Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004236.01.T01 | XP_013468829.1 | 84.906 | 159 | 8 | 1 | 1 | 143 | 1 | 159 | 4.81E-85 | 256 |
| MsG0180004236.01.T03 | XP_013468829.1 | 95.238 | 84 | 4 | 0 | 1 | 84 | 76 | 159 | 1.30E-48 | 161 |
| MsG0180004236.01.T02 | XP_013468829.1 | 95.597 | 159 | 7 | 0 | 1 | 159 | 1 | 159 | 1.22E-98 | 291 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004236.01.T01 | Q9FJK3 | 38.462 | 156 | 79 | 2 | 1 | 139 | 1 | 156 | 4.29E-23 | 95.1 |
| MsG0180004236.01.T02 | Q9FJK3 | 45.098 | 153 | 83 | 1 | 1 | 152 | 1 | 153 | 1.11E-32 | 120 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004236.01.T01 | A0A072VMQ1 | 84.906 | 159 | 8 | 1 | 1 | 143 | 1 | 159 | 2.30e-85 | 256 |
| MsG0180004236.01.T02 | A0A072VMQ1 | 95.597 | 159 | 7 | 0 | 1 | 159 | 1 | 159 | 5.84e-99 | 291 |
| MsG0180004236.01.T03 | A0A072VMQ1 | 95.238 | 84 | 4 | 0 | 1 | 84 | 76 | 159 | 6.23e-49 | 161 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180004236.01.T02 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004236.01.T01 | MTR_1g077320 | 84.906 | 159 | 8 | 1 | 1 | 143 | 1 | 159 | 5.83e-89 | 256 |
| MsG0180004236.01.T01 | MTR_7g011950 | 81.761 | 159 | 13 | 1 | 1 | 143 | 1 | 159 | 1.66e-84 | 244 |
| MsG0180004236.01.T01 | MTR_4g028720 | 78.616 | 159 | 18 | 1 | 1 | 143 | 1 | 159 | 1.85e-82 | 239 |
| MsG0180004236.01.T01 | MTR_1g077390 | 62.420 | 157 | 43 | 1 | 1 | 141 | 1 | 157 | 1.43e-64 | 194 |
| MsG0180004236.01.T01 | MTR_1g084950 | 65.385 | 156 | 38 | 1 | 1 | 140 | 1 | 156 | 7.49e-64 | 192 |
| MsG0180004236.01.T01 | MTR_1g077300 | 66.038 | 159 | 36 | 2 | 1 | 143 | 1 | 157 | 1.49e-62 | 189 |
| MsG0180004236.01.T01 | MTR_5g047560 | 65.000 | 140 | 49 | 0 | 1 | 140 | 1 | 140 | 4.85e-62 | 187 |
| MsG0180004236.01.T01 | MTR_5g047580 | 59.615 | 156 | 47 | 1 | 1 | 140 | 1 | 156 | 1.52e-59 | 181 |
| MsG0180004236.01.T01 | MTR_1g090697 | 49.032 | 155 | 63 | 1 | 1 | 139 | 1 | 155 | 7.51e-47 | 149 |
| MsG0180004236.01.T01 | MTR_1g090710 | 48.718 | 156 | 63 | 2 | 1 | 139 | 1 | 156 | 5.78e-45 | 144 |
| MsG0180004236.01.T01 | MTR_1g090783 | 47.712 | 153 | 64 | 1 | 3 | 139 | 2 | 154 | 3.91e-43 | 140 |
| MsG0180004236.01.T01 | MTR_3g065100 | 43.312 | 157 | 72 | 2 | 1 | 140 | 1 | 157 | 4.38e-30 | 109 |
| MsG0180004236.01.T01 | MTR_3g466980 | 39.216 | 153 | 76 | 2 | 1 | 136 | 1 | 153 | 3.40e-27 | 101 |
| MsG0180004236.01.T01 | MTR_5g075380 | 33.974 | 156 | 86 | 2 | 1 | 139 | 1 | 156 | 5.91e-26 | 96.3 |
