Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004980.01.T01 | XP_013469272.1 | 83.333 | 156 | 10 | 1 | 1 | 140 | 1 | 156 | 6.17E-84 | 253 |
| MsG0180004980.01.T02 | XP_013469272.1 | 93.59 | 156 | 10 | 0 | 1 | 156 | 1 | 156 | 6.85E-101 | 296 |
| MsG0180004980.01.T03 | XP_013469272.1 | 89.552 | 67 | 7 | 0 | 1 | 67 | 90 | 156 | 5.79E-31 | 115 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004980.01.T01 | Q9FJK3 | 35.897 | 156 | 83 | 2 | 1 | 139 | 1 | 156 | 5.71E-22 | 92 |
| MsG0180004980.01.T02 | Q7XJK8 | 36.364 | 154 | 97 | 1 | 3 | 155 | 2 | 155 | 1.83E-30 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004980.01.T01 | A0A072VZ29 | 83.333 | 156 | 10 | 1 | 1 | 140 | 1 | 156 | 2.95e-84 | 253 |
| MsG0180004980.01.T02 | A0A072VZ29 | 93.590 | 156 | 10 | 0 | 1 | 156 | 1 | 156 | 3.27e-101 | 296 |
| MsG0180004980.01.T03 | A0A072VZ29 | 89.552 | 67 | 7 | 0 | 1 | 67 | 90 | 156 | 2.76e-31 | 115 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180004980.01.T02 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004980.01.T01 | MTR_1g090697 | 83.333 | 156 | 10 | 1 | 1 | 140 | 1 | 156 | 7.48e-88 | 253 |
| MsG0180004980.01.T01 | MTR_1g090710 | 64.968 | 157 | 38 | 2 | 1 | 140 | 1 | 157 | 2.26e-65 | 196 |
| MsG0180004980.01.T01 | MTR_5g047560 | 56.429 | 140 | 61 | 0 | 1 | 140 | 1 | 140 | 2.71e-51 | 160 |
| MsG0180004980.01.T01 | MTR_1g084950 | 56.129 | 155 | 52 | 1 | 1 | 139 | 1 | 155 | 8.70e-51 | 159 |
| MsG0180004980.01.T01 | MTR_1g090783 | 55.844 | 154 | 52 | 1 | 3 | 140 | 2 | 155 | 4.29e-50 | 157 |
| MsG0180004980.01.T01 | MTR_5g047580 | 52.258 | 155 | 58 | 1 | 1 | 139 | 1 | 155 | 1.44e-49 | 156 |
| MsG0180004980.01.T01 | MTR_1g077320 | 54.194 | 155 | 55 | 1 | 1 | 139 | 1 | 155 | 3.48e-49 | 155 |
| MsG0180004980.01.T01 | MTR_4g028720 | 52.258 | 155 | 58 | 1 | 1 | 139 | 1 | 155 | 5.18e-47 | 149 |
| MsG0180004980.01.T01 | MTR_7g011950 | 52.903 | 155 | 57 | 1 | 1 | 139 | 1 | 155 | 8.02e-47 | 149 |
| MsG0180004980.01.T01 | MTR_1g077390 | 48.387 | 155 | 64 | 1 | 1 | 139 | 1 | 155 | 6.36e-43 | 139 |
| MsG0180004980.01.T01 | MTR_1g077300 | 48.387 | 155 | 62 | 2 | 1 | 139 | 1 | 153 | 5.17e-38 | 127 |
| MsG0180004980.01.T01 | MTR_3g466890 | 36.774 | 155 | 81 | 2 | 3 | 140 | 2 | 156 | 7.21e-29 | 103 |
| MsG0180004980.01.T01 | MTR_3g466830 | 36.129 | 155 | 82 | 2 | 3 | 140 | 2 | 156 | 2.77e-27 | 99.8 |
