Chr1:54040651-54040670 None:intergenic 30.0%
TTAGTCTAATCTCACTTCAA+TGG - Chr1:54041149-54041168 MsG0180002970.01.T01:intron 30.0%
TTATGCAACTGAAATTTGCA+TGG - Chr1:54039249-54039268 MsG0180002970.01.T01:CDS 30.0%
! ATTCCCACATTGGATTTTAT+TGG + Chr1:54039485-54039504 None:intergenic 30.0%
! GATTTTCCCTTTGATACAAA+GGG + Chr1:54039369-54039388 None:intergenic 30.0%
! GTTTCTTTTCATAATTGCAG+TGG - Chr1:54041384-54041403 MsG0180002970.01.T01:intron 30.0%
! TCTGCAAATGACTTTTAACA+TGG + Chr1:54040310-54040329 None:intergenic 30.0%
! TGATTTTCCCTTTGATACAA+AGG + Chr1:54039370-54039389 None:intergenic 30.0%
! TTATCGAGGTGTTATAATCA+GGG - Chr1:54041673-54041692 MsG0180002970.01.T01:intron 30.0%
!! GTACTCATAATTTGTTATCG+AGG - Chr1:54041659-54041678 MsG0180002970.01.T01:intron 30.0%
!! TTTTCAGGAGATCTAAGAAT+TGG + Chr1:54039460-54039479 None:intergenic 30.0%
!!! TCTTGTTTTCTCACTTCTAT+TGG - Chr1:54040674-54040693 MsG0180002970.01.T01:intron 30.0%
!!! TTTTTTATGTTTAGCGACAG+CGG + Chr1:54042117-54042136 None:intergenic 30.0%
AACAAACTGCTAACTGTTTC+AGG - Chr1:54041228-54041247 MsG0180002970.01.T01:CDS 35.0%
AACAGTTTCATAAGTGCTTG+TGG - Chr1:54040959-54040978 MsG0180002970.01.T01:intron 35.0%
ACAAACTGCTAACTGTTTCA+GGG - Chr1:54041229-54041248 MsG0180002970.01.T01:CDS 35.0%
ACAACAGAAGACAAGTATAG+TGG - Chr1:54042318-54042337 MsG0180002970.01.T01:CDS 35.0%
ACAAGCACTTATGAAACTGT+TGG + Chr1:54040960-54040979 None:intergenic 35.0%
ACATTTCCTGTTGACATAGT+TGG + Chr1:54039788-54039807 None:intergenic 35.0%
ATCAGCCTTAATATCGCTTT+CGG + Chr1:54040263-54040282 None:intergenic 35.0%
CCACAATATGATTACCAAGT+TGG - Chr1:54039277-54039296 MsG0180002970.01.T01:CDS 35.0%
CCATGTTTGGATTTGATTAG+AGG - Chr1:54041702-54041721 MsG0180002970.01.T01:intron 35.0%
CCTCTAATCAAATCCAAACA+TGG + Chr1:54041705-54041724 None:intergenic 35.0%
CTATACTTGTCTTCTGTTGT+TGG + Chr1:54042318-54042337 None:intergenic 35.0%
CTTATGAAACTGTTGGAGAT+TGG + Chr1:54040953-54040972 None:intergenic 35.0%
GAAAGAACTCATGTAGAGAA+TGG - Chr1:54041260-54041279 MsG0180002970.01.T01:CDS 35.0%
GGAATTCCCTTTGTATCAAA+GGG - Chr1:54039360-54039379 MsG0180002970.01.T01:intron 35.0%
GTTGCATAAAAAGCTGAAGT+TGG + Chr1:54039238-54039257 None:intergenic 35.0%
TCTCTACATGAGTTCTTTCT+CGG + Chr1:54041259-54041278 None:intergenic 35.0%
TGAAGTGAGATTAGACTAAC+TGG + Chr1:54041148-54041167 None:intergenic 35.0%
