AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005167.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005167.01.T01 MTR_1g093670 67.761 335 51 8 4 297 17 335 3.82e-147 417
MsG0180005167.01.T01 MTR_1g093680 51.566 415 80 5 4 297 52 466 2.44e-132 385
MsG0180005167.01.T01 MTR_5g053430 49.867 377 103 7 4 296 52 426 1.54e-108 323
MsG0180005167.01.T01 MTR_5g053430 49.344 381 102 8 4 296 52 429 1.00e-107 320
MsG0180005167.01.T01 MTR_2g014680 60.000 165 65 1 3 166 46 210 5.73e-63 201
MsG0180005167.01.T01 MTR_7g033320 54.444 180 80 1 4 181 49 228 1.25e-61 204
MsG0180005167.01.T01 MTR_8g069160 57.764 161 67 1 3 162 61 221 5.07e-61 195
MsG0180005167.01.T01 MTR_4g088245 56.970 165 70 1 3 166 61 225 2.17e-60 195
MsG0180005167.01.T01 MTR_8g102240 56.977 172 66 3 3 166 40 211 3.91e-60 199
MsG0180005167.01.T01 MTR_8g102240 56.977 172 66 3 3 166 40 211 4.00e-60 199
MsG0180005167.01.T01 MTR_4g078875 53.448 174 76 2 3 174 41 211 3.50e-59 191
MsG0180005167.01.T01 MTR_2g090735 58.108 148 61 1 3 149 42 189 7.98e-54 177
MsG0180005167.01.T01 MTR_6g084430 33.129 163 80 9 9 163 4 145 7.15e-14 71.2
MsG0180005167.01.T01 MTR_2g080010 36.129 155 72 11 3 150 9 143 7.14e-13 68.6
MsG0180005167.01.T01 MTR_8g059170 34.752 141 76 6 10 149 14 139 2.54e-11 63.9
MsG0180005167.01.T01 MTR_6g012670 31.206 141 81 4 10 149 20 145 6.12e-11 62.8
MsG0180005167.01.T01 MTR_3g088110 35.417 144 72 8 10 150 7 132 8.01e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005167.01.T01 AT1G25580 68.944 161 45 1 6 166 54 209 2.12e-78 246
MsG0180005167.01.T01 AT4G29230 57.310 171 64 2 5 166 43 213 6.77e-62 202
MsG0180005167.01.T01 AT4G29230 56.571 175 67 2 1 166 38 212 1.99e-61 203
MsG0180005167.01.T01 AT4G29230 57.310 171 64 2 5 166 43 213 1.99e-61 203
MsG0180005167.01.T01 AT5G14490 60.265 151 58 1 7 155 52 202 3.30e-58 188
MsG0180005167.01.T01 AT5G56620 54.070 172 67 2 7 166 40 211 4.61e-58 192
MsG0180005167.01.T01 AT1G28470 55.828 163 71 1 5 166 72 234 5.01e-58 189
MsG0180005167.01.T01 AT5G56620 54.070 172 67 2 7 166 40 211 5.58e-58 191
MsG0180005167.01.T01 AT5G14490 60.265 151 58 1 7 155 52 202 5.96e-58 190
MsG0180005167.01.T01 AT5G14490 60.265 151 58 1 7 155 55 205 6.46e-58 190
MsG0180005167.01.T01 AT5G56620 54.070 172 67 2 7 166 40 211 9.62e-58 191
MsG0180005167.01.T01 AT4G28500 54.438 169 69 2 3 166 52 217 1.54e-57 187
MsG0180005167.01.T01 AT5G14490 60.265 151 58 1 7 155 55 205 1.58e-57 189
MsG0180005167.01.T01 AT3G01600 58.940 151 60 1 7 155 52 202 3.96e-56 186
MsG0180005167.01.T01 AT3G18400 34.014 147 79 6 9 153 4 134 7.04e-13 68.6