| MsG0180004980.01.T01 | MTR_3g065100 | 35.256 | 156 | 84 | 2 | 1 | 139 | 1 | 156 | 5.75e-26 | 98.6 |
| MsG0180004980.01.T01 | MTR_4g031910 | 34.810 | 158 | 85 | 3 | 1 | 140 | 1 | 158 | 5.43e-24 | 92.8 |
| MsG0180004980.01.T01 | MTR_4g032620 | 31.013 | 158 | 91 | 3 | 1 | 140 | 1 | 158 | 9.23e-24 | 92.4 |
| MsG0180004980.01.T01 | MTR_3g466980 | 29.870 | 154 | 91 | 2 | 1 | 137 | 1 | 154 | 1.32e-22 | 89.4 |
| MsG0180004980.01.T01 | MTR_3g466900 | 38.261 | 115 | 70 | 1 | 27 | 140 | 13 | 127 | 5.24e-22 | 85.5 |
| MsG0180004980.01.T01 | MTR_3g466930 | 35.652 | 115 | 73 | 1 | 27 | 140 | 13 | 127 | 7.77e-20 | 79.7 |
| MsG0180004980.01.T01 | MTR_2g016210 | 35.256 | 156 | 83 | 3 | 1 | 138 | 1 | 156 | 1.06e-19 | 82.0 |
| MsG0180004980.01.T01 | MTR_4g032260 | 33.974 | 156 | 85 | 3 | 1 | 138 | 1 | 156 | 1.65e-19 | 79.7 |
| MsG0180004980.01.T01 | MTR_2g035610 | 34.711 | 121 | 62 | 2 | 1 | 104 | 1 | 121 | 1.85e-19 | 78.6 |
| MsG0180004980.01.T01 | MTR_8g036130 | 30.625 | 160 | 91 | 4 | 1 | 140 | 1 | 160 | 1.86e-19 | 81.6 |
| MsG0180004980.01.T01 | MTR_5g075380 | 31.210 | 157 | 91 | 2 | 1 | 140 | 1 | 157 | 2.38e-19 | 79.3 |
| MsG0180004980.01.T01 | MTR_3g031240 | 29.114 | 158 | 94 | 3 | 1 | 140 | 1 | 158 | 3.09e-17 | 74.3 |
| MsG0180004980.01.T01 | MTR_4g032290 | 33.544 | 158 | 87 | 3 | 1 | 140 | 1 | 158 | 3.50e-17 | 75.1 |
| MsG0180004980.01.T01 | MTR_1g033930 | 45.745 | 94 | 50 | 1 | 48 | 140 | 22 | 115 | 6.14e-17 | 72.0 |
| MsG0180004980.01.T01 | MTR_4g063790 | 29.487 | 156 | 88 | 4 | 1 | 136 | 1 | 154 | 1.62e-16 | 72.0 |
| MsG0180004980.01.T01 | MTR_1g033370 | 37.755 | 98 | 60 | 1 | 44 | 140 | 24 | 121 | 3.57e-15 | 67.4 |
| MsG0180004980.01.T01 | MTR_1g033790 | 42.553 | 94 | 53 | 1 | 48 | 140 | 22 | 115 | 1.18e-13 | 63.5 |
| MsG0180004980.01.T01 | MTR_3g031100 | 29.936 | 157 | 92 | 4 | 1 | 139 | 1 | 157 | 1.34e-13 | 65.9 |
| MsG0180004980.01.T02 | MTR_1g090697 | 93.590 | 156 | 10 | 0 | 1 | 156 | 1 | 156 | 8.29e-105 | 296 |
| MsG0180004980.01.T02 | MTR_1g090710 | 72.611 | 157 | 42 | 1 | 1 | 156 | 1 | 157 | 2.78e-80 | 234 |
| MsG0180004980.01.T02 | MTR_5g047580 | 60.000 | 155 | 62 | 0 | 1 | 155 | 1 | 155 | 2.04e-63 | 192 |
| MsG0180004980.01.T02 | MTR_1g090783 | 62.338 | 154 | 58 | 0 | 3 | 156 | 2 | 155 | 2.83e-63 | 191 |
