Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004214.01.T01 | XP_013468828.1 | 91.379 | 116 | 10 | 0 | 1 | 116 | 1 | 116 | 1.09E-61 | 196 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004214.01.T01 | Q7XJK6 | 45.528 | 123 | 65 | 2 | 4 | 125 | 2 | 123 | 6.56E-26 | 102 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004214.01.T01 | A0A072VXU8 | 91.379 | 116 | 10 | 0 | 1 | 116 | 1 | 116 | 5.20e-62 | 196 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180004214.01.T01 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004214.01.T01 | MTR_1g077300 | 91.379 | 116 | 10 | 0 | 1 | 116 | 1 | 116 | 1.32e-65 | 196 |
| MsG0180004214.01.T01 | MTR_1g077320 | 82.906 | 117 | 20 | 0 | 1 | 117 | 1 | 117 | 1.68e-61 | 186 |
| MsG0180004214.01.T01 | MTR_5g047580 | 79.464 | 112 | 23 | 0 | 1 | 112 | 1 | 112 | 5.79e-60 | 182 |
| MsG0180004214.01.T01 | MTR_4g028720 | 80.342 | 117 | 23 | 0 | 1 | 117 | 1 | 117 | 4.71e-58 | 177 |
| MsG0180004214.01.T01 | MTR_7g011950 | 81.579 | 114 | 21 | 0 | 1 | 114 | 1 | 114 | 2.23e-57 | 175 |
| MsG0180004214.01.T01 | MTR_1g077390 | 71.795 | 117 | 33 | 0 | 1 | 117 | 1 | 117 | 5.52e-56 | 171 |
| MsG0180004214.01.T01 | MTR_1g084950 | 75.410 | 122 | 30 | 0 | 1 | 122 | 1 | 122 | 2.84e-54 | 167 |
| MsG0180004214.01.T01 | MTR_1g090697 | 57.600 | 125 | 53 | 0 | 1 | 125 | 1 | 125 | 6.81e-48 | 151 |
| MsG0180004214.01.T01 | MTR_1g090783 | 58.824 | 119 | 48 | 1 | 2 | 120 | 1 | 118 | 2.72e-45 | 144 |
| MsG0180004214.01.T01 | MTR_1g090710 | 56.410 | 117 | 51 | 0 | 1 | 117 | 1 | 117 | 8.18e-45 | 143 |
| MsG0180004214.01.T01 | MTR_5g047560 | 64.286 | 112 | 24 | 1 | 1 | 112 | 1 | 96 | 2.59e-42 | 136 |
| MsG0180004214.01.T01 | MTR_3g466980 | 45.669 | 127 | 67 | 2 | 1 | 126 | 1 | 126 | 3.29e-30 | 108 |
| MsG0180004214.01.T01 | MTR_4g032620 | 43.478 | 115 | 64 | 1 | 1 | 114 | 1 | 115 | 5.67e-30 | 107 |
| MsG0180004214.01.T01 | MTR_3g466830 | 49.550 | 111 | 55 | 1 | 2 | 111 | 1 | 111 | 9.10e-30 | 105 |
| MsG0180004214.01.T01 | MTR_3g031240 | 46.018 | 113 | 60 | 1 | 1 | 112 | 1 | 113 | 2.45e-29 | 105 |
| MsG0180004214.01.T01 | MTR_3g065100 | 54.867 | 113 | 50 | 1 | 1 | 112 | 1 | 113 | 1.05e-28 | 105 |
| MsG0180004214.01.T01 | MTR_4g063790 | 46.903 | 113 | 59 | 1 | 1 | 112 | 1 | 113 | 4.99e-28 | 101 |
| MsG0180004214.01.T01 | MTR_4g031910 | 48.760 | 121 | 60 | 2 | 1 | 120 | 1 | 120 | 5.21e-27 | 100 |
| MsG0180004214.01.T01 | MTR_8g036130 | 46.535 | 101 | 51 | 1 | 1 | 98 | 1 | 101 | 1.64e-26 | 99.4 |
| MsG0180004214.01.T01 | MTR_2g035610 | 43.802 | 121 | 66 | 2 | 1 | 120 | 1 | 120 | 7.46e-26 | 94.4 |