MsG0180005167.01.T01 AT5G39610 34.270 178 81 8 10 184 20 164 1.41e-12 67.4
MsG0180005167.01.T01 AT1G01720 36.111 144 71 8 10 150 7 132 5.46e-12 65.5
MsG0180005167.01.T01 AT3G04420 32.624 141 79 6 11 148 4 131 4.08e-11 63.2
MsG0180005167.01.T01 AT1G61110 31.868 182 95 8 8 184 14 171 6.30e-11 62.8
MsG0180005167.01.T01 AT5G18270 35.099 151 77 8 3 149 14 147 6.69e-11 62.8
MsG0180005167.01.T01 AT3G12910 31.293 147 81 7 13 154 24 155 8.70e-11 62.0
MsG0180005167.01.T01 AT1G02230 34.483 145 71 8 11 148 4 131 1.00e-10 62.4

Find 67 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCCAGTGTGGTCAAGATTT+TGG 0.220058 1:-87891227 None:intergenic
ATTTATCACTACTCTTAAAA+TGG 0.271764 1:+87889616 MsG0180005167.01.T01:CDS
TCGAACCAAGAAAATGTTTC+CGG 0.290852 1:-87891081 None:intergenic
TGACATGCCACTGAATCTAT+TGG 0.299354 1:+87891050 MsG0180005167.01.T01:CDS
CATCTGATCAAGAGATAATA+TGG 0.323541 1:+87889537 MsG0180005167.01.T01:CDS
TCTTCTAACTGAGGAAGATT+TGG 0.326168 1:-87890934 None:intergenic
GGATCAAATTTAACACCTTT+AGG 0.328542 1:-87889515 None:intergenic
TTTGAATAATTTCTGTTGTT+AGG 0.334781 1:+87889470 None:intergenic
ATGTCTTTCTTTAACTGCTC+TGG 0.340220 1:-87891195 None:intergenic
GAATCTATTGGATTCACTTC+CGG 0.358496 1:+87891062 MsG0180005167.01.T01:CDS
ATAAAATGAATTTAACATAA+TGG 0.373804 1:-87891324 None:intergenic
TATAAGCTTTCACTGCTCTA+TGG 0.392319 1:-87889798 None:intergenic
CTTTATATGTTTCCAGAGAA+TGG 0.394253 1:+87889949 MsG0180005167.01.T01:CDS
TGAACTCCGTTCAAGAAGAT+AGG 0.395219 1:-87889901 None:intergenic
TATGTCCGCTGGCACAAGAC+TGG 0.431620 1:+87889868 MsG0180005167.01.T01:CDS
GCAGTGAAAGCTTATAGTAC+TGG 0.438200 1:+87889805 MsG0180005167.01.T01:CDS
CGACATTCGATTAAGTCAAC+TGG 0.441693 1:+87891275 MsG0180005167.01.T01:CDS
GAGGAAGATTTGGTAGAATC+TGG 0.444547 1:-87890924 None:intergenic
AGAATGGCTACTTAATAGTC+AGG 0.475425 1:+87890954 MsG0180005167.01.T01:CDS
TTAACACCTTTAGGAAGTCC+AGG 0.482399 1:-87889506 None:intergenic
GCCAGTCTCCGAAAAGGCAC+AGG 0.483606 1:+87891118 MsG0180005167.01.T01:CDS
AATATAGGTGTTTCACAAGA+TGG 0.488029 1:+87889757 MsG0180005167.01.T01:intron
CAGAAGAGGATGAGAGGGAA+GGG 0.488806 1:+87890022 MsG0180005167.01.T01:CDS
GTTATGCATCAGTATCATCT+TGG 0.493181 1:+87889997 MsG0180005167.01.T01:CDS
CTTTCGACATATGATCATCT+AGG 0.495768 1:+87891171 MsG0180005167.01.T01:CDS
TAGAAACTCAATTTCATGTA+GGG 0.504338 1:+87891016 MsG0180005167.01.T01:CDS
ACTCATCCTCAGCATTTGCC+AGG 0.505505 1:+87889656 MsG0180005167.01.T01:CDS
TTCAAAGCCAGTCTCCGAAA+AGG 0.507580 1:+87891112 MsG0180005167.01.T01:CDS
