AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003247.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003247.01.T01 MTR_1g056810 88.396 293 11 2 1 275 1 288 0.0 507
MsG0180003247.01.T01 MTR_3g090430 54.610 141 38 4 3 142 41 156 1.60e-41 145
MsG0180003247.01.T01 MTR_2g093220 63.441 93 28 2 13 105 41 127 6.21e-32 119
MsG0180003247.01.T01 MTR_3g091820 87.037 54 7 0 13 66 44 97 2.06e-29 113
MsG0180003247.01.T01 MTR_8g027295 87.037 54 7 0 13 66 25 78 2.69e-29 112
MsG0180003247.01.T01 MTR_8g479350 81.034 58 11 0 8 65 64 121 1.15e-28 112
MsG0180003247.01.T01 MTR_4g088580 59.406 101 27 4 14 105 57 152 1.17e-28 112
MsG0180003247.01.T01 MTR_4g461080 90.196 51 5 0 14 64 31 81 2.17e-28 110
MsG0180003247.01.T01 MTR_4g063780 75.862 58 14 0 14 71 77 134 4.18e-28 110
MsG0180003247.01.T01 MTR_1g077600 75.410 61 15 0 6 66 37 97 4.55e-28 108
MsG0180003247.01.T01 MTR_1g077600 75.410 61 15 0 6 66 37 97 5.80e-28 109
MsG0180003247.01.T01 MTR_7g059400 81.132 53 10 0 14 66 78 130 1.30e-27 109
MsG0180003247.01.T01 MTR_8g068210 75.862 58 14 0 9 66 29 86 2.24e-27 108
MsG0180003247.01.T01 MTR_2g096740 77.358 53 12 0 14 66 79 131 2.85e-27 107
MsG0180003247.01.T01 MTR_2g096740 77.358 53 12 0 14 66 64 116 3.30e-27 107
MsG0180003247.01.T01 MTR_7g024670 79.310 58 9 1 14 68 79 136 3.63e-27 108
MsG0180003247.01.T01 MTR_5g031440 87.755 49 6 0 14 62 45 93 1.76e-26 106
MsG0180003247.01.T01 MTR_3g077750 81.132 53 10 0 10 62 59 111 2.76e-26 105
MsG0180003247.01.T01 MTR_2g014060 82.353 51 9 0 14 64 27 77 3.67e-26 105
MsG0180003247.01.T01 MTR_4g022370 79.245 53 11 0 14 66 84 136 3.96e-26 105
MsG0180003247.01.T01 MTR_4g109980 85.714 49 7 0 14 62 42 90 2.66e-25 102
MsG0180003247.01.T01 MTR_8g015840 80.000 50 10 0 14 63 22 71 1.20e-24 98.6
MsG0180003247.01.T01 MTR_4g089095 75.926 54 12 1 13 66 51 103 1.88e-24 100
MsG0180003247.01.T01 MTR_4g089095 75.926 54 12 1 13 66 17 69 2.38e-24 99.0
MsG0180003247.01.T01 MTR_2g014170 85.106 47 7 0 14 60 47 93 3.29e-24 99.4
MsG0180003247.01.T01 MTR_2g059540 78.431 51 11 0 10 60 29 79 3.40e-24 97.1
MsG0180003247.01.T01 MTR_3g435480 66.129 62 20 1 14 75 138 198 4.66e-24 101
MsG0180003247.01.T01 MTR_8g079060 73.585 53 14 0 14 66 31 83 4.85e-24 97.4
MsG0180003247.01.T01 MTR_2g013370 74.074 54 13 1 13 66 47 99 7.10e-24 97.8
MsG0180003247.01.T01 MTR_4g082060 46.789 109 56 2 6 114 117 223 2.14e-23 99.4
MsG0180003247.01.T01 MTR_7g010950 51.064 94 42 3 6 99 130 219 3.04e-23 99.0
MsG0180003247.01.T01 MTR_6g012450 50.000 100 41 4 6 99 131 227 5.93e-23 98.2
MsG0180003247.01.T01 MTR_2g016030 64.516 62 21 1 4 65 40 100 3.57e-22 90.5
MsG0180003247.01.T01 MTR_5g041420 61.667 60 22 1 6 65 133 191 1.80e-21 92.4
MsG0180003247.01.T01 MTR_7g086780 50.562 89 37 2 14 102 101 182 1.92e-21 93.2
MsG0180003247.01.T01 MTR_8g044220 59.016 61 24 1 6 66 113 172 3.47e-21 92.8
MsG0180003247.01.T01 MTR_5g041530 60.938 64 24 1 2 65 131 193 2.70e-20 89.7
MsG0180003247.01.T01 MTR_6g027450 62.264 53 20 0 14 66 91 143 4.15e-20 88.6
MsG0180003247.01.T01 MTR_6g027460 65.385 52 18 0 14 65 98 149 5.42e-20 88.6
MsG0180003247.01.T01 MTR_5g041380 58.462 65 26 1 1 65 117 180 9.84e-20 88.2
MsG0180003247.01.T01 MTR_5g041400 65.385 52 18 0 14 65 121 172 4.01e-19 86.3
