AlfalfaGEDB | MsG0180004882.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004882.01

KEGG: K08065
GO: GO:0046982
PFAM: PF00808

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004882.01.T01	XP_013469195.1	88.66	194	13	2	1	185	1	194	3.49E-115	335
MsG0180004882.01.T02	XP_013469195.1	87.586	145	11	1	1	138	50	194	3.78E-81	247

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004882.01.T01	O82248	77.451	102	23	0	31	132	48	149	4.35E-57	179
MsG0180004882.01.T02	O82248	75.294	85	21	0	1	85	65	149	6.06E-45	147

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004882.01.T01	I3TAW4	88.660	194	13	2	1	185	1	194	1.66e-115	335
MsG0180004882.01.T02	I3TAW4	87.586	145	11	1	1	138	50	194	1.81e-81	247

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TFs/TRs:

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Gene ID	Type	Classification
MsG0180004882.01.T01	TF	NF-YB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004882.01.T01	MTR_1g088860	88.660	194	13	2	1	185	1	194	4.22e-119	335
MsG0180004882.01.T01	MTR_4g119500	78.095	105	23	0	24	128	12	116	3.08e-61	186
MsG0180004882.01.T01	MTR_0392s0020	68.992	129	37	1	24	152	8	133	1.62e-60	185
MsG0180004882.01.T01	MTR_1g083070	74.000	100	26	0	34	133	4	103	6.28e-53	165
MsG0180004882.01.T01	MTR_4g133938	64.407	118	39	1	35	152	20	134	1.29e-52	165
MsG0180004882.01.T01	MTR_2g026710	64.407	118	36	1	33	150	16	127	1.12e-51	163
MsG0180004882.01.T01	MTR_8g093920	72.277	101	28	0	33	133	39	139	1.67e-51	164
MsG0180004882.01.T01	MTR_7g061270	62.162	111	42	0	32	142	24	134	1.16e-50	161
MsG0180004882.01.T01	MTR_7g100650	62.162	111	42	0	32	142	24	134	1.51e-50	160
MsG0180004882.01.T01	MTR_3g058980	60.504	119	47	0	33	151	26	144	1.96e-50	161

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004882.01.T01	AT2G47810	77.451	102	23	0	31	132	48	149	4.43e-58	179
MsG0180004882.01.T01	AT5G47640	68.468	111	32	1	33	140	26	136	2.45e-52	166
MsG0180004882.01.T01	AT1G09030	56.429	140	57	1	32	171	1	136	2.48e-52	164
MsG0180004882.01.T01	AT4G14540	75.000	96	24	0	33	128	20	115	6.37e-52	164
MsG0180004882.01.T01	AT2G13570	73.267	101	27	0	33	133	35	135	2.57e-51	164
MsG0180004882.01.T01	AT2G38880	64.865	111	38	1	32	141	19	129	3.80e-51	161
MsG0180004882.01.T01	AT2G38880	64.865	111	38	1	32	141	19	129	4.62e-51	161
MsG0180004882.01.T01	AT2G38880	64.865	111	38	1	32	141	19	129	4.62e-51	161
MsG0180004882.01.T01	AT2G38880	64.865	111	38	1	32	141	19	129	4.62e-51	161
MsG0180004882.01.T01	AT3G53340	50.676	148	66	1	32	179	27	167	1.94e-50	160

