AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003306.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003306.01.T01 MTR_0907s0020 85.165 182 15 1 1 170 1 182 1.09e-97 280
MsG0180003306.01.T01 MTR_7g105790 56.021 191 59 4 1 169 1 188 5.70e-60 185
MsG0180003306.01.T01 MTR_4g052040 62.319 69 26 0 92 160 101 169 4.96e-25 95.5
MsG0180003306.01.T01 MTR_5g079710 42.222 135 66 3 24 158 37 159 3.31e-23 92.8
MsG0180003306.01.T01 MTR_4g052060 60.000 65 26 0 92 156 95 159 2.43e-21 86.3
MsG0180003306.01.T01 MTR_8g103520 52.778 72 33 1 85 156 220 290 1.18e-18 81.3
MsG0180003306.01.T01 MTR_5g075210 51.613 62 30 0 90 151 280 341 2.35e-16 75.5
MsG0180003306.01.T01 MTR_4g028930 44.928 69 38 0 83 151 307 375 1.34e-15 73.6
MsG0180003306.01.T01 MTR_7g072875 43.478 69 39 0 83 151 212 280 2.41e-14 69.3
MsG0180003306.01.T01 MTR_7g072630 43.478 69 39 0 83 151 274 342 4.22e-14 69.3
MsG0180003306.01.T01 MTR_3g111780 54.545 66 30 0 90 155 198 263 1.58e-13 67.0
MsG0180003306.01.T01 MTR_8g087530 48.438 64 33 0 88 151 256 319 1.90e-13 67.4
MsG0180003306.01.T01 MTR_2g018030 38.961 77 43 1 93 165 280 356 1.14e-12 65.1
MsG0180003306.01.T01 MTR_4g132740 42.424 66 38 0 92 157 83 148 6.56e-12 60.5
MsG0180003306.01.T02 MTR_0907s0020 83.516 182 11 2 1 163 1 182 1.25e-96 277
MsG0180003306.01.T02 MTR_7g105790 55.026 189 57 3 1 162 1 188 3.80e-59 183
MsG0180003306.01.T02 MTR_4g052040 62.319 69 26 0 85 153 101 169 7.53e-25 94.7
MsG0180003306.01.T02 MTR_5g079710 40.625 128 71 1 24 151 37 159 6.65e-24 94.4
MsG0180003306.01.T02 MTR_4g052060 36.364 165 83 3 1 149 1 159 1.09e-21 86.7
MsG0180003306.01.T02 MTR_8g103520 52.778 72 33 1 78 149 220 290 1.15e-18 80.9
MsG0180003306.01.T02 MTR_5g075210 51.613 62 30 0 83 144 280 341 2.44e-16 75.1
MsG0180003306.01.T02 MTR_4g028930 44.928 69 38 0 76 144 307 375 1.49e-15 73.2
MsG0180003306.01.T02 MTR_7g072875 43.478 69 39 0 76 144 212 280 2.48e-14 69.3
MsG0180003306.01.T02 MTR_7g072630 43.478 69 39 0 76 144 274 342 4.15e-14 68.9
MsG0180003306.01.T02 MTR_3g111780 54.545 66 30 0 83 148 198 263 1.73e-13 66.6
MsG0180003306.01.T02 MTR_8g087530 48.438 64 33 0 81 144 256 319 2.01e-13 67.0
MsG0180003306.01.T02 MTR_2g018030 38.961 77 43 1 86 158 280 356 1.31e-12 64.7
MsG0180003306.01.T02 MTR_4g132740 42.424 66 38 0 85 150 83 148 6.09e-12 60.5
MsG0180003306.01.T03 MTR_0907s0020 85.165 182 8 2 1 163 1 182 4.77e-97 278
MsG0180003306.01.T03 MTR_7g105790 55.026 189 57 3 1 162 1 188 1.16e-59 184
MsG0180003306.01.T03 MTR_4g052040 62.319 69 26 0 85 153 101 169 6.48e-25 95.1
MsG0180003306.01.T03 MTR_5g079710 44.961 129 64 3 24 151 37 159 9.63e-25 96.7
MsG0180003306.01.T03 MTR_4g052060 60.000 65 26 0 85 149 95 159 3.07e-21 85.5
MsG0180003306.01.T03 MTR_8g103520 52.778 72 33 1 78 149 220 290 1.23e-18 80.9
MsG0180003306.01.T03 MTR_5g075210 51.613 62 30 0 83 144 280 341 2.16e-16 75.5