MsG0180003247.01.T02 MTR_1g056810 94.539 293 11 1 1 293 1 288 0.0 552
MsG0180003247.01.T02 MTR_3g090430 54.610 141 38 4 3 142 41 156 1.63e-41 146
MsG0180003247.01.T02 MTR_2g093220 63.441 93 28 2 13 105 41 127 6.48e-32 120
MsG0180003247.01.T02 MTR_3g091820 87.037 54 7 0 13 66 44 97 2.47e-29 113
MsG0180003247.01.T02 MTR_8g027295 87.037 54 7 0 13 66 25 78 3.31e-29 112
MsG0180003247.01.T02 MTR_4g088580 58.416 101 28 3 14 105 57 152 1.10e-28 113
MsG0180003247.01.T02 MTR_8g479350 81.034 58 11 0 8 65 64 121 1.16e-28 112
MsG0180003247.01.T02 MTR_4g461080 90.196 51 5 0 14 64 31 81 2.49e-28 110
MsG0180003247.01.T02 MTR_4g063780 75.862 58 14 0 14 71 77 134 4.11e-28 110
MsG0180003247.01.T02 MTR_1g077600 75.410 61 15 0 6 66 37 97 5.53e-28 109
MsG0180003247.01.T02 MTR_1g077600 75.410 61 15 0 6 66 37 97 5.67e-28 109
MsG0180003247.01.T02 MTR_7g059400 81.132 53 10 0 14 66 78 130 1.33e-27 109
MsG0180003247.01.T02 MTR_8g068210 75.862 58 14 0 9 66 29 86 2.40e-27 108
MsG0180003247.01.T02 MTR_2g096740 77.358 53 12 0 14 66 64 116 3.22e-27 107
MsG0180003247.01.T02 MTR_2g096740 77.358 53 12 0 14 66 79 131 3.50e-27 107
MsG0180003247.01.T02 MTR_7g024670 79.310 58 9 1 14 68 79 136 3.83e-27 108
MsG0180003247.01.T02 MTR_5g031440 87.755 49 6 0 14 62 45 93 1.83e-26 106
MsG0180003247.01.T02 MTR_3g077750 81.132 53 10 0 10 62 59 111 3.41e-26 105
MsG0180003247.01.T02 MTR_2g014060 84.000 50 8 0 14 63 27 76 3.97e-26 105
MsG0180003247.01.T02 MTR_4g022370 79.245 53 11 0 14 66 84 136 4.71e-26 105
MsG0180003247.01.T02 MTR_4g109980 85.714 49 7 0 14 62 42 90 3.03e-25 102
MsG0180003247.01.T02 MTR_8g015840 80.000 50 10 0 14 63 22 71 1.33e-24 99.0
MsG0180003247.01.T02 MTR_4g089095 75.926 54 12 1 13 66 51 103 1.99e-24 100
MsG0180003247.01.T02 MTR_4g089095 75.926 54 12 1 13 66 17 69 2.28e-24 99.4
MsG0180003247.01.T02 MTR_2g014170 85.106 47 7 0 14 60 47 93 3.73e-24 99.8
MsG0180003247.01.T02 MTR_3g435480 66.129 62 20 1 14 75 138 198 3.89e-24 101
MsG0180003247.01.T02 MTR_2g059540 78.431 51 11 0 10 60 29 79 3.91e-24 97.4
MsG0180003247.01.T02 MTR_8g079060 73.585 53 14 0 14 66 31 83 4.41e-24 97.8
MsG0180003247.01.T02 MTR_2g013370 74.074 54 13 1 13 66 47 99 6.85e-24 98.6
MsG0180003247.01.T02 MTR_4g082060 46.789 109 56 2 6 114 117 223 2.31e-23 99.8
MsG0180003247.01.T02 MTR_7g010950 51.064 94 42 3 6 99 130 219 2.90e-23 99.4
MsG0180003247.01.T02 MTR_6g012450 50.000 100 41 4 6 99 131 227 4.67e-23 99.0
MsG0180003247.01.T02 MTR_2g016030 64.516 62 21 1 4 65 40 100 4.07e-22 90.9
MsG0180003247.01.T02 MTR_5g041420 61.667 60 22 1 6 65 133 191 1.74e-21 92.8
MsG0180003247.01.T02 MTR_7g086780 50.562 89 37 2 14 102 101 182 1.87e-21 94.0
MsG0180003247.01.T02 MTR_8g044220 59.016 61 24 1 6 66 113 172 3.58e-21 93.2
MsG0180003247.01.T02 MTR_5g041530 60.938 64 24 1 2 65 131 193 2.50e-20 90.1
MsG0180003247.01.T02 MTR_6g027450 62.264 53 20 0 14 66 91 143 4.10e-20 89.0
MsG0180003247.01.T02 MTR_6g027460 65.385 52 18 0 14 65 98 149 5.45e-20 89.4
MsG0180003247.01.T02 MTR_5g041380 58.462 65 26 1 1 65 117 180 9.31e-20 88.6
MsG0180003247.01.T02 MTR_5g041400 65.385 52 18 0 14 65 121 172 4.11e-19 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003247.01.T01 AT3G52440 38.028 284 114 10 5 275 13 247 8.71e-49 162
