Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003101.01.T01 | KEH26068.1 | 90.625 | 128 | 9 | 1 | 1 | 128 | 106 | 230 | 1.11E-78 | 241 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003101.01.T01 | Q8S9H7 | 53.191 | 47 | 22 | 0 | 2 | 48 | 132 | 178 | 1.43E-11 | 63.2 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003101.01.T01 | A0A072UAF3 | 90.625 | 128 | 9 | 1 | 1 | 128 | 106 | 230 | 5.29e-79 | 241 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180003101.01.T01 | TF | MYB-related |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003101.01.T01 | MTR_6g043490 | 90.625 | 128 | 9 | 1 | 1 | 128 | 106 | 230 | 1.34e-82 | 241 |
| MsG0180003101.01.T01 | MTR_6g043480 | 88.281 | 128 | 12 | 1 | 1 | 128 | 106 | 230 | 9.86e-80 | 234 |
| MsG0180003101.01.T01 | MTR_8g072990 | 67.133 | 143 | 31 | 1 | 1 | 127 | 99 | 241 | 3.31e-64 | 195 |
| MsG0180003101.01.T01 | MTR_8g073050 | 62.016 | 129 | 40 | 6 | 2 | 128 | 103 | 224 | 5.58e-42 | 138 |
| MsG0180003101.01.T01 | MTR_5g081860 | 39.744 | 78 | 42 | 2 | 2 | 79 | 125 | 197 | 8.11e-13 | 63.5 |
| MsG0180003101.01.T01 | MTR_1g048660 | 47.170 | 53 | 28 | 0 | 3 | 55 | 13 | 65 | 1.89e-12 | 59.3 |
| MsG0180003101.01.T01 | MTR_3g462790 | 51.064 | 47 | 23 | 0 | 2 | 48 | 147 | 193 | 3.13e-12 | 62.4 |
| MsG0180003101.01.T01 | MTR_5g069710 | 51.064 | 47 | 23 | 0 | 2 | 48 | 132 | 178 | 3.48e-12 | 62.0 |
| MsG0180003101.01.T01 | MTR_5g027570 | 40.541 | 74 | 41 | 1 | 2 | 75 | 92 | 162 | 3.63e-12 | 61.2 |
| MsG0180003101.01.T01 | MTR_4g111975 | 37.179 | 78 | 40 | 2 | 2 | 74 | 138 | 211 | 5.73e-12 | 61.2 |
| MsG0180003101.01.T01 | MTR_8g063870 | 31.000 | 100 | 52 | 1 | 4 | 86 | 94 | 193 | 1.52e-11 | 60.1 |
| MsG0180003101.01.T01 | MTR_2g100930 | 37.333 | 75 | 40 | 2 | 2 | 76 | 109 | 176 | 1.93e-11 | 60.1 |
| MsG0180003101.01.T01 | MTR_7g067080 | 48.936 | 47 | 24 | 0 | 2 | 48 | 140 | 186 | 3.27e-11 | 59.3 |
| MsG0180003101.01.T01 | MTR_8g101650 | 38.806 | 67 | 36 | 1 | 2 | 68 | 105 | 166 | 4.71e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003101.01.T01 | AT5G58900 | 53.191 | 47 | 22 | 0 | 2 | 48 | 141 | 187 | 9.95e-13 | 63.5 |
| MsG0180003101.01.T01 | AT2G38090 | 42.424 | 66 | 34 | 1 | 2 | 67 | 141 | 202 | 2.32e-12 | 62.4 |
| MsG0180003101.01.T01 | AT3G11280 | 47.917 | 48 | 25 | 0 | 2 | 49 | 127 | 174 | 3.71e-12 | 61.6 |
| MsG0180003101.01.T01 | AT3G11280 | 47.917 | 48 | 25 | 0 | 2 | 49 | 127 | 174 | 3.71e-12 | 61.6 |
| MsG0180003101.01.T01 | AT5G05790 | 47.917 | 48 | 25 | 0 | 2 | 49 | 131 | 178 | 7.64e-12 | 60.8 |