| MsG0180004236.01.T01 | MTR_4g032620 | 32.258 | 155 | 87 | 3 | 1 | 137 | 1 | 155 | 5.70e-25 | 95.5 |
| MsG0180004236.01.T01 | MTR_4g063790 | 35.220 | 159 | 85 | 3 | 1 | 141 | 1 | 159 | 2.97e-23 | 89.7 |
| MsG0180004236.01.T01 | MTR_4g031910 | 36.667 | 150 | 77 | 3 | 1 | 132 | 1 | 150 | 6.43e-23 | 90.1 |
| MsG0180004236.01.T01 | MTR_3g466890 | 34.416 | 154 | 84 | 2 | 3 | 139 | 2 | 155 | 2.62e-21 | 84.3 |
| MsG0180004236.01.T01 | MTR_4g032260 | 35.000 | 160 | 86 | 3 | 1 | 142 | 1 | 160 | 8.70e-21 | 83.2 |
| MsG0180004236.01.T01 | MTR_8g036130 | 33.333 | 159 | 86 | 3 | 1 | 139 | 1 | 159 | 1.40e-20 | 84.7 |
| MsG0180004236.01.T01 | MTR_3g466830 | 34.395 | 157 | 86 | 2 | 3 | 142 | 2 | 158 | 3.60e-20 | 81.6 |
| MsG0180004236.01.T01 | MTR_4g032290 | 32.667 | 150 | 83 | 3 | 1 | 132 | 1 | 150 | 5.96e-20 | 82.4 |
| MsG0180004236.01.T01 | MTR_2g016210 | 36.250 | 160 | 82 | 4 | 1 | 141 | 1 | 159 | 6.98e-19 | 80.1 |
| MsG0180004236.01.T01 | MTR_3g031100 | 31.013 | 158 | 89 | 4 | 1 | 139 | 1 | 157 | 7.13e-17 | 74.7 |
| MsG0180004236.01.T01 | MTR_2g035610 | 33.884 | 121 | 63 | 2 | 1 | 104 | 1 | 121 | 1.76e-16 | 70.9 |
| MsG0180004236.01.T01 | MTR_3g467080 | 30.000 | 160 | 92 | 4 | 1 | 141 | 1 | 159 | 2.12e-16 | 71.6 |
| MsG0180004236.01.T01 | MTR_3g031240 | 28.834 | 163 | 90 | 4 | 1 | 141 | 1 | 159 | 3.07e-16 | 72.0 |
| MsG0180004236.01.T01 | MTR_3g465410 | 33.898 | 118 | 76 | 2 | 26 | 141 | 14 | 131 | 4.88e-15 | 67.8 |
| MsG0180004236.01.T01 | MTR_1g033930 | 39.796 | 98 | 58 | 1 | 46 | 142 | 20 | 117 | 1.65e-14 | 65.9 |
| MsG0180004236.01.T01 | MTR_1g033370 | 43.011 | 93 | 52 | 1 | 48 | 139 | 28 | 120 | 1.80e-14 | 65.9 |
| MsG0180004236.01.T01 | MTR_3g466900 | 34.211 | 114 | 74 | 1 | 27 | 139 | 13 | 126 | 2.69e-14 | 65.5 |
| MsG0180004236.01.T01 | MTR_1g033790 | 40.816 | 98 | 57 | 1 | 46 | 142 | 20 | 117 | 6.08e-14 | 64.3 |
| MsG0180004236.01.T01 | MTR_3g466930 | 32.456 | 114 | 76 | 1 | 27 | 139 | 13 | 126 | 5.90e-13 | 62.0 |
| MsG0180004236.01.T03 | MTR_1g077320 | 95.238 | 84 | 4 | 0 | 1 | 84 | 76 | 159 | 1.58e-52 | 161 |
| MsG0180004236.01.T03 | MTR_7g011950 | 86.905 | 84 | 11 | 0 | 1 | 84 | 76 | 159 | 6.09e-47 | 147 |
| MsG0180004236.01.T03 | MTR_4g028720 | 86.905 | 84 | 11 | 0 | 1 | 84 | 76 | 159 | 8.18e-47 | 146 |
| MsG0180004236.01.T03 | MTR_1g077300 | 69.048 | 84 | 24 | 1 | 1 | 84 | 76 | 157 | 8.50e-33 | 111 |