MsG0180003247.01.T01 AT3G52440 38.028 284 114 10 5 275 32 266 1.18e-48 162
MsG0180003247.01.T01 AT1G21340 37.681 207 101 6 11 216 37 216 6.72e-34 124
MsG0180003247.01.T01 AT3G61850 77.273 66 15 0 1 66 51 116 3.82e-31 117
MsG0180003247.01.T01 AT3G61850 77.273 66 15 0 1 66 51 116 3.82e-31 117
MsG0180003247.01.T01 AT3G61850 77.273 66 15 0 1 66 63 128 5.44e-31 117
MsG0180003247.01.T01 AT3G61850 77.273 66 15 0 1 66 51 116 5.59e-31 117
MsG0180003247.01.T01 AT5G60200 81.034 58 11 0 9 66 52 109 5.90e-31 116
MsG0180003247.01.T01 AT3G61850 77.273 66 15 0 1 66 63 128 8.29e-31 117
MsG0180003247.01.T01 AT4G24060 88.889 54 6 0 13 66 54 107 4.31e-30 115
MsG0180003247.01.T01 AT2G28510 66.667 75 25 0 13 87 49 123 1.63e-29 113
MsG0180003247.01.T01 AT1G07640 80.000 60 11 1 14 72 79 138 2.37e-29 114
MsG0180003247.01.T01 AT1G07640 80.000 60 11 1 14 72 23 82 2.73e-29 112
MsG0180003247.01.T01 AT1G07640 80.000 60 11 1 14 72 87 146 3.34e-29 113
MsG0180003247.01.T01 AT2G37590 84.906 53 8 0 14 66 91 143 2.30e-28 111
MsG0180003247.01.T01 AT4G00940 85.185 54 8 0 13 66 69 122 9.26e-28 108
MsG0180003247.01.T01 AT4G00940 85.185 54 8 0 13 66 69 122 9.26e-28 108
MsG0180003247.01.T01 AT4G00940 85.185 54 8 0 13 66 69 122 9.26e-28 108
MsG0180003247.01.T01 AT3G55370 76.271 59 14 0 13 71 77 135 1.00e-27 109
MsG0180003247.01.T01 AT3G55370 76.271 59 14 0 13 71 122 180 1.07e-27 110
MsG0180003247.01.T01 AT3G55370 76.271 59 14 0 13 71 77 135 1.24e-27 109
MsG0180003247.01.T01 AT5G60850 68.056 72 23 0 11 82 50 121 1.49e-27 108
MsG0180003247.01.T01 AT3G45610 44.444 135 48 5 14 145 42 152 2.59e-27 106
MsG0180003247.01.T01 AT5G02460 81.132 53 10 0 14 66 97 149 3.36e-27 109
MsG0180003247.01.T01 AT5G65590 79.245 53 11 0 14 66 43 95 3.64e-27 107
MsG0180003247.01.T01 AT2G46590 82.759 58 9 1 3 59 58 115 4.35e-27 108
MsG0180003247.01.T01 AT2G46590 93.617 47 3 0 13 59 81 127 5.36e-27 108
MsG0180003247.01.T01 AT2G28810 82.000 50 9 0 14 63 79 128 7.60e-27 107
MsG0180003247.01.T01 AT2G28810 82.000 50 9 0 14 63 96 145 1.06e-26 107
MsG0180003247.01.T01 AT1G28310 81.132 53 10 0 14 66 29 81 2.34e-26 105
MsG0180003247.01.T01 AT1G28310 81.132 53 10 0 14 66 53 105 3.08e-26 105
MsG0180003247.01.T01 AT5G62940 85.714 49 7 0 14 62 75 123 6.97e-26 105
MsG0180003247.01.T01 AT3G21270 84.314 51 8 0 14 64 31 81 7.21e-26 101
MsG0180003247.01.T01 AT1G51700 77.358 53 12 0 14 66 35 87 7.76e-26 101
MsG0180003247.01.T01 AT4G21050 77.358 53 12 0 14 66 26 78 1.05e-25 101
MsG0180003247.01.T01 AT1G64620 87.755 49 6 0 13 61 50 98 2.21e-25 103
MsG0180003247.01.T01 AT3G50410 75.472 53 13 0 14 66 32 84 2.50e-24 98.6
MsG0180003247.01.T01 AT4G38000 71.429 56 16 0 7 62 36 91 1.33e-23 96.7
MsG0180003247.01.T01 AT1G29160 61.538 65 24 1 1 65 52 115 2.30e-23 94.0
MsG0180003247.01.T01 AT1G69570 75.000 52 13 0 14 65 134 185 3.68e-23 98.2
MsG0180003247.01.T01 AT2G34140 71.154 52 15 0 14 65 60 111 1.42e-22 92.0
MsG0180003247.01.T01 AT5G66940 68.519 54 17 0 13 66 33 86 1.46e-22 93.2
MsG0180003247.01.T01 AT5G62430 45.455 99 50 2 6 104 49 143 1.56e-22 94.7
MsG0180003247.01.T01 AT3G47500 66.667 60 19 1 6 65 105 163 1.60e-22 96.7
MsG0180003247.01.T01 AT4G21040 75.000 52 13 0 14 65 27 78 1.74e-22 93.2
MsG0180003247.01.T01 AT4G21080 57.333 75 31 1 14 87 27 101 2.13e-22 93.6