| MsG0180004980.01.T02 | MTR_1g084950 | 63.871 | 155 | 56 | 0 | 1 | 155 | 1 | 155 | 5.90e-61 | 185 |
| MsG0180004980.01.T02 | MTR_1g077320 | 61.290 | 155 | 60 | 0 | 1 | 155 | 1 | 155 | 4.83e-59 | 181 |
| MsG0180004980.01.T02 | MTR_1g077390 | 57.419 | 155 | 66 | 0 | 1 | 155 | 1 | 155 | 1.05e-58 | 180 |
| MsG0180004980.01.T02 | MTR_4g028720 | 60.000 | 155 | 62 | 0 | 1 | 155 | 1 | 155 | 1.10e-56 | 175 |
| MsG0180004980.01.T02 | MTR_7g011950 | 60.000 | 155 | 62 | 0 | 1 | 155 | 1 | 155 | 1.29e-56 | 174 |
| MsG0180004980.01.T02 | MTR_1g077300 | 56.129 | 155 | 66 | 1 | 1 | 155 | 1 | 153 | 1.51e-47 | 151 |
| MsG0180004980.01.T02 | MTR_5g047560 | 50.968 | 155 | 60 | 1 | 1 | 155 | 1 | 139 | 1.09e-46 | 149 |
| MsG0180004980.01.T02 | MTR_3g466830 | 43.871 | 155 | 86 | 1 | 3 | 156 | 2 | 156 | 7.32e-40 | 132 |
| MsG0180004980.01.T02 | MTR_3g466890 | 42.581 | 155 | 88 | 1 | 3 | 156 | 2 | 156 | 9.69e-38 | 127 |
| MsG0180004980.01.T02 | MTR_4g032620 | 37.342 | 158 | 97 | 2 | 1 | 156 | 1 | 158 | 4.18e-36 | 124 |
| MsG0180004980.01.T02 | MTR_3g466980 | 36.364 | 154 | 97 | 1 | 1 | 153 | 1 | 154 | 1.19e-34 | 120 |
| MsG0180004980.01.T02 | MTR_3g065100 | 42.308 | 156 | 89 | 1 | 1 | 155 | 1 | 156 | 1.31e-34 | 121 |
| MsG0180004980.01.T02 | MTR_4g031910 | 41.772 | 158 | 90 | 2 | 1 | 156 | 1 | 158 | 2.85e-32 | 115 |
| MsG0180004980.01.T02 | MTR_2g016210 | 41.667 | 156 | 89 | 2 | 1 | 154 | 1 | 156 | 9.37e-32 | 114 |
| MsG0180004980.01.T02 | MTR_8g036130 | 36.875 | 160 | 97 | 3 | 1 | 156 | 1 | 160 | 3.03e-31 | 112 |
| MsG0180004980.01.T02 | MTR_5g075380 | 35.669 | 157 | 100 | 1 | 1 | 156 | 1 | 157 | 1.18e-29 | 106 |
| MsG0180004980.01.T02 | MTR_4g063790 | 33.974 | 156 | 97 | 3 | 1 | 152 | 1 | 154 | 8.27e-28 | 102 |
| MsG0180004980.01.T02 | MTR_4g032260 | 41.026 | 156 | 90 | 2 | 1 | 154 | 1 | 156 | 1.38e-27 | 100 |
| MsG0180004980.01.T02 | MTR_3g466900 | 41.270 | 126 | 73 | 1 | 32 | 156 | 2 | 127 | 1.49e-27 | 100 |
| MsG0180004980.01.T02 | MTR_2g035610 | 42.975 | 121 | 68 | 1 | 1 | 120 | 1 | 121 | 1.95e-27 | 99.4 |
| MsG0180004980.01.T02 | MTR_3g031240 | 33.544 | 158 | 103 | 2 | 1 | 156 | 1 | 158 | 2.15e-27 | 101 |
| MsG0180004980.01.T02 | MTR_3g466930 | 38.889 | 126 | 76 | 1 | 32 | 156 | 2 | 127 | 2.03e-25 | 94.7 |
| MsG0180004980.01.T02 | MTR_4g032290 | 40.506 | 158 | 92 | 2 | 1 | 156 | 1 | 158 | 2.96e-25 | 97.1 |