MsG0180003306.01.T03 MTR_4g028930 44.928 69 38 0 76 144 307 375 1.31e-15 73.6
MsG0180003306.01.T03 MTR_7g072875 43.478 69 39 0 76 144 212 280 2.27e-14 69.3
MsG0180003306.01.T03 MTR_7g072630 43.478 69 39 0 76 144 274 342 3.58e-14 69.3
MsG0180003306.01.T03 MTR_3g111780 54.545 66 30 0 83 148 198 263 1.84e-13 66.6
MsG0180003306.01.T03 MTR_8g087530 48.438 64 33 0 81 144 256 319 1.99e-13 67.0
MsG0180003306.01.T03 MTR_2g018030 38.961 77 43 1 86 158 280 356 1.20e-12 64.7
MsG0180003306.01.T03 MTR_4g132740 42.424 66 38 0 85 150 83 148 4.79e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003306.01.T01 AT3G52525 45.355 183 63 4 1 174 1 155 8.24e-41 135
MsG0180003306.01.T01 AT2G36026 49.669 151 67 2 8 157 11 153 9.18e-36 123
MsG0180003306.01.T01 AT2G18500 52.941 68 32 0 87 154 223 290 2.92e-20 85.9
MsG0180003306.01.T01 AT5G19650 50.746 67 33 0 90 156 154 220 7.04e-20 83.2
MsG0180003306.01.T01 AT1G06920 32.824 131 78 2 33 163 194 314 6.06e-16 74.3
MsG0180003306.01.T01 AT5G58360 36.429 140 70 5 12 151 165 285 2.35e-15 72.4
MsG0180003306.01.T01 AT2G30400 51.613 62 30 0 90 151 252 313 3.31e-15 72.0
MsG0180003306.01.T01 AT5G01840 53.448 58 27 0 94 151 202 259 3.26e-14 68.9
MsG0180003306.01.T01 AT4G18830 47.059 68 36 0 85 152 277 344 1.05e-13 68.2
MsG0180003306.01.T01 AT5G22240 42.188 64 37 0 89 152 95 158 1.73e-12 63.2
MsG0180003306.01.T02 AT3G52525 47.727 176 62 5 1 167 1 155 1.57e-42 139
MsG0180003306.01.T02 AT2G36026 50.000 144 70 2 8 150 11 153 3.04e-36 124
MsG0180003306.01.T02 AT2G18500 52.941 68 32 0 80 147 223 290 3.76e-20 85.5
MsG0180003306.01.T02 AT5G19650 50.746 67 33 0 83 149 154 220 7.18e-20 83.2
MsG0180003306.01.T02 AT1G06920 38.318 107 56 2 50 156 218 314 2.97e-16 74.7
MsG0180003306.01.T02 AT2G30400 51.613 62 30 0 83 144 252 313 3.97e-15 71.6
MsG0180003306.01.T02 AT5G58360 34.586 133 75 3 12 144 165 285 5.19e-15 71.2
MsG0180003306.01.T02 AT5G01840 53.448 58 27 0 87 144 202 259 3.92e-14 68.6
MsG0180003306.01.T02 AT4G18830 47.059 68 36 0 78 145 277 344 1.18e-13 67.8
MsG0180003306.01.T02 AT5G22240 42.188 64 37 0 82 145 95 158 1.64e-12 63.2
MsG0180003306.01.T03 AT3G52525 47.727 176 62 5 1 167 1 155 1.59e-42 139
MsG0180003306.01.T03 AT2G36026 50.000 144 70 2 8 150 11 153 1.79e-36 124
MsG0180003306.01.T03 AT2G18500 52.941 68 32 0 80 147 223 290 4.21e-20 85.1
MsG0180003306.01.T03 AT5G19650 50.746 67 33 0 83 149 154 220 8.58e-20 82.8
MsG0180003306.01.T03 AT1G06920 37.383 107 57 2 50 156 218 314 2.29e-16 75.1
MsG0180003306.01.T03 AT5G58360 36.296 135 70 5 12 144 165 285 3.47e-15 71.6
MsG0180003306.01.T03 AT2G30400 51.613 62 30 0 83 144 252 313 3.85e-15 71.6
MsG0180003306.01.T03 AT5G01840 53.448 58 27 0 87 144 202 259 3.51e-14 68.6
MsG0180003306.01.T03 AT4G18830 47.059 68 36 0 78 145 277 344 8.89e-14 68.2
MsG0180003306.01.T03 AT5G22240 42.188 64 37 0 82 145 95 158 1.42e-12 63.2