MsG0180003247.01.T01 AT1G47655 78.723 47 10 0 14 60 31 77 2.53e-22 92.4
MsG0180003247.01.T01 AT1G26790 65.000 60 20 1 6 65 106 164 4.91e-22 94.7
MsG0180003247.01.T01 AT5G39660 66.667 60 19 1 6 65 133 191 7.84e-22 94.7
MsG0180003247.01.T01 AT5G39660 66.667 60 19 1 6 65 133 191 7.84e-22 94.7
MsG0180003247.01.T01 AT5G39660 66.667 60 19 1 6 65 133 191 7.84e-22 94.7
MsG0180003247.01.T01 AT4G21030 60.000 50 20 0 14 63 23 72 5.87e-14 69.3
MsG0180003247.01.T02 AT3G52440 35.880 301 115 7 5 293 32 266 4.97e-46 156
MsG0180003247.01.T02 AT3G52440 35.880 301 115 7 5 293 13 247 5.67e-46 155
MsG0180003247.01.T02 AT1G21340 39.409 203 91 6 11 213 37 207 9.41e-34 124
MsG0180003247.01.T02 AT5G60200 43.293 164 63 4 9 170 52 187 3.72e-31 117
MsG0180003247.01.T02 AT3G61850 77.273 66 15 0 1 66 51 116 4.58e-31 117
MsG0180003247.01.T02 AT3G61850 77.273 66 15 0 1 66 51 116 4.58e-31 117
MsG0180003247.01.T02 AT3G61850 77.273 66 15 0 1 66 63 128 6.60e-31 117
MsG0180003247.01.T02 AT3G61850 77.273 66 15 0 1 66 51 116 6.65e-31 118
MsG0180003247.01.T02 AT3G61850 77.273 66 15 0 1 66 63 128 9.48e-31 117
MsG0180003247.01.T02 AT4G24060 88.889 54 6 0 13 66 54 107 5.62e-30 116
MsG0180003247.01.T02 AT2G28510 66.667 75 25 0 13 87 49 123 1.52e-29 114
MsG0180003247.01.T02 AT1G07640 80.000 60 11 1 14 72 79 138 2.47e-29 114
MsG0180003247.01.T02 AT1G07640 80.000 60 11 1 14 72 23 82 2.91e-29 112
MsG0180003247.01.T02 AT1G07640 80.000 60 11 1 14 72 87 146 3.37e-29 114
MsG0180003247.01.T02 AT2G37590 84.906 53 8 0 14 66 91 143 2.69e-28 111
MsG0180003247.01.T02 AT4G00940 85.185 54 8 0 13 66 69 122 1.09e-27 108
MsG0180003247.01.T02 AT4G00940 85.185 54 8 0 13 66 69 122 1.09e-27 108
MsG0180003247.01.T02 AT4G00940 85.185 54 8 0 13 66 69 122 1.09e-27 108
MsG0180003247.01.T02 AT3G55370 76.271 59 14 0 13 71 77 135 1.12e-27 109
MsG0180003247.01.T02 AT3G55370 76.271 59 14 0 13 71 122 180 1.24e-27 110
MsG0180003247.01.T02 AT3G55370 76.271 59 14 0 13 71 77 135 1.37e-27 110
MsG0180003247.01.T02 AT5G60850 68.056 72 23 0 11 82 50 121 1.48e-27 108
MsG0180003247.01.T02 AT3G45610 44.444 135 48 5 14 145 42 152 3.30e-27 106
MsG0180003247.01.T02 AT5G02460 81.132 53 10 0 14 66 97 149 3.55e-27 109
MsG0180003247.01.T02 AT5G65590 79.245 53 11 0 14 66 43 95 3.68e-27 108
MsG0180003247.01.T02 AT2G46590 82.759 58 9 1 3 59 58 115 5.42e-27 108
MsG0180003247.01.T02 AT2G46590 93.617 47 3 0 13 59 81 127 5.93e-27 108
MsG0180003247.01.T02 AT2G28810 82.000 50 9 0 14 63 79 128 6.93e-27 107
MsG0180003247.01.T02 AT2G28810 82.000 50 9 0 14 63 96 145 1.07e-26 107
MsG0180003247.01.T02 AT1G28310 81.132 53 10 0 14 66 29 81 2.33e-26 105
MsG0180003247.01.T02 AT1G28310 81.132 53 10 0 14 66 53 105 3.08e-26 105
MsG0180003247.01.T02 AT1G51700 79.630 54 10 1 14 66 35 88 7.05e-26 101
MsG0180003247.01.T02 AT3G21270 84.314 51 8 0 14 64 31 81 7.61e-26 102
MsG0180003247.01.T02 AT5G62940 85.714 49 7 0 14 62 75 123 7.72e-26 105
MsG0180003247.01.T02 AT4G21050 77.358 53 12 0 14 66 26 78 1.09e-25 101
MsG0180003247.01.T02 AT1G64620 87.755 49 6 0 13 61 50 98 2.66e-25 103
MsG0180003247.01.T02 AT3G50410 75.472 53 13 0 14 66 32 84 2.57e-24 99.0
MsG0180003247.01.T02 AT4G38000 71.429 56 16 0 7 62 36 91 1.35e-23 97.1