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Find 35 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGGTATTTGTATAAGTAT+AGG	0.352771	1:-83947289	MsG0180004882.01.T01:CDS
AACAGTGACCCTCAGTCAAG+GGG	0.626356	1:-83947104	MsG0180004882.01.T01:CDS
AACATGGGTGATAACAACAA+AGG	0.619511	1:-83947635	MsG0180004882.01.T01:CDS
AAGGTATTTGTATAAGTATA+GGG	0.417767	1:-83947288	MsG0180004882.01.T01:CDS
ACATGGGTGATAACAACAAA+GGG	0.487792	1:-83947634	MsG0180004882.01.T01:CDS
AGCATCTGACAAGTGTCACA+AGG	0.642405	1:-83947393	MsG0180004882.01.T01:CDS
AGGGAATTGGATGCGGAAAG+AGG	0.523773	1:-83947269	MsG0180004882.01.T01:CDS
ATCCGACCAACATTAGCTAT+TGG	0.403010	1:+83947515	None:intergenic
ATGATGTTTGTTGGGCTTTG+GGG	0.505210	1:-83947346	MsG0180004882.01.T01:CDS
ATGGTAAGTCACAAACTCCA+AGG	0.613914	1:-83947166	MsG0180004882.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
CCTATTATTGAACATAGAAG+AGG	+	Chr1:83947605-83947624	None:intergenic	30.0%
CCTCTTCTATGTTCAATAAT+AGG	-	Chr1:83947602-83947621	MsG0180004882.01.T01:CDS	30.0%
CATGGTTTCTTTAGCATCTT+TGG	+	Chr1:83947275-83947294	None:intergenic	35.0%
GAAAGAGGGAACCAAAATAA+GGG	-	Chr1:83947475-83947494	MsG0180004882.01.T01:CDS	35.0%
TAAGTATAGGGAATTGGATG+CGG	-	Chr1:83947453-83947472	MsG0180004882.01.T01:CDS	35.0%
TTCCAATAGCTAATGTTGGT+CGG	-	Chr1:83947212-83947231	MsG0180004882.01.T01:CDS	35.0%
TTGCTTCCAATAGCTAATGT+TGG	-	Chr1:83947208-83947227	MsG0180004882.01.T01:CDS	35.0%
ATCCGACCAACATTAGCTAT+TGG	+	Chr1:83947217-83947236	None:intergenic	40.0%
ATGGTAAGTCACAAACTCCA+AGG	-	Chr1:83947563-83947582	MsG0180004882.01.T01:CDS	40.0%
GGAAAGAGGGAACCAAAATA+AGG	-	Chr1:83947474-83947493	MsG0180004882.01.T01:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	83947097	83947654	83947097	ID=MsG0180004882.01;Name=MsG0180004882.01
Chr1	mRNA	83947097	83947654	83947097	ID=MsG0180004882.01.T01;Parent=MsG0180004882.01;Name=MsG0180004882.01.T01;_AED=0.00;_eAED=0.00;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|185
Chr1	exon	83947097	83947654	83947097	ID=MsG0180004882.01.T01:exon:9089;Parent=MsG0180004882.01.T01
Chr1	CDS	83947097	83947654	83947097	ID=MsG0180004882.01.T01:cds;Parent=MsG0180004882.01.T01
Chr1	mRNA	83947097	83947513	83947097	ID=MsG0180004882.01.T02;Parent=MsG0180004882.01;Name=MsG0180004882.01.T02;_AED=0.13;_eAED=0.13;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|138
Chr1	exon	83947097	83947513	83947097	ID=MsG0180004882.01.T02:exon:9090;Parent=MsG0180004882.01.T02
Chr1	CDS	83947097	83947513	83947097	ID=MsG0180004882.01.T02:cds;Parent=MsG0180004882.01.T02

Gene Sequence

>MsG0180004882.01.T02

ATGAAACAAATCTTGCCACCAAATGCAAAAATCTCCAAAGATGCTAAAGAAACCATGCAAGAGTGTGTCTCAGAGTTTGTTAGTTTTGTAACTGGTGAAGCATCTGACAAGTGTCACAAGGAGAAGCGCAAGACAGTGAATGGTGATGATGTTTGTTGGGCTTTGGGGACACTAGGGTTTGATGATTACGCTGAGCCACTCAAAAGGTATTTGTATAAGTATAGGGAATTGGATGCGGAAAGAGGGAACCAAAATAAGGGTAACAACATCAACAACAACAGTGATGAAATTGAAAACAATATTAATGAAACATATGATTATGAAGATGGTAAGTCACAAACTCCAAGGAAAATGAATGTTGCTTCCTCTTCTATGTTCAATAATAGGAACAGTGACCCTCAGTCAAGGGGATTTTGA

>MsG0180004882.01.T01

ATGGGTGATAACAACAAAGGGAAACTAGTCTCAGATAGAGAAGTCTTTAAGTACAACACAAATCTCACATCTCATGAGCAAGATCATGAAGTAATCAAAGAGCAAGATCGTTTGCTTCCAATAGCTAATGTTGGTCGGATCATGAAACAAATCTTGCCACCAAATGCAAAAATCTCCAAAGATGCTAAAGAAACCATGCAAGAGTGTGTCTCAGAGTTTGTTAGTTTTGTAACTGGTGAAGCATCTGACAAGTGTCACAAGGAGAAGCGCAAGACAGTGAATGGTGATGATGTTTGTTGGGCTTTGGGGACACTAGGGTTTGATGATTACGCTGAGCCACTCAAAAGGTATTTGTATAAGTATAGGGAATTGGATGCGGAAAGAGGGAACCAAAATAAGGGTAACAACATCAACAACAACAGTGATGAAATTGAAAACAATATTAATGAAACATATGATTATGAAGATGGTAAGTCACAAACTCCAAGGAAAATGAATGTTGCTTCCTCTTCTATGTTCAATAATAGGAACAGTGACCCTCAGTCAAGGGGATTTTGA

Protein sequence

>MsG0180004882.01.T02

MKQILPPNAKISKDAKETMQECVSEFVSFVTGEASDKCHKEKRKTVNGDDVCWALGTLGFDDYAEPLKRYLYKYRELDAERGNQNKGNNINNNSDEIENNINETYDYEDGKSQTPRKMNVASSSMFNNRNSDPQSRGF*

>MsG0180004882.01.T01

MGDNNKGKLVSDREVFKYNTNLTSHEQDHEVIKEQDRLLPIANVGRIMKQILPPNAKISKDAKETMQECVSEFVSFVTGEASDKCHKEKRKTVNGDDVCWALGTLGFDDYAEPLKRYLYKYRELDAERGNQNKGNNINNNSDEIENNINETYDYEDGKSQTPRKMNVASSSMFNNRNSDPQSRGF*