Find 23 sgRNAs with CRISPR-Local

Find 38 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCCACAACCAAGGTTTA+TGG 0.203450 1:-60307977 None:intergenic
GTTTATGGAGACAGTGTTTA+AGG 0.204090 1:-60307962 None:intergenic
CCGTAACTCACGTGACTTCT+AGG 0.263433 1:+60308470 MsG0180003306.01.T03:CDS
TTGAACTAGAATTGTTATTA+AGG 0.278906 1:-60308083 None:intergenic
AAATCATCTTTGAAAGGATT+TGG 0.279931 1:+60308198 MsG0180003306.01.T03:CDS
TTCAATGTTGCAGATGATAT+TGG 0.300853 1:+60308287 MsG0180003306.01.T03:CDS
TTGAATATAAAACCAAGTTT+TGG 0.357213 1:-60308009 None:intergenic
TTGAAAGGATTTGGAAGAGT+TGG 0.393509 1:+60308207 MsG0180003306.01.T03:CDS
CCGAATGATGTGTTTGTTCT+CGG 0.398611 1:-60308168 None:intergenic
TTTGGAAGAGTTGGAAATGA+AGG 0.455515 1:+60308216 MsG0180003306.01.T03:CDS
CCGAGAACAAACACATCATT+CGG 0.478560 1:+60308168 MsG0180003306.01.T03:CDS
GAATGTCGAAAATCAAGATA+AGG 0.484941 1:-60308267 None:intergenic
AATGTCGAAAATCAAGATAA+GGG 0.494536 1:-60308266 None:intergenic
CCTAGAAGTCACGTGAGTTA+CGG 0.521811 1:-60308470 None:intergenic
AACACTGTCTCCATAAACCT+TGG 0.532041 1:+60307967 MsG0180003306.01.T03:CDS
CTGAATTCGAAGGAACATCA+TGG 0.538840 1:+60308366 MsG0180003306.01.T03:CDS
TCAGAGAAATCATCTTTGAA+AGG 0.539145 1:+60308192 MsG0180003306.01.T03:CDS
GTTTGTTCTCGGTGTTGTGT+TGG 0.550067 1:-60308157 None:intergenic
ACATGTACAAGACCAAAACT+TGG 0.559031 1:+60307997 MsG0180003306.01.T03:CDS
GTCTCCATAAACCTTGGTTG+TGG 0.580131 1:+60307973 MsG0180003306.01.T03:CDS
TGAATTCGAAGGAACATCAT+GGG 0.610258 1:+60308367 MsG0180003306.01.T03:CDS
TGGAAATGAAGGTGTAGCTG+TGG 0.614010 1:+60308227 MsG0180003306.01.T03:CDS
TTGTACATGTTCCACAACCA+AGG 0.618640 1:-60307984 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGAACTAGAATTGTTATTA+AGG - Chr1:60308086-60308105 None:intergenic 20.0%
!!! TTGAATATAAAACCAAGTTT+TGG - Chr1:60308012-60308031 None:intergenic 20.0%
! AATGTCGAAAATCAAGATAA+GGG - Chr1:60308269-60308288 None:intergenic 25.0%
!! AAATCATCTTTGAAAGGATT+TGG + Chr1:60308198-60308217 MsG0180003306.01.T03:CDS 25.0%
!!! GTTTAAGGTTAGTTTTTTTC+TGG - Chr1:60307950-60307969 None:intergenic 25.0%