| MsG0180004980.01.T02 | MTR_3g031100 | 33.121 | 157 | 103 | 2 | 1 | 155 | 1 | 157 | 9.92e-25 | 95.9 |
| MsG0180004980.01.T02 | MTR_4g028800 | 51.389 | 72 | 33 | 1 | 1 | 72 | 1 | 70 | 6.84e-23 | 86.3 |
| MsG0180004980.01.T02 | MTR_3g467080 | 29.299 | 157 | 109 | 2 | 1 | 155 | 1 | 157 | 1.47e-20 | 82.8 |
| MsG0180004980.01.T02 | MTR_2g035580 | 29.032 | 155 | 109 | 1 | 1 | 154 | 1 | 155 | 7.59e-18 | 75.9 |
| MsG0180004980.01.T02 | MTR_4g019670 | 28.834 | 163 | 98 | 4 | 1 | 145 | 1 | 163 | 3.94e-17 | 77.8 |
| MsG0180004980.01.T02 | MTR_7g106510 | 29.878 | 164 | 95 | 6 | 1 | 145 | 1 | 163 | 9.58e-17 | 76.6 |
| MsG0180004980.01.T02 | MTR_1g033930 | 45.745 | 94 | 50 | 1 | 64 | 156 | 22 | 115 | 1.21e-16 | 71.6 |
| MsG0180004980.01.T02 | MTR_3g093900 | 29.677 | 155 | 108 | 1 | 3 | 156 | 6 | 160 | 1.67e-16 | 75.9 |
| MsG0180004980.01.T02 | MTR_1g033370 | 37.755 | 98 | 60 | 1 | 60 | 156 | 24 | 121 | 7.06e-15 | 67.0 |
| MsG0180004980.01.T02 | MTR_3g465410 | 28.906 | 128 | 85 | 3 | 29 | 152 | 1 | 126 | 4.39e-14 | 65.9 |
| MsG0180004980.01.T02 | MTR_7g055800 | 29.661 | 118 | 56 | 2 | 1 | 118 | 1 | 91 | 6.85e-14 | 63.9 |
| MsG0180004980.01.T02 | MTR_1g033790 | 42.553 | 94 | 53 | 1 | 64 | 156 | 22 | 115 | 2.25e-13 | 63.2 |
| MsG0180004980.01.T02 | MTR_6g005450 | 30.469 | 128 | 76 | 4 | 1 | 115 | 1 | 128 | 9.63e-12 | 62.0 |
| MsG0180004980.01.T02 | MTR_6g018920 | 24.845 | 161 | 108 | 6 | 1 | 155 | 1 | 154 | 2.15e-11 | 60.8 |
| MsG0180004980.01.T02 | MTR_7g075850 | 31.373 | 153 | 86 | 4 | 1 | 148 | 1 | 139 | 2.66e-11 | 60.1 |
| MsG0180004980.01.T03 | MTR_1g090697 | 89.552 | 67 | 7 | 0 | 1 | 67 | 90 | 156 | 7.01e-35 | 115 |
| MsG0180004980.01.T03 | MTR_1g090710 | 69.118 | 68 | 20 | 1 | 1 | 67 | 90 | 157 | 1.13e-23 | 87.4 |
| MsG0180004980.01.T03 | MTR_1g077320 | 50.000 | 66 | 33 | 0 | 1 | 66 | 90 | 155 | 6.20e-16 | 67.4 |
| MsG0180004980.01.T03 | MTR_1g084950 | 51.515 | 66 | 32 | 0 | 1 | 66 | 90 | 155 | 1.50e-15 | 66.6 |
| MsG0180004980.01.T03 | MTR_4g028720 | 51.515 | 66 | 32 | 0 | 1 | 66 | 90 | 155 | 1.60e-15 | 66.6 |
| MsG0180004980.01.T03 | MTR_1g090783 | 53.731 | 67 | 31 | 0 | 1 | 67 | 89 | 155 | 2.31e-15 | 65.9 |
| MsG0180004980.01.T03 | MTR_7g011950 | 48.485 | 66 | 34 | 0 | 1 | 66 | 90 | 155 | 1.91e-14 | 63.5 |
