AlfalfaGEDB | MsG0180005029.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005029.01

KEGG: K11644
GO: GO:0006355
PFAM: PF02671

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005029.01.T01	KEH43393.1	83.784	74	12	0	1	74	1	74	1.28E-34	122

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005029.01.T01	O48686	56.716	67	28	1	8	74	13	78	7.61E-19	82

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005029.01.T01	A0A072VPT4	83.784	74	12	0	1	74	1	74	6.13e-35	122

TFs/TRs:

Gene ID	Type	Classification
MsG0180005029.01.T01	TR	Others

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005029.01.T01	MTR_1g091047	83.784	74	12	0	1	74	1	74	1.55e-38	122
MsG0180005029.01.T01	MTR_8g062700	61.194	67	25	1	8	74	41	106	3.32e-21	85.5
MsG0180005029.01.T01	MTR_8g062700	61.194	67	25	1	8	74	41	106	3.32e-21	85.5
MsG0180005029.01.T01	MTR_5g036670	58.209	67	27	1	8	74	42	107	6.47e-20	82.0
MsG0180005029.01.T01	MTR_1g109550	57.353	68	27	2	5	72	12	77	5.17e-18	72.8
MsG0180005029.01.T01	MTR_3g006150	63.077	65	22	2	9	72	90	153	8.52e-17	70.1
MsG0180005029.01.T01	MTR_6g008970	53.731	67	30	1	8	74	62	127	1.16e-16	72.8
MsG0180005029.01.T01	MTR_3g006065	63.077	65	22	2	9	72	90	153	1.19e-16	70.5
MsG0180005029.01.T01	MTR_1g105960	54.688	64	28	1	11	74	3	65	2.27e-16	67.8
MsG0180005029.01.T01	MTR_1g105955	51.562	64	30	1	11	74	18	80	4.75e-15	65.9
MsG0180005029.01.T01	MTR_1g105955	46.377	69	36	1	4	72	90	157	1.80e-14	64.7
MsG0180005029.01.T01	MTR_6g008995	50.000	66	32	1	9	74	37	101	8.58e-14	64.7
MsG0180005029.01.T01	MTR_1g100260	50.704	71	32	1	5	72	20	90	9.14e-14	61.6
MsG0180005029.01.T01	MTR_4g133470	55.556	63	27	1	10	72	173	234	2.69e-13	62.4
MsG0180005029.01.T01	MTR_4g133300	55.556	63	27	1	10	72	173	234	3.89e-13	62.0
MsG0180005029.01.T01	MTR_1g093310	51.562	64	24	2	8	71	8	64	4.84e-13	58.5
MsG0180005029.01.T01	MTR_1g105010	47.692	65	33	1	8	72	15	78	7.44e-13	58.9
MsG0180005029.01.T01	MTR_1g104940	45.205	73	38	2	3	74	7	78	8.47e-13	58.9
MsG0180005029.01.T01	MTR_7g013410	48.387	62	31	1	13	74	2	62	1.41e-12	58.9
MsG0180005029.01.T01	MTR_3g006010	50.685	73	33	3	1	72	1	71	1.76e-12	60.8
MsG0180005029.01.T01	MTR_8g062350	47.761	67	34	1	8	74	13	78	2.09e-12	59.3
MsG0180005029.01.T01	MTR_1g104960	47.826	69	35	1	6	74	96	163	4.88e-12	58.2
MsG0180005029.01.T01	MTR_1g023820	69.444	36	11	0	39	74	16	51	6.38e-12	56.6
MsG0180005029.01.T01	MTR_3g006125	50.000	74	34	3	1	73	82	153	1.43e-11	57.8
MsG0180005029.01.T01	MTR_5g073430	50.769	65	28	3	13	74	16	79	1.62e-11	55.5
MsG0180005029.01.T01	MTR_3g006185	48.649	74	35	3	1	73	1	72	1.68e-11	56.6
MsG0180005029.01.T01	MTR_3g006185	49.231	65	31	2	9	72	98	161	4.00e-11	55.8
MsG0180005029.01.T01	MTR_1g104980	50.794	63	30	1	12	74	73	134	2.37e-11	56.2