| MsG0180004236.01.T03 | MTR_1g084950 | 65.432 | 81 | 28 | 0 | 1 | 81 | 76 | 156 | 6.35e-32 | 108 |
| MsG0180004236.01.T03 | MTR_1g077390 | 64.634 | 82 | 29 | 0 | 1 | 82 | 76 | 157 | 1.39e-31 | 108 |
| MsG0180004236.01.T03 | MTR_5g047580 | 56.790 | 81 | 35 | 0 | 1 | 81 | 76 | 156 | 4.80e-25 | 91.7 |
| MsG0180004236.01.T03 | MTR_5g047560 | 55.556 | 81 | 36 | 0 | 1 | 81 | 60 | 140 | 1.62e-24 | 89.7 |
| MsG0180004236.01.T03 | MTR_1g090697 | 46.154 | 78 | 42 | 0 | 3 | 80 | 78 | 155 | 4.43e-18 | 73.6 |
| MsG0180004236.01.T03 | MTR_1g090710 | 45.679 | 81 | 43 | 1 | 1 | 80 | 76 | 156 | 2.31e-17 | 72.0 |
| MsG0180004236.01.T03 | MTR_1g090783 | 44.304 | 79 | 44 | 0 | 2 | 80 | 76 | 154 | 2.07e-15 | 67.0 |
| MsG0180004236.01.T03 | MTR_3g065100 | 43.210 | 81 | 46 | 0 | 1 | 81 | 77 | 157 | 1.04e-12 | 61.2 |
| MsG0180004236.01.T03 | MTR_1g033370 | 43.038 | 79 | 44 | 1 | 3 | 80 | 42 | 120 | 4.02e-12 | 57.4 |
| MsG0180004236.01.T03 | MTR_1g033790 | 41.463 | 82 | 47 | 1 | 3 | 83 | 36 | 117 | 6.39e-12 | 57.0 |
| MsG0180004236.01.T03 | MTR_1g033930 | 39.024 | 82 | 49 | 1 | 3 | 83 | 36 | 117 | 9.02e-12 | 56.6 |
| MsG0180004236.01.T03 | MTR_3g466980 | 41.558 | 77 | 45 | 0 | 1 | 77 | 77 | 153 | 4.19e-11 | 56.6 |
| MsG0180004236.01.T02 | MTR_1g077320 | 95.597 | 159 | 7 | 0 | 1 | 159 | 1 | 159 | 1.48e-102 | 291 |
| MsG0180004236.01.T02 | MTR_7g011950 | 91.824 | 159 | 13 | 0 | 1 | 159 | 1 | 159 | 4.36e-98 | 280 |
| MsG0180004236.01.T02 | MTR_4g028720 | 88.679 | 159 | 18 | 0 | 1 | 159 | 1 | 159 | 7.31e-96 | 274 |
| MsG0180004236.01.T02 | MTR_1g077390 | 71.338 | 157 | 45 | 0 | 1 | 157 | 1 | 157 | 2.57e-79 | 232 |
| MsG0180004236.01.T02 | MTR_5g047580 | 69.872 | 156 | 47 | 0 | 1 | 156 | 1 | 156 | 3.02e-76 | 224 |
| MsG0180004236.01.T02 | MTR_1g077300 | 76.101 | 159 | 36 | 1 | 1 | 159 | 1 | 157 | 3.12e-76 | 224 |
| MsG0180004236.01.T02 | MTR_1g084950 | 75.000 | 156 | 39 | 0 | 1 | 156 | 1 | 156 | 4.24e-76 | 224 |
| MsG0180004236.01.T02 | MTR_1g090697 | 56.774 | 155 | 67 | 0 | 1 | 155 | 1 | 155 | 4.34e-60 | 183 |
| MsG0180004236.01.T02 | MTR_1g090710 | 55.769 | 156 | 68 | 1 | 1 | 155 | 1 | 156 | 2.48e-58 | 179 |
| MsG0180004236.01.T02 | MTR_5g047560 | 58.974 | 156 | 48 | 1 | 1 | 156 | 1 | 140 | 3.91e-58 | 178 |
| MsG0180004236.01.T02 | MTR_1g090783 | 54.902 | 153 | 69 | 0 | 3 | 155 | 2 | 154 | 4.06e-56 | 173 |
| MsG0180004236.01.T02 | MTR_3g065100 | 49.682 | 157 | 78 | 1 | 1 | 156 | 1 | 157 | 2.21e-39 | 133 |