!!! TATTAAGGTGATGTTTTTTG+TGG - Chr1:60308071-60308090 None:intergenic 25.0%
GAATGTCGAAAATCAAGATA+AGG - Chr1:60308270-60308289 None:intergenic 30.0%
TCAGAGAAATCATCTTTGAA+AGG + Chr1:60308192-60308211 MsG0180003306.01.T03:CDS 30.0%
TTCAATGTTGCAGATGATAT+TGG + Chr1:60308287-60308306 MsG0180003306.01.T03:CDS 30.0%
! TTAGAGCTTTTACTGAGATT+TGG + Chr1:60308397-60308416 MsG0180003306.01.T03:CDS 30.0%
!! ACAATTCTAGTTCAACCATT+TGG + Chr1:60308091-60308110 MsG0180003306.01.T03:intron 30.0%
!!! TAAGGTTAGTTTTTTTCTGG+AGG - Chr1:60307947-60307966 None:intergenic 30.0%
!!! TTAAGTCTGGTTTTTCATCT+GGG + Chr1:60308436-60308455 MsG0180003306.01.T03:CDS 30.0%
!!! TTTTTGTGGTTTTGGTAAGT+GGG - Chr1:60308057-60308076 None:intergenic 30.0%
!!! TTTTTTGTGGTTTTGGTAAG+TGG - Chr1:60308058-60308077 None:intergenic 30.0%
ACATGTACAAGACCAAAACT+TGG + Chr1:60307997-60308016 MsG0180003306.01.T03:CDS 35.0%
GTTTATGGAGACAGTGTTTA+AGG - Chr1:60307965-60307984 None:intergenic 35.0%
TCTGTCTTTCTGTTTCCAAA+TGG - Chr1:60308109-60308128 None:intergenic 35.0%
TGAATTCGAAGGAACATCAT+GGG + Chr1:60308367-60308386 MsG0180003306.01.T03:CDS 35.0%
TTGAAAGGATTTGGAAGAGT+TGG + Chr1:60308207-60308226 MsG0180003306.01.T03:CDS 35.0%
TTTGGAAGAGTTGGAAATGA+AGG + Chr1:60308216-60308235 MsG0180003306.01.T03:CDS 35.0%
! GCTTTTACTGAGATTTGGAA+TGG + Chr1:60308402-60308421 MsG0180003306.01.T03:CDS 35.0%
! TAAGTGGGGTTTTTGTGTTT+TGG - Chr1:60308042-60308061 None:intergenic 35.0%
! TTTTCTTCAGCTGAATTCGA+AGG + Chr1:60308356-60308375 MsG0180003306.01.T03:CDS 35.0%
!!! GGTGATGTTTTTTGTGGTTT+TGG - Chr1:60308065-60308084 None:intergenic 35.0%
!!! GTTAAGTCTGGTTTTTCATC+TGG + Chr1:60308435-60308454 MsG0180003306.01.T03:CDS 35.0%
!!! TTTTGTGGTTTTGGTAAGTG+GGG - Chr1:60308056-60308075 None:intergenic 35.0%
AACACTGTCTCCATAAACCT+TGG + Chr1:60307967-60307986 MsG0180003306.01.T03:CDS 40.0%
CCGAATGATGTGTTTGTTCT+CGG - Chr1:60308171-60308190 None:intergenic 40.0%
CCGAGAACAAACACATCATT+CGG + Chr1:60308168-60308187 MsG0180003306.01.T03:CDS 40.0%
CTGAATTCGAAGGAACATCA+TGG + Chr1:60308366-60308385 MsG0180003306.01.T03:CDS 40.0%
