AlfalfaGEDB | MsG0180005288.01

M. sativa cultivar ZhongmuNo.1 / MsG0180005288.01

KEGG: -
GO: GO:0003677 GO:0006355
PFAM: PF02365

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005288.01.T01	XP_024641473.1	84.661	339	4	4	1	292	1	338	0	570

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005288.01.T01	Q9C8W9	45.051	293	94	6	15	261	5	276	1.31E-75	237

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005288.01.T01	A0A396JTC2	84.661	339	4	4	1	292	1	338	0.0	570

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TFs/TRs:

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Gene ID	Type	Classification
MsG0180005288.01.T01	TF	NAC

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005288.01.T01	MTR_1g096430	83.792	327	5	4	13	292	1	326	0.0	546
MsG0180005288.01.T01	MTR_8g076110	39.937	318	107	9	15	284	2	283	1.68e-64	205
MsG0180005288.01.T01	MTR_5g021710	44.574	258	78	6	15	224	4	244	3.74e-63	203
MsG0180005288.01.T01	MTR_4g036030	56.410	156	23	1	13	123	1	156	4.75e-54	179
MsG0180005288.01.T01	MTR_4g101680	56.410	156	23	1	13	123	1	156	5.67e-54	180
MsG0180005288.01.T01	MTR_5g012080	52.532	158	28	1	13	123	1	158	5.70e-50	169
MsG0180005288.01.T01	MTR_8g024480	74.510	102	26	0	10	111	6	107	3.98e-49	169
MsG0180005288.01.T01	MTR_2g062730	52.597	154	27	1	16	123	7	160	4.88e-49	166
MsG0180005288.01.T01	MTR_4g035590	52.349	149	27	1	19	123	8	156	2.14e-47	162
MsG0180005288.01.T01	MTR_2g064090	63.441	93	33	1	19	111	6	97	1.69e-37	136

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180005288.01.T01	AT1G71930	45.051	293	94	6	15	261	5	276	1.33e-76	237
MsG0180005288.01.T01	AT1G71930	45.051	293	94	6	15	261	5	276	1.33e-76	237
MsG0180005288.01.T01	AT5G66300	40.397	302	107	9	14	264	7	286	6.15e-61	196
MsG0180005288.01.T01	AT4G36160	39.628	323	101	9	12	261	3	304	6.94e-61	198
MsG0180005288.01.T01	AT4G36160	39.628	323	101	9	12	261	3	304	6.94e-61	198
MsG0180005288.01.T01	AT4G36160	39.628	323	101	9	12	261	7	308	7.14e-61	198
MsG0180005288.01.T01	AT2G18060	44.355	248	77	6	15	215	4	237	1.70e-60	197
MsG0180005288.01.T01	AT2G18060	44.355	248	77	6	15	215	4	237	1.70e-60	197
MsG0180005288.01.T01	AT1G12260	35.362	345	119	9	13	261	1	337	2.88e-53	179
MsG0180005288.01.T01	AT1G12260	48.913	184	31	3	13	139	1	178	3.13e-52	174

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Find 55 sgRNAs with CRISPR-Local