MsG0180005029.01.T01	MTR_3g006105	48.649	74	35	3	1	73	1	72	3.10e-11	56.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005029.01.T01	AT1G24190	56.716	67	28	1	8	74	13	78	5.96e-20	82.0
MsG0180005029.01.T01	AT1G24190	56.716	67	28	1	8	74	13	78	6.83e-20	82.0
MsG0180005029.01.T01	AT1G24190	56.716	67	28	1	8	74	13	78	7.76e-20	82.0
MsG0180005029.01.T01	AT1G59890	59.091	66	26	1	9	74	45	109	8.67e-19	79.0
MsG0180005029.01.T01	AT1G59890	59.091	66	26	1	9	74	45	109	9.46e-19	78.6
MsG0180005029.01.T01	AT1G59890	59.091	66	26	1	9	74	45	109	1.01e-18	78.6
MsG0180005029.01.T01	AT1G59890	59.091	66	26	1	9	74	45	109	1.06e-18	78.6
MsG0180005029.01.T01	AT1G70060	55.224	67	29	1	8	74	13	78	1.40e-18	78.2
MsG0180005029.01.T01	AT1G70060	55.224	67	29	1	8	74	13	78	1.40e-18	78.2
MsG0180005029.01.T01	AT1G10450	59.091	66	26	1	9	74	33	97	1.62e-18	78.2
MsG0180005029.01.T01	AT1G10450	59.091	66	26	1	9	74	33	97	1.62e-18	78.2
MsG0180005029.01.T01	AT1G10450	59.091	66	26	1	9	74	84	148	1.89e-18	77.8
MsG0180005029.01.T01	AT5G15020	53.731	67	30	1	8	74	51	116	2.96e-17	74.3
MsG0180005029.01.T01	AT5G15020	53.731	67	30	1	8	74	51	116	3.08e-17	74.3
MsG0180005029.01.T01	AT3G01320	52.239	67	31	1	8	74	56	121	8.08e-17	73.2
MsG0180005029.01.T01	AT3G01320	52.239	67	31	1	8	74	56	121	8.16e-17	73.2
MsG0180005029.01.T01	AT5G15025	50.746	67	32	1	8	74	46	111	2.12e-14	63.2
MsG0180005029.01.T01	AT1G70030	50.000	66	32	1	9	74	22	86	2.32e-14	63.2
MsG0180005029.01.T01	AT1G70030	50.000	66	32	1	9	74	22	86	6.54e-14	62.4
MsG0180005029.01.T01	AT1G70030	50.000	66	32	1	9	74	11	75	7.16e-14	62.8
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	308	368	8.30e-14	64.7
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	308	368	8.35e-14	64.7
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	308	368	8.35e-14	64.7
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	308	368	8.35e-14	64.7
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	308	368	8.35e-14	64.7
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	308	368	8.35e-14	64.7
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	241	301	8.35e-14	64.7
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	308	368	8.35e-14	64.7
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	308	368	8.35e-14	64.7
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	308	368	8.35e-14	64.7
MsG0180005029.01.T01	AT4G12020	53.968	63	27	2	9	71	308	368	8.35e-14	64.7
MsG0180005029.01.T01	AT1G27250	44.615	65	35	1	8	72	20	83	1.03e-12	58.9
MsG0180005029.01.T01	AT1G27220	41.538	65	37	1	8	72	13	76	6.16e-12	58.2
MsG0180005029.01.T01	AT1G24210	43.077	65	36	1	8	72	13	76	7.52e-12	57.4
MsG0180005029.01.T01	AT1G24200	44.776	67	36	1	8	74	13	78	8.30e-12	57.8