| MsG0180004980.01.T03 | MTR_5g047580 | 48.485 | 66 | 34 | 0 | 1 | 66 | 90 | 155 | 3.55e-13 | 60.5 |
| MsG0180004980.01.T03 | MTR_1g077390 | 45.455 | 66 | 36 | 0 | 1 | 66 | 90 | 155 | 1.00e-11 | 56.6 |
| MsG0180004980.01.T03 | MTR_5g047560 | 45.455 | 66 | 36 | 0 | 1 | 66 | 74 | 139 | 2.65e-11 | 55.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004980.01.T01 | AT5G48670 | 35.897 | 156 | 83 | 2 | 1 | 139 | 1 | 156 | 5.82e-23 | 92.0 |
| MsG0180004980.01.T01 | AT3G05860 | 36.242 | 149 | 78 | 2 | 1 | 132 | 1 | 149 | 2.79e-21 | 85.5 |
| MsG0180004980.01.T01 | AT5G26630 | 32.692 | 156 | 88 | 3 | 1 | 139 | 1 | 156 | 3.86e-21 | 85.5 |
| MsG0180004980.01.T01 | AT3G05860 | 36.242 | 149 | 78 | 2 | 1 | 132 | 1 | 149 | 9.61e-21 | 85.1 |
| MsG0180004980.01.T01 | AT3G05860 | 36.242 | 149 | 78 | 2 | 1 | 132 | 1 | 149 | 1.21e-20 | 84.7 |
| MsG0180004980.01.T01 | AT1G65300 | 30.519 | 154 | 90 | 2 | 3 | 139 | 2 | 155 | 4.14e-20 | 84.0 |
| MsG0180004980.01.T01 | AT1G65330 | 31.169 | 154 | 89 | 2 | 3 | 139 | 2 | 155 | 3.13e-18 | 79.0 |
| MsG0180004980.01.T01 | AT1G31640 | 29.333 | 150 | 88 | 3 | 3 | 135 | 2 | 150 | 1.07e-15 | 72.8 |
| MsG0180004980.01.T01 | AT5G26650 | 30.065 | 153 | 90 | 2 | 4 | 139 | 2 | 154 | 8.58e-15 | 70.1 |
| MsG0180004980.01.T01 | AT1G22590 | 32.278 | 158 | 85 | 4 | 1 | 140 | 1 | 154 | 1.00e-14 | 67.4 |
| MsG0180004980.01.T01 | AT1G31630 | 26.490 | 151 | 93 | 3 | 3 | 136 | 2 | 151 | 1.55e-14 | 69.3 |
| MsG0180004980.01.T01 | AT5G27810 | 36.585 | 82 | 51 | 1 | 27 | 107 | 15 | 96 | 1.98e-13 | 62.8 |
| MsG0180004980.01.T01 | AT5G27960 | 28.758 | 153 | 92 | 2 | 4 | 139 | 2 | 154 | 5.06e-13 | 65.1 |
| MsG0180004980.01.T02 | AT3G05860 | 42.282 | 149 | 85 | 1 | 1 | 148 | 1 | 149 | 4.15e-33 | 116 |
| MsG0180004980.01.T02 | AT3G05860 | 42.282 | 149 | 85 | 1 | 1 | 148 | 1 | 149 | 2.31e-32 | 115 |
| MsG0180004980.01.T02 | AT3G05860 | 42.282 | 149 | 85 | 1 | 1 | 148 | 1 | 149 | 3.23e-32 | 115 |
| MsG0180004980.01.T02 | AT1G65300 | 36.364 | 154 | 97 | 1 | 3 | 155 | 2 | 155 | 1.87e-31 | 114 |
| MsG0180004980.01.T02 | AT5G48670 | 43.590 | 156 | 87 | 1 | 1 | 155 | 1 | 156 | 7.34e-31 | 113 |
| MsG0180004980.01.T02 | AT1G65330 | 36.364 | 154 | 97 | 1 | 3 | 155 | 2 | 155 | 5.95e-29 | 107 |
| MsG0180004980.01.T02 | AT5G26630 | 37.821 | 156 | 96 | 1 | 1 | 155 | 1 | 156 | 1.39e-28 | 105 |
