Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004983.01.T01 | XP_013469276.1 | 88.535 | 157 | 17 | 1 | 1 | 156 | 1 | 157 | 3.03E-95 | 282 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004983.01.T01 | Q9FJK3 | 42.308 | 156 | 89 | 1 | 1 | 155 | 1 | 156 | 3.31E-30 | 114 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004983.01.T01 | A0A072VMS9 | 88.535 | 157 | 17 | 1 | 1 | 156 | 1 | 157 | 1.45e-95 | 282 |
| Gene ID | Type | Classification |
|---|---|---|
| MsG0180004983.01.T01 | TF | MADS-M-type |
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004983.01.T01 | MTR_1g090710 | 88.535 | 157 | 17 | 1 | 1 | 156 | 1 | 157 | 3.67e-99 | 282 |
| MsG0180004983.01.T01 | MTR_1g090697 | 71.154 | 156 | 45 | 0 | 1 | 156 | 1 | 156 | 2.27e-80 | 234 |
| MsG0180004983.01.T01 | MTR_5g047580 | 55.484 | 155 | 69 | 0 | 1 | 155 | 1 | 155 | 1.52e-58 | 179 |
| MsG0180004983.01.T01 | MTR_1g090783 | 57.792 | 154 | 65 | 0 | 3 | 156 | 2 | 155 | 4.59e-58 | 178 |
| MsG0180004983.01.T01 | MTR_1g077390 | 52.903 | 155 | 73 | 0 | 1 | 155 | 1 | 155 | 3.03e-55 | 171 |
| MsG0180004983.01.T01 | MTR_1g084950 | 56.129 | 155 | 68 | 0 | 1 | 155 | 1 | 155 | 6.31e-52 | 162 |
| MsG0180004983.01.T01 | MTR_1g077320 | 52.903 | 155 | 73 | 0 | 1 | 155 | 1 | 155 | 1.70e-49 | 156 |
| MsG0180004983.01.T01 | MTR_7g011950 | 50.968 | 155 | 76 | 0 | 1 | 155 | 1 | 155 | 5.61e-47 | 150 |
| MsG0180004983.01.T01 | MTR_4g028720 | 50.968 | 155 | 76 | 0 | 1 | 155 | 1 | 155 | 1.10e-46 | 149 |
| MsG0180004983.01.T01 | MTR_1g077300 | 50.323 | 155 | 75 | 1 | 1 | 155 | 1 | 153 | 6.03e-42 | 137 |
| MsG0180004983.01.T01 | MTR_5g047560 | 45.806 | 155 | 68 | 1 | 1 | 155 | 1 | 139 | 1.92e-40 | 133 |
| MsG0180004983.01.T01 | MTR_3g466890 | 42.581 | 155 | 88 | 1 | 3 | 156 | 2 | 156 | 1.46e-36 | 124 |
| MsG0180004983.01.T01 | MTR_3g466830 | 40.645 | 155 | 91 | 1 | 3 | 156 | 2 | 156 | 8.66e-36 | 122 |
| MsG0180004983.01.T01 | MTR_3g065100 | 41.667 | 156 | 90 | 1 | 1 | 155 | 1 | 156 | 6.44e-35 | 122 |
| MsG0180004983.01.T01 | MTR_4g032620 | 35.443 | 158 | 100 | 2 | 1 | 156 | 1 | 158 | 1.79e-34 | 120 |
| MsG0180004983.01.T01 | MTR_3g466980 | 36.364 | 154 | 97 | 1 | 1 | 153 | 1 | 154 | 7.00e-34 | 119 |
| MsG0180004983.01.T01 | MTR_4g031910 | 42.405 | 158 | 89 | 2 | 1 | 156 | 1 | 158 | 1.02e-33 | 118 |
| MsG0180004983.01.T01 | MTR_5g075380 | 38.854 | 157 | 95 | 1 | 1 | 156 | 1 | 157 | 2.57e-32 | 113 |
| MsG0180004983.01.T01 | MTR_8g036130 | 34.375 | 160 | 101 | 2 | 1 | 156 | 1 | 160 | 1.18e-30 | 111 |
| MsG0180004983.01.T01 | MTR_2g016210 | 35.256 | 156 | 99 | 2 | 1 | 154 | 1 | 156 | 1.45e-27 | 103 |
