AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003243.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003243.01.T01 MTR_1g056470 92.830 265 19 0 1 265 1 265 0.0 501
MsG0180003243.01.T01 MTR_3g018640 61.479 257 95 1 1 253 1 257 4.84e-112 325
MsG0180003243.01.T01 MTR_6g065170 59.108 269 104 3 1 263 1 269 6.42e-104 305
MsG0180003243.01.T01 MTR_8g106940 57.621 269 108 2 1 263 1 269 1.95e-103 304
MsG0180003243.01.T01 MTR_3g053170 67.273 220 72 0 34 253 26 245 5.67e-103 302
MsG0180003243.01.T01 MTR_5g093460 59.144 257 100 3 1 253 130 385 1.20e-99 298
MsG0180003243.01.T01 MTR_5g093460 68.182 132 41 1 30 161 1 131 2.94e-53 179
MsG0180003243.01.T01 MTR_1g043540 59.664 119 47 1 138 256 9 126 4.66e-42 140
MsG0180003243.01.T01 MTR_1g043500 47.619 63 32 1 192 253 3 65 4.28e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003243.01.T01 AT3G20800 61.776 259 92 3 1 253 1 258 1.03e-103 304
MsG0180003243.01.T01 AT5G12980 60.784 255 97 2 1 253 1 254 3.76e-100 295
MsG0180003243.01.T01 AT2G32550 35.185 216 126 4 49 253 67 279 1.77e-35 129
MsG0180003243.01.T01 AT2G32550 35.122 205 119 4 60 253 39 240 4.84e-34 124
MsG0180003243.01.T01 AT2G32550 36.306 157 97 2 100 253 29 185 1.72e-29 111

Find 48 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCTTCATTTGCTCTCTA+AGG 0.219918 1:-58970967 MsG0180003243.01.T01:intron
ATGAATGAAAACTTTGTGTT+AGG 0.318229 1:+58969250 None:intergenic
TGCCTACGCAATATGGAAAT+TGG 0.348006 1:-58968119 MsG0180003243.01.T01:CDS
GCCGTTGTGCCTACGCAATA+TGG 0.352059 1:-58968126 MsG0180003243.01.T01:CDS
CAATACTCTTGCTCTACTTC+AGG 0.377796 1:-58969432 MsG0180003243.01.T01:intron
CCGTTATTGTGGACTTCAAT+TGG 0.388452 1:-58969945 MsG0180003243.01.T01:CDS
AGCAGTTAAGATGGTGGAGT+TGG 0.389913 1:+58967749 None:intergenic
AGAAAGGGATACAAATATAT+AGG 0.395209 1:+58968395 None:intergenic
GATTTAAGACTTGCCAGTCT+TGG 0.402589 1:-58968341 MsG0180003243.01.T01:CDS
CTTACTTGATGTATTAAGAA+AGG 0.404067 1:+58968379 None:intergenic
TAAGATGGTGGAGTTGGGTT+CGG 0.419375 1:+58967755 None:intergenic
TTACTTGATGTATTAAGAAA+GGG 0.448647 1:+58968380 None:intergenic
TTCTTCAAGGAGGGTGATTG+AGG 0.449123 1:+58971095 None:intergenic
TTTGTCAGATGATGATGGTT+TGG 0.451681 1:-58967930 MsG0180003243.01.T01:CDS
GAGGAAGGAAGCAGTTAAGA+TGG 0.457991 1:+58967740 None:intergenic
GTGCTGGAGGCAGATGTAAC+AGG 0.465456 1:+58971059 None:intergenic
CATCTTGTTGCCAATGGTGC+TGG 0.465484 1:+58971043 None:intergenic
TGAATGAAAACTTTGTGTTA+GGG 0.469491 1:+58969251 None:intergenic
CAGTTGGTCGAGCTTTGGAC+AGG 0.472419 1:-58967874 MsG0180003243.01.T01:CDS