TTTCCAGAGAATGGAGGAAA+AGG 0.510158 1:+87889958 MsG0180005167.01.T01:CDS
ACTACTCTTAAAATGGATGA+TGG 0.510952 1:+87889623 MsG0180005167.01.T01:CDS
GTGATAAATGCATCGATGAA+AGG 0.516322 1:-87889602 None:intergenic
AAGAGTTTGCTTATGTCCGC+TGG 0.517104 1:+87889857 MsG0180005167.01.T01:CDS
TGATAAATGCATCGATGAAA+GGG 0.518562 1:-87889601 None:intergenic
ACAGAAGAGGATGAGAGGGA+AGG 0.522455 1:+87890021 MsG0180005167.01.T01:CDS
CGAAAGCGGCGAAGAATATG+TGG 0.522848 1:+87889832 MsG0180005167.01.T01:CDS
TTCTTGAACGGAGTTCAGAA+AGG 0.529603 1:+87889907 MsG0180005167.01.T01:CDS
ATCTTCCTCAGTTAGAAGAA+TGG 0.529692 1:+87890938 MsG0180005167.01.T01:CDS
CATATTATCTCTTGATCAGA+TGG 0.535616 1:-87889536 None:intergenic
TGGCACTTGCTTGCAAAAGT+TGG 0.539348 1:+87889557 MsG0180005167.01.T01:CDS
TCTTTCCAGTCTTGTGCCAG+CGG 0.546974 1:-87889873 None:intergenic
GAGTGGCCTGGACTTCCTAA+AGG 0.554416 1:+87889500 MsG0180005167.01.T01:CDS
TGGAAGATCATTGAGAGCAT+AGG 0.556951 1:-87891304 None:intergenic
GTAGAAACTCAATTTCATGT+AGG 0.560253 1:+87891015 MsG0180005167.01.T01:CDS
TGGAACAGAAGAGGATGAGA+GGG 0.560783 1:+87890017 MsG0180005167.01.T01:CDS
TTAGGTACACAAGAGTGGCC+TGG 0.561833 1:+87889488 MsG0180005167.01.T01:CDS
TTGCTTGCAAAAGTTGGTGA+AGG 0.575602 1:+87889563 MsG0180005167.01.T01:CDS
TGTTGTTAGGTACACAAGAG+TGG 0.579364 1:+87889483 None:intergenic
ATAGTCAGGATGACTGTGAA+GGG 0.580319 1:+87890968 MsG0180005167.01.T01:CDS
ACTAAACCTATCTTCTTGAA+CGG 0.586510 1:+87889895 MsG0180005167.01.T01:CDS
TGATCATATGTCGAAAGACT+AGG 0.588679 1:-87891165 None:intergenic
TTGGAACAGAAGAGGATGAG+AGG 0.602637 1:+87890016 MsG0180005167.01.T01:CDS
AATAGTCAGGATGACTGTGA+AGG 0.603938 1:+87890967 MsG0180005167.01.T01:CDS
TGAAATTGAGTTTCTACCGT+CGG 0.610371 1:-87891009 None:intergenic
CAGTTGACTTAATCGAATGT+CGG 0.615405 1:-87891274 None:intergenic
AATGCATCGATGAAAGGGTG+AGG 0.617026 1:-87889596 None:intergenic
GTATCATCTTGGAACAGAAG+AGG 0.624650 1:+87890008 MsG0180005167.01.T01:CDS
AGTAGCCATTCTTCTAACTG+AGG 0.625387 1:-87890943 None:intergenic
CCAGTCTCCGAAAAGGCACA+GGG 0.626166 1:+87891119 MsG0180005167.01.T01:CDS
CAAGCTCTACATTGCCAGTG+TGG 0.632863 1:-87891239 None:intergenic
AGTGAATCCAATAGATTCAG+TGG 0.638302 1:-87891057 None:intergenic
AGAGCAGTTAAAGAAAGACA+TGG 0.639913 1:+87891197 MsG0180005167.01.T01:CDS
CATATGTCGAAAGACTAGGC+TGG 0.640562 1:-87891161 None:intergenic
TATCCACCTGGCAAATGCTG+AGG 0.644206 1:-87889662 None:intergenic
ATAGTACTGGAAGTCGAAAG+CGG 0.647551 1:+87889818 MsG0180005167.01.T01:CDS
TCCGAAAATTGATCTACCGA+CGG 0.660122 1:+87890993 MsG0180005167.01.T01:CDS