| MsG0180004983.01.T01 | MTR_4g032260 | 40.385 | 156 | 91 | 2 | 1 | 154 | 1 | 156 | 2.76e-27 | 100 |
| MsG0180004983.01.T01 | MTR_4g063790 | 36.306 | 157 | 92 | 3 | 1 | 152 | 1 | 154 | 6.03e-27 | 99.8 |
| MsG0180004983.01.T01 | MTR_3g031240 | 35.220 | 159 | 99 | 3 | 1 | 156 | 1 | 158 | 2.34e-26 | 98.6 |
| MsG0180004983.01.T01 | MTR_3g031100 | 34.177 | 158 | 100 | 3 | 1 | 155 | 1 | 157 | 1.52e-25 | 98.2 |
| MsG0180004983.01.T01 | MTR_2g035610 | 40.496 | 121 | 71 | 1 | 1 | 120 | 1 | 121 | 1.84e-25 | 94.4 |
| MsG0180004983.01.T01 | MTR_4g032290 | 40.506 | 158 | 92 | 2 | 1 | 156 | 1 | 158 | 3.22e-25 | 97.1 |
| MsG0180004983.01.T01 | MTR_3g466930 | 38.095 | 126 | 77 | 1 | 32 | 156 | 2 | 127 | 5.32e-24 | 90.9 |
| MsG0180004983.01.T01 | MTR_3g467080 | 31.447 | 159 | 103 | 3 | 1 | 155 | 1 | 157 | 2.13e-23 | 90.5 |
| MsG0180004983.01.T01 | MTR_3g466900 | 38.095 | 126 | 77 | 1 | 32 | 156 | 2 | 127 | 2.31e-23 | 89.4 |
| MsG0180004983.01.T01 | MTR_4g028800 | 50.685 | 73 | 34 | 1 | 1 | 73 | 1 | 71 | 3.68e-23 | 87.0 |
| MsG0180004983.01.T01 | MTR_2g035580 | 34.737 | 95 | 61 | 1 | 1 | 94 | 1 | 95 | 5.03e-19 | 79.0 |
| MsG0180004983.01.T01 | MTR_4g019670 | 34.259 | 108 | 70 | 1 | 3 | 109 | 6 | 113 | 6.23e-18 | 80.1 |
| MsG0180004983.01.T01 | MTR_7g106510 | 32.407 | 108 | 72 | 1 | 3 | 109 | 6 | 113 | 1.14e-16 | 76.3 |
| MsG0180004983.01.T01 | MTR_7g055800 | 28.814 | 118 | 57 | 2 | 1 | 118 | 1 | 91 | 5.55e-15 | 66.6 |
| MsG0180004983.01.T01 | MTR_3g093900 | 26.452 | 155 | 113 | 1 | 3 | 156 | 6 | 160 | 2.15e-14 | 69.7 |
| MsG0180004983.01.T01 | MTR_6g005450 | 30.120 | 166 | 97 | 6 | 1 | 157 | 1 | 156 | 3.23e-14 | 68.9 |
| MsG0180004983.01.T01 | MTR_3g465410 | 30.469 | 128 | 83 | 3 | 29 | 152 | 1 | 126 | 4.60e-14 | 65.5 |
| MsG0180004983.01.T01 | MTR_6g018920 | 26.506 | 166 | 103 | 5 | 1 | 157 | 1 | 156 | 9.21e-14 | 67.8 |
| MsG0180004983.01.T01 | MTR_1g033930 | 39.362 | 94 | 56 | 1 | 64 | 156 | 22 | 115 | 1.75e-13 | 63.5 |
| MsG0180004983.01.T01 | MTR_7g075850 | 29.605 | 152 | 96 | 4 | 1 | 151 | 1 | 142 | 7.32e-12 | 61.6 |
| MsG0180004983.01.T01 | MTR_1g114730 | 27.329 | 161 | 104 | 4 | 1 | 155 | 1 | 154 | 1.47e-11 | 61.6 |
| MsG0180004983.01.T01 | MTR_7g055790 | 30.631 | 111 | 58 | 2 | 1 | 110 | 59 | 151 | 7.33e-11 | 57.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004983.01.T01 | AT5G48670 | 42.308 | 156 | 89 | 1 | 1 | 155 | 1 | 156 | 3.37e-31 | 114 |
| MsG0180004983.01.T01 | AT5G26630 | 40.385 | 156 | 92 | 1 | 1 | 155 | 1 | 156 | 1.33e-30 | 110 |
| MsG0180004983.01.T01 | AT1G65300 | 37.013 | 154 | 96 | 1 | 3 | 155 | 2 | 155 | 1.97e-30 | 111 |