GCAAGTCTTAAATCTTCAAA+TGG 0.486164 1:+58968350 None:intergenic
ACATCTGCCTCCAGCACCAT+TGG 0.494495 1:-58971053 MsG0180003243.01.T01:CDS
GCAGTTAAGATGGTGGAGTT+GGG 0.497274 1:+58967750 None:intergenic
AAGAACTCGCACCGTTATTG+TGG 0.516389 1:-58969956 MsG0180003243.01.T01:CDS
TCTTCAAGGAGGGTGATTGA+GGG 0.516540 1:+58971096 None:intergenic
GGCAGCAATGACTCCAAGAC+TGG 0.526684 1:+58968328 None:intergenic
TCGAGCTTTGGACAGGATGT+TGG 0.529344 1:-58967867 MsG0180003243.01.T01:CDS
ATATTCACAGGTTAAGACTA+AGG 0.533680 1:-58968183 MsG0180003243.01.T01:intron
AGTCATTGCTGCCATGGTAA+AGG 0.543352 1:-58968318 MsG0180003243.01.T01:intron
GAAGGAAGCAGTTAAGATGG+TGG 0.550389 1:+58967743 None:intergenic
CCAATTGAAGTCCACAATAA+CGG 0.550671 1:+58969945 None:intergenic
TCTTGGAGTCATTGCTGCCA+TGG 0.555856 1:-58968324 MsG0180003243.01.T01:CDS
AATTCATTATTCTTCAAGGA+GGG 0.558130 1:+58971086 None:intergenic
TTCAAGGAGGGTGATTGAGG+GGG 0.561905 1:+58971098 None:intergenic
AGCTTTAAAAGACGAGGTGA+AGG 0.570450 1:+58967827 None:intergenic
ATGAAAACTTTGTGTTAGGG+TGG 0.574572 1:+58969254 None:intergenic
TCAAGGAGGGTGATTGAGGG+GGG 0.591467 1:+58971099 None:intergenic
GCATTACTTCAGTTGCGAGA+AGG 0.595531 1:+58968147 None:intergenic
TGCCAATTTCCATATTGCGT+AGG 0.604311 1:+58968117 None:intergenic
ATACAACATCTTGTTGCCAA+TGG 0.605197 1:+58971037 None:intergenic
CTTGTTGCCAATGGTGCTGG+AGG 0.622571 1:+58971046 None:intergenic
TGAAAACTTTGTGTTAGGGT+GGG 0.623871 1:+58969255 None:intergenic
TCCATATTGCGTAGGCACAA+CGG 0.628181 1:+58968125 None:intergenic
GTTAAGACTAAGGAAGCGAT+AGG 0.628788 1:-58968173 MsG0180003243.01.T01:CDS
AAATTCATTATTCTTCAAGG+AGG 0.632490 1:+58971085 None:intergenic
ATAGAATAATACCTTTACCA+TGG 0.642599 1:+58968307 None:intergenic
CTTCAAGGAGGGTGATTGAG+GGG 0.647804 1:+58971097 None:intergenic
ATCATAAGCTTTAAAAGACG+AGG 0.660763 1:+58967821 None:intergenic
TTTGTGAGACTTGTATAACA+CGG 0.661280 1:+58967797 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTGAGTAAAAAAATATAAA+TGG + Chr1:58968093-58968112 None:intergenic 10.0%
!! TTGAGTAAAAAAATATAAAT+GGG + Chr1:58968092-58968111 None:intergenic 10.0%
!!! ATTTTTTATATGAAATACAA+GGG - Chr1:58970331-58970350 MsG0180003243.01.T01:intron 10.0%
!!! GAAAAATGAAAATTTTAATT+AGG - Chr1:58968198-58968217 MsG0180003243.01.T01:intron 10.0%
!!! TATTTTTTATATGAAATACA+AGG - Chr1:58970330-58970349 MsG0180003243.01.T01:intron 10.0%
!! AAAAAATTCATTATTCTTCA+AGG + Chr1:58967785-58967804 None:intergenic 15.0%
!! AAATATTTGTGTTAACAAAT+AGG + Chr1:58969088-58969107 None:intergenic 15.0%
!! ACCAAAAAAAAAAAAAAAGA+AGG + Chr1:58970060-58970079 None:intergenic 15.0%