| MsG0180004980.01.T02 | AT1G22590 | 38.608 | 158 | 91 | 3 | 1 | 156 | 1 | 154 | 1.29e-27 | 101 |
| MsG0180004980.01.T02 | AT5G26650 | 35.294 | 153 | 98 | 1 | 4 | 155 | 2 | 154 | 7.57e-25 | 98.2 |
| MsG0180004980.01.T02 | AT1G31640 | 36.000 | 150 | 94 | 2 | 3 | 151 | 2 | 150 | 1.41e-24 | 98.6 |
| MsG0180004980.01.T02 | AT1G31630 | 31.126 | 151 | 102 | 2 | 3 | 152 | 2 | 151 | 4.72e-24 | 95.9 |
| MsG0180004980.01.T02 | AT5G27960 | 33.987 | 153 | 100 | 1 | 4 | 155 | 2 | 154 | 3.18e-23 | 93.2 |
| MsG0180004980.01.T02 | AT2G28700 | 33.071 | 127 | 78 | 2 | 1 | 123 | 1 | 124 | 3.77e-20 | 85.1 |
| MsG0180004980.01.T02 | AT5G27810 | 40.625 | 96 | 56 | 1 | 29 | 123 | 1 | 96 | 6.27e-20 | 80.1 |
| MsG0180004980.01.T02 | AT5G06500 | 35.526 | 152 | 84 | 5 | 1 | 145 | 1 | 145 | 3.39e-19 | 81.3 |
| MsG0180004980.01.T02 | AT5G26580 | 29.677 | 155 | 96 | 1 | 1 | 155 | 1 | 142 | 2.55e-16 | 74.7 |
| MsG0180004980.01.T02 | AT2G40210 | 32.857 | 140 | 88 | 3 | 1 | 137 | 1 | 137 | 1.57e-12 | 64.3 |
Find 24 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTACCTTCTTGGCTTCTTC+TGG | 0.189716 | 1:+85144157 | None:intergenic |
| ATACCTCTCAATTACCTTCT+TGG | 0.215921 | 1:+85144147 | None:intergenic |
| TGCCTTCCTTGCCGCGTTAT+CGG | 0.274204 | 1:+85144303 | None:intergenic |
| GACAACACTTTGTGATATTC+CGG | 0.300516 | 1:-85144233 | MsG0180004980.01.T02:CDS |
| TTCTTGGCTTCTTCTGGATT+CGG | 0.341741 | 1:+85144163 | None:intergenic |
| TCTGCATAATGTATCTTTCA+TGG | 0.373491 | 1:+85144086 | None:intergenic |
| TTGATTCTCAGACTGAGGTT+TGG | 0.414602 | 1:-85144186 | MsG0180004980.01.T02:CDS |
| TGCCGCGTTATCGGAGATAA+AGG | 0.416769 | 1:+85144312 | None:intergenic |
| CTCCGATAACGCGGCAAGGA+AGG | 0.449540 | 1:-85144305 | MsG0180004980.01.T02:CDS |
| AAGCTACAATAAAAGAAAGA+AGG | 0.492405 | 1:-85144281 | MsG0180004980.01.T02:CDS |
| TTTCACCTTCCTTCTCGTCA+TGG | 0.513068 | 1:+85144339 | None:intergenic |
| ACCTCAGTCTGAGAATCAAA+AGG | 0.514840 | 1:+85144190 | None:intergenic |
| GCATGTTGAGAAGAAGTTGA+AGG | 0.520532 | 1:-85143921 | MsG0180004980.01.T02:CDS |
| AGTCTGAGAATCAAAAGGAC+TGG | 0.521393 | 1:+85144195 | None:intergenic |
| TGCAGATGATTGCTAAAGCA+AGG | 0.534319 | 1:-85144069 | MsG0180004980.01.T02:CDS |
| AGCTACAATAAAAGAAAGAA+GGG | 0.567899 | 1:-85144280 | MsG0180004980.01.T02:CDS |