MsG0180003247.01.T02 AT1G29160 61.538 65 24 1 1 65 52 115 2.55e-23 94.4
MsG0180003247.01.T02 AT1G69570 75.000 52 13 0 14 65 134 185 4.04e-23 98.2
MsG0180003247.01.T02 AT3G47500 66.667 60 19 1 6 65 105 163 1.36e-22 97.4
MsG0180003247.01.T02 AT5G62430 45.455 99 50 2 6 104 49 143 1.41e-22 95.5
MsG0180003247.01.T02 AT5G66940 68.519 54 17 0 13 66 33 86 1.46e-22 93.6
MsG0180003247.01.T02 AT2G34140 71.154 52 15 0 14 65 60 111 1.49e-22 92.4
MsG0180003247.01.T02 AT4G21040 75.000 52 13 0 14 65 27 78 1.75e-22 93.6
MsG0180003247.01.T02 AT4G21080 57.333 75 31 1 14 87 27 101 2.23e-22 94.0
MsG0180003247.01.T02 AT1G47655 78.723 47 10 0 14 60 31 77 2.80e-22 92.4
MsG0180003247.01.T02 AT1G26790 65.000 60 20 1 6 65 106 164 4.44e-22 95.1
MsG0180003247.01.T02 AT5G39660 66.667 60 19 1 6 65 133 191 7.28e-22 95.5
MsG0180003247.01.T02 AT5G39660 66.667 60 19 1 6 65 133 191 7.28e-22 95.5
MsG0180003247.01.T02 AT5G39660 66.667 60 19 1 6 65 133 191 7.28e-22 95.5
MsG0180003247.01.T02 AT4G21030 60.000 50 20 0 14 63 23 72 5.07e-14 69.7

Find 51 sgRNAs with CRISPR-Local

Find 63 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAACCTCCACCACCATTTCT+TGG 0.233577 1:+59062870 MsG0180003247.01.T02:CDS
GAACCACATCTTGGACAATT+TGG 0.236842 1:-59062117 None:intergenic
CAGACTTGTTAATCGGTAAT+TGG 0.248583 1:+59062913 MsG0180003247.01.T02:CDS
TCTAAAGAAGTGTGAGAATT+AGG 0.294412 1:-59062342 None:intergenic
GTTGTGGATGGTAATTGTTC+TGG 0.295167 1:-59062648 None:intergenic
ACCAATGCAAGATCAATATT+TGG 0.306644 1:-59062453 None:intergenic
GTTAATCATCATTTCAGAAT+TGG 0.308733 1:-59062829 None:intergenic
GAAATATTAGTCCTCAAAAT+AGG 0.320066 1:-59062399 None:intergenic
ATCCTTGTCTAGAAAATTCA+AGG 0.352452 1:+59062544 MsG0180003247.01.T02:CDS
CATGATGCAGACTTGTTAAT+CGG 0.365024 1:+59062906 MsG0180003247.01.T02:CDS
TGATCGGAACTAAGAGAATC+AGG 0.377500 1:-59062501 None:intergenic
GCAAAGGATGTAGAAGGTAT+TGG 0.381998 1:+59062199 MsG0180003247.01.T02:CDS
TTCATCTTCTAGCTCCTCAT+TGG 0.412030 1:+59062419 MsG0180003247.01.T02:CDS
GCATCCCTTGGAAAATCAAA+AGG 0.439777 1:-59062939 None:intergenic
ACCAAATATTGATCTTGCAT+TGG 0.456881 1:+59062452 MsG0180003247.01.T02:CDS
TGTAGAAGGTATTGGACAAA+AGG 0.461975 1:+59062207 MsG0180003247.01.T02:CDS
CTCAGAAATGTTCCTGTTGG+TGG 0.463083 1:+59062237 MsG0180003247.01.T02:CDS
GGAGGTTGAGTGACTTGAAA+TGG 0.465039 1:-59062855 None:intergenic
AATTAGAACTTGGTTTCAAA+GGG 0.465903 1:+59062610 MsG0180003247.01.T02:CDS
TTTCTGCAGCCACCACCAAC+AGG 0.485924 1:-59062249 None:intergenic
AATTTGTCTGAATTAGAACT+TGG 0.496339 1:+59062600 MsG0180003247.01.T02:CDS
TCTGCATCATGAATTTGATC+AGG 0.499238 1:-59062894 None:intergenic
GTGTTTGAAGAACCACATCT+TGG 0.501582 1:-59062126 None:intergenic
AGAAATGTTCCTGTTGGTGG+TGG 0.504532 1:+59062240 MsG0180003247.01.T02:CDS
TTTGAAAGTGAGGGAGTCAA+AGG 0.507069 1:-59062313 None:intergenic
ATTTGATCAGGACCAAGAAA+TGG 0.507530 1:-59062882 None:intergenic
GAATCCTCTTCACCTGGTAA+AGG 0.513411 1:-59062780 None:intergenic
GAATTAGAACTTGGTTTCAA+AGG 0.520155 1:+59062609 MsG0180003247.01.T02:CDS
ACTAAAATGTGTTACTGTTG+TGG 0.520461 1:-59062664 None:intergenic
AGAATCAATTAAGCATCCCT+TGG 0.523237 1:-59062951 None:intergenic
TTTGAGCTACCACCTTTACC+AGG 0.542560 1:+59062768 MsG0180003247.01.T02:CDS
GGACCAAGAAATGGTGGTGG+AGG 0.554806 1:-59062873 None:intergenic