TGTTCCACAACCAAGGTTTA+TGG - Chr1:60307980-60307999 None:intergenic 40.0%
TTGTACATGTTCCACAACCA+AGG - Chr1:60307987-60308006 None:intergenic 40.0%
! GGAGTTTTCTCTGTTAAGTC+TGG + Chr1:60308423-60308442 MsG0180003306.01.T03:CDS 40.0%
GTCTCCATAAACCTTGGTTG+TGG + Chr1:60307973-60307992 MsG0180003306.01.T03:CDS 45.0%
! TGGAAATGAAGGTGTAGCTG+TGG + Chr1:60308227-60308246 MsG0180003306.01.T03:CDS 45.0%
!! GTTTGTTCTCGGTGTTGTGT+TGG - Chr1:60308160-60308179 None:intergenic 45.0%
!! CGGTGTTGTGTTGGTTGTGC+TGG - Chr1:60308151-60308170 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 60307937 60308491 60307937 ID=MsG0180003306.01;Name=MsG0180003306.01
Chr1 mRNA 60307937 60308491 60307937 ID=MsG0180003306.01.T01;Parent=MsG0180003306.01;Name=MsG0180003306.01.T01;_AED=0.03;_eAED=0.03;_QI=0|0|0|1|0|0|2|0|174
Chr1 exon 60307937 60308108 60307937 ID=MsG0180003306.01.T01:exon:49327;Parent=MsG0180003306.01.T01
Chr1 CDS 60307937 60308108 60307937 ID=MsG0180003306.01.T01:cds;Parent=MsG0180003306.01.T01
Chr1 CDS 60308139 60308491 60308139 ID=MsG0180003306.01.T01:cds;Parent=MsG0180003306.01.T01
Chr1 mRNA 60307937 60308491 60307937 ID=MsG0180003306.01.T02;Parent=MsG0180003306.01;Name=MsG0180003306.01.T02;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|0|0|2|0|167
Chr1 exon 60307937 60308100 60307937 ID=MsG0180003306.01.T02:exon:49329;Parent=MsG0180003306.01.T02
Chr1 exon 60308152 60308491 60308152 ID=MsG0180003306.01.T02:exon:49330;Parent=MsG0180003306.01.T02
Chr1 CDS 60307937 60308100 60307937 ID=MsG0180003306.01.T02:cds;Parent=MsG0180003306.01.T02
Chr1 CDS 60308152 60308491 60308152 ID=MsG0180003306.01.T02:cds;Parent=MsG0180003306.01.T02
Chr1 mRNA 60307937 60308491 60307937 ID=MsG0180003306.01.T03;Parent=MsG0180003306.01;Name=MsG0180003306.01.T03;_AED=0.05;_eAED=0.05;_QI=0|0|0|1|0|0|2|0|167
Chr1 exon 60307937 60308087 60307937 ID=MsG0180003306.01.T03:exon:49331;Parent=MsG0180003306.01.T03
Chr1 CDS 60307937 60308087 60307937 ID=MsG0180003306.01.T03:cds;Parent=MsG0180003306.01.T03
Chr1 CDS 60308139 60308491 60308139 ID=MsG0180003306.01.T03:cds;Parent=MsG0180003306.01.T03
Gene Sequence