| TGTTGAGAAGAAGTTGAAGG+AGG | 0.578028 | 1:-85143918 | MsG0180004980.01.T02:CDS |
| TGCAACCATGACGAGAAGGA+AGG | 0.595441 | 1:-85144344 | None:intergenic |
| GAATCCAGAAGAAGCCAAGA+AGG | 0.598410 | 1:-85144161 | MsG0180004980.01.T02:CDS |
| TGCCTTTATCTCCGATAACG+CGG | 0.615017 | 1:-85144314 | MsG0180004980.01.T02:CDS |
| TTATCTCCGATAACGCGGCA+AGG | 0.616944 | 1:-85144309 | MsG0180004980.01.T02:CDS |
| CTGGAAATTATAGCACAAGC+CGG | 0.619015 | 1:+85144214 | None:intergenic |
| AAGCCAAGAAGGTAATTGAG+AGG | 0.625836 | 1:-85144150 | MsG0180004980.01.T02:CDS |
| GCAGATGATTGCTAAAGCAA+GGG | 0.627505 | 1:-85144068 | MsG0180004980.01.T02:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGCTACAATAAAAGAAAGA+AGG | - | Chr1:85143945-85143964 | MsG0180004980.01.T02:CDS | 25.0% |
| ! | AGCTACAATAAAAGAAAGAA+GGG | - | Chr1:85143946-85143965 | MsG0180004980.01.T02:CDS | 25.0% |
| AAAGAAGGGTCTCATAAAAA+AGG | - | Chr1:85143960-85143979 | MsG0180004980.01.T02:CDS | 30.0% | |
| TCTGCATAATGTATCTTTCA+TGG | + | Chr1:85144143-85144162 | None:intergenic | 30.0% | |
| ! | AGCTGAATCTTTTTATGCTA+CGG | - | Chr1:85144220-85144239 | MsG0180004980.01.T02:CDS | 30.0% |
| ATACCTCTCAATTACCTTCT+TGG | + | Chr1:85144082-85144101 | None:intergenic | 35.0% | |
| GACAACACTTTGTGATATTC+CGG | - | Chr1:85143993-85144012 | MsG0180004980.01.T02:CDS | 35.0% | |
| ! | TTTTCAGTTCTTCAGCAGTT+AGG | + | Chr1:85144275-85144294 | None:intergenic | 35.0% |
| AAGCCAAGAAGGTAATTGAG+AGG | - | Chr1:85144076-85144095 | MsG0180004980.01.T02:CDS | 40.0% | |
| ACCTCAGTCTGAGAATCAAA+AGG | + | Chr1:85144039-85144058 | None:intergenic | 40.0% | |
| AGTCTGAGAATCAAAAGGAC+TGG | + | Chr1:85144034-85144053 | None:intergenic | 40.0% | |
| ATTACCTTCTTGGCTTCTTC+TGG | + | Chr1:85144072-85144091 | None:intergenic | 40.0% | |
| CTGGAAATTATAGCACAAGC+CGG | + | Chr1:85144015-85144034 | None:intergenic | 40.0% | |
| GCAGATGATTGCTAAAGCAA+GGG | - | Chr1:85144158-85144177 | MsG0180004980.01.T02:CDS | 40.0% | |
| GCATGTTGAGAAGAAGTTGA+AGG | - | Chr1:85144305-85144324 | MsG0180004980.01.T02:CDS | 40.0% | |
| TGCAGATGATTGCTAAAGCA+AGG | - | Chr1:85144157-85144176 | MsG0180004980.01.T02:CDS | 40.0% | |
| TGTTGAGAAGAAGTTGAAGG+AGG | - | Chr1:85144308-85144327 | MsG0180004980.01.T02:CDS | 40.0% | |
| TTCTTGGCTTCTTCTGGATT+CGG | + | Chr1:85144066-85144085 | None:intergenic | 40.0% | |