| MsG0180004236.01.T02 | MTR_3g466980 | 45.098 | 153 | 83 | 1 | 1 | 152 | 1 | 153 | 9.02e-39 | 131 |
| MsG0180004236.01.T02 | MTR_5g075380 | 39.103 | 156 | 94 | 1 | 1 | 155 | 1 | 156 | 1.75e-37 | 126 |
| MsG0180004236.01.T02 | MTR_4g032620 | 38.065 | 155 | 94 | 2 | 1 | 153 | 1 | 155 | 8.10e-37 | 127 |
| MsG0180004236.01.T02 | MTR_4g063790 | 40.252 | 159 | 93 | 2 | 1 | 157 | 1 | 159 | 7.55e-35 | 120 |
| MsG0180004236.01.T02 | MTR_3g466830 | 42.038 | 157 | 90 | 1 | 3 | 158 | 2 | 158 | 2.21e-33 | 115 |
| MsG0180004236.01.T02 | MTR_4g031910 | 43.333 | 150 | 83 | 2 | 1 | 148 | 1 | 150 | 5.17e-32 | 114 |
| MsG0180004236.01.T02 | MTR_8g036130 | 38.994 | 159 | 93 | 2 | 1 | 155 | 1 | 159 | 1.01e-31 | 114 |
| MsG0180004236.01.T02 | MTR_3g466890 | 40.260 | 154 | 91 | 1 | 3 | 155 | 2 | 155 | 1.56e-30 | 108 |
| MsG0180004236.01.T02 | MTR_2g016210 | 41.875 | 160 | 89 | 3 | 1 | 157 | 1 | 159 | 3.03e-30 | 110 |
| MsG0180004236.01.T02 | MTR_4g032260 | 42.500 | 160 | 90 | 2 | 1 | 158 | 1 | 160 | 7.94e-30 | 107 |
| MsG0180004236.01.T02 | MTR_3g031240 | 34.969 | 163 | 96 | 3 | 1 | 157 | 1 | 159 | 6.62e-29 | 105 |
| MsG0180004236.01.T02 | MTR_4g032290 | 44.667 | 150 | 81 | 2 | 1 | 148 | 1 | 150 | 1.21e-28 | 105 |
| MsG0180004236.01.T02 | MTR_3g467080 | 35.185 | 162 | 101 | 3 | 1 | 159 | 1 | 161 | 4.51e-28 | 102 |
| MsG0180004236.01.T02 | MTR_3g031100 | 36.076 | 158 | 97 | 3 | 1 | 155 | 1 | 157 | 5.75e-28 | 104 |
| MsG0180004236.01.T02 | MTR_2g035610 | 42.373 | 118 | 67 | 1 | 1 | 117 | 1 | 118 | 2.02e-25 | 94.4 |
| MsG0180004236.01.T02 | MTR_3g465410 | 35.878 | 131 | 82 | 2 | 29 | 157 | 1 | 131 | 8.39e-22 | 85.9 |
| MsG0180004236.01.T02 | MTR_2g035580 | 32.903 | 155 | 103 | 1 | 1 | 154 | 1 | 155 | 1.06e-21 | 85.9 |
| MsG0180004236.01.T02 | MTR_3g466900 | 37.600 | 125 | 77 | 1 | 32 | 155 | 2 | 126 | 4.95e-20 | 80.9 |
| MsG0180004236.01.T02 | MTR_4g028800 | 46.575 | 73 | 37 | 1 | 1 | 73 | 1 | 71 | 7.05e-19 | 76.3 |
| MsG0180004236.01.T02 | MTR_3g466930 | 36.000 | 125 | 79 | 1 | 32 | 155 | 2 | 126 | 9.79e-19 | 77.4 |
| MsG0180004236.01.T02 | MTR_4g019670 | 35.833 | 120 | 73 | 2 | 1 | 116 | 1 | 120 | 1.72e-17 | 79.0 |
| MsG0180004236.01.T02 | MTR_7g106510 | 35.185 | 108 | 69 | 1 | 3 | 109 | 6 | 113 | 1.11e-16 | 76.3 |
| MsG0180004236.01.T02 | MTR_7g055800 | 33.333 | 117 | 51 | 2 | 1 | 117 | 1 | 90 | 2.38e-15 | 67.8 |
| MsG0180004236.01.T02 | MTR_1g033930 | 39.796 | 98 | 58 | 1 | 62 | 158 | 20 | 117 | 2.95e-14 | 65.5 |