TGGAATTCCCTTTGTATCAA+AGG - Chr1:54039359-54039378 MsG0180002970.01.T01:intron 35.0%
! AAACTGTTGGAGATTGGTTA+TGG + Chr1:54040947-54040966 None:intergenic 35.0%
! AAATTACCTTGAGCTTGTTC+TGG + Chr1:54040356-54040375 None:intergenic 35.0%
! ATCCAATGTGGGAATTTTCT+TGG - Chr1:54039490-54039509 MsG0180002970.01.T01:intron 35.0%
! CCAACTTGGTAATCATATTG+TGG + Chr1:54039280-54039299 None:intergenic 35.0%
! GTTATCGAGGTGTTATAATC+AGG - Chr1:54041672-54041691 MsG0180002970.01.T01:intron 35.0%
! TCGAATCTTTCGTGTTTTGT+AGG - Chr1:54039695-54039714 MsG0180002970.01.T01:intron 35.0%
! TGTGGTTCATATGATTTTCC+AGG - Chr1:54039723-54039742 MsG0180002970.01.T01:intron 35.0%
!! AAACTAATCTAAGGCACTGT+TGG - Chr1:54040523-54040542 MsG0180002970.01.T01:intron 35.0%
!! TAAATGCATCGGTTTACTTC+TGG - Chr1:54039937-54039956 MsG0180002970.01.T01:intron 35.0%
!! TAATGGACTCATACTAACTG+AGG + Chr1:54039566-54039585 None:intergenic 35.0%
AAGAAATTCAGCTGTGAACC+TGG + Chr1:54039744-54039763 None:intergenic 40.0%
ACATAGAGCTTAGAACACAG+GGG - Chr1:54040490-54040509 MsG0180002970.01.T01:intron 40.0%
AGAGAGAAAAAGGAAGAGAG+AGG - Chr1:54040709-54040728 MsG0180002970.01.T01:intron 40.0%
ATCAGGGACATATCCATGTT+TGG - Chr1:54041689-54041708 MsG0180002970.01.T01:intron 40.0%
CAACACCGAAAGCGATATTA+AGG - Chr1:54040255-54040274 MsG0180002970.01.T01:CDS 40.0%
CAATTGCGAATAGAGGAACA+AGG - Chr1:54042268-54042287 MsG0180002970.01.T01:CDS 40.0%
CCGAGAGTAAGTAAATGCAT+CGG - Chr1:54039926-54039945 MsG0180002970.01.T01:intron 40.0%
CCGATGCATTTACTTACTCT+CGG + Chr1:54039929-54039948 None:intergenic 40.0%
CTCCAAGAAAATTCCCACAT+TGG + Chr1:54039495-54039514 None:intergenic 40.0%
GAAAGCGATATTAAGGCTGA+TGG - Chr1:54040262-54040281 MsG0180002970.01.T01:CDS 40.0%
GAATTATCCACATCACACTC+CGG + Chr1:54041320-54041339 None:intergenic 40.0%
GACATAGAGCTTAGAACACA+GGG - Chr1:54040489-54040508 MsG0180002970.01.T01:intron 40.0%
GAGTGTGATGTGGATAATTC+CGG - Chr1:54041320-54041339 MsG0180002970.01.T01:CDS 40.0%
GCTCAAAACAACTTATGGAC+AGG - Chr1:54040908-54040927 MsG0180002970.01.T01:intron 40.0%
GGAAATGTCTCAACGAATTC+CGG - Chr1:54039800-54039819 MsG0180002970.01.T01:intron 40.0%
TAAGCGAGAAATGATTGCAC+CGG + Chr1:54041342-54041361 None:intergenic 40.0%
TATATGCCAGAACAAGCTCA+AGG - Chr1:54040347-54040366 MsG0180002970.01.T01:intron 40.0%
