AlfalfaGEDB | MsG0180003217.01

M. sativa cultivar ZhongmuNo.1 / MsG0180003217.01

KEGG: -
GO: GO:0003677
PFAM: PF02362

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003217.01.T01	XP_039688434.1	62.034	295	59	6	223	473	20	305	1.13E-108	340

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003217.01.T01	A0A396JPN4	62.034	295	59	6	223	473	20	305	5.40e-109	340

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TFs/TRs:

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Gene ID	Type	Classification
MsG0180003217.01.T01	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003217.01.T01	MTR_1g035570	63.571	280	49	4	223	460	20	288	4.73e-109	330
MsG0180003217.01.T01	MTR_1g035490	68.657	201	50	4	263	461	9	198	5.40e-80	252
MsG0180003217.01.T01	MTR_1g035640	46.570	277	94	2	223	460	20	281	1.18e-71	235
MsG0180003217.01.T01	MTR_1g035620	42.414	290	100	11	216	462	17	282	2.11e-52	182
MsG0180003217.01.T01	MTR_1g050845	41.724	290	90	9	216	462	17	270	7.62e-49	180
MsG0180003217.01.T01	MTR_6g061070	34.459	296	129	10	224	460	34	323	2.71e-32	129
MsG0180003217.01.T01	MTR_1g035650	34.300	207	73	5	216	379	17	203	8.72e-25	102
MsG0180003217.01.T01	MTR_3g047490	53.165	79	32	1	3	81	27	100	1.79e-17	84.0
MsG0180003217.01.T01	MTR_5g044670	24.252	301	162	8	224	474	32	316	8.80e-17	81.6
MsG0180003217.01.T01	MTR_7g050710	24.585	301	142	8	223	459	28	307	1.17e-15	78.2

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 82 sgRNAs with CRISPR-Local

Find 574 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACCTAGGAATTTCTTTG+AGG	0.431350	1:-58541531	None:intergenic
AAAGGAAAGATGCAGAAGGT+TGG	0.591290	1:+58533866	MsG0180003217.01.T01:CDS
AACATATGTGAACTTGGTCA+AGG	0.631522	1:+58532874	MsG0180003217.01.T01:CDS
AAGACAGAAGTGTCTTCTAT+TGG	0.434132	1:-58534406	None:intergenic
AAGGTAACTCACCTGAGTAG+TGG	0.583534	1:-58534444	None:intergenic
AAGTATGAAGTAAACATGGA+AGG	0.610824	1:+58532800	MsG0180003217.01.T01:CDS
AAGTGATGAAGTGAGTGATA+AGG	0.524193	1:+58540580	MsG0180003217.01.T01:CDS
AATAGTTCAAGTAGAGTCAC+CGG	0.592431	1:+58532757	MsG0180003217.01.T01:CDS
AATATCAAATCTGTTGATGA+TGG	0.577260	1:+58540497	MsG0180003217.01.T01:CDS
AATCTTGCTCTCCACTACTC+AGG	0.621202	1:+58534433	MsG0180003217.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAATCTTGTATGAGTGG+TGG	+	Chr1:58541383-58541402	MsG0180003217.01.T01:CDS	30.0%
AAAACCTAGGAATTTCTTTG+AGG	-	Chr1:58541534-58541553	None:intergenic	30.0%
AAACACAAACAAAGGATTAG+TGG	+	Chr1:58536449-58536468	MsG0180003217.01.T01:intron	30.0%
AAAGAAAGGGAAAATGATCA+TGG	+	Chr1:58532963-58532982	MsG0180003217.01.T01:intron	30.0%
AACACTTGATATACAATCAG+AGG	-	Chr1:58536972-58536991	None:intergenic	30.0%
AAGTAATATATCCAGATTCC+AGG	-	Chr1:58534851-58534870	None:intergenic	30.0%
AAGTATGAAGTAAACATGGA+AGG	+	Chr1:58532800-58532819	MsG0180003217.01.T01:CDS	30.0%
AATTAACCTGGATAGAACTT+AGG	+	Chr1:58535799-58535818	MsG0180003217.01.T01:intron	30.0%
AATTTACTTCATGGCTATTC+TGG	+	Chr1:58533284-58533303	MsG0180003217.01.T01:intron	30.0%
ACTGTTACACAAAAAAGACA+CGG	+	Chr1:58539919-58539938	MsG0180003217.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	58532734	58541598	58532734	ID=MsG0180003217.01;Name=MsG0180003217.01
Chr1	mRNA	58532734	58541598	58532734	ID=MsG0180003217.01.T01;Parent=MsG0180003217.01;Name=MsG0180003217.01.T01;_AED=0.56;_eAED=0.69;_QI=0\|0\|0\|0.75\|0.85\|1\|8\|0\|488
Chr1	exon	58532734	58532942	58532734	ID=MsG0180003217.01.T01:exon:22952;Parent=MsG0180003217.01.T01
Chr1	exon	58533820	58533919	58533820	ID=MsG0180003217.01.T01:exon:22953;Parent=MsG0180003217.01.T01
Chr1	exon	58534098	58534196	58534098	ID=MsG0180003217.01.T01:exon:22954;Parent=MsG0180003217.01.T01
Chr1	exon	58534271	58534528	58534271	ID=MsG0180003217.01.T01:exon:22955;Parent=MsG0180003217.01.T01
Chr1	exon	58540249	58540321	58540249	ID=MsG0180003217.01.T01:exon:22956;Parent=MsG0180003217.01.T01
Chr1	exon	58540438	58540701	58540438	ID=MsG0180003217.01.T01:exon:22957;Parent=MsG0180003217.01.T01
Chr1	exon	58541059	58541192	58541059	ID=MsG0180003217.01.T01:exon:22958;Parent=MsG0180003217.01.T01
Chr1	exon	58541269	58541598	58541269	ID=MsG0180003217.01.T01:exon:22959;Parent=MsG0180003217.01.T01
Chr1	CDS	58532734	58532942	58532734	ID=MsG0180003217.01.T01:cds;Parent=MsG0180003217.01.T01
Chr1	CDS	58533820	58533919	58533820	ID=MsG0180003217.01.T01:cds;Parent=MsG0180003217.01.T01
Chr1	CDS	58534098	58534196	58534098	ID=MsG0180003217.01.T01:cds;Parent=MsG0180003217.01.T01
Chr1	CDS	58534271	58534528	58534271	ID=MsG0180003217.01.T01:cds;Parent=MsG0180003217.01.T01
Chr1	CDS	58540249	58540321	58540249	ID=MsG0180003217.01.T01:cds;Parent=MsG0180003217.01.T01
Chr1	CDS	58540438	58540701	58540438	ID=MsG0180003217.01.T01:cds;Parent=MsG0180003217.01.T01
Chr1	CDS	58541059	58541192	58541059	ID=MsG0180003217.01.T01:cds;Parent=MsG0180003217.01.T01
Chr1	CDS	58541269	58541598	58541269	ID=MsG0180003217.01.T01:cds;Parent=MsG0180003217.01.T01