!! TAAGAAGAAAATAAAAACAA+CGG + Chr1:58969530-58969549 None:intergenic 15.0%
!! TGAGTAAAAAAATATAAATG+GGG + Chr1:58968091-58968110 None:intergenic 15.0%
!!! AAATTTAAAATTTCACTTCA+TGG + Chr1:58969337-58969356 None:intergenic 15.0%
!!! AATTTTTTATTATAGTGAAG+TGG + Chr1:58968561-58968580 None:intergenic 15.0%
!!! ATATATATCATTGATTTCAA+TGG - Chr1:58968694-58968713 MsG0180003243.01.T01:intron 15.0%
!!! ATTTTATTAGAGAATAAAGA+AGG + Chr1:58970095-58970114 None:intergenic 15.0%
!!! ATTTTTTATTATAGTGAAGT+GGG + Chr1:58968560-58968579 None:intergenic 15.0%
!!! ATTTTTTTTTTCATGATTGA+TGG - Chr1:58970578-58970597 MsG0180003243.01.T01:intron 15.0%
!!! GACTTTTTTTTGTATAAAAA+TGG - Chr1:58970115-58970134 MsG0180003243.01.T01:intron 15.0%
!!! TTTTTTATATGAAATACAAG+GGG - Chr1:58970332-58970351 MsG0180003243.01.T01:intron 15.0%
!! AAATATGTCACATAAATTGA+TGG - Chr1:58969938-58969957 MsG0180003243.01.T01:CDS 20.0%
!! ATTTATAATTGCTAGAAATG+AGG - Chr1:58969851-58969870 MsG0180003243.01.T01:intron 20.0%
!! ATTTCATTAGGAGTTATAAT+TGG + Chr1:58970872-58970891 None:intergenic 20.0%
!! GAACAATGAAATATGAAATT+AGG - Chr1:58968511-58968530 MsG0180003243.01.T01:intron 20.0%
!! TGGAAACAAAAAACATAAAA+TGG + Chr1:58969113-58969132 None:intergenic 20.0%
!! TTAAATAGTTCACTCTATAT+TGG - Chr1:58969228-58969247 MsG0180003243.01.T01:intron 20.0%
!! TTAACGCATATAAGAATATT+TGG + Chr1:58968318-58968337 None:intergenic 20.0%
!! TTACTTGATGTATTAAGAAA+GGG + Chr1:58970487-58970506 None:intergenic 20.0%
!! TTATTTACAGTAGAATTTCT+AGG - Chr1:58969006-58969025 MsG0180003243.01.T01:intron 20.0%
!! TTCTTACAAAATATACCAAA+TGG + Chr1:58968120-58968139 None:intergenic 20.0%
!! TTGATAAGTACAATAATAGT+TGG + Chr1:58969684-58969703 None:intergenic 20.0%
!! TTTATAATTGCTAGAAATGA+GGG - Chr1:58969852-58969871 MsG0180003243.01.T01:intron 20.0%
!!! ATTCTTTTATTAACTAGCTT+TGG - Chr1:58968338-58968357 MsG0180003243.01.T01:CDS 20.0%
!!! CTTTATTTTTCTTTCTCTTT+TGG - Chr1:58969062-58969081 MsG0180003243.01.T01:intron 20.0%
!!! GCCTTCTTTTTTTTTTTTTT+TGG - Chr1:58970056-58970075 MsG0180003243.01.T01:intron 20.0%
!!! TACTATTGCAATATTTTTAC+AGG - Chr1:58968942-58968961 MsG0180003243.01.T01:intron 20.0%
!!! TGAAATTTTAAATTTCTCTC+AGG - Chr1:58969342-58969361 MsG0180003243.01.T01:intron 20.0%
!!! TGGTATATTTTGTAAGAATT+AGG - Chr1:58968122-58968141 MsG0180003243.01.T01:CDS 20.0%
!!! TGTAGTTTTTATCAAAATTG+TGG + Chr1:58970812-58970831 None:intergenic 20.0%
! AAACAAATTAAGCATTAGCA+AGG + Chr1:58969263-58969282 None:intergenic 25.0%
! AAATTCATTATTCTTCAAGG+AGG + Chr1:58967782-58967801 None:intergenic 25.0%