| MsG0180004214.01.T01 | MTR_3g466890 | 44.144 | 111 | 61 | 1 | 2 | 111 | 1 | 111 | 3.50e-25 | 93.6 |
| MsG0180004214.01.T01 | MTR_3g467080 | 44.248 | 113 | 62 | 1 | 1 | 112 | 1 | 113 | 4.34e-25 | 93.6 |
| MsG0180004214.01.T01 | MTR_5g075380 | 37.607 | 117 | 72 | 1 | 1 | 116 | 1 | 117 | 6.03e-24 | 90.5 |
| MsG0180004214.01.T01 | MTR_4g032290 | 47.107 | 121 | 62 | 2 | 1 | 120 | 1 | 120 | 3.04e-23 | 90.5 |
| MsG0180004214.01.T01 | MTR_3g031100 | 40.708 | 113 | 66 | 1 | 1 | 112 | 1 | 113 | 5.65e-23 | 90.1 |
| MsG0180004214.01.T01 | MTR_2g016210 | 39.200 | 125 | 75 | 1 | 1 | 124 | 1 | 125 | 6.90e-22 | 87.4 |
| MsG0180004214.01.T01 | MTR_4g032260 | 44.262 | 122 | 64 | 3 | 1 | 120 | 1 | 120 | 1.91e-21 | 84.3 |
| MsG0180004214.01.T01 | MTR_4g028800 | 50.000 | 74 | 35 | 1 | 1 | 74 | 1 | 72 | 4.01e-21 | 80.9 |
| MsG0180004214.01.T01 | MTR_4g019670 | 37.037 | 108 | 67 | 1 | 3 | 109 | 6 | 113 | 1.98e-20 | 85.9 |
| MsG0180004214.01.T01 | MTR_7g106510 | 36.111 | 108 | 68 | 1 | 3 | 109 | 6 | 113 | 4.16e-20 | 84.7 |
| MsG0180004214.01.T01 | MTR_3g093900 | 32.479 | 117 | 74 | 2 | 3 | 116 | 6 | 120 | 1.41e-16 | 74.7 |
| MsG0180004214.01.T01 | MTR_2g035580 | 37.234 | 94 | 58 | 1 | 1 | 93 | 1 | 94 | 2.70e-16 | 70.9 |
| MsG0180004214.01.T01 | MTR_3g466930 | 43.210 | 81 | 45 | 1 | 32 | 111 | 2 | 82 | 3.68e-16 | 69.7 |
| MsG0180004214.01.T01 | MTR_3g466900 | 41.975 | 81 | 46 | 1 | 32 | 111 | 2 | 82 | 7.73e-16 | 68.9 |
| MsG0180004214.01.T01 | MTR_3g465410 | 42.353 | 85 | 48 | 1 | 29 | 112 | 1 | 85 | 1.41e-15 | 68.6 |
| MsG0180004214.01.T01 | MTR_7g055800 | 36.522 | 115 | 44 | 3 | 1 | 114 | 1 | 87 | 2.12e-15 | 66.6 |
| MsG0180004214.01.T01 | MTR_7g055790 | 39.640 | 111 | 48 | 2 | 1 | 110 | 59 | 151 | 1.09e-14 | 66.6 |
| MsG0180004214.01.T01 | MTR_7g055940 | 35.922 | 103 | 37 | 4 | 1 | 102 | 1 | 75 | 7.86e-11 | 54.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004214.01.T01 | AT3G05860 | 46.491 | 114 | 60 | 1 | 1 | 113 | 1 | 114 | 7.98e-31 | 109 |
| MsG0180004214.01.T01 | AT3G05860 | 46.491 | 114 | 60 | 1 | 1 | 113 | 1 | 114 | 3.01e-30 | 109 |
| MsG0180004214.01.T01 | AT3G05860 | 46.491 | 114 | 60 | 1 | 1 | 113 | 1 | 114 | 3.70e-30 | 109 |
| MsG0180004214.01.T01 | AT5G26630 | 50.413 | 121 | 58 | 2 | 1 | 120 | 1 | 120 | 1.63e-28 | 103 |
| MsG0180004214.01.T01 | AT1G65330 | 45.133 | 113 | 61 | 1 | 2 | 113 | 1 | 113 | 3.90e-27 | 101 |
| MsG0180004214.01.T01 | AT5G26650 | 45.528 | 123 | 65 | 2 | 4 | 125 | 2 | 123 | 6.69e-27 | 102 |
| MsG0180004214.01.T01 | AT5G48670 | 49.206 | 126 | 62 | 2 | 1 | 125 | 1 | 125 | 6.99e-27 | 102 |
| MsG0180004214.01.T01 | AT1G65300 | 40.833 | 120 | 69 | 2 | 2 | 120 | 1 | 119 | 2.75e-26 | 99.4 |