| MsG0180004983.01.T01 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 6.54e-30 | 108 |
| MsG0180004983.01.T01 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 3.16e-29 | 107 |
| MsG0180004983.01.T01 | AT3G05860 | 38.926 | 149 | 90 | 1 | 1 | 148 | 1 | 149 | 3.88e-29 | 107 |
| MsG0180004983.01.T01 | AT1G65330 | 37.662 | 154 | 95 | 1 | 3 | 155 | 2 | 155 | 7.12e-28 | 104 |
| MsG0180004983.01.T01 | AT1G31640 | 35.333 | 150 | 95 | 2 | 3 | 151 | 2 | 150 | 1.00e-25 | 101 |
| MsG0180004983.01.T01 | AT1G22590 | 34.177 | 158 | 100 | 2 | 1 | 157 | 1 | 155 | 4.14e-25 | 94.7 |
| MsG0180004983.01.T01 | AT5G26650 | 34.641 | 153 | 99 | 1 | 4 | 155 | 2 | 154 | 7.43e-25 | 98.6 |
| MsG0180004983.01.T01 | AT5G27960 | 34.641 | 153 | 99 | 1 | 4 | 155 | 2 | 154 | 2.87e-24 | 96.3 |
| MsG0180004983.01.T01 | AT2G28700 | 40.000 | 110 | 65 | 1 | 1 | 109 | 1 | 110 | 2.18e-23 | 94.0 |
| MsG0180004983.01.T01 | AT5G06500 | 35.443 | 158 | 94 | 2 | 1 | 154 | 1 | 154 | 7.67e-22 | 88.2 |
| MsG0180004983.01.T01 | AT1G31630 | 29.801 | 151 | 104 | 2 | 3 | 152 | 2 | 151 | 5.00e-21 | 87.8 |
| MsG0180004983.01.T01 | AT5G27810 | 37.500 | 96 | 59 | 1 | 29 | 123 | 1 | 96 | 7.51e-19 | 77.4 |
| MsG0180004983.01.T01 | AT5G26580 | 30.128 | 156 | 94 | 3 | 1 | 155 | 1 | 142 | 1.01e-15 | 73.2 |
| MsG0180004983.01.T01 | AT2G40210 | 47.887 | 71 | 37 | 0 | 1 | 71 | 1 | 71 | 3.15e-14 | 69.3 |
| MsG0180004983.01.T01 | AT2G22630 | 42.500 | 80 | 41 | 3 | 1 | 79 | 1 | 76 | 2.19e-11 | 60.1 |
| MsG0180004983.01.T01 | AT2G22630 | 42.500 | 80 | 41 | 3 | 1 | 79 | 1 | 76 | 2.19e-11 | 60.1 |
| MsG0180004983.01.T01 | AT1G47760 | 30.973 | 113 | 67 | 3 | 1 | 110 | 1 | 105 | 3.81e-11 | 58.9 |
Find 32 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACCTCTCAATTATCTTAT+TGG | 0.120149 | 1:-85208997 | None:intergenic |
| ATTATCTTATTGGCTCCTTC+TGG | 0.257805 | 1:-85208987 | None:intergenic |
| TGCCTTCCTTGCTGCGTCAT+CGG | 0.331092 | 1:-85208841 | None:intergenic |
| TGCTGCGTCATCGGGAATAA+AGG | 0.359895 | 1:-85208832 | None:intergenic |
| GCCTTCCTTGCTGCGTCATC+GGG | 0.360745 | 1:-85208840 | None:intergenic |
| TCTGCATGATAAACCTTTCA+TGG | 0.377114 | 1:-85209058 | None:intergenic |
| TTCACCTTTCTTCTCGCCAT+TGG | 0.379933 | 1:-85208804 | None:intergenic |
| GAGAAGATTGATACACTTGA+TGG | 0.449535 | 1:+85209254 | MsG0180004983.01.T01:CDS |
| TTATTGGCTCCTTCTGGATG+TGG | 0.451748 | 1:-85208981 | None:intergenic |
| ACTGAAGAAGTGAAGGATCT+TGG | 0.461931 | 1:+85209197 | MsG0180004983.01.T01:CDS |
| ACCTCTGTCTGAGAATCAAA+TGG | 0.473279 | 1:-85208954 | None:intergenic |
| TCAAAGCAAGGGACAAACTA+AGG | 0.480536 | 1:+85209087 | MsG0180004983.01.T01:CDS |