TATATGTTTCCAGAGAATGG+AGG 0.668040 1:+87889952 MsG0180005167.01.T01:CDS
TGCCAAAATCTTGACCACAC+TGG 0.683464 1:+87891225 MsG0180005167.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAGAATGTCTAAAAATAA+TGG - Chr1:87890392-87890411 None:intergenic 15.0%
!! ATACAATAAGAAAATATTAG+TGG - Chr1:87890304-87890323 None:intergenic 15.0%
!!! ATGAGATTAATTTTTAGTTT+TGG + Chr1:87890846-87890865 MsG0180005167.01.T01:intron 15.0%
!! AAGAGTAGTAAACTTAAATT+AGG - Chr1:87890635-87890654 None:intergenic 20.0%
!! ATTTATCACTACTCTTAAAA+TGG + Chr1:87889616-87889635 MsG0180005167.01.T01:CDS 20.0%
!! GAAATATAAAATAACCTAAG+TGG + Chr1:87890875-87890894 MsG0180005167.01.T01:intron 20.0%
!! GCTATATGATTAAACAATAT+AGG + Chr1:87889742-87889761 MsG0180005167.01.T01:intron 20.0%
!!! TAATTTTTAGTTTTGGTTTG+TGG + Chr1:87890853-87890872 MsG0180005167.01.T01:intron 20.0%
!!! TGACATGAAATATTAATTTG+TGG + Chr1:87890436-87890455 MsG0180005167.01.T01:intron 20.0%
! AAGAACAAAATTATCCACTT+AGG - Chr1:87890892-87890911 None:intergenic 25.0%
! AGTTTCTTGTGTAATGAATT+AGG + Chr1:87890483-87890502 MsG0180005167.01.T01:intron 25.0%
! ATTACAAGAATTTGGACTAT+GGG + Chr1:87890807-87890826 MsG0180005167.01.T01:intron 25.0%
! TAGAAACTCAATTTCATGTA+GGG + Chr1:87891016-87891035 MsG0180005167.01.T01:CDS 25.0%
! TTCTAACACCAATTCATATA+GGG + Chr1:87890551-87890570 MsG0180005167.01.T01:intron 25.0%
! TTTCTAACACCAATTCATAT+AGG + Chr1:87890550-87890569 MsG0180005167.01.T01:intron 25.0%
!! ACATTCTTTTGCTTTCTTAT+TGG + Chr1:87890402-87890421 MsG0180005167.01.T01:intron 25.0%
!!! TGTATCTTTTGCTTTGTTTT+TGG + Chr1:87890319-87890338 MsG0180005167.01.T01:intron 25.0%
AATATAGGTGTTTCACAAGA+TGG + Chr1:87889757-87889776 MsG0180005167.01.T01:intron 30.0%
ACTAAACCTATCTTCTTGAA+CGG + Chr1:87889895-87889914 MsG0180005167.01.T01:CDS 30.0%
ACTACTCTTAAAATGGATGA+TGG + Chr1:87889623-87889642 MsG0180005167.01.T01:CDS 30.0%
AGTCCAAATTCTTGTAATCA+AGG - Chr1:87890805-87890824 None:intergenic 30.0%
ATGTATACCCCTATATGAAT+TGG - Chr1:87890562-87890581 None:intergenic 30.0%
CATATTATCTCTTGATCAGA+TGG - Chr1:87889539-87889558 None:intergenic 30.0%
CATCTGATCAAGAGATAATA+TGG + Chr1:87889537-87889556 MsG0180005167.01.T01:CDS 30.0%
CTTTATATGTTTCCAGAGAA+TGG + Chr1:87889949-87889968 MsG0180005167.01.T01:CDS 30.0%
GATTACAAGAATTTGGACTA+TGG + Chr1:87890806-87890825 MsG0180005167.01.T01:intron 30.0%
GGATCAAATTTAACACCTTT+AGG - Chr1:87889518-87889537 None:intergenic 30.0%
GTAGAAACTCAATTTCATGT+AGG + Chr1:87891015-87891034 MsG0180005167.01.T01:CDS 30.0%
GTCCAAATTCTTGTAATCAA+GGG - Chr1:87890804-87890823 None:intergenic 30.0%