>MsG0180003306.01.T01

ATGTCTACCTCCAGAAAAAAACTAACCTTAAACACTGTCTCCATAAACCTTGGTTGTGGAACATGTACAAGACCAAAACTTGGTTTTATATTCAACACAAAACCAAAACACAAAAACCCCACTTACCAAAACCACAAAAAACATCACCTTAATAACAATTCTAGTTCAACCAAAACTTCAACCAGCACAACCAACACAACACCGAGAACAAACACATCATTCGGCTCAGAGAAATCATCTTTGAAAGGATTTGGAAGAGTTGGAAATGAAGGTGTAGCTGTGGAGAAAGATTCTGATGACCCTTATCTTGATTTTCGACATTCAATGTTGCAGATGATATTGGAGAATGAGATATATTCTAAAGATGATCTTAGAGAGCTTTTGAACTGTTTTCTTCAGCTGAATTCGAAGGAACATCATGGGATTATATTTAGAGCTTTTACTGAGATTTGGAATGGAGTTTTCTCTGTTAAGTCTGGTTTTTCATCTGGGTTTCACGTTAACCGTAACTCACGTGACTTCTAG

>MsG0180003306.01.T02

ATGTCTACCTCCAGAAAAAAACTAACCTTAAACACTGTCTCCATAAACCTTGGTTGTGGAACATGTACAAGACCAAAACTTGGTTTTATATTCAACACAAAACCAAAACACAAAAACCCCACTTACCAAAACCACAAAAAACATCACCTTAATAACAATTCTAGCACAACCAACACAACACCGAGAACAAACACATCATTCGGCTCAGAGAAATCATCTTTGAAAGGATTTGGAAGAGTTGGAAATGAAGGTGTAGCTGTGGAGAAAGATTCTGATGACCCTTATCTTGATTTTCGACATTCAATGTTGCAGATGATATTGGAGAATGAGATATATTCTAAAGATGATCTTAGAGAGCTTTTGAACTGTTTTCTTCAGCTGAATTCGAAGGAACATCATGGGATTATATTTAGAGCTTTTACTGAGATTTGGAATGGAGTTTTCTCTGTTAAGTCTGGTTTTTCATCTGGGTTTCACGTTAACCGTAACTCACGTGACTTCTAG

>MsG0180003306.01.T03

ATGTCTACCTCCAGAAAAAAACTAACCTTAAACACTGTCTCCATAAACCTTGGTTGTGGAACATGTACAAGACCAAAACTTGGTTTTATATTCAACACAAAACCAAAACACAAAAACCCCACTTACCAAAACCACAAAAAACATCACCTTAAAACTTCAACCAGCACAACCAACACAACACCGAGAACAAACACATCATTCGGCTCAGAGAAATCATCTTTGAAAGGATTTGGAAGAGTTGGAAATGAAGGTGTAGCTGTGGAGAAAGATTCTGATGACCCTTATCTTGATTTTCGACATTCAATGTTGCAGATGATATTGGAGAATGAGATATATTCTAAAGATGATCTTAGAGAGCTTTTGAACTGTTTTCTTCAGCTGAATTCGAAGGAACATCATGGGATTATATTTAGAGCTTTTACTGAGATTTGGAATGGAGTTTTCTCTGTTAAGTCTGGTTTTTCATCTGGGTTTCACGTTAACCGTAACTCACGTGACTTCTAG

Protein sequence

>MsG0180003306.01.T01

MSTSRKKLTLNTVSINLGCGTCTRPKLGFIFNTKPKHKNPTYQNHKKHHLNNNSSSTKTSTSTTNTTPRTNTSFGSEKSSLKGFGRVGNEGVAVEKDSDDPYLDFRHSMLQMILENEIYSKDDLRELLNCFLQLNSKEHHGIIFRAFTEIWNGVFSVKSGFSSGFHVNRNSRDF*

>MsG0180003306.01.T02

MSTSRKKLTLNTVSINLGCGTCTRPKLGFIFNTKPKHKNPTYQNHKKHHLNNNSSTTNTTPRTNTSFGSEKSSLKGFGRVGNEGVAVEKDSDDPYLDFRHSMLQMILENEIYSKDDLRELLNCFLQLNSKEHHGIIFRAFTEIWNGVFSVKSGFSSGFHVNRNSRDF*

>MsG0180003306.01.T03

MSTSRKKLTLNTVSINLGCGTCTRPKLGFIFNTKPKHKNPTYQNHKKHHLKTSTSTTNTTPRTNTSFGSEKSSLKGFGRVGNEGVAVEKDSDDPYLDFRHSMLQMILENEIYSKDDLRELLNCFLQLNSKEHHGIIFRAFTEIWNGVFSVKSGFSSGFHVNRNSRDF*