| MsG0180003101.01.T01 | AT5G05790 | 47.917 | 48 | 25 | 0 | 2 | 49 | 131 | 178 | 7.64e-12 | 60.8 |
| MsG0180003101.01.T01 | AT1G49010 | 46.000 | 50 | 27 | 0 | 2 | 51 | 134 | 183 | 8.37e-12 | 61.2 |
| MsG0180003101.01.T01 | AT5G01200 | 52.830 | 53 | 24 | 1 | 2 | 53 | 146 | 198 | 1.17e-11 | 60.5 |
| MsG0180003101.01.T01 | AT5G08520 | 46.000 | 50 | 27 | 0 | 2 | 51 | 118 | 167 | 5.10e-11 | 58.9 |
| MsG0180003101.01.T01 | AT5G56840 | 34.615 | 78 | 51 | 0 | 2 | 79 | 91 | 168 | 5.98e-11 | 58.2 |
Find 27 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCATAAAATGTTGGAACTTT+TGG | 0.122390 | 1:+56580089 | None:intergenic |
| ACACATATGTGCATGGTTTA+AGG | 0.242121 | 1:-56580175 | MsG0180003101.01.T01:CDS |
| GTTGATTTGGAACGTCGTTT+AGG | 0.390126 | 1:-56579978 | MsG0180003101.01.T01:CDS |
| GTGATGGGGATGATTAAGTA+TGG | 0.403039 | 1:-56580296 | MsG0180003101.01.T01:CDS |
| TGTCTTAGCTTATTAGTGAT+GGG | 0.419172 | 1:-56580311 | MsG0180003101.01.T01:intron |
| ACTCTTCCTGTTGTTTATGT+TGG | 0.423320 | 1:-56580011 | MsG0180003101.01.T01:CDS |
| GCTATGCTACTAGCTTCTTC+AGG | 0.423580 | 1:-56580208 | MsG0180003101.01.T01:CDS |
| TGGTCATGGAGAAGTTGATT+TGG | 0.426283 | 1:-56579991 | MsG0180003101.01.T01:CDS |
| TTAAGTATGGAAAAGGGAAA+TGG | 0.433708 | 1:-56580283 | MsG0180003101.01.T01:CDS |
| GGGATGATTAAGTATGGAAA+AGG | 0.439099 | 1:-56580290 | MsG0180003101.01.T01:CDS |
| ATGTGCATGGTTTAAGGAAA+AGG | 0.449777 | 1:-56580169 | MsG0180003101.01.T01:CDS |
| CCTGTTGTTTATGTTGGTCA+TGG | 0.470311 | 1:-56580005 | MsG0180003101.01.T01:CDS |
| TTGTCTTAGCTTATTAGTGA+TGG | 0.474565 | 1:-56580312 | MsG0180003101.01.T01:intron |
| AGTGCTTGCTTCATAGTTGT+TGG | 0.477998 | 1:+56580040 | None:intergenic |
| CCATGACCAACATAAACAAC+AGG | 0.483887 | 1:+56580005 | None:intergenic |
| AAACCATGCACATATGTGTC+TGG | 0.497935 | 1:+56580179 | None:intergenic |
| GTCTTAGCTTATTAGTGATG+GGG | 0.500061 | 1:-56580310 | MsG0180003101.01.T01:intron |
| GATCTAGGCGCATGGTGCCT+TGG | 0.505252 | 1:-56580897 | None:intergenic |
| ATGGTTTAAGGAAAAGGAAA+AGG | 0.505645 | 1:-56580163 | MsG0180003101.01.T01:CDS |
| GCATAGCTTTGAACTTGTTG+TGG | 0.515534 | 1:+56580224 | None:intergenic |
| GCTTCTCCTCCATAAAATGT+TGG | 0.545997 | 1:+56580080 | None:intergenic |
| GGATGATTAAGTATGGAAAA+GGG | 0.561038 | 1:-56580289 | MsG0180003101.01.T01:CDS |
| TGTTAACAATGATCTTGCAA+AGG | 0.578401 | 1:-56580129 | MsG0180003101.01.T01:CDS |
| CATGCACATATGTGTCTGGT+AGG | 0.610902 | 1:+56580183 | None:intergenic |
| ATAAACAACAGGAAGAGTCA+TGG | 0.611118 | 1:+56580016 | None:intergenic |
| TCATGCTCTAATTGACTCCA+AGG | 0.619497 | 1:+56580880 | None:intergenic |