TCTAACACCAATTCATATAG+GGG + Chr1:87890552-87890571 MsG0180005167.01.T01:intron 30.0%
TGTTCTTGGTCTACAAAATT+GGG - Chr1:87890200-87890219 None:intergenic 30.0%
TTCTTGTGTAATGAATTAGG+AGG + Chr1:87890486-87890505 MsG0180005167.01.T01:intron 30.0%
TTGTTCTTGGTCTACAAAAT+TGG - Chr1:87890201-87890220 None:intergenic 30.0%
! AAAAGGTGCAAAAACTAACT+GGG + Chr1:87889975-87889994 MsG0180005167.01.T01:CDS 30.0%
! AGGATAGTTTAGCTTAAGAT+AGG + Chr1:87890506-87890525 MsG0180005167.01.T01:intron 30.0%
! ATCATCTTTTCTTCCCAATA+TGG - Chr1:87890087-87890106 None:intergenic 30.0%
! GGATAGTTTAGCTTAAGATA+GGG + Chr1:87890507-87890526 MsG0180005167.01.T01:intron 30.0%
! TGATAAATGCATCGATGAAA+GGG - Chr1:87889604-87889623 None:intergenic 30.0%
! TTCATATAGGGGTATACATT+CGG + Chr1:87890563-87890582 MsG0180005167.01.T01:intron 30.0%
! TTCTTTTGCTTTCTTATTGG+TGG + Chr1:87890405-87890424 MsG0180005167.01.T01:intron 30.0%
!! GTCTAATTTTAACCAATCAG+CGG + Chr1:87890704-87890723 MsG0180005167.01.T01:intron 30.0%
!!! TTTGCTTTGTTTTTGGAAGA+GGG + Chr1:87890326-87890345 MsG0180005167.01.T01:intron 30.0%
!!! TTTTGCTTTGTTTTTGGAAG+AGG + Chr1:87890325-87890344 MsG0180005167.01.T01:intron 30.0%
AAACCACGAAAACCAAAACA+TGG - Chr1:87890733-87890752 None:intergenic 35.0%
AAAGTTCTACCTGAGGAATA+TGG - Chr1:87890231-87890250 None:intergenic 35.0%
ACAAAATTGGGACTGATAAG+AGG - Chr1:87890188-87890207 None:intergenic 35.0%
AGAATGGCTACTTAATAGTC+AGG + Chr1:87890954-87890973 MsG0180005167.01.T01:CDS 35.0%
AGAGCAGTTAAAGAAAGACA+TGG + Chr1:87891197-87891216 MsG0180005167.01.T01:CDS 35.0%
AGTGAATCCAATAGATTCAG+TGG - Chr1:87891060-87891079 None:intergenic 35.0%
ATATGGTGAGTTCTTGTTCT+TGG - Chr1:87890214-87890233 None:intergenic 35.0%
ATCTTCCTCAGTTAGAAGAA+TGG + Chr1:87890938-87890957 MsG0180005167.01.T01:CDS 35.0%
ATGTCTTTCTTTAACTGCTC+TGG - Chr1:87891198-87891217 None:intergenic 35.0%
CTCCCTTGATTACAAGAATT+TGG + Chr1:87890799-87890818 MsG0180005167.01.T01:intron 35.0%
CTTTCGACATATGATCATCT+AGG + Chr1:87891171-87891190 MsG0180005167.01.T01:CDS 35.0%
GAAAGGCTGCAAAAAGATTA+TGG + Chr1:87889924-87889943 MsG0180005167.01.T01:CDS 35.0%
GAAGTTAAAAGTTCTACCTG+AGG - Chr1:87890238-87890257 None:intergenic 35.0%
GAATCTATTGGATTCACTTC+CGG + Chr1:87891062-87891081 MsG0180005167.01.T01:CDS 35.0%
GTTATGCATCAGTATCATCT+TGG + Chr1:87889997-87890016 MsG0180005167.01.T01:CDS 35.0%
TAAAGCTTAGATTATCCACC+TGG - Chr1:87889677-87889696 None:intergenic 35.0%
TATAAGCTTTCACTGCTCTA+TGG - Chr1:87889801-87889820 None:intergenic 35.0%
TATATGTTTCCAGAGAATGG+AGG + Chr1:87889952-87889971 MsG0180005167.01.T01:CDS 35.0%