CATCCCTTGGAAAATCAAAA+GGG 0.555636 1:-59062938 None:intergenic
ACCACCTTTACCAGGTGAAG+AGG 0.557951 1:+59062776 MsG0180003247.01.T02:CDS
TCAAGGCTATCGATTGTTGA+AGG 0.562523 1:+59062561 MsG0180003247.01.T02:CDS
TCCTCTTCACCTGGTAAAGG+TGG 0.570460 1:-59062777 None:intergenic
CACCAACAGGAACATTTCTG+AGG 0.582266 1:-59062236 None:intergenic
AGAAGGTATTGGACAAAAGG+TGG 0.588429 1:+59062210 MsG0180003247.01.T02:CDS
ATGCAGACATGGAGAGTGAG+TGG 0.591147 1:+59062076 None:intergenic
TCCCTCAGAAATGTTCCTGT+TGG 0.593993 1:+59062234 MsG0180003247.01.T02:CDS
TGGTCTATCATTCACCAATG+AGG 0.596861 1:-59062433 None:intergenic
GTTGAGTGACTTGAAATGGA+AGG 0.605356 1:-59062851 None:intergenic
CATCACAAGATCATGTATTG+TGG 0.612487 1:+59062805 MsG0180003247.01.T02:CDS
TCAGGACCAAGAAATGGTGG+TGG 0.616492 1:-59062876 None:intergenic
ACCAACAGGAACATTTCTGA+GGG 0.622749 1:-59062235 None:intergenic
TCACCAAATTGTCCAAGATG+TGG 0.624013 1:+59062114 MsG0180003247.01.T02:CDS
TGATCAGGACCAAGAAATGG+TGG 0.649067 1:-59062879 None:intergenic
AAATGTGTTACTGTTGTGGA+TGG 0.658240 1:-59062660 None:intergenic
GATGATGAATCCTCTTCACC+TGG 0.665791 1:-59062786 None:intergenic
ACTACAGCTTAACTCAACCA+AGG 0.682302 1:+59062169 MsG0180003247.01.T02:CDS
AGGCTATCGATTGTTGAAGG+CGG 0.697199 1:+59062564 MsG0180003247.01.T02:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATCAATTCTATCTTTTTGAA+GGG - Chr1:59062709-59062728 None:intergenic 20.0%
! AATTAGAACTTGGTTTCAAA+GGG + Chr1:59062610-59062629 MsG0180003247.01.T02:CDS 25.0%
! AATTTGTCTGAATTAGAACT+TGG + Chr1:59062600-59062619 MsG0180003247.01.T02:CDS 25.0%
! GAAATATTAGTCCTCAAAAT+AGG - Chr1:59062402-59062421 None:intergenic 25.0%
! GTTAATCATCATTTCAGAAT+TGG - Chr1:59062832-59062851 None:intergenic 25.0%
!! ATTCTTATGATCCTATTTTG+AGG + Chr1:59062388-59062407 MsG0180003247.01.T02:CDS 25.0%
!! GATCAATTCTATCTTTTTGA+AGG - Chr1:59062710-59062729 None:intergenic 25.0%
!!! ATTTGTTTTTCGCATTTGAT+CGG - Chr1:59062520-59062539 None:intergenic 25.0%
ACCAAATATTGATCTTGCAT+TGG + Chr1:59062452-59062471 MsG0180003247.01.T02:CDS 30.0%
ACCAATGCAAGATCAATATT+TGG - Chr1:59062456-59062475 None:intergenic 30.0%
ATCCTTGTCTAGAAAATTCA+AGG + Chr1:59062544-59062563 MsG0180003247.01.T02:CDS 30.0%
GAATTAGAACTTGGTTTCAA+AGG + Chr1:59062609-59062628 MsG0180003247.01.T02:CDS 30.0%
! ACTAAAATGTGTTACTGTTG+TGG - Chr1:59062667-59062686 None:intergenic 30.0%
! ATTTTTGCAAAGGATGTAGA+AGG + Chr1:59062193-59062212 MsG0180003247.01.T02:CDS 30.0%
! TCTAAAGAAGTGTGAGAATT+AGG - Chr1:59062345-59062364 None:intergenic 30.0%
!! AGGTGAATTTTTGAAAGTGA+GGG - Chr1:59062325-59062344 None:intergenic 30.0%
!! TAGGTGAATTTTTGAAAGTG+AGG - Chr1:59062326-59062345 None:intergenic 30.0%
ATTTGATCAGGACCAAGAAA+TGG - Chr1:59062885-59062904 None:intergenic 35.0%
CATCACAAGATCATGTATTG+TGG + Chr1:59062805-59062824 MsG0180003247.01.T02:CDS 35.0%
CATCCCTTGGAAAATCAAAA+GGG - Chr1:59062941-59062960 None:intergenic 35.0%
CATCCTTTGCAAAAATACCT+TGG - Chr1:59062189-59062208 None:intergenic 35.0%
TCTGCATCATGAATTTGATC+AGG - Chr1:59062897-59062916 None:intergenic 35.0%
! AAATGTGTTACTGTTGTGGA+TGG - Chr1:59062663-59062682 None:intergenic 35.0%
! AGCCTTGAATTTTCTAGACA+AGG - Chr1:59062549-59062568 None:intergenic 35.0%
!! CAACCAAGGTATTTTTGCAA+AGG + Chr1:59062183-59062202 MsG0180003247.01.T02:CDS 35.0%