! AATTCATTATTCTTCAAGGA+GGG + Chr1:58967781-58967800 None:intergenic 25.0%
! AGAAAGGGATACAAATATAT+AGG + Chr1:58970472-58970491 None:intergenic 25.0%
! AGTGATATACACATTTCATT+AGG + Chr1:58970884-58970903 None:intergenic 25.0%
! ATAGAATAATACCTTTACCA+TGG + Chr1:58970560-58970579 None:intergenic 25.0%
! ATGAATGAAAACTTTGTGTT+AGG + Chr1:58969617-58969636 None:intergenic 25.0%
! ATGAGTGGAATAATATCTAA+CGG + Chr1:58969977-58969996 None:intergenic 25.0%
! CATACATCTTTCAATTTATG+GGG - Chr1:58967928-58967947 MsG0180003243.01.T01:CDS 25.0%
! CCATAAATTGAAAGATGTAT+GGG + Chr1:58967929-58967948 None:intergenic 25.0%
! CCATACATCTTTCAATTTAT+GGG - Chr1:58967927-58967946 MsG0180003243.01.T01:CDS 25.0%
! CTTACTTGATGTATTAAGAA+AGG + Chr1:58970488-58970507 None:intergenic 25.0%
! GAAGAAATACATGTAGTTAA+AGG - Chr1:58968439-58968458 MsG0180003243.01.T01:intron 25.0%
! GAATATCATGTATCAACAAA+TGG + Chr1:58970667-58970686 None:intergenic 25.0%
! GATAAAAAAGTTTAGATGAG+TGG + Chr1:58969992-58970011 None:intergenic 25.0%
! GTTTCATCATGATAAAATCA+TGG + Chr1:58968413-58968432 None:intergenic 25.0%
! TAAATGTGAATACTCTCTAT+GGG - Chr1:58968029-58968048 MsG0180003243.01.T01:intron 25.0%
! TGAATGAAAACTTTGTGTTA+GGG + Chr1:58969616-58969635 None:intergenic 25.0%
! TTAAATGTGAATACTCTCTA+TGG - Chr1:58968028-58968047 MsG0180003243.01.T01:intron 25.0%
!! AAAATTTTGTCAGATGATGA+TGG - Chr1:58970929-58970948 MsG0180003243.01.T01:intron 25.0%
!! AACAAGATACAAAACTTGTT+AGG + Chr1:58969472-58969491 None:intergenic 25.0%
!! ACAAAGTTTTCATTCATGAA+AGG - Chr1:58969620-58969639 MsG0180003243.01.T01:intron 25.0%
!! AGTTTTTATCAAAATTGTGG+TGG + Chr1:58970809-58970828 None:intergenic 25.0%
!! ATTGGTAACTAAATTACAAG+TGG + Chr1:58970854-58970873 None:intergenic 25.0%
!! ATTTTGTAAGAATTAGGATG+CGG - Chr1:58968128-58968147 MsG0180003243.01.T01:CDS 25.0%
!! TCTTTTACAATTGTGCTTAT+TGG - Chr1:58969297-58969316 MsG0180003243.01.T01:intron 25.0%
!! TTAAGTTGTTAGTATCTTCA+TGG + Chr1:58969761-58969780 None:intergenic 25.0%
!! TTTTACTCAATTCAACCATT+TGG - Chr1:58968102-58968121 MsG0180003243.01.T01:CDS 25.0%
!!! AATCCATTTCTAAAATCACT+TGG - Chr1:58968732-58968751 MsG0180003243.01.T01:intron 25.0%
!!! ATATAGCTTTTTGACTCATT+TGG + Chr1:58968162-58968181 None:intergenic 25.0%
!!! ATCCATTTCTAAAATCACTT+GGG - Chr1:58968733-58968752 MsG0180003243.01.T01:intron 25.0%
!!! CAAAGCTTTGAATGATATAT+AGG + Chr1:58968245-58968264 None:intergenic 25.0%
!!! CAAGTTTTGTATCTTGTTTA+AGG - Chr1:58969474-58969493 MsG0180003243.01.T01:CDS 25.0%
!!! CATCATTTTTCCTCATAAAA+AGG - Chr1:58970376-58970395 MsG0180003243.01.T01:intron 25.0%