| MsG0180004236.01.T02 | MTR_1g033370 | 42.553 | 94 | 53 | 1 | 64 | 156 | 28 | 121 | 3.09e-14 | 65.5 |
| MsG0180004236.01.T02 | MTR_1g033790 | 40.816 | 98 | 57 | 1 | 62 | 158 | 20 | 117 | 1.20e-13 | 63.9 |
| MsG0180004236.01.T02 | MTR_7g055940 | 35.238 | 105 | 41 | 3 | 1 | 105 | 1 | 78 | 6.87e-13 | 60.8 |
| MsG0180004236.01.T02 | MTR_6g005450 | 31.250 | 112 | 71 | 3 | 1 | 106 | 1 | 112 | 2.30e-12 | 63.9 |
| MsG0180004236.01.T02 | MTR_7g055790 | 34.545 | 110 | 53 | 2 | 1 | 109 | 59 | 150 | 4.11e-12 | 60.8 |
| MsG0180004236.01.T02 | MTR_3g093900 | 26.974 | 152 | 106 | 2 | 3 | 151 | 6 | 155 | 1.13e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004236.01.T01 | AT5G48670 | 38.462 | 156 | 79 | 2 | 1 | 139 | 1 | 156 | 4.38e-24 | 95.1 |
| MsG0180004236.01.T01 | AT3G05860 | 34.899 | 149 | 80 | 2 | 1 | 132 | 1 | 149 | 4.62e-20 | 82.4 |
| MsG0180004236.01.T01 | AT3G05860 | 34.899 | 149 | 80 | 2 | 1 | 132 | 1 | 149 | 1.57e-19 | 82.0 |
| MsG0180004236.01.T01 | AT3G05860 | 34.899 | 149 | 80 | 2 | 1 | 132 | 1 | 149 | 2.32e-19 | 81.6 |
| MsG0180004236.01.T01 | AT5G26630 | 33.750 | 160 | 89 | 3 | 1 | 143 | 1 | 160 | 3.16e-19 | 80.5 |
| MsG0180004236.01.T01 | AT1G65300 | 30.986 | 142 | 81 | 2 | 3 | 127 | 2 | 143 | 1.10e-16 | 74.7 |
| MsG0180004236.01.T01 | AT1G65330 | 30.612 | 147 | 85 | 2 | 3 | 132 | 2 | 148 | 3.13e-16 | 73.6 |
| MsG0180004236.01.T01 | AT5G26650 | 28.758 | 153 | 92 | 2 | 4 | 139 | 2 | 154 | 1.12e-13 | 67.0 |
| MsG0180004236.01.T01 | AT5G27810 | 39.024 | 82 | 49 | 1 | 27 | 107 | 15 | 96 | 3.40e-13 | 62.4 |
| MsG0180004236.01.T01 | AT5G27960 | 33.099 | 142 | 71 | 5 | 4 | 126 | 2 | 138 | 2.04e-12 | 63.2 |
| MsG0180004236.01.T01 | AT1G31640 | 25.806 | 155 | 97 | 3 | 3 | 140 | 2 | 155 | 1.41e-11 | 61.2 |
| MsG0180004236.01.T02 | AT5G48670 | 45.098 | 153 | 83 | 1 | 1 | 152 | 1 | 153 | 1.13e-33 | 120 |
| MsG0180004236.01.T02 | AT3G05860 | 40.940 | 149 | 87 | 1 | 1 | 148 | 1 | 149 | 2.71e-33 | 117 |
| MsG0180004236.01.T02 | AT3G05860 | 40.940 | 149 | 87 | 1 | 1 | 148 | 1 | 149 | 1.51e-32 | 116 |
| MsG0180004236.01.T02 | AT3G05860 | 40.940 | 149 | 87 | 1 | 1 | 148 | 1 | 149 | 2.71e-32 | 115 |
| MsG0180004236.01.T02 | AT5G26630 | 38.750 | 160 | 97 | 1 | 1 | 159 | 1 | 160 | 2.51e-29 | 107 |
| MsG0180004236.01.T02 | AT1G65300 | 36.620 | 142 | 89 | 1 | 3 | 143 | 2 | 143 | 4.32e-27 | 102 |
| MsG0180004236.01.T02 | AT1G65330 | 36.054 | 147 | 93 | 1 | 3 | 148 | 2 | 148 | 9.73e-27 | 102 |