| MsG0180004214.01.T01 | AT5G27960 | 43.902 | 123 | 67 | 2 | 4 | 125 | 2 | 123 | 7.50e-26 | 99.4 |
| MsG0180004214.01.T01 | AT1G31640 | 41.525 | 118 | 67 | 2 | 2 | 118 | 1 | 117 | 4.86e-24 | 95.9 |
| MsG0180004214.01.T01 | AT1G31630 | 37.288 | 118 | 72 | 2 | 2 | 118 | 1 | 117 | 2.62e-23 | 92.8 |
| MsG0180004214.01.T01 | AT1G22590 | 38.843 | 121 | 61 | 2 | 1 | 114 | 1 | 115 | 9.88e-22 | 85.1 |
| MsG0180004214.01.T01 | AT2G28700 | 37.302 | 126 | 77 | 2 | 1 | 125 | 1 | 125 | 3.96e-21 | 86.7 |
| MsG0180004214.01.T01 | AT5G27810 | 43.529 | 85 | 47 | 1 | 29 | 112 | 1 | 85 | 4.78e-19 | 77.0 |
| MsG0180004214.01.T01 | AT5G26580 | 38.400 | 125 | 63 | 2 | 1 | 125 | 1 | 111 | 6.90e-19 | 80.9 |
| MsG0180004214.01.T01 | AT2G40210 | 37.864 | 103 | 59 | 2 | 1 | 100 | 1 | 101 | 9.59e-12 | 60.8 |
Find 22 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGCAAGAGAAAAGTTTCT+TGG | 0.265401 | 1:+74940911 | None:intergenic |
| CTCCTTTCTTCTTGAAGCAT+TGG | 0.353516 | 1:+74941128 | None:intergenic |
| GGAGCTAAAGATTCGTATAA+TGG | 0.378777 | 1:-74940833 | MsG0180004214.01.T01:CDS |
| AAATCTGAAGGAAGCAAAAC+AGG | 0.418438 | 1:-74940986 | MsG0180004214.01.T01:CDS |
| TCTGAAACAGTCAAATCATC+TGG | 0.455315 | 1:+74940794 | None:intergenic |
| CTCCAATGCTTCAAGAAGAA+AGG | 0.468651 | 1:-74941130 | MsG0180004214.01.T01:CDS |
| CAAGGCCATGGTTCGGAAGA+AGG | 0.471800 | 1:-74941169 | None:intergenic |
| TACATTGCAAGGCCATGGTT+CGG | 0.507534 | 1:-74941176 | None:intergenic |
| GACATACAGAAAGAGAAAGA+AGG | 0.512113 | 1:-74941106 | MsG0180004214.01.T01:CDS |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 1:-74941066 | MsG0180004214.01.T01:CDS |
| ACATACAGAAAGAGAAAGAA+GGG | 0.541098 | 1:-74941105 | MsG0180004214.01.T01:CDS |
| TGCAGCAGATTACTAAAGCT+AGG | 0.546331 | 1:-74940894 | MsG0180004214.01.T01:CDS |
| AAACAGTCAAATCATCTGGC+AGG | 0.548613 | 1:+74940798 | None:intergenic |
| AGGATCGGAAATTATAGCAC+AGG | 0.559456 | 1:+74941035 | None:intergenic |
| AGAGGTGTGGCCAAATCTGA+AGG | 0.570010 | 1:-74940998 | MsG0180004214.01.T01:CDS |
| TCGGAAATTATAGCACAGGC+TGG | 0.570119 | 1:+74941039 | None:intergenic |
| AAAGAAGGGTATCATCAAGA+AGG | 0.577075 | 1:-74941091 | MsG0180004214.01.T01:CDS |
| CTTCACCTTCTTCCGAACCA+TGG | 0.581108 | 1:+74941164 | None:intergenic |
| GCAGCAGATTACTAAAGCTA+GGG | 0.592338 | 1:-74940893 | MsG0180004214.01.T01:CDS |
| AAGCAAAACAGGTGATTGAG+AGG | 0.611042 | 1:-74940975 | MsG0180004214.01.T01:CDS |
| GAAACAAGACAATCGTGAGA+AGG | 0.628912 | 1:-74940854 | MsG0180004214.01.T01:CDS |