!!! TATAGCTTTTTGACTCATTT+GGG + Chr1:58968161-58968180 None:intergenic 25.0%
!!! TTCTCTCTAATTTATTTTGC+AGG - Chr1:58970199-58970218 MsG0180003243.01.T01:intron 25.0%
ACAAGTCTCACAAAAAATGT+AGG - Chr1:58971076-58971095 MsG0180003243.01.T01:CDS 30.0%
ATATTCACAGGTTAAGACTA+AGG - Chr1:58970681-58970700 MsG0180003243.01.T01:intron 30.0%
CAAGTCTCACAAAAAATGTA+GGG - Chr1:58971077-58971096 MsG0180003243.01.T01:CDS 30.0%
CCCATAAATTGAAAGATGTA+TGG + Chr1:58967930-58967949 None:intergenic 30.0%
CCCATACATCTTTCAATTTA+TGG - Chr1:58967926-58967945 MsG0180003243.01.T01:CDS 30.0%
GAATTGTGAAAGTAAAAGGT+TGG + Chr1:58969133-58969152 None:intergenic 30.0%
GACAATGAATATGAGGAAAA+AGG - Chr1:58968481-58968500 MsG0180003243.01.T01:intron 30.0%
GAGGGAAAGTTAAAACTAAA+TGG - Chr1:58969177-58969196 MsG0180003243.01.T01:intron 30.0%
GCAAGTCTTAAATCTTCAAA+TGG + Chr1:58970517-58970536 None:intergenic 30.0%
GTTACAATCTTGTTAGTACT+TGG - Chr1:58968803-58968822 MsG0180003243.01.T01:intron 30.0%
GTTGGAATTGTGAAAGTAAA+AGG + Chr1:58969137-58969156 None:intergenic 30.0%
TAATTTACCACTATCCTTCA+AGG - Chr1:58969816-58969835 MsG0180003243.01.T01:intron 30.0%
TATCCTTCAAGGAAATTTGT+TGG - Chr1:58969827-58969846 MsG0180003243.01.T01:intron 30.0%
TGAAATGTGTATATCACTAC+AGG - Chr1:58970886-58970905 MsG0180003243.01.T01:intron 30.0%
TGTTAGCTGCATATAAGAAA+AGG + Chr1:58970447-58970466 None:intergenic 30.0%
TGTTGATACATGATATTCAC+AGG - Chr1:58970669-58970688 MsG0180003243.01.T01:intron 30.0%
TTTGTGAGACTTGTATAACA+CGG + Chr1:58971070-58971089 None:intergenic 30.0%
! ATCCTTCAAGGAAATTTGTT+GGG - Chr1:58969828-58969847 MsG0180003243.01.T01:intron 30.0%
! TCAAATGTTGCCTTTTTATG+AGG + Chr1:58970389-58970408 None:intergenic 30.0%
!! AACCCAAGTGATTTTAGAAA+TGG + Chr1:58968738-58968757 None:intergenic 30.0%
!! ATCATAAGCTTTAAAAGACG+AGG + Chr1:58971046-58971065 None:intergenic 30.0%
!! TAACTAGCTTTGGATAACTA+TGG - Chr1:58968348-58968367 MsG0180003243.01.T01:CDS 30.0%
ACTTAAACCAGATGACTACA+TGG + Chr1:58968592-58968611 None:intergenic 35.0%
AGAGTGTGACAATGAATATG+AGG - Chr1:58968474-58968493 MsG0180003243.01.T01:intron 35.0%
AGTGGAATAATATCTAACGG+AGG + Chr1:58969974-58969993 None:intergenic 35.0%
ATACAACATCTTGTTGCCAA+TGG + Chr1:58967830-58967849 None:intergenic 35.0%
ATAGTGTGTGTGAACTTGTA+TGG + Chr1:58968007-58968026 None:intergenic 35.0%
ATCAAAATTGTGGTGGTTCA+TGG + Chr1:58970802-58970821 None:intergenic 35.0%
ATCCCAACAAATTTCCTTGA+AGG + Chr1:58969833-58969852 None:intergenic 35.0%
ATGAAAACTTTGTGTTAGGG+TGG + Chr1:58969613-58969632 None:intergenic 35.0%