TATTAGTGGCAGATGATAAC+TGG - Chr1:87890290-87890309 None:intergenic 35.0%
TCGAACCAAGAAAATGTTTC+CGG - Chr1:87891084-87891103 None:intergenic 35.0%
TCTTCTAACTGAGGAAGATT+TGG - Chr1:87890937-87890956 None:intergenic 35.0%
TGAAATTGAGTTTCTACCGT+CGG - Chr1:87891012-87891031 None:intergenic 35.0%
TGATCATATGTCGAAAGACT+AGG - Chr1:87891168-87891187 None:intergenic 35.0%
TGCGAATTAGGTGAGTAATT+CGG - Chr1:87890776-87890795 None:intergenic 35.0%
! CAGTTGACTTAATCGAATGT+CGG - Chr1:87891277-87891296 None:intergenic 35.0%
! GAAAAGGTGCAAAAACTAAC+TGG + Chr1:87889974-87889993 MsG0180005167.01.T01:CDS 35.0%
! GTGATAAATGCATCGATGAA+AGG - Chr1:87889605-87889624 None:intergenic 35.0%
!!! TAACCATGTTTTGGTTTTCG+TGG + Chr1:87890727-87890746 MsG0180005167.01.T01:intron 35.0%
AAAACATGGTTACCGCTGAT+TGG - Chr1:87890719-87890738 None:intergenic 40.0%
AAATTGGGACTGATAAGAGG+AGG - Chr1:87890185-87890204 None:intergenic 40.0%
AAGAACTCACCATATTCCTC+AGG + Chr1:87890219-87890238 MsG0180005167.01.T01:intron 40.0%
AATAGTCAGGATGACTGTGA+AGG + Chr1:87890967-87890986 MsG0180005167.01.T01:CDS 40.0%
AGTAGCCATTCTTCTAACTG+AGG - Chr1:87890946-87890965 None:intergenic 40.0%
ATAGTCAGGATGACTGTGAA+GGG + Chr1:87890968-87890987 MsG0180005167.01.T01:CDS 40.0%
CGACATTCGATTAAGTCAAC+TGG + Chr1:87891275-87891294 MsG0180005167.01.T01:CDS 40.0%
GCAGTGAAAGCTTATAGTAC+TGG + Chr1:87889805-87889824 MsG0180005167.01.T01:CDS 40.0%
GCGAATTAGGTGAGTAATTC+GGG - Chr1:87890775-87890794 None:intergenic 40.0%
GTATCATCTTGGAACAGAAG+AGG + Chr1:87890008-87890027 MsG0180005167.01.T01:CDS 40.0%
TCAAGGGAGAATTGCGAATT+AGG - Chr1:87890788-87890807 None:intergenic 40.0%
TCCGAAAATTGATCTACCGA+CGG + Chr1:87890993-87891012 MsG0180005167.01.T01:CDS 40.0%
TGAACTCCGTTCAAGAAGAT+AGG - Chr1:87889904-87889923 None:intergenic 40.0%
TGACATGCCACTGAATCTAT+TGG + Chr1:87891050-87891069 MsG0180005167.01.T01:CDS 40.0%
TTCTTGAACGGAGTTCAGAA+AGG + Chr1:87889907-87889926 MsG0180005167.01.T01:CDS 40.0%
TTTCCAGAGAATGGAGGAAA+AGG + Chr1:87889958-87889977 MsG0180005167.01.T01:CDS 40.0%
! AATCAGCGGTAACCATGTTT+TGG + Chr1:87890718-87890737 MsG0180005167.01.T01:intron 40.0%
! ACCGTCGGTAGATCAATTTT+CGG - Chr1:87890997-87891016 None:intergenic 40.0%
! ATAGTACTGGAAGTCGAAAG+CGG + Chr1:87889818-87889837 MsG0180005167.01.T01:CDS 40.0%
! CACTTCCGGAAACATTTTCT+TGG + Chr1:87891076-87891095 MsG0180005167.01.T01:CDS 40.0%
! GAGGAAGATTTGGTAGAATC+TGG - Chr1:87890927-87890946 None:intergenic 40.0%
! TCATTTTCCCTGTGCCTTTT+CGG - Chr1:87891129-87891148 None:intergenic 40.0%