Gene Sequence

>MsG0180003217.01.T01

ATGGATATTCCATGGGAATCCTTAATAGTTCAAGTAGAGTCACCGGTGGATTTTGCTTCACTCAAGAAGTATGAAGTAAACATGGAAGGATTGATAGAGACTCAGATGCTGTCTGAGTATTTCAAGATGCTAAATGGGCCAACATATGTGAACTTGGTCAAGGACTTTTGGTTAAAAGCTGAAGTGTACAATGAAAGCCTGCAAAAGTCAACTAGTACTGAGTCAGAAGCTGCTAAACCTGTTTCTATCAAGGAAAAAGGAAAGATGCAGAAGGTTGGTGCTGCCTCTATTCCTCAAAAAGAAGAAAAGACAGAAAGGAACAAAGCACAATACATTCATCAAGAGATGAAAAGCTCAAGGCCTTGGATGGAAGATTGTGATAGGTTCTACAAAGATAAATTGGCTGAAAAGAGGGAACTTCAAAGTAGTTCCTCAACTGCTGATCCACAAAAGATCACTTCTGAAGCTGGCCCTGCAGATCAGGTAATTCAGATTCCAGTCACCTCTCTCCCCATTTCACCCTCTTCACCATCAGCACCTGAACCAATAGAAGACACTTCTGTCTTAGACAATCTTGCTCTCCACTACTCAGGTGAGTTACCTTTAGACAAATCTACCTCAGAAAAGGCCTCTGAAGCAACTCCAGAAGCAGCTGCTTCTGAAAATAGGGTTCCTAAGATTTATGTGGAGAAATATTGGAAAGGAATATCAAACCCTATATTCCTTAAATTCCCAAACGGTGGATCATATTTTAAGGTTAAGATATTTGGTGCCAATACTTTAGAAATAAATTATTCCAATATCAAATCTGTTGATGATGGTGCTGAAGCCACAAAAAGAGGTCAAGAAAGTGATGATCAAGCCACAAAAGAAGTTCATGAAAGTGATGAAGTGAGTGATAAGGGTTCAGATGAGGTTGAAATTCTAAAGCAAGTTCAAAGAACTAGAAATGGCAAAAGAAAAGTGAGTATGGATTTTGCCACTACCCAACAAAAATTCTCAGGTAGTAACAAAGTAGCCATGGTGAAGAAGGCTAGAAAATACCCAACATCTGAAGCTTTAATCGAGAGAGCTAATAATGAAAATCCTTATTTTGAAATTATAATGTCACATACTTACGCCAATGGTTATTACTTGTGGATACCAAGTATATTTTCAAGAGAACACTTGAACAATTTTAAAGGGACTGCAACAATAAGGGTTGGTAAAGAAAGGACTCTGGAAGTGAGTTTGAGGTATTATGATAGCAAGAAAAAATCTTGTATGAGTGGTGGTTGGAAATTATTCAACAGAAAATATAATTTGCAAGTTGATGATGTTTGCAAATTTGAGATGATTCAAAGGAAACCAATTTCATTCAATGTTACTATTACTCGTGCTCGTGCCAGAAACGAATCAACCTCAAAGAAATTCCTAGGTTTTTCTTTCTTTCTTTTTAGTTTTTTATTTGGATTTTTCTATGTTTAA

Protein sequence

>MsG0180003217.01.T01

MDIPWESLIVQVESPVDFASLKKYEVNMEGLIETQMLSEYFKMLNGPTYVNLVKDFWLKAEVYNESLQKSTSTESEAAKPVSIKEKGKMQKVGAASIPQKEEKTERNKAQYIHQEMKSSRPWMEDCDRFYKDKLAEKRELQSSSSTADPQKITSEAGPADQVIQIPVTSLPISPSSPSAPEPIEDTSVLDNLALHYSGELPLDKSTSEKASEATPEAAASENRVPKIYVEKYWKGISNPIFLKFPNGGSYFKVKIFGANTLEINYSNIKSVDDGAEATKRGQESDDQATKEVHESDEVSDKGSDEVEILKQVQRTRNGKRKVSMDFATTQQKFSGSNKVAMVKKARKYPTSEALIERANNENPYFEIIMSHTYANGYYLWIPSIFSREHLNNFKGTATIRVGKERTLEVSLRYYDSKKKSCMSGGWKLFNRKYNLQVDDVCKFEMIQRKPISFNVTITRARARNESTSKKFLGFSFFLFSFLFGFFYV*