ATGTGATCGACAATGTGAAT+TGG - Chr1:58968626-58968645 MsG0180003243.01.T01:intron 35.0%
ATTTCATCCATGTAGTCATC+TGG - Chr1:58968582-58968601 MsG0180003243.01.T01:intron 35.0%
CAAATTTCCTTGAAGGATAG+TGG + Chr1:58969826-58969845 None:intergenic 35.0%
CCAATTGAAGTCCACAATAA+CGG + Chr1:58968922-58968941 None:intergenic 35.0%
GGATATACCAACTACAAAGT+AGG - Chr1:58970136-58970155 MsG0180003243.01.T01:intron 35.0%
GTGGAATAATATCTAACGGA+GGG + Chr1:58969973-58969992 None:intergenic 35.0%
TAGTGTGTGTGAACTTGTAT+GGG + Chr1:58968006-58968025 None:intergenic 35.0%
TCGTCAATACAAAGTCACTT+TGG + Chr1:58969159-58969178 None:intergenic 35.0%
TGAAAACTTTGTGTTAGGGT+GGG + Chr1:58969612-58969631 None:intergenic 35.0%
TGTGATCGACAATGTGAATT+GGG - Chr1:58968627-58968646 MsG0180003243.01.T01:intron 35.0%
! AAAGTGACTTTGTATTGACG+AGG - Chr1:58969158-58969177 MsG0180003243.01.T01:intron 35.0%
! AAGTGACTTTGTATTGACGA+GGG - Chr1:58969159-58969178 MsG0180003243.01.T01:intron 35.0%
! CAATACAAAGTCACTTTGGT+TGG + Chr1:58969155-58969174 None:intergenic 35.0%
! CATGATTTTGTTACAGTGTG+TGG - Chr1:58969583-58969602 MsG0180003243.01.T01:intron 35.0%
!! CTCAAACTACACATTGACTT+GGG - Chr1:58969895-58969914 MsG0180003243.01.T01:intron 35.0%
!! TTTGTCAGATGATGATGGTT+TGG - Chr1:58970934-58970953 MsG0180003243.01.T01:intron 35.0%
!!! AAGAGCATTTTCTCGTAGAT+CGG + Chr1:58967884-58967903 None:intergenic 35.0%
CAATACTCTTGCTCTACTTC+AGG - Chr1:58969432-58969451 MsG0180003243.01.T01:intron 40.0%
CCAACTACAAAGTAGGTCTT+AGG - Chr1:58970143-58970162 MsG0180003243.01.T01:intron 40.0%
CCAACTGCAAAAAAACGATC+TGG + Chr1:58970977-58970996 None:intergenic 40.0%
CCGTTATTGTGGACTTCAAT+TGG - Chr1:58968919-58968938 MsG0180003243.01.T01:intron 40.0%
CCTAAGACCTACTTTGTAGT+TGG + Chr1:58970146-58970165 None:intergenic 40.0%
GAATAATATCTAACGGAGGG+AGG + Chr1:58969970-58969989 None:intergenic 40.0%
GGGTTAGAAACCAAACAAAC+AGG - Chr1:58969872-58969891 MsG0180003243.01.T01:intron 40.0%
GTAGTTTGAGCCTGTTTGTT+TGG + Chr1:58969885-58969904 None:intergenic 40.0%
GTTAAGACTAAGGAAGCGAT+AGG - Chr1:58970691-58970710 MsG0180003243.01.T01:intron 40.0%
TGCCAATTTCCATATTGCGT+AGG + Chr1:58970750-58970769 None:intergenic 40.0%
TGCCTACGCAATATGGAAAT+TGG - Chr1:58970745-58970764 MsG0180003243.01.T01:intron 40.0%
TGCTCTTCATTTGCTCTCTA+AGG - Chr1:58967897-58967916 MsG0180003243.01.T01:CDS 40.0%
TGTGTGAACTTGTATGGGAT+TGG + Chr1:58968001-58968020 None:intergenic 40.0%
TTCACTTCATGGTAAGTGCA+AGG + Chr1:58969326-58969345 None:intergenic 40.0%
! GATTTAAGACTTGCCAGTCT+TGG - Chr1:58970523-58970542 MsG0180003243.01.T01:intron 40.0%