!! CAGACTTGTTAATCGGTAAT+TGG + Chr1:59062913-59062932 MsG0180003247.01.T02:CDS 35.0%
!! CATGATGCAGACTTGTTAAT+CGG + Chr1:59062906-59062925 MsG0180003247.01.T02:CDS 35.0%
!! TGTAGAAGGTATTGGACAAA+AGG + Chr1:59062207-59062226 MsG0180003247.01.T02:CDS 35.0%
ACCAACAGGAACATTTCTGA+GGG - Chr1:59062238-59062257 None:intergenic 40.0%
ACTACAGCTTAACTCAACCA+AGG + Chr1:59062169-59062188 MsG0180003247.01.T02:CDS 40.0%
GAACCACATCTTGGACAATT+TGG - Chr1:59062120-59062139 None:intergenic 40.0%
GCATCCCTTGGAAAATCAAA+AGG - Chr1:59062942-59062961 None:intergenic 40.0%
GTGTTTGAAGAACCACATCT+TGG - Chr1:59062129-59062148 None:intergenic 40.0%
GTTGAGTGACTTGAAATGGA+AGG - Chr1:59062854-59062873 None:intergenic 40.0%
GTTGTGGATGGTAATTGTTC+TGG - Chr1:59062651-59062670 None:intergenic 40.0%
TCACCAAATTGTCCAAGATG+TGG + Chr1:59062114-59062133 MsG0180003247.01.T02:CDS 40.0%
TGATCGGAACTAAGAGAATC+AGG - Chr1:59062504-59062523 None:intergenic 40.0%
TGGTCTATCATTCACCAATG+AGG - Chr1:59062436-59062455 None:intergenic 40.0%
TTTGAAAGTGAGGGAGTCAA+AGG - Chr1:59062316-59062335 None:intergenic 40.0%
! GCAAAGGATGTAGAAGGTAT+TGG + Chr1:59062199-59062218 MsG0180003247.01.T02:CDS 40.0%
! TCAAGGCTATCGATTGTTGA+AGG + Chr1:59062561-59062580 MsG0180003247.01.T02:CDS 40.0%
!! AGAAGGTATTGGACAAAAGG+TGG + Chr1:59062210-59062229 MsG0180003247.01.T02:CDS 40.0%
!! GAGAATCAGGCTTTTGATTG+AGG - Chr1:59062491-59062510 None:intergenic 40.0%
!! TTCATCTTCTAGCTCCTCAT+TGG + Chr1:59062419-59062438 MsG0180003247.01.T02:CDS 40.0%
!!! GAGCCCTTTTGATTTTCCAA+GGG + Chr1:59062935-59062954 MsG0180003247.01.T02:CDS 40.0%
CACCAACAGGAACATTTCTG+AGG - Chr1:59062239-59062258 None:intergenic 45.0%
CTCAGAAATGTTCCTGTTGG+TGG + Chr1:59062237-59062256 MsG0180003247.01.T02:CDS 45.0%
GAATCCTCTTCACCTGGTAA+AGG - Chr1:59062783-59062802 None:intergenic 45.0%
GATGATGAATCCTCTTCACC+TGG - Chr1:59062789-59062808 None:intergenic 45.0%
GGAGGTTGAGTGACTTGAAA+TGG - Chr1:59062858-59062877 None:intergenic 45.0%
GGCTGCAGAAAAAACAGAAG+AGG + Chr1:59062261-59062280 MsG0180003247.01.T02:CDS 45.0%
TCCCTCAGAAATGTTCCTGT+TGG + Chr1:59062234-59062253 MsG0180003247.01.T02:CDS 45.0%
TGATCAGGACCAAGAAATGG+TGG - Chr1:59062882-59062901 None:intergenic 45.0%
TTTGAGCTACCACCTTTACC+AGG + Chr1:59062768-59062787 MsG0180003247.01.T02:CDS 45.0%
! AGAAATGTTCCTGTTGGTGG+TGG + Chr1:59062240-59062259 MsG0180003247.01.T02:CDS 45.0%
! AGGCTATCGATTGTTGAAGG+CGG + Chr1:59062564-59062583 MsG0180003247.01.T02:CDS 45.0%
!!! GGAGCCCTTTTGATTTTCCA+AGG + Chr1:59062934-59062953 MsG0180003247.01.T02:CDS 45.0%
ACCACCTTTACCAGGTGAAG+AGG + Chr1:59062776-59062795 MsG0180003247.01.T02:CDS 50.0%
CAACCTCCACCACCATTTCT+TGG + Chr1:59062870-59062889 MsG0180003247.01.T02:CDS 50.0%
TCAGGACCAAGAAATGGTGG+TGG - Chr1:59062879-59062898 None:intergenic 50.0%
TCCTCTTCACCTGGTAAAGG+TGG - Chr1:59062780-59062799 None:intergenic 50.0%
TTTCTGCAGCCACCACCAAC+AGG - Chr1:59062252-59062271 None:intergenic 55.0%
!! GGACCAAGAAATGGTGGTGG+AGG - Chr1:59062876-59062895 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 59062084 59062965 59062084 ID=MsG0180003247.01;Name=MsG0180003247.01
Chr1 mRNA 59062084 59062965 59062084 ID=MsG0180003247.01.T01;Parent=MsG0180003247.01;Name=MsG0180003247.01.T01;_AED=0.04;_eAED=0.04;_QI=0|0|0|1|0|0|2|0|275