TCAGTCAAATGTGACTGATG+TGG - Chr1:54042075-54042094 MsG0180002970.01.T01:CDS 40.0%
TGACATAGAGCTTAGAACAC+AGG - Chr1:54040488-54040507 MsG0180002970.01.T01:intron 40.0%
! CGTGTTTTGTAGGTTTCTTG+TGG - Chr1:54039705-54039724 MsG0180002970.01.T01:intron 40.0%
! TTATTTTCCGGAGTGTGATG+TGG - Chr1:54041310-54041329 MsG0180002970.01.T01:CDS 40.0%
!! CTGAAGTTGGTGACTCAAAA+CGG + Chr1:54039225-54039244 None:intergenic 40.0%
!! TTAAGGCTGATGGATTCAGA+TGG - Chr1:54040272-54040291 MsG0180002970.01.T01:CDS 40.0%
AAATGTCTATGGATGCGGTC+TGG + Chr1:54042155-54042174 None:intergenic 45.0%
AACCGATGATCCATACAAGC+AGG + Chr1:54039830-54039849 None:intergenic 45.0%
ACCCTGCTTGTATGGATCAT+CGG - Chr1:54039825-54039844 MsG0180002970.01.T01:intron 45.0%
ACCGATGATCCATACAAGCA+GGG + Chr1:54039829-54039848 None:intergenic 45.0%
AGACTACACTTCGCAACTAG+AGG + Chr1:54041947-54041966 None:intergenic 45.0%
ATCCATACAAGCAGGGTTTC+CGG + Chr1:54039822-54039841 None:intergenic 45.0%
CTGTCTCCAACTATGTCAAC+AGG - Chr1:54039779-54039798 MsG0180002970.01.T01:intron 45.0%
GCGTCTTCATGAACAACTAG+AGG - Chr1:54041452-54041471 MsG0180002970.01.T01:intron 45.0%
TCTTGGCTGCTTGATTGTAG+AGG - Chr1:54040232-54040251 MsG0180002970.01.T01:intron 45.0%
TGACTGATGTGGCCTCAATA+TGG - Chr1:54042086-54042105 MsG0180002970.01.T01:CDS 45.0%
! CAGTGGCTTGCAAATTAGAG+AGG - Chr1:54041401-54041420 MsG0180002970.01.T01:intron 45.0%
!! ATAAAAGTAATTAAATTATA+AGG + Chr1:54039640-54039659 None:intergenic 5.0%
AGCTTAGAACACAGGGGATG+TGG - Chr1:54040496-54040515 MsG0180002970.01.T01:intron 50.0%
GTTGAGTGTGTGAATCGCCT+TGG - Chr1:54039898-54039917 MsG0180002970.01.T01:intron 50.0%
TTCCGGAAACCCTGCTTGTA+TGG - Chr1:54039817-54039836 MsG0180002970.01.T01:intron 50.0%
ACTTACTCTCGGCACCACCA+AGG + Chr1:54039918-54039937 None:intergenic 55.0%
AGCTGCAGCTAGACGTTCAG+AGG - Chr1:54041430-54041449 MsG0180002970.01.T01:intron 55.0%
GAGTGTGTGAATCGCCTTGG+TGG - Chr1:54039901-54039920 MsG0180002970.01.T01:intron 55.0%
TATGTTTAGCGACAGCGGCG+TGG + Chr1:54042112-54042131 None:intergenic 55.0%
ACAGCGGCGTGGCCATATTG+AGG + Chr1:54042101-54042120 None:intergenic 60.0%
CGGTCTGGACTGCAACAGCG+AGG + Chr1:54042140-54042159 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 54039217 54042348 54039217 ID=MsG0180002970.01;Name=MsG0180002970.01