! GCTCAAACTACACATTGACT+TGG - Chr1:58969894-58969913 MsG0180003243.01.T01:intron 40.0%
! TGTGCAGCAGTAAGATTTTC+TGG + Chr1:58969397-58969416 None:intergenic 40.0%
!! AGAGCATTTTCTCGTAGATC+GGG + Chr1:58967883-58967902 None:intergenic 40.0%
!! AGATTTTCTGGTGAAAGAGC+AGG + Chr1:58969385-58969404 None:intergenic 40.0%
!! AGCTTTAAAAGACGAGGTGA+AGG + Chr1:58971040-58971059 None:intergenic 40.0%
!!! CCAGATCGTTTTTTTGCAGT+TGG - Chr1:58970974-58970993 MsG0180003243.01.T01:CDS 40.0%
AAGAACTCGCACCGTTATTG+TGG - Chr1:58968908-58968927 MsG0180003243.01.T01:intron 45.0%
AGTCATTGCTGCCATGGTAA+AGG - Chr1:58970546-58970565 MsG0180003243.01.T01:intron 45.0%
AGTCTCGTAACATGGTGCAT+CGG + Chr1:58968066-58968085 None:intergenic 45.0%
GCATTACTTCAGTTGCGAGA+AGG + Chr1:58970720-58970739 None:intergenic 45.0%
TCCATATTGCGTAGGCACAA+CGG + Chr1:58970742-58970761 None:intergenic 45.0%
TCTTCAAGGAGGGTGATTGA+GGG + Chr1:58967771-58967790 None:intergenic 45.0%
TTCTTCAAGGAGGGTGATTG+AGG + Chr1:58967772-58967791 None:intergenic 45.0%
! GAGCATTTTCTCGTAGATCG+GGG + Chr1:58967882-58967901 None:intergenic 45.0%
! TTTTGCAGTTGGTCGAGCTT+TGG - Chr1:58970985-58971004 MsG0180003243.01.T01:CDS 45.0%
!! TAAGATGGTGGAGTTGGGTT+CGG + Chr1:58971112-58971131 None:intergenic 45.0%
!! AAAATTATAATATTTATGTA+TGG + Chr1:58969503-58969522 None:intergenic 5.0%
CATCTTGTTGCCAATGGTGC+TGG + Chr1:58967824-58967843 None:intergenic 50.0%
CTTCAAGGAGGGTGATTGAG+GGG + Chr1:58967770-58967789 None:intergenic 50.0%
TCTTGGAGTCATTGCTGCCA+TGG - Chr1:58970540-58970559 MsG0180003243.01.T01:intron 50.0%
TTCAAGGAGGGTGATTGAGG+GGG + Chr1:58967769-58967788 None:intergenic 50.0%
!! ATGGGGCTAGTCTCGTAACA+TGG + Chr1:58968074-58968093 None:intergenic 50.0%
!! TCGAGCTTTGGACAGGATGT+TGG - Chr1:58970997-58971016 MsG0180003243.01.T01:CDS 50.0%
ACATCTGCCTCCAGCACCAT+TGG - Chr1:58967811-58967830 MsG0180003243.01.T01:CDS 55.0%
GCCGTTGTGCCTACGCAATA+TGG - Chr1:58970738-58970757 MsG0180003243.01.T01:intron 55.0%
GGCAGCAATGACTCCAAGAC+TGG + Chr1:58970539-58970558 None:intergenic 55.0%
GTGCTGGAGGCAGATGTAAC+AGG + Chr1:58967808-58967827 None:intergenic 55.0%
TCAAGGAGGGTGATTGAGGG+GGG + Chr1:58967768-58967787 None:intergenic 55.0%
! CTTGTTGCCAATGGTGCTGG+AGG + Chr1:58967821-58967840 None:intergenic 55.0%
!! CAGTTGGTCGAGCTTTGGAC+AGG - Chr1:58970990-58971009 MsG0180003243.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 58967754 58971132 58967754 ID=MsG0180003243.01;Name=MsG0180003243.01
Chr1 mRNA 58967754 58971132 58967754 ID=MsG0180003243.01.T01;Parent=MsG0180003243.01;Name=MsG0180003243.01.T01;_AED=0.36;_eAED=0.37;_QI=0|0|0|1|1|1|7|0|265