Find 17 sgRNAs with CRISPR-Local

Find 14 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGACATAAAGATTTAATTT+TGG	0.171702	1:+85872872	MsG0180005029.01.T01:CDS
CTCCAGCCCTCAAATATTTC+AGG	0.192555	1:-85872800	None:intergenic
AGAGTGAAGAGACTGTTTAA+AGG	0.353217	1:+85872852	MsG0180005029.01.T01:CDS
CTAGTTTATCATCGCCGATT+TGG	0.368172	1:-85872710	None:intergenic
TAAAACTCCTGAAATATTTG+AGG	0.412179	1:+85872793	MsG0180005029.01.T01:CDS
AAAACTCCTGAAATATTTGA+GGG	0.421671	1:+85872794	MsG0180005029.01.T01:CDS
TTCAACACCTTCCTTCCAAC+TGG	0.425335	1:+85872897	MsG0180005029.01.T01:CDS
CAAATCGGCGATGATAAACT+AGG	0.426757	1:+85872711	MsG0180005029.01.T01:CDS
CTCCTGAAATATTTGAGGGC+TGG	0.470760	1:+85872798	MsG0180005029.01.T01:CDS
AAGATGAAGAACTTCCAAAT+CGG	0.523463	1:+85872696	None:intergenic
AAAAGTAGTAACTAACCAGT+TGG	0.561193	1:-85872912	None:intergenic
TAACTAACCAGTTGGAAGGA+AGG	0.563424	1:-85872904	None:intergenic
CTGAAATATTTGAGGGCTGG+AGG	0.578589	1:+85872801	MsG0180005029.01.T01:CDS
AAATCGGCGATGATAAACTA+GGG	0.599368	1:+85872712	MsG0180005029.01.T01:CDS
GTAGTAACTAACCAGTTGGA+AGG	0.611566	1:-85872908	None:intergenic
TGATAAACTAGGGTTTCTCA+AGG	0.637359	1:+85872722	MsG0180005029.01.T01:CDS
GGAGGAAGAATTGATACAAG+AGG	0.656290	1:+85872819	MsG0180005029.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AGGACATAAAGATTTAATTT+TGG	+	Chr1:85872872-85872891	MsG0180005029.01.T01:CDS	20.0%
!!!	GGACATAAAGATTTAATTTT+GGG	+	Chr1:85872873-85872892	MsG0180005029.01.T01:CDS	20.0%
!	AAAACTCCTGAAATATTTGA+GGG	+	Chr1:85872794-85872813	MsG0180005029.01.T01:CDS	25.0%
!	TAAAACTCCTGAAATATTTG+AGG	+	Chr1:85872793-85872812	MsG0180005029.01.T01:CDS	25.0%
	AAATCGGCGATGATAAACTA+GGG	+	Chr1:85872712-85872731	MsG0180005029.01.T01:CDS	35.0%
	AGAGTGAAGAGACTGTTTAA+AGG	+	Chr1:85872852-85872871	MsG0180005029.01.T01:CDS	35.0%
	TGATAAACTAGGGTTTCTCA+AGG	+	Chr1:85872722-85872741	MsG0180005029.01.T01:CDS	35.0%
	CAAATCGGCGATGATAAACT+AGG	+	Chr1:85872711-85872730	MsG0180005029.01.T01:CDS	40.0%
	GGAGGAAGAATTGATACAAG+AGG	+	Chr1:85872819-85872838	MsG0180005029.01.T01:CDS	40.0%
!	CTAGTTTATCATCGCCGATT+TGG	-	Chr1:85872713-85872732	None:intergenic	40.0%
	CTCCAGCCCTCAAATATTTC+AGG	-	Chr1:85872803-85872822	None:intergenic	45.0%
	CTCCTGAAATATTTGAGGGC+TGG	+	Chr1:85872798-85872817	MsG0180005029.01.T01:CDS	45.0%
	CTGAAATATTTGAGGGCTGG+AGG	+	Chr1:85872801-85872820	MsG0180005029.01.T01:CDS	45.0%
	TTCAACACCTTCCTTCCAAC+TGG	+	Chr1:85872897-85872916	MsG0180005029.01.T01:CDS	45.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	85872699	85872923	85872699	ID=MsG0180005029.01;Name=MsG0180005029.01
Chr1	mRNA	85872699	85872923	85872699	ID=MsG0180005029.01.T01;Parent=MsG0180005029.01;Name=MsG0180005029.01.T01;_AED=0.43;_eAED=0.43;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|74
Chr1	exon	85872699	85872923	85872699	ID=MsG0180005029.01.T01:exon:28347;Parent=MsG0180005029.01.T01
Chr1	CDS	85872699	85872923	85872699	ID=MsG0180005029.01.T01:cds;Parent=MsG0180005029.01.T01

Gene Sequence	>MsG0180005029.01.T01 ATGAAGAACTTCCAAATCGGCGATGATAAACTAGGGTTTCTCAAGGCAGTGAAAGCTGCGTTTGAATATAACAAAATAAAGTATCATCAATTTTTAAAACTCCTGAAATATTTGAGGGCTGGAGGAAGAATTGATACAAGAGGTGTCATGACTAGAGTGAAGAGACTGTTTAAAGGACATAAAGATTTAATTTTGGGATTCAACACCTTCCTTCCAACTGGTTAG
Protein sequence	>MsG0180005029.01.T01 MKNFQIGDDKLGFLKAVKAAFEYNKIKYHQFLKLLKYLRAGGRIDTRGVMTRVKRLFKGHKDLILGFNTFLPTG*