| MsG0180004236.01.T02 | AT5G26650 | 34.641 | 153 | 99 | 1 | 4 | 155 | 2 | 154 | 3.01e-24 | 97.1 |
| MsG0180004236.01.T02 | AT5G27960 | 39.437 | 142 | 78 | 4 | 4 | 142 | 2 | 138 | 2.96e-23 | 93.6 |
| MsG0180004236.01.T02 | AT2G28700 | 34.868 | 152 | 92 | 2 | 1 | 148 | 1 | 149 | 7.98e-21 | 87.0 |
| MsG0180004236.01.T02 | AT1G22590 | 29.012 | 162 | 99 | 3 | 1 | 155 | 1 | 153 | 1.38e-20 | 83.2 |
| MsG0180004236.01.T02 | AT5G27810 | 42.708 | 96 | 54 | 1 | 29 | 123 | 1 | 96 | 2.63e-20 | 81.3 |
| MsG0180004236.01.T02 | AT1G31630 | 29.032 | 155 | 108 | 2 | 3 | 156 | 2 | 155 | 3.40e-20 | 85.5 |
| MsG0180004236.01.T02 | AT1G31640 | 32.000 | 150 | 100 | 2 | 3 | 151 | 2 | 150 | 8.15e-19 | 82.4 |
| MsG0180004236.01.T02 | AT5G26580 | 36.111 | 144 | 72 | 4 | 1 | 142 | 1 | 126 | 1.73e-17 | 78.2 |
| MsG0180004236.01.T02 | AT2G40210 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 1.43e-13 | 67.4 |
| MsG0180004236.01.T02 | AT5G58890 | 34.234 | 111 | 65 | 3 | 1 | 104 | 1 | 110 | 8.38e-13 | 64.7 |
| MsG0180004236.01.T02 | AT5G06500 | 29.630 | 162 | 104 | 4 | 1 | 157 | 1 | 157 | 2.64e-12 | 62.8 |
Find 23 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGAATCATTGGTGATAA+AGG | 0.252723 | 1:-75325539 | None:intergenic |
| AGCTGAACATTCGTATGATT+TGG | 0.323437 | 1:+75325845 | MsG0180004236.01.T02:CDS |
| GTTGCAAAAGGAAACTTTCC+TGG | 0.331570 | 1:-75325765 | None:intergenic |
| AGCCTTTCTTGTTGAATCAT+TGG | 0.370083 | 1:-75325548 | None:intergenic |
| CACCAATGATTCAACAAGAA+AGG | 0.413190 | 1:+75325546 | MsG0180004236.01.T02:CDS |
| AAATCTGAAGGAAGCAAAAC+AGG | 0.418438 | 1:+75325690 | MsG0180004236.01.T02:CDS |
| CTTTAGTAATCTGTTGCAAA+AGG | 0.444882 | 1:-75325777 | None:intergenic |
| TGTTTCTGATTTGAAAGACT+TGG | 0.473410 | 1:+75325903 | MsG0180004236.01.T02:CDS |
| TCAGAAACAGTCATATCATC+TGG | 0.489973 | 1:-75325889 | None:intergenic |
| TTTCACCTTCTTCCTCGTCA+TGG | 0.507566 | 1:-75325512 | None:intergenic |
| TGCAACAGATTACTAAAGCT+AGG | 0.518700 | 1:+75325782 | MsG0180004236.01.T02:CDS |
| GCAACAGATTACTAAAGCTA+GGG | 0.523992 | 1:+75325783 | MsG0180004236.01.T02:CDS |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 1:+75325610 | MsG0180004236.01.T02:CDS |
| GAAGAAAAGCATCATCAAGA+AGG | 0.545207 | 1:+75325585 | MsG0180004236.01.T02:CDS |
| GAAGGAAATTGATGACAAGA+TGG | 0.549956 | 1:+75325948 | MsG0180004236.01.T02:CDS |