Chr1 exon 58970968 58971132 58970968 ID=MsG0180003243.01.T01:exon:49100;Parent=MsG0180003243.01.T01
Chr1 exon 58969923 58969994 58969923 ID=MsG0180003243.01.T01:exon:49099;Parent=MsG0180003243.01.T01
Chr1 exon 58969433 58969522 58969433 ID=MsG0180003243.01.T01:exon:49098;Parent=MsG0180003243.01.T01
Chr1 exon 58969245 58969287 58969245 ID=MsG0180003243.01.T01:exon:49097;Parent=MsG0180003243.01.T01
Chr1 exon 58968319 58968425 58968319 ID=MsG0180003243.01.T01:exon:49096;Parent=MsG0180003243.01.T01
Chr1 exon 58968100 58968195 58968100 ID=MsG0180003243.01.T01:exon:49095;Parent=MsG0180003243.01.T01
Chr1 exon 58967754 58967978 58967754 ID=MsG0180003243.01.T01:exon:49094;Parent=MsG0180003243.01.T01
Chr1 CDS 58970968 58971132 58970968 ID=MsG0180003243.01.T01:cds;Parent=MsG0180003243.01.T01
Chr1 CDS 58969923 58969994 58969923 ID=MsG0180003243.01.T01:cds;Parent=MsG0180003243.01.T01
Chr1 CDS 58969433 58969522 58969433 ID=MsG0180003243.01.T01:cds;Parent=MsG0180003243.01.T01
Chr1 CDS 58969245 58969287 58969245 ID=MsG0180003243.01.T01:cds;Parent=MsG0180003243.01.T01
Chr1 CDS 58968319 58968425 58968319 ID=MsG0180003243.01.T01:cds;Parent=MsG0180003243.01.T01
Chr1 CDS 58968100 58968195 58968100 ID=MsG0180003243.01.T01:cds;Parent=MsG0180003243.01.T01
Chr1 CDS 58967754 58967978 58967754 ID=MsG0180003243.01.T01:cds;Parent=MsG0180003243.01.T01
Gene Sequence

>MsG0180003243.01.T01

ATGGCGAATTTCCCCCCTCAATCACCCTCCTTGAAGAATAATGAATTTTTTCCTGTTACATCTGCCTCCAGCACCATTGGCAACAAGATGTTGTATGCTGAGCGTCTTGTTATTGAACTGAGCAACCCCGATCTACGAGAAAATGCTCTTCATTTGCTCTCTAAGATGACAGATTTGTTTCAAGAACTCGCACCGTTATTGTGGACTTCAATTGGTACTATTGCAATATTTTTACAGGAAATAATTACAATATATCCTGCTCTTTCACCAGAAAATCTTACTGCTGCACAATCAACTCGAATATGCAATACTCTTGCTCTACTTCAGTGTGTGGCTTCCCACCCTAACACAAAGTTTTCATTCATGAAAGCTAACATACCTATATATTTGTATCCCTTTCTTAATACATCAAGTAAGTTAACTCCATTTGAAGATTTAAGACTTGCCAGTCTTGGAGTCATTGCTGCCATGGTAAAGGTTAAGACTAAGGAAGCGATAGGCTTCCTTCTCGCAACTGAAGTAATGCCGTTGTGCCTACGCAATATGGAAATTGGCAAAGAATTGTCAAAAACTGTTGCAACATTTATCGTTGAGAAAATTTTGTCAGATGATGATGGTTTGGCGTATGTTTGTGCTACACCAGATCGTTTTTTTGCAGTTGGTCGAGCTTTGGACAGGATGTTGGCAAGTGTTGACAATCAACCTTCACCTCGTCTTTTAAAGCTTATGATTCCGTGTTATACAAGTCTCACAAAAAATGTAGGGTTGTTTAAGCCGAACCCAACTCCACCATCTTAA
Protein sequence

>MsG0180003243.01.T01

MANFPPQSPSLKNNEFFPVTSASSTIGNKMLYAERLVIELSNPDLRENALHLLSKMTDLFQELAPLLWTSIGTIAIFLQEIITIYPALSPENLTAAQSTRICNTLALLQCVASHPNTKFSFMKANIPIYLYPFLNTSSKLTPFEDLRLASLGVIAAMVKVKTKEAIGFLLATEVMPLCLRNMEIGKELSKTVATFIVEKILSDDDGLAYVCATPDRFFAVGRALDRMLASVDNQPSPRLLKLMIPCYTSLTKNVGLFKPNPTPPS*