| TTGATTCTCAGACTGAGGTT+TGG | - | Chr1:85144040-85144059 | MsG0180004980.01.T02:CDS | 40.0% | |
| !! | TCCTTTTGATTCTCAGACTG+AGG | - | Chr1:85144035-85144054 | MsG0180004980.01.T02:CDS | 40.0% |
| GAATCCAGAAGAAGCCAAGA+AGG | - | Chr1:85144065-85144084 | MsG0180004980.01.T02:CDS | 45.0% | |
| TGCCTTTATCTCCGATAACG+CGG | - | Chr1:85143912-85143931 | MsG0180004980.01.T02:CDS | 45.0% | |
| TGCCGCGTTATCGGAGATAA+AGG | + | Chr1:85143917-85143936 | None:intergenic | 50.0% | |
| TTATCTCCGATAACGCGGCA+AGG | - | Chr1:85143917-85143936 | MsG0180004980.01.T02:CDS | 50.0% | |
| TGCCTTCCTTGCCGCGTTAT+CGG | + | Chr1:85143926-85143945 | None:intergenic | 55.0% | |
| CTCCGATAACGCGGCAAGGA+AGG | - | Chr1:85143921-85143940 | MsG0180004980.01.T02:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 85143889 | 85144359 | 85143889 | ID=MsG0180004980.01;Name=MsG0180004980.01 |
| Chr1 | mRNA | 85143889 | 85144359 | 85143889 | ID=MsG0180004980.01.T01;Parent=MsG0180004980.01;Name=MsG0180004980.01.T01;_AED=0.10;_eAED=0.10;_QI=0|0|0|1|0|0|2|0|140 |
| Chr1 | exon | 85143889 | 85144232 | 85143889 | ID=MsG0180004980.01.T01:exon:9559;Parent=MsG0180004980.01.T01 |
| Chr1 | exon | 85144281 | 85144359 | 85144281 | ID=MsG0180004980.01.T01:exon:9558;Parent=MsG0180004980.01.T01 |
| Chr1 | CDS | 85144281 | 85144359 | 85144281 | ID=MsG0180004980.01.T01:cds;Parent=MsG0180004980.01.T01 |
| Chr1 | CDS | 85143889 | 85144232 | 85143889 | ID=MsG0180004980.01.T01:cds;Parent=MsG0180004980.01.T01 |
| Chr1 | mRNA | 85143889 | 85144359 | 85143889 | ID=MsG0180004980.01.T02;Parent=MsG0180004980.01;Name=MsG0180004980.01.T02;_AED=0.06;_eAED=0.06;_QI=0|-1|0|1|-1|1|1|0|156 |
| Chr1 | exon | 85143889 | 85144359 | 85143889 | ID=MsG0180004980.01.T02:exon:9560;Parent=MsG0180004980.01.T02 |
| Chr1 | CDS | 85143889 | 85144359 | 85143889 | ID=MsG0180004980.01.T02:cds;Parent=MsG0180004980.01.T02 |
| Chr1 | mRNA | 85143889 | 85144092 | 85143889 | ID=MsG0180004980.01.T03;Parent=MsG0180004980.01;Name=MsG0180004980.01.T03;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|0|1|0|67 |
| Chr1 | exon | 85143889 | 85144092 | 85143889 | ID=MsG0180004980.01.T03:exon:9561;Parent=MsG0180004980.01.T03 |
| Chr1 | CDS | 85143889 | 85144092 | 85143889 | ID=MsG0180004980.01.T03:cds;Parent=MsG0180004980.01.T03 |
| Gene Sequence |
| Protein sequence |