| AAGCTACAATAAAAGAAAGA+AGG | 0.492405 | 1:+85208863 | MsG0180004983.01.T01:CDS |
| AGCTGGAACATCACAAAGAA+TGG | 0.506592 | 1:-85208913 | None:intergenic |
| GCAACCAATGGCGAGAAGAA+AGG | 0.507401 | 1:+85208800 | None:intergenic |
| ATGAGAATCGTGAGCAAGAG+TGG | 0.520504 | 1:+85209120 | MsG0180004983.01.T01:CDS |
| CTGAAAATTATAGCACAAGC+TGG | 0.522219 | 1:-85208930 | None:intergenic |
| TCCCGATGACGCAGCAAGGA+AGG | 0.534188 | 1:+85208839 | MsG0180004983.01.T01:CDS |
| GCGTGCTGAGAAGAAGTTGA+AGG | 0.552409 | 1:+85209223 | MsG0180004983.01.T01:CDS |
| AAAGAAGGGTCTCATAAAGA+AGG | 0.555375 | 1:+85208878 | MsG0180004983.01.T01:CDS |
| TCCATTTGATTCTCAGACAG+AGG | 0.558728 | 1:+85208953 | MsG0180004983.01.T01:CDS |
| TGCAGACGATTGTCAAAGCA+AGG | 0.562423 | 1:+85209075 | MsG0180004983.01.T01:CDS |
| AGCTACAATAAAAGAAAGAA+GGG | 0.567899 | 1:+85208864 | MsG0180004983.01.T01:CDS |
| CTAGAAATGTGAGCCATGAA+AGG | 0.580946 | 1:+85209045 | MsG0180004983.01.T01:CDS |
| ACTGACTACTGAAGAAGTGA+AGG | 0.595360 | 1:+85209190 | MsG0180004983.01.T01:CDS |
| TGAGAATCGTGAGCAAGAGT+GGG | 0.628956 | 1:+85209121 | MsG0180004983.01.T01:CDS |
| TTATTCCCGATGACGCAGCA+AGG | 0.647670 | 1:+85208835 | MsG0180004983.01.T01:CDS |
| GAGGTGTGGCCACATCCAGA+AGG | 0.649175 | 1:+85208972 | MsG0180004983.01.T01:CDS |
| GAGCCAATAAGATAATTGAG+AGG | 0.656204 | 1:+85208994 | MsG0180004983.01.T01:CDS |
| TTGATTCTCAGACAGAGGTG+TGG | 0.667220 | 1:+85208958 | MsG0180004983.01.T01:CDS |
| GCAGACGATTGTCAAAGCAA+GGG | 0.675137 | 1:+85209076 | MsG0180004983.01.T01:CDS |
| TGCTGAGAAGAAGTTGAAGG+AGG | 0.675328 | 1:+85209226 | MsG0180004983.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACCTCTCAATTATCTTAT+TGG | - | Chr1:85209000-85209019 | None:intergenic | 25.0% |
| ! | AAGCTACAATAAAAGAAAGA+AGG | + | Chr1:85208863-85208882 | MsG0180004983.01.T01:CDS | 25.0% |
| ! | AGCTACAATAAAAGAAAGAA+GGG | + | Chr1:85208864-85208883 | MsG0180004983.01.T01:CDS | 25.0% |
| GAGGTTTAAAAATTTAGCTG+TGG | + | Chr1:85209013-85209032 | MsG0180004983.01.T01:CDS | 30.0% | |
| AAAGAAGGGTCTCATAAAGA+AGG | + | Chr1:85208878-85208897 | MsG0180004983.01.T01:CDS | 35.0% | |
| CTGAAAATTATAGCACAAGC+TGG | - | Chr1:85208933-85208952 | None:intergenic | 35.0% | |
| GAGAAGATTGATACACTTGA+TGG | + | Chr1:85209254-85209273 | MsG0180004983.01.T01:CDS | 35.0% | |
| GAGCCAATAAGATAATTGAG+AGG | + | Chr1:85208994-85209013 | MsG0180004983.01.T01:CDS | 35.0% | |
| TCTGCATGATAAACCTTTCA+TGG | - | Chr1:85209061-85209080 | None:intergenic | 35.0% | |
| !! | ATTATCTTATTGGCTCCTTC+TGG | - | Chr1:85208990-85209009 | None:intergenic | 35.0% |