| CTACCAGACACATATGTGCA+TGG | 0.648995 | 1:-56580182 | MsG0180003101.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAATAAAAAAAAAGATTTA+AGG | + | Chr1:56580017-56580036 | None:intergenic | 10.0% |
| !! | ACTAGAGAAATAAAAAAAAA+TGG | + | Chr1:56580132-56580151 | None:intergenic | 15.0% |
| !! | AGGTTATTAGATAATAAATT+TGG | - | Chr1:56580371-56580390 | MsG0180003101.01.T01:intron | 15.0% |
| !! | ATAATAAATTTGGTCAAAAA+AGG | - | Chr1:56580381-56580400 | MsG0180003101.01.T01:intron | 15.0% |
| !! | CTAGAGAAATAAAAAAAAAT+GGG | + | Chr1:56580131-56580150 | None:intergenic | 15.0% |
| !! | TAGAGAAATAAAAAAAAATG+GGG | + | Chr1:56580130-56580149 | None:intergenic | 15.0% |
| !!! | TTTTTTTATTTCTCTAGTAT+TGG | - | Chr1:56580134-56580153 | MsG0180003101.01.T01:CDS | 15.0% |
| ! | AATAAGTAGAAATGAGTGTA+TGG | + | Chr1:56580218-56580237 | None:intergenic | 25.0% |
| ! | TATTATCTAATAACCTACGA+AGG | + | Chr1:56580367-56580386 | None:intergenic | 25.0% |
| ! | TCTATGATAAATTGTGAGTT+TGG | + | Chr1:56580491-56580510 | None:intergenic | 25.0% |
| ATAACCTACGAAGGTTTAAT+TGG | + | Chr1:56580358-56580377 | None:intergenic | 30.0% | |
| ATGGTTTAAGGAAAAGGAAA+AGG | - | Chr1:56580691-56580710 | MsG0180003101.01.T01:intron | 30.0% | |
| GGATGATTAAGTATGGAAAA+GGG | - | Chr1:56580565-56580584 | MsG0180003101.01.T01:intron | 30.0% | |
| TGTCTTAGCTTATTAGTGAT+GGG | - | Chr1:56580543-56580562 | MsG0180003101.01.T01:intron | 30.0% | |
| TGTTAACAATGATCTTGCAA+AGG | - | Chr1:56580725-56580744 | MsG0180003101.01.T01:intron | 30.0% | |
| TTAAGTATGGAAAAGGGAAA+TGG | - | Chr1:56580571-56580590 | MsG0180003101.01.T01:intron | 30.0% | |
| TTGTCTTAGCTTATTAGTGA+TGG | - | Chr1:56580542-56580561 | MsG0180003101.01.T01:intron | 30.0% | |
| ! | AAAGTTCCAACATTTTATGG+AGG | - | Chr1:56580768-56580787 | MsG0180003101.01.T01:intron | 30.0% |
| ! | CCAAAAGTTCCAACATTTTA+TGG | - | Chr1:56580765-56580784 | MsG0180003101.01.T01:intron | 30.0% |
| ! | CCATAAAATGTTGGAACTTT+TGG | + | Chr1:56580768-56580787 | None:intergenic | 30.0% |
| AACTCCAATTAAACCTTCGT+AGG | - | Chr1:56580351-56580370 | MsG0180003101.01.T01:intron | 35.0% | |
| ACACATATGTGCATGGTTTA+AGG | - | Chr1:56580679-56580698 | MsG0180003101.01.T01:intron | 35.0% | |
| ATAAACAACAGGAAGAGTCA+TGG | + | Chr1:56580841-56580860 | None:intergenic | 35.0% | |
| ATGTGCATGGTTTAAGGAAA+AGG | - | Chr1:56580685-56580704 | MsG0180003101.01.T01:intron | 35.0% | |
| GGGATGATTAAGTATGGAAA+AGG | - | Chr1:56580564-56580583 | MsG0180003101.01.T01:intron | 35.0% | |