Chr1 exon 59062084 59062576 59062084 ID=MsG0180003247.01.T01:exon:3859;Parent=MsG0180003247.01.T01
Chr1 exon 59062631 59062965 59062631 ID=MsG0180003247.01.T01:exon:3860;Parent=MsG0180003247.01.T01
Chr1 CDS 59062084 59062576 59062084 ID=MsG0180003247.01.T01:cds;Parent=MsG0180003247.01.T01
Chr1 CDS 59062631 59062965 59062631 ID=MsG0180003247.01.T01:cds;Parent=MsG0180003247.01.T01
Chr1 mRNA 59062084 59062965 59062084 ID=MsG0180003247.01.T02;Parent=MsG0180003247.01;Name=MsG0180003247.01.T02;_AED=0.01;_eAED=0.01;_QI=0|-1|0|1|-1|1|1|0|293
Chr1 exon 59062084 59062965 59062084 ID=MsG0180003247.01.T02:exon:3861;Parent=MsG0180003247.01.T02
Chr1 CDS 59062084 59062965 59062084 ID=MsG0180003247.01.T02:cds;Parent=MsG0180003247.01.T02
Gene Sequence

>MsG0180003247.01.T01

ATGGAGAGTGAGTGGAAAGCTAATCAGATATCACCAAATTGTCCAAGATGTGGTTCTTCAAACACAAAATTTTGCTACTACAATAACTACAGCTTAACTCAACCAAGGTATTTTTGCAAAGGATGTAGAAGGTATTGGACAAAAGGTGGATCCCTCAGAAATGTTCCTGTTGGTGGTGGCTGCAGAAAAAACAGAAGAGGTAACAACAAAACATTAGTAAGACATAGATCCTTTGACTCCCTCACTTTCAAAAATTCACCTAATTCTCACACTTCTTTAGATAACAATAACTCAATTTCTCATCATTCTTATGATCCTATTTTGAGGACTAATATTTCATCTTCTAGCTCCTCATTGGTGAATGATAGACCAAATATTGATCTTGCATTGGTTTATGCAAATTTCCTCAATCAAAAGCCTGATTCTCTTAGTTCCGATCAAATGCGAAAAACAAATTTTGATCCTTGTCTAGAAAATTCAAGGCTATCGATTGGGTGTTTGAATCTTAATCCAGAACAATTACCATCCACAACAGTAACACATTTTAGTGAGTTCAATTCTATGCAAACCCTTCAAAAAGATAGAATTGATCATTGTAGTAATATTCATGATGATGCTGTGAATGTGAATTTTGAGCTACCACCTTTACCAGGTGAAGAGGATTCATCATCACAAGATCATGTATTGTGGTCCAATTCTGAAATGATGATTAACCTTCCATTTCAAGTCACTCAACCTCCACCACCATTTCTTGGTCCTGATCAAATTCATGATGCAGACTTGTTAATCGGTAATTGGAGCCCTTTTGATTTTCCAAGGGATGCTTAA

>MsG0180003247.01.T02

ATGGAGAGTGAGTGGAAAGCTAATCAGATATCACCAAATTGTCCAAGATGTGGTTCTTCAAACACAAAATTTTGCTACTACAATAACTACAGCTTAACTCAACCAAGGTATTTTTGCAAAGGATGTAGAAGGTATTGGACAAAAGGTGGATCCCTCAGAAATGTTCCTGTTGGTGGTGGCTGCAGAAAAAACAGAAGAGGTAACAACAAAACATTAGTAAGACATAGATCCTTTGACTCCCTCACTTTCAAAAATTCACCTAATTCTCACACTTCTTTAGATAACAATAACTCAATTTCTCATCATTCTTATGATCCTATTTTGAGGACTAATATTTCATCTTCTAGCTCCTCATTGGTGAATGATAGACCAAATATTGATCTTGCATTGGTTTATGCAAATTTCCTCAATCAAAAGCCTGATTCTCTTAGTTCCGATCAAATGCGAAAAACAAATTTTGATCCTTGTCTAGAAAATTCAAGGCTATCGATTGTTGAAGGCGGTACGAGTAGTTTAAATTTGTCTGAATTAGAACTTGGTTTCAAAGGGTGTTTGAATCTTAATCCAGAACAATTACCATCCACAACAGTAACACATTTTAGTGAGTTCAATTCTATGCAAACCCTTCAAAAAGATAGAATTGATCATTGTAGTAATATTCATGATGATGCTGTGAATGTGAATTTTGAGCTACCACCTTTACCAGGTGAAGAGGATTCATCATCACAAGATCATGTATTGTGGTCCAATTCTGAAATGATGATTAACCTTCCATTTCAAGTCACTCAACCTCCACCACCATTTCTTGGTCCTGATCAAATTCATGATGCAGACTTGTTAATCGGTAATTGGAGCCCTTTTGATTTTCCAAGGGATGCTTAA
Protein sequence

>MsG0180003247.01.T01

MESEWKANQISPNCPRCGSSNTKFCYYNNYSLTQPRYFCKGCRRYWTKGGSLRNVPVGGGCRKNRRGNNKTLVRHRSFDSLTFKNSPNSHTSLDNNNSISHHSYDPILRTNISSSSSSLVNDRPNIDLALVYANFLNQKPDSLSSDQMRKTNFDPCLENSRLSIGCLNLNPEQLPSTTVTHFSEFNSMQTLQKDRIDHCSNIHDDAVNVNFELPPLPGEEDSSSQDHVLWSNSEMMINLPFQVTQPPPPFLGPDQIHDADLLIGNWSPFDFPRDA*

>MsG0180003247.01.T02

MESEWKANQISPNCPRCGSSNTKFCYYNNYSLTQPRYFCKGCRRYWTKGGSLRNVPVGGGCRKNRRGNNKTLVRHRSFDSLTFKNSPNSHTSLDNNNSISHHSYDPILRTNISSSSSSLVNDRPNIDLALVYANFLNQKPDSLSSDQMRKTNFDPCLENSRLSIVEGGTSSLNLSELELGFKGCLNLNPEQLPSTTVTHFSEFNSMQTLQKDRIDHCSNIHDDAVNVNFELPPLPGEEDSSSQDHVLWSNSEMMINLPFQVTQPPPPFLGPDQIHDADLLIGNWSPFDFPRDA*