| ACCTCTGTCTGAGAATCAAA+TGG | - | Chr1:85208957-85208976 | None:intergenic | 40.0% | |
| ACTGAAGAAGTGAAGGATCT+TGG | + | Chr1:85209197-85209216 | MsG0180004983.01.T01:CDS | 40.0% | |
| ACTGACTACTGAAGAAGTGA+AGG | + | Chr1:85209190-85209209 | MsG0180004983.01.T01:CDS | 40.0% | |
| AGCTGGAACATCACAAAGAA+TGG | - | Chr1:85208916-85208935 | None:intergenic | 40.0% | |
| CTAGAAATGTGAGCCATGAA+AGG | + | Chr1:85209045-85209064 | MsG0180004983.01.T01:CDS | 40.0% | |
| TCAAAGCAAGGGACAAACTA+AGG | + | Chr1:85209087-85209106 | MsG0180004983.01.T01:CDS | 40.0% | |
| TCCATTTGATTCTCAGACAG+AGG | + | Chr1:85208953-85208972 | MsG0180004983.01.T01:CDS | 40.0% | |
| !!! | AGTGGGATCTTTTTTTGCTC+AGG | + | Chr1:85209138-85209157 | MsG0180004983.01.T01:CDS | 40.0% |
| ATGAGAATCGTGAGCAAGAG+TGG | + | Chr1:85209120-85209139 | MsG0180004983.01.T01:CDS | 45.0% | |
| GCAGACGATTGTCAAAGCAA+GGG | + | Chr1:85209076-85209095 | MsG0180004983.01.T01:CDS | 45.0% | |
| TGAGAATCGTGAGCAAGAGT+GGG | + | Chr1:85209121-85209140 | MsG0180004983.01.T01:CDS | 45.0% | |
| TGCAGACGATTGTCAAAGCA+AGG | + | Chr1:85209075-85209094 | MsG0180004983.01.T01:CDS | 45.0% | |
| TGCTGAGAAGAAGTTGAAGG+AGG | + | Chr1:85209226-85209245 | MsG0180004983.01.T01:CDS | 45.0% | |
| TTGATTCTCAGACAGAGGTG+TGG | + | Chr1:85208958-85208977 | MsG0180004983.01.T01:CDS | 45.0% | |
| !! | TTATTGGCTCCTTCTGGATG+TGG | - | Chr1:85208984-85209003 | None:intergenic | 45.0% |
| GCGTGCTGAGAAGAAGTTGA+AGG | + | Chr1:85209223-85209242 | MsG0180004983.01.T01:CDS | 50.0% | |
| TGCTGCGTCATCGGGAATAA+AGG | - | Chr1:85208835-85208854 | None:intergenic | 50.0% | |
| TTATTCCCGATGACGCAGCA+AGG | + | Chr1:85208835-85208854 | MsG0180004983.01.T01:CDS | 50.0% | |
| TGCCTTCCTTGCTGCGTCAT+CGG | - | Chr1:85208844-85208863 | None:intergenic | 55.0% | |
| GAGGTGTGGCCACATCCAGA+AGG | + | Chr1:85208972-85208991 | MsG0180004983.01.T01:CDS | 60.0% | |
| GCCTTCCTTGCTGCGTCATC+GGG | - | Chr1:85208843-85208862 | None:intergenic | 60.0% | |
| TCCCGATGACGCAGCAAGGA+AGG | + | Chr1:85208839-85208858 | MsG0180004983.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 85208807 | 85209280 | 85208807 | ID=MsG0180004983.01;Name=MsG0180004983.01 |
| Chr1 | mRNA | 85208807 | 85209280 | 85208807 | ID=MsG0180004983.01.T01;Parent=MsG0180004983.01;Name=MsG0180004983.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|157 |
| Chr1 | exon | 85208807 | 85209280 | 85208807 | ID=MsG0180004983.01.T01:exon:28084;Parent=MsG0180004983.01.T01 |
| Chr1 | CDS | 85208807 | 85209280 | 85208807 | ID=MsG0180004983.01.T01:cds;Parent=MsG0180004983.01.T01 |
| Gene Sequence |
| Protein sequence |