Find 162 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAAGCCAAGTCCAAGTCAT+AGG	0.450680	1:+89711783	MsG0180005288.01.T01:CDS
AAAATATCTGTAATTGATAA+AGG	0.402136	1:-89711887	None:intergenic
AAACCAAAATACTCTTGTTA+TGG	0.379475	1:+89711997	MsG0180005288.01.T01:CDS
AAACTGTTGATCATTGCTAA+AGG	0.486770	1:-89711969	None:intergenic
AAACTTGTACCTTCAGCTGC+AGG	0.517860	1:-89711938	None:intergenic
AAAGAAAATACTCATCACAA+TGG	0.626769	1:+89712079	MsG0180005288.01.T01:CDS
AAAGATAAGAAATATCCTAC+AGG	0.562663	1:+89710980	MsG0180005288.01.T01:CDS
AACTGTTGATCATTGCTAAA+GGG	0.376851	1:-89711968	None:intergenic
AAGAAGGATGGGTTGTGTGC+AGG	0.445621	1:+89711753	MsG0180005288.01.T01:CDS
AAGAATATTGCAGTGAAGAA+AGG	0.454802	1:+89712113	MsG0180005288.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAATTACACTAGCTAGCT+AGG	-	Chr1:89710039-89710058	None:intergenic	30.0%
AAAATTACACTAGCTAGCTA+GGG	-	Chr1:89710038-89710057	None:intergenic	30.0%
AAACTGTTGATCATTGCTAA+AGG	-	Chr1:89711972-89711991	None:intergenic	30.0%
AACTGTTGATCATTGCTAAA+GGG	-	Chr1:89711971-89711990	None:intergenic	30.0%
AAGAATATTGCAGTGAAGAA+AGG	+	Chr1:89712113-89712132	MsG0180005288.01.T01:CDS	30.0%
AATAATAGTTACCCACACTA+AGG	-	Chr1:89709097-89709116	None:intergenic	30.0%
ACAATGGACAAGTAATTGAT+TGG	+	Chr1:89712152-89712171	MsG0180005288.01.T01:CDS	30.0%
ACTGAAATCAGTATTATGGA+TGG	+	Chr1:89709492-89709511	MsG0180005288.01.T01:intron	30.0%
AGTAATTGATTGGAAAAGCT+TGG	+	Chr1:89712162-89712181	MsG0180005288.01.T01:CDS	30.0%
ATCTCTACAAAATTGAACCA+TGG	+	Chr1:89710731-89710750	MsG0180005288.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	89709019	89712315	89709019	ID=MsG0180005288.01;Name=MsG0180005288.01
Chr1	mRNA	89709019	89712315	89709019	ID=MsG0180005288.01.T01;Parent=MsG0180005288.01;Name=MsG0180005288.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|0\|0\|1\|0.66\|0.5\|4\|0\|298
Chr1	exon	89709019	89709039	89709019	ID=MsG0180005288.01.T01:exon:47822;Parent=MsG0180005288.01.T01
Chr1	exon	89710589	89710763	89710589	ID=MsG0180005288.01.T01:exon:47823;Parent=MsG0180005288.01.T01
Chr1	exon	89710927	89711063	89710927	ID=MsG0180005288.01.T01:exon:47824;Parent=MsG0180005288.01.T01
Chr1	exon	89711752	89712315	89711752	ID=MsG0180005288.01.T01:exon:47825;Parent=MsG0180005288.01.T01
Chr1	CDS	89709019	89709039	89709019	ID=MsG0180005288.01.T01:cds;Parent=MsG0180005288.01.T01
Chr1	CDS	89710589	89710763	89710589	ID=MsG0180005288.01.T01:cds;Parent=MsG0180005288.01.T01
Chr1	CDS	89710927	89711063	89710927	ID=MsG0180005288.01.T01:cds;Parent=MsG0180005288.01.T01
Chr1	CDS	89711752	89712315	89711752	ID=MsG0180005288.01.T01:cds;Parent=MsG0180005288.01.T01

Gene Sequence

>MsG0180005288.01.T01

ATGACATCAAATCAAATTACTGAGAGATCATCATCAATGGATATGGAATCATGTGTACCTCCAGGGTTTAGATTTCATCCAACAGAAGAAGAACTTGTAGGGTATTACCTCAACAGGAAGATTAACTCACTCAAAATTGATCTAGATGTTATTGTTGAGGTTGATCTCTACAAAATTGAACCATGGGACATTCAAGATAGATGCAAGCTAGGATATGAGCAACAGAATGAGTGGTACTTTTTCAGCCACAAAGATAAGAAATATCCTACAGGAACAAGAACTAATAGAGCCACTGCTGCTGGATTTTGGAAAGCTACTGGAAGGGATAAAGCTGAAGAAGGATGGGTTGTGTGCAGGGCATTTAAAAAGCCAAGTCCAAGTCATAGGCCAGGTTATGAACCATGGTATAGTAATCAACAACAACCACAGTATTTCAGATCAGATGATCAAAGCTATGCAACTACAAGACCTTTATCAATTACAGATATTTTGCATGAAAATCGTTTACTCCTTCAAAATCCTGCAGCTGAAGGTACAAGTTTTAGTCACCCCTTTAGCAATGATCAACAGTTTCTATCAAACCAAAATACTCTTGTTATGGACAATAAACAACTCATTGAACTACCACAGTTAGATAGTCCAACAACAAGTTTTGCAATCAAAGAAAATACTCATCACAATGGACTCACAATAGAAGAATATTGCAGTGAAGAAAGGAGCATTAATAATAATAACAATGGACAAGTAATTGATTGGAAAAGCTTGGACAATTTGTTCAGTTCAAATCAATTTACAGATACAGCTAATTACTTTTCAAATCAAAATAGCATGCCATTGATGATACCTCATAGTCAACATGAGCTACAATCTCATCAAATCAAGCTAATCATATCTTAG

Protein sequence

>MsG0180005288.01.T01

MTSNQITERSSSMDMESCVPPGFRFHPTEEELVGYYLNRKINSLKIDLDVIVEVDLYKIEPWDIQDRCKLGYEQQNEWYFFSHKDKKYPTGTRTNRATAAGFWKATGRDKAEEGWVVCRAFKKPSPSHRPGYEPWYSNQQQPQYFRSDDQSYATTRPLSITDILHENRLLLQNPAAEGTSFSHPFSNDQQFLSNQNTLVMDNKQLIELPQLDSPTTSFAIKENTHHNGLTIEEYCSEERSINNNNNGQVIDWKSLDNLFSSNQFTDTANYFSNQNSMPLMIPHSQHELQSHQIKLIIS*