! TGGAAGATCATTGAGAGCAT+AGG - Chr1:87891307-87891326 None:intergenic 40.0%
! TTAACACCTTTAGGAAGTCC+AGG - Chr1:87889509-87889528 None:intergenic 40.0%
!! TTGCTTGCAAAAGTTGGTGA+AGG + Chr1:87889563-87889582 MsG0180005167.01.T01:CDS 40.0%
ACTGATAAGAGGAGGTTCAG+TGG - Chr1:87890177-87890196 None:intergenic 45.0%
AGGTTCAGTGGTCGCAAAAT+TGG - Chr1:87890165-87890184 None:intergenic 45.0%
ATTCGCAATAGTTGCTTCCG+CGG - Chr1:87890126-87890145 None:intergenic 45.0%
CATATGTCGAAAGACTAGGC+TGG - Chr1:87891164-87891183 None:intergenic 45.0%
GGTGAGTAATTCGGGTACTT+CGG - Chr1:87890767-87890786 None:intergenic 45.0%
GGTTCAGTGGTCGCAAAATT+GGG - Chr1:87890164-87890183 None:intergenic 45.0%
TCAAGATGTTCCTGAAACCG+CGG + Chr1:87890106-87890125 MsG0180005167.01.T01:intron 45.0%
TGCCAAAATCTTGACCACAC+TGG + Chr1:87891225-87891244 MsG0180005167.01.T01:CDS 45.0%
TGGAACAGAAGAGGATGAGA+GGG + Chr1:87890017-87890036 MsG0180005167.01.T01:CDS 45.0%
TGGCACTTGCTTGCAAAAGT+TGG + Chr1:87889557-87889576 MsG0180005167.01.T01:CDS 45.0%
TTCAAAGCCAGTCTCCGAAA+AGG + Chr1:87891112-87891131 MsG0180005167.01.T01:CDS 45.0%
TTGGAACAGAAGAGGATGAG+AGG + Chr1:87890016-87890035 MsG0180005167.01.T01:CDS 45.0%
! AAGAGTTTGCTTATGTCCGC+TGG + Chr1:87889857-87889876 MsG0180005167.01.T01:CDS 45.0%
! AATGCATCGATGAAAGGGTG+AGG - Chr1:87889599-87889618 None:intergenic 45.0%
! TGCCAGTGTGGTCAAGATTT+TGG - Chr1:87891230-87891249 None:intergenic 45.0%
ACAGAAGAGGATGAGAGGGA+AGG + Chr1:87890021-87890040 MsG0180005167.01.T01:CDS 50.0%
ACTCATCCTCAGCATTTGCC+AGG + Chr1:87889656-87889675 MsG0180005167.01.T01:CDS 50.0%
ATAGTTGCTTCCGCGGTTTC+AGG - Chr1:87890119-87890138 None:intergenic 50.0%
CAAGCTCTACATTGCCAGTG+TGG - Chr1:87891242-87891261 None:intergenic 50.0%
CAGAAGAGGATGAGAGGGAA+GGG + Chr1:87890022-87890041 MsG0180005167.01.T01:CDS 50.0%
CAGCAGCAGCAAGCCATATT+GGG + Chr1:87890071-87890090 MsG0180005167.01.T01:intron 50.0%
CGAAAGCGGCGAAGAATATG+TGG + Chr1:87889832-87889851 MsG0180005167.01.T01:CDS 50.0%
TATCCACCTGGCAAATGCTG+AGG - Chr1:87889665-87889684 None:intergenic 50.0%
TTAGGTACACAAGAGTGGCC+TGG + Chr1:87889488-87889507 MsG0180005167.01.T01:CDS 50.0%
! GCACCTTTTCCTCCATTCTC+TGG - Chr1:87889964-87889983 None:intergenic 50.0%
! TCTTTCCAGTCTTGTGCCAG+CGG - Chr1:87889876-87889895 None:intergenic 50.0%
CATCCTCAGCATTTGCCAGG+TGG + Chr1:87889659-87889678 MsG0180005167.01.T01:intron 55.0%
CCAGCAGCAGCAAGCCATAT+TGG + Chr1:87890070-87890089 MsG0180005167.01.T01:intron 55.0%
GAGTGGCCTGGACTTCCTAA+AGG + Chr1:87889500-87889519 MsG0180005167.01.T01:CDS 55.0%