Chr1 mRNA 54039217 54042348 54039217 ID=MsG0180002970.01.T01;Parent=MsG0180002970.01;Name=MsG0180002970.01.T01;_AED=0.31;_eAED=0.32;_QI=0|0|0|1|0.5|0.8|5|0|275
Chr1 exon 54041950 54042348 54041950 ID=MsG0180002970.01.T01:exon:47845;Parent=MsG0180002970.01.T01
Chr1 exon 54041177 54041365 54041177 ID=MsG0180002970.01.T01:exon:47844;Parent=MsG0180002970.01.T01
Chr1 exon 54040242 54040315 54040242 ID=MsG0180002970.01.T01:exon:47843;Parent=MsG0180002970.01.T01
Chr1 exon 54040092 54040161 54040092 ID=MsG0180002970.01.T01:exon:47842;Parent=MsG0180002970.01.T01
Chr1 exon 54039217 54039312 54039217 ID=MsG0180002970.01.T01:exon:47841;Parent=MsG0180002970.01.T01
Chr1 CDS 54041950 54042348 54041950 ID=MsG0180002970.01.T01:cds;Parent=MsG0180002970.01.T01
Chr1 CDS 54041177 54041365 54041177 ID=MsG0180002970.01.T01:cds;Parent=MsG0180002970.01.T01
Chr1 CDS 54040242 54040315 54040242 ID=MsG0180002970.01.T01:cds;Parent=MsG0180002970.01.T01
Chr1 CDS 54040092 54040161 54040092 ID=MsG0180002970.01.T01:cds;Parent=MsG0180002970.01.T01
Chr1 CDS 54039217 54039312 54039217 ID=MsG0180002970.01.T01:cds;Parent=MsG0180002970.01.T01
Gene Sequence

>MsG0180002970.01.T01

ATGAGCCGTTTTGAGTCACCAACTTCAGCTTTTTATGCAACTGAAATTTGCATGGGTTTTCCACAATATGATTACCAAGTTGGAAATGAAAGTAGTAACCCTCTTTTGATATCTCAATTTTCTTCTAATAAGGTTAATGATTTGGAATTCCCTTTGTATCAAAGGGAAAATCATTACCTTGATTCAACAACAAATCAATCTAGCCACAATTTTGAGTTGTCAAATTTAAACACTTTGCAACCAATTCTTAGATCTCCTGAAAAATCCAATAAAATCCAATGTGGGAATTTTCTTGGAGAAAATTTTCTCTCAGTTGAGCAAAATAAATTCTTCATTGATGATGCTGCCTCAGTTAGTATGAGTCCATTAATTCATTCTAATGGAAATCAAGATCATAAAAAAGTAAAACGGATGCGAAAATTTGATTGTGTTCTTGGCTGCTTGATTGTAGAGGCAACACCGAAAGCGATATTAAGGCTGATGGATTCAGATGGATTGACAATTTTCCATGTTAAAAGTCATTTGCAGAAGTATAGAATTGCAAAATATATGCCAGAACAAGCTCAAGGTAATTTGTTATCATACTCAGGAAATCCGAGAAAGAACTCATGTAGAGAATGGAATCTTGATGCCAATCGTAATGCTATATTATTTTCCGGAGTTGGCTTGCAAATTAGAGAGGCACTTCAGCTGCAGCTAGACGTTCAGAGGCGTCTTCATGAACAACTAGAGATTCAGAGAAAATTACAATTGCGAATAGAGGAACAAGGAAAGCAGTTGAAGATGATGTTTGACCAACAACAGAAGACAAGTATAGTGGCCAATTGA
Protein sequence

>MsG0180002970.01.T01

MSRFESPTSAFYATEICMGFPQYDYQVGNESSNPLLISQFSSNKVNDLEFPLYQRENHYLDSTTNQSSHNFELSNLNTLQPILRSPEKSNKIQCGNFLGENFLSVEQNKFFIDDAASVSMSPLIHSNGNQDHKKVKRMRKFDCVLGCLIVEATPKAILRLMDSDGLTIFHVKSHLQKYRIAKYMPEQAQGNLLSYSGNPRKNSCREWNLDANRNAILFSGVGLQIREALQLQLDVQRRLHEQLEIQRKLQLRIEEQGKQLKMMFDQQQKTSIVAN*