| GTCTTAGCTTATTAGTGATG+GGG | - | Chr1:56580544-56580563 | MsG0180003101.01.T01:intron | 35.0% | |
| ! | ACTCTTCCTGTTGTTTATGT+TGG | - | Chr1:56580843-56580862 | MsG0180003101.01.T01:intron | 35.0% |
| AAACCATGCACATATGTGTC+TGG | + | Chr1:56580678-56580697 | None:intergenic | 40.0% | |
| AGTGCTTGCTTCATAGTTGT+TGG | + | Chr1:56580817-56580836 | None:intergenic | 40.0% | |
| ATGAGTGTATGGCCAACATA+TGG | + | Chr1:56580207-56580226 | None:intergenic | 40.0% | |
| CCATGACCAACATAAACAAC+AGG | + | Chr1:56580852-56580871 | None:intergenic | 40.0% | |
| GCTTCTCCTCCATAAAATGT+TGG | + | Chr1:56580777-56580796 | None:intergenic | 40.0% | |
| TCTCATTCTGCACCATATGT+TGG | - | Chr1:56580192-56580211 | MsG0180003101.01.T01:CDS | 40.0% | |
| ! | CCTGTTGTTTATGTTGGTCA+TGG | - | Chr1:56580849-56580868 | MsG0180003101.01.T01:intron | 40.0% |
| ! | GTGATGGGGATGATTAAGTA+TGG | - | Chr1:56580558-56580577 | MsG0180003101.01.T01:intron | 40.0% |
| ! | TCATGCTCTAATTGACTCCA+AGG | + | Chr1:56579977-56579996 | None:intergenic | 40.0% |
| ! | TGGTCATGGAGAAGTTGATT+TGG | - | Chr1:56580863-56580882 | MsG0180003101.01.T01:intron | 40.0% |
| !! | GCATAGCTTTGAACTTGTTG+TGG | + | Chr1:56580633-56580652 | None:intergenic | 40.0% |
| !! | GTTGATTTGGAACGTCGTTT+AGG | - | Chr1:56580876-56580895 | MsG0180003101.01.T01:intron | 40.0% |
| CATGCACATATGTGTCTGGT+AGG | + | Chr1:56580674-56580693 | None:intergenic | 45.0% | |
| CTACCAGACACATATGTGCA+TGG | - | Chr1:56580672-56580691 | MsG0180003101.01.T01:intron | 45.0% | |
| ! | GCTATGCTACTAGCTTCTTC+AGG | - | Chr1:56580646-56580665 | MsG0180003101.01.T01:intron | 45.0% |
| !!! | AAATTTGAATAATAATTTTA+AGG | + | Chr1:56580443-56580462 | None:intergenic | 5.0% |
| !!! | AATTTGAATAATAATTTTAA+GGG | + | Chr1:56580442-56580461 | None:intergenic | 5.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 56579968 | 56580908 | 56579968 | ID=MsG0180003101.01;Name=MsG0180003101.01 |
| Chr1 | mRNA | 56579968 | 56580908 | 56579968 | ID=MsG0180003101.01.T01;Parent=MsG0180003101.01;Name=MsG0180003101.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0|0|2|0|128 |
| Chr1 | exon | 56580880 | 56580908 | 56580880 | ID=MsG0180003101.01.T01:exon:9094;Parent=MsG0180003101.01.T01 |
| Chr1 | exon | 56579968 | 56580325 | 56579968 | ID=MsG0180003101.01.T01:exon:9093;Parent=MsG0180003101.01.T01 |
| Chr1 | CDS | 56580880 | 56580908 | 56580880 | ID=MsG0180003101.01.T01:cds;Parent=MsG0180003101.01.T01 |
| Chr1 | CDS | 56579968 | 56580325 | 56579968 | ID=MsG0180003101.01.T01:cds;Parent=MsG0180003101.01.T01 |
| Gene Sequence |
| Protein sequence |