TATGTCCGCTGGCACAAGAC+TGG + Chr1:87889868-87889887 MsG0180005167.01.T01:CDS 55.0%
! CCAGTCTCCGAAAAGGCACA+GGG + Chr1:87891119-87891138 MsG0180005167.01.T01:CDS 55.0%
!! CCAATATGGCTTGCTGCTGC+TGG - Chr1:87890073-87890092 None:intergenic 55.0%
! CCCTGTGCCTTTTCGGAGAC+TGG - Chr1:87891122-87891141 None:intergenic 60.0%
! GCCAGTCTCCGAAAAGGCAC+AGG + Chr1:87891118-87891137 MsG0180005167.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 87889485 87891335 87889485 ID=MsG0180005167.01;Name=MsG0180005167.01
Chr1 mRNA 87889485 87891335 87889485 ID=MsG0180005167.01.T01;Parent=MsG0180005167.01;Name=MsG0180005167.01.T01;_AED=0.37;_eAED=0.38;_QI=0|0|0|1|0.5|0.66|3|0|306
Chr1 exon 87889485 87889677 87889485 ID=MsG0180005167.01.T01:exon:14321;Parent=MsG0180005167.01.T01
Chr1 exon 87889764 87890050 87889764 ID=MsG0180005167.01.T01:exon:14322;Parent=MsG0180005167.01.T01
Chr1 exon 87890895 87891335 87890895 ID=MsG0180005167.01.T01:exon:14323;Parent=MsG0180005167.01.T01
Chr1 CDS 87889485 87889677 87889485 ID=MsG0180005167.01.T01:cds;Parent=MsG0180005167.01.T01
Chr1 CDS 87889764 87890050 87889764 ID=MsG0180005167.01.T01:cds;Parent=MsG0180005167.01.T01
Chr1 CDS 87890895 87891335 87890895 ID=MsG0180005167.01.T01:cds;Parent=MsG0180005167.01.T01
Gene Sequence

>MsG0180005167.01.T01

TTGTTAGGTACACAAGAGTGGCCTGGACTTCCTAAAGGTGTTAAATTTGATCCATCTGATCAAGAGATAATATGGCACTTGCTTGCAAAAGTTGGTGAAGGAAGTTCAAAACCTCACCCTTTCATCGATGCATTTATCACTACTCTTAAAATGGATGATGGCATTTGTTATACTCATCCTCAGCATTTGCCAGGTGTTTCACAAGATGGAATTGTTTCACACTTTTTCCATAGAGCAGTGAAAGCTTATAGTACTGGAAGTCGAAAGCGGCGAAGAATATGTGGTCAAGAGTTTGCTTATGTCCGCTGGCACAAGACTGGAAAGACTAAACCTATCTTCTTGAACGGAGTTCAGAAAGGCTGCAAAAAGATTATGGTTCTTTATATGTTTCCAGAGAATGGAGGAAAAGGTGCAAAAACTAACTGGGTTATGCATCAGTATCATCTTGGAACAGAAGAGGATGAGAGGGAAGGGAACTATTGGATAATTTTGTTCTTTGTTTGTCTACACCAGATTCTACCAAATCTTCCTCAGTTAGAAGAATGGCTACTTAATAGTCAGGATGACTGTGAAGGGCTTCCGAAAATTGATCTACCGACGGTAGAAACTCAATTTCATGTAGGGATTAATAACATTGACATGCCACTGAATCTATTGGATTCACTTCCGGAAACATTTTCTTGGTTCGAATATTTATTTCAAAGCCAGTCTCCGAAAAGGCACAGGGAAAATGACGAACAAAAGAACCAGCCTAGTCTTTCGACATATGATCATCTAGGACCAGAGCAGTTAAAGAAAGACATGGAAGAGTGCCAAAATCTTGACCACACTGGCAATGTAGAGCTTGACTCAGAATCTTCCGACATTCGATTAAGTCAACTGGTATTTTCCTATGCTCTCAATGATCTTCCATTATGTTAA
Protein sequence

>MsG0180005167.01.T01

LLGTQEWPGLPKGVKFDPSDQEIIWHLLAKVGEGSSKPHPFIDAFITTLKMDDGICYTHPQHLPGVSQDGIVSHFFHRAVKAYSTGSRKRRRICGQEFAYVRWHKTGKTKPIFLNGVQKGCKKIMVLYMFPENGGKGAKTNWVMHQYHLGTEEDEREGNYWIILFFVCLHQILPNLPQLEEWLLNSQDDCEGLPKIDLPTVETQFHVGINNIDMPLNLLDSLPETFSWFEYLFQSQSPKRHRENDEQKNQPSLSTYDHLGPEQLKKDMEECQNLDHTGNVELDSESSDIRLSQLVFSYALNDLPLC*