| TCGGAAATTATAGCACATGC+TGG | 0.559487 | 1:-75325637 | None:intergenic |
| TGCAGCCATGACGAGGAAGA+AGG | 0.563607 | 1:+75325507 | None:intergenic |
| GAAAGGCTTCCTACAAGAAG+AGG | 0.563850 | 1:+75325563 | MsG0180004236.01.T02:CDS |
| TTCTCCTTGCAGCCATGACG+AGG | 0.566759 | 1:+75325500 | None:intergenic |
| AGAGGTGTGGCCAAATCTGA+AGG | 0.570010 | 1:+75325678 | MsG0180004236.01.T02:CDS |
| AAGCAAAACAGGTGATTGAG+AGG | 0.611042 | 1:+75325701 | MsG0180004236.01.T02:CDS |
| TTGATTCTAAAACAGAGGTG+TGG | 0.633348 | 1:+75325665 | MsG0180004236.01.T02:CDS |
| TTGGTACCTGCAAAACAACA+CGG | 0.667106 | 1:+75325864 | MsG0180004236.01.T02:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAATTTCAAAAAATATGA+AGG | + | Chr1:75325930-75325949 | MsG0180004236.01.T02:CDS | 15.0% |
| !!! | TGTTTTAGAATCAAAAGGAT+CGG | - | Chr1:75325659-75325678 | None:intergenic | 25.0% |
| CTTTAGTAATCTGTTGCAAA+AGG | - | Chr1:75325780-75325799 | None:intergenic | 30.0% | |
| ! | TGTTGAATCATTGGTGATAA+AGG | - | Chr1:75325542-75325561 | None:intergenic | 30.0% |
| ! | TGTTTCTGATTTGAAAGACT+TGG | + | Chr1:75325903-75325922 | MsG0180004236.01.T02:CDS | 30.0% |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | - | Chr1:75325664-75325683 | None:intergenic | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | + | Chr1:75325660-75325679 | MsG0180004236.01.T02:CDS | 30.0% |
| AAATCTGAAGGAAGCAAAAC+AGG | + | Chr1:75325690-75325709 | MsG0180004236.01.T02:CDS | 35.0% | |
| AGAGAACTCACTATTCTTTG+TGG | + | Chr1:75325610-75325629 | MsG0180004236.01.T02:CDS | 35.0% | |
| AGCCTTTCTTGTTGAATCAT+TGG | - | Chr1:75325551-75325570 | None:intergenic | 35.0% | |
| AGCTGAACATTCGTATGATT+TGG | + | Chr1:75325845-75325864 | MsG0180004236.01.T02:CDS | 35.0% | |
| CACCAATGATTCAACAAGAA+AGG | + | Chr1:75325546-75325565 | MsG0180004236.01.T02:CDS | 35.0% | |
| GCAACAGATTACTAAAGCTA+GGG | + | Chr1:75325783-75325802 | MsG0180004236.01.T02:CDS | 35.0% | |
| TCAGAAACAGTCATATCATC+TGG | - | Chr1:75325892-75325911 | None:intergenic | 35.0% | |
| TGAGAGAAAAAATGTGAACC+AGG | + | Chr1:75325747-75325766 | MsG0180004236.01.T02:CDS | 35.0% | |
| TGCAACAGATTACTAAAGCT+AGG | + | Chr1:75325782-75325801 | MsG0180004236.01.T02:CDS | 35.0% | |
| ! | GAAGGAAATTGATGACAAGA+TGG | + | Chr1:75325948-75325967 | MsG0180004236.01.T02:CDS | 35.0% |
| ! | TGTTTTGCTTCCTTCAGATT+TGG | - | Chr1:75325691-75325710 | None:intergenic | 35.0% |
| !! | GAAGAAAAGCATCATCAAGA+AGG | + | Chr1:75325585-75325604 | MsG0180004236.01.T02:CDS | 35.0% |
| !! | TTGATTCTAAAACAGAGGTG+TGG | + | Chr1:75325665-75325684 | MsG0180004236.01.T02:CDS | 35.0% |
| AAGCAAAACAGGTGATTGAG+AGG | + | Chr1:75325701-75325720 | MsG0180004236.01.T02:CDS | 40.0% | |
| GTTGCAAAAGGAAACTTTCC+TGG | - | Chr1:75325768-75325787 | None:intergenic | 40.0% | |
| TCGGAAATTATAGCACATGC+TGG | - | Chr1:75325640-75325659 | None:intergenic | 40.0% | |
| TTGGTACCTGCAAAACAACA+CGG | + | Chr1:75325864-75325883 | MsG0180004236.01.T02:CDS | 40.0% | |
| ! | GCTTTTCTTCCTCTTCTTGT+AGG | - | Chr1:75325575-75325594 | None:intergenic | 40.0% |
| GAAAGGCTTCCTACAAGAAG+AGG | + | Chr1:75325563-75325582 | MsG0180004236.01.T02:CDS | 45.0% | |
| ! | AGAGGTGTGGCCAAATCTGA+AGG | + | Chr1:75325678-75325697 | MsG0180004236.01.T02:CDS | 50.0% |
| !!! | CTGGCACCGTGTTGTTTTGC+AGG | - | Chr1:75325873-75325892 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 75325514 | 75325993 | 75325514 | ID=MsG0180004236.01;Name=MsG0180004236.01 |
| Chr1 | mRNA | 75325514 | 75325993 | 75325514 | ID=MsG0180004236.01.T01;Parent=MsG0180004236.01;Name=MsG0180004236.01.T01;_AED=0.09;_eAED=0.09;_QI=0|0|0|1|0|0|2|0|143 |
| Chr1 | exon | 75325514 | 75325592 | 75325514 | ID=MsG0180004236.01.T01:exon:31794;Parent=MsG0180004236.01.T01 |
| Chr1 | exon | 75325641 | 75325993 | 75325641 | ID=MsG0180004236.01.T01:exon:31795;Parent=MsG0180004236.01.T01 |
| Chr1 | CDS | 75325514 | 75325592 | 75325514 | ID=MsG0180004236.01.T01:cds;Parent=MsG0180004236.01.T01 |
| Chr1 | CDS | 75325641 | 75325993 | 75325641 | ID=MsG0180004236.01.T01:cds;Parent=MsG0180004236.01.T01 |
| Chr1 | mRNA | 75325514 | 75325993 | 75325514 | ID=MsG0180004236.01.T02;Parent=MsG0180004236.01;Name=MsG0180004236.01.T02;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|1|1|0|159 |
| Chr1 | exon | 75325514 | 75325993 | 75325514 | ID=MsG0180004236.01.T02:exon:31796;Parent=MsG0180004236.01.T02 |
| Chr1 | CDS | 75325514 | 75325993 | 75325514 | ID=MsG0180004236.01.T02:cds;Parent=MsG0180004236.01.T02 |
| Chr1 | mRNA | 75325739 | 75325993 | 75325739 | ID=MsG0180004236.01.T03;Parent=MsG0180004236.01;Name=MsG0180004236.01.T03;_AED=0.27;_eAED=0.27;_QI=0|-1|0|1|-1|0|1|0|84 |
| Chr1 | exon | 75325739 | 75325993 | 75325739 | ID=MsG0180004236.01.T03:exon:31797;Parent=MsG0180004236.01.T03 |
| Chr1 | CDS | 75325739 | 75325993 | 75325739 | ID=MsG0180004236.01.T03:cds;Parent=MsG0180004236.01.T03 |
| Gene Sequence |
| Protein sequence |