| TTGATTCTAAAACAGAGGTG+TGG | 0.633348 | 1:-74941011 | MsG0180004214.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGTTTTAGAATCAAAAGGAT+CGG | + | Chr1:74940949-74940968 | None:intergenic | 25.0% |
| ACATACAGAAAGAGAAAGAA+GGG | - | Chr1:74940861-74940880 | MsG0180004214.01.T01:CDS | 30.0% | |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | + | Chr1:74940954-74940973 | None:intergenic | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | - | Chr1:74940950-74940969 | MsG0180004214.01.T01:CDS | 30.0% |
| AAATCTGAAGGAAGCAAAAC+AGG | - | Chr1:74940980-74940999 | MsG0180004214.01.T01:CDS | 35.0% | |
| AGAGAACTCACTATTCTTTG+TGG | - | Chr1:74940900-74940919 | MsG0180004214.01.T01:CDS | 35.0% | |
| GACATACAGAAAGAGAAAGA+AGG | - | Chr1:74940860-74940879 | MsG0180004214.01.T01:CDS | 35.0% | |
| GGAGCTAAAGATTCGTATAA+TGG | - | Chr1:74941133-74941152 | MsG0180004214.01.T01:CDS | 35.0% | |
| ! | TGTTTTGCTTCCTTCAGATT+TGG | + | Chr1:74940981-74941000 | None:intergenic | 35.0% |
| !! | AAAGAAGGGTATCATCAAGA+AGG | - | Chr1:74940875-74940894 | MsG0180004214.01.T01:CDS | 35.0% |
| !! | TTGATTCTAAAACAGAGGTG+TGG | - | Chr1:74940955-74940974 | MsG0180004214.01.T01:CDS | 35.0% |
| AAGCAAAACAGGTGATTGAG+AGG | - | Chr1:74940991-74941010 | MsG0180004214.01.T01:CDS | 40.0% | |
| AGGATCGGAAATTATAGCAC+AGG | + | Chr1:74940934-74940953 | None:intergenic | 40.0% | |
| CTCCAATGCTTCAAGAAGAA+AGG | - | Chr1:74940836-74940855 | MsG0180004214.01.T01:CDS | 40.0% | |
| CTCCTTTCTTCTTGAAGCAT+TGG | + | Chr1:74940841-74940860 | None:intergenic | 40.0% | |
| GAAACAAGACAATCGTGAGA+AGG | - | Chr1:74941112-74941131 | MsG0180004214.01.T01:CDS | 40.0% | |
| GCAGCAGATTACTAAAGCTA+GGG | - | Chr1:74941073-74941092 | MsG0180004214.01.T01:CDS | 40.0% | |
| GCTGCAAGAGAAAAGTTTCT+TGG | + | Chr1:74941058-74941077 | None:intergenic | 40.0% | |
| TGCAGCAGATTACTAAAGCT+AGG | - | Chr1:74941072-74941091 | MsG0180004214.01.T01:CDS | 40.0% | |
| TCGGAAATTATAGCACAGGC+TGG | + | Chr1:74940930-74940949 | None:intergenic | 45.0% | |
| ! | AGAGGTGTGGCCAAATCTGA+AGG | - | Chr1:74940968-74940987 | MsG0180004214.01.T01:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 74940804 | 74941184 | 74940804 | ID=MsG0180004214.01;Name=MsG0180004214.01 |
| Chr1 | mRNA | 74940804 | 74941184 | 74940804 | ID=MsG0180004214.01.T01;Parent=MsG0180004214.01;Name=MsG0180004214.01.T01;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|1|1|0|126 |
| Chr1 | exon | 74940804 | 74941184 | 74940804 | ID=MsG0180004214.01.T01:exon:5702;Parent=MsG0180004214.01.T01 |
| Chr1 | CDS | 74940804 | 74941184 | 74940804 | ID=MsG0180004214.01.T01:cds;Parent=MsG0180004214.01.T01 |
| Gene Sequence |
